Uptake in cells. Medical search. Frequent questions

Although the ability of both vertebrate scavenger endothelial cells (SECs) and {PCs} to sequester colloidal and soluble macromolecules has been demonstrated the molecular mechanism of this function remains to be investigated. (amrita.edu)
Rose, J.M. and Audus, K.L. (1999) AT1 receptors mediate angiotensin II uptake and transport by brain microvessel endothelial cells in primary culture. (ku.edu)
This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. (lu.se)
Injury to endothelial cells by lipid-soluble smoke particles or ultraviolet light, which reduced total cellular protein by 15-25%, enhanced the endothelial cell release of the substances stimulating 125I-low density lipoprotein uptake. (lu.se)
article{e328883f-12c1-4d3a-a8e7-8700ca87f7ac, abstract = {{This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. (lu.se)
Uptake of chemically modified low density lipoproteins in vivo is mediated by specific endothelial cells. (rupress.org)
Because macrophages, Kupffer cells, and to a lesser extent, endothelial cells metabolize these modified lipoproteins in vitro, it was of interest to determine whether endothelial cells or macrophages could be responsible for the in vivo uptake of these lipoproteins. (rupress.org)
Uptake of AcAc LDL by endothelial cells of the liver, spleen, and bone marrow was confirmed by transmission electron microscopy. (rupress.org)
Isolated sinusoidal endothelial cells from the rat liver displayed saturable, high affinity binding of AcAc LDL (Kd = 2.5 X 10(-9) M at 4 degrees C), and were shown to degrade AcAc LDL 10 times more effectively than aortic endothelial cells. (rupress.org)
These data indicate that specific sinusoidal endothelial cells, not the macrophages of the reticuloendothelial system, are primarily responsible for the removal of these modified lipoproteins from the circulation in vivo. (rupress.org)
The uptake of labeled MPs in the presence of protein S and Gas6 in human aortic endothelial cells (HAEC) and human umbilical vein endothelial cells (HUVEC) was monitored by flow cytometry, western blotting and confocal/electron microscopy. (lu.se)

MEVs display increased time- and dose-dependent cellular uptake compared to fEVs. (nih.gov)
We report here the functional characterization of Drosophila larval {PCs} with important insights into their cellular uptake pathways. (amrita.edu)
The capability of cell-penetrating peptides (CPPs) to transport cargos over different cellular membranes both in vitro and in vivo have drawn major attention in the past decade. (avhandlingar.se)
The translocation of CPPs into plant protoplasts might open up a new method for transformation of plant cells.Next, the cell-penetrating ability of the novel peptide YTA2 was characterized and it was established that chemical coupling between YTA2 and the protein cargo is not needed for the transport of the cargo over the cellular membrane in vitro. (avhandlingar.se)
A complete lack of this protein could lead to considerably more intake of fatty acids by fat-storing cells from the cellular environment. (azolifesciences.com)
Cellular uptake studies were performed using different human cell lines such as SH-SY5Y (Epithelial Neuroblastoma), U-87 MG (Epithelial Glioblastoma) and hCMEC/D3 (Brain Endothelial) cell lines to visualise uptake of the different polysaccharide coated UCNPs using confocal microscopy. (edu.au)
Recent experiments have also provided convincing evidence on the importance of elastic deformation in cellular uptake of nanoparticles. (uga.edu)
So far, relatively little attention has been devoted to the effects of elastic properties on cellular uptake of nanoparticle such as vesicles via computational simulation although several theoretical models have been proposed to understand the effect of elastic deformation of particles on cellular uptake from the perspective of adhesive wrapping of an elastic, deformable vesicle by a lipid membrane. (uga.edu)
If cellular ferritin is even further increased through 1 h incubation (24 h prior to H2O2 exposure) of both cell types with hemin, FeSO4, or exogenous spleen apoferritin itself (24 h), marked resistance to H2O2-mediated cytotoxicity is manifest. (mtak.hu)
The incorporation of Cu+ ions in the polymer greatly improves its cellular uptake and endosomal escape. (bvsalud.org)
In summary, our study highlights the impact of thermoresponsivity on the cellular uptake mechanisms which has to be taken into account if the tNGs are used as a drug delivery system. (hereon.de)
Accordingly, understanding the underlying mechanism of cellular uptake is an important step toward understanding the biological fate of nanoparticles, both the favorable and adverse aspects [1] . (materialstoday.com)
In addition to parameters such as size and shape, chemical functionalities on the surface seem to play a critical role in binding to cell membrane and subsequent cellular uptake [2] and [3] . (materialstoday.com)
Consequently, the widespread theory that the preferential cellular uptake of positively charged nanoparticles over neutral or negatively charged nanoparticles is due to favorable electrostatic interactions with cell membrane seems simplistic and reductive. (materialstoday.com)
Resistin suppresses the ability of INSULIN to stimulate cellular GLUCOSE uptake. (bvsalud.org)

