Tumor serum immunomagnetic. Medical search. Frequent questions

Materials and Methods Isolation tradition and illness of primary human being CD34+ cells and of U937 cells The human being hematopoietic cell collection U937 was cultured in RPMI-1640 (Existence Systems Carlsbad CA USA) comprising 10% fetal bovine serum (FBS) (Existence Technologies) inside a humidified incubator at 37°C and 5% CO2. (acancerjourney.info)
The cells were cultured in RPMI-1640 medium supplemented with 10% (for U266) or 15% (for RPMI 8226 and MM.1S) fetal bovine serum (Hyclone Laboratories, Inc., Logan, UT, USA) and incubated in a humidified atmosphere with 5% CO 2 at 37°C. (spandidos-publications.com)
201T and CALU-3 airway tumor cells were grown to confluency in cell culture plates and Transwell (Costar) filters, respectively, at 37°C in Dulbecco's Modified Eagle's medium with 15% fetal bovine serum. (healthdrugpdf.com)

TIL exhibit tumor-specificity but are obviously impaired in their capacity to eradicate tumor targets in situ within the tumor microenvironment. (biomedcentral.com)

More recently, other potential blood-based markers are emerging as independent parameters for prediction development and outcome in metastatic disease, including circulating tumor microemboli and circulating tumor materials (CTMat). (amegroups.org)
Babatz J, Röllig C, Löbel B, Folprecht G, Haack M, Günther H, Köhne CH, Ehninger G, Schmitz M, Bornhäuser M. Induction of cellular immune responses against carcinoembryonic antigen in patients with metastatic tumors after vaccination with altered peptide ligand-loaded dendritic cells. (nwbio.com)

While conventional animal models have identified an array of potential anti-tumor therapies, mouse models often fail to translate into effective human treatments. (biomedcentral.com)
New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
Despite the profound and sustained anti-tumor responses observed in treating ovarian cancer patients, resistance to PARP inhibitors has emerged in some cases [ 8 ]. (biomedcentral.com)

Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. (mdpi.com)
Melanoma tumour cells exhibit pronounced heterogeneity within a single tumour, amongst concurrent tumours within the same patient and amongst tumours from different patients. (nature.com)

Serum levels of circulating cytokines and chemokines were also assessed. (biomedcentral.com)
2013 ) Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system. (academictree.org)
2011 ) Microchip-based immunomagnetic detection of circulating tumor cells. (academictree.org)
Cristofanilli M, Reuben J, Uhr J. ( 2008 ) Circulating tumor cells in breast cancer: fiction or reality? (academictree.org)
2007 ) Determination of HER2 status using both serum HER2 levels and circulating tumor cells in patients with recurrent breast cancer whose primary tumor was HER2 negative or of unknown HER2 status. (academictree.org)
Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
Circulating tumor cells in the blood stream. (iiarjournals.org)
Circulating tumour cells (CTCs) can be assessed through a minimally invasive blood sample with potential utility as a predictive, prognostic and pharmacodynamic biomarker. (nature.com)
Circulating tumour DNA (ctDNA) from the same patient blood sample, was assessed by ddPCR targeting tumour-specific mutations. (nature.com)
Circulating tumour cells (CTCs), which are responsible for haematogenous spread of tumours, also reflect this heterogeneity. (nature.com)
The presence of high expressing epithelial cell adhesion molecule (EpCAM high ) circulating tumor cells (CTC) enumerated by CellSearch ® in blood of cancer patients is strongly associated with poor prognosis. (oncotarget.com)
The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)

Simultaneous implantation of human PBL, DC and tumor results in a huPBL-NSG model that recapitulates the development of human TIL and allows an assessment of tumor and immune system interaction that cannot be carried out in humans. (biomedcentral.com)
Moreover, since repeated tissue biopsies are invasive, costly and not always feasible, the assessment of tumor characteristics on CTCs, by a peripheral blood sample as a 'liquid biopsy', represents an attractive opportunity. (amegroups.org)

and 0.5 ng/ml tumor necrosis factor α(TNFα) (all cytokines from Peprotech) for dendritic (Langerhans cell LC) differentiation. (acancerjourney.info)
Further functional analyses revealed enhanced degranulation and secretion of pro-inflammatory cytokines such as interferon-γ and tumor necrosis factor-α. (frontiersin.org)

In hematological malignancies, tumor-derived EVs might reprogram the bone marrow environment, suppress antileukemic immunity, mediate drug resistance and interfere with immunotherapies. (nature.com)
CD138 + cells from a single patient with MM were isolated with informed consent from bone marrow (BM) mononuclear cells using positive immunomagnetic column separation (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). (spandidos-publications.com)

