Mutagenesis, Site-DirectedKineticsCatalysisMolecular Sequence DataAmino Acid SequenceBinding SitesMutationEscherichia coliSubstrate SpecificityHistidineRecombinant ProteinsEnzyme StabilityBase SequenceModels, MolecularCysteineAmino Acid SubstitutionCatalytic DomainStructure-Activity RelationshipHydrogen-Ion ConcentrationProtein ConformationLysineOligodeoxyribonucleotidesAspartic AcidSequence Homology, Amino AcidCircular DichroismPoint MutationHydrolysisSpectrophotometryOxidation-ReductionCloning, MolecularPlasmidsCrystallography, X-RayArginineDNA PrimersElectrophoresis, Polyacrylamide GelProtein EngineeringRestriction MappingSerineHydrogen BondingSaccharomyces cerevisiaeProtein Structure, SecondaryProtein Structure, TertiarySpectrometry, FluorescenceTyrosineMutagenesisProtein BindingAdenosine TriphosphateMacromolecular SubstancesThermodynamicsBacterial ProteinsModels, ChemicalMolecular WeightEnzyme ActivationIsoenzymesDNASequence AlignmentTemperatureCell Line