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  • Tissue
  • Mesenchymal stem cells (MSC) are multipotent stem cells that display the capacity to generate the tissue in which they reside. (bioportfolio.com)
  • Fjelde A: Human tumor cells in tissue culture. (springer.com)
  • Cell-based therapies hold great promise to restore the lost nerve tissue and function for CNS disorders. (jove.com)
  • In our technique we harvest cells from tissue culture to create a cell suspension used to implant directly into the brain. (jove.com)
  • After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (wikipedia.org)
  • In 1980-82 his group first identified (and provided an in vitro assay for) the human mesenchymal stem cell - the precursor of bone, cartilage, adipose tissue and smooth muscle. (wikipedia.org)
  • The myeloma cell line that is used in this process is selected for its ability to grow in tissue culture and for an absence of antibody synthesis. (wikipedia.org)
  • It is a negative regulator of epithelial cell growth in prostate tissue. (wikipedia.org)
  • Test molecules that promote endothelial cell network formation are candidates for tissue regeneration therapies whereas test molecules that inhibit endothelial cell network formation are candidates for anti-cancer therapies. (wikipedia.org)
  • The cells are "plated" in a tissue culture vessel and allowed to grow. (wikipedia.org)
  • pathology
  • Tumor Profiling and Pathology - An integrated histology and molecular biology grading system is used to improve the accuracy of ependymoma diagnosis and prognostication. (wikipedia.org)
  • Acetate
  • CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. (wikipedia.org)
  • As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. (wikipedia.org)
  • Soft Agar
  • This was accompanied by decreased cancer cell viability, reduced colony formation in soft agar, disturbed cell cycle progression, chromatin condensation and mitochondrial fragmentation.Spalax fibroblast conditioned media had no effect on proliferation of noncancerous cells.Obviously, along with adaptation to hypoxia, Spalax has evolved efficient anti-cancer mechanisms yet to be elucidated. (nih.gov)
  • Growth of cancer cells was inhibited by either direct interaction with Spalax fibroblasts or with soluble factors released into culture media and soft agar. (nih.gov)
  • This was accompanied by decreased cancer cell viability, reduced colony formation in soft agar, disturbed cell cycle progression, chromatin condensation and mitochondrial fragmentation. (nih.gov)
  • Spalax fibroblasts suppress colony formation of human breast cancer cells MDA-MB-231 and MCF7 in soft agar. (nih.gov)
  • A) SpFS2240 Cancer cells were grown in soft agar on top of monolayers of mouse, rat and Spalax fibroblasts. (nih.gov)
  • Cancer
  • Mechanism of apoptosis induction in human breast cancer MCF-7 cell by Ruviprase, a small peptide from Daboia russelii russelii venom. (cancerindex.org)
  • Ruviprase, a 4.4 kDa peptide isolated from Daboia russelii russelii venom demonstrated antiproliferative activity against EMT6/AR1, U-87MG, HeLa and MCF-7 cancer cells with an IC50 value of 23.0, 8.8, 5.8 and 4.0 μg ml(-1), respectively. (cancerindex.org)
  • Treatment of cancer stem cells from human colon adenocarcinoma cell line HT-29 with resveratrol and sulindac induced mesenchymal-endothelial transition rate. (bioportfolio.com)
  • In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line. (bioportfolio.com)
  • DNMT (DNA methyltransferase) inhibitors radiosensitize human cancer cells by suppressing DNA repair activity. (nih.gov)
  • The principal objective of this study was to evaluate the effects of DNMT inhibitors on the radiosensitivity of human cancer cell lines, and to elucidate the mechanisms relevant to that process. (nih.gov)
  • A549 (lung cancer) and U373MG (glioblastoma) cells were exposed to radiation with or without six DNMT inhibitors (5-azacytidine, 5-aza-2'-deoxycytidine, zebularine, hydralazine, epigallocatechin gallate, and psammaplin A) for 18 hours prior to radiation, after which cell survival was evaluated via clonogenic assays. (nih.gov)
  • Weisenthal LM, Marsden J: A novel dye exclusion assay for predicting response to cancer chemotherapy. (springer.com)
  • Kondo T: Prediction of response of tumor and host to cancer chemotherapy. (springer.com)
  • We propose that TFP might be capable of inhibiting one or more elements of the DNA DSB repair machinery, thereby increasing the cytotoxicity of bleomycin in lung cancer cells. (diva-portal.org)
