Tumor cells functional clone cells. Medical search. Frequent questions

ITH is a widely recognized feature of solid tumors and poses distinct challenges related to the development of effective therapeutic strategies, including cancer neoantigen vaccines. (nih.gov)
These peptides were found to be shared among specimens of several patients with solid tumors of different origin, such as ovarian, breast, and lung cancer, but were not detected in healthy tissues, giving them the character of TSAs for TCRs. (medigene.com)
Prostate-specific membrane antigen (PSMA) is a type 2 integral membrane glycoprotein that serves as an attractive target for cancer immunotherapy by virtue of its abundant and restricted expression on the surface of prostate carcinomas and the neovasculature of most other solid tumors. (omicsdi.org)
Its diverse TCR clonality, superior tumor-homing ability, and low off-target toxicity endow TIL therapy unique advantages in treating solid tumors compared with other adoptive cellular therapies. (biomedcentral.com)

Tumor necrosis factor (TNF) is a multifunctional cytokine, which induces proliferation or death in a cell type-dependent manner. (elsevierpure.com)
Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
At a fundamental level, cancer is caused by an accumulation of genetic changes that result in unregulated cell growth and proliferation. (medscape.com)
Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)
Nowadays, the molecular functions of this protein have been involved in a variety of cellular functions, such as enhancing proliferation and blocking cell differentiation, enhanced lymphocyte adhesion, and cell morphogenesis. (bvsalud.org)
Divalent ANC28.1 can induce Antigen Independent T cell proliferation (5). (ancell.com)
2017. Divergent roles of p120-catenin isoforms linked to altered cell viability, proliferation, and invasiveness in carcinogen-induced rat skin tumors. . (oregonstate.edu)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
We previously identified Macrophage Migration Inhibitory Factor (MIF) as a novel factor that can support the proliferation and/or survival of NSPCs based on in vitro functional cloning strategy and revealed that MIF can support the proliferation of human brain tumor-initiating cells (BTICs). (elsevierpure.com)
Using gene manipulation (over or downregulation of TPT1) techniques including CRISPR/Cas9-mediated heterozygous gene disruption showed that TPT1 contributed to the regulation of cell proliferation/survival in mouse NSPCs, human embryonic stem cell (hESC) derived-NSPCs, human-induced pluripotent stem cells (hiPSCs) derived-NSPCs and BTICs. (elsevierpure.com)
instead, we found that PDGF-DD stimulated bulk tumor cell proliferation by induction of paracrine mitogenic signaling between heterogeneous malignant cell clones, some of which expressed PDGFRβ. (lu.se)
Fig. 4and encodes a glutamate receptor involved JNJ-26481585 in growth proliferation and survival of glioma and melanoma cells (23). (molecularcircuit.com)
It first binds with high affinity to specific cell-surface receptors and then induces their dimerization, which is essential for activating the tyrosine kinase in the receptor cytoplasmic domain, initiating a signal transduction that results in DNA synthesis and cell proliferation. (embl.de)
Finally, EPB41L4A-AS2 inhibited breast cancer cell proliferation, migration and invasion and induced cell apoptosis in vitro. (cancerindex.org)

Although epigenetically silenced, endogenous retroviral (ERV) genes become activated in tumors and function to ignite immune responses. (frontiersin.org)
These solo-LTRs contain functional sequences like promoters, enhancers, polyadenylation signals and are frequently located near cellular genes. (frontiersin.org)
Transcriptomics analyses showed pathways related to TCR signaling, cytotoxicity and oxidative phosphorylation were significantly enriched in Tet lo found in both regressing and progressing tumors compared with Tet hi , whereas genes related to DNA damage, apoptosis and autophagy were downregulated. (bmj.com)
Driver events in pediatric malignancies can occur through loss of function in tumor suppressor genes or gain of function in proto-oncogenes. (medscape.com)
Studies have also identified other contender genes and genomic disruptions in human pancreatic exocrine tumors classified patients into different subclasses based on tumor histotypes and cancer-specific chromosomal rearrangements ( 7 - 9 ). (amegroups.org)
Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. (ejbiotechnology.info)
In addition, these alterations affect 3 principal categories of genes, as follows: proto-oncogenes, tumor suppressor genes, and DNA repair genes. (medscape.com)
This article briefly discusses tumor suppressor genes and then focuses on the role of proto-oncogenes in childhood cancer. (medscape.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)
Recent advances in understanding the molecular events underlying hypohidrotic ectodermal dysplasia (HED) caused by mutations of the genes encoding proteins of the tumor necrosis factor α (TNFα)-related signaling pathway have been presented. (springer.com)
HED embraces a genetically heterogeneous group of diseases and is due to mutations of several genes that encode components of the tumor necrosis factor α (TNFα)-related signaling pathway (Cluzeau et al. (springer.com)
Human CD32 exists in at least six isoforms originating from three different genes (Fc γ RII A, B, and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses. (adipogen.com)
and have been shown to induce vasculature JNJ-26481585 in the central nervous system (26 27 Interestingly the TMZ-sensitive clone shows increased expression of several genes involved JNJ-26481585 in neurotransmitter signaling such as glutamate receptors (did not correlate with TMZ responsiveness suggesting that new biomarkers of drug responsiveness are sorely needed consistent with more recent bulk GBM genomic analyses which highlight the subgroup limitations of this marker (1). (molecularcircuit.com)
Results Primary B cells exogenously expressing Bcl2 , Myc and Ccnd1 showed factor-independent growth ability, enhanced colony-forming capability and aggressive oncogenicity, unlike the cases observed with the expression of any combination of only two of the genes. (haematologica.org)
We identified CCND3 and NRAS as cooperative genes with Bcl2 and Myc through the functional screening. (haematologica.org)
The study of functional synergism among cancer-causing genes is thus becoming one of the central issues in cancer research. (haematologica.org)
These genes are transcriptionally deregulated as the partner genes of IgH translocation, and are thus thought to perform crucial roles in human B-cell lymphomagenesis. (haematologica.org)
2 A synergistic effect resulting from a combination of two genes from BCL2 , MYC and CCND1 has been implicated in human B-cell lymphomagenesis. (haematologica.org)
7 Importantly, it is believed that other hitherto unknown genes also play important roles in lymphomagenesis in addition to the synergistic effects of the aforementioned two oncogenes since human B-cell lymphomas often show a variety of genes subject to alterations and/or deregulated expression. (haematologica.org)
Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)

