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  • assay
  • Using the transfection of the miR-503 gene into U937 cells and Luciferase assay, we confirmed that miR-503 suppressed the expression of CD40, and was a negtive regulator of CD40. (biomedcentral.com)
  • It was found that PCN increased IL-8 release and mRNA expression in Phorbol 12-myristate 13-acetate (PMA) differentiated U937 cells in both a concentration- and time-dependent manner by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • The validation of designed siRNA molecules as to their silencing efficiency is carried out in vitro cell based assay. (recentscientific.com)
  • METHODS
  • Methods and Results -Both lipopolysaccharide (LPS)- and N -formyl-methionyl-leucyl-phenylalanine (FMLP)-stimulated U937 adhesion to HUVECs were studied. (ahajournals.org)
  • OxLDL
  • The oxidation of low-density lipoprotein (LDL) to oxidized LDL (oxLDL) and its subsequent uptake by macrophages to form foam cells are the key process in plaque formation. (canterbury.ac.nz)
  • OxLDL caused a rapid concentration-dependent cell viability loss in U937 cells within 6 hours. (canterbury.ac.nz)
  • The progression of oxLDL-induced cell death was found to be strongly correlated with the intracellular ROS production and intracellular glutathione (GSH) loss. (canterbury.ac.nz)
  • Further investigations using NOX inhibitors apocynin or diphenyleneiodonium (DPI) found that inhibition of NOX prevented oxLDL-induced cell viability loss, ROS production, GSH loss and aconitase activity decrease. (canterbury.ac.nz)
  • 7,8-NP dramatically protected cells from oxLDL-induced cell viability loss, ROS generation and aconitase activity loss. (canterbury.ac.nz)
  • Objective- Foam cell (FC) formation by oxidized low-density lipoprotein (oxLDL) accumulation in macrophages is crucial for development of atherosclerosis. (ahajournals.org)
  • mRNA expression
  • Differentiation of promonocytic cells to monocytes is therefore characterized by decreased SVCT2 mRNA expression that, even prior to the onset of SVCT2 protein downregulation or apparent changes in plasma membrane transport activity, impacts on the mitochondrial accumulation of the vitamin through a decreased Vmax of the transporter. (hindawi.com)
  • It is concluded that PCN induces IL-8 secretion and mRNA expression in PMA-differentiated U937 cells in a concentration- and time- dependent manner. (biomedcentral.com)
  • stimulation
  • After stimulation with phorbol-12 myristate acetate (PMA) for 24h, U937 cells were cultured in the absence or presence of lipopolysaccharide (LPS: 1 and 5 μg ml -1 ) and glucocorticosteroids (budesonide, fluticasone propionate and prednisolone: 10 -11 , 10 -9 and 10 -7 M) for 96h. (hindawi.com)
  • adhesion
  • Cell adhesion to the 38-kD fragment was not inhibited by the 80-kD fragment, by GRGDSPC synthetic peptides, or by a mAb directed to the RGDS-containing domain of Fn. (rupress.org)
  • Expression of the adhesion molecules CD11a, CD11b, CD11c, very late antigen-4 (VLA-4), and L-selectin, as assessed by flow cytometry, was increased in the stimulated U937 cells, and AT resulted in significant reduction in the expression of CD11b and VLA-4. (ahajournals.org)
  • 3 These adhesion molecules include E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) on ECs and members of the β-2 integrin family (CD11a, CD11b, and CD11c/18) and the β-1 integrin VLA-4 (very late antigen-4 [CD49d/ on monocytes. (ahajournals.org)
  • 10 Faruqi et al 11 showed that AT enrichment of ECs inhibited agonist-induced adhesion of U937 cells to HUVECs. (ahajournals.org)
  • Growth
  • Early studies have shown that PCN causes multiple effects on human cells, such as inhibition of cell respiration, ciliary function, epidermal cell growth, and prostacyclin release. (biomedcentral.com)
  • Methodology/Principal Findings Icariside II blocked the growth U937 cells in a dose- and time-dependent manner. (harvard.edu)
  • cleavage
  • this cleavage leads to the binding of the resulting C3 fragments to the cell membrane through reaction of membrane acceptors with the carbonyl group of C3 revealed after disruption of the intramolecular thioester bond. (biochemj.org)
  • In U937 cells, DATS-induced apoptosis was correlated with down-regulation of Bcl-2, XIAP, and cIAP-1 protein levels, cleavage of Bid proteins, activation of caspases, and collapse of mitochondrial membrane potential. (biomedcentral.com)
  • concentrations
  • Under these conditions, cells accumulate high concentrations of the vitamin that can be further transported within specific organelles in which these transporters are also expressed [ 4 ]. (hindawi.com)
  • The results were further confirmed by the observation that p38, ERK1/2 and NF-κB inhibitors inhibited PCN-induced NF-κB activation and attenuated PCN-induced IL-8 expression in U937 cells as a function of their concentrations. (biomedcentral.com)
  • survival
  • Although each pathway is initially mediated by different mechanisms, they share a common final phase of apoptosis, consisting of the activation of the executioner caspases and dismantling of substrates critical for cell survival ( 7 ). (aacrjournals.org)
  • inhibitor