  • In vitro experiments showed an extraordinary ability of normal Spalax cultured fibroblasts to restrict malignant behavior in a broad spectrum of human-derived and in newly isolated Spalax 3MCA-induced cancer cell lines. (nih.gov)
  • Cells from another cancer resistant subterranean mammal, the naked mole rat, were also tested for direct effect on cancer cells and, similar to Spalax, demonstrated anti-cancer activity. (nih.gov)
  • No effect on cancer cells was observed using fibroblasts from mouse, rat or Acomys. (nih.gov)
  • This report provides pioneering evidence that Spalax is not only resistant to spontaneous cancer but also to experimentally induced cancer, and shows the unique ability of Spalax normal fibroblasts to inhibit growth and kill cancer cells, but not normal cells, either through direct fibroblast-cancer cell interaction or via soluble factors. (nih.gov)
  • Exploring the molecular mechanisms allowing Spalax to survive in extreme environments and to escape cancer as well as to kill homologous and heterologous cancer cells may hold the key for understanding the molecular nature of host resistance to cancer and identify new anti-cancer strategies for treating humans. (nih.gov)
  • A) MDA-MB-231 cells (5 × 103 cells) cells were suspended in 0.35% agar and added as the cancer cell top layer to base layer either empty (blank) or containing the Spalax or rat fibroblast monolayer. (nih.gov)
  • In vitro activity of aplidine, a new marine-derived anti-cancer compound, on freshly explanted clonogenic human tumour cells and haematopoietic-precursor cells," British Journal of Cancer, vol. 78, No. 6, 739-744, 1998. (patentgenius.com)
  • Dehydrodidemnin B (DDB) a new marine derived anti-cancer agent (MDA) with activity against experimental tumor models" and Biological activity of thiocoraline. (patentgenius.com)
  • Polamine contents of human breast cancer cells treated with the cytotoxic agents chlorpheniramine and dehydrodidemnin B," Cancer Letters, vol. 113, Nos. (patentgenius.com)
  • Tumorsphere culture enriches and expands tumor cells, thus providing important resources for cancer studies. (jove.com)
  • The traditional subcutaneous tumor model is less than ideal for studying colorectal cancer. (jove.com)
  • Orthotopic mouse models of colorectal cancer, which feature cancer cells growing in their natural location, replicate human disease with high fidelity. (jove.com)
  • One technique involves injection of a colorectal cancer cell suspension into the cecal wall. (jove.com)
  • Cancer cells are first grown in culture, harvested when subconfluent and prepared as a single cell suspension. (jove.com)
  • In the Dec. 1, 2004 Cancer Research , his team reviews cases of illness in captured sharks and related fish that had been filed with the Registry of Tumors in Lower Animals in Sterling, Va. The team turned up 42 cases affecting 21 species. (phschool.com)
  • The Collaborative Ependymoma Research Network (CERN) Foundation is a Nonprofit organization composed of scientists and adult and pediatric cancer researchers who work together to develop new treatments for Ependymoma, a type of primary brain or spinal cord tumor that occurs in both children and adults, and improve the outcomes and care of patients. (wikipedia.org)
  • Cancer Cell", September 2011. (wikipedia.org)
  • born January 18, 1944) is the Enid A. Haupt Chair of Cell Biology at the Memorial Sloan-Kettering Cancer Center. (wikipedia.org)
  • He was the first (1970) to prove the existence of cancer stem cells in a study of leukemia. (wikipedia.org)
  • Most recently Dr Moore has been studying the cancer stem cell in lung cancer and ovarian cancer and has developed some novel methods for growing human cancer stem cells in vitro allowing extensive molecular analysis and identification of targets for therapy designed to eliminate this rare cell population that is responsible for aggressive tumor growth and relapse. (wikipedia.org)
  • CSCs are therefore tumorigenic (tumor-forming), perhaps in contrast to other non-tumorigenic cancer cells. (wikipedia.org)
  • Existing cancer treatments have mostly been developed based on animal models, where therapies able to promote tumor shrinkage were deemed effective. (wikipedia.org)
  • The efficacy of cancer treatments is, in the initial stages of testing, often measured by the ablation fraction of tumor mass (fractional kill). (wikipedia.org)
  • Cancer stem cells were first identified by John Dick in acute myeloid leukemia in the late 1990s. (wikipedia.org)
  • This functional heterogeneity among cancer cells has led to the creation of multiple propagation models to account for heterogeneity and differences in tumor-regenerative capacity: the cancer stem cell (CSC) and stochastic model. (wikipedia.org)
