• In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and were investigated as scaffolds for cancer cells in vitro culture to simulate tumor microenvironment. (edu.au)
  • Thus, addressing the presence of immunosuppressive myeloid cell populations in the periphery and tumor microenvironment of patients with cancer is likely to be required for effective NK cell-based immunotherapy. (elifesciences.org)
  • Then, 200 ng of RNA per sample were loaded and further analyzed according to the manufacturer's recommendation on a NanoString nCounter® system using the Breast Cancer 360 code set, which is comprised of 18 housekeeping genes and 752 target genes covering key pathways in tumor biology, microenvironment, and immune response. (scilifelab.se)
  • However, a major limitation of this emerging technology is the absence of a tumor microenvironment that includes immune and stromal cells . (bvsalud.org)
  • By integrating cellular components of the tumor microenvironment , including T cells , into tumor organoid cultures , immuno-oncology has embraced this technology , which is rapidly advancing. (bvsalud.org)
  • Increasing efforts have been made in developing co-culture assays with sophisticated materials and platforms aiming to mimic the tumor microenvironment (TME), but its complexity makes it difficult to develop the ideal model. (maastrichtuniversity.nl)
  • Thus, the APJ system in fibroblasts in the tumor microenvironment may be a promising therapeutic target. (spandidos-publications.com)
  • The tumor microenvironment consists of various cell types and fibroblasts in the tumor tissue, called cancer-associated fibroblasts (CAFs), which play pivotal role in tumor progression ( 7 , 8 ). (spandidos-publications.com)
  • The mutational inactivation of the TP53 gene in tumor cells has been reported to affect not only tumor cells but also the surrounding cells in the tumor microenvironment and to promote tumor-stromal activation and subsequent tumor growth ( 11 , 12 ). (spandidos-publications.com)
  • Tumor growth is sustained by a highly diverse microenvironment, the TME. (promocell.com)
  • Successive assays revealed that some compounds were endowed with a promising anti-proliferative effect against HCT116 cells, alone or stimulated by serotonin as a pro-inflammatory factor in order to mimick an inflamed model of cancer cell microenvironment. (amrita.edu)
  • We are interested in how gene expression is regulated in the brain and how this process influence neurodegenerative diseases, psychiatric disorders and brain tumors. (lu.se)
  • Tumor cells with stem cell characteristics are thought to be responsible for therapeutic resistance in brain tumors. (lu.se)
  • Using mouse models of glioma and primary human glioma cultures, we aim to characterize phenotypic intratumoral heterogeneity specifically with regards to radiation resistant stem-like tumor cells, the molecular signaling pathways underlying therapeutic resistance, and microenvironmental control over tumor cell phenotypes with the overall goal of developing novel therapeutic strategies targeting therapy-resistant cells in malignant brain tumors. (lu.se)
  • Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
  • SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)
  • 2017. Divergent roles of p120-catenin isoforms linked to altered cell viability, proliferation, and invasiveness in carcinogen-induced rat skin tumors. . (oregonstate.edu)
  • Data from experimental studies in animals and cultured mammalian cells indicate that laboratory-generated roofing asphalt fume condensates are genotoxic and cause skin tumors in mice when applied dermally. (cdc.gov)
  • Here, we examined the anti-lung-metastatic effects and the mechanism of lycopene in combination with sorafenib in C57BL/6 mice xenografted with Lewis lung carcinoma (LLC) cells. (frontiersin.org)
  • The results showed that lycopene reduced the number of metastatic tumors in the lungs, which was further suppressed by the combined treatment with sorafenib. (frontiersin.org)
  • In anti-metastatic studies, lycopene has been found to inhibit the metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice ( 17 ). (frontiersin.org)
  • spread of metastatic cells [3]. (researchgate.net)
  • In addition, we differentiate for the first time the contributions of ERBB1 and ERBB2 to the key metastatic properties of in vivo tumor cell invasion and intravasation. (aacrjournals.org)
