Type specific anti collagen antibodies collagen type i. Medical search. Frequent questions

The development of immunoassay-based markers with monoclonal antibodies directed to the bone-specific isoform of alkaline phosphatase has improved specificity and sensitivity. (medscape.com)
The specificity of autoimmune injury to cartilaginous tissues has led investigators to test the hypothesis that a cartilage-specific autoantibody is central to the pathogenesis of relapsing polychondritis. (medscape.com)
The epitope specificity of the antibodies in relapsing polychondritis differs from those in RA, suggesting different mechanisms for formation and pathophysiologic roles. (medscape.com)
The sensitivity and specificity of the antibodies were evaluated by enzyme-linked immunosorbent assay and immunohistochemistry. (hindawi.com)
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
Tests for serum anti-GBM antibodies are most commonly used to diagnose Goodpasture syndrome because of their widespread availability and high sensitivity and specificity. (rheumaknowledgy.com)
Antibody specificity was confirmed by cross-reactivity tests. (ox.ac.uk)
The combination of em DRB1*04 /em + anti-CCP antibodies gave increase in the specificity and positive predictive value to 92% and 90 respectively. (exposed-skin-care.net)

Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. (rockland.com)
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. (rockland.com)
This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. (rockland.com)
N.B. Antibody reactivity and working conditions may vary between species. (bio-rad-antibodies.com)
they were specific for their respective antigen and displayed limited cross reactivity. (ox.ac.uk)

Our aim is to report a rare case of bullous scleroderma, studying the presence of fibronectin and collagens type I, III and XVIII in sclerodermic skin. (cdlib.org)
Indirect immunofluorescence measured by laser confocal microscopy allows quantitative determination of fibronectin and collagens type I, III and XVIII. (cdlib.org)
Conversely, both fibronectin and collagen XVIII are increased in scleroderma skin, suggesting their involvement in the pathogenesis of bullous scleroderma. (cdlib.org)
Antibodies against mouse collagen type I, III and fibronectin were raised in rabbits and purified as described elsewhere. (sdfca.org)
Sections were then incubated at 4C with the primary antibodies diluted at 1:200 (anti-collagen type I, III, and fibronectin), 1:300 (anti-MMP-2), and 1:500 (anti-MMP-9, TIMP-1, and OPN) in T-PBS. (sdfca.org)
Extracellular regions of CD206 include an N-terminal cysteine-rich (CR) domain that binds sulfated glycoproteins, a fibronectin II (FNII) domain that binds collagens, and eight carbohydrate recognition domains (CRDs) that bind sugars like mannose and fucose with high affinity. (fishersci.com)
This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. (thermofisher.com)
Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. (creative-biolabs.com)

Human ileum cryosection stained with Mouse anti Human Collagen VII antibody, clone LH7.2 ( MCA597GA ). (bio-rad-antibodies.com)
Antibodies A mouse monoclonal antibody directed against mouse MMP-2 (Clone: 6E3F8) (ab86607) and rabbit polyclonal antibodies directed against mouse MMP-9 (ab38898), TIMP-1 (ab38978), and osteopontin (ab8448) were purchased from Abcam (Cambridge, MA). (sdfca.org)
Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. (fishersci.com)
Mouse anti Human CD44 antibody, clone F10-44-2 ( MCA89 ) used for the identification of mesenchymal stem cells in mixed bone marrow cell populations by flow cytometry. (bio-rad-antibodies.com)

Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible. (covalab.com)
Affinity purification on immobilized human collagen type III with cross-absorption using immobilised human plasma proteins and human collagen type I, II, IV and IV. (yoproteins.com)
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. (rockland.com)
By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
The enhancement of tumor ECM stiffness is characterized by collagen deposition and remodeling, especially manifested by the cross-linking of collagen proteins [ 10 , 11 ]. (ijbs.com)
Increased expression of regulatory proteins such as MMP and TIMP precedes increased expression of structural proteins such as collagen and elastin, potentially mediating a controlled pattern of ECM degradation and vessel remodeling without structural failure. (sdfca.org)
OBJECTIVE: To investigate reactivities of anti-CCP to various citrullinated proteins/peptides, which represent potential autoantigens in RA, and to examine the relationship between such antibodies, and their association with genetic variants within HLA-DRB1 SE alleles. (ox.ac.uk)
Because type IV collagen and laminin are proteins found exclusively in the basement membrane, the antibodies to these two proteins selectively and specifically recognize basement membrane components. (biogenex.com)

