Tumor tissue tumor cells cultured. Medical search. Frequent questions

Immunostaining revealed that major melanoma-associated antigens, such as S100 protein, HMB-45, melanotransferrin, chondroitin sulphate proteoglycan, and the gangliosides GD2 and GD3, were consistently expressed by the tumour tissue, severe combined immunodeficiency (SCID) mouse xenograft and derived cell lines. (tmu.edu.tw)
The availability of the soft tissue melanoma cell line, the SCID mouse xenograft tumour system as well as autologous TILs described herein would provide useful materials for identifying T-cell-defined antigens as well as a model system for devising individualised cancer biotherapeutic strategies. (tmu.edu.tw)
In mouse and rat xenograft tumor models, TAK-285 showed antitumor activity against cancers that expressed HER2 or EGFR. (jcancer.org)
Solicited models include patient-derived cell lines, patient-derived xenograft (PDX) mouse models, and 3D human tissue model culture systems established from racially/ethnically diverse patient populations. (sbir.gov)
The reduction in tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable to those produced by 5-FU. (spandidos-publications.com)
Both mice model with xenograft prostate tumor model and canine model were used. (hindawi.com)
In addition, NV651 decreased tumour growth in xenograft-mouse model. (lu.se)

MST-1 cells grown in vitro were heterogeneous in morphology, ranging from floating round cells, loosely attached round/oval or elongated cells with prominent pseudopod-like processes, to well-attached spindle and elongated dendritic cells without obvious pseudopods. (tmu.edu.tw)
Our results suggest that single cell-derived subclones from a patient can produce phenotypically heterogeneous self-renewing progenies in both in vitro and in vivo settings. (nature.com)
Regardless of the nomenclatures used, the CSCs (as the cells are commonly termed) that are isolated directly from patients' tumors are considered to serve as valuable tools that can enhance our understanding of tumorigenesis, therapeutic resistances and the functional heterogeneity of cancers in vitro and in vivo 5 . (nature.com)
Not surprisingly, the regulation of this vital safeguard has been studied in great detail for many years but mainly in tissue culture, or in vitro, models. (news-medical.net)
Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
Bioinks can be 3D bioprinted into functional tissue constructs for drug screening, disease modeling, and in vitro transplantation. (sigmaaldrich.com)
CRCs have marked benefits over traditional immortalized cell lines as they are generated using special in vitro conditions that permit cells to pharmacologically bypass replicative senescence without any detectable cell crisis. (sbir.gov)
On the other hand, three-dimensional cultures have recently been attracting attention as in vitro models that more closely resemble the in vivo physiological environment. (medsci.org)
A commonly used in vitro experimental method in cancer research is two-dimensional (2D)-culture using dishes or flasks. (medsci.org)
Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
Direct protein quantification was applied successfully to study in vitro induction in sandwich cultured primary hepatocytes in a 24-well format using the prototypical inducers rifampicin, omeprazole, and phenobarbital. (aspetjournals.org)
Common culture formats used for in vitro enzyme induction studies comprise conventional and sandwich cultures, the former referring to plated hepatocytes maintained on rigid substratum (e.g., collagen), whereas in the latter cells are "sandwiched" by an additional layer of either collagen or a composite of extracellular matrix proteins, such as Matrigel ( LeCluyse, 2001 ). (aspetjournals.org)
Therefore, selection of the appropriate culture format and conditions for a specific in vitro study is crucial with regard to the in vivo predictability of the data generated. (aspetjournals.org)
Short hairpin RNA knockdown of netrin-1 and its receptor UNC5B in EPCAM+ tumour cells inhibited EMT in vitro in the absence of stromal cells and regulated a common gene signature that promotes tumour epithelial state and restricts EMT. (bvsalud.org)
Células cultivadas in vitro procedentes de tejido neoplásico que se utilizan en el estudio de las capacidades metabólicas y proliferativas de las células tumorales, en el pronóstico de las respuestas clínicas a la quimioterapia, en la detección de nuevos agentes antitumorales y en las investigaciones biológicas básicas. (bvsalud.org)
Cells grown in vitro from neoplastic tissue. (bvsalud.org)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
NV556 treatment in an in vitro 3D human liver Extracellular Matrix (ECM) cultured with a TGFβ1-activated human hepatic stellate cell line led to decreased collagen production. (lu.se)

Although enhanced thymosin β4 (TMSB4X/Tβ4) expression is associated with tumor progression and metastasis, its tumor-promoting functions remain largely unknown. (aacrjournals.org)
Here, it is demonstrated that TGFβ facilitates Tβ4 expression and leads to the activation of myocardin-related transcription factors (MRTF), which are coactivators of serum response factor (SRF) and regulate the expression of genes critical for the epithelial-mesenchymal transition (EMT) and tumor metastasis. (aacrjournals.org)
In conclusion, the TGFβ/Tβ4/MRTF/SRF pathway is critical for metastasis and tumor progression. (aacrjournals.org)
TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
[ 115 ] Moreover, CK19 releasing cells were found to be detectable in up to 65 and 70% of CRC and breast cancer patients, respectively, and correlated with presence of metastasis and poor survival in breast cancer. (medscape.com)
Angiogenesis is essential in the process of carcinogenesis to facilitate tumor progression and metastasis. (spandidos-publications.com)
Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
Traditional clinicopathological parameters, such as tumor size, vascular infiltration and tumor node metastasis stage, do not aid in prediction of individual outcomes or determining risk stratification ( 2 ). (spandidos-publications.com)
In addition, it is further related with occurrence and metastasis of malignant tumors. (medscimonit.com)
Functional analysis determined that DOCK10 , ITGA11 , DAB2 , PDFGRA , VASN , PPAP2B , and LPAR1 are highly expressed in trailblazer cells and required to initiate collective invasion, with DOCK10 essential for metastasis. (jci.org)
Together, our results indicate that spontaneous conversion of the epigenetic state in a subpopulation of cells can promote a transition from in situ to invasive growth through induction of a cooperative form of collective invasion and suggest that therapeutic inhibition of trailblazer cell invasion may help prevent metastasis. (jci.org)
Epithelial-to-mesenchymal transition (EMT) regulates tumour initiation, progression, metastasis and resistance to anti-cancer therapy1-7. (bvsalud.org)

Cytogenetic analysis of Hematology-Oncology specimens for leukemia and solid tumors is available. (wakehealth.edu)
Perspectives of tumor-infiltrating lymphocyte treatment in solid tumors. (msdmanuals.com)

Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)
Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
However, the cellular environment under 2D culture is very different and often a poor reflection of the complex tumor microenvironment (TME) in vivo [ 1 ]. (medsci.org)
We are interested in whether age-dependent accumulation of senescent cells leads to alterations in tissue microenvironment that is favorable for oncogenesis. (umassmed.edu)

In order to distinguish between KA and SCC, further histologic studies can be used, including proliferating-cell nuclear antigen immunostaining. (medscape.com)
[ 7 ] In KAs, cells that stain positive with proliferating-cell nuclear antigen immunostaining are distributed only in the outer edges of the tumor, corresponding to the proliferating squamous epithelial cells. (medscape.com)
In contrast, cells within an SCC that stain positive with proliferating-cell nuclear antigen immunostaining are more diffusely distributed. (medscape.com)