  • There is an unmet clinical need to extract living circulating tumor cells (CTCs) for functional studies and in vitro expansion to enable drug testing and predict responses to therapy in metastatic cancer. (lu.se)
  • TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
  • Breast cancer is one of the most common primary tumors to develop associated brain metastases ( 1 , 2 ), and while recent progress in cancer therapy has improved control of primary breast tumors, methods to effectively treat brain metastases from breast cancer remain inadequate. (jcancer.org)
  • Three-dimensional (3D) cells in vitro culture are becoming increasingly popular in cancer research because some important signals are lost when cells are cultured in a two-dimensional (2D) substrate. (edu.au)
  • Their properties and ability to support normal growth of cancer cells were evaluated. (edu.au)
  • In particular, the human breast cancer cell line (MDA-MD-231) was seeded into BC/gelatin scaffolds to investigate their potential in 3D cell in vitro culture. (edu.au)
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
  • Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
  • Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • Overall, the present study demonstrates that lycopene in combination with sorafenib additively inhibits the lung metastasis of tumor, indicating lycopene has potential as an adjuvant for sorafenib in cancer treatment. (frontiersin.org)
  • During the metastasis, cancer cells in situ move to other tissues and organs from the blood circulation or the lymphatic system through proliferation, adhesion, invasion, and migration. (frontiersin.org)
  • To this topic, lycopene had been demonstrated to have synergism from the combination of oxaliplatin in a human ovarian cancer cell line ( 20 ), suggesting that the adjuvant property on the anticancer of lycopene. (frontiersin.org)
  • Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
  • Lung cancer is divided into two major groups: non small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (scielo.br)
  • 2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)
  • 2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
  • Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is a glycoprotein that has limited expression in normal adult tissues, but is overexpressed in carcinomas of the gastrointestinal tract, the genitourinary and respiratory systems, and breast cancer. (nih.gov)
  • We tested an essential-oil derivative, camphor white oil (CWO), for anti-tumor activity in a mouse model of keratinocyte-derived skin cancer. (biorxiv.org)
  • Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)
  • PD-L1 CAR haNKs reduced levels of macrophages and other myeloid cells endogenously expressing high PD-L1 in peripheral blood from patients with head and neck cancer. (elifesciences.org)
  • 3D Cell Culture: Tumor-specific cancer treatments, org. (selectscience.net)
  • In recent years, patient-derived tumor organoid culture has been developed as an efficient and economic model to mimic and recapitulate the original tumor tissue, in contrast to conventional cancer cell lines and patient-derived xenografts (Drost & Clevers, 2018). (hku.hk)
  • Notably, PDOs from CRT-treated patients show enhanced re-growth after recovery from cisplatin treatment, indicating a candidate cancer stem cell (CSC) sub-population enriched by neoadjuvant CRT. (hku.hk)
  • This record is a collection of Whole-genome sequencing (WGS), RNA sequencing (RNA-seq), NanoString's nCounter® Breast Cancer 360 (BC360) Panel and cell viability assay data, generated as part of the study " Breast cancer patient-derived whole-tumor cell culture model for efficient drug profiling and treatment response prediction " by Chen et al. (scilifelab.se)
  • The WGS dataset contains raw sequencing data (BAM files) from tumor scraping cells (TSCs) at the time of surgical resection, derived whole-tumor cell (WTC) cultures from each patient's specimen, and normal skin biopsy for germline control, from five (5) breast cancer (BC) patients. (scilifelab.se)
  • Novel cancer treatment strategies take advantage of tumor-induced vascularisation by combining standard chemotherapeutic agents with angiogenesis-inhibiting agents. (biomedcentral.com)
  • The use of patient -derived tumor tissues and cells has led to significant advances in personalized cancer therapy and precision medicine . (bvsalud.org)