  • The cancer stem cell model, also known as the Hierarchical Model proposes that tumors are hierarchically organized (CSCs lying at the apex (Fig. 3). (wikipedia.org)
  • Within the cancer population of the tumors there are cancer stem cells (CSC) that are tumorigenic cells and are biologically distinct from other subpopulations They have two defining features: their long-term ability to self-renew and their capacity to differentiate into progeny that is non-tumorigenic but still contributes to the growth of the tumor. (wikipedia.org)
  • This model suggests that only certain subpopulations of cancer stem cells have the ability to drive the progression of cancer, meaning that there are specific (intrinsic) characteristics that can be identified and then targeted to destroy a tumor long-term without the need to battle the whole tumor. (wikipedia.org)
  • This cell model suggests these mutations could occur to any cell in the body resulting in a cancer. (wikipedia.org)
  • They examined cancer stem cell plasticity in which cancer stem cells can transition between non-cancer stem cells (Non-CSC) and CSC via in situ supporting a more Stochastic model. (wikipedia.org)
  • These isolated B cells are in turn fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and the exaggerated longevity and reproductivity of the myeloma. (wikipedia.org)
  • His research focuses on the development of imaging assays to monitor fundamental cellular/molecular events in living subjects with an emphasis on the detection and management of cancer. (wikipedia.org)
  • Molecular profiling of single circulating tumor cells from lung cancer patients. (wikipedia.org)
  • In addition, variations in CD44 are reported as cell surface markers for some breast and prostate cancer stem cells.In breast cancer research CD44+/CD24- expression is commonly used as a marker for breast CSCs and is used to sort breast cancer cells into a population enriched in cells with stem-like characteristics and has been seen as an indicator of increased survival time in epithelial ovarian cancer patients. (wikipedia.org)
  • All these biological properties are essential to the physiological activities of normal cells, but they are also associated with the pathologic activities of cancer cells. (wikipedia.org)
  • This indicates that CARNs represent a new luminal stem cell population that is an efficient target for oncogenic transformation in prostate cancer In 2010 it was shown that NKX3-1 was controlled by ERG and ESE3 both directly and through induction of EZH2 (Polycomb group pcg). (wikipedia.org)
  • The NKX3-1 protein expression was detected a hormone-responsive, androgen receptor-positive prostate cancer cell line, but was absent from androgen receptor-negative prostate cancer cell lines as well as other cell lines of varied origins. (wikipedia.org)
  • Likewise, test molecules that inhibit tumor cell migration may also have potential as anti-cancer drugs. (wikipedia.org)
  • Matrigel is also commonly used to prepare human tumor xenografts in rodents as part of a cancer drug discovery program. (wikipedia.org)
  • Recently, Showe's research led to the creation of a blood test that could be used to test for lung cancer using a 29-gene assay. (wikipedia.org)
  • The company specializes in developing cell culture media, cell separation products, instruments and other reagents for use in stem cell, immunology, cancer, regenerative medicine and cellular therapy research. (wikipedia.org)
  • It is frequently used in cancer research laboratories to determine the effect of drugs or radiation on proliferating tumor cells as well as for titration of Cell-killing Particles (CKPs) in virus stocks. (wikipedia.org)
  • Any type of cell could be used in an experiment, but since the goal of these experiments in oncological research is the discovery of more effective cancer treatments, human tumor cells are a typical choice. (wikipedia.org)
  • John Edgar Dick FRS FRSC (born 1954) is Canada Research Chair in Stem Cell Biology, Senior Scientist at the Princess Margaret Cancer Centre, University Health Network and Professor in the Department of Molecular Genetics at the University of Toronto in Canada. (wikipedia.org)
  • Dick is credited with first identifying cancer stem cells in certain types of human leukemia. (wikipedia.org)
  • His revolutionary findings highlighted the importance of understanding that not all cancer cells are the same and thus spawned a new direction in cancer research. (wikipedia.org)
  • In 1994, Nature published his paper which described how cancer stem cells grow slowly. (wikipedia.org)