Although these tumors are considered to be non-malignant, they have a high rate of proliferation and can reach large sizes before being diagnosed. (frontiersin.org)
Cancer-preventing strategies of these beneficial bacteria such as binding to carcinogens and degrading them, stimulating anti-cancer enzymes, and preventing the conversion of procarcinogens to carcinogens, production of beneficial compounds that act as signaling molecules affecting the immune system, cell death, and proliferation, and interference with cell signaling pathways are reported [ 16 - 19 ]. (hindawi.com)
Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
Once a tissue sample is received, it is enzymatically digested, directly embedded into a 3D environment, and fed with growth factor and Wnt ligand-rich media that stimulates cancer cell proliferation and organoid formation. (corning.com)
Proliferation of the cancer cells was significantly increased through the steroid sulfatase pathway, which metabolizes androgens, estrone sulfate, and estradiol sulfate as its substrates. (scirp.org)
We evaluated the effects of STS inhibitor and aromatase inhibitors on the proliferation of estrogen-dependent endometrial cancer cells. (scirp.org)
Enhancer of zeste homolog 2 (EZH2) belongs to poly-sulfur protein family and can mediate cell proliferation and differentiation via the modulation of various genes expressions. (medscimonit.com)
This study investigated the effect of EZH2 expression on proliferation and tumorigenesis of brain glioma cells. (medscimonit.com)
Clonal formation assay analyzed the change of cell proliferation potency. (medscimonit.com)
Our data demonstrated that in brain glioma cells, the decrease of EZH2 level could suppress cell proliferation and tumorigenesis potency, and meanwhile inhibit the expressions of oncogenes including c-myc and Akt. (medscimonit.com)
Furthermore, aberrant oncogenic activation, DNA damage or oxidative stress activates senescence, providing a failsafe mechanism that prevents the proliferation of cells at risk of neoplastic transformation. (umassmed.edu)
A) Lymphomas found in Smurf2-deficient mice have the characteristics of diffuse large B-cell lymphoma (DLBCL) and enhanced cell proliferation. (umassmed.edu)
BACKGROUND: In the era of "precision medicine," the availability of high-quality tumor biomarker tests is critical and tumor proliferation evaluated by Ki-67 antibody is one of the most important prognostic factors in breast cancer. (bvsalud.org)
or physiological or as a description of key events and processes starting toxic effects that affect cell proliferation. (cdc.gov)

The overall treatment outcome of malignant brain tumors remains unsatisfactory even though advanced multimodal treatments including surgery, chemotherapy and radiotherapy have been available for decades. (nature.com)
The median survival of patients with glioblastoma multiforme (GBM), the most aggressive malignant brain tumor, is typically less than 2 years 1 . (nature.com)
As with all tumors, whether benign or malignant, identification of the cell or cell layer of origin is of the utmost importance. (medscape.com)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
Brain glioma is a type of common primary intracranial malignant tumor, the prognosis of which is frequently unfavorable. (medscimonit.com)
As with all tumors, whether benign or malignant, the paradigm of comprehension depends on identifying the cell or cell layer of origin. (medscape.com)

Molecular Cell Biology. (wikipedia.org)
These findings define a molecular mechanism underlying a tumor-promoting function of thymosin β4 through activation of MRTF/SRF signaling. (aacrjournals.org)
Molecular characterization of these benign yet rapidly proliferating tumors has been limited to evaluating a few mutations in few genes. (frontiersin.org)
The cellular cascade of molecular signals that instructs cells with fatally damaged DNA to self-destruct pivots on the p53 tumor suppressor gene. (news-medical.net)
It was during this time that I developed a passion for studying cancer stem cell biology and the molecular signaling that promotes glioblastoma (GBM) tumor progression. (labmanager.com)
As the former director of the Starr Foundation Cerebral Organoid Translational Core at Weill Cornell Medicine, I established a novel, ex vivo 3D system to study the interactions and molecular crosstalk between brain tumor cells and a miniature model of the human brain. (labmanager.com)
Through use of a library of close to 70 patient-derived organoids, Tiriac has demonstrated that organoids reflect the molecular details of the tumor from which they were derived. (corning.com)
With the development of next-generation sequencing (NGS), research has focused on comprehensive genomic analysis based on tumor molecular features, such as somatic mutations and copy number variation. (spandidos-publications.com)
Cultured hepatocytes offer the possibility to assess long-term effects, such as enzyme induction (i.e., changes of the expression levels of molecular entities of the hepatocytes). (aspetjournals.org)
Projects in the Brain Tumor Stem Cell Research Lab investigate the effect of ion transporters in brain tumor cell migration and the molecular engines that drive tumor invasion. (mayo.edu)
Dr. Quinones-Hinojosa's lab is studying how the NKCC1 protein maneuvers the cell during migration and using findings to develop new molecular targets for targeted cancer therapy. (mayo.edu)
Canonical mesenchymal markers were not sufficient to distinguish trailblazer cells from non-trailblazer cells, suggesting that defining the molecular underpinnings of the trailblazer phenotype could reveal collective invasion-specific mechanisms. (jci.org)
Adjusting the molecular size, the valency and the pharmacokinetics of drug conjugates are as many leverages to improve their therapeutic window, notably by affecting tumor penetration, renal clearance, and short systemic exposure. (bvsalud.org)
Major emphasis is placed is involved in identifying molecular molecular events and pathways that on discerning events that precede or biomarkers of exposure and cancer underpin carcinogenesis, thereby elu- drive tumour initiation and progression risk. (who.int)
First, MCA studies are of specific cancers and surrogate the Epigenetics Group (EGE) and the aimed at providing critical insights into tissues and to identify signatures of Molecular Mechanisms and Biomarkers mechanisms of carcinogenesis through cancer risk and exposures. (who.int)

To further study the role of tTG in liver disease, we initiated investigations into the effect of a proinflammatory mediator, tumor necrosis factor (TNF)-alpha, on tTG activity in cultured liver cells. (nih.gov)
these cultures are also difficult to maintain long-term due to factors such as hypoxia, necrosis, and loss of key genetic features. (labmanager.com)
Tumour necrosis factor in serum and synovial fluid of patients with active and severe rheumatoid arthritis. (bmj.com)
Fifteen serum samples and 29 synovial fluids of patients with rheumatoid arthritis (RA) were examined for the presence of tumour necrosis factor (TNF). (bmj.com)
Tumour necrosis factor was also found in the synovial fluid of 16 out of 29 patients. (bmj.com)
Tumour necrosis factor was not detected in the serum of normal subjects or in synovial fluid of patients with osteoarthritis. (bmj.com)
tumor necrosis factor receptor superfamily. (wikigenes.org)