  • The three-dimensional tumor organoids derived from self -organizing cancer stem cells are valuable ex vivo models that faithfully replicate the structure, unique features, and genetic characteristics of tumors . (bvsalud.org)
  • This review focuses on the current applications of organoid - T cell co-culture models in cancer research and highlights the remaining challenges that need to be addressed for its broader implementation in anti- cancer therapy . (bvsalud.org)
  • The availability of the soft tissue melanoma cell line, the SCID mouse xenograft tumour system as well as autologous TILs described herein would provide useful materials for identifying T-cell-defined antigens as well as a model system for devising individualised cancer biotherapeutic strategies. (tmu.edu.tw)
  • This cell line can also be used for further studies aimed at uncovering the histogenesis of this rare cancer. (tmu.edu.tw)
  • T cell immunotherapies have shown great promise in patients with advanced cancer disease, revolutionizing treatment. (maastrichtuniversity.nl)
  • IPA analysis of the 280 significantly differentially expressed genes in the whole blood was suggestive of increased hematopoiesis and cell development, activation of cancer/tumor development pathways, and atopy. (cdc.gov)
  • Cancer‑associated fibroblasts (CAFs) are pivotal in tumor progression. (spandidos-publications.com)
  • TP53‑deficiency in cancer cells is associated with robust stromal activation. (spandidos-publications.com)
  • The role of exosomes collected by ultracentrifugation were also analyzed as mediators of p53 expression in cancer cells and APJ expression in fibroblasts. (spandidos-publications.com)
  • APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
  • MicroRNA 5703 enriched in exosomes derived from HCT116 sh p53 cells inhibited APJ expression, and inhibition of miR‑5703 diminished APJ suppression in fibroblasts caused by cancer cells. (spandidos-publications.com)
  • APJ suppression from a specific microRNA in cancer cell‑derived exosomes induced CAF‑like properties in fibroblasts. (spandidos-publications.com)
  • TP53 is a significant tumor suppressor gene, and its somatic mutations are one of the most frequent alterations in ~50% of all human cancers, including colorectal cancer ( 10 ). (spandidos-publications.com)
  • Cancer/testis antigen MAGEC2 (also known as HCA587) is highly expressed in a wide variety of tumors and plays an active role in promoting growth and metastasis of tumor cells. (biomedcentral.com)
  • However, little is known for the regulation of MAGEC2 expression in cancer cells. (biomedcentral.com)
  • Our findings show that TRIM28 is necessary for MAGEC2 expression in cancer cells, and TRIM28 may serve as a new potential target for immunotherapy of cancer. (biomedcentral.com)
  • Using in vivo mouse models of breast cancer, we test the effects of ERBB1 and ERBB2 inhibitors AC480 and lapatinib, ERBB1 inhibitor gefitinib, and ERBB2 inhibitor AG825 on in vivo tumor cell invasive properties in mammary fat pad tumors. (aacrjournals.org)
  • In mice, a fatal brainstem tumor was cleared by injecting it with engineered T cells that recognized the cancer and targeted it for destruction. (stanford.edu)
  • In mice whose brainstems were implanted with human DIPG, engineered immune cells known as chimeric antigen receptor T cells - or CAR-T cells - were able to eliminate tumors, leaving very few residual cancer cells. (stanford.edu)
  • When the brains of the mice were examined via immunostaining after treatment, the animals had, on average, a few dozen cancer cells left, compared with tens of thousands of cancer cells in animals that received a control treatment. (stanford.edu)
  • As a cancer immunotherapist, what gets me really excited is when you take an established tumor and you make it disappear," said Crystal Mackall , MD, professor of pediatrics and of medicine and the study's other senior author. (stanford.edu)
  • In CAR-T therapies now used in humans, some of the patient's own immune cells are removed, engineered to attack a surface antigen on the cancer cells, and returned to the patient's body, where they target the cancer cells for destruction. (stanford.edu)
  • Scientists have known for decades that GD2 levels on some other forms of cancer are very high, but its discovery on this tumor came as a surprise, Mackall said, adding, "It was hiding in plain sight, and we didn't know. (stanford.edu)