This preferred targeting of these alpha-3 collagen chains specifically in the basal membranes of glomerular and alveolar capillaries can be explained by the higher accessible exposure of epitopes, a larger expansion of the alpha-3 collagen units, and because these alpha-3 collagen chains structurally provide higher accessibility for the targeting antibodies. (wikipedia.org)
These antibodies bind their reactive epitopes to the basement membranes and activate the complement cascade, leading to the death of tagged cells. (wikipedia.org)
Binds native 3D epitopes of human collagen type III. (yoproteins.com)
The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. (rockland.com)
The specific antigenic epitopes recognized by anti-GBM antibodies were recently shown to be present on the a3 chain of type IV collagen. (rheumaknowledgy.com)

Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. (rockland.com)
In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
1: Ritti L. Type I Collagen Purification from Rat Tail Tendons Methods Mol Biol. (moleculardepot.com)

Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. (biocheminfo.org)

It is thought to attack the alpha-3 subunit of type IV collagen, which has therefore been referred to as Goodpasture's antigen. (wikipedia.org)
The evidence for an autoimmune etiology includes pathological findings of infiltrating T cells, the presence of antigen-antibody complexes in affected cartilage, cellular and humoral responses against collagen type II and other collagen antigens, and the observation that immunosuppressive regimens most often suppress the disease. (medscape.com)
Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis. (rockland.com)
Particle-enhanced assays were based on the reaction between a soluble analyte and the corresponding antigen or antibody bound to polystyrene particles. (cdc.gov)
CRP present in the test sample forms an antigen-antibody complex with the latex particles. (cdc.gov)
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody ( MCA89 ) followed by HRP-polymer detection and DAB substrate development (low power) following antigen retrieval using citrate buffer at pH6.2. (bio-rad-antibodies.com)

The RMT2-26 monoclonal antibody specifically recognizes T cell immunoglobulin and mucin domain-2 (TIM-2) which is also known as T-cell membrane protein 2, TIMD-2, or Tim2. (bdbiosciences.com)
Description: This MR6F3 antibody recognizes mouse CD206 also known as Macrophage Mannose Receptor (MMR) or Mannose Receptor C, Type 1 (MRC1). (fishersci.com)
The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q. (origene.com)

Serum osteocalcin is considered a specific marker of osteoblast function, as its levels correlate with the bone formation rate. (medscape.com)
Mice with collagen-induced arthritis (CIA) were treated with polyclonal rabbit anti-murine IL-17 (anti-IL-17) antibody-positive serum or normal rabbit serum after the first signs of arthritis. (nih.gov)
In addition, during a later stage of CIA mice were selected and treated with anti-IL-17 antibody or control serum. (nih.gov)
Imiquimod (Aldara) The sections were treated with 0.3% hydrogen peroxide in methanol for 30 min at room temperature to block endogenous peroxidase activity and incubated with 5% normal goat serum in PBS (pH 7.4) containing 0.05% Imiquimod (Aldara) Triton X-100 (T-PBS) for 1 h at room temperature to block non-specific protein binding sites. (sdfca.org)

GPS is caused by abnormal plasma cell production of anti-GBM antibodies. (wikipedia.org)
In contrast to some other pulmonary renal syndromes, such as SLE, production of anti-GBM antibodies is usually self-limited. (rheumaknowledgy.com)

Immunofluorescent staining of biopsy specimens from the lungs and kidneys of patients with Goodpasture syndrome reveals a characteristic linear pattern of anti-GBM antibody deposition. (rheumaknowledgy.com)
Anti-human CCN2 antibody inhibits collagen deposition in lungs of bleomycin-treated B-hCCN2 mice. (biocytogen.com.cn)
Functional assays (Ref.1): Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. (origene.com)

Multiple antibody reactivities to citrullinated antigens in sera from patients with rheumatoid arthritis: association with HLA-DRB1 alleles. (ox.ac.uk)
CONCLUSIONS: Antibodies directed against several citrullinated antigens are present in CCP-positive RA, with many patients displaying multireactivity. (ox.ac.uk)

