Type i cyclic amp dependent protein kinase. Medical search. Frequent questions

Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition. (rush.edu)
CONTEXT: Carney complex (CNC) is an autosomal dominant condition caused, in most cases, by an inactivating mutation of the PRKAR1A gene, which encodes for the type 1 alpha regulatory subunit of protein kinase A. CNC is characterized by the occurrence of endocrine overactivity, myxomas and typical skin manifestations. (kl.ac.at)
n -Tetradecanoyl is the NH 2 -terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle. (chemistryviews.org)
Interestingly, recombinant protein of wild-type PDE10A2, but not PDE10A2 mutant with an Ala replacement at Thr 16 , was distributed to cytosolic fractions by co-transfection with a plasmid encoding the catalytic subunit of PKA. (johnshopkins.edu)
Harris BA: Complete cDNA sequence of a human stimulatory GTP-binding protein alpha subunit. (smpdb.ca)
Somatic mutations of the catalytic subunit of cyclic AMP-dependent protein kinase (PRKACA) gene in Japanese patients with several adrenal adenomas secreting cortisol [Rapid Communication]. (cdc.gov)
Novel somatic mutations in the catalytic subunit of the protein kinase A as a cause of adrenal Cushing's syndrome: a European multicentric study. (cdc.gov)

A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. (rush.edu)
T739 phosphorylation is normally governed by CaMKII and possibly kinase assay where we incubated GSTCNL-1 (outrageous type or T739A), GSTCNL-2, GSTCNL-3 and GSTCNL-4 c-tail fusion protein with CaMKII and ATP. (findadig.com)
We solved the protein by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Bismuth Subcitrate Potassium and immunoblotting uncovered which the phosphorylation stateCspecific antibody particularly recognized just the NL-1 c-tail that's phosphorylated at T739 (Fig. 2a). (findadig.com)
Translocation and structure switch of STING provides the prerequisite for TANK-binding kinase 1 (TBK1) recruitment and auto-phosphorylation. (frontiersin.org)
Using FRET-based sensors to monitor cAMP and PKA-dependent phosphorylation in the cytosol and outer mitochondrial membrane (OMM) of primary rat cardiomyocytes, we demonstrate that comparable cAMP increases in these two compartments evoke higher levels of PKA-dependent phosphorylation in the OMM. (ox.ac.uk)
We present a model that accounts for these unexpected results in which the degree of PKA-dependent phosphorylation is dictated by both the subcellular distribution of the phosphatases and the different accessibility of membrane-bound and soluble phosphorylated substrates to the cytosolic enzymes. (ox.ac.uk)
In isolated nerve terminals, rapid changes in synapsin I phosphorylation were observed after Ca2+ entry, namely, a Ca2+-dependent phosphorylation of P-sites 1, 2, and 3 and a Ca2+-dependent dephosphorylation of P-sites 4, 5, and 6. (ucl.ac.uk)

PDE10A2 was phosphorylated by cAMP-dependent protein kinase (PKA) at Thr 16 . (johnshopkins.edu)

Thus, our discovery of the role of functional interaction between intracellular signaling pathways mediated by calcium ions (Ca 2+) and cyclic adenosine monophosphate (cAMP) (Ca 2+ /cAMP signaling interaction) in these cellular responses, opened a great avenue for the development of new antitumor therapeutic strategies. (researchgate.net)
Cyclic adenosine monophosphate ( cAMP , cyclic AMP , or 3',5'-cyclic adenosine monophosphate ) is a second messenger , or cellular signal occurring within cells, that is important in many biological processes. (wikipedia.org)
cAMP is a derivative of adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying the cAMP-dependent pathway . (wikipedia.org)
Earl Sutherland of Vanderbilt University won a Nobel Prize in Physiology or Medicine in 1971 "for his discoveries concerning the mechanisms of the action of hormones", especially epinephrine, via second messengers (such as cyclic adenosine monophosphate, cyclic AMP). (wikipedia.org)
We show here that cyclic adenosine diphosphate-ribose (cADPR), by activating RyRs, mediates, in part, hyperpolarization and vasodilation by beta-adrenoceptors. (ox.ac.uk)
Beta-adrenergic stimulation activates adenylate cyclase, which increases cyclic adenosine monophosphate (AMP) levels and causes increased calcium influx into myocardial cells. (medscape.com)

