SnailBHLH transcription factorTWIST1ZEB1ProteinProteinsTranscriptionalMetastasisDrosophilaDownstreamRegulateFibroblast growthEpidermalDifferentiationOverexpressionCellularPromoterProliferationRegulationMotifsInhibitionTumor cellsEGFREndothelialCloselyCancerDiagnosticNuclearGeneticTimeInteractRisk factorsCell
Snail9
- The results demonstrated that PPII treatment significantly inhibited cancer cell migration and invasion, increased the expression level of E‑cadherin and decreased the levels of N‑cadherin, snail family transcriptional repressor 2, twist family bHLH transcription factor 1, matrix metallopeptidase (MMP) 2 and MMP9 compared with those in the control group (untreated cells). (spandidos-publications.com)
- It is dynamically orchestrated by transcription factors (EMT-TFs), including Snail, Zeb, Twist and Prrx, all activated by TGF-β among other signals. (researchgate.net)
- Several lines of evidence support an important role for Snail, a transcriptional factor, in breast cancer. (spandidos-publications.com)
- Studies have shown that several transcription factors, including the Snail/Slug family ( 3 ), δEF1/ZEB1 ( 4 ) and SIP1 ( 5 ) have been implicated in E-cadherin repression. (spandidos-publications.com)
- We hypothesized that Snail, as a transcription factor, may promote breast cancer metastasis through the regulation of RhoA expression and activity. (spandidos-publications.com)
- EMT is a multistep process involved in cancer metastasis, mediated by activation of Slug/Snail, Twist and/or Zeb transcription factors (TFs), and characterized by a specific gene signature. (life-science-alliance.org)
- The link between these two phenomena is still not elucidated, although several important molecules involved in both these complex processes, such as transcription factors (SNAIL, TWIST, ZEB, etc.) and miRNAs (miRNA-200 family, miR-15, miR-186, etc.) have been recognized as important. (irb.hr)
- [ 4 ] EMT programs are orchestrated by a set of pleiotropically acting transcription factors, including Slug, Snail, Twist, ZEB1, and ZEB2, which organize entrance into a mesenchymal state by suppressing expression of epithelial markers (E-cadherin) and inducing expression of other markers associated with the mesenchymal state. (medscape.com)
- The change in morphology is accompanied by molecular change initiated by several pathways and signalling factors which regulate expression of transcription factors (EMT-TFs), including Snail, ZEB, Twist and others 6 . (lungdiseasesjournal.com)
BHLH transcription factor3
- This gene encodes for a bHLH transcription factor, which inhibits osteogenic differentiation by transc. (uni-marburg.de)
- The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1 (a.k.a. (wikipedia.org)
- The bHLH transcription factor TWIST1 plays a key role in the embryonic development and in tumorigenesis. (biomedcentral.com)
TWIST15
- Using the entire coding sequence of TWIST1 gene, an interesting candidate was found belonging to the class A bHLH transcription factors that includes the SEF2 gene product. (uni-marburg.de)
- Furthermore, two more highly conserved TWIST motifs NSEEE and WR were analyzed individually to find out their interacting proteins and their role in regulating the TWIST1 transcriptional activity. (uni-marburg.de)
- Twist-related protein 1 (TWIST1) also known as class A basic helix-loop-helix protein 38 (bHLHa38) is a basic helix-loop-helix transcription factor that in humans is encoded by the TWIST1 gene. (wikipedia.org)
- Although the C-terminal sequences adjacent to the bHLH domain, constitute a protein interaction site essential for TWIST function [4] [5] , we reasoned that a structural model restricted to the bHLH domains should provide additional insights into the TWIST-DNA interface and highlight functional differences between TWIST1 complexes. (proteopedia.org)
- TWIST1 is a transcription factor belonging to the basic helix-loop-helix (bHLH) superfamily. (biomedcentral.com)
ZEB11
- Other patients show evidences of epithelial-mesenchymal transition (EMT) activation, a transcriptional embryonic program characterized by the fibroblast-like morphology, the repression of epithelial cadherin (CDH1) and the expression of mesenchymal markers (such as vimentin, N-cadherin) and transcription factors ZEB1, SNAIL or TWIST). (uv.es)
Protein12
- The aim of my work is to characterize functional domains in TWIST protein, and to determine the interacting partner for TWIST and its motifs particularly NSEEE and WR. (uni-marburg.de)
- This synergistic effect is consistent with the observation that combined K38R (NLS1) and K76R (NLS2) mutants dramatically reduced nuclear localization, further suggesting that both NLS1 and NLS2 work together in regulating nuclear localization of TWIST protein. (uni-marburg.de)