  • Other CAR-T cells that were tuned to different molecular targets also did not kill the DIPG cancer cells. (stanford.edu)
  • If p53 is inactivated, as it is in over half of all human cancers, checks and balances on cell growth fail to operate, and body cells start to accumulate mutations, which ultimately may lead to cancer. (news-medical.net)
  • Many chemotherapeutical drugs used to treat cancer exert their biological effects on tumor cells through activation of the p53 pathway. (news-medical.net)
  • Unless promptly and properly repaired, these DNA breaks can let cell division spiral out of control, ultimately causing cancer. (news-medical.net)
  • Immune cell infiltrate (in deep red) around senescent cancer cells (large nuclei marked in blue). (irbbarcelona.org)
  • Senescent tumor cells, mouse pancreatic cancer cell culture. (irbbarcelona.org)
  • Vaccination with senescent cells significantly reduces the development of tumours in experimental models of melanoma and pancreatic cancer. (irbbarcelona.org)
  • Cancer cells have a series of features that allow the immune system to identify and attack them. (irbbarcelona.org)
  • This means that immune cells cannot reach the cancer cells to remove them. (irbbarcelona.org)
  • The scientific community has been working for years to increase the effectiveness of the immune system against cancer by using vaccines based on dead tumour cells. (irbbarcelona.org)
  • Scientists at IRB Barcelona, led by ICREA researcher Dr. Manuel Serrano , and Dr. Federico Pietrocola , now at the Karolinska Institutet, in Sweden, have studied how inducing senescence in cancer cells improves the effectiveness of the immune response to a greater degree than the dead cancer cells. (irbbarcelona.org)
  • After vaccinating healthy mice with senescent cancer cells and then stimulating the formation of tumours, the researchers observed that the animals did not develop cancer or that the number that do is significantly reduced. (irbbarcelona.org)
  • Our results indicate that senescent cells are a preferred option when it comes to stimulating the immune system against cancer, and they pave the way to considering vaccination with these cells as a possible therapy," explains Dr. Serrano , head of the Cellular Plasticity and Disease lab at IRB Barcelona. (irbbarcelona.org)
  • The researchers tested the technique in animal models of melanoma, a type of cancer characterised by high activation of the immune system, and also in pancreatic cancer models, which present strong barriers against immune cells. (irbbarcelona.org)
  • Prophylactic vaccination therapy with senescent cancer cells was effective against both types of tumors. (irbbarcelona.org)
  • They also complemented the study with tumour samples from cancer patients and confirmed that human cancer cells also have a greater capacity to activate the immune system when they are previously rendered senescent. (irbbarcelona.org)
  • In the context of cancer, the researchers led by Dr. Serrano have discovered that senescent cells, due to their characteristics, are a good option for activating the immune system and improving its response to the tumour. (irbbarcelona.org)
  • A team of researchers has developed a potential new therapy that may work in two distinct ways to attack tumors, by directly killing cancer cells and immune cells that can suppress the anti-cancer immune response. (cancer.gov)
  • Here, we present a novel two-step acoustophoresis (A2) method for isolation of unfixed, viable cancer cells from red blood cell (RBC) lysed whole blood. (lu.se)
  • This acoustofluidic step enriches viable cancer cells in a central outlet, but a significant number of white blood cells (WBCs) remain in the central outlet fraction due to overlapping acoustophysical properties of these viable cells. (lu.se)
  • Using samples with 10 000 DU145 cells, we obtained 459 ± 188-fold depletion of WBC and 42% recovery of viable cancer cells. (lu.se)
  • The novel A2 method provides extensive elimination of WBCs combined with the gentle recovery of viable cancer cells suitable for downstream functional analyses and in vitro culture. (lu.se)
  • Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
  • Therefore CAR T cells and TCR T cells may represent complementary approaches to cancer therapy. (msdmanuals.com)