This study supports ERV-K env as a single prognostic indicator for better survival of RCC, which we propose represents a new tumor antigen. (frontiersin.org)
The homodimer of prostate-specific membrane antigen is a functional target for cancer therapy. (omicsdi.org)
Prostate-specific membrane antigen (PSMA) was one of the first prostate cancer biomarkers successfully cloned. (omicsdi.org)
In this article, we review the relevant research in PSMA on the 20th anniversary of its cloning.A PubMed search using the keywords "prostate-specific membrane antigen" or "glutamate carboxypeptidase II" provided 1019 results. (omicsdi.org)
Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)
Pre- hematopoietic and other cells, but the Duffy sence of one copy of the deleted CCR5 gene also antigen of erythrocytes (DARC) is the only influences the course of disease as the onset of member expressed on cells of erythroid lineage. (cdc.gov)

It is well known that miR-145-5p (the guide strand) functions as a tumor suppressor in several types of cancer. (oncotarget.com)

Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. (cancerindex.org)
DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)
In this respect, not only known proteins but also non-coding genetic regions of cells are studied as sources of peptides that can be presented by HLA molecules at the surface of tumor cells and be recognized by T cell receptors (TCRs). (medigene.com)
We will discuss three key proteins that function as tumor suppressors implicated in the development of pediatric (and some adult) cancers: pRB, p53, and PTEN. (medscape.com)
TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. (thermofisher.com)
Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product. (wikigenes.org)
Not only do these unique cells lack the microtubule-based axoneme associated with the swimming movement of most sperm, but they also are devoid of actin and myosin, the proteins generally considered to be the key components of amoeboid movement. (fsu.edu)
This resulted in a cohort of significantly differentially expressed proteins between the cell lines. (dcu.ie)
The expression patterns for these proteins were validated, and functional cell-based assays were carried out to establish the role of each protein in their respective clone. (dcu.ie)
The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains. (embl.de)
Several complementary DNA clones encoding related HRGs were identified, all of which are similar to proteins in the epidermal growth factor family. (embl.de)
Exosomal markers include microRNAs like miR-21 and miR-141, plus various proteins that belong in functional groups such as tetraspanins (CD9, CD63 and CD81), heat shock proteins (Hsp70, Hsp73 and Hsp90) and membrane transporters (GTPases) ( 4 ). (spandidos-publications.com)
By screening for alteration in levels and functional status of proteins involved in Ad-REIC-induced apoptosis, we found that BiP/GRP78, an ER-residing chaperone protein, was expressed at higher levels consistently among resistant cells. (elsevierpure.com)
The reprogrammed cancer cells thereby lose their ability to become tumors, and instead activate the immune system to respond to mutated proteins in the tumor. (lu.se)

Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
Tumor cells display a variety of antigens which have been defined by antibodies. (bdbiosciences.com)
Antibodies that specifically react with tumor-associated antigens are useful in understanding the biology of particular tumor types. (bdbiosciences.com)
Medigene AG (Medigene, FSE: MDG1, Prime Standard), a clinical stage immuno-oncology company focusing on the development of T cell immunotherapies, today presents the screening and identification of novel immunogenic tumor-specific antigens (TSAs) derived from non-coding regions of the human genome at the American Association for Cancer Research Virtual Annual Meeting (AACR) 2021. (medigene.com)
NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
Here, we discuss effects of the GB tumor microenvironment on NK-cell functionality, summarize early treatment attempts with ex vivo activated NK cells, and describe relevant CAR target antigens validated with CAR-T cells. (frontiersin.org)

DRAM2 was preferentially upregulated in NSCLC tissues and higher expression of DRAM2 in NSCLC correlated with tumor node metastases stage and lymph node metastasis. (cancerindex.org)
Such TSAs can provide new targets that allow improved discrimination between cancer cells and healthy tissues by T cells. (medigene.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Expression levels of miR-145-5p and miR-145-3p were markedly reduced in cancer tissues, and ectopic expression of these miRNAs inhibited cancer cell aggressiveness, suggesting that both miR-145-3p as well as miR-145-5p acted as antitumor miRNAs. (oncotarget.com)
Currently, the most widely-used TIL production method is to isolate infiltrating lymphocytes from tumor tissues and then culture and expand these cells in vitro. (biomedcentral.com)
uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues. (plos.org)
Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
Heregulin transcripts were identified in several normal tissues and cancer cell lines. (embl.de)
Fingerprints from tumor tissue were generated by single arbitrary primers and compared with fingerprints for normal tissue from the same animal, as well as normal and tumor tissues from different animals. (diethylstilbestrol.co.uk)
Among these amplified DNA fragments, 44 loci exhibited either qualitative or quantitative differences between the tumor tissues and normal kidney tissues. (diethylstilbestrol.co.uk)
Seven of 44 mutated loci also were present in the kidney tissues adjacent to tumors (free of macroscopic tumors). (diethylstilbestrol.co.uk)
In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
Tissues and Cells. (lu.se)

Isolation of T cell clones by limiting dilution from tumor-infiltrating lymphocytes (TILs) permitted functional validation regarding neoantigen specificity. (nih.gov)
Tumor-infiltrating lymphocyte (TIL) therapy is a type of adoptive cellular therapy by harvesting infiltrated lymphocytes from tumors, culturing and amplifying them in vitro and then infusing back to treat patients. (biomedcentral.com)
In contrast to other cancers such as adenocarcinomas of the lung or melanoma, primary brain tumors like GB and low grade gliomas (LGG) are known as rather immunologically "cold" tumors, typically with low numbers of tumor-infiltrating lymphocytes (TILs) ( 4 ), and the mere amount of TILs is not associated with patient survival ( 5 ). (frontiersin.org)
T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. (ancell.com)