Type 4 phosphodiesterases (PDE4) are key cAMP-hydrolyzing enzymes, and PDE4 inhibitors are considered as immunosuppressors to various inflammatory responses. (ox.ac.uk)
Considerably higher resistance against cyclic nucleotide phosphodiesterases compared to dibutyryl- or 8-Br-cAMP (Cat. (biolog.de)
Evidence supporting the heterogeneity in cAMP and PKA signaling is rapidly accumulating and has been largely attributed to the localization or activity of adenylate cyclases, phosphodiesterases, and A-kinase-anchoring proteins in different cellular subcompartments. (ox.ac.uk)
A kinase anchoring proteins (AKAPs) compromise the largest group of multifunctional scaffolding proteins, which anchor not only Type II cAMP- dependent protein kinase (PKA), but also a variety of protein kinases, phosphatases, phosphodiesterases and targets of second messenger regulated signaling. (grantome.com)

Impact of beta and gamma variants on ligand-binding properties of gamma-aminobutyric acid type A receptors. (aspetjournals.org)
5-Hydroxytryptamine type 2A receptors regulate cyclic AMP accumulation in a neuronal cell line by protein kinase C-dependent and calcium/calmodulin-dependent mechanisms. (aspetjournals.org)
Transfected muscarinic acetylcholine receptors selectively couple to Gi-type G proteins and Gq/11. (aspetjournals.org)
[1] Adenylate cyclase is activated by a range of signaling molecules through the activation of adenylate cyclase stimulatory G ( G s )-protein-coupled receptors. (wikipedia.org)
Adenylate cyclase is inhibited by agonists of adenylate cyclase inhibitory G ( G i )-protein-coupled receptors. (wikipedia.org)
Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning. (tamu.edu)
Here we report that extinction is triggered by two hippocampal signaling pathways underlying retrieval (the cAMP-dependent protein kinase and the mitogen-activated protein kinase pathways) and two other mechanisms that become activated at the same time and are not necessary for retrieval (N-methyl-D-aspartate glutamatergic receptors and the calcium/calmodulin-dependent protein kinase II signaling pathway). (unboundmedicine.com)
We conclude, therefore, that relaxation of arterial smooth muscle by adenylyl cyclase-coupled receptors results, in part, from a cAMP-dependent and protein kinase A-dependent increase in cADPR synthesis, and subsequent activation of sarcoplasmic reticulum Ca(2+) release via RyRs, which leads to activation of BK(Ca) channels and membrane hyperpolarization. (ox.ac.uk)

We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)
Glucose metabolism activates this pathway by means of increasing [Ca 2+ ] i via L-type voltage-dependent calcium channels (VDCCs) by affecting the electrical activity of the cell. (diabetesjournals.org)
Vasodilation by the calcium-mobilizing messenger cyclic ADP-ribose. (ox.ac.uk)

The Tax protein has pleiotropic effects on host-cell gene expression and activates several pathways such as the cyclic AMP responsive binding protein (CREB), the nuclear factor kappa-B (NF-κB), the cyclin-dependant kinases (CDKs), and the Akt pathways. (nih.gov)
The Tax protein has pleiotropic effects on host-cell gene expression and activates several pathways such as the cyclic AMP responsive binding protein (CREB), the nuclear factor kappa-B, the cyclin-dependant kinases (CDKs), and the Akt pathways. (nih.gov)
These data suggest that the synergistic action of glucose and GLP-1 to promote insulin gene transcription is mediated through NFAT via PKA- and calcineurin-dependent pathways in pancreatic β-cells. (diabetesjournals.org)
We examined the role of cGMP-dependent signaling pathways and intracellular Ca(2+)stores in mediating the positive chronotropic effect of NO donors. (ox.ac.uk)
These results suggest that independent pp60v-src and PKC pathways converge at Ras and that PKA acts to block MAPK activation by both Ras-dependent and -independent signals. (ox.ac.uk)