This cell line can also be used for further studies aimed at uncovering the histogenesis of this rare cancer. (tmu.edu.tw)
IPA analysis of the 280 significantly differentially expressed genes in the whole blood was suggestive of increased hematopoiesis and cell development, activation of cancer/tumor development pathways, and atopy. (cdc.gov)
Because most cancers have a clonal origin, cancer stem cells (CSCs) must generate phenotypically diverse progenies including mature CSCs that can self-renew indefinitely and differentiated cancer cells that possess limited proliferative potential. (nature.com)
The cancer stem cell (CSC) hypothesis, which was first described in studies on leukemia, has attracted considerable attention in other cancer fields, including the one devoted to brain tumor 4 . (nature.com)
When either the sphere-forming system or the adherent system is used, some of the cancer cells can be propagated but other cells are lost because of cell death/apoptosis or differentiation. (nature.com)
Two retrospective studies from Israel 12 and Japan 13 recently reported that diabetes was negatively correlated to overall survival in patients with non-small cell lung cancer (NSCLC) treated with ICIs. (nature.com)
Breast cancer is one of the most common primary tumors to develop associated brain metastases ( 1 , 2 ), and while recent progress in cancer therapy has improved control of primary breast tumors, methods to effectively treat brain metastases from breast cancer remain inadequate. (jcancer.org)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)
If p53 is inactivated, as it is in over half of all human cancers, checks and balances on cell growth fail to operate, and body cells start to accumulate mutations, which ultimately may lead to cancer. (news-medical.net)
Many chemotherapeutical drugs used to treat cancer exert their biological effects on tumor cells through activation of the p53 pathway. (news-medical.net)
Unless promptly and properly repaired, these DNA breaks can let cell division spiral out of control, ultimately causing cancer. (news-medical.net)
We tested an essential-oil derivative, camphor white oil (CWO), for anti-tumor activity in a mouse model of keratinocyte-derived skin cancer. (biorxiv.org)
Common examples include cancer tumorspheres, which enable the study of oxygen gradients and nutrient access in tumor formation. (sigmaaldrich.com)
[ 115-117 ] In cancer cell line spike-in experiments, this method was capable of detecting one cancer cell per 5 ml blood. (medscape.com)
Also, cathepsin D or MUC1 secreting cells were successfully detected in breast cancer patients but not in healthy controls. (medscape.com)
Amanda Linkous , PhD, research associate professor, Department of Biochemistry and scientific center manager, NCI Center for Systems Biology of Small Cell Lung Cancer at Vanderbilt University , talks to contributing editor Tanuja Koppal, PhD, about new scientific and technological advancements in 3D cell culture. (labmanager.com)
Spheroids, however, are cell aggregates typically composed of cancer cells cultured under scaffold-free, non-adherent conditions. (labmanager.com)
Cancer cell lines: The use of immortalized cell lines in cancer research has been standard practice for decades. (sbir.gov)
Notably, the scientific integrity of cancer cell lines is critical for maintaining high standards in research. (sbir.gov)
3D human tissue model culture systems: While immortalized cell lines have been standard practice in cancer research for decades, adequate modeling of the heterogeneity of human cancer is an unmet need. (sbir.gov)
Researchers in Japan have replicated cancer cells from diseased bladder tissue in dogs, minimizing the use of costly stem cell products. (eurekalert.org)
The synthesized tumor cells allow scientists to diagnose cancer and optimize treatment without putting the patient through tiresome rigors of chemotherapy trial and error. (eurekalert.org)
To study this new cell culture approach, the researchers focused on bladder cancer in dogs, a disease which occurs at a rate similar to humans. (eurekalert.org)
Once the team succeeded in creating organoids from the dogs' diseased cells, they tested their response to three common anti-cancer drugs as well as several antibodies. (eurekalert.org)
We are confident that our method will make a breakthrough in bladder cancer diagnosis and treatment in both animals and humans, as it efficiently reduces the culture time, handling, gel, and media supplement costs, and therefore it can be an important platform for the development of new bladder cancer therapy," Usui said. (eurekalert.org)
In this study, the cytotoxicity effects of L. rhamnosus supernatant and whole-cell culture on a cancer cell line (Kyse30) compared to 5fu were evaluated by the MTT assay. (hindawi.com)
Esophageal cancer is a malignancy in the esophagus tissue spread in developing countries. (hindawi.com)
This cancer is divided into two types: (1) squamous cell carcinoma, which appears in the middle or top of the esophagus and (2) adenocarcinoma, seen in the glandular cells of the esophagus [ 1 ]. (hindawi.com)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
In recent years, we focused on investigating the anti-carcinogenic properties of total Astragalus saponins (AST) in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
The efficacy and specificity of CRAD on prostate cancer cells were examined in cell culture and animal models. (hindawi.com)
Circulating PSA-positive cells are also strongly associated with metastatic prostate cancer [ 8 ]. (hindawi.com)
Tiriac's lab then plates multiple domes, a 3D mix of cancer cells and Matrigel , into 6-well dishes containing this media. (corning.com)
In this study, we aimed to evaluate the possible effects of tumor-stromal interactions on local estrogen biosynthesis in endometrial cancer. (scirp.org)
We isolated stromal cells from endometrial cancer patients to examine local biosynthesis of estrogens and tumor-stromal interactions. (scirp.org)
Next we examined the effects of steroid sulfatase inhibitor and aromatase inhibitor in monoculture of endometrial cancer cell line (Ishikawa) and in a co-culture system involving an Ishikawa cells and stromal cells. (scirp.org)
Estrogen receptor and steroid sulfatase mRNA levels in cancer cells were significantly higher in the co-cultures compared with the monocultures of endometrial cancer cells. (scirp.org)
Researchers in Finland have discovered a drug combination that collaborates with the cancer cells' own MYC oncoprotein, which in large quantities causes self-destruction of the cancer cells. (helsinki.fi)
While MYC programs breast cancer cells to build more macromolecules (anabolic metabolism) it also creates a metabolic vulnerability by making them more sensitive to a type of cell death known as apoptosis. (helsinki.fi)
Klefstrom and his research group found that, because of this vulnerability, cancer cells can be attacked with a "drug cocktail" that includes the diabetes drug metformin and venetoclax, a BCL-2 protein inhibitor that can induce apoptosis in cancer cells. (helsinki.fi)
Metformin and venetoclax, when given together, killed breast tumor cells in culture and blocked tumor growth in breast cancer animal models. (helsinki.fi)
Furthermore, the drugs efficiently killed authentic breast cancer tissue donated by breast cancer patients. (helsinki.fi)
This is a great example of how scientists in academia, leveraging highly specialized tumor models and applying their unique insights, can contribute to the discovery of potential new treatments for people with cancer. (helsinki.fi)
one, the cells were truly epithelial, are the cancer cells, the cells from the breast ducts, and the other, however, with the cells that seemed fusiform, not therefore epithelial, that had to be a type. (webofstories.com)
The purpose of this study was to establish a 3D culture method for oral cancer and to verify its practicality. (medsci.org)
Three-dimensional cultures were performed using several oral cancer cell lines. (medsci.org)
Spheroids made from oral cancer cell lines appeared to have tumor-like characteristics that may reflect their clinical significance. (medsci.org)
In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
Furthermore, it is presumed that most of the characteristics inherent in cancer cells are lost. (medsci.org)
Dr. Quinones-Hinojosa's lab is studying ways to engineer human fat cells to turn them into cancer-fighting Trojan horses and evaluating a gel to locally administer to patients during brain cancer surgery. (mayo.edu)
The Brain Tumor Stem Cell Research Lab at Mayo Clinic engineers human mesenchymal stem cells with nanotechnology to serve as Trojan horses for the treatment of cancer. (mayo.edu)
Dr. Quinones-Hinojosa's lab is evaluating the efficacy of a gel for administering human fat-derived mesenchymal stem cells within the brain cancer resection cavity during surgery. (mayo.edu)
The lab is creating models to better understand cancer migration and progression, to study invasive cell populations, and to uncover novel therapeutic targets to impact tumor progression. (mayo.edu)
Breast cancer cell lines contain subpopulations of invasive trailblazer and noninvasive opportunist cells. (jci.org)
A ) Representative images of breast cancer spheroids in organotypic culture stained as indicated ( n = 3). (jci.org)
Tumorigenesis is a multi-step process in which a normal cell acquires changes in a number of critical cancer causing genes. (umassmed.edu)
Consistent with this notion, we have found that Smurf2-deficient mouse embryonic fibroblasts exhibit delayed senescence entry and enhanced potential to become immortalized in culture, while Smurf2-deficient mice show increased susceptibility to various types of cancer, including B-cell lymphoma, hepatocellular carcinoma, adenocarcinoma in small intestine and soft tissue sarcoma. (umassmed.edu)
Pharmacological inhibition of netrin-1 by administration of NP137, a netrin-1-blocking monoclonal antibody currently used in clinical trials in human cancer (ClinicalTrials.gov identifier NCT02977195 ), decreased the proportion of EMT tumour cells in skin SCC, decreased the number of metastases and increased the sensitivity of tumour cells to chemotherapy. (bvsalud.org)
By contrast, administration of NP137 prevented the progression of cancer cells towards a late EMT state and sustained tumour epithelial states. (bvsalud.org)
To assess the relevance of these findings to human cancers, we treated mice transplanted with the A549 human cancer cell line-which undergoes EMT following TGFß1 administration8,9-with NP137. (bvsalud.org)
Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
Therefore CAR T cells and TCR T cells may represent complementary approaches to cancer therapy. (msdmanuals.com)
CAR T cell immunotherapy for human cancer. (msdmanuals.com)
In a prior study, we reported the synergistic cytotoxic effects of gemcitabine and trabectedin on pancreatic cancer cells. (bvsalud.org)
To investigate potential mechanisms underlying this synergistic pharmacodynamic interaction, liquid chromatography-mass spectrometry-based proteomic analysis was performed, and a system pharmacodynamics model (SPD) was developed to capture pancreatic cancer cell responses to gemcitabine and trabectedin, alone and combined, at the proteome level. (bvsalud.org)
In ongoing projects, the candidate may be able to identify and pursue questions pertaining to the chemical characteristics in tissues that are normal or undergoing transformation (cancer). (lu.se)
Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
Based on the consistent expression patterns associated with patient survival outcomes and in tumors vs. normal lung tissues, 10 miRNAs were considered to be putatively tumor suppressive and 4 miRNAs were deemed as oncogenic in lung cancer. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
Targeting the cell cycle has been proposed as a therapeutic strategy in cancer treatment with special interest in the mitotic phase. (lu.se)
arations verified the presence of cancer cells in all samples. (lu.se)

