Two antifreeze proteins with molecular. Medical search. Frequent questions

Antifreeze glycoproteins or AFGPs are found in Antarctic notothenioids and northern cod. (wikipedia.org)
For example, these fish have evolved special "antifreeze" glycoproteins that prevent the formation of ice in their cells. (phys.org)
These changes are predicted to have occurred during two key periods in Antarctic icefish history: the evolution of antifreeze glycoproteins and the onset of freezing polar conditions. (phys.org)

Dr. Gaurav Chopra and Professor Michael Levitt in the Department of Structural Biology used molecular dynamics simulations involving a state-of-the-art Quantum Mechanical Polarizable Force Field (QMPFF3) to study the hydration of buckminsterfullerene, the smallest hydrophobic nanosphere widely referred to as a buckyball or C 60 . (phys.org)
Molecular Biology and Evolution (2023). (phys.org)
Adding to this repertoire of changes, a recent study published in Molecular Biology and Evolution reveals the genetic mechanisms by which the visual systems of Antarctic icefishes have adapted to both the extreme cold and the unique lighting conditions under Antarctic sea ice. (phys.org)
Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of proteins, making them attractive candidates as concept generators for technological development in material science, particularly in biomedicine and nano(bio)technology. (uni-bayreuth.de)
Computational design of novel proteins with well-defined functions is an ongoing topic in computational biology. (hindawi.com)
We are studying the adaptations of marine species in order to understand their biology at the molecular level while also shining light on universal processes important in human health. (dal.ca)
Due to the increasing investments towards the research in molecular biology, this market is expected to increase and also due to the diverse application of antifreeze protein market. (fortunebusinessinsights.com)
This chapter begins with a historical review of the scientific contributions to plant antifreeze protein biology made by Dr. Marilyn Griffith. (canada.ca)
1. When ID supporters ask for evidence of macroevolution, biologists point to the fossil record, molecular biology and comparative anatomy to make their case. (uncommondescent.com)
2. If a direct demonstration of macroevolution is not possible, then what about the indirect evidence of the fossil record, comparative anatomy and molecular biology? (uncommondescent.com)
It encompasses developmental biology , organismal biology, molecular evolution , and experimental evolution . (freescience.info)
She also holds a B.S. in molecular biology and an M.S. in biology from the University of California, San Diego. (livescience.com)

Beetle hyperactive antifreeze protein (AFP) has a unique ability to maintain a supercooling state of its body fluids, however, less is known about its origination. (mdpi.com)
High water mobility on the ice-binding surface of a hyperactive antifreeze protein. (lu.se)
A mechanism of interactions between the ice-binding surface of a hyperactive antifreeze protein molecule and the ice surface is proposed, involving the influence of water present between the two surfaces on the behavior of the approaching molecule. (mostwiedzy.pl)

The protein was isolated from the blood plasma of winter flounder. (wikipedia.org)
Conversion of a winter flounder antifreeze protein from an alpha helix to an amyloid conformation can be quickly triggered by freezing and thawing. (dal.ca)
Dubé, A., Leggiadro, C. and Ewart, K.V. , (2016) Rapid amyloid fibril formation by a winter flounder antifreeze protein requires specific interaction with ice. (dal.ca)
We present the X-ray crystal structure at 1.5 Å resolution of a lone a-helical antifreeze protein from winter flounder, which provides a detailed look at its ice-binding features. (tue.nl)
Sicheri, F & Yang, DSC 1995, ' Ice-binding structure and mechanism of an antifreeze protein from winter flounder ', Nature , vol. 375, pp. 427-431. (tue.nl)

Abstract] Poly(vinyl alcohol) (PVA) displays ice recrystallization inhibition (IRI) properties as many antifreeze proteins found in cold tolerant organisms. (udc.es)