High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. (frontiersin.org)
The protein encoded by this gene is an integral plasma membrane protein which promotes cell adhesion. (wikipedia.org)
RB1 encodes the protein pRB and was the first tumor suppressor gene to be molecularly defined. (medscape.com)
T]he FunSeq tool assigns a molecular functional impact score to a mutation based on various features," the authors explained, such as "inter-species conservation, gain or break of transcription factor motifs, disruption of known enhancer-gene interactions, and centrality in the gene regulatory or protein-interaction network. (genomeweb.com)
The CD34 protein was identified almost four decades ago as a biomarker for hematopoietic stem cell progenitors. (bvsalud.org)
Although a complete understanding of this transmembrane protein, including its developmental origins, its stem cell connections, and other functions, is yet to be achieved. (bvsalud.org)
In addition, the paper will review different techniques of single-cell genomic expression at the level of DNA, mRNA, protein and post-translational modifications. (ejbiotechnology.info)
Re-expression of the retinoblastoma-binding protein 2-homolog 1 reveals tumor-suppressive functions in highly metastatic melanoma cells. (wikigenes.org)
[ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
Thus, in the present study, we newly identified translationally-controlled tumor protein-1 (TPT1), which is expressed in the ventricular zone of mouse embryonic brain, as a downstream target of MIF signaling in mouse and human NSPCs and human BTICs. (elsevierpure.com)
Clonal protein manifestation of EGFRvIII was examined with Traditional western blots using EGFRvIII-transfected human being fetal mind cells. (molecularcircuit.com)
In addition, we have cloned the MSP gene and expressed functional protein in bacteria. (fsu.edu)
1999. Localized depolymerization of the major sperm protein cytoskeleton correlates with forward movement of the cell body in the amoeboid movement of nematode sperm. (fsu.edu)
MARCKS and Desmoglein-3 were found to reduce cell motility in vitro upon transient protein knockdown using siRNA. (dcu.ie)
Shootin-1, a relatively uncharacterised protein, was also found to reduce cell motility upon protein knockdown. (dcu.ie)
Shootin-1 and Desmoglein-3 expression were affected by co-culture of the clonal subpopulations, highlighting the heterogeneous nature of the original DLKP cell line and the influences this has on protein expression. (dcu.ie)
A 45-kilodalton protein heregulin-alpha (HRG-alpha) that specifically induced phosphorylation of p185erbB2 was purified from the conditioned medium of a human breast tumor cell line. (embl.de)
cDNA cloning (GenBank Accession Number AF304351), expression of the encoded protein, and functional analysis of cDNA transfected NIH3T3 cells indicated that translation elongation factor-1 delta is a proto-oncogene. (cdc.gov)

The hybridoma secreting the 9.2.27 antibody was generated using a human melanoma cell extract as the immunogen. (bdbiosciences.com)

This immunotherapy approach attempts to overcome the patient's tolerance towards cancer cells and tumor-induced immunosuppression by activating and modifying the patient's T cells outside the body (ex vivo). (medigene.com)
Functional studies demonstrated that the ZNF513 p.R250W and KIF3C p.R410H variants significantly increased the expression of ZNF513 and KIF3C in vitro and in vivo. (nature.com)
Expression of EBF2 in the PDX1 and E-cadherin-expressing and organoid forming pancreatic epithelial CSCs indeed induced ductal and acinar-like structural differentiation in vivo in tumors resulting in tumor suppression. (amegroups.org)
To this end, Dr. Rosenberg and others developed a "Young TIL" approach that rapidly expands TIL for administration without in vitro selection for tumor reactivity [ 8 ], which markedly improves the timeliness of TIL production as well as its survival and efficacy in vivo (Fig. 1 ). (biomedcentral.com)
Nevertheless, ex vivo activation with cytokines can restore cytolytic activity of NK cells against GB, indicating that NK cells have potential for adoptive immunotherapy of GB if potent cytotoxicity can be maintained in vivo . (frontiersin.org)
B-hADAM9 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. (biocytogen.com)
The 1-A05 clone of B-hADAM9 MC38 cells was used for in vivo experiments. (biocytogen.com)
As shown in panel A, B-hADAM9 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. (biocytogen.com)
We are also interested in how the cell controls MSP polymerization in vivo because the localized formation of filaments along the leading edge of the pseudopod appears to be the key event in locomotion. (fsu.edu)
Design and Methods Growth in culture, colony formation and oncogenicity in vivo were assessed in mouse primary B cells exogenously expressing various combinations of Bcl2 , Myc and Ccnd1 . (haematologica.org)
Functional analysis in a responding patient demonstrated rapid in vivo expansion of neoantigen-specific T cell clones that were reactive to mutant neopeptides found in the tumor. (stanfordhealthcare.org)
Down-regulation of BiP with siRNA sensitized the resistant cells to Ad-REIC in vivo as well as in culture. (elsevierpure.com)

Technological evolution and expanding knowledge of tumor biomarkers have invigorated exploration in prostate cancer therapeutics. (omicsdi.org)
Objectives: To evaluate the role of cancer stem cell biomarkers in diagnosis and prognosis of OSCC patients. (bvsalud.org)
Twenty-two reports were sought for retrieval, however, we could not find the full text of 3 studies and12 studies were excluded because the biomarkers were not associated with cancer stem cells. (bvsalud.org)
CONCLUSIONS: Various stem cell biomarkers have been found to have diagnostic and prognostic role in oral squamous cell carcinoma such as Cyclin D1, VEGF-A, GD15, and CD44. (bvsalud.org)
The profound roles of exosomes in homeostasis, stress and several pathological conditions, in conjunction with their selective and cell‑specific composition/function, allude to their use as promising circulating clinical biomarkers of systemic stress and specific pathologic states, and as biocompatible vehicles of therapeutic cargo. (spandidos-publications.com)
Major emphasis is placed is involved in identifying molecular molecular events and pathways that on discerning events that precede or biomarkers of exposure and cancer underpin carcinogenesis, thereby elu- drive tumour initiation and progression risk. (who.int)