Conclusion Our findings suggest that anti-CCP2 is usually superior to Rabbit polyclonal to AKIRIN2 RF for the detection of RA and provided predictive information on joint destruction and disease activity. (exposed-skin-care.net)

Applications Reported: This MR6F3 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis. (fishersci.com)

Chicken type II collagen was used to induce CIA in mice, which were then treated with an anti-human DR5 antibody, TRA-8. (nih.gov)
En conclusión, encontramos que el TAA induce la lesión renal en asociación con el aumento del eje ROS/TNF-α, independientemente de la infiltración de leucocitos, que está protegida por metformina. (bvsalud.org)
Initial studies demonstrated that rabbit antimouse type VII collagen antibodies, as well as rabbit antibodies raised against human recombinant protein type VII collagen, were able to induce blisters in mice. (medscape.com)
[ 7 ] The failure of rabbit antimouse type VII collagen antibodies to induce blisters in C5-deficient mice supports a role for complement activation in the disease pathogenesis. (medscape.com)
Immunization of type VII collagen in athymic nude SJL mice did not induce an autoimmune response, whereas the repletion of T cells from type VII collagen-immunized wild-type mice to the thymic mice showed autoantibody production and resulted in a blistering disease phenotype, supporting the role of T cells in the induction of epidermolysis bullosa acquisita. (medscape.com)

Autoantibodies to minor cartilage-specific collagens (ie, types IX and XI) have been described. (medscape.com)
BACKGROUND: Autoantibodies to cyclic citrullinated peptides (anti-CCP) are present in most patients with rheumatoid arthritis (RA), and associate with HLA-DRB1 shared epitope (SE) alleles. (ox.ac.uk)
They could also be triggered by autoantibodies, like anti-neutrophil cytoplasmic antibodies, or ANCA. (osmosis.org)
In canine epidermolysis bullosa acquisita, the immunoglobulin G (IgG) autoantibodies also target the type VII collagen noncollagenous (NC1) domain, which shares greater than 80% homology in amino acid sequence with the human NC1 domain. (medscape.com)
A subset of patients have variations in which either IgG autoantibodies bind the central triple-helical collagenous (NC2) domain of type VII collagen, or immunoglobulin A (IgA), rather than IgG, targets type VII collagen. (medscape.com)
[ 3 ] IgG autoantibodies specific for anchoring fibrils (type VII collagen) of the skin basement membrane have a heterogeneous subclass and light-chain composition, and their complement-activating capacities do not correlate with the inflammatory phenotype. (medscape.com)
[ 5 , 6 ] More recently, affinity-purified antitype VII collagen autoantibodies from epidermolysis bullosa acquisita patients have induced blisters in an adult hairless mouse strain (SKH1), further supporting a pathogenic role of antitype collagen VII autoantibodies. (medscape.com)
Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)

Rockland produces highly active antibodies and conjugates to collagens. (rockland.com)

The major target of these abnormal antibodies is the non-collagen domain of the alpha-3 chain of type 4 collagen, which is mostly found in the basal membranes of glomerular and alveolar capillaries, explaining the obscurely specific symptoms of this condition. (wikipedia.org)

The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. (bdbiosciences.com)
Find COL4A1 Antibodies with a specific conjugate such as Biotin, FITC, HRP. (antibodies-online.com)

To determine the therapeutic efficacy and immunomodulatory effect of an anti-human death receptor 5 (DR5) antibody, TRA-8, in eliminating macrophage subsets in a mouse model of type II collagen-induced arthritis (CIA). (nih.gov)
Our data strongly indicate that IL-17 neutralization could provide an additional therapeutic strategy for RA, particularly in situations in which elevated IL-17 may attenuate the response to anti-tumor necrosis factor/anti-IL-1 therapy. (nih.gov)
It is also a key mediator of immune inflammatory hypernociception normally associated with a Th1 type of response, revealing a hitherto unrecognized function of IL-33 in a key immune pharmacological pathway that may be amenable to therapeutic intervention [ 13 ]. (hindawi.com)
Finally, we investigated the therapeutic effect of a monoclonal antibody specifically blocking the binding of VEGF to neuropilin (NRP)-1. (ox.ac.uk)
Importantly, therapeutic administration of anti-NRP1B antibody significantly reduced disease severity and progression in CIA mice. (ox.ac.uk)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