  • CAR T cell immunotherapy for human cancer. (msdmanuals.com)
  • A cytology exam of pleural fluid is a laboratory test to detect cancer cells and certain other cells in the fluid from the area that surrounds the lungs. (medlineplus.gov)
  • A cytology exam is used to look for cancer and precancerous cells. (medlineplus.gov)
  • Low Wnt-5a expression in a primary breast tumor is associated with shorter recurrence free survival, suggesting that Wnt-5a expression acts to restrict breast cancer metastasis. (lu.se)
  • Finally, we found that phospho-DARPP-32 inhibited the activity of the focal adhesion kinase (FAK) in MCF-7 breast cancer cells, and that MCF-7 cells expressing phospho-DARPP-32 displayed less filopodia formation. (lu.se)
  • The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant adhesion, proliferation, ingrowth and differentiation. (edu.au)
  • Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
  • HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
  • Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)
  • MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
  • IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
  • or physiological or as a description of key events and processes starting toxic effects that affect cell proliferation. (cdc.gov)
  • Experimental in vitro and in vivo research demonstrated decreased cell proliferation in desmoid cell cultures and small tumors when COX-2 was blockaded with pharmacologic agents. (medscape.com)
  • The presence of tumor cells induced blastogenesis, elevation of the proportion of Fc receptor positive cells and generated cytotoxic cells to the sensitizer tumor. (ui.ac.id)
  • The precursor of cytotoxic cells did not have Fc or C3 receptors since nylon wool column passed fractions depleted from these cells by elimination of EA or EAC rosettes were also activated. (ui.ac.id)
  • Boron neutron capture therapy (BNCT) is a promising method for treating tumors, in particular, infiltrative malignant tumors, due to the selective destruction of tumor cells without damaging the surrounding normal tissues. (isnct.net)
  • Western blotting was employed to determine NGF level in PC and paracarcinoma tissues and in PC cell lines as well as pancreatic ductal epithelial cells. (hindawi.com)
  • NGF level was preeminently higher in PC tissues and cell lines than in paracarcinoma tissues and normal pancreatic epithelial cell lines. (hindawi.com)
  • Tumors are generated from human tumor cell lines or tissues, then dissected and digested using an enzyme cocktail of DNase I (Sigma 10104159001), trypsin inhibitor (Sigma T9253-5G), and papain (Worthington LS003126) into a single cell suspension. (stanford.edu)
  • Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
  • Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. (elifesciences.org)
  • Generally, it is believed that there are four main pathways between tumor invasion and metastasis, including local invasion, blood, lymphatic, and seeding metastasis [ 6 ]. (hindawi.com)
  • In addition to these four pathways, tumors can undergo infiltration around nerve fibers and metastasis along nerves, that is, perineural invasion (PNI), which refers to the phenomenon of perineural invasion by tumor cells filling the perineurial space, wrapping around nerves in a continuous concentric sheath-like pattern, infiltration, and metastasis of extension along nerves around nerve fibers or into perineurium within the perineurium [ 7 ]. (hindawi.com)
  • Treatment of syngeneic tumors resulted in CD8 and PD-L1-dependent tumor rejection or growth inhibition and a reduction in myeloid cells endogenously expressing high levels of PD-L1. (elifesciences.org)
  • Treatment of xenograft tumors resulted in PD-L1-dependent tumor growth inhibition. (elifesciences.org)
  • Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
  • Our studies were designed to evaluate the effect of ERBB inhibition on tumor cell motility and intravasation in vivo using clinically relevant small-molecule inhibitors. (aacrjournals.org)
  • ERBB1 and ERBB2 inhibition rapidly (within 3 h) inhibits both tumor cell motility and intravasation. (aacrjournals.org)
  • Using gefitinib, ERBB1 inhibition rapidly inhibits tumor cell motility and invasion but not intravasation, whereas ERBB2 inhibition by AG825 rapidly blocks intravasation. (aacrjournals.org)
  • ERBB1 and ERBB2 inhibition can rapidly block tumor cell invasive properties. (aacrjournals.org)