Analysis of Fucoidan-UCNP nanoparticle uptake showed increasing UCNP uptake into macrophage cells over 16 hrs. (edu.au)
Amphiphilic nanoparticle uptake was however dramatically lower than that of a model system (Fluospheres) and similar to PEG-coated colloids reported in the literature, which are considered "stealth. (consensus.app)

Although the recognition of CpG DNA by B cells and plasmacytoid dendritic cells is mediated by TLR 9, these cell types differ in their ability to bind and respond to structurally distinct classes of CpG oligonucleotides. (fda.gov)
Milk fat globule - epidermal growth factor 8 (MFG-E8) is a glycoprotein secreted by activated macrophages and immature dendritic cells. (soton.ac.uk)
receptors and conferred enhanced phagocytosis by both macrophages and immature dendritic cells (DN-MFG-E8-Fc) in a dose-dependent manner. (soton.ac.uk)
Cross-presentation of cell-associated antigen by bone marrow-derived dendritic cells (BMDCs) was determined by CD8+ T cell proliferation assays in vitro and in vivo. (soton.ac.uk)
The tyrosine kinase receptors Tyro3, Axl and Mer (TAMs) and their ligands protein S and Gas6 are involved in the uptake of PS-exposing apoptotic cells in macrophages and dendritic cells. (lu.se)
Although a large number of studies has investigated the relation between allergies and infection, little is known about the influence of the exposure to infections on antigen uptake by dendritic cells (DCs). (elsevierpure.com)
As it has been indicated that nanogels possess a high ability to penetrate the stratum corneum, it cannot be excluded that nanogels interact with dermal dendritic cells, especially in diseased skin. (hereon.de)
In this study the potential crosstalk of the thermoresponsive nanogels (tNGs) with the dendritic cells of the skin was investigated with the aim to determine the immunotoxicological properties of the nanogels. (hereon.de)
Interestingly, specific uptake mechanisms of the tNGs by the dendritic cells were depending on the nanogels cloud point temperature (Tcp), which determines the phase transition of the nanoparticle. (hereon.de)

receptors (Fc?Rs) reduced their ability to induce CD8+ T cell proliferation in vivo, suggesting the blockade of a mechanism which is intrinsic for DC maturation or migration. (soton.ac.uk)
The polysaccharide nanoparticles were internalised differently in the cell lines with and without LPS induced inflammation, indicating the possible involvement of specific sugar receptors in the uptake mechanism. (edu.au)
The mechanisms described here refute the general assumption that B-cell presentation of self-antigens requires uptake via specific B-cell receptors, and may be important for maintenance of tolerance as well as for driving T-cell responses in autoimmune diseases. (regionh.dk)
2003), and (c) availability of T lymphocytes way how skin sensitization is dealt with in toxicological with T-cell receptors specific for the hapten-peptide con- risk assessment: while older test systems aimed at pure jugates formed (Budinger et al. (cdc.gov)

Manchester scientists have found that gentle heating of targeted nano-sized drug parcels more effectively in deliver them to tumour cells - resulting in an improvement in survival rates ( Journal of Controlled Release , "Monoclonal antibody-targeted, temperature-sensitive liposomes: In vivo tumor chemotherapeutics in combination with mild hyperthermia" ). (cytofluidix.com)
The effectiveness of these liposomes has been further improved by engineering them to contain molecules (monoclonal antibodies) on their surface that allow them to better target cancer cells in combination to making them temperature-sensitive so that they release their therapeutic drug content upon mild heating. (cytofluidix.com)
The team compared liposomes with and without the ability to actively target cancer cells. (cytofluidix.com)
They found that in combination with mild heating, the actively targeted liposomes showed greater uptake in tumour tissue in mice than those without targeting ability. (cytofluidix.com)