Another suggestion is to have intensive postoperative follow-up which includes consultations every 4-6 months, physical examination and evaluation of serum carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and breast cancer-associated antigen 115 D8/DF3 (CA15.3), at each visit. (iiarjournals.org)

Aiming to provide proof-of-concept evidence and methodology for the potential role of serum-derived EVs as biomarkers in leukemic versus healthy samples in this study, we hope to pave the way for future detection of promising biomarkers for imminent disease progression and the identification of potential targets to be used in a therapeutic strategy. (nature.com)
Both MDSC (myeloid-derived suppressor cells) and regulatory T cells are recruited to CRC where they inhibit the anti-tumour immune response, thereby promoting tumour progression. (biomedcentral.com)
In response to transforming growth factor (TGF)-β, interferon (IFN)-β, or interleukin (IL)-35, neutrophils shift from the N1 to the N2 phenotype and support tumor progression [ 4 ]. (biomedcentral.com)

Human cytokines were readily detectible in the serum and exhibited a different profile in animals implanted with PBL alone, tumor alone, and those reconstituted with both. (biomedcentral.com)

Vaccination with tumor lysate-pulsed dendritic cells elicits antigen-specific, cytotoxic T-cells in patients with malignant glioma. (nwbio.com)

CTCs are tumor cells originating from primary sites or metastases that circulate in the patients' bloodstream and are very rarely found in healthy individuals ( 9 , 10 ) ( Figure 1 ). (iiarjournals.org)
Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)

Both proteins were found to be overexpressed e.g. in tumor cells, mediating cell proliferation and migration as well as tumor angiogenesis and neuroinflammation. (immpact-international.org)

Increasing evidence indicated that conventional and targeted therapies could affect tumor-associated immune responses and increase the effectiveness of immunotherapy. (biomedcentral.com)

This humanized platform provides an opportunity to study the two-way interaction that occurs between human immunity and tumor growth, over time, and to manipulate individual components to test hypotheses and potential clinical impact. (biomedcentral.com)
EVs collected from the serum of leukemic samples might correlate with disease stage, drug-/immunological resistance, or might correlate with antileukemic immunity/immune response. (nature.com)

While a basic understanding of the composition and phenotype of TIL has come from the study of human tumors, these studies are limited by patient heterogeneity, a lack of access to corresponding lymphoid tissue, and an inability to directly investigate mechanisms and interactions. (biomedcentral.com)

It has been shown that responses to these agents are related to the mutagenic burden, such as in tumors with BRCA and other homologous recombination (HR) proteins deficiencies [ 10 ]. (biomedcentral.com)

Tumor growth was evaluated over time and the phenotype of recovered splenocytes and TIL characterized by flow cytometry and immunohistochemistry (IHC). (biomedcentral.com)

This epithelial glycoprotein is now recognized as having an important role in tumor biology. (thermofisher.com)

Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)

Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)

As a key transcription factor of the Wnt/β-catenin pathway, LEF1 helps to regulate important genes involved in tumor cell death mechanisms. (oncotarget.com)

VAP-1 also exists in soluble form, probably generated through cleavage of the membrane-bound protein, and can be found in the sera of healthy human subjects. (biomedcentral.com)

CD34+ cells were isolated from umbilical wire blood (CB) from consenting donors using immunomagnetic beads (StemCell Systems Grenoble France). (acancerjourney.info)
Comparison of immunomagnetic beads coated with protein A, protein G, or goat anti-mouse immunoglobulins. (immpact-international.org)
Immunomagnetic beads were prepared using either protein A (PA) or protein G (PG) coupled to magnetic beads for binding antibodies at their Fc region. (immpact-international.org)

Phase I/II study of vaccination with electrofused allogeneic dendritic cells/autologous tumor-derived cells in patients with stage IV renal cell carcinoma. (nwbio.com)

Chimeric Antigen Receptor (CAR) T cells are now standard of care (SOC) for some patients with B cell and plasma cell malignancies and could disrupt the therapeutic landscape of solid tumors. (biomedcentral.com)