Histologically, the fully formed domelike lesion reveals a mass of proliferating squamous epithelial cells, which created the central core of keratin. (medscape.com)
however, mechanisms through which these bioactive compounds act on epithelial cells have not been identified. (biorxiv.org)
It was concluded that circulating epithelial cells may be present in patients with benign inflammatory colon which were detectable by CTC assays. (medscape.com)
And, observing the cultures of these epithelial cells, I noted that when the cells grew in the cultures, on glass plates, the cells always became more numerous, they started to develop what we call domes, therefore they broke away from the substrate and grew forming this dome. (webofstories.com)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

KA lesions can easily be mistaken for squamous cell carcinoma (SCC). (medscape.com)
SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)
The present study aimed to screen and explore the key circRNA/microRNA (miRNA or miR)/mRNA interactions in head and neck squamous cell carcinoma (HNSCC) using bioinformatics. (spandidos-publications.com)
Head and neck squamous cell carcinoma (HNSCC) is a common malignancy with ~600,000 cases new each year worldwide. (spandidos-publications.com)
For example, circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis ( 9 ). (spandidos-publications.com)
Here we found that netrin-1 is upregulated in a primary mouse model of skin squamous cell carcinoma (SCC) exhibiting spontaneous EMT. (bvsalud.org)
[ 1 ] The clinical and histopathologic features of necrotizing sialometaplasia often simulate those of malignancies such as squamous cell carcinoma or salivary gland malignancy. (medscape.com)
An inadequate biopsy specimen may lead to the misdiagnosis of squamous cell carcinoma or mucoepidermoid carcinoma. (medscape.com)

Cells will be labeled with luciferase or other reporter genes. (stanford.edu)
Tβ4-knockout B16F1 (Tβ4 KO) clones show significantly diminished expression level of tumor-associated genes, which is regulated by the TGFβ/MRTFs pathway. (aacrjournals.org)
MKL1 and MKL2 ), or robust SRF transcriptional coactivators, have been reported to organize actin cytoskeletal rearrangement and regulate cell motility by controlling the expression of dozens of cytoskeletal/adhesion genes ( 4-6 ). (aacrjournals.org)
In cases where multiple other mutations, in genes such as TP53, ATRX, and others, recurrence of the tumor is a possibility. (frontiersin.org)
In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
β-catenin forms a complex with T-cell factor ( TCF ) /lymphoid enhancer factor ( LEF ) transcription factors and cofactors such as histone acetyltransferase p300 (p300), CBP, BCL9, and pygopus, to transcribe Wnt signaling target genes such as Cyclin D1 , C-Myc , and survivin [ 9 , 12 - 15 ]. (hindawi.com)
A new study shows that an enzyme involved in appropriately repressing sets of neuronal genes in non-neuronal cells is also a target for certain monoamine oxidase inhibitors used to treat depression. (sciencedaily.com)
In 2005, professor Ramin Shiekhattar, Ph.D., at The Wistar Institute and his colleagues reported details about an enzyme involved in appropriately repressing sets of neuronal genes in non-neuronal cells. (sciencedaily.com)
FUT6 and CGNL1 were downregulated in HNSCC compared with adjacent healthy tissue and the expression levels of these genes were associated with tumor stage. (spandidos-publications.com)
Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)

Phenotypes collected include multiple system atrophy, progressive supranuclear palsy, multiple sclerosis, and brain tumors. (alzforum.org)

Treatment of human Hep G2 cells with 1 ng/ml TNF-alpha increased [14C]putrescine cross-linking to cellular proteins. (nih.gov)
When higher than normal levels of p53 tumor suppressor exist, there is enough p53 to bind to many regulatory sites in the cell's genome to activate the production of other proteins that will halt cell division if the DNA damage can be repaired. (news-medical.net)
The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
The cultures were exposed to cisplatin and cetuximab and the morphological changes of spheroids over time and the expression changes of target proteins were compared. (medsci.org)
Here, we report the use of a targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for concurrent quantification of multiple cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and transporter proteins in cultured primary human hepatocytes. (aspetjournals.org)
Our recent studies as well as others indicate that senescent cells change their secretory patterns of cytokines, chemokines, growth factors, extracellular matrix proteins and proteases. (umassmed.edu)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)

Here, we identified an epigenetically distinct subpopulation of breast tumor cells that have an enhanced capacity to collectively invade. (jci.org)