They are more likely to be found in association with antibodies to type II collagen in patients with relapsing polychondritis. (medscape.com)
Goodpasture syndrome is an autoimmune disorder characterized by pulmonary hemorrhage and rapidly progressive glomerulonephritis in association with antibodies to alveolar and glomerular basement membrane (GBM). (rheumaknowledgy.com)

HA affects certain antibodies and cell receptors involved in cell adhesion, which reduces the formation of scar tissue after injury or surgery. (stoneclinic.com)
Excess collagen formation is another mechanism by which scar tissue forms after an injury. (stoneclinic.com)
Despite these technical advances, the important unsolved challenges that these methods face include complexity, time, consistency of tissue size before and after clearing, and ability to immunolabel various antibodies in cleared tissue. (en-journal.org)
Adipose tissue-derived stem cells (ADSCs) have been characterized as having the ability to self-renew and differentiate into different connective tissue cells, including osteoblasts, adipocytes, chondrocytes and myocytes, under specific inductive stimuli ( 4 ). (spandidos-publications.com)
Evaluation of the efficacy of proposed agents in an in vitro model is convenient and allows assessment of the mechanical properties of the bone-tendon interface as well as the effect on fibroblast proliferation and collagen content of the tissue. (researchgate.net)
4: Michopoulou A, Koliakou E, Terzopoulou Z, Rousselle P, Palamidi A, Anestakis D, Konstantinidou P, Roig-Rosello E, Demiri E, Bikiaris D. Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth J Biomed Mater Res A. 2022 Apr;110(4):797-811. (moleculardepot.com)
9: Mian E, Mian M, Begh F. Lyophilized type-I collagen and chronic leg ulcers Int J Tissue React. (moleculardepot.com)
Kidney tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN2 mice (H/H), and analyzed by western blot with anti-CCN2 antibody. (biocytogen.com.cn)
Spleen and kidney tissue of wild-type mice (Ref.1). (origene.com)
We report finding anti-AMDV antibodies and AMDV genome in tissue from 2 mink farmers with relevant virus exposure and clinical disease similar to that in mink with AD. (cdc.gov)

Examples of such an insult include: exposure to organic solvents (e.g. chloroform) or hydrocarbons, exposure to tobacco smoke, infection (such as influenza A), cocaine inhalation, metal dust inhalation, bacteremia, sepsis, high-oxygen environments, and antilymphocyte therapies (especially with monoclonal antibodies). (wikipedia.org)

Systemic IL-6 levels were measured by enzyme-linked immunosorbent assay, and local synovial IL-1 and receptor activator of NF-kappaB ligand (RANKL) expression was analyzed using specific immunohistochemistry. (nih.gov)
Endostatin, an anti-angiogenic C-terminal fragment of collagen XVIII, has been recently reported to play a role in scleroderma pathogenesis, but collagen XVIII immunohistochemistry in scleroderma skin has still not been performed. (cdlib.org)
This antibody can be used for applications: Immunohistochemistry, Immunoprecipitation, Western Blotting. (creative-biolabs.com)

The collagen domain mediates trimer formation. (rndsystems.com)

Furthermore, levels of antibodies to matrilin 1, an extracellular matrix protein predominantly expressed in tracheal cartilage, were significantly higher in patients with relapsing polychondritis, especially in those with respiratory symptoms, than in patients with Wegener granulomatosis , systemic lupus erythematosus , or RA and in healthy controls. (medscape.com)
The correct type of extracellular matrix was required, since only a basement membrane, not plastic or a collagen I matrix, lowered the rate of apoptosis. (brighton.ac.uk)
Anti-3M-1 mAbs (alpha 3M-1-Ab) were used to positively select for 3M-1-secreting tubular epithelium and, after stabilization in culture, this new cell line (MCT) was examined for the production of several moieties important to either immune interactions or to the development of extracellular matrix. (rupress.org)