In 1998 a family of cAMP-sensitive proteins with guanine nucleotide exchange factor (GEF) activity was discovered. (wikipedia.org)

Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase. (musc.edu)
b) Immunoblot evaluation of NL-1 (outrageous type or T739A) transfected in COS cells and treated using a CaMKII inhibitor, KN93, or cotransfected with constitutively energetic CaMKII (T286D). (findadig.com)
VIP's lack of effect on IL-10 and its slight effect on TNF-alpha results from cAMP being rapidly degraded as the phosphodiesterase IV inhibitor, rolipram, rescues cAMP-dependent activation of cAMP response element binding protein. (ox.ac.uk)
We have purified and characterized several GDP/GTP exchange proteins (GDP dissociation stimulator (GDS) and GDP dissociation inhibitor (GDI)) and GTPase activating Proteins (GAP) for small G proteins. (nii.ac.jp)
Significantly, the actions of both GLP-1 and forskolin were abolished by the selective Ca 2+ /calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, FK506, as well as by the chelation of intracellular Ca 2+ by BAPTA (bis(2-aminophenoxy)ethane- N,N,N ′ ,N ′-tetraacetate). (diabetesjournals.org)
Furthermore, inhibition of endogenous PKA by the heat-stable PKA inhibitor is sufficient to stimulate MAPK activity in these extracts in the absence of protein synthesis and without dependence on a functional Ras protein. (ox.ac.uk)

Phosphatases control PKA-dependent functional microdomains at the outer mitochondrial membrane. (ox.ac.uk)
Furthermore, we show that the activity of soluble phosphatases attenuates PKA-driven activation of the cAMP response element-binding protein while concurrently enhancing PKA-dependent mitochondrial elongation. (ox.ac.uk)
PKN, casein kinase 1, phosphodiesterase 4D3, protein phosphatases 1 and 2a and calmodulin as well as a number of putative downstream effectors. (grantome.com)

Herpes simplex virus protein kinase US3 activates and functionally overlaps protein kinase A to block apoptosis. (uchicago.edu)
As the second messenger, cGAMP activates STING and induces the following cascade to produce type I interferon (IFN-I) to protect against infections. (frontiersin.org)
The FSH receptor activates G(s) proteins which leads to the activation of adenylyl cyclase and produces the secondary messenger cAMP. (smpdb.ca)
cAMP then activates protein kinase A (PKA) which phosphorylates downstream effectors that lead to a specific cellular response. (smpdb.ca)

P-sites 2 and 3 phosphorylated by Ca2+ calmodulin-dependent protein kinase II) were excellent substrates for protein phosphatase 2A, whereas P-sites 4, 5, and 6 (phosphorylated by mitogen-activated protein kinase) were efficiently dephosphorylated only by Ca2+-calmodulin-dependent protein phosphatase 2B-calcineurin. (ucl.ac.uk)
Regulation of mitogen-activated protein kinase activation by protein kinases A and C in a cell-free system. (ox.ac.uk)
Previously pp60v-src, cyclin A, p39mos, and maturation-promoting factor (composed of Cdc2 and cyclin B) have been shown to activate mitogen-activated protein kinase (MAPK) and MAPK kinase (MEK) in cell-free extracts of Xenopus oocytes. (ox.ac.uk)

Nitric oxide (NO) donors increase heart rate (HR) through a guanylyl cyclase-dependent stimulation of the pacemaker current I(f), without affecting basal I(Ca-L). The activity of I(f)is known to be enhanced by cyclic nucleotides and by an increase in cytosolic Ca(2+). (ox.ac.uk)

