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  • Acetylation
  • TSA-induced increases in acetylation of IRS-1 were concomitant with increases in tyrosine phosphorylation in response to insulin. (biomedcentral.com)
  • Overall, our study suggests that S -glutathionylation and phosphorylation of IKKα plays an important role in histone acetylation on proinflammatory gene promoters and NF-κB-mediated abnormal and sustained lung inflammation in pathogenesis of chronic inflammatory lung diseases. (physiology.org)
  • It is well known that CS exposure causes inflammation in the lung by activation of redox-sensitive NF-κB via phosphorylation/acetylation of its subunit, RelA/p65 ( 11 , 39 , 54 ). (physiology.org)
  • phosphorylate
  • IKKβ is shown to phosphorylate NH 2 -terminal serines on IκBα, whereas IKKα regulates non-canonical pathway of NF-κB in response to a variety of inflammatory stimuli ( 33 , 43 , 53 ). (physiology.org)
  • phosphate
  • Phosphorylation allows cells to accumulate sugars because the phosphate group prevents the molecules from diffusing back across their transporter. (wikipedia.org)
  • Researcher D. G. Walker of the University of Birmingham determined the presence of two specific enzymes in adult guinea pig liver, both of which catalyze the phosphorylation of glucose to glucose 6 phosphate. (wikipedia.org)
  • The phosphorylation of glucose can be enhanced by the binding of Fructose-6-phosphate, and lessened by the binding fructose-1-phosphate. (wikipedia.org)
  • histidine
  • Due to the chemical lability of these phosphorylated residues, and in marked contrast to Ser, Thr and Tyr phosphorylation, the analysis of phosphorylated Histidine using standard biochemical and mass spectrometric approaches is much more challenging and special procedures and separation techniques are required for their preservation alongside classical Ser, Thr and Tyr phosphorylation. (wikipedia.org)
  • inhibition
  • Maspin, a 42 kDa tumor suppressor protein, belongs to the serpin (serine protease inhibitors) superfamily, however, its mechanism of action does not depend on protease inhibition. (usp.br)
  • pathway
  • Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway. (nih.gov)
  • Epidermal growth factor receptor and PKC inhibitors inhibited basal levels of S(1356) phosphorylation in SCC, suggesting that cells use intrinsic mechanisms to activate the EGF signaling pathway to induce β4 phosphorylation. (biomedsearch.com)
  • vitro
  • In lymphoblastoid cell cultures, Syk tyrosine kinases have been found in LMP2A immunoprecipitates following in vitro kinase reactions followed by Syk antibody reimunnoprecipitation. (wikipedia.org)
  • Coomassie staining), mitochondrial membrane potential (by JC-1), DNA damage (comet assay), oxidative damage (malondialdehyde (MDA) production), in vitro fertilization and protein phosphorylation (immunodetection), and erythrocyte acetylcholinesterase (AChE) activity. (osti.gov)
  • To assess the mechanisms by which SCC increases β4 phosphorylation, we performed in vitro analyses. (biomedsearch.com)
  • antibody
  • p-Ser Antibody (4A3) recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts. (scbt.com)
  • The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. (abcam.cn)
  • Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. (abcam.cn)
  • peptide
  • Synthetic peptide derived from the region of FAK that contains serine 910. (abcam.cn)
  • The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 910. (abcam.cn)
  • contrast
  • In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. (abcam.cn)
  • In contrast, when stimulated with TCR agonists together with IL-6 and TGF-β, IRAK-1 −/− CD4 Th cells exhibit attenuated STAT3 Ser727 phosphorylation and reduced expression of IL-17 and RORγt compared with wild-type cells. (jimmunol.org)