  • Dick explained, "Most kinds of chemotherapy are designed to kill fast-growing cancer cells. (wikipedia.org)
  • To get rid of the cancer, you have to find ways of eliminating the stem cells. (wikipedia.org)
  • In 1997, Dick reported the detection of cancer stem cells at the root of three other forms of leukemia. (wikipedia.org)
  • His model stated that there are different cancer cells and amongst them there is a pecking order in which the abnormal stem cell, is both the key to forming and feeding a cancer. (wikipedia.org)
  • Transcription
  • In rodents, when normal somatotrophs are deprived of the RET ligand, GDNF (Glial Cell Derived Neurotrophic Factor), RET is processed intracellularly to induce overexpression of Pit1 [Transcription factor (gene : POUF1) essential for transcription of Pituitary hormones GH, PRL and TSHb], which in turn leads to p19Arf/p53-dependent apoptosis. (cancerindex.org)
  • CD44 gene transcription is at least in part activated by beta-catenin and Wnt signalling (also linked to tumour development). (wikipedia.org)
  • It acts as a transcription factor that has critical function in prostate development and tumor suppression. (wikipedia.org)
  • The homeodomain-containing transcription factor NKX3A is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. (wikipedia.org)
  • NKX3-1 expression acts as a transcription factor that has been found to play a main role in prostate development and tumor suppression. (wikipedia.org)
  • apoptosis
  • When GDNF deprived, ACRO cells, but not NFPAs, presented marked level of apoptosis that was prevented in the presence of GDNF. (cancerindex.org)
  • Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. (cancerindex.org)
  • The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. (cancerindex.org)
  • Flow-cytometric analysis confirmed the apoptosis induction in MCF-7 cells by Ruviprase where it induced DNA condensation but did not cause mitotic blockage or chromosomal aberration in treated-cells. (cancerindex.org)
  • Immunofluorescence microscopic analysis indicated Ruviprase induced apoptosis in MCF-7 cells through p53 and p21-mediated pathways. (cancerindex.org)
  • In addition, clotrimazole induced apoptosis in OSCC cells, and significantly down-regulated the anti-apoptotic protein Bcl-2 and up-regulated the pro-apoptotic protein Bax. (nih.gov)
  • fraction
  • Pretreatment with psammaplin A increased the sub-G1 fraction of A549 cells, as compared to cells exposed to radiation alone. (nih.gov)
  • Moreover, trifluoperazine also increases the longevity of bleomycin-induced DNA strand breaks in U1810 cells, as shown by both comet assay and fraction of activity released (FAR)-assay. (diva-portal.org)
  • These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation, which will separate the blood into a top layer of plasma, followed by a layer of PBMCs and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. (wikipedia.org)
  • For Cell-killing Particle assays, the surviving fraction of cells is used to approximate the Poisson Distribution of virus particles amongst cells and therefore determine the number of CKPs encountered by each cell. (wikipedia.org)
  • Humans
  • It harbors the largest population of proliferating cells in the adult brain of rodents, monkeys and humans. (wikipedia.org)
  • In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells. (wikipedia.org)
  • Since then, neural progenitor and stem cells have been isolated from various areas of the adult central nervous system, including non-neurogenic areas, such as the spinal cord, and from various species including humans. (wikipedia.org)
  • In-vitro colony forming cell (CFC) assays using normal human bone marrow grown in appropriate semi-solid media such as ColonyGEL have been shown to be useful in predicting the level of clinical myelotoxicity a certain compound might cause if administered to humans. (wikipedia.org)
  • Growth
  • The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. (jove.com)
  • miR-21 Contributes to Human Amniotic Membrane-Derived Mesenchymal Stem Cell Growth and Human Amniotic Membrane-Derived Mesenchymal Stem Cell-Induced Immunoregulation. (bioportfolio.com)
  • Notably, clotrimazole treatment inhibited OSCC tumor growth and cell proliferation in CAL27 xenograft model. (nih.gov)
  • To support that contention, sellers point to studies indicating that something in shark cartilage can inhibit the blood vessel growth that tumors rely on for access to nutrients. (phschool.com)
  • however, only the white cells are capable of growth in culture. (wikipedia.org)