This allows the drug to more effectively localize into cancer tissue and reduces side-effects by limiting drug-infused liposome uptake in healthy cells. (cytofluidix.com)

Uptake is blocked by protonophores such as CCCP (but not arsenate), and evidence for a proton symport mechanism has been presented. (wikipedia.org)
This uptake was inhibited by pre-incubation with the monosaccharide component of fucoidan, indicating a specific fucose receptor mediated mechanism for Fucoidan-UCNP uptake. (edu.au)
Our studies suggest lipid metabolism may be a critical survival mechanism of CLL cells and recent data demonstrates upregulation of key proteins involved in this [Thurgood et al. (edu.au)
2019]. The aim of this work is to determine the preferred nutrient 213 source for CLL, its mechanism of uptake and utilization within the cells. (edu.au)
The uptake and accumulation of pathological α-synuclein aggregates in neuronal cells is thought to be a major underlying mechanism in the pathogenesis of Parkinson's disease. (zenodo.org)
The study points to caveolae-mediated endocytosis as being the major tNGs uptake mechanism at 37 °C, which is above the Tcp of the tNGs. (hereon.de)
Remarkably, an additional uptake mechanism, beside caveolae-mediated endocytosis, was observed at 29 °C, which is the Tcp of the tNGs. (hereon.de)

The uptake of nanoparticles by cells can be viewed as a two-step process: binding to the cell membrane and internalization. (materialstoday.com)

Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. (nature.com)
This protocol describes an assay to quantify the uptake of these aggregates by neuronal cells by monitoring the uptake of fluorescently labelled α-synuclein preformed fibrils (PFFs) in dopaminergic (DA) neural progenitor cells (NPCs) derived from induced pluripotent stem cells (iPSCs). (zenodo.org)
Does Screen Quest™ Colorimetric Glucose Uptake Assay Kit work with X cell type? (aatbio.com)
Our Screen Quest™ Colorimetric Glucose Uptake Assay Kit can be used to measure glucose uptake in cells that have glucose transporters. (aatbio.com)

Gross primary production (GPP)-the uptake of carbon dioxide (CO 2 ) by leaves, and its conversion to sugars by photosynthesis-is the basis for life on land. (nature.com)
Many of the studies found that on the European scale, the forests protected themselves by reducing their transpiration and growth, thus leading to a decreased uptake of carbon dioxide. (sonnenseite.com)

In PND8, ex vivo analysis of hippocampal and frontal cortex slices evaluated cell viability and susceptibility to in vitro glutamate challenge. (iasp-pain.org)
In adult rats, behavioral parameters related to anxiety-like behavior, short-term memory, and locomotor activity (PND60-62) and ex vivo analysis of cell viability, membrane permeability, glutamate uptake, and susceptibility to in vitro glutamate challenge in hippocampal and cortical slices from PND65. (iasp-pain.org)
Ex vivo analysis of brain slices showed isoflurane neonatal exposure selectively decreased cell viability and glutamate uptake in cortical slices, but it did not alter hippocampal slice viability or glutamate uptake (PND65). (iasp-pain.org)
Moreover, the homing capacity of the PEGA-pVEC and CREKA-pVEC was conserved in vivo, where the conjugates mainly accumulated in blood vessels in breast tumor tissue and were subsequently translocated into cells. (avhandlingar.se)
A histological examination of tissue sections, undertaken after the administration of AcAc LDL or Ac LDL (labeled with either 125I or a fluorescent probe) to rats, dogs, or guinea pigs, was used to identify the specific cells binding and internalizing these lipoproteins in vivo. (rupress.org)

Here, two recombinant dominant-negative MFG-E8 proteins were generated: one which is shown to inhibit PS-dependent apoptotic cell uptake by macrophages by over 40% (DN-MFG-E8), and a second which re-directed apoptotic cells through Fc? (soton.ac.uk)
Amphiphilic hydrogel nanoparticles show potential as long-circulating drug carriers, with in vitro uptake by J774A.1 macrophages. (consensus.app)
When introduced to a static cell culture of adhered J774A.1 macrophages, the cells internalized these hydrogel nanoparticles in a dose- and time- dependent manner through clathrin-mediated and other pathways. (consensus.app)