Elevation of glutathione levels by phase II enzyme inducers: lack of inhibition of human immunodeficiency virus type 1 replication in chronically infected monocytoid cells. (aspetjournals.org)
Effects of tyrphostins on the activated c-src protein in NIH/3T3 cells. (aspetjournals.org)
Here we demonstrate that elevation of cyclic AMP (cAMP) levels in human umbilical vein endothelial cells (HUVECs) specifically attenuates ERK1,2 activation in response to either leptin or a soluble interleukin IL-6 receptor-α/IL-6 (sIL-6R-α/IL-6) trans-signalling complex but not protein kinase C activator phorbol 12-myristate 13-acetate. (gla.ac.uk)
Bismuth Subcitrate Potassium (c) Cotransfection of NL-1 (outrageous type or T739A) with CaMKII (T286D) in HEK293T cells. (findadig.com)
Tax confers immortalization, anchorage-dependent cell growth, and tumorigenicity to rodent cells. (nih.gov)
Tax-expressing cells also exhibit nuclear morphological aberrancy, frequent multinucleation, aneuploidy, and loss of function of the tumor suppressor protein p53. (nih.gov)
Correction: Stoichiometry of Nck-dependent actin polymerization in living cells. (tamu.edu)
IL-25 augments type 2 immune responses by enhancing the expansion and functions of TSLP-DC-activated Th2 memory cells. (tamu.edu)
The cyclic GMP-AMP synthase (cGAS)-Stimulator of Interferon Genes (STING) pathway is a critical defender to sense various pathogens and trigger innate immunity of mammalian cells. (frontiersin.org)
The models of most immune evasion during innate immune responses are conserved, including altering the post-transcriptional modifications (PTMs) of vital proteins to inactivate or degrade these components, eluding DNA sensing from PRRs, decreasing cyclic GMP-AMP (cGAMP) cellular abundance, and modifying metabolism approach in host cells ( Eaglesham and Kranzusch, 2020 ). (frontiersin.org)
Research over the last decade has revealed that lithium's effects as mood stabilization and regeneration of new brain cells (neurogenesis), are mainly due to the inhibition of a key enzyme called GSK-3β, glycogen synthase kinase-3β. (jeffreydachmd.com)
Subcellular sequestration of second messenger-dependent mechanisms provides for specificity of signaling to discrete organelle systems or, in the case of polarized epithelial cells, plasma membrane domains. (grantome.com)
Importantly, while previous investigations have focused attention on AKAP350 at the centrosome and the Golgi apparatus, our recent studies have led to the recognition that in most cells a large cytosolic pool of AKAP350 associates with CCAR1 and caprin and regulates mRNA trafficking and participates in microtubule-dependent stress granule formation. (grantome.com)
These complexes appear to coordinate the movement intracellular proteins and messenger RNAs responsible for far ranging processes within cells. (grantome.com)
These processes can have diverse influences on the proper functioning of cells including the production and targeting of proteins to membrane surfaces and the response of cells to various induced stresses in normal and pathological conditions. (grantome.com)

Cyclic AMP-Dependent Protein Kinase Type II" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)

Woolson, H. D. , Thomson, V. S. , Rutherford, C. , Yarwood, S. J. and Palmer, T. M. (2009) Selective inhibition of cytokine-activated extracellular signal-regulated kinase by cyclic amp via Epac1-dependent induction of suppressor of cytokine signalling-3. (gla.ac.uk)
The inhibitory effect of cAMP could not be reversed by inhibition of cAMP-dependent protein kinase (PKA) but was blocked by depletion of the alternative intracellular cAMP sensor exchange protein activated by cAMP 1 (Epac1), which is also required to observe SOCS-3 accumulation in response to cAMP. (gla.ac.uk)
Inhibition of calcineurin activity by cyclosporin A resulted in a complete block of Ca2+-dependent dephosphorylation of P-sites 4, 5, and 6 and correlated with a prominent increase in ionomycin-evoked glutamate release. (ucl.ac.uk)

cAMP is associated with kinases function in several biochemical processes, including the regulation of glycogen , sugar , and lipid metabolism . (wikipedia.org)
A class of protein-serine-threonine kinases that was originally found as one of the three types of kinases that phosphorylate GLYCOGEN SYNTHASE. (rush.edu)
This graph shows the total number of publications written about "Glycogen Synthase Kinases" by people in this website by year, and whether "Glycogen Synthase Kinases" was a major or minor topic of these publications. (rush.edu)
Below are the most recent publications written about "Glycogen Synthase Kinases" by people in Profiles. (rush.edu)