The in-vitro transcribed gene induced an antifreeze protein with a molecular size of 22 kDa. (bvsalud.org)
The antifreeze gene, ITGA-2 and the WD repeat protein belong to the lectin family (sugar-protein). (bvsalud.org)
CONCLUSIONS: In conclusion, under cold stress, Nile tilapia express many defence genes, an antifreeze gene consisting of one open reading frame of approximately 0.6 kbp. (bvsalud.org)
It is a difficult question because, setting aside many other problems, the very starting point - the protein-coding gene - is highly complex. (evolutionnews.org)
But this week researchers at the University of Illinois announced ground-breaking research that provides a step-by-step, detailed, description of the evolution of a new protein-coding gene and associated regulatory DNA sequences. (evolutionnews.org)
The protein in question is a so-called "antifreeze" protein that keeps the blood of Arctic codfish from freezing, and the new research provides the specific sequence of mutations, leading to the new gene. (evolutionnews.org)
As the paper triumphantly declares, "Here, we report clear evidence and a detailed molecular mechanism for the de novo formation of the northern gadid (codfish) antifreeze glycoprotein (AFGP) gene from a minimal noncoding sequence. (evolutionnews.org)
Having the scientific details, down to the level of specific mutations, of how a new protein-coding gene evolved - not from a related gene but from non-coding DNA - is something evolutionists could only dream of only a few short years ago. (evolutionnews.org)
For example, the antifreeze protein is of relatively low complexity chiefly consisting of a repeating sequence of three amino acids (threonine-alanine-alanine), and the evolutionists claim that these repeating sequences "strongly suggest" that the protein-coding gene "evolved from repeated duplications of an ancestral 9-nucleotide threonine-alanine-alanine-coding element. (evolutionnews.org)
The ALK gene is a hotspot for chromosomal translocation events that result in several fusion proteins that cause a variety of human malignancies 3 . (nature.com)
Fusion of a kinase gene, ALK , to a nucleolar protein gene, NPM , in non-Hodgkin's lymphoma. (nature.com)
Transmembrane protein 131 (TMEM131) is a protein that is encoded by the TMEM131 gene in humans. (wn.com)
The diverse fish antifreeze protein (AFP) provides a unique system for studying new gene and novel function formation, as they have reasonably recent origins, and their lifesaving function of preventing freezing is clearly defined and can be directly linked to natural selection. (uark.edu)
We identified different precursor genes in different lineages and will further deduce the evolutionary process and molecular mechanism of the new gene birth. (uark.edu)

Time scales of water dynamics at biological interfaces: peptides, proteins and cells. (lu.se)
Antifreeze peptides can protect cell membranes and maintain the cell viability of probiotics under cold stress. (bioinfor.com)
Given this, antifreeze peptides were prepared from tilapia processing byproducts of tilapia skin by enzymolysis using the response surface methodology (RSM) method. (bioinfor.com)
Molecular weight ( M w ) distribution and peptide sequences of the antifreeze peptides prepared from tilapia skin (APT) under the optimal conditions were analyzed. (bioinfor.com)

Antifreeze proteins have been known for over five decades, however, their exact mechanism of action is still under investigation. (unh.edu)
HR-MAS NMR proved to be a viable tool for the elucidation of the molecular mechanism of ionic liquid - protein interactions. (unh.edu)
Molecular mechanism regulating axoneme activation in marine fish: a review. (upv.es)
A mechanism for stabilization of membranes at low temperatures by an antifreeze protein. (mpg.de)

The present study was carried out to investigate the effects of cold stress on the up-regulation of antifreeze protein (AFP) genes in Nile tilapia (Oreochromis niloticus). (bvsalud.org)
These genes are Integrin-alpha-2 (ITGA-2), Gap junction gamma-1 protein-like (GJC1), WD repeat-containing protein 59 isoform X2 (WDRP59), NUAK family SNF1-like kinase, G-protein coupled receptor-176 (GPR-176), Actin cytoskeleton-regulatory complex protein pan1-like (PAN-1), Whirlin protein (WHRN), Suppressor of tumorigenicity 7 protein isoform X2 (ST7P) and ATP-binding cassette sub-family A member 1-like isoform X2 (ABCA1). (bvsalud.org)
Three CBF genes that are responsive to cold were identified from Brachypodium , designated BdCBF1 , BdCBF2 , and BdCBF3 , and they were functionally characterized by molecular biological and transgenic approaches in Brachypodium and Arabidopsis thaliana . (biomedcentral.com)
The BdCBF proteins possess transcriptional activation activity and bind directly to the promoters of the target genes. (biomedcentral.com)

