CollagenProteinsFibrinogenProtein fibronectinReceptorFibroblastsCollagensFibrilsGlycoproteinAdhesionGeneIsoformsReceptorsFibrinProteolyticFragmentDomainsCytoplasmicGeneticSequenceBindTumorAntibodiesEpithelialDomainDifferencesMechanismsMeasurementsSurfacesMembraneCellsCellMajorSimilarExpressionVascularSystemSurfaceSpecificStudy
Collagen20
- Fibronectin type II domain is a collagen-binding protein domain. (wikipedia.org)
- Fibronectin is a multi-domain glycoprotein, found in a soluble form in plasma, and in an insoluble form in loose connective tissue and basement membranes, that binds cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. (wikipedia.org)
- Type II domain is approximately sixty amino acids long, contains four conserved cysteines involved in disulfide bonds and is part of the collagen-binding region of fibronectin. (wikipedia.org)
- These fragments are useful tools for mapping regions within the fibronectin molecule, which are responsible for specific binding to collagen and for other biologically relevant functions. (sigmaaldrich.com)
- Conversely, both fibronectin and collagen XVIII are increased in scleroderma skin, suggesting their involvement in the pathogenesis of bullous scleroderma. (cdlib.org)
- Although knockdown of circRNA_33702 or hsa_circ_0026331 was shown to relieve the TGF- β 1-induced expression of collagen I, collagen III, and fibronectin, overexpression of circRNA_33702 was found to exert an inhibitory effect on the expression of the same genes. (nih.gov)
- MiR-29b-3p mimic was shown to inhibit the TGF- β 1-induced expression of collagen I, collagen III, and fibronectin. (nih.gov)
- Notably, knockdown of circRNA_33702 was found to attenuate the expression of collagen I, collagen III, and fibronectin by inhibiting the expression of WISP1, and the observed inhibitory effect can be reversed by miR-29b-3p inhibitor. (nih.gov)
- Stultz CM., Insight into the Degradation of Type-I Collagen Fibrils by MMP-8. (mit.edu)
- The mesohyl includes a noncellular colloidal mesoglea with embedded collagen fibers, spicules and various cells, being as such a type of mesenchyme. (pearltrees.com)
- Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
- In canine epidermolysis bullosa acquisita, the immunoglobulin G (IgG) autoantibodies also target the type VII collagen noncollagenous (NC1) domain, which shares greater than 80% homology in amino acid sequence with the human NC1 domain. (medscape.com)
- A subset of patients have variations in which either IgG autoantibodies bind the central triple-helical collagenous (NC2) domain of type VII collagen, or immunoglobulin A (IgA), rather than IgG, targets type VII collagen. (medscape.com)
- [ 3 ] IgG autoantibodies specific for anchoring fibrils (type VII collagen) of the skin basement membrane have a heterogeneous subclass and light-chain composition, and their complement-activating capacities do not correlate with the inflammatory phenotype. (medscape.com)
- Initial studies demonstrated that rabbit antimouse type VII collagen antibodies, as well as rabbit antibodies raised against human recombinant protein type VII collagen, were able to induce blisters in mice. (medscape.com)
- [ 7 ] The failure of rabbit antimouse type VII collagen antibodies to induce blisters in C5-deficient mice supports a role for complement activation in the disease pathogenesis. (medscape.com)
- Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)
- Immunization of type VII collagen in athymic nude SJL mice did not induce an autoimmune response, whereas the repletion of T cells from type VII collagen-immunized wild-type mice to the thymic mice showed autoantibody production and resulted in a blistering disease phenotype, supporting the role of T cells in the induction of epidermolysis bullosa acquisita. (medscape.com)
- Epidermolysis bullosa acquisita (EBA) is an autoimmune disease, manifested by autoantibodies that target type VII collagen, the major protein of anchoring fibrils and the one that connects the epithelial basement membrane to the dermis. (medscape.com)
- Autoantibodies specific for type VII collagen alter the dermoepidermal junctional adhesion and lead to dermoepidermal separation. (medscape.com)
Proteins9
- Fibronectin type II domain is part of the extracellular portions of EphA2 receptor proteins. (wikipedia.org)