- Accordingly, the subcellular localization of NLS1 and NLS2 in TWIST protein was investigated in U2-OS cells following co-transfection with E12. (uni-marburg.de)
- I wanted to determine if the TWIST protein or its conserved motifs interact with other regulatory proteins to help to regulate the TWIST transcriptional activity. (uni-marburg.de)
- The direct interaction of SEF2 with TWIST was verified in a yeast mating assay and then confirmed in an in vivo NLS-rescue assay using U2-OS cells, showing that SEF2 forms a heterodimer with TWIST protein and co-localized into the nucleus. (uni-marburg.de)
- A mechanistic analysis reveals that Ubx requires the NK- homeodomain protein Tinman to bind to the twist promoter. (bvsalud.org)
- Furthermore, we find these factor interactions to be critical for silencing by recruiting the Polycomb DNA binding protein Pleiohomeotic. (bvsalud.org)
- Twist is activated by a variety of signal transduction pathways, including Akt, signal transducer and activator of transcription 3 (STAT3), mitogen-activated protein kinase, Ras, and Wnt signaling. (wikipedia.org)
- Western blotting was performed to detect the protein levels of TWIST and EMT-related genes. (hindawi.com)
- Over 160 TWIST mutations have been described in SCS patients, a majority of which are present in the bHLH domains, mediating protein dimerization, and involving a single base pair substitution (54% of mutations). (biomedcentral.com)
- The mesenchymal protein transcription factor Twist can bind to autophagy related protein p62 and promote the degradation of Twist, which reduced the expression of mesenchymal markers. (ijbs.com)
- The protein produced from the FOXF1 gene is a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity of many other genes. (medlineplus.gov)
Proteins4
- Evolutionary alignment of Twist proteins from different species, indicate TWIST contain 4 additional conserved regions such as NSEEE, NLS1, NLS2, and WR-domain besides the bHLH motif. (uni-marburg.de)
- TWIST belongs to class B bHLH proteins which are known to form stable heterodimers with members of class A bHLH transcription factors including gene products of E12 and E47, respectively. (uni-marburg.de)
- The second aim of my work was to identify the interacting proteins that could influence the functionality of TWIST using yeast-two-hybrid assay. (uni-marburg.de)
- Rho GTPases represent a family of small GTP-binding proteins involved in cell cytoskeleton organization, migration, transcription, and proliferation. (biomedcentral.com)
Transcriptional1
- We find that early transcriptional patterning of key morphogenetic transcription factors drives the selective activation of downstream morphogenetic modules, such as GPCR signaling that activates apical-medial actomyosin activity to drive cell wedging at the future asymmetrically placed invagination point. (elifesciences.org)
Metastasis4
- It can present in nonsyndromic forms (isolated scaphocephaly, right unicoronal synostosis, and turricephaly), but also in syndromic forms such as: Acrocephalosyndactyly type 1 (Apert syndrome) (primary FGFR2) Beare-Stevenson cutis gyrata syndrome (primary FGFR2) Crouzon syndrome (primary FGFR2) Crouzon syndrome-acanthosis nigricans syndrome (primary FGFR3) Jackson-Weiss syndrome (primary FGFR1 or FGFR2) Muenke syndrome (primary FGFR3) Pfeiffer syndrome (primary FGFR1 or FGFR2) Twist plays an essential role in cancer metastasis. (wikipedia.org)
- Another process in which Twist 1 is involved is tumour metastasis. (wikipedia.org)
- TIP60-dependent acetylation of the SPZ1-TWIST complex promotes epithelial-mesenchymal transition and metastasis in liver cancer. (kmu.edu.tw)
- In addition, gene expression analysis shows that E-cadherin loss results in the induction of multiple transcription factors, at least one of which, Twist, is necessary for E-cadherin loss-induced metastasis. (gsea-msigdb.org)
Drosophila2
- Using the Drosophila mesoderm as a model and a comparative genomic approach, we identify the Hox transcription factor Ultrabithorax (Ubx) to be critical for the repression of the master regulator Twist. (bvsalud.org)
- By correlating dynamic patterns of transcription factor expression with rigorous, quantitative analyses of cell behaviors across the salivary gland primordium in Drosophila , their results suggest Hkb and Fkh transcription factor patterning induces switches in cell behaviors at fixed positions to promote continued morphogenesis of the tubular structure. (elifesciences.org)
Downstream3