F.D. Sönnichsen, J.E. Van Eyk, R.S. Hodges, B.D. Sykes, 'Effect of Trifluoroethanol on Protein Secondary Structure: An NMR and CD Study using a Synthetic Actin Peptide', Biochemistry 31, 8790-8798. (uni-kiel.de)

Interestingly, the amino acid changes observed in the Antarctic icefishes were distinct from those conferring cold adaptation in the high-altitude catfishes previously studied by the team, suggesting multiple pathways to adaptation in this protein. (phys.org)
MEDLINE_UI_code 99353969 J Biochem (Tokyo) 1999 Aug;126(2):387-94 Determination of the solution structure of the N-domain plus linker of antarctic eel pout antifreeze protein RD3. (bmrb.io)
Citation.Title 'Determination of the solution structure of the N-domain plus linker of Antarctic eel pout antifreeze protein RD3. (bmrb.io)
RD3, a new antifreeze protein (AFP) extracted from antarctic eel pout is a single polypeptide divided into homologous N-terminal (residues Asn(1)-Glu(64)) and C-terminal (residues Ser(74)-Glu(134)) domains, each of which has a high sequence identity with Type III AFP. (bmrb.io)

These intriguing proteins are found in a number of cold-environment organisms such as fish, insects, plants and bacteria. (uoguelph.ca)
Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. (uni-bayreuth.de)
Neutron scattering : a tool to detect in vivo thermal stress effects at the molecular dynamics level in micro-organisms. (ibs.fr)
Antifreeze Proteins (AFPs) or Ice Structuring Proteins (ISPs) refers to polypeptides, generally produced by certain types of organisms such as fungi, bacteria, insects, and animals. (fortunebusinessinsights.com)
In March 2019, A German-Israeli research team started a clinical study in order to understand, how the antifreeze proteins prevent the certain organisms from freezing. (fortunebusinessinsights.com)

The power to 'see' proteins at the atomic level amazed me, and gave me the tools to understand protein interactions at a very fundamental level. (uoguelph.ca)
NMR also lends itself to the detailed study of protein/ligand interactions, and is able to characterize proteins both in solution and in a solid such as ice or membranes. (uoguelph.ca)
Exploration of the supramolecular interactions involving tris-dipicolinate lanthanide complexes in protein crystals by a combined biostructural, computational and NMR study. (ibs.fr)
Ionic liquids are highly viscous liquids at room temperature comprised entirely of ions, which makes it challenging to study their interactions with protein by traditional NMR methods. (unh.edu)
Hydrophobicity was one of the most important physicochemical properties, due to the fact that it is involved in protein interactions, for example, by forming hydrophobic cores. (hindawi.com)

I began to appreciate that complex biological process can be understood in terms of the interaction of proteins and other biological molecules, an idea which still intrigues me to this day. (uoguelph.ca)
NMR is an extremely powerful technique for understanding protein structure and function, especially since it is able to characterize the flexibility of biological molecules. (uoguelph.ca)
For this reason, QMPFF3 is expected to have a profound effect in understanding key biological processes like protein folding. (phys.org)
In convergent evolution, nonhomologous proteins evolve in separate biological contexts to catalyze the same or similar reactions. (hindawi.com)
A transmembrane protein (TP) is a type of membrane protein spanning the entirety of the biological membrane to which it is permanently attached . (wn.com)