As a result, there is an emergent need to explore previously unmapped mechanisms regulating distinct subpopulations of tumor cells and to understand their contribution to tumor progression and treatment response. (lu.se)
Although the exact functional importance of mutated loci is unknown, this study indicates that these altered loci may participate during tumor progression in the kidney. (diethylstilbestrol.co.uk)

Notably, our platform includes a tracking system for each clone as well as a sorting procedure with 100% specificity validated by downstream single cell reverse-transcription PCR and sequencing of TCR chains. (rsc.org)

Glioblastoma (GB) is the most frequent malignant primary brain tumor in adults, without any curative treatment options available at present. (frontiersin.org)
Functional malignant cell heterogeneity in pancreatic neuroendocrine tumors revealed by targeting of PDGF-DD. (lu.se)
Here, making use of a newly generated Pdgfd knockout mouse, we reveal a functionally important malignant cell heterogeneity modulated by PDGF-DD signaling in pancreatic neuroendocrine tumors (PanNET). (lu.se)
Conclusions Bcl2 , Myc and Ccnd1 or Bcl2 , Myc and CCND3 synergistically transformed mouse primary B cells into aggressive malignant cells. (haematologica.org)
Liver tumors occurred in more than 45 patients, 43 of which were associated with androgen use, often in the context of aplastic anemia or other tumors, and were not usually malignant (although two thirds were histologically hepatomas, and the rest were adenomas). (medscape.com)
Hepatic venous outflow obstruction caused by external compression or invasion of the venous lumen is termed secondary BCS and is seen with malignant tumors or large cysts. (medscape.com)

In contrast, Akt-phosphorylation, β-catenin, and c-Myc are repressed in the cells, presumably inhibiting tumor growth. (amegroups.org)
Building on recent studies suggesting that passenger mutations may "weakly affect tumor cell fitness by promoting or inhibiting tumor growth," the authors used a quantitative sequencing strategy known as FunSeq2 to score the predicted functional effects of somatic mutations present in 2,548 PCAWG tumor samples. (genomeweb.com)

We isolated CSCs from low passage PDAC cell lines derived from human tumors and commercially available cell lines. (amegroups.org)

Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)
Overall, we observed that the molecular functional impact has a multimodal distribution suggesting that the canonical dichotomy of drivers and passengers might not necessarily reflect the complex mutational landscape in cancer genomes," the authors wrote. (genomeweb.com)
One of the key areas in stem cell research is the identification of factors capable of promoting the expansion of Neural Stem Cell/Progenitor Cells (NSPCs) and understanding their molecular mechanisms for future use in clinical settings. (elsevierpure.com)
We forecast that clone-specific practical profiling of GBMs can help determine intense clones new tumor driver systems molecular signatures and restorative vulnerabilities emphasizing the potential of tumor treatment at a clone-specific level. (molecularcircuit.com)
RAPD-PCR provides an alternative molecular approach for studying cancer cytogenetics in stilbene estrogen-induced tumors in humans and experimental models. (diethylstilbestrol.co.uk)
prevention by elucidating the molecular etiology and prevention), using in vitro mechanisms by which genetic and models and state-of-the-art approaches The expected outcome of these studies epigenetic alterations alter critical including (epi)genome-wide screens is the opening up of an opportunity molecular pathways and promote cancer and functional genomics. (who.int)

Moreover, in various tumors of the 4 patients examined, no neoantigen-specific TCR clonotypes were identified despite clonal neoantigen expression. (nih.gov)
[ 5 ] Targeted disruption of TP53 in the mouse leads to the development of various tumors. (medscape.com)

2002. Sequencing of the rat beta-catenin gene (Ctnnb1) and mutational analysis of liver tumors induced by 2-amino-3-methylimidazo[4,5-f]quinoline. . (oregonstate.edu)

Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)
Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
Intracranial cell transplantation included shot of 100 0 cells into immuno-compromised (NSG) mice. (molecularcircuit.com)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
Reprogramming skin cells to blood stem cells could potentially provide an unlimited source of cells for transplantation to patients with blood disorders. (lu.se)
Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)

Findings from past pan-cancer analyses indicate that the average tumor contains roughly five driver mutations, the team noted, while the vast majority of alterations fall into the passenger mutation category. (genomeweb.com)
Our analyses demonstrate that tumor growth was delayed in the absence of signaling by PDGF-DD. (lu.se)
Thus, there is a critical need for single cell technologies to conduct fast and multiplexed functional analyses followed by recovery of the clone of interest. (rsc.org)
Execution Analyses to identify evolutionary first principles that make cancer cells grow, spread, and survive microenvironmental challenges. (lu.se)
These genome-wide analyses identified altered regional mutation profiles in people with cancer that distinguished them from people without cancer and reflected tumor burden during therapy. (cdc.gov)

Identification of a novel transcriptional activator, BSAC, by a functional cloning to inhibit tumor necrosis factor-induced cell death. (elsevierpure.com)
Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. (thermofisher.com)

When used to immunize animals, dimer but not monomer elicited antibodies that efficiently recognized PSMA-expressing tumor cells. (omicsdi.org)

Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain. (ox.ac.uk)

The presence of mutated loci in uninvolved (non-tumor) surrounding tissue adjacent to tumors from stilbene estrogen-treated hamsters suggests that these mutations occurred in the early stages of carcinogenesis. (diethylstilbestrol.co.uk)
Somatic mutations in stilbene estrogen-induced Syrian hamster kidney tumors identified by DNA fingerprinting , Journal of carcinogenesis, NCBI PubMed PMC398418 , 2004 Mar. (diethylstilbestrol.co.uk)

It did not react with fetal melanocytes, neuroblastoma LA-N-1 cells or a variety of carcinoma, lymphoid, and fibroblastoid cell lines. (bdbiosciences.com)
In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. (thermofisher.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)
The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

Cancer stem cells (CSCs) and poorly differentiated progenitor cells in pancreatic ductal adenocarcinoma (PDAC) exacerbate hierarchical tissue organization contributing to cell hyperproliferation and therapy resistance. (amegroups.org)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