Although MSC-derived EVs (mEVs) are beneficial for acute lung injury and pulmonary fibrosis, mechanisms of mEV uptake by lung fibroblasts and their effects on myofibroblastic differentiation have not been established. (nih.gov)
Impaired phagocytic function of glial cells during later stages of AD likely contributes to worsened disease outcome, but the underlying mechanisms of how this occurs remain unknown. (ucl.ac.uk)
Thus, these biocompatible polysaccharide-coated luminescent UCNPs have the potential to explore intracellular mechanisms via live cell quantitative imaging and targeted delivery. (edu.au)

Published 15 August 2016 In this study, we provide new insight into how cancer cells adapt to stress factors present within the tumor microenvironment through increased uptake of lipoproteins. (lu.se)

These data suggest that the interaction of Thy-1 with beta integrins mediates mEV uptake by lung fibroblasts, which blocks myofibroblastic differentiation, and that mEVs are enriched for miRs that target profibrotic genes up-regulated in IPF fibroblasts. (nih.gov)

Colominic acid coated UCNPs were imaged at single particle resolution in PC12 (pre-neuronal cells) using the super resolution techniques of Super-linear excitation-emission microscopy (SEE) and Stimulated emission depletion (STED). (edu.au)

Apoptotic cells are cleared rapidly by professional phagocytes thus preventing the release of potentially inflammatory or immunogenic material into the surrounding environment. (soton.ac.uk)
It facilitates the uptake of apoptotic cells by acting as a bridging molecule between integrins on the phagocyte and phosphatidylserine (PS) on the apoptotic cell surface. (soton.ac.uk)
Loading BMDCs with apoptotic cells via a PS-independent pathway or through Fc? (soton.ac.uk)
Apoptotic cells treated with DN-MFG-E8-Fc protein resulted in upregulation of costimulatory molecules, CD86 and CD70, when BMDCs were deficient for the inhibitory Fc?R, Fc?RIIb. (soton.ac.uk)

The anionic Cr (VI) diffuses readily through the red blood cell (RBC) membrane and is bound by the haemoglobin probably after its rapid reduction to the cationic trivalent state within the RBC (Gray and Sterling, 1950). (strath.ac.uk)
EHD2 is found inside muscle and fat cells as it is a membrane protein. (azolifesciences.com)
Small flask-shaped membrane structures named caveolae are formed when the cell envelope folds inward. (azolifesciences.com)
These transpositions either tend to stay on the cell membrane surface or pinch off and transport foreign material such as fatty acids into the cell. (azolifesciences.com)
TRK transporters, responsible for the bulk of K+ accumulation in plants, fungi, and bacteria, mediate ion currents driven by the large membrane voltages (-150 to -250 mV) common to non-animal cells. (wikipedia.org)
The increase in Ps of cytoplasm would allow free water to diffuse across the membrane, allowing the cell to absorb water. (sekimo.com)
The results indicated that the decrease in K+ uptake due to Ca2+ starvation was caused by the repression of the cation-stimulated ATPase activity associated with the plasma membrane fraction. (eurekamag.com)
Regarding cell membrane, surface charge and membrane potential are often confused and a sharper distinction should be done between the two as they refer to different concepts. (materialstoday.com)
This is why cell membrane is generally considered as globally negatively charged [5] . (materialstoday.com)
The accepted explanation lies in the fact that electrostatic interactions are favored with cell membrane that is negatively charged. (materialstoday.com)
Amnionless attaches (binds) to cubilin, anchoring cubilin to the cell membrane. (medlineplus.gov)
Published 20 April 2016 Hypoxia modulates global membrane proteome turnover in cancer cells, providing opportunities for tumor specific drug delivery. (lu.se)

As the uptake improves, the cost will come down. (miningweekly.com)

Iron absorption in Arabidopsis root epidermal cells requires the IRT1 transporter that also allows the entry of certain non-iron metals, such as Zn, Mn, and Co. Recent work demonstrated that IRT1 endocytosis and degradation are controlled by IRT1 non-iron metal substrates in a ubiquitin-dependent manner. (nih.gov)
It was demonstrated that endothelial cell-released non-dialysable (molecular weight cut off 12-14000) substances significantly stimulated 125I-low density lipoprotein uptake and degradation by smooth muscle cells. (lu.se)
The increase in 125I-low density lipoprotein uptake and degradation by smooth muscle cells could be dissociated from cell proliferation. (lu.se)
However, in endothelial cell-smooth muscle cell co-culture 125I-low density lipoprotein uptake and degradation by smooth muscle cells were not stimulated. (lu.se)