- Moreover, several inhibitors which are antagonistic to the upstream or downstream molecules of Twist signaling pathways have also been identified. (wikipedia.org)
- Our work illustrates that the dynamic patterning of the expression of transcription factors and downstream morphogenetic effectors ensures positionally fixed areas of cell behavior with regards to the invagination point. (elifesciences.org)
- The exchange of GDP to GTP and thus the activation of Rho GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), which act downstream of numerous growth factor receptors, integrins, cytokine receptors, and cadherins. (biomedcentral.com)
Regulate3
- PYK2 and PKCα cooperate at a convergence point of multiple stemness-inducing pathways to regulate AXL levels and concomitantly the levels/activation of STAT3, TAZ, FRA1, and SMAD3 as well as the pluripotent transcription factors Nanog and Oct4. (life-science-alliance.org)
- Pleotropic signalling factors such as transforming growth factor β (TGFβ), fibroblast growth factor (FGF), Wnt/β-catenin and epidermal growth factor (EGF) can initiate EMT, in turn, these factors regulate expression of EMT-TFs. (lungdiseasesjournal.com)
- Like FOXF1 , these genes also provide instructions for making transcription factors that regulate development of various body systems before birth. (medlineplus.gov)
Fibroblast growth1
- Mutations in what are called fibroblast growth factor receptors (FGR1, 2, and 3) and the transcription factor TWIST are responsible for several types of craniosynostosis. (yourdictionary.com)
Epidermal1
- A reduction in cell surface expression of adhesion molecules such as integrin β1, major histocompatibility complex class I, and the epidermal growth factor receptor was observed with the loss of cell adhesion. (brainstorming.com)
Differentiation3
- However, once cells are committed to a specific fate, it is critical to restrict the activity of such factors to enable differentiation. (bvsalud.org)
- Altogether, our data reveal that Ubx is a critical player in mediating the silencing of Twist, which is crucial for coordinated muscle differentiation. (bvsalud.org)
- Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. (wikipedia.org)
Overexpression2
- Mesoderm -specific Ubx loss-of-function experiments using CRISPR -Cas9 and overexpression studies demonstrate that Ubx majorly impacts twist transcription. (bvsalud.org)
- In addition, TWIST overexpression can reverse the inhibition of lncRNA SNHG3 knockdown or miR-326 overexpression on cell proliferation, migration, and invasion. (hindawi.com)
Cellular1
- Specifically the effects of various NLS substitutions in TWIST on cellular localization was assayed by immunoflourescence assay. (uni-marburg.de)
Promoter1
- Over-expression of Twist or methylation of its promoter is common in metastatic carcinomas. (wikipedia.org)
Proliferation1
- Direct invasion by carcinoma cells of the stromal compartment involves active proteolysis effected principally by matrix metalloproteinases (MMPs), while degrading the BM and other ECM that lie in the path of invading tumor cells, MMP-expressing cells also liberate growth factors that are sequestered there, thereby fostering cancer cell proliferation. (medscape.com)
Regulation2
- Gene transcription and Regulation 3. (kmu.edu.tw)
- Hypoxia leads to the up-regulation of hypoxia-induced transcription factors (HIF)-a and HIF-2a, which are the master regulators of proangiogenic signals, mainly the vascular endothelial cell growth factors (VEGFs). (medscape.com)
Motifs1
- If anyone knows of somewhere I could download a whole bunch on known binding sequences for these factors it would be very easy to calculate the motifs and matrices. (biostars.org)
Inhibition1
- PYK2 depletion decreased AXL transcription through feedback loops mediated by FRA1 and TAZ, whereas PKCα inhibition induced redistribution of AXL to endosomal/lysosomal compartment and enhanced its degradation. (life-science-alliance.org)
Tumor cells1
- The treatment of HCC depends on the identification of oncogenic factors that lead tumor cells to develop resistance to therapy. (drtaheriazam.com)
EGFR2
- Análisis genómico funcional de la resistencia a las terapias anti-EGFR asociada al fenotipo mesenquimal en el cáncer pulmonar. (uv.es)
- An increase in transforming growth factor α (TGFα) and EGFR in rats with bleomycin-induced lung injury 16 has been observed. (lungdiseasesjournal.com)
Endothelial2
- During EndMT, ECs lose their ability to express endothelial markers, such as vascular endothelial cadherin (VE-cadherin), platelet endothelial cell adhesion molecule (PECAM-1, also known as CD31) and von Willebrand Factor (vWF). (bmbreports.org)