Cyclic AMP binds to specific locations on the regulatory units of the protein kinase, and causes dissociation between the regulatory and catalytic subunits, thus enabling those catalytic units to phosphorylate substrate proteins. (wikipedia.org)
Protein kinase A can also phosphorylate specific proteins that bind to promoter regions of DNA, causing increases in transcription. (wikipedia.org)
Furthermore, to detect whether PKC or PKA have the ability to phosphorylate NL-1 T739, we examined GSTCNL-1 after kinase reactions using liquid chromatography combined to tandem mass spectrometry (LC/MS/MS) and discovered that just CaMKII phosphorylates T739 (Fig. 1eCg). (findadig.com)

In addition, it reveals a novel Epac-dependent mechanism by which cAMP can specifically inhibit ERK in response to cytokine receptor activation. (gla.ac.uk)
Cytokine production was assessed for cell cultures and, in addition, the ability of VIPs to activate cAMP response element binding protein. (ox.ac.uk)
Interestingly, macrophages stimulated with phorbol 12-myristate 13-acetate/ionomycin displayed an augmented IL-10 response upon addition of dibutyryl cAMP, with corresponding downregulation in TNF-alpha, suggesting a complex interaction between protein kinase C and protein kinase A in cytokine regulation. (ox.ac.uk)

Notably, the nonphosphorylatable mutant (T739A), aswell as the various other neuroligin isoforms that people put through the same kinase assay, demonstrated no immunoreactivity with pT739-Ab, highlighting the specificity of pT739-Ab for NL-1 phosphorylated at T739. (findadig.com)

Evidence is accumulating that small G proteins are involved in the regulation of cell growth and differentiation. (nii.ac.jp)
Noncanonical regulation of phosphatidylserine metabolism by a Sec14-like protein and a lipid kinase. (tamu.edu)
Here we outline the current status of the viral evasion mechanism underlying the regulation of the cGAS-STING pathway, focusing on how post-transcriptional modifications, viral proteins, and non-coding RNAs involve innate immunity during viral infection, attempting to inspire new targets discovery and uncover potential clinical antiviral treatments. (frontiersin.org)
Synapsins are well established targets for multiple protein kinases within the nerve terminal, yet little is known about dephosphorylation processes involved in regulation of synapsin function. (ucl.ac.uk)

Codina J, Stengel D, Woo SL, Birnbaumer L: Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical. (smpdb.ca)

Activator of protein kinase A (cyclic AMP agonist). (biolog.de)

The phosphorylated proteins may act directly on the cell's ion channels, or may become activated or inhibited enzymes. (wikipedia.org)

Cyclic AMP is synthesized from ATP by adenylate cyclase located on the inner side of the plasma membrane and anchored at various locations in the interior of the cell. (wikipedia.org)

The active subunits catalyze the transfer of phosphate from ATP to specific serine or threonine residues of protein substrates. (wikipedia.org)
We have found that the C-terminal systeine residues of smg p21B, rhoA p21, and smg p25A are geranylgeranylated, and that these prenylation are essential for each small G protein to bind to membranes. (nii.ac.jp)

These are termed Exchange proteins activated by cAMP (Epac) and the family comprises Epac1 and Epac2 . (wikipedia.org)