In contrast, repeated, more chronic, exposure effector cells or chemotherapeutic agents, an effect derived from their increased synthesis and accumulation of the intracellular iron scavenger, ferritin. (mtak.hu)

Upregulation of miR-489-3p and miR-630 inhibits oxaliplatin uptake in renal cell carcinoma by targeting OCT2. (particle-metrix.com)

It was hypothesised that manipulation of this clearance pathway, which is normally employed to prevent autoimmunity, may alleviate immuno-suppression of apoptotic tumour cells and promote the expansion of a tumour antigen-specific T cell response. (soton.ac.uk)
Under serum-free conditions we observed that 3-4% of circulating B cells from healthy donors were capable of binding the MS-associated self-antigen myelin basic protein (MBP) and of presenting the immunodominant peptide MBP85-99, as determined by staining with the mAb MK16 recognising the peptide presented by HLA-DR15-positive cells. (regionh.dk)
In this study, we examined the effect of lipopolysaccharide (LPS) priming in early life on the antigen uptake ability of DCs by using an original mouse model. (elsevierpure.com)
LPS priming in juvenile mice decreased the migration of antigen-capturing CD11c + cells in the lymph nodes, but not in aged mice. (elsevierpure.com)
Besides, the bone marrow-derived DCs (BMDCs) from juvenile LPS-primed mice had the poor antigen uptake ability, and constitutively produced NO through the inducible nitric oxide synthase (iNOS). (elsevierpure.com)
Interestingly, the LPS priming-induced poor antigen uptake of BMDCs was mimicked by the NO donor, and recovered by the iNOS inhibitor. (elsevierpure.com)

A single isoflurane (3%, 2 h) exposure at PND7 did not acutely alter cell viability in cortical and hippocampal slices of infant rats (PND8) per se and did not alter slice susceptibility to in vitro glutamate challenge. (iasp-pain.org)
Isoflurane exposure did not alter in vitro glutamate-induced neurotoxicity to slices, and isoflurane exposure caused no significant long-term damage to cell membranes in hippocampal or cortical slices. (iasp-pain.org)
Both, PEGA-pVEC and CREKA-pVEC were taken up by different breast cancer cells in vitro. (avhandlingar.se)
In vitro uptake of amphiphilic, hydrogel nanoparticles by J774A.1 cells. (consensus.app)
We here report improved synthesis and in vitro interactions of amphiphilic hydrogel nanoparticles with the macrophage cell line J774A.1. (consensus.app)

Tumor cell heme uptake induces ferritin synthesis resulting in altered oxidant sensitivity: possible role in chemotherapy efficacy. (mtak.hu)

With the new understanding, Matthaeus and her team, which includes scientists from the MDC research group headed by Professor Oliver Daumke, predict that EHD2 regulates a metabolic pathway that controls the uptake of fatty acids in fat cells. (azolifesciences.com)
Cadmium uptake increased by 13% in Gram-positive bacteria after addition of nutrients, suggesting some metabolic uptake of cadmium. (njit.edu)

Removal or blocking of Thy-1, or blocking Thy-1-beta integrin interactions, decreased mEV uptake and prevented suppression of myofibroblastic differentiation. (nih.gov)
The student will study the effect of the stiffness of coating ligand on the nanoparticle surface by tuning the types of coating polymers on its penetration efficiency and translocation time into cells via an integrated computational and experimental methodology, therefore providing useful clues to understand the fundamental role of mechanical stiffness in the cell-nanoparticle interactions. (uga.edu)
The investigation of nanoparticle/cell interactions is a crucial issue with regard to two fields: nanomedicine and nanotoxicology. (materialstoday.com)
It is commonly admitted that nanoparticles physico-chemical features are determining factors in nanoparticle/cell interactions and consequently influence cell behavior. (materialstoday.com)