- While laminin and fibronectin are widely used ECM proteins, the one that your cell needs should be determined by test experiments. (sigmaaldrich.com)
- Bonds between fibronectin and fibronectin-binding proteins on Staphylococcus aureus and Lactococcus lactis. (duke.edu)
- Bacterial cell-wall-associated fibronectin binding proteins A and B (FnBPA and FnBPB) form bonds with host fibronectin. (duke.edu)
- Type III modules are present in both extracellular and intracellular proteins. (embl.de)
- Each domain consists of two antiparallel beta sheets and is folded topologically identically to single fibronectin type III domains from the extracellular matrix proteins tenascin and fibronectin. (embl.de)
- Endosialin likely interacts with a network of extracellular and hypoxia activated proteins in sarcomas and other tumor types. (hindawi.com)
- The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. (microbiologyresearch.org)
- Formation of H. pylori cell surface-bound plasmin may be important to provide a powerful proteolytic mechanism for gastric tissue penetration in type B gastritis and peptic ulcer disease, since plasmin degrades not only fibrin but also extracellular matrix proteins such as various collagens and fibronectin. (lu.se)
Fibrinogen2
- CD41/CD61 is expressed on platelets and megakaryocytes, and plays a role in platelet activation and aggregation through interaction with fibrinogen, fibronectin, vWF, and other RGD-containing adhesion molecules. (biolegend.com)
- Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. (microbiologyresearch.org)
Protein fibronectin2
- Using fluorescence resonance energy transfer (FRET), we detected major changes in the conformation of a constituent ECM protein , fibronectin (Fn), as cells fabricated a thick three-dimensional (3D) matrix over the course of three days. (rsc.org)
- One of three types of internal repeat within the plasma protein, fibronectin. (embl.de)
Receptor5
- and Yamada, Kenneth M., "Differences in the Biosynthesis and Localization of the Fibronectin Receptor in Normal and Transformed Cultured Human Cells" (1990). (howard.edu)
- The eighth fibronectin type III domain of protein tyrosine phosphatase receptor J influences the formation of protein complexes and cell localization. (unina.it)
- Regulation of receptor-type phosphatases can involve the formation of higher-order structures, but the exact role played in this process by protein domains is not well understood. (unina.it)
- In this study we show the formation of different higher-order structures of the receptor-type phosphatase PTPRJ, detected in HEK293A cells transfected with different PTPRJ expression constructs. (unina.it)
- Endosialin is classified as a C-type lectin-like protein and shares both sequence and structural homology with thrombomodulin (CD141) and complement receptor C1qRp (CD93) [ 2 ]. (hindawi.com)
Fibroblasts2
- It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. (nature.com)
- Studies have identified pericytes and reactive fibroblasts, two essential cell types required for tumor survival and growth, as the major sites of expression of endosialin. (hindawi.com)
Collagens2
- Our aim is to report a rare case of bullous scleroderma, studying the presence of fibronectin and collagens type I, III and XVIII in sclerodermic skin. (cdlib.org)
- Indirect immunofluorescence measured by laser confocal microscopy allows quantitative determination of fibronectin and collagens type I, III and XVIII. (cdlib.org)
Fibrils1
- These data provide the first evidence that matrix maturation occurs and that aging is associated with increased stretching of fibronectin fibrils, which leads to at least partial unfolding of the secondary structure of individual protein modules. (rsc.org)
Glycoprotein2
- CD61, also known as integrin ß3 and glycoprotein IIIa (gpIIIa), is a 90 kD type I integral transmembrane glycoprotein. (biolegend.com)
- Fibronectin is a dimeric glycoprotein composed of disulfide-linked subunits with a molecular weight of 220-250kDa each. (embl.de)
Adhesion2
- To demonstrate differences in human lens epithelial cell adhesion to different intraocular lens biomaterials in vitro and to determine whether these differences can be influenced by coating the intraocular lens surface with commercially available fibronectin. (arvojournals.org)
- We report the crystal structure of two adjacent fibronectin type III repeats from the Drosophila neural cell adhesion molecule neuroglian. (embl.de)