Background CD8 + T cells are a highly diverse population of cells with distinct phenotypic functions that can influence immunotherapy outcomes. (bmj.com)
Further insights on the roles of CD8 + specificities and TCR avidity of naturally arising tumor-specific T cells, where both high and low avidity T cells recognizing the same peptide-major histocompatibility complex (pMHC) coexist in the same tumor, are crucial for understanding T cell exhaustion and resistance to PD-1 immunotherapy. (bmj.com)
Conclusions Targeting subdominant T cell responses with lower avidity against pMHC affinity neoepitopes showed potential for improving PD-1 immunotherapy. (bmj.com)
A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
We then outline preclinical approaches that employ CAR-NK cells for GB immunotherapy, and give an overview on the ongoing clinical development of ErbB2 (HER2)-specific CAR-NK cells currently applied in a phase I clinical trial in glioblastoma patients. (frontiersin.org)
Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer immunotherapy with encouraging clinical results. (rsc.org)

The DLKP cell line was established from a lymph node metastasis of a primary lung tumour and is described as a poorly differentiated squamous lung carcinoma cell line. (dcu.ie)

Tumor specimens from 259 NSCLC patients were collected and analyzed. (cancerindex.org)
Our approach is highly translational, integrating assessment of clinical specimens with generation and analysis of patient-derived cell culture and mouse tumor xenograft (PDX) models, performed in close collaboration with clinicians in the MGH Thoracic Oncology group. (massgeneral.org)
Note: Tumor Specimens- PD-L1 expression varies by tumor type so screening is recommended to find positive and negative tumor controls. (abcam.com)
Many tumor specimens have some inflammatory macrophages and mononuclear leukocytes. (abcam.com)
Data from whole transcriptome sequencing of 33 breast specimens in the Harbin Medical University Cancer Center cohort and The Cancer Genome Atlas was applied to identify and validate the landscape of tumor suppressor long noncoding RNAs, which was further validated by The Cancer Genome Atlas pancancer data including 33 cancer types and 12,839 patients. (cancerindex.org)

Functional characterization of high and low avidity GSW11-specific CD8 + T cells was conducted using flow cytometry and bulk RNA-seq. (bmj.com)

Here we show that cell differentiation suppresses tumor formation. (amegroups.org)
Cells were transfected with the early B-cell factor-2 (EBF2) to activate progenitor cell differentiation and gauged the tumorigenic potentials of the derived cell subsets. (amegroups.org)
This study reveals a previously unrecognized function of EBF2 in regulating PDAC progenitor cell differentiation and tumor suppression. (amegroups.org)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. (frontiersin.org)
Human M21 cells were stained with either PE Mouse Anti-Human Chondroitin Sulfate antibody (Cat. (bdbiosciences.com)
Antibody ANC28.1 recognizes CD28 expressed on T cells and also on BHK cells transfected with human CD28. (ancell.com)
The following product was used in this experiment: CD202b (TIE2) Monoclonal Antibody (TEK4), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-5987-82, RRID AB_469188. (thermofisher.com)
Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
Single cell suspensions from wild-type MC38 and B-hADAM9 MC38 cultures were stained with species-specific anti-ADAM9 antibody. (biocytogen.com)

NEW YORK - Results from a new analysis of tumor sequences, led by researchers at Yale University, suggest that passenger mutations may not be as benign as previously believed but may work together to influence tumorigenesis in more subtle ways than cancer driver mutations. (genomeweb.com)
With the help of a functionally informative sequencing method called FunSeq2, the researchers identified non-driver, somatic mutations and their predicted functional impact in 2,548 tumors profiled for the Pan Cancer Analysis of Whole Genomes (PCAWG). (genomeweb.com)
Their findings, documented in a paper in Cell on Thursday , indicated that passenger mutations broadly line up with broader tumor signatures and tumor sub-clone features, potentially adding up to influence cancer features in complex ways. (genomeweb.com)
They also explored potential passenger mutation interactions with transcription factor binding sites and other regulatory features in the genome and looked at the relationship between proposed passenger mutations and tumor features overall - from the apparent mutational processes at play to the mutation patterns in tumor sub-clones. (genomeweb.com)
In collaboration with oncologists in the Mass General Center for Thoracic Cancers, we have identified acquired secondary mutations and other genomic alterations that cause drug resistance in the tumors and blood of patients progressing after initial response to targeted therapies. (massgeneral.org)
The results show the ability of RAPD-PCR to detect and isolate, in a single step, DNA sequences representing genetic alterations in stilbene estrogen-induced cancer cells, including losses of heterozygosity, and homozygous deletion and insertion mutations. (diethylstilbestrol.co.uk)
Through whole-genome sequencing of single molecules of circulating cell-free DNA, we found that tumor-derived mutations in cancer genomes are associated with regions of late replication timing and other chromatin features. (cdc.gov)

In vitro studies have shown DLKP-M to be extremely invasive relative to DLKP-SQ, whereas the latter cell line shows high anoikis resistance relative to DLKP-M. As the cells originate from the same tumour, these varied phenotypic characteristics make DLKP a particularly useful model for the study of cellular heterogeneity in lung cancer. (dcu.ie)
The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)

Scatchard analysis of the binding of recombinant HRG to a breast tumor cell line expressing p185erbB2 showed a single high affinity binding site [dissociation constant (Kd) = 105 +/- 15 picomolar]. (embl.de)

Under a collaboration agreement with IRICoR and the Institute for Research in Immunology and Cancer (IRIC) of the Université de Montréal, Canada, Medigene gained access to 47 potential TSAs presented by one of five common HLA types that were identified as novel peptides eluted from the HLA molecules of tumor cells. (medigene.com)
The focus of this thesis was to develop and characterize several genetically modified oncolytic adenoviruses expressing either OX40L alone or OX40L and CD40L, two co-stimulatory molecules capable of engaging both the innate and adaptive arms of the immune system to fight the tumor. (helsinki.fi)
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
The use of cell lines as "biological minipumps" to chronically deliver antinociceptive molecules into the pain-processing centers of the spinal cord represents a newly developed technique for the treatment of pain [ 3 ]. (hindawi.com)
By using skin cells as the starting cell-type, he managed to identify the three molecules needed to "program" dendritic cells in just nine days. (lu.se)
In the next step, he used the same molecules to reprogram cancer cells into dendritic cells. (lu.se)
In other words, using the molecules as a trojan horse to "force" cancer cells to develop into immune cells. (lu.se)
Our findings are paving the way for the development of a gene therapy for cancer where the three molecules are delivered directly into tumors in patients eliciting a response independently of the tumor type. (lu.se)