The antibody detects endogenous Collagen II protein. (biocheminfo.org)
The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)
Anti-CCP and anti-citrullinated protein antibodies were associated with HLA-DRB1*04 rather than with HLA-DRB1*01 alleles. (ox.ac.uk)

A specific antibody and epitope binding that shows the highest affinity and is pathogenic occurs between GPA antibodies and the anti-GBM epitope region, designated EA, which is residues 17-31 of the alpha 3 subunit of non-collagenous domain of type IV collagen. (wikipedia.org)
Find COL4A1 Antibodies with a specific epitope. (antibodies-online.com)

As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1). (origene.com)
Dashed histograms indicate staining with isotype-matched control antibody and solid histograms denote the specific expression of each indicated marker. (bio-rad-antibodies.com)

Overall process is designed to obtain a high purity type-specific antibody to native 3D collagen. (yoproteins.com)

Laminin and type IV collagen are the two principal components of the basement membrane. (biogenex.com)
MCT culture supernatants contain greater amounts of laminin, and types IV and V procollagens compared to types I and III procollagens, and growing MCT cells on type I collagen matrix causes them to preferentially secrete even more type IV and V procollagen. (rupress.org)

[ 8 ] A report of relapsing polychondritis in the newborn of a mother with relapsing polychondritis suggests that antibodies crossing the placenta are necessary and sufficient to elicit the entire clinical syndrome. (medscape.com)
Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. (rockland.com)

Various biochemical markers are now available that allow a specific and sensitive assessment of the rate of bone formation and bone resorption of the skeleton. (medscape.com)
Gene expression profiling and functional analysis of angiogenic markers in murine collagen-induced arthritis. (ox.ac.uk)

An experimental toolbox for characterization of mammalian collagen type I in biological specimens Nat Protoc. (moleculardepot.com)

Various studies find circulating antibodies to cartilage-specific collagen types II, IX, and XI to be present in 30%-70% of patients with relapsing polychondritis. (medscape.com)
Most patients with relapsing polychondritis had high titers of antifetal cartilage antibodies during the early acute phase. (medscape.com)
The antifetal cartilage antibodies were found in 6 of 9 patients and only 4 (1.5%) of 260 patients with RA, exclusively in long-standing disease. (medscape.com)
Histologic analysis confirmed the suppression of joint inflammation and showed prevention of cartilage and bone destruction after anti-IL-17 antibody therapy. (nih.gov)
HA suppresses the gene expression for the specific types of collagen involved in the formation of scars, but not the collagens involved in the formation of normal articular cartilage. (stoneclinic.com)

The pathogenesis of Goodpasture's syndrome is related to anti-GBM antibodies. (rheumaknowledgy.com)

Goodpasture syndrome (GPS), also known as anti-glomerular basement membrane disease, is a rare autoimmune disease in which antibodies attack the basement membrane in lungs and kidneys, leading to bleeding from the lungs, glomerulonephritis, and kidney failure. (wikipedia.org)
The anti-glomerular basement membrane (GBM) antibodies primarily attack the kidneys and lungs, although, generalized symptoms like malaise, weight loss, fatigue, fever, and chills are also common, as are joint aches and pains. (wikipedia.org)
In addition to genetic susceptibility, an initial environmental insult to the pulmonary vasculature is needed to allow the anti-glomerular basement membrane (anti-GBM) antibodies to reach the alveolar capillaries. (wikipedia.org)
Antiglomerular basement membrane antibody disease. (rheumaknowledgy.com)
Type IV collagen is the major component of basement membranes and provides structural support. (rheumaknowledgy.com)
95% of patients with Goodpasture syndrome (see Antiglomerular Basement Membrane (GBM) Antibody ). (rheumaknowledgy.com)
The diagnosis of Goodpasture syndrome may be secured by demonstrating linear antibody staining of the basement membrane of renal or pulmonary biopsy specimens. (rheumaknowledgy.com)
Long-term outcome of anti-glomerular basement membrane antibody disease treated with plasma exchange and immunosuppression. (rheumaknowledgy.com)
A function-blocking anti-b1 integrin antibody doubled the rate of apoptosis in single cells cultured with basement membrane, indicating that integrin-mediated signals contributed to survival. (brighton.ac.uk)
We propose that basement membrane provides a survival stimulus for epithelial cells in vivo, and that loss of interaction with this type of matrix acts as a control point for cell deletions that occur at specific times during development, such as in mammary gland involution. (brighton.ac.uk)
This gene encodes the major type IV alpha collagen chain of basement membranes. (antibodies-online.com)
Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
[ 4 ] These antibodies compromise the strength of the basement membrane zone, leading to skin fragility and trauma-induced blister formation. (medscape.com)