- The development of the tumor vasculature is dependent on the homeostatic balance between a variety of proangiogenic and antiangiogenic (vascular endothelial growth factor and thrombospondin, respectively), inflammatory, and coagulation factors. (medscape.com)
Closely1
- Two factors concur to determine specific Rho GTPase function: tissue specificity of GTPase effectors and distinct intracellular localizations of closely related Rho GTPases, due to different lipid modifications [ 1 ]. (biomedcentral.com)
Cancer7
- Hence targeting Twist has a great promise as a cancer therapeutic. (wikipedia.org)
- Twist also protects cancer cells from apoptotic cell death. (wikipedia.org)
- In addition, Twist is responsible for the maintenance of cancer stem cells and the development of chemotherapy resistance. (wikipedia.org)
- Recently, targeting Twist has gained interest as a target for cancer therapeutics. (wikipedia.org)
- The key factor of cancer associated morbidity and mortality is principally attributable to the development of metastases. (intechopen.com)
- Tumor necrosis factor-α (TNF-α) has been considered stimulated the EMT in several kinds of cancer cells which is a function that contrasts with its more established role in inducing apoptosis [ 7 , 12 , 13 ]. (biomedcentral.com)
- The current review focuses on the role of SOX2 transcription factor in cancer. (drtaheriazam.com)
Diagnostic1
- In conclusion, lncRNA SNHG3 may promote GC progression through the miR-326/TWIST axis, which may provide a new diagnostic and prognostic biomarker for GC. (hindawi.com)
Nuclear4
- First, I focused on functional characterization of the NLS1 and NLS2 domains as potential nuclear localization signals in TWIST. (uni-marburg.de)
- In particular, TWIST NLS1 altered at amino acid position K38R was found to be retained in the cytoplasm of transiently transfected U2-OS cells, suggesting that NLS1 is functional and essential for the nuclear transport of TWIST. (uni-marburg.de)
- Additionally, to understand the role of the TWIST NLS2 in nuclear localization, amino acid at positions 73, 76 and 77 were substituted in this motif. (uni-marburg.de)
- These results demonstrated that substitution at NLS2 position 76 does not play an essential role in the nuclear localization of TWIST, in contrast to the K73R and K77R that inhibit nuclear accumulation. (uni-marburg.de)
Genetic3
- It may be caused by many risk factors such as social environment, lifestyle especially eating habits, lack of physical activity, genetic factors etc. [ 2 , 3 ]. (biomedcentral.com)
- IPF is thought to be a result of a combination of genetic and environmental factors with repetitive micro-injuries to alveolar epithelial cells playing a central role. (lungdiseasesjournal.com)
- Although the cause of IPF is unknown, interacting genetic and environmental factors are thought to play a role in its development 2 . (lungdiseasesjournal.com)
Time3
- Over time, transcription of key factors expands across the rest of the placode and cells switch their behavior from predominantly intercalating to predominantly apically constricting as their position approaches the invagination pit. (elifesciences.org)
- Appalled or not, though, we have no more time to twist our tales. (cdc.gov)
- At 27C, the residence time is estimated to 0.2 ns, a factor of 40 shorter than the tumbling time of the duplex. (lu.se)
Interact1
- Twist transcription factor has been shown to interact with EP300, TCF3 and PCAF. (wikipedia.org)
Risk factors6
- Other recognized risk factors include smoking, the intake of pickled vegetables in the diet, and the clinical management of obesity [ 2 ].The treatment of GC mainly includes surgery, chemotherapy, radiotherapy, and targeted therapy [ 3 ]. (hindawi.com)
- What are the risk factors, job tasks/exposures, and the short- and long-term effects of work-related injuries sustained by truck drivers? (cdc.gov)
- OBJECTIVE: To estimate the prevalence of and risk factors for cardiovascular autonomic neuropathy (CAN) in adolescents and young adults with type 1 and type 2 diabetes enrolled in the SEARCH for Diabetes in Youth Study. (cdc.gov)
- Cross-sectional and longitudinal risk factors were assessed at baseline and follow-up visits. (cdc.gov)
- Area under the curve (AUC) was used to assess the longitudinal glycemic exposure and cardiovascular risk factors. (cdc.gov)
- Suboptimal glycemic control and elevated triglycerides were the modifiable risk factors associated with CAN. (cdc.gov)
Cell2
- The expressions of SNHG3, miR-326, and TWIST in GC9811-P GC cell lines were detected by RT-qPCR. (hindawi.com)
- The results showed that lncRNA SNHG3 and TWIST were highly expressed in GC cell lines, while miR-326 was expressed to a low degree. (hindawi.com)