  • Icariside II also enhanced the expression of protein tyrosine phosphatase (PTP) SH2 domain-containing phosphatase (SHP)-1, and the addition of sodium pervanadate (a PTP inhibitor) prevented Icariside II-induced apoptosis as well as STAT3 inactivation in STAT3 positive U937 cells. (harvard.edu)
  • transfection
  • By stable U-937 cell transfection with a GRK2-antisense cDNA, we obtained D5 and A2 cell clones exhibiting a reduction in GRK2 expression and an H3 clone with no significant difference in GRK2 expression from control cells. (conicet.gov.ar)
  • results
  • Taken together, these results strongly suggest that H2O2 is entirely responsible for the ascorbate-induced U937 cell killing. (aspetjournals.org)
  • These results indicate that U937 cells interact with two sites of Fn, the RGDS-containing region, and the IIICS region. (rupress.org)
  • Our results disclose a novel action of celastrol-- causing cell cycle arrest at G0/G1 phase based upon thiol-related HSP90 inhibition. (biomedcentral.com)
  • Overall, these results indicate a direct correlation between the expression of GRK2 and the desensitization of natively expressed H2 receptors in U-937 cells, suggesting that GRK2 plays a major role in the regulation of these receptors' response. (conicet.gov.ar)
  • secretion
  • Secretion of complement component C3 by U937 cells was studied. (biochemj.org)
  • Secretion of C3, in the presence of 10 ng of phorbol 12-myristate 13-acetate/ml, is 120-140 ng/10(6) cells per 24 h on the third day after addition of the activator. (biochemj.org)
  • 4 Hypoxia-induced secretion of IL-8 from FCs may lead to the recruitment of smooth muscle, vascular endothelial, and T cells into the atherosclerotic plaques and thus to plaque progression. (ahajournals.org)
  • mitochondrial
  • Interestingly, under the same conditions, these cells accumulated lower amounts of the vitamin in their mitochondria, with an ensuing reduced response to external stimuli sensitive to the mitochondrial fraction of AA. (hindawi.com)
  • Lysosomal-mitochondrial cross-talk during cell death. (semanticscholar.org)
  • dose
  • In the present study, we applied ionizing radiation with a dose of either 0.1 Gy or 5 Gy to irradiate U937 cells to confirm CD40 as a miR-503 target, which was identified using a bioimformatics tool. (biomedcentral.com)
  • In high dose (5 Gy) ionizing-irradiated U937 cells, expression of miR-503 was up regulated while the expression of CD40 gene was down regulated. (biomedcentral.com)
  • regulation
  • Further investigations on the regulation of cytokine production and release by TPA-differentiated U937 cells revealed that TNF-alpha and IL-1 beta synthesis was not influenced by exogenously added rhTNF-alpha or PGE2, whereas rh gamma-IFN specifically enhanced the IL-1 beta production. (uni-regensburg.de)
  • bind
  • U937 did not bind to a 58-kD fragment derived from the B chain (which lacks IIICS) and has the Hep II site. (rupress.org)
  • The proteolysis appears to be fairly specific to C3, as C4, which also possesses an intramolecular thioester bond, is not cleaved and does not bind to the cells. (biochemj.org)
  • Effects
  • The extract showed no significant effects on the superoxide production of both neutrophils and U937 cells, as measured by chemiluminescence. (shroomery.org)
  • However, this agent's effects on the cell cycle are rarely investigated. (biomedcentral.com)
  • The observed effects of celastrol on the U937 cell cycle were thiol-related, firstly because the effects could be countered by pre-loading thiol-containing agents and secondly because celastrol and thiol-containing agents could react with each other to form new compounds. (biomedcentral.com)
  • Anti-tumor effects can be achieved by cell death and/or cell cycle arrest. (biomedcentral.com)
  • Until now, most reports attributed celastrol's anti-tumor effects on its death-triggering action, leaving celastrol's effects on cell cycle almost unexplored. (biomedcentral.com)
  • 7 8 In addition to decreasing the oxidative susceptibility of LDL, AT has been shown to have direct antiatherogenic effects on cells. (ahajournals.org)
  • progression
  • Progression of the initial fatty streak to an advanced atherosclerotic plaque is characterized by the development of a necrotic core region containing cholesterol and dead cells. (canterbury.ac.nz)
  • mechanism
  • However, the molecular mechanism involved in cell death induced by evodiamine treatment remains poorly understood. (aacrjournals.org)
  • The aims of the present study were to investigate the participation of GRK2 in the desensitization mechanism of the H2 receptor in U-937 cells by reducing GRK2 levels through antisense technology and to evaluate the differentiating capacity of cells expressing lower GRK2 level, stimulated by H2 agonists. (conicet.gov.ar)
  • activity
  • Preliminary evidence for a cell-associated proteolytic activity specific for C3 is given, as well as for a covalent-like binding of C3 fragments to the cell membranes. (biochemj.org)
  • Cell cycle arrest was accompanied by inhibition of HSP90 ATPase activity and elevation in HSP70 levels (a biochemical hallmark of HSP90 inhibition), a reduction in Cyclin D1, Cdk4 and Cdk6 levels, and a disruption of the HSP90/Cdc37/Cdk4 complex. (biomedcentral.com)