Appropriate number of cells will be prepared into single cell suspension in 50-100μL volume (PBS:matrigel=1:1) and injected subcutaneously on the flank or the abdominal area of the mice. (stanford.edu)
Tumor tissue or cells can be implanted into the specific orthotopic sites of the mice. (stanford.edu)
Cells are mixed with matrigel (Corning 356237, non-phenol red, low growth factor) and injected into mice for passaging at 2-5million cells per 50μl SQ. Additional cells can be frozen down for future usage of P1 and P2 passages. (stanford.edu)
Tumor fragments from tumor growing in donor mice will be passaged to recepient mice. (stanford.edu)
We analyzed the effect of PD-1-Ab on tumor growth in streptozotocin-induced diabetic mice (STZ-mice) subcutaneously inoculated with MC38 (a colon carcinoma cell line). (nature.com)
Furthermore, we assessed the expression of chemokines by polymerase chain reaction (PCR) array in tumor-draining lymph nodes (dLNs) of these mice and MC38 cells cultured in different glucose concentrations. (nature.com)
This was accompanied by fewer tumor-infiltrating CD8 + T cells, and STZ-mice had fewer tumor-infiltrating CD11c + dendritic cells (DCs) than normoglycemic mice. (nature.com)
mRNA expression levels of CXCL9, a chemokine recruiting CD8 + T cells, were lower in dLNs of STZ-mice than in normoglycemic mice after PD-1-Ab treatment, and its protein was expressed in DCs. (nature.com)
To investigate the effect of hyperglycemia on anti-tumor function induced by anti-PD-1 antibody treatment, we treated STZ-induced diabetic mice with subcutaneous MC38 colon carcinoma tumors with anti-PD-1 antibody, and examined changes in tumor growth, the number of tumor-infiltrating immune cells, and the expression of chemokines and chemokine receptors. (nature.com)
To induce hyperglycemia, mice were intraperitoneally injected with STZ, which can damage pancreatic β cells resulting in the development of insulin dependent diabetes, a model of type 1 diabetes mellitus (T1DM). (nature.com)
Mice were intraperitoneally injected with STZ or sodium citrate buffer (CB) 7 days before subcutaneous inoculation with MC38 cells in the right flank on day 0 (Fig. 1 A). Then, the mice were treated with anti-PD-1 antibody or normal rat IgG on days 5, 8, and 11. (nature.com)
The effect of anti-PD-1 antibody on MC38 tumor is attenuated in STZ-induced diabetic mice. (nature.com)
Instead of accumulating in mouse cells and halting cell division in the genetically engineered mice, the altered p53 protein performed flawlessly: it was unstable when no DNA damage was present and was stable and fully functional when needed to halt the cycle cell to repair DNA damage or to induce apoptosis. (news-medical.net)
They also reversed the process, generating 3D organoid cells using traditional bioprinting, then planted them into mice. (eurekalert.org)
An in vivo investigation in nude mice was done to assess the anti-tumor activity of L. rhamnosus supernatant and whole-cell culture. (hindawi.com)
Transgenic mice bearing PB promoter fused to simian virus 40 large tumor antigen gene all consistently develop prostate adenocarcinoma [ 13 ]. (hindawi.com)
After the primary tumors were surgically removed, the mice were then treated with a triple combination: metformin, venetoclax and a PD-1-targeted antibody, which is used in immunotherapies to keep killer cells active long-term. (helsinki.fi)
With this combination the survival of mice carrying implanted tumors was extended dramatically in comparison to mice that were treated with only single or double combinations", Klefstrom states. (helsinki.fi)
Owing to their small size and monovalent format, the two Nanofitins displayed a fast and deep tumor penetration in EGFR-positive A431 xenografts in BALB/c nude mice after intravenous administration, yielding to a targeting of respectively 67.9% ± 14.1 and 98.9% ± 0.7 of the tumor cells as demonstrated by IHC. (bvsalud.org)
I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)

3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)
This has led to the development of 3D cell culture technologies and methods that use aggregates of cells, often human primary or differentiated stem cells into tissue or organ-like structures associated with the indications of a disease. (ddw-online.com)
Together with Gen5 Image Prime image analysis software, the platform provides a comprehensive tool for quantitative assays particular to 3D cell culture methods. (ddw-online.com)
The methods can include a variety of mass spectrometry tools, cell culture, tissue sectioning, mathematical mass balance modelling, and isotope geochemistry. (lu.se)

It initiates the process of programmed cell death, or apoptosis, which directs the cell to commit suicide, permanently removing the damaged DNA from the organism. (news-medical.net)
First, they hit breast tumors with apoptosis-inducing drugs metformin and venetoclax to reduce the tumor size and to wake up killer lymphocytes. (helsinki.fi)
Here we describe drug effects on pathways associated with cell cycle, DNA damage response (DDR), and apoptosis, and provide insights into underlying mechanisms. (bvsalud.org)
Drug combination effects on protein changes in the cell cycle- and apoptosis pathways contribute to the synergistic effects observed between gemcitabine and trabectedin. (bvsalud.org)

Administration of supernatant and whole-cell culture of L. rhamnosus expressively reduced tumor growth compared to the control group. (hindawi.com)

To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor requires quantitative evaluation of a large number of cell nuclei from continuous meaningful structures such as milk-ducts, tumors, etc., located relatively far from the primary tumor. (nih.gov)
The study of two cases of ductal carcinoma in situ in breast tissue supports the biological observation regarding the existence of a preferential intraductal invasion, and therefore a common origin, between the primary tumor and the gene amplification in the cell-nuclei lining the ductal structures in the breast. (nih.gov)
Whole blood gene expression profiling is a promising approach to identification of novel biomarkers for tissues over traditional, invasive biopsy. (cdc.gov)
In murine mammary gland cells (NMuMG), Tβ4 upregulation is required for full induction of a MRTF-regulated EMT gene expression program after TGFβ stimulation. (aacrjournals.org)
In multiple human cancers, Tβ4 levels correlate positively with TGFβ1 and the tumor-associated gene expression levels through processes that respectively depend on TGFβ receptor 1 (TGFBR1) and MRTF expression. (aacrjournals.org)
The GMAB technology platform, exclusively licensed from Yale University, utilizes a proprietary, cell-penetrating antibody to non-covalently bind to and deliver therapeutic levels of multiple types of nucleic acids, including synthetic RNA (ribonucleic acid) messenger RNA, small interfering RNA, deoxyribonucleic acids, antisense oligonucleotides and gene editing molecules. (biospace.com)
Studies completed with labeled RNAs and messenger RNAs expressing a green fluorescent protein reporter gene show tumor specific delivery and functional expression by imaging, with minimal delivery to healthy tissues. (biospace.com)
Cell culture studies show antibody-mediated delivery of a series of retinoic acid-inducible gene I (RIG-I) ligands, including hairpin RNAs and synthetic double-stranded RNA (poly(I:C)), resulting in robust RIG-I stimulation and induction of type-1 interferon signaling. (biospace.com)

Tumor-infiltrating lymphocytes (TILs) may have greater tumoricidal activity than LAK cells. (msdmanuals.com)