To better understand how metal uptake is regulated, we identified IRT1-interacting proteins in Arabidopsis roots by mass spectrometry and established an interactome of IRT1. (nih.gov)
The delivery of proteins into cells by mere coincubation with CPPs is an improvement, since the chemical coupling between the CPP and the cargo molecule, which adds time-consuming synthesis and purification steps, can be omitted.Finally, by conjugating each of the two breast tumor homing peptides, the cyclic cCPGPEGAGC (PEGA) or the linear CREKA peptide, to the CPP pVEC, two cell-penetrating peptides with homing properties were obtained. (avhandlingar.se)
We speculate that acute exposure of tumors to iron (e.g., derived from heme-proteins in hemorrhagic cancerous lesions) may increase sensitivity of some cancer cells, particularly those relatively low in endogenous ferritin, to oxidant-mediated lysis. (mtak.hu)
The cuprous-based coordination polymer successfully delivered a variety of structurally diverse proteins into various cell lines with reserved bioactivities. (bvsalud.org)
Together, these proteins play a role in the uptake of vitamin B12 from food. (medlineplus.gov)

Nuclear uptake and retention of androgen by the pituitary gland of the hamster and the rat. (oregonstate.edu)
Although the number of cells that concentrated the androgen in the pars intermedia and pars nervosa was small, this finding may be related to recent physiologic data that suggest that the gonadal steroids may play a role in regulating water retention and natriuresis. (oregonstate.edu)

1]MIBG uptake by UVW glioma cells transfected with the NAT gene whose expression is driven by the human telomerase RNA (hTR) promoter (70% the uptake achieved by the strong viral promoter). (gla.ac.uk)
Mutations in the AMN gene prevent cubilin from attaching to the cells in the small intestine and kidneys. (medlineplus.gov)
Other CUBN gene mutations affect cubilin's function only in the small intestine, impairing uptake of vitamin B12 into the intestinal cells. (medlineplus.gov)
which means both copies of the gene in each cell have mutations. (medlineplus.gov)
In sickle cell anemia, a point mutation on the β-globin gene results in glutamic acid substituting for valine at position 6 of the amino acid sequence. (medscape.com)
[2] In the United States, 1 in 12 African Americans carries the sickle cell gene and 1 in 375 has sickle cell anemia. (medscape.com)
[3] It is estimated that approximately 72,000 Americans are homozygous for the sickle cell gene (i.e. the SS genotype) and have SCD, [4] and 2 million are heterozygous carriers (i.e. the AS genotype) and therefore have sickle cell trait. (medscape.com)
With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)
A very natural place to study gene detecting and metabolizing lactose, it is known that the overall regulation is in the metabolism of the cell, and then specifically in effect of expressing the lac genes in vain is a drop in the growth rate the regulation of genes that code for enzymes and transporter of as much as 5% [1,6]. (lu.se)

We wondered whether hemoglobin derived from extravasated RBC deposits heme-derived iron into the tumor, which could modulate the sensitivity of cancer cells to oxidant-mediated injury. (mtak.hu)
This protocol provides the details and methods we used for other researchers to screen small molecule libraries for actives that modulate the uptake of fluorescently labelled PFFs uptake in NPCs. (zenodo.org)

Interestingly, the AHA2 proton pump and the FRO2 reductase, both of which work in concert with IRT1 in the acidification-reduction-transport strategy of iron uptake, were part of this interactome. (nih.gov)

Overall, we propose that a dedicated iron-acquisition protein complex exists at the cell surface of Arabidopsis root epidermal cells to optimize iron uptake. (nih.gov)

As circulating PMPs-levels were not altered in Gas6-/- mice compared to Gas6+/+ mice, we hypothesize that the Gas6-mediated uptake is not a means to clear the bulk of circulating PMPs but may serve to phagocytose PMPs locally generated at sites of platelet activation and as a way to affect endothelial responses. (lu.se)