During my graduate studies, I studied diverse protein/ligand systems, including amyloid (Alzheimer) proteins interacting with basement membrane proteins, and the role of calmodulin and caldesmon in smooth muscle regulation. (uoguelph.ca)
Change of dynamics of raft-model membrane induced by amyloid-beta protein binding. (ibs.fr)
In contrast, human disease-relevant amyloid-forming proteins tend to require long time intervals (hours to weeks) to form amyloid, which slows research. (dal.ca)
Therefore, we are evaluating the process of rapid amyloid formation by this antifreeze protein. (dal.ca)

Because of these proteins' ability to prevent and slow the rate of ice crystal formation, they have great potential in the application of cryopreservation in medicine, agriculture and food science. (unh.edu)
Effect of cryopreservation on sea bass sperm proteins. (upv.es)
Effect of cryopreservation on phosphorylation state of proteins involved in sperm motility initiation in sea bream. (upv.es)
Improving Sperm Cryopreservation with Antifreeze Proteins: Effect on Gilthead Seabream (Sparus aurata) Plasma Membrane Lipids. (upv.es)

Our group is interested in understanding how antifreeze protein binds to its ligand. (uoguelph.ca)
When Ca2+ channels are activated in response to a variety stimuli, a drastic increase in the [Ca2+]c is induced and the entered free Ca2+ binds to the sets of Ca2+-regulated proteins such as calmodulin and calcium dependent protein kinases to modify the activities or affinities of these proteins in binding to specific targets. (globalsciencebooks.info)

Antifreeze proteins (AFPs) or ice structuring proteins refer to a class of polypeptides produced by certain animals, plants, fungi and bacteria that permit their survival in temperatures below the freezing point of water. (wikipedia.org)
Unlike the widely used automotive antifreeze, ethylene glycol, AFPs do not lower freezing point in proportion to concentration. (wikipedia.org)
Antifreeze proteins (AFPs) are a unique class of protein characterized by their ability to depress the freezing point of water sufficient to prevent the formation of ice crystals by adsorbing to the surface of ice crystals. (unh.edu)

There were many challenges to overcome in designing and implementing QMPFF3-based molecular dynamics simulations especially to study the behavior of water molecules next to hydrophobic surfaces at atomic detail and subpicosecond time resolution. (phys.org)
Analysis of protein dynamics simulations by a stochastic point process approach. (lu.se)
First, the sequences carrying point mutations are modeled on a given template structure, and subsequently, molecular dynamics simulations are carried out for 10 ns. (hindawi.com)
The limits of current methods is the incorporation of molecular dynamics simulations into the multi-objective optimization. (hindawi.com)
Due to the fact that molecular dynamics simulations are very expensive regarding computational time, new fitness functions have to be introduced without loosing predictive power. (hindawi.com)

Having a precise description of water s structure is critical to constructing accurate simulations of molecular events, including protein folding, substrate binding, macromolecular recognition, and complex formation. (phys.org)
molecular simulations. (lu.se)

Though the formation of clathrates around hydrophobic groups is the main phenomenon that drives binding, it is still unclear what features allow these clathrates to form on antifreeze protein surfaces but not on other proteins. (uoguelph.ca)
F.D. Sönnichsen, C.I. DeLuca, P.L. Davies, B.D. Sykes, 'Refined solution structure of Type III Antifreeze protein: hydrophobic groups may be involved in the energetics of the protein-ice interaction', Structure 4 (15), 1325-1337. (uni-kiel.de)

As a result of ice binding, antifreeze proteins can depress of the solution freezing point, modulate the ice nucleation temperature and alter of the tendency of ice to coalesce through recrystallization. (dal.ca)

These proteins are expressed in plants during times of desiccative stress, and are thought to bind water, protect the cellular membrane from drying and prevent proteins from denaturing. (uoguelph.ca)
Structure of an Intrinsically Disordered Stress Protein Alone and Bound to a Membrane Surface. (uoguelph.ca)
Small angle neutron scattering for the study of solubilised membrane proteins. (ibs.fr)
This process, known as hardening, results in a set of physiological changes at the molecular level that initiate modifications of cell membrane composition and the synthesis of anti-freeze proteins. (copernicus.org)
A yeast-based model system for cloning secreted and membrane proteins. (mpg.de)
That is, transmembrane proteins span from one side of a membrane through to the other side of the membrane. (wn.com)
All transmembrane proteins are integral membrane proteins (IMPs), but not all IMPs are transmembrane proteins. (wn.com)