Синдроми лізису пухлини та вивільнення цитокінів Adverse effects are common in patients receiving any cancer therapy, particularly cytopenias, gastrointestinal effects, and tumor lysis and cytokine release syndromes. (msdmanuals.com)

The integration of genomics techniques with sophisticated bioinformatic analysis and most importantly clonal functional assays provide a direct starting point as it will identify tumor subpopulations that drive growth and therapeutic resistance. (molecularcircuit.com)
This cell line is comprised of three distinct subpopulations: DLKP-SQ, DLKP-M and DLKP-I, each with well-defined phenotypes. (dcu.ie)
Quantitative label-free LC-MS/MS was used to analyse the DLKP cell line and its subpopulations at a proteomic level. (dcu.ie)

CD34 expression of these stem cells has been exploited for therapeutic purposes in various hematological disorders. (bvsalud.org)
Furthermore, CD34 expression may also be found on a variety of cancer stem cells. (bvsalud.org)
In this paper, we aimed to carry out a systematic analysis of the structure, functions, and relationship with cancer stem cells of CD34 based on the literature overview. (bvsalud.org)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
He has succeeded in converting human skin cells into blood stem cells, which is a first step towards creating fully functional blood stem cells in test tubes. (lu.se)
Blood stem cells lose important characteristics when they are cultured, while skin cells are both easily accessible and can be easily expanded in test tubes. (lu.se)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

Amoeboid cell motility plays a vital role in physiological and pathological processes ranging from embryonic development and immunological defense to tumor metastasis. (fsu.edu)

In the last few decades, studies have revealed the presence of CD34 expression on other types of cells with non-hematopoietic origins, such as interstitial cells, endothelial cells, fibrocytes, and muscle satellite cells. (bvsalud.org)
A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. (thermofisher.com)
Leukocyte depletion of transfused blood products may prevent alloimmunization to platelets and should be used in patients expected to need platelet transfusions during multiple courses of chemotherapy or for candidates for hematopoietic cell transplants. (msdmanuals.com)

Methods CT26 models were treated with anti-PD-1 on days 3, 6 and 9 following subcutaneous tumor implantation generating variable responses during early tumor development. (bmj.com)
In this article, we review recently developed methods that enable functional genomic analysis at the single-cell or multi-cell level. (ejbiotechnology.info)
To achieve our aim, we use methods from species evolution (phylogenetics) to reconstruct the evolutionary history of individual tumours, from its founder cell up to clinical presentation, over treatment periods and at relapse. (lu.se)

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and is divided into three subtypes according to pathogenesis: adenocarcinoma, squamous cell carcinoma and large cell carcinoma [ 2 ]. (oncotarget.com)
An important development in cancer research over the past 2 decades has been the recognition that genetic changes drive the pathogenesis of tumors of both adulthood and childhood. (medscape.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

High avidity T cells (Tet hi ), if present, were only found in progressing PD-1 refractory tumors. (bmj.com)
Consequently, safer and more efficient therapy options are urgently needed, especially for the treatment of metastatic tumors refractory to conventional treatments. (helsinki.fi)

BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Microarrays were prepared in tumor biology. (lu.se)

We are very proud to demonstrate that our high-throughput functional T cell screening approach proves to be a key tool in development of TCR-based cancer immunotherapies that can target a new class of TSAs based on peptides derived from non-coding genetic regions expressed by tumor cells. (medigene.com)
Genetic alterations of BCL2 (B-cell CLL/lymphoma 2), MYC [v-myc myelocytomatosis viral oncogene homolog (avian)] and CCND1 (cyclin D1) are the most frequently found alterations in human B-cell lymphomas. (haematologica.org)
Kidney tumors from stilbene estrogen ( diethylstilbestrol )-treated Syrian hamsters were screened for somatic genetic alterations by Random Amplified Polymorphic DNA-polymerase chain-reaction (RAPD-PCR) fingerprinting. (diethylstilbestrol.co.uk)
Single-cell genetic analysis of clonal dynamics in colorectal adenomas indicates CDX2 gain as a predictor of recurrence. (cancerindex.org)
We make genetic and functional maps of cancer cell evolution in patients by analysing multiple tumour samples from the same patient. (lu.se)
Through the cloning of Dolly, we learned that the cell nucleus contains all the genetic information needed for the cell to develop into any type of cell. (lu.se)
A Gradual Transition Toward Anaplasia in Wilms Tumor Through Tolerance to Genetic Damage. (lu.se)

Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)
However, analogous advanced treatments are not available for patients with lung squamous cell carcinoma (lung SCC). (oncotarget.com)
In contrast, there is a lack of effective therapeutic options to treat patients with lung squamous cell carcinoma (lung SCC) [ 3 ]. (oncotarget.com)
Kelly, Shane (2016) Proteomic characterisation of clonal populations from a humanlung carcinoma cell line. (dcu.ie)

It specifically negatively regulates the PI3K-AKT signaling pathway to induce cell cycle arrest. (medscape.com)
The discovery of a novel ligand for PDGFRβ, platelet-derived growth factor (PDGF)-DD, opened the possibility of a previously unidentified signaling pathway involved in tumor development. (lu.se)

Simultaneously, they also activate the dormant host's immune system to fight against the tumor. (helsinki.fi)
TIL therapy is a type of adoptive cellular therapy leveraging the patient's own immune system to treat tumors. (biomedcentral.com)
Pereira's research also involves reprogramming skin cells into dendritic cells, which are the sentinels of the immune system. (lu.se)
However, as tumors evade immune system detection many patients or tumor types do not respond. (lu.se)