Neonatal Isoflurane Exposure in Rats Impairs Short-Term Memory, Cell Viability, and Glutamate Uptake in Slices of the Frontal Cerebral Cortex, But Not the Hippocampus, in Adulthood. (iasp-pain.org)
We collected worms from contaminated soil after 1, 3, 7, 14, and 28 days of exposure ( Figure 1 , panel A). PMCA results showed that worms exposed to prions take up PrP Sc and efficiently sustain prion replication at all exposure times tested ( Figure 1 , panel B). We observed no PrP Sc uptake in any worms exposed to control soil. (cdc.gov)
A brief exposure (1 h) of 51Cr-radiolabeled breast cancer cells (BT-20) but not colon cancer cells (Caco-2) to hemin (10 microM) or FeSO4 (10 microM) significantly enhances cytotoxicity mediated by 0.5 mM hydrogen peroxide (H2O2). (mtak.hu)
Our results suggested that an exposure to infections in early life prevents allergy through the alteration of the BM cells fate that is to induce the differentiation of BM cells into inhibitory DCs such as NO-producing DCs. (elsevierpure.com)
Several covariates--age, sex, combined HIV exposure category, CD4 cell counts, antiretroviral treatment and AIDS-defining illnesses--were included in the parametric model to predict factors associated with future mortality. (who.int)
The amount of cell lysis increased proportionally to the exposure time to the materials studied. (bvsalud.org)

It was shown, that apoprotein E 139-158 peptide increases liposomal binding followed by receptor-mediated endocytosis by cells PC12. (msk.ru)

We have developed a human Aβ_{1-42} analogue (Aβ^{pH}) that exhibits green fluorescence upon internalization into the acidic organelles of cells but is non-fluorescent at physiological pH. (ucl.ac.uk)

We show that live Wolbachia cells are taken up by host cells at a much faster rate than dead Wolbachia cells, indicating that Wolbachia bacteria play a role in their own uptake and that Wolbachia colonization is not just a passive process. (geneconvenevi.org)
In case of Gram-positive bacteria cadmium uptake decreased by 5-10% after cells were inhibited by contacting with 1M sodium azide for 45 min. (njit.edu)
In the case of Gram-negative bacteria, uptake decreased by 4-5% under same conditions. (njit.edu)
Although cadmium uptake decreased by 4-5% after inhibition of cells with sodium azide, there was no significant increase in cadmium uptake by Gram-negative bacteria after nutrient addition. (njit.edu)

In the presence of serum, however, the majority of B cells bound MBP in a complement-dependent manner, and almost half of the B cells became engaged in presentation of MBP85-99. (regionh.dk)

We also show that the host actin cytoskeleton must be intact for this to occur and that drugs that disrupt the actin cytoskeleton effectively abrogate Wolbachia uptake. (geneconvenevi.org)

Lipid droplets (colored) in a fat cell that lacks EHD2. (azolifesciences.com)
Thus, she could identify that the uptake of fatty acids was higher in cells that lack EHD2 and that the lipid droplets, or intracellular accumulations of fat, were larger in such cells. (azolifesciences.com)
At present, her focus is specifically on analyzing the transport of fatty acids within the cell and the formation of lipid droplets. (azolifesciences.com)

Failure of the immune system to differentiate between apoptotic tumour cells and cells rendered apoptotic as part of homeostasis prevents a successful response being mounted against tumours. (soton.ac.uk)

The inventors' work established that CXCL16, a membranebound scavenger receptor, influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides. (fda.gov)

Thiourea Dendrimer Achieves Excellent Cytosolic Protein Delivery via Enhanced Cell Uptake and Endosome Escape. (bvsalud.org)

Even though complement receptor 1 (CR1, CD35) and CR2 (CD21) both contributed to binding of MBP to B cells, only CR2 was important for the subsequent presentation of MBP85-99. (regionh.dk)

This process occurs when water molecules bind to the cellulose matrix of the cell walls. (sekimo.com)

The vertebrate reticuloendothelial system (RES) functions to remove potentially damaging macromolecules, such as excess hormones, immune-peptides and -complexes, bacterial-endotoxins, microorganisms and tumor cells. (amrita.edu)

Water uptake by plant cells depends on osmoregulation, which controls the solute concentration and water potential in the cell. (sekimo.com)

Under several conditions, the sensitivity of tumor cells to oxidant-mediated lysis is inversely proportional to their ferritin content. (mtak.hu)

The aims of this study were to investigate the effect of the presence of chromium in RBCs on oxygen uptake capacity of blood [oxyhaemoglobin saturation (O2-SAT) at specific pO2 values] and to evaluate the suitability of the two commonly used anti-coagulants, heparin and EDTA, for oxygen uptake studies. (strath.ac.uk)
In this study we investigated how vascular TAMs and their ligands may mediate endothelial uptake of PMPs. (lu.se)