Neuronal primary cell cultures are cells collected from the brain of an organism. (wikipedia.org)
One of the primary reasons for restricted drug access to the CNS is the blood-brain barrier (BBB), which limits the exposure to the CNS and, therefore limits the effectiveness of pharmacological agents in neuronal tissue. (jcancer.org)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

however, it remains unknown whether the anti-tumor effects of anti-programmed cell death-1 antibody (PD-1-Ab) treatment are changed in hyperglycemic conditions. (nature.com)
After the incubation period, cells are washed out and the released protein spots are detected by the incubation with a fluorochrome-conjugated antibody and counted. (medscape.com)
This antibody, a modified version of 3E10, forms non-covalent complexes with RNAs and can mediate highly specific delivery broadly into tumors via intravenous injection by targeting the nucleoside transporter ENT2. (biospace.com)
GMAB technology utilizes a novel, cell-penetrating antibody to non-covalently bind to and deliver therapeutic levels of a wide variety of nucleic acid payloads to select cells. (biospace.com)
The Ki-67 index was evaluated by 2 pathologists with MIB-1 antibody as a global tumor index and also in a hotspot. (bvsalud.org)
BACKGROUND: Teclistamab, a B-cell maturation antigen × CD3 bispecific antibody, is approved in patients with relapsed/refractory multiple myeloma (RRMM) who have previously received an immunomodulatory agent, a proteasome inhibitor, and an anti-CD38 antibody. (bvsalud.org)

The establishment and characterisation of paired autologous tumour cell line (MST-1) and tumour-infiltrating lymphocyte (TIL) culture from a tumour mass of a 14-year-old Taiwanese girl with soft tissue melanoma are described. (tmu.edu.tw)
This observation and the expression of the major melanoma-associated antigens were all indicative of the melanocytic origin of MST-1 tumour. (tmu.edu.tw)
Murine skin melanoma cells (B16F0 and B16F1) also show the expression regulation of Tβ4 by Smad and TCF/LEF. (aacrjournals.org)
The assay for TNF was based on the cytotoxic activity of this cytokine for human melanoma cells in tissue culture. (bmj.com)
Most importantly, when using a mouse B16 melanoma tumor model, substantial tumor growth suppression was observed upon intravenous injection of GMAB D31N non-covalently bound to 3pRNA, an 89-nucleotide, single-stranded RNA. (biospace.com)

Pretreatment of spheroids with misonidazole before ADR effectively reduced this resistant population of cells. (researchgate.net)
Since our 3D models required gentle agitation within an incubator in order to remain viable, there was a lot of trial and error to determine the best way to effectively image the tumor and return the organoid to a shaking culture environment, without affecting the viability of the sample itself. (labmanager.com)
They captured diseased cells voided in the dogs' urine and replicated them using a new process that generates bodies mimicing key characteristics of the original tumor cells closely enough that they can effectively be used to diagnose disease and identify possible treatments. (eurekalert.org)
Theoretically, a large tumor burden could be effectively eradicated using a small dose of an oncolytic virus. (hindawi.com)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)

Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)

PDX Mouse Models: PDX models are commonly used in many clinically relevant research applications including characterization of tumor heterogeneity, in vivo therapeutic target validation studies, therapeutic mechanism of action studies, and therapeutic sensitivity and resistance studies. (sbir.gov)

These cultures present several advantages over cell lines, including a better representation of the cellular heterogeneity of tissues, a more faithful transcriptomic and proteomic profile (especially when cultured in 3D) and more realistic functional responses, including drug responses. (wikipedia.org)
Although this heterogeneity is generally discussed in terms of pathological structures, examining the dynamic heterogeneity at the cellular level is fundamental to understanding the origins of the cells, potential therapeutic targets and the source of tumor recurrences 3 . (nature.com)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)

Seahorse Metabolic Analyzers service for rapid and quantitative measurement of cellular bioenergetics: Seahorse XF Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate to interrogate key cellular functions such as mitochondrial respiration and glycolysis. (weizmann.ac.il)
GBM, as its name suggests, is composed of a pathologically heterogeneous mixture of cells that exhibit varying degrees of cellular and nuclear polymorphism 2 . (nature.com)
When we placed dissociated glioma tissues on uncoated culture dishes, some cells formed sphere-like aggregates, whereas other cells grew out and extended cellular processes ( Fig. 1a ). (nature.com)
Since the p53 protein is able to trigger such drastic action as cellular suicide, the cells of the body must ensure that the p53 protein is only activated when damage is sensed and that the protein is quickly degraded when it is not needed. (news-medical.net)
Q: Can you discuss some key technical and experimental challenges in culturing and using 3D cellular models? (labmanager.com)
Organoids are cultured from stem cells within a 3D medium to provide a more physiologically relevant and realistic view of cellular interactions than can be seen with traditional 2D models. (corning.com)
The evolution of these mechanisms has given tissues an intelligence with which to maintain their integrity and their autonomy, ordering and separating cellular contents from the surroundings. (weeksmd.com)
The intra- and extra-cellular events that occur in 3D culture are similar to those in a physiological environment [ 3 - 5 ]. (medsci.org)
Dr. Quinones-Hinojosa's lab has research platforms on novel therapeutics for glioblastoma, cell migration and invasion, cellular therapy, and models of brain and spine cancers, and maintains a human tissue bank. (mayo.edu)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)
The SPD model was subsequently incorporated into our previously-established cell cycle model, forming a comprehensive, multi-scale quantification platform for evaluating drug effects across multiple scales, spanning the proteomic-, cellular-, and subcellular levels. (bvsalud.org)

This is a useful method for cells that lose proliferative capacity after being cultured in plates. (stanford.edu)
Senescence limits proliferative capacity of cells and thus impedes the accumulation of multiple mutations necessary for tumorigenesis. (umassmed.edu)

The team works at the intersection between tumor biology, evolution, chemistry, and geology. (lu.se)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Microarrays were prepared in tumor biology. (lu.se)

In this study, we established 4 subclones from a glioblastoma patient and demonstrated clear evidence that a brain tumor contains heterogeneous subclones that exhibit dissimilar morphologies, self-renewal, proliferative capacities and therapeutic sensitivities. (nature.com)

[ 116 , 118 ] However, a recent report found that CK19 releasing cells could also be detected in patients with benign colon diseases such as diverticulosis and Crohn's disease. (medscape.com)
One of the most common benign epithelial tumors is seborrheic keratosis /senile keratosis, which is a hereditary lesion that appears in persons aged more than 30 years. (medscape.com)
On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)
This presence of melanin provides pigmentation to the benign tumor, and this occurrence has been termed pigmented clear cell acanthoma. (medscape.com)

Interleukin-2 (IL-2) expanded TILs had the predominant CD8 + phenotype and the capacity to lyse cells of the cultured autologous tumour. (tmu.edu.tw)
However, primary cells require adequate substrate and nutrient conditions to thrive and after a certain number of divisions they acquire a senescent phenotype, leading to irreversible cell cycle arrest. (wikipedia.org)
Assay conditions, such as culture format and duration, media supplements etc., were reported previously to alter the phenotype and induction response of cultured hepatocytes ( LeCluyse, 2001 ). (aspetjournals.org)

For example, TGFβ induces the phenotypic transition of various types of cells into mesenchymal, myofibroblast, and smooth muscle-like cells, accompanied by dramatic cytoskeletal remodeling and increased cell motility ( 2, 3 ). (aacrjournals.org)

The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)