In adult mink, AD is a persistent, slowly progressive AMDV infection in which a dysregulated immune system and a postinfectious antibody response cause an immune complex-mediated vasculitis ( 2 ). (cdc.gov)

Among the candidate autoantigens examined, IgG antibodies to citrullinated fibrinogen were found in 66% of patients' sera and in 41% for both citrullinated alpha-enolase peptide-1 and citrullinated C1(III). (ox.ac.uk)
The 35‑40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. (rndsystems.com)

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. (thermofisher.com)

Strain specific analysis of CCN2 gene expression in wild-type mice and B-hCCN2 mice by RT-PCR. (biocytogen.com.cn)
Human CCN2 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice. (biocytogen.com.cn)
CCN2 was detectable in wild type mice and homozygous B-hCCN2 mice, as the antibody is crossly reactive with CCN2 in human and mice. (biocytogen.com.cn)

The following product was used in this experiment: CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Biotin, eBioscience™ from Thermo Fisher Scientific, catalog # 13-0519-82, RRID AB_529536. (thermofisher.com)

On top of the anti-GBM antibodies implicated in the disease, about one in three of those affected also has cytoplasmic antineutrophilic antibodies in their bloodstream, which often predates the anti-GBM antibodies by about a few months or even years. (wikipedia.org)

Freeze-dried, cross-linked bovine type I collagen: analysis of properties J Periodontol. (moleculardepot.com)

it has been proposed that antibodies are formed as a primary event in relapsing polychondritis. (medscape.com)
70% of primary cases are associated with anti-PLA2R antibodies. (shiken.ai)

A human/mouse-chimeric DR5-transgenic mouse, under the regulation of a mouse 3-kb promoter and a loxP-flanked STOP cassette, was generated and crossed with an ubiquitous Cre (Ubc.Cre) mouse and a lysozyme M-Cre (LysM.Cre)-transgenic mouse to achieve inducible or macrophage-specific expression. (nih.gov)
The anti-human DR5 antibody TRA-8 was efficacious in reducing the severity of arthritis via targeted depletion of macrophages and immunomodulation. (nih.gov)
Detection of human collagen type III by ELISA (x3000), Histochemistry (frozen sections) (x20). (yoproteins.com)
Human Type I Collagen Lyophilized is a high quality Human Type I Collagen Lyophilized. (moleculardepot.com)
Immunoquantification of type I, III, IV and V collagen in small samples of human lung parenchyma Int J Biochem Cell Biol. (moleculardepot.com)
7: Liu X, Wang J, Dong F, Song P, Tian S, Li H, Hou Y. Cytocompatibility and biologic characteristics of synthetic scaffold materials of rabbit acellular vascular matrix combining with human-like collagen I J Biomater Appl. (moleculardepot.com)
10: Hou Z, Li X, Li M, Yang J, Yang X, Zhao J. [Preparation and characterization of recombinant human-source collagen] Sheng Wu Gong Cheng Xue Bao. (moleculardepot.com)
Staining of M21 cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.5 µg of Anti-Human CD51/CD61 (Integrin alpha/beta 3) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). (thermofisher.com)
B-hCCN2 mice were assigned to one saline non-bleomycin control group (n=4) and two bleomycin-treated fibrosis groups (n=9) which were treated with pamrevlumab (in house, anti-human CCN2) or vehicle. (biocytogen.com.cn)
Staining of human peripheral blood granulocytes with Mouse anti Human CD44:Alexa Fluor ® 647 ( MCA89A647 ). (bio-rad-antibodies.com)
Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:FITC ( MCA89F ). (bio-rad-antibodies.com)
Find COL4A1 Antibodies for a variety of species such as anti-Human COL4A1, anti-Mouse COL4A1, anti-Rat COL4A1. (antibodies-online.com)