Immune reconstitution slowed but could not eliminate tumor growth and this effect required the presence of CD4+ T cell help. (biomedcentral.com)
2009 ) Multiplexed Tumor Marker Profiling at Single-Cell Resolution: Quantifying Breast Cancer Molecular Signatures. (academictree.org)
This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
They were expanded for three times in serum-free X-vivo 15 moderate (Lonza Basel Switzerland) filled with penicillin streptomycin glutamax (Sigma-Aldrich GF 109203X Seelze Germany) and 50 ng/ml of every fms-related tyrosine kinase 3 ligand (FLT3-L) stem cell aspect (SCF) and thrombopoietin (TPO) (Peprotech Hamburg Germany). (acancerjourney.info)
ImmunoCult™-XF T Cell Expansion Medium is a serum-free and xeno-free medium optimized for the in vitro culture and expansion of human T cells isolated from peripheral blood. (stemcell.com)
Immunomagnetic HIV-1 capture analysis was used to determine the cellular origin of cell-free virus particles present in all 30 plasma samples and indices of immune activation were measured using enzyme-linked immunosorbent assays. (lww.com)

The complex interactions that occur between human tumors, tumor infiltrating lymphocytes (TIL) and the systemic immune system are likely to define critical factors in the host response to cancer. (biomedcentral.com)
The work presented in this study carries animal modeling one step further by simultaneously humanizing the immune system of recipient NOD/SCID/IL-2Rγnull (NSG) animals and challenging them with implanted human tumor cells. (biomedcentral.com)
EVs were characterized by transmission electron microscopy and fluorescence nanoparticle tracking analysis, and EV surface protein profiles were analyzed by multiplex bead-based flow cytometry to identify tumor- or immune system-related EVs of AML, ALL, CLL, and healthy samples. (nature.com)
Furthermore, other cells at the interface of the innate and the adaptive immune system (e.g cytokine-induced killer cells (CIK) or invariant natural killer T-cells (iNKT)) are important mediators in antitumor-, autoimmune-, and antimicrobial responses and tumor surveillance. (nature.com)

Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. (thermofisher.com)

Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. (thermofisher.cn)

6 ) emphasized that the inclusion of serum tumor markers is an important factor in the postoperative monitoring of breast cancer patients ( 7 , 8 ). (iiarjournals.org)
EVs were isolated from the serum of acute myeloid leukemia (AML), acute lymphoid leukemia (ALL), chronic lymphoid leukemia (CLL) patients, and healthy volunteers. (nature.com)
sVAP-1 levels were measured in the serum of 31 patients with CRC and 31 age- and sex-matched controls. (biomedcentral.com)

Tumor infiltrating lymphocytes (TIL) are a universal feature of human cancers and may hold an important key to unlocking effective cancer immunotherapy [ 1 - 3 ]. (biomedcentral.com)
Large-scale immunomagnetic selection of CD14+ monocytes to generate dendritic cells for cancer immunotherapy: a phase I study. (nwbio.com)

Special EV surface protein patterns in serum have the potential as noninvasive biomarker candidates to distinguish several disease-related patterns ex vivo or in vivo. (nature.com)

Our goal is to establish a humanized tumor model as a more effective pre-clinical platform for understanding and manipulating TIL. (biomedcentral.com)
Serum creatinine levels were measured by Smith Kline Beecham clinical laboratories. (peptidesprice.com)

The majority of deaths from breast cancer are not due to the primary tumor itself, but are the result of metastasis to other organs in the body ( 2 ). (iiarjournals.org)
The primary cause of tumor-related death in breast cancer (BC) is still represented by distant metastasization. (amegroups.org)
The dissemination of tumor cells from the primary tumor to distant sites through bloodstream cannot be early detected by standard imaging methods. (amegroups.org)

A tumor-bearing huPBL-NSG model was established in which human leukocytes reconstituted secondary lymphoid organs and promoted the accumulation of TIL. (biomedcentral.com)
VAP-1 is downregulated in human CRC and although the molecular basis of this down regulation is not yet known, we suggest it may be part of a mechanism used by the tumour to prevent the recruitment of anti-tumour immune cells. (biomedcentral.com)
In addition to its ability to promote recruitment to the gut via its expression on mucosal vessels, VAP-1 has also been implicated in the recruitment of lymphocytes to several human tumours including head and neck cancer and hepatocellular carcinoma where VAP-1 expressed on tumour endothelium was shown to support lymphocyte binding [ 7 , 8 ]. (biomedcentral.com)

Apparently, the functions of neutrophils described in hematological malignancies are complex and differ from those observed in solid tumors. (biomedcentral.com)

The EdU reagent was diluted to 20?M in serum-free medium, added to the cells and incubated for 4?h. (healthanddietblog.info)

In one study [ 14 ], TIL from implanted tumor were able to migrate to spleen and maintained their characters even after adaptive transfer to another SCID mouse, providing a unique opportunity to investigate the function of TIL and test strategies to eradicate tumor. (biomedcentral.com)

Anatomy14

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