Mammary fat pad will be identified and breast carcinoma cells will be injected at the concentration of 1-5X10 6 in 30μL PBS or with 50% matrigel. (stanford.edu)
Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
A number of PSPs have been defined over the years that include, but not limited to, promoters of prostate-specific antigen (PSA), probasin (PB), mouse mammary tumor virus (MMTV LTR), prostate-specific membrane antigen (PSMA), human glandular kallikrein 2 (hK2), and prostatic steroid-binding protein C3. (hindawi.com)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

We used colon carcinoma multicellular spheroids containing hypoxic and quiescent cells in core areas for this purpose. (researchgate.net)

Tumors are generated from human tumor cell lines or tissues, then dissected and digested using an enzyme cocktail of DNase I (Sigma 10104159001), trypsin inhibitor (Sigma T9253-5G), and papain (Worthington LS003126) into a single cell suspension. (stanford.edu)
Hep G2 cells, transiently transfected with a luciferase reporter containing 1.67 kb of the human tTG promoter, showed an increase in reporter activity after addition of TNF-alpha. (nih.gov)
HyperSpectral Karyotyping (SKY) of human and mouse cells with ASI Complete Multicolor Karyotyping & FISH System (HiSKY): ASI's HyperSpectral platform is based on Fourier Transform Spectroscopy. (weizmann.ac.il)
We hold NCI60 human tumor cell library. (weizmann.ac.il)
In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). (wikipedia.org)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)
Patient-derived glioma stem cells (green) invading and proliferating within a miniature replica of the human brain. (labmanager.com)
Scanning electron micrograph of two HeLa cells, an immortal human cell line derived from a cancerous tumour of the cervix. (issuu.com)
Collected from Henrietta Lacks in 1951, it was the first human cell line to survive and grow in the laboratory. (issuu.com)
The Brain Tumor Stem Cell Research Laboratory has created a human tissue bank to preserve tissue and cell cultures for use in present and future experiments. (mayo.edu)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)

Several avenues of investigation are being explored in Dr. Quinones-Hinojosa's Brain Tumor Stem Cell Research Laboratory at Mayo Clinic. (mayo.edu)
Researchers in the Brain Tumor Stem Cell Research Lab are investigating the oncostatic effects of melatonin on glioblastoma to improve treatment and provide better quality of life for patients. (mayo.edu)
The Brain Tumor Stem Cell Research Lab studies genetic controls and mediators of tumor growth, invasion, and migration to understand how tumors move into the healthy brain. (mayo.edu)
This is a tool to help students find the right project for their Bachelor's and Master's thesis at Lund Stem Cell Center. (lu.se)

Statistical analysis of genetic changes within cell nuclei that are far from the primary tumor would help determine whether such changes have occurred prior to tumor invasion. (nih.gov)
In a mouse model featuring xenografts of the subclones, the progression and invasion of tumors and animal survival were also different. (nature.com)
These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)
Tumor cells can engage in a process called collective invasion, in which cohesive groups of cells invade through interstitial tissue. (jci.org)
Analysis of spheroid invasion in an organotypic culture system revealed that these "trailblazer" cells are capable of initiating collective invasion and promote non-trailblazer cell invasion, indicating a commensal relationship among subpopulations within heterogenous tumors. (jci.org)
Arrows indicate patterns of tumor organization that are consistent with collective invasion. (jci.org)

Analysis of lung tissue RNA using Ingenuity Pathway Analysis (IPA) indicated trends in inflammatory response and connective tissue organization markers, with an emphasis on immune cell trafficking, phagocytosis, and adaptive immune responses. (cdc.gov)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)

However, no convincing evidence exists to suggest that only single CSCs are representative of patients' tumors. (nature.com)
Considering that intratumoral estrogen metabolism plays an important role, our co-culture systems provide an environment similar to that of the tumor in living patients in terms of metabolism and synthesis of intratumoral estrogens. (scirp.org)
A total of six pairs of cancerous and adjacent healthy tissue were obtained from patients with HNSCC and genome‑wide transcriptional sequencing was performed. (spandidos-publications.com)
Glioma tumor tissues were collected from 3 patients who received surgery, and the glioma stem cells were then separated, cultured, and identified by flow cytometry. (medscimonit.com)
Fields et al reviewed 500 patients with Merkel cell carcinoma in a single-institution study. (medscape.com)
Pleural fluid analysis biopsy and lavage has increased the diag- was performed for protein concentration, nostic rate, the cause for many patients with lactic dehydrogenase (LDH), cultures, as exudative PE remains unknown or obscure. (who.int)

HeLa cells) or by genetic modification (e.g. (wikipedia.org)
In contrast, immortalized cell lines are known to become homogeneous through the natural selection of specific subpopulations, to undergo genetic drift and to acquire genetic aberrations. (wikipedia.org)

Estradiol and androstenediol concentrations were also significantly higher in the co-cultured cells. (scirp.org)
The effects of culture format (i.e., sandwich culture versus conventional culture) and of dexamethasone (DEX) media concentrations on mRNA, protein, and activity levels were determined for three donors, and protein expression was compared with that in liver. (aspetjournals.org)

We named this cerebral organoid glioma model the "GLICO" model and published our findings in Cell Reports . (labmanager.com)
image: CK5 expression, a basal cell marker, in the 2.5D organoid cells (right) and parental 3D organoids (left). (eurekalert.org)
Interestingly, we were able to grow organoid cells using techniques just shy of the traditional, complete 3D bioprinting process - a sort of 2.5D process - while producing most of the 3D organoid features. (eurekalert.org)

Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)

Organoids, termed "mini-organs" by many, are comprised of organ-specific cell types (from stem cells or progenitor cells) that exhibit lineage-specific differentiation and self-assembly. (labmanager.com)
And these domes had a structure, a completely different appearance from the other cells, therefore it was clearly a phenomenon of differentiation. (webofstories.com)

Or, if the damage is too severe for the breaks to be repaired, critical backup protection, also governed by the p53 tumor suppressor protein, kicks in. (news-medical.net)
Emerging evidence supports that senescence is an important tumor suppressor. (umassmed.edu)

In addition, these rare cutaneous tumors are often difficult to diagnose based solely on the physical examination findings, and the proper diagnosis relies on excisional biopsy with histologic studies. (medscape.com)

Immune checkpoint inhibitors (ICIs), including antibodies against programmed cell death-1 (PD-1) and PD-1 ligand-1 (PD-L1), induce their anti-tumor effects by improving the function of immune cells 11 . (nature.com)
The isolated CTCs are then cultured in tissue culture plates pre-coated with antibodies which capture the secreted protein of interest. (medscape.com)
With antikeratin antibodies, the clear cell acanthomas stain for AE1 and AE3, but did not stain for CAM5.2. (medscape.com)

Netrin-1 inhibition decreased EMT in these transplanted A549 cells. (bvsalud.org)

Cutaneous tumors encompass a vast array of malignancies, ranging from neuroendocrine to lymphoid in origin. (medscape.com)
The diagnosis and treatment of these cutaneous tumors is constantly evolving, and the role of clinicians relies on accurate diagnosis and a thorough understanding of the clinical sequelae. (medscape.com)
The origin of cutaneous tumors can be simplistically divided into three major categories: epidermal, dermal, and adnexal (see image below). (medscape.com)
Primary cutaneous CD30+ anaplastic large cell lymphomas mimicking KAs have been reported. (medscape.com)
Primary cutaneous MAI infection generally occurs in hosts who are immunocompetent after traumatic introduction into the soft tissue. (medscape.com)
The treatment and diagnosis of many cutaneous tumors is continually changing, and the role of clinicians (eg, primary care physicians, dermatologists, plastic surgeons, pathologists) focuses on an accurate diagnosis and a thorough understanding of the clinical sequelae. (medscape.com)