They are tetrameric proteins that contain two catalytic subunits and two type II-specific regulatory subunits. (uchicago.edu)
In this research project, we have investigated the C-terminal structures of small G proteins, purified the regulatory proteins for small G proteins, and made monoclonal antibodies against small G proteins and their regulatory proteins. (nii.ac.jp)
Among these regulatory proteins, we have cloned the cDNAs of smg p21 GDS, smg p25A GDI, and rho GDI, and made monoclonal antibodies against them. (nii.ac.jp)
AKAP350 is a large protein that can potentially assemble large regulatory compress within the cell. (grantome.com)

a) Immunoblot evaluation with pT739-Ab of GST, GSTCNL-1 (outrageous type or T739A), GSTCNL-2, GSTCNL-4 and GSTCNL-3 which were phosphorylated with purified catalytic subunits of CaMKII. (findadig.com)

In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1). (nih.gov)
Common genetic variants in the glucocorticoid receptor and the 11ß-hydroxysteroid dehydrogenase type 1 genes influence long-term cognitive impairments in patients with Cushing's syndrome in remission. (cdc.gov)

Luciferase reporter assays demonstrated that the Adcy1 promoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1. (jneurosci.org)

Mitochondrial membrane biogenesis: phospholipids and proteins go hand in hand. (tamu.edu)

Publications] Araki,S.: 'Role of the Cーterminal region of smg p25A in its interaction with membranes and the GDP/GTP exchange protein. (nii.ac.jp)

These findings suggest that the anti-atrophic effect of clenbuterol may be dependent on the upregulation of TRPC3 through the activation of the PLC-ß1/DAG pathway during mechanical unloading. (bvsalud.org)

A cyclic AMP-dependent protein kinase subtype primarily found in particulate subcellular fractions. (uchicago.edu)
While we and others have demonstrated that AKAP350 can potentially scaffold a wide range of proteins, the actual composition of scaffolded complexes is likely both cell specific as well as subcellular organelle specific. (grantome.com)

In addition, preincubation of extracts with cAMP-dependent protein kinase (PKA) blocks stimulation of MAPK by cyclin, p21V12ras, PKC, or pp60v-src, by at least 50%, but stimulation by c-Mos is unaffected. (ox.ac.uk)

The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. (wikipedia.org)

Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. (wikipedia.org)
Our previous studies have suggested that two phosphodiesterase type 10A (PDE10A) variants, PDE10A1 and PDE10A2 transcripts, are mainly expressed in humans and that PDE10A2 and PDE10A3 transcripts are major variants in rats. (johnshopkins.edu)

For instance, due to their genetic flexibility, viruses have developed various viral proteins and non-coding RNAs to interrupt several checkpoints of cGAS-STING. (frontiersin.org)

We demonstrate here that the kinases Gsk3β and cAMP-dependent protein kinase A (PKA) are novel determinants of IZ formation and cooperate with E(Spl) activity in a dose-dependent manner. (biologists.com)
We demonstrate that this disparity depends on differences in the rates of phosphatase-dependent dephosphorylation of PKA targets in the two compartments. (ox.ac.uk)

Publications] Sasaki,T.: 'A mammalian inhibitory GDP/GTP exchange protein(GDI)for smg p25A is active on the yeast SEC4 protein. (nii.ac.jp)

Anatomy19

Organisms13

Chemicals and Drugs163

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes45

Information Science3

Subunit7

Phosphorylation7

Phosphorylated by cAMP-depend1

Adenosine6

Phosphodiesterases4

Receptors8

Calcium3

Pathways5

Guanine nucleo1

Inhibitor6

Phosphatases3

Activates4

Mitogen-activat3

Nucleotides1

Cells15

MeSH1

Inhibition3

Glycogen4

Phosphorylate3

Cytokine3

Isoforms1

Regulation4

Bovine1

Activator1

Enzymes1

Adenylate cyclase1

Residues2

Termed Exchange proteins1

Regulatory4

Catalytic subunits1

Genes2

Neuronal1

Mitochondrial1

Membranes1

Upregulation1

Subcellular2

Stimulation1

Transcription1

Humans2

RNAs1

Demonstrate2

Inhibitory1