Gene1
- Fibronectin gene polymorphisms are associated with the development of B-cell lymphoma in type II mixed cryoglobulinemia. (cdc.gov)
Isoforms1
- 2022). Fibronectin isoforms in skeletal development and associated disorders. (mcgill.ca)
Receptors1
- Belongs to the type I cytokine family of receptors. (lu.se)
Fibrin2
- After deepithelialization, the graft was placed with a fibrin-fibronectin system at the maximum root coverage level, and the flap coronally advanced and sutured. (hindawi.com)
- Aim of this case report was to describe a modified bilaminar technique, with a reduced mesiodistal dimension of the CTG and bonded to the radicular dentin by means of a fibrin-fibronectin system. (hindawi.com)
Proteolytic1
- Fibronectin fragments are obtained from plasma fibronectin by using proteolytic enzymes. (sigmaaldrich.com)
Fragment2
- Right here, we examined whether or not function-blocking mAbs directed towards α5β1 can work together with the integrin after it varieties a posh with an RGD-containing fragment of fibronectin. (holliseden.com)
- We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). (embl.de)
Domains4
- The major part of the sequence of fibronectin consists of the repetition of three types of domains, which are called type I, II, and III. (wikipedia.org)
- Type II domains occur two times in fibronectin. (wikipedia.org)
- The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. (nih.gov)
- Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2.0 A. (embl.de)
Cytoplasmic1
- 1][4] Most types of intermediate filaments are cytoplasmic, but one type, the lamins, are nuclear. (pearltrees.com)
Genetic2
- For type IV, a prenatal diagnosis by means of polymorphic restriction genetic studies is possible. (medscape.com)
- Genetic insights into the disease mechanisms of type II mixed cryoglobulinemia induced by hepatitis C virus. (cdc.gov)
Sequence2
Bind3
- A brand new mannequin of the α5β1-fibronectin complicated helps our speculation that the epitopes of mAbs that fail to bind to the ligand-occupied state lie inside, or very near, the integrin-fibronectin interface. (holliseden.com)
- Another is the ability of M protein to bind a human protein called fibronectin (Fn). (lu.se)
- In the third paper we investigated the M protein's ability to bind fibronectin. (lu.se)
Tumor3
- So as to generate an antibody directed enzyme prodrug remedy, right here we designed a chimeric protein by fusing the F8 antibody that acknowledges the EDA of fibronectin (expressed on the tumor neovasculature) and an developed variant of the ROS-generating enzyme D-amino acid oxidase (DAAO). (holliseden.com)
- It is now accepted that the formation of solid tumors requires the proliferation of stromal cells to support cancer cell growth, invasion, and metastasis [ 5 ] and that the stromal cell compartment comprises a heterogeneous mix of cell types responsible for the formation of blood vessels as well as supporting a microenvironment commensurate with tumor growth and proliferation. (hindawi.com)
- Taken together, these data suggest that endosialin plays a role in forming and/or stabilizing the tumor ECM and thus the tumor microenvironment, in addition to its roles in neovascularization and cross-talk between various cell types. (hindawi.com)
Antibodies2
- To acquire correct information utilizing anti-VWF antibodies, it's needed to pay attention to the opportunity of cross-reactivity with fibronectin. (holliseden.com)
- We found that the binding of antibodies from the blood of donors who had recently recovered from a severe GAS infection could greatly enhance this fibronectin binding. (lu.se)
Epithelial3
- The main outcome measure was the mean number of lens epithelial cells attached to each lens type after 24 hours of incubation. (arvojournals.org)
- Coating the lenses with fibronectin caused a significant increase in attachment of lens epithelial cells for all three lens types. (arvojournals.org)
- There was a significant difference in the degree of lens epithelial cell attachment to the various types of intraocular lenses in vitro. (arvojournals.org)
Domain4
- However, other PTPRJ complexes, dependent on the formation of disulfide bonds, are detected by treatment with the oxidant agent H(2)O(2) or by a mutation Asp872Cys, located in the eighth fibronectin type III domain of PTPRJ. (unina.it)