In some places, this early extraordinary spring growth was enough to compensate for the reduction of carbon uptake later on in summer. (sonnenseite.com)

Data analogous to those with H2O2-mediated cytotoxicity were obtained in studies of bleomycin-engendered DNA strand breakage and cell damage, i.e., brief treatment of BT-20 cells with both hemin or FeSO4 significantly increases their sensitivity to bleomycin (100 micrograms/ml), whereas treatment followed by 24 h incubation with media alone significantly protects against bleomycin toxicity. (mtak.hu)
Cell uptake studies using HEK-293 cells with the fluorescent cyclic decoy in the presence of lipofectamine 2000 transfection agent indicated that this analogue is taken up by the cells and localizes to the cell nucleus. (ox.ac.uk)

Uptake of AcAc LDL was not observed in the endothelia of arteries (including the coronaries and aorta), veins, or capillaries of the heart, testes, kidney, brain, adipose tissue, and duodenum. (rupress.org)
A 12-kDa cysteine-rich polypeptide hormone secreted by FAT CELLS in the ADIPOSE TISSUE. (bvsalud.org)

2003). At physiological pH, Cr (VI) exists predominantly as the chromate anion and as such can enter cells via non-specific anion channels. (strath.ac.uk)
Possible employment of cell-specific peptide for the specific adsorption and uptake by cells. (msk.ru)
Here, the function of regulation is quite clear: expressing energy and carbon, that a number around 0.2% would be the right genes at the right time will enable the cell to make the expected, and that the difference is more or less specific to the lac most of the resources within its reach, by maximizing the uptake operon [7]. (lu.se)

The main target of this project is to develop computational models and perform a series of experimental validations to identify the role of nanoparticle's mechanical stiffness in the cell uptake. (uga.edu)
MBP-pulsed B cells induced a low frequency of IL-10-producing CD4+ T cells in 3 out of 6 donors, indicating an immunoregulatory role of B cells presenting MBP-derived peptides. (regionh.dk)

An ethyl acetate extract of orange juice did not affect the initial uptake rate of [ 3 H]vinblastine but significantly increased the steady-state uptake, as did cyclosporin A (20 μM), an inhibitor of P-gp. (aspetjournals.org)

We also develop and use live-cell assays to show that {PCs} take up soluble macromolecules in a Dynamin-dependent manner and colloids by a Dynamin-independent pathway. (amrita.edu)

Amnionless helps transfer the cubilin-vitamin B12 complex into the intestinal cell. (medlineplus.gov)

When the extract was separated on a Cosmosil column, the eluate with 70% methanol showed the most potent ability to increase [ 3 H]vinblastine uptake. (aspetjournals.org)
B cells may play both pathogenic and protective roles in T-cell mediated autoimmune diseases such as multiple sclerosis (MS). These functions relate to the ability of B cells to bind and present antigens. (regionh.dk)

In plants, iron uptake from the soil is tightly regulated to ensure optimal growth and development. (nih.gov)

Anatomy30

Organisms10

Chemicals and Drugs153

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes38

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Health Care1

Endothelial cells11

Cellular15

Nanoparticle uptake2

Dendritic cells9

Receptors4

Liposomes4

Liposome uptake1

Mechanism7

Nanoparticles by cells1

Assay4

Carbon dioxide2

Vivo5

Macrophages3

Mechanisms3

Lipoproteins1

Mediates1

Neuronal1

Apoptotic cells4

Membrane12

Improves1

Degradation4

Increased synthesis1

Inhibits1

Antigen6

Vitro5

Induces1

Metabolic2

Interactions4

Proteins5

Retention2

Gene9

Modulate2

Interestingly1

Epidermal1

Mice1

Exposure6

Endocytosis1

Internalization1

Bacteria4

Serum1

Cytoskeleton1

Lipid droplets3

Tumour cells1

Scavenger1

Protein1

Subsequent1

Occurs1

Microorganisms1

Depends1

Tumor cells1

Study2

Reduction1

Studies2

Adipose tissue2

Specific3

Role2

Significantly1

Dependent1

Intestinal1

Ability2

Soil1