The goal of this study was to look for correlates between RNA expression in lung tissue and in whole blood after MWCNT exposure. (cdc.gov)
The development and validation of appropriate cell and animal-based models to study underrepresented population groups would greatly advance this field of research. (sbir.gov)
These 3D models are used to study how cells interact with each other within an organ, to gain insight into how disease processes disrupt these interactions, as well as to test the impacts of potential treatments. (corning.com)
So, we embarked upon it, we also decided to continue to study the development in a simpler system, and the simpler system was provided by tissue cultures. (webofstories.com)
In this study, the tumor suppression observed was comparable to the tumor suppression that was observed using anti-CTLA-4. (biospace.com)
In this study, we demonstrate the benefits of the small Nanofitin alternative scaffolds (7 kDa) as selective tumor-targeting modules for the generation of drug conjugates, focusing on Nanofitins B10 and D8 directed against the EGFR. (bvsalud.org)
well as cytologic study for tumour cells. (who.int)

Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines. (wikipedia.org)
Primary cells can become immortalized either spontaneously (e.g. (wikipedia.org)
Because of their requirements for viability, primary cell cultures did not become widespread until the 2000s. (wikipedia.org)
When whole or partial tissues are isolated and maintained ex vivo, the procedure is termed primary tissue culture. (wikipedia.org)
C ) Representative H&E-stained primary breast tumors. (jci.org)

In laboratory settings, glioma stem cells (GSCs) can be isolated by adding EGF and FGF to suspension cultures in the absence of serum, which is the so-called sphere-forming method, or by using adherent culture systems in which specific materials are coated on culture dishes 4 , 6 . (nature.com)
Although antilymphocyte serum was used in the treatment of chronic lymphocytic leukemia and in T-cell and B-cell lymphomas, resulting in temporary decreases in lymphocyte counts or lymph node size, newer humoral immunotherapeutic modalities have been developed. (msdmanuals.com)

however, after the treatment they largely vanished and the remaining killer cells expressed PD-1, a marker of immune cell exhaustion. (helsinki.fi)
To help the immune cells better fight the tumor, the researchers developed a new treatment strategy. (helsinki.fi)
We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)

Spheroid culture is often favored for high-throughput compound screening in drug development and toxicology, where spheroids present more biologically-relevant models than 2D cultures. (sigmaaldrich.com)
Spheroid culture can be achieved in diverse environments including low-attachment microplates, bioreactors, and microfluidics culture systems. (sigmaaldrich.com)
Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)
By using a selective disaggregation technique after intact spheroids had been exposed to the drug it was possible to show directly that the inner spheroid cells were most resistant (D0 = 0.25 μ/106 cells). (researchgate.net)
Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)
In recent years, a spheroid model produced by three-dimensional (3D)-culture has attracted attention as a better experimental model that is closer to the physiological environment in vivo [ 2 ]. (medsci.org)

However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)
This process relies on the formation of a blastema, a pool of progenitor cells that is formed in response to amputation. (lu.se)

When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Each cell line formed spheroidal cell aggregates and showed enhancement of cell adhesion molecules over time. (medsci.org)

One of several postulated roles for tissue transglutaminase (tTG) is the stabilization and assembly of extracellular matrix via peptide cross-linking. (nih.gov)
Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)

The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)

Part I described the proteomic workflow and drug effects on the upstream portion of the SPD model related to cell growth and migration, specifically the RTK-, integrin-, GPCR-, and calcium-signaling pathways. (bvsalud.org)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
Several pathways involved in cell cycle and DNA repair were affected upon NV651 treatment. (lu.se)

Gel shift experiments using nuclear extracts from TNF-alpha-treated cells and oligonucleotides containing the tTG nuclear factor (NF)-kappa B motif revealed increased binding, concordant with mRNA data. (nih.gov)
In MC38 cells cultured with 25 mM glucose, mRNA expression of CCL7, a chemokine recruiting DCs, was decreased compared to cells cultured with 5 mM glucose. (nature.com)

Cancers are composed of heterogeneous combinations of cells that exhibit distinct phenotypic characteristics and proliferative potentials. (nature.com)

Together, these studies identify T cell-mediated tumor regression as the mechanism through which a plant-derived essential oil diminishes established tumor burden. (biorxiv.org)
We are generating conditional Smurf2 knockout and transgenic mouse models to dissect the precise mechanism of Smurf2-mediated tumor suppression. (umassmed.edu)

A definitive tissue diagnosis of necrotizing sialometaplasia should exclude the need for radiographic imaging. (medscape.com)

The suppressive effect of PD-1-Ab on tumor growth was attenuated. (nature.com)
These results suggest that the STZ-induced hyperglycemia impairs the effect of PD-1-Ab treatment on MC38 tumor growth, and is accompanied by reduced infiltration of DCs and CD8 + T cells and decreased expression of CCL7 and CXCL9. (nature.com)
If one administers the appropriate growth factor or biological cue, but at the inappropriate time, then there can be wide variation in the resulting cell types that are generated. (labmanager.com)
The vascular endothelial growth factor (VEGF) is a well characterized angiogenic factor known to stimulate angiogenesis within a tumor ( 1 ). (spandidos-publications.com)
When asked how this impacts his work, Tiriac explained: 'We know that, for many cancers, the environment is what stimulates the growth of cells. (corning.com)
The significant growth inhibition by the steroid sulfatase and aromatase inhibitors were also observed in the co-culture system. (scirp.org)

Anatomy19

Organisms9

Diseases101

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Phenomena and Processes46

Disciplines and Occupations1

Information Science5

Health Care8

Xenograft7

Vitro19

Metastasis12

Solid tumors2

Microenvironment4

Proliferating-cell nu3

Proliferation15

Malignant7

Molecular15

Necrosis7

Cancer77

Epithelial cells6

Squamous cell carc8

Genes10

Brain tumors1

Proteins8

Breast tumor cells1

Mice20

Methods4

Apoptosis4

Reduced tumor growth1

Gene8

Lymphocytes1

Neuronal5

Antibody6

Melanoma5

Effectively5

Anticancer drug2

Characterization1

Heterogeneity3

Cellular13

Proliferative capacity2

Biology3

Therapeutic1

Benign4

Phenotype3

Mesenchymal1

Transporters in brain1

Mammary4

Colon carcinoma1

Human11

Cytotoxic1

Stem cell4

Invasion6

Connective tissue2

Patients6

Genetic2

Concentrations2

Organoid3

Scaffold-free1

Differentiation2

Suppressor2

Biopsy1

Antibodies3

Inhibition decreased1

Cutaneous6

Study7

Primary5

Serum2

Immune4

Spheroid6

Progenitor cells2

Aggregates2

Extracellular matrix2

Glioma stem cells1

Pathways3

MRNA2

Characteristics1

Mechanism2

Diagnosis1

Growth6