  • These results indicate that ERBB inhibition should be considered for blocking other tumor cell malignant properties besides growth. (aacrjournals.org)
  • Koontongkaew S, Monthanapisut P, Saensuk T: Inhibition of arachidonic acid metabolism decreases tumor cell invasion and matrix metalloproteinase expression. (karger.com)
  • DARPP-32 mediated inhibition of cell migration proved to be dependent on DDR1 expression, consolidating a functional relevance of the interaction between DARPP-32 and DDR1. (lu.se)
  • In addition, we found that Wnt-5a could directly trigger a cAMP response that resulted in phosphorylation of DARPP-32 and stimulation with Wnt-5a was necessary for DARPP-32 mediated inhibition of cell migration in wound healing assay. (lu.se)
  • Total population and T cell enriched fractions of rat spleen were cultivated in diffusion chambers implanted intraperitoneally to mice and rats. (ui.ac.id)
  • Subsequently, we used a model of neuroinvasion in nude mice to assess the effect of NGF in vivo on tumor nerve invasion as well as on nociceptive transmission. (hindawi.com)
  • Cell suspensions of whole spleens and tumors containing splenic or tumor-infiltrating effector T cells of mice bearing Lewis lung carcinoma (LLC) or CT26 colon carcinoma tumors treated with radiation alone or in combination with immunotherapies were used for co-culture. (maastrichtuniversity.nl)
  • We demonstrated that splenocytes and tumor-infiltrating T cells derived from mice treated with combination therapy were able to kill approximately 50% of target cells after 48 h of co-culture. (maastrichtuniversity.nl)
  • The study , which was published online April 16 in Nature Medicine, represents the first time a severe human brainstem tumor, diffuse intrinsic pontine glioma, has been eradicated in mice. (stanford.edu)
  • However, some mice experienced dangerous levels of brain swelling, a side effect of the immune response triggered by the engineered cells, the researchers said, adding that extreme caution will be needed to introduce the approach in human clinical trials. (stanford.edu)
  • Crystal Mackall says she is encouraged that the work in mice showed that engineered immune cells called CAR-T cells were able to eradicate the tumors in mice. (stanford.edu)
  • Next, the team tested the GD2 CAR-T cells in mice whose brainstem was implanted with human DIPG tumors, an experimental system that Monje's lab pioneered. (stanford.edu)
  • Instead of accumulating in mouse cells and halting cell division in the genetically engineered mice, the altered p53 protein performed flawlessly: it was unstable when no DNA damage was present and was stable and fully functional when needed to halt the cycle cell to repair DNA damage or to induce apoptosis. (news-medical.net)
  • Stimulation of d endritic cell activation was also able to promote a potent antitumor effect with 70% complete response and increased overall survival of mice implanted with an orthotopic head & neck tumor. (unil.ch)
  • Appropriate number of cells will be prepared into single cell suspension in 50-100μL volume (PBS:matrigel=1:1) and injected subcutaneously on the flank or the abdominal area of the mice. (stanford.edu)
  • Tumor tissue or cells can be implanted into the specific orthotopic sites of the mice. (stanford.edu)
  • Cells are mixed with matrigel (Corning 356237, non-phenol red, low growth factor) and injected into mice for passaging at 2-5million cells per 50μl SQ. Additional cells can be frozen down for future usage of P1 and P2 passages. (stanford.edu)
  • Tumor fragments from tumor growing in donor mice will be passaged to recepient mice. (stanford.edu)
  • Our technical expertise include lentiviral vectors, transgenic mice and stem cells cultures. (lu.se)
  • I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)
  • More importantly, MDA-MD-231 cells cultured in BC/gelatin scaffolds retained triple-negative receptor expression, demonstrating that BC/gelatin scaffolds could be used as ideal in vitro culture scaffolds for tumor cells. (edu.au)
  • Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
  • Results: In the current project, we have established and biobanked a panel of ESCC PDO cultures in vitro from Chinese ESCC treatment-naïve patients undergoing an endoscopic examination or upfront surgery, or patients undergoing neoadjuvant chemoradiotherapy (CRT) followed by surgery in Hong Kong. (hku.hk)