Various murine models have contributed to the understanding of the pathogenic role of antitype VII collagen antibodies and pathophysiology of epidermolysis bullosa acquisita. (medscape.com)

HA suppresses the genetic expression of several inflammatory molecules, acting as an anti-inflammatory and reducing joint swelling. (stoneclinic.com)
For example, acetaminophen and non-steroidal anti-inflammatory drugs are commonly prescribed for pain relief in patients with OA. (spandidos-publications.com)
Since vasculitides are systemic inflammatory disorders, non-specific symptoms like fever, arthritis , myalgia, weight loss, and night sweats might be present. (osmosis.org)
If you develop arthritis symptoms, you might benefit from nonsteroidal anti-inflammatory drugs (NSAIDs) or other arthritis medications. (mayoclinic.org)
When platelet function is reduced (eg, as a result of uremia, or use of nonsteroidal anti-inflammatory drug [NSAID] or aspirin), the risk of bleeding increases. (msdmanuals.com)

The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. (origene.com)

Significant expression of IL-33 was seen in mouse liver and lung tissues determined with the anti-IL-33. (hindawi.com)
Collagen Type I extracted and purified from mouse skin. (affipcr.com)
En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
Applications Tested: This MR6F3 antibody has been tested by intracellular staining and flow cytometric analysis of mouse resident peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. (fishersci.com)
To provide a more profound understanding of arthritis-associated angiogenesis, we evaluated the expression of angiogenesis-modulating genes at onset, peak and declining phases of collagen-induced arthritis (CIA), a well-established mouse model for RA. (ox.ac.uk)
A dilute solution of test sample was mixed with latex particles coated with mouse monoclonal anti-CRP antibodies. (cdc.gov)
This product is a HRP-conjugated anti-mouse CD36 monoclonal antibody gernerated from the mouse. (creative-biolabs.com)

Please refer to the Certificate of Analysis for the lot-specific concentration. (affipcr.com)
An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

TIM-2 is an ~55 kDa type I transmembrane glycoprotein that is encoded by Timd2 (T cell immunoglobulin and mucin domain containing 2) which belongs to the immunoglobulin gene superfamily. (bdbiosciences.com)

In addition, if there is substantial suspicion of the disease, seralogic testing for ELISA assay is usually done by looking for alpha3 NC1 domain area of collagen IV in order to avoid false positives. (wikipedia.org)
Find COL4A1 Antibodies validated for a specific application such as WB, ELISA, IHC, IHC (p). (antibodies-online.com)

In GPS, anti-GBM antibodies are produced and circulated throughout the bloodstream, damaging the membranes lining the lungs and kidneys as well as targeting their capillaries. (wikipedia.org)

Samples containing BAP were reacted with a biotin-labeled, BAP-specific monoclonal antibody. (cdc.gov)

Thick and hyaline collagen fibers are found, as well as lymphatic ectasia and pilosebaceous atrophy that extends to the hypodermic region (Fig. 2). (cdlib.org)

Anatomy43

Organisms11

Diseases13

Chemicals and Drugs117

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes41

Disciplines and Occupations2

Technology, Industry, Agriculture3

Information Science3

Specificity8

Reactivity5

Fibronectin8

Clone4

Proteins8

Epitopes5

Purification3

Immunogen1

Antigen6

Recognizes3

Serum4

Production of anti-GBM antibodies2

Deposition3

Antigens2

Rabbit Polyclonal1

Followed by flow cytometric analysis1

Induce5

Autoantibodies8

Conjugates1

Chain of type 4 collag1

Conjugate2

Therapeutic5

Ascites by affinity-chromatography1

Association with antibodies2

Tissue10

Monoclonal antibodies1

Immunohistochemistry3

Domain mediates1

Extracellular3

Protein3

Epitope2

Isotype matched2

Purity1

Laminin2

Placenta2

Markers2

Mammalian1

Cartilage5

Pathogenesis1

Basement13

Immune complex-mediated1

Fibrinogen2

Reacts1

Wild type mice3

EBioscience1

Cytoplasmic1

Bovine1

Primary2

Human12

Pathophysiology1

Inflammatory5

Immunization1

Mouse7

Concentration2

Immunoglobulin1

ELISA2

Lungs1

Biotin1

Hyaline1