While GB tumors are frequently infiltrated by natural killer (NK) cells, these are actively suppressed by the GB cells and the GB tumor microenvironment. (frontiersin.org)
Nevertheless, the composition of the immunologic tumor microenvironment undergoes changes upon radiotherapy, chemotherapy, or even after anti-angiogenic therapy ( 6 ). (frontiersin.org)
Also, we compare patterns of evolution to parameters of the tumour microenvironment in order to find out how surrounding factors shape the cancer genome through selection. (lu.se)

However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
RAPD-PCR loci showing decreased and increased intensities in tumor tissue DNA relative to control DNA indicate that loci have undergone allelic losses and gains, respectively, in the stilbene estrogen-induced tumor cell genome . (diethylstilbestrol.co.uk)
The presence or absence of the amplified DNA fragments indicate homozygous insertions or deletions in the kidney tumor DNA compared to the age-matched normal kidney tissue DNA. (diethylstilbestrol.co.uk)
The multi‑dimensional nature of their roles in cellular homeostasis, cell‑to‑cell and tissue‑to‑tissue communication at the level of the organism, as well as their actions on the holobiome (intra‑/interspecies interaction), have garnered the interest of a large number of researchers. (spandidos-publications.com)
Endocrinology, by definition, focuses on homeostatic, and cell‑to‑cell and tissue‑to‑tissue communication mechanisms. (spandidos-publications.com)
Exosomes, via their cargo or surface composition, are signals/mediators of systemic homeostasis and stress for specific cell-to-cell or tissue-to-tissue communication ( 5 ). (spandidos-publications.com)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

Medigene concentrates on the development of personalized T cell-based therapies, with associated projects currently in pre-clinical and clinical development. (medigene.com)
To functionally interrogate mechanisms of drug resistance, we have developed a robust infrastructure for generating patientderived cell lines and mouse patient-derived xenograft (PDX) models from lung cancer patients treated with targeted therapies at the MGH Cancer Center. (massgeneral.org)
These models have enabled functional screens to identify novel mechanisms of acquired resistance and testing of novel next-generation therapies to overcome them. (massgeneral.org)
Our TCR screening prototype will facilitate immunotherapeutic screening and development of T cell therapies. (rsc.org)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
Decreased blood concentrations of red blood cells (RBCs), white blood cells (WBC), especially granulocytes, and platelets result from diverse systemic cancer therapies, especially conventional chemotherapy drugs, and radiation therapy. (msdmanuals.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

In cancer patients dendritic cells may become dysfunctional or excluded from tumors. (lu.se)
Therefore, new ways are needed to reprogram other cells into immune cells with the same capacity as the "natural" dendritic cells. (lu.se)

Tetramer staining was performed to determine the frequency and avidity of CD8 + T cells targeting the tumor-specific epitope GSW11 and confirmed with tetramer competition assays. (bmj.com)
Although our strategy may not catch all relevant clones in the principal individual sample our research targets the essential tumorigenic small fraction as practical assays for the majority population never have been created. (molecularcircuit.com)

However, spontaneous antitumor CD8+ T cell immunity in peripheral blood and tumors was restricted to a few clonal neoantigens featuring an oligo-/monoclonal T cell-receptor (TCR) repertoire. (nih.gov)

Medigene's high-throughput screening technology identified ten peptides as immunogenic and able to induce specific T cell responses. (medigene.com)

the 5-year patient survival is about 8% ( 1 ) due to the late-stage disease diagnosis, tangible tumor resistance to therapy ( 2 ) and aberrant post-treatment tumor relapse ( 3 ). (amegroups.org)
Nevertheless, the successful application of TIL therapy currently is still limited to several types of tumors. (biomedcentral.com)
Our work has also shed light on how cancer cells adapt and evolve during the course of therapy and we are currently working to identify targetable vulnerabilities in cancer cells that can be exploited to prevent resistance from developing in the first place. (massgeneral.org)
One potential software of this strategy would be the advancement of anticipatory therapy fond of the most intense relapse-initiating clones determined during individual diagnosis. (molecularcircuit.com)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)

To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is essential for scientists and physicians to develop a strategy to identify genomic expression profiles for a single-cell or for small numbers of cells. (ejbiotechnology.info)
Studies have revealed a heterogeneous subclonal architecture to lung cancer, which contributes to the persistence of the disease by allowing the tumour to metastasise to distant sites, utilising the different phenotypic characteristics of the heterogeneous population. (dcu.ie)

Expression of this gene has been shown to be induced by gamma interferon in some cancer cells. (wikipedia.org)
DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53. (cancerindex.org)
Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). (cancerindex.org)
We look forward to learning whether this novel class of TSAs will form a strong basis for T cell-based immunotherapies that can reach more patients who are in need of new therapeutic options to treat cancer. (medigene.com)
However, the impact of miR-145-3p (the passenger strand) on cancer cells is still ambiguous. (oncotarget.com)
O]ur work highlights that an essential subset of [single-nucleotide variants] currently identified as passengers nonetheless may not merely be going for the ride and may in fact have important functional roles in driving cancer," senior and corresponding author Mark Gerstein, a researcher at Yale, and his colleagues wrote. (genomeweb.com)
Since then, it has been characterized as the prototypical cell-surface marker for prostate cancer and has been the subject of intense clinical inquiry. (omicsdi.org)
Future study of PSMA in prostate cancer might focus on its intracellular functions and possible role in tumor neurogenesis.Twenty years from its discovery, PSMA represents a viable biomarker and treatment target in prostate cancer. (omicsdi.org)
The aim of this study was to investigate the prognostic effect of CD34 + CD38- leukemia cells in this childhood cancer. (bvsalud.org)
Combination of IL-2/15/21 can enhance TIL expansion in lung and colorectal cancer and promote CD8+ T cell percentage as well as TCR clone diversity compared to IL-2 alone [ 13 ]. (biomedcentral.com)
2003. Caspase-8 and apoptosis-inducing factor mediate a cytochrome c-independent pathway of apoptosis in human colon cancer cells induced by the dietary phytochemical chlorophyllin. . (oregonstate.edu)
OR 2.2 suggesting that known cancer pathways are involved in differential drug response of clones of GBM-482. (molecularcircuit.com)
Our new synergism-based method is useful for the identification of synergistic gene combinations in tumor development, and may expand our systemic understanding of a wide range of cancer-causing elements. (haematologica.org)
Landscape of tumor suppressor long noncoding RNAs in breast cancer. (cancerindex.org)
The landscape and biological functions of tumor suppressor long noncoding RNAs in breast cancer are still unknown. (cancerindex.org)
Next, the expression model, prognostic roles, potential biological functions and epigenetic regulation of tumor suppressor long noncoding RNAs were investigated and validated in the breast cancer and pancancer cohorts. (cancerindex.org)
Finally, EPB41L4A-AS2 was selected to validate our novel finding, and the tumor suppressive roles of EPB41L4A-AS2 in breast cancer were examined. (cancerindex.org)
This work enlarges the functional landscape of known long noncoding RNAs in human cancer and provides novel insights into the suppressive roles of these long noncoding RNAs. (cancerindex.org)
First, we isolated resistant clones from a human prostate cancer cell line, PC3, after repeated exposure to Ad-REIC. (elsevierpure.com)
Hypoxic cancer cells are known to be relatively resistant to radiation killing compared with well oxygenated healthy cells [2,3,7-9]. (who.int)
Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
One main cause of cancer death is the emergence of treatment resistance through clonal evolution of cancer cells. (lu.se)
arations verified the presence of cancer cells in all samples. (lu.se)
Загальні відомості про терапію злоякісних новоутворень Curing cancer requires eliminating all cells capable of causing cancer recurrence in a person's lifetime. (msdmanuals.com)