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)
  • In 1983-84 his group succeeded in identifying and purifying a human growth factor, G-CSF, that stimulates white blood cell production (neutrophils). (wikipedia.org)
  • The clonal evolution model, which occurs in both the CSC model and stochastic model, postulates that mutant tumor cells with a growth advantage outproliferate others. (wikipedia.org)
  • Cells in the dominant population have a similar potential for initiating tumor growth (Fig. 4). (wikipedia.org)
  • Cell fusion is a necessary event in the maturation of cells so that they maintain their specific functions throughout growth. (wikipedia.org)
  • Survival
  • In the present study, we investigated the effect of trifluoperazine on (a) survival of bleomycin-treated human non-small cell lung carcinoma U1810 cells, (b) induction and repair of bleomycin-induced DNA strand breaks, and (c) nonhomologous end-joining (NHEJ), the major DNA double-strand break (DSB) repair pathway in mammalian cells. (diva-portal.org)
  • By using a clonogenic survival assay, we show here that concomitant administration of trifluoperazine at a subtoxic concentration enhances the cytotoxicity of bleomycin. (diva-portal.org)
  • A graphical representation of survival versus drug concentration or dose of ionizing radiation is called a cell survival curve. (wikipedia.org)
  • spontaneous
  • In 50 years of Spalax research, spontaneous tumors have never been recorded among thousands of individuals. (nih.gov)
  • This condition can rapidly lead to life-threatening infection, as the body cannot produce leukocytes in response to invading bacteria and viruses, as well as leading to anaemia due to a lack of red blood cells and spontaneous severe bleeding due to deficiency of platelets. (wikipedia.org)
  • differentiate
  • Essentially this theory proposes that all cells have the ability to be tumorigenic making all tumor cells equipotent with the ability to self-renew or differentiate, leading to tumor heterogeneity while others can differentiate into non-CSCs The cell's potential can be influenced by unpredicted genetic or epigenetic factors, resulting in phenotypically diverse cells in both the tumorigenic and non-tumorigenic cells that compose the tumor. (wikipedia.org)
  • Stem cells are characterized by their capacity to differentiate into multiple cell types. (wikipedia.org)
  • rather they have the capability to differentiate into any cell type. (wikipedia.org)
  • These neurospheres can differentiate to form the specified neurons, glial cells, and oligodendrocytes. (wikipedia.org)
  • mesenchymal stem
  • Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are considered a new and favorable source of stem cells for cell replacement-based therapy. (bioportfolio.com)
  • The purpose of this study is to determine the safety and efficacy of intrathecal treatment delivered to the cerebrospinal fluid (CSF) of mesenchymal stem cells in ALS patients every 3 mont. (bioportfolio.com)
  • Primary outcome measure: Evaluation of viability, security and tolerance of the adipose-derived mesenchymal stem cells implant (ASCs) in fistulizing Crohn's disease patients, collecting t. (bioportfolio.com)
  • This study will evaluate the safety and efficacy of mesenchymal stem cells in patients with cirrhosis of liver. (bioportfolio.com)
  • Proteins
  • The chief components of Matrigel are structural proteins such as laminin, nidogen, collagen and heparan sulfate proteoglycans which present cultured cells with the adhesive peptide sequences that they would encounter in their natural environment. (wikipedia.org)
  • Because FOXO proteins regulate neural stem cell homeostasis, FOXO proteins have been used to protect neural stem cells by inhibiting Wnt signaling. (wikipedia.org)
  • mammalian
  • Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO2 for mammalian cells) in a cell incubator. (wikipedia.org)
  • CD44 is expressed in a large number of mammalian cell types. (wikipedia.org)
  • In partnership with Hamilton, STEMCELL produced the ClonaCell EasyPick automated platform for developing mammalian cell lines. (wikipedia.org)
  • This type of cell fusion is widely used for the production of somatic cell hybrids and for nuclear transfer in mammalian cloning. (wikipedia.org)
  • immune
  • B cell immune responses are measured by analyzing antibodies produced through the humoral immune response. (wikipedia.org)
  • EasySep is a column-free immunomagnetic system for isolating immune cells from a variety of species and sample types. (wikipedia.org)