IDPs are fascinating proteins since they often fail to follow biochemical dogma (lack of structure, high specificity in the absence of high affinity). (uoguelph.ca)
Production of novel new molecular machines or biochemical pathways - with one or two possible exceptions like antifreeze proteins, which Behe discusses - appears beyond its abilities irrespective of time. (grisda.org)

We found the protein to have a β-helix secondary structure, similar to other known insect antifreeze proteins. (unh.edu)
The optimization was guided by directed evolution based on hydrophobicity scores, molecular weight, and secondary structure predictions. (hindawi.com)

Storage protein degradation is induced by cold temperature, and the accumulation of a soluble N fraction, especially glutamine, occurs. (globalsciencebooks.info)

Together with the freezing of extracellular water, anti-freeze proteins reduce plant water potentials and xylem conductivity. (copernicus.org)
APT significantly reduced the leakage of extracellular proteins and protected β -galactosidase and lactate dehydrogenase activities of L. rhamnosus . (bioinfor.com)

Protein design methods use trial and error or more sophisticated methods like directed evolution or inverse folding to generate novel scaffolds or to find novel protein sequences folding into a defined scaffold, respectively. (hindawi.com)
Given the intimate relationship between a protein's structure and function, a way to design proteins with targeted properties is to start from a desired structure and find sequences able to fold into it, imposing additional constraints in the process [ 1 ]. (hindawi.com)

More than 400 million years ago, an insect-like animal called the springtail developed a small protein that prevents its cells from freezing. (yubanet.com)
In this study, we have characterized the antifreeze protein ApAFP752 from the beetle Anatolica polita to better understand method of action of antifreeze activity of insect antifreeze proteins and to identify it potential applications in cryoprotection. (unh.edu)
At present, insect accounts for the largest proportion of the market, because a number of antifreeze proteins products are derived or sourced from the insects. (fortunebusinessinsights.com)

Some icefishes are "white-blooded" due to no longer making hemoglobin, and some have lost the inducible heat shock response, a nearly universal molecular response to high temperatures. (phys.org)
Antifreeze proteins have been proposed to protect cells at cold temperatures above the freezing point, where no ice is present. (dal.ca)

We knew that antifreeze proteins had developed independently of each other several times during evolutionary history. (yubanet.com)

Nanosecond to microsecond protein dynamics probed by magnetic relaxation dispersion of buried water molecules. (lu.se)
With Reactions bestselling author Theodore Gray continues the journey through our molecular and chemical world that began with the tour de force The Elements and continued with Molecules. (stanford.edu)

The absence of a thymine nucleotide at a location in some of the species "very likely resulted from a deletion event," causing a fortuitous frameshift which supplied the crucial signal peptide segment, telling cellular machinery that the protein should be secreted to the bloodstream. (evolutionnews.org)

A large number of random mutations would seem to be required before you have a functional protein that helps the organism. (evolutionnews.org)

Protein cold denaturation as seen from the solvent. (lu.se)

At Last, the Details of How Proteins Evolve? (evolutionnews.org)
How did proteins evolve? (evolutionnews.org)

Aquaporin inhibition changes protein phosphorylation pattern following sperm motility activation in fish. (upv.es)

In this work, we generated and optimized a new synthetic fusion protein using an evolutionary approach. (hindawi.com)

2022. "Grain protein concentration at elevated [CO2] is determined by genotype dependent variations in nitrogen remobilisation and nitrogen utilisation efficiency in wheat. (edu.au)

This phenomenon allows them to act as an antifreeze at concentrations 1/300th to 1/500th of those of other dissolved solutes. (wikipedia.org)