- A deletion in the eighth fibronectin domain of PTPRJ impairs its dimerization in the plasma membrane and increases the formation of PTPRJ complexes dependent on disulfide bonds that remain trapped in the cytoplasm. (unina.it)
- Therefore, the intact structure of the eighth fibronectin domain of PTPRJ is critical for its localization in plasma membrane and biological function. (unina.it)
- 2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region. (embl.de)
Differences2
- Differences in the Biosynthesis and Localization of the Fibronectin Re" by Steven K. Akiyama, Hannu Larjava et al. (howard.edu)
- Cell attachment was more prominent in the acrylic lenses, but the fibronectin coating negated differences in lens type and caused a significant increase in cell attachment across all groups. (arvojournals.org)
Mechanisms1
- Surprisingly, the mechanisms of fibronectin assembly remain largely unknown. (jefferson.edu)
Measurements2
- These measurements demonstrate that fibronectin-binding adhesins FnBPA and FnBPB are necessary and sufficient for the binding of S. aureus to prosthetic devices that are coated with host fibronectin. (duke.edu)
- For type VI, measurements of lysyl hydroxylase (LH) in the amniotic fluid can be used to predict the outcome of pregnancy. (medscape.com)
Surfaces1
- Methods and Results: Biofilm formation on plastic and cFn-coated surfaces by the wild-type M1 and M41 strains, and their isogenid sc/1-inactivated mutants, we compared spectrophotometrically following crystal violet staining. (cdc.gov)
Membrane1
- The deletion mutant maintains the catalytic activity but is unable to carry out inhibition of proliferation on HeLa cells, achieved by the wild type form, since it does not reach the plasma membrane. (unina.it)
Cells3
Cell5
- Optimum conditions for attachment are dependent on cell type and application. (sigmaaldrich.com)
- Fibronectin and TGF-beta are important supplements for growing, maintaining, and differentiating several stem and immune cell types. (rndsystems.com)
- Endothelial cell attachment was supported within the hollow core of the tube, and seen by S.E.M. In this study the development of the proposed fibronectin material is considered, regarding its potential roles in skin replacement therapy and vascular grafting. (ucl.ac.uk)
- Role of endothelial cell-substrate contact area and fibronectin-recept" by Jeffrey S. Burmeister, Valerie Z. McKinney et al. (pacific.edu)
- Scatchard plot analysis revealed one binding mechanism with a Kd value of 7 × 107 M. Conversion of H. pylori cell-bound plasminogen to plasmin in the presence of a tissue-type plasminogen activator was demonstrated by digestion of the chromogenic substrate S-2251. (lu.se)
Major1
- An SDS PAGE gel is run and one major fibronectin band of 120kDa is compared to past lots to ensure consistent quality. (sigmaaldrich.com)
Similar2
- By contrast, ectopic expression of FnBPA or FnBPB on the surface of L. lactis conferred fibronectin binding characteristics similar to those of S. aureus. (duke.edu)
- As well as, because the obvious molecular weights of VWF and fibronectin are virtually similar, these molecules have been tough to tell apart by SDS-polyacrylamide gel electrophoresis adopted by immunoblotting. (holliseden.com)
Expression2
- Within cohorts of soft tissue diagnoses assembled by tissue type (liposarcoma, leiomyosarcoma, undifferentiated sarcoma, and other), endosialin expression was significantly correlated with a better outcome. (hindawi.com)
- complementation of Scl1 expression in the mutant restored biofilm to wild-type level. (cdc.gov)
Vascular1
- In 1 patient, the vascular type of Ehlers-Danlos syndrome (EDS) was associated with mild hemophilia A. (medscape.com)
System1
- This method represents a functional test system for fibronectin as a contact guidance material. (ucl.ac.uk)
Surface1
- The absence of both FnBPs from the surface of S. aureus removed its ability to form a detectable bond with fibronectin. (duke.edu)
Specific1
- MRI study of the hypermobility type may document specific white matter lesions. (medscape.com)
Study1
- Rombaut et al performed a cross-sectional study of self-reported medication use, surgery, and physiotherapy in 79 patients with hypermobility-type Ehlers-Danlos syndrome (EDS) (type III), finding that 73 patients (92.4%) were taking medication, 56 (70.9%) had undergone surgery, and 41 (51.9%) were currently receiving physical therapy. (medscape.com)