  • Dick is also known for his demonstration of a blood stem cell's ability to replenish the blood system of a mouse, his development of a technique to enable an immune-deficient mouse to carry and produce human blood, and his creation of the world's first mouse with human leukemia. (wikipedia.org)
  • sensitivity
  • Lazarus H, Tegeler W, Mazzone HM, Leroy JG, Boone BA, Foley GE: Determination of sensitivity of individual biopsy specimens to potential inhibitory agents: evaluation of some expiant culture methods as assay systems. (springer.com)
  • endothelial
  • Effects of the compounds on endothelial function were monitored on human vascular endothelium (HUVEC) cell line as well. (cancerindex.org)
  • For example, endothelial cells create intricate spiderweb-like networks on Matrigel coated surfaces but not on plastic surfaces. (wikipedia.org)
  • Hence, Matrigel allows them to observe the process by which endothelial cells construct such networks that are of great research interest. (wikipedia.org)
  • protein
  • The secondary layer (Layer II) provides for a hypocellular gap abutting the former and has been shown to contain a network of functionally correlated Glial Fibrillary Acid Protein (GFAP)-positive astrocytic processes that are linked to junctional complexes, yet lack cell bodies except for the rare neuronal somata. (wikipedia.org)
  • Radioimmunoassays (RIA) and enzyme-linked immunosorbent assays (ELISA), which are direct binding assays for antibodies that use pure preparations of known antigens to create a protein standard. (wikipedia.org)
  • Matrigel is the trade name for a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells produced and marketed by Corning Life Sciences and BD Biosciences. (wikipedia.org)
  • Once miR-155 pri-miRNA is transcribed, this transcript is cleaved by the nuclear microprocessor complex, of which the core components are the RNase III type endonuclease Drosha and the DiGeorge critical region 8 (DGCR8) protein, to produce a 65 nucleotide stem-loop precursor miRNA (pre-mir-155) (see Figure 2). (wikipedia.org)
  • Alternatively, TALEN constructs can be delivered to the cells as mRNAs, which removes the possibility of genomic integration of the TALEN-expressing protein. (wikipedia.org)
  • inhibit
  • The absence of blood vessels in cartilage led to the hypothesis that cartilage cells (also known as chondrocytes ) produce one or more substances that inhibit blood vessel formation. (cancer.gov)
  • Markers
  • By the use of fluorescent antibodies against different lymphocyte cell surface markers it is also possible to follow the proliferation behaviour of different lymphocyte subsets. (wikipedia.org)
  • antigens
  • Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. (wikipedia.org)
  • Serum is isolated from a clotted blood sample, and the circulating antibody that accumulates in the plasma is assayed by methods that include the following: Affinity chromatography, by which antibodies of interest are isolated from solution by the specific binding of antigens anchored to a solid matrix. (wikipedia.org)
  • antibodies
  • A type of white blood cell, the B cell that produces antibodies that bind to the antigen are then harvested from the mouse. (wikipedia.org)
  • The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which consists of genetically identical hybridomas which produce one antibody per culture (monoclonal) rather than mixtures of different antibodies (polyclonal). (wikipedia.org)
  • These cells produce antibodies (a property of B cells) and are immortal (a property of myeloma cells). (wikipedia.org)
  • Since the antibodies in a well are produced by the same B cell, they will be directed towards the same epitope, and are thus monoclonal antibodies. (wikipedia.org)
  • The B cell that produces the desired antibodies can be cloned to produce many identical daughter clones. (wikipedia.org)
  • This often involves the analysis of antiserum samples that contain specific antibodies against the immunizing antigen, and blood lymphocytes from lymphoid organs for examination of T cell-mediated responses. (wikipedia.org)
  • Competitive inhibition assays, which determine the concentration of a particular antigen in an unknown sample based on how successfully it competes with a labelled reference antigen in binding to antibodies bound to a plastic well. (wikipedia.org)
  • It is similar to the ELISA assay in that antibodies bound to plastic wells are used to bind the cytokines secreted by T cells. (wikipedia.org)
  • Select antibodies are used to cross-link targeted cells to the magnetic particles in a standard FACs tube, which is placed in an EasySep magnet. (wikipedia.org)