Then the current state of knowledge on this topic is outlined, focusing on recent advances in understanding the roles of these proteins as modulators of freezing and ice recrystallization, and the mechanisms by which they function at the molecular level. (canada.ca)

Additionally, the protein was found to confer some cryoprotection to cellular systems. (unh.edu)

It is demonstrated that the interfacial water, even before its full solidification, can act as a factor that pushes away or pulls nearer the protein molecule to ensure its proper positioning. (mostwiedzy.pl)

Combining small-angle neutron and X-ray scattering for studying protein denaturation. (ibs.fr)

But the breakthrough came when I joined a crystallography lab and began to look at how antifreeze proteins can interact with ice crystals. (uoguelph.ca)
Antifreeze proteins provide fish with protection against the freezing effect of polar environments by binding to ice surfaces and inhibiting growth of ice crystals. (tue.nl)

Some of the medical applications of antifreeze proteins include cryosurgery, transplantation procedures, and hypothermia therapeutics. (fortunebusinessinsights.com)
The growth in the number of applications of antifreeze proteins and their critical applications in the procedures such as transplantation, cryosurgery and hypothermia therapeutics are one of the main reasons for the growth of the market. (fortunebusinessinsights.com)

We have also investigated the effects of ionic liquids on protein biophysics. (unh.edu)

We propose an ice-binding model that accounts for the binding specificity of the antifreeze protein along the axes of the {2021} ice planes. (tue.nl)

Her Analytical chemistry study combines topics in areas such as Chemical physics, Guanidinium Cation, Cytochrome c, Trehalose and Molecular physics. (research.com)

Cryoprotection, a cabbage protein protecting thylakoids from freeze-thraw damage. (mpg.de)

The edge of evolution - the limit to what mutation coupled with selection can achieve - seems to be restricted to mutations that disrupt to varying degrees the normal function of already existing proteins. (grisda.org)

Her work on Molecular vibration as part of general Analytical chemistry research is frequently linked to Freezing behavior, thereby connecting diverse disciplines of science. (research.com)

The geometry of protein hydration. (lu.se)
The spatial range of protein hydration. (lu.se)
Hydration dynamics of a halophilic protein in folded and unfolded states. (lu.se)
Does the dynamic Stokes shift report on slow protein hydration dynamics? (lu.se)

This domain has not been found in proteins other than TMEM53 and its orthologs. (wn.com)
Scientists may have found a new way to reduce levels of a toxic protein that accumulates in the brains of Alzheimer's patients. (livescience.com)

Combining NMR and small angle X-ray and neutron scattering in the structural analysis of a ternary protein-RNA complex. (ibs.fr)

Transmembrane proteins are polytopic proteins that aggregate and precipitate in water. (wn.com)

The ecology of protein dynamics. (ibs.fr)

However, they have not been functionally characterized at the molecular level. (biomedcentral.com)

the fish were acclimated to lab conditions for two weeks and the water temperature was maintained at 25 °C during the whole experimental period with feeding on a commercial diet (30% crude protein). (bvsalud.org)

In a crankcase-lubricated engine, the oil compartment acts as a sink for heavy molecular incomplete combustion products such as PAHs and their analogs (Scheepers and Bos 1992). (cdc.gov)

In this study, the effect of an ionic liquid (1-butyl-3-methylimidazolium bromide, [C4-mim]Br) on the structure and dynamics of the model protein GB1 was investigated using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy. (unh.edu)

These proteins are both tissue- and species-specific. (bvsalud.org)

Compressibility of the protein-water interface. (lu.se)
How proteins modify water dynamics. (lu.se)
Slow internal protein dynamics from water 1 H magnetic relaxation dispersion. (lu.se)
Dynamics at the protein-water interface from 17 O spin relaxation in deeply supercooled solutions. (lu.se)
In addition, a variety of substances such as fuel, water, antifreeze, dust, and various combustion products such as polycyclic aromatic hydrocarbons (PAHs), metals, and metallic oxides accumulate in the oil. (cdc.gov)

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