Tet lo demonstrated precursor exhausted or progenitor T cell phenotypes marked by higher expression of Tcf-1 and T-bet, and lower expression of the exhaustion markers CD39, PD-1 and Eomes compared with Tet hi , whereas Tet hi cells were terminally exhausted. (bmj.com)
p53 is a transcription factor whose expression is increased by DNA damage and blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. (ejbiotechnology.info)
2003. Mutational analysis of Ctnnb1 and Apc in tumors from rats given 1,2-dimethylhydrazine or 2-amino-3-methylimidazo[4,5-f]quinoline: mutational 'hotspots' and the relative expression of beta-catenin and c-jun. . (oregonstate.edu)
The presence of a subclonal population of tumor cells characterized by PDGFRβ expression was further validated in a cohort of human PanNET. (lu.se)
For example the well-defined GBM oncogenes hepatocyte growth factor receptor (MET) and show reduced expression in the TMZ-sensitive clone of GBM-482. (molecularcircuit.com)
Mechanistically, EPB41L4A-AS2, acting at least in part as a tumor suppressor, upregulated tumor suppressor gene expression. (cancerindex.org)
Infection efficiency of the adenovirus vector and expression level of REIC/Dkk-3 in the resistant clones were similar to those in the parental PC3 cells. (elsevierpure.com)
Differential display analysis of gene expression revealed the overexpression of translation elongation factor-1 delta sub-unit in BALB/c-3T3 cells transformed with cadmium chloride compared with the non- transformed control cells. (cdc.gov)
Expression of the antisense translation elongation factor-l delta mRNA resulted in marked reversal of the oncogenic potential of the cadmium -transformed BALB/c- 3T3 cells. (cdc.gov)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

Myoglobin-reactive T cell clones. (uthscsa.edu)

To circumvent these obstacles, considerable effort has been invested in attempting to derive ESC-like cells by reprogramming somatic cells to an embryonic state. (biomedcentral.com)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
Several experimental strategies have been developed to derive iPSCs from differentiated somatic cells (summarized in Figure 1 ). (biomedcentral.com)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

Flow cytometric analysis of Chondroitin Sulfate expressed on M21 human melanoma cells. (bdbiosciences.com)
Clone 9.2.27 has been shown to react with human melanoma cells, glioma cells, and proliferating brain endothelial cells. (bdbiosciences.com)

Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. (ancell.com)

The traditional procedure of TIL production is assayed for specific tumor recognition and usually takes 6-8 weeks. (biomedcentral.com)
Here, we use droplet microfluidics for functional screening and real-time monitoring of single TCR T cell activation upon recognition of target tumor cells. (rsc.org)

We have now expanded this study to evaluate the efficacy of PD-1 blockade in patients with advanced mismatch repair-deficient cancers across 12 different tumor types. (stanfordhealthcare.org)
We have recently shown that an adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. (elsevierpure.com)

We previously showed that murine embryonic fibroblasts (MEFs) from TNF receptor-associated factor 2 (Traf2) and Traf5 double-deficient (double knockout (DKO)) mice were highly susceptible to TNF-induced cell death. (elsevierpure.com)

Anatomy25

Organisms11

Diseases70

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes53

Disciplines and Occupations1

Information Science4

Health Care5

Solid tumors4

Proliferation21

Genes23

Antigen6

Functions as a tumor suppressor1

Proteins14

Antigens6

Tissues13

Lymphocytes4

Protein22

Immunogen1

Vivo12

Biomarkers6

Progression2

Specificity1

Malignant6

Inhibiting tumor growth2

Human tumors1

Molecular6

Various tumors2

Liver tumors1

Transplantation9

Analyses5

Necrosis Factor2

Antibodies1

Expressed in pancreatic1

Carcinogenesis2

Fetal3

Autologous3

Progenitor cells2

Immunotherapy6

Lymph node metastasis1

Specimens5

Flow cytometry1

Differentiation5

Antibody7

Mutations7

Vitro2

Breast tumor1

Molecules8

Cytokine1

Subpopulations3

Stem cells11

Metastasis1

Hematopoietic3

METHODS3

Pathogenesis3

Refractory2

Biology2

Genetic7

Carcinoma5

Signaling pathway2

Immune system4

Microenvironment3

Tissue7

Therapies8

Dendritic Cells2

Assays2

Monoclonal1

Induce1

Therapy6

Heterogeneous2

Cancer24

Expression11

Reactive1

Somatic4

Melanoma cells2

Molecule1

Recognition2

Cancers2

Embryonic1