• Here, we report that membrane-associated tyrosine kinase and protein-tyrosine phosphatase (PTPase) activities regulate tER assembly by stabilizing (PTPase) or destabilizing (tyrosine kinase) p97 association with membranes. (pnas.org)
  • Tyrosine phosphorylation of p97 in T cells has been observed as a consequence of p60 fyn overexpression as well as treatment of these cells with protein-tyrosine phosphatase (PTPase) inhibitors ( 14 ). (pnas.org)
  • Moreover, p97 is the major substrate of the protein-tyrosine phosphatase PTPH1 ( 16 ), and overexpression of PTPH1 in NIH 3T3 cells leads to p97 dephosphorylation coinciding with an inhibition of cell-cycle progression and the accumulation of cells throughout the cell cycle ( 16 ). (pnas.org)
  • We demonstrated that protein tyrosine phosphatase non-receptor 22 (PTPN22), variants in which are associated with chronic inflammatory disorders, dephosphorylates NLRP3 upon inflammasome induction, allowing efficient NLRP3 activation and subsequent IL-1β release. (uzh.ch)
  • HRAS-Tyr(137) phosphorylation enhanced HRAS signaling capacity in cells, however, as reflected by a 4-fold increase in the association of phosphorylated HRAS(G12V) with its effector protein RAF proto-oncogene serine/threonine protein kinase 1 (RAF1). (sigmaaldrich.com)
  • These data suggest that RAS phosphorylation at Tyr(137) allosterically alters protein conformation and effector binding, providing a mechanism for effector-initiated modulation of RAS signaling. (sigmaaldrich.com)
  • Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: identification of a c-Src/Cav-1/Grb7 signaling cassette. (semanticscholar.org)
  • This has revealed a tyrosine phosphorylation signature that characterizes the basal phenotype, and identified a prominent Src family kinase (SFK) signaling network in basal breast cancer cells. (aacrjournals.org)
  • Incubation with the PTPase inhibitor bpV(phen) inhibited tER assembly coincident with the enhanced tyrosine phosphorylation of endogenous p97 and its release from membranes. (pnas.org)
  • By contrast, the tyrosine kinase inhibitor, genistein, promoted tER formation and prevented p97 dissociation from membranes while increasing p97 association with the t-SNARE syntaxin 5. (pnas.org)
  • In addition, in activated B lymphocytes, p97 becomes tyrosine phosphorylated after treatment with the PTPase inhibitor, sodium vanadate ( 15 ). (pnas.org)
  • Tyrosine phosphorylation is a key step in signal transduction and the regulation of enzymatic activity. (wikipedia.org)
  • Here, we have described a mechanism of NLRP3 inflammasome regulation by tyrosine phosphorylation of NLRP3 at Tyr861. (uzh.ch)
  • In summary, the present investigation has provided new evidence showing that sperm capacitated in vitro in the presence of EOV display an enhanced expression of tyrosine phosphorylation compared to sperm incubated in capacitating medium alone. (queensu.ca)
  • In 8 of 18 primary androgen-independent prostate tumor samples, tyrosine-phosphorylated AR protein was detected and correlated with the detection of tyrosine-phosphorylated Ack1. (pnas.org)
  • Tyrosine phosphorylation of RAS by ABL allosterically enhances effector binding. (sigmaaldrich.com)
  • In light of findings reported in previous studies, we hypothesized that estrus stage-specific oviductin (EOV) enhances tyrosine phosphorylation. (queensu.ca)
  • Ack1 knockdown inhibited heregulin-dependent AR tyrosine phosphorylation, AR reporter activity, androgen-stimulated gene expression, and AR recruitment. (pnas.org)
  • Although the role of tyrosine phosphorylation in this network is clear, only a limited number of insulin-induced tyrosine phosphorylation sites have been identified. (diabetesjournals.org)
  • Insulin treatment caused a change of at least 1.3-fold in tyrosine phosphorylation on 89 of these sites. (diabetesjournals.org)
  • PDGF-induced activation of phospholipase C activity is abrogated in cells expressing PLCγ mutated in the tyrosine phosphorylation sites. (wikipedia.org)
  • Adenovirus-mediated overexpression of a c-Cbl mutant that ablates the major tyrosine phosphorylation sites attenuated the Akt and the mammalian target of rapamycin activation and decreased the proliferation and migration of smooth muscle cells in response to PDGF-BB or fibroblast growth factor. (ahajournals.org)
  • When comparing MCF-10A and MCF-10A/SrcY527F cells, phosphorylation sites exhibiting a difference in mean spectral counts of at least 1.5 in two independent experiments were considered as differentially phosphorylated. (aacrjournals.org)
  • Mutational analysis showed that Tyr{sup 174}, Tyr{sup 183} and Tyr{sup 426} of chicken 3BP2 are the major phosphorylation sites by Syk and the SH2 domain of 3BP2 is critical for tyrosine phosphorylation. (osti.gov)
  • Tyr183 and Tyr426 in chicken 3BP2 are the major phosphorylation sites by Syk. (osti.gov)
  • Although tyrosine phosphorylation has been recognised as an important factor in capacitation, the precise relationship between the phosphorylation status of mammalian spermatozoa and their capacity for fertilisation has not yet been elucidated. (biologists.org)
  • Importantly, in balloon-injured rat carotid arteries, local delivery of c-Cbl-m reduced the phosphorylation of Akt and the mammalian target of rapamycin, inhibited the migration and proliferation of smooth muscle cells, and prevented neointimal hyperplasia. (ahajournals.org)
  • Tyrosine phosphorylation is closely associated with cell proliferation. (sciencemag.org)
  • We and others have recently shown involvement of activated Cdc42-associated tyrosine kinase Ack1 in advanced human prostate cancer. (pnas.org)
  • Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophages is mediated by CD14. (jimmunol.org)
  • Approximately half of the tyrosine kinase complement of the human "kinome" is implicated in human cancers ( 4 ), and provides important targets for cancer treatment, as well as biomarkers for patient stratification. (aacrjournals.org)
  • In the present study, we examined the role of c-Cbl tyrosine phosphorylation in vascular injury. (ahajournals.org)
  • To determine whether connexin43, a major gap junction protein expressed in fibroblasts, is a target for the v-src protein tyrosine kinase activity, we examined the phosphorylation state of connexin43 in cells expressing variants of src. (biomedsearch.com)
  • We have also examined junctional permeability in cells expressing src variants and find that loss of cell-to-cell communication correlates with tyrosine phosphorylation of connexin43. (biomedsearch.com)
  • 23415227 ). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. (uniprot.org)
  • For example, in their network generated from data mining and in cultured cells, tyrosine phosphorylation decreased activation of Polo-like kinase 1 (PLK1), a serine/threonine kinase that promotes chromosome separation during anaphase and is often excessively abundant in cancers. (sciencemag.org)