  • To reveal that the NGF-TrkA signaling pathway was closely associated with PC PNI, in vitro neuroinvasion model was established by using MiaPaCa-2 cells via coculturing DRG cells in Matrigel. (hindawi.com)
  • As linear correlations exist between expression levels of angiogenic factors under normoxic and hypoxic conditions in vitro , we propose that explanted primary cells may be used to probe the in vivo hypoxic environment. (biomedcentral.com)
  • In vitro studies have shown that stromal cells cultured in hypoxic growth conditions secrete higher levels of critical angiogenesis-inducing factors than cells cultured in normoxic conditions [ 3 ]. (biomedcentral.com)
  • MST-1 cells grown in vitro were heterogeneous in morphology, ranging from floating round cells, loosely attached round/oval or elongated cells with prominent pseudopod-like processes, to well-attached spindle and elongated dendritic cells without obvious pseudopods. (tmu.edu.tw)
  • This effect was tumor cell-specific and dependent on CD8(+) T cells evidenced by in vitro CD8(+) T cell depletion. (maastrichtuniversity.nl)
  • Not surprisingly, the regulation of this vital safeguard has been studied in great detail for many years but mainly in tissue culture, or in vitro, models. (news-medical.net)
  • Células cultivadas in vitro procedentes de tejido neoplásico que se utilizan en el estudio de las capacidades metabólicas y proliferativas de las células tumorales, en el pronóstico de las respuestas clínicas a la quimioterapia, en la detección de nuevos agentes antitumorales y en las investigaciones biológicas básicas. (bvsalud.org)
  • Cells grown in vitro from neoplastic tissue. (bvsalud.org)
  • This study supports ERV-K env as a single prognostic indicator for better survival of RCC, which we propose represents a new tumor antigen. (frontiersin.org)
  • T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)
  • Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
  • Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
  • We also propose this assay as an ex vivo platform for high-throughput screening of immunomodulating agents to be tested in these two murine tumor models. (maastrichtuniversity.nl)
  • Moreover, we recently reported that lycopene inhibits the metastasis of human liver adenocarcinoma SK-Hep-1 cells by downregulation of the NADPH oxidase 4 (NOX4) proteins ( 18 ). (frontiersin.org)
  • As of early 2016, there are three families belonging to the MACPF superfamily: 1.C.12 - The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family 1.C.39 - The Membrane Attack Complex/Perforin (MACPF) Family 1.C.97 - The Pleurotolysin Pore-forming (Pleurotolysin) Family Proteins containing MACPF domains play key roles in vertebrate immunity, embryonic development, and neural-cell migration. (wikipedia.org)
  • The MACPF domain is structurally similar to pore-forming cholesterol-dependent cytolysins from gram-positive bacteria, suggesting that MACPF proteins create pores and disrupt cell membranes similar to cytolysin. (wikipedia.org)
  • We postulated that the expression levels of angiogenesis-related proteins from various primary tumor cultures would be greater under hypoxic conditions than under normoxia. (biomedcentral.com)
  • When higher than normal levels of p53 tumor suppressor exist, there is enough p53 to bind to many regulatory sites in the cell's genome to activate the production of other proteins that will halt cell division if the DNA damage can be repaired. (news-medical.net)
  • We found that the three Id proteins expressed in neuroblastoma cells (Id1, Id2, and Id3) were down-regulated during induced differentiation, indicating that Id proteins help keep the tumor cells in an undifferentiated state. (lu.se)
  • The ability of Id proteins to affect HES-1 activity is of particular interest in neuronal cells, where regulation of HES-1 is essential for the timing of neuronal differentiation. (lu.se)
  • In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)
  • The epidermal growth factor receptor family (ERBB) is overexpressed in a wide variety of tumor types and is correlated with poor prognosis. (aacrjournals.org)
  • Melanoma tumour cells exhibit pronounced heterogeneity within a single tumour, amongst concurrent tumours within the same patient and amongst tumours from different patients. (nature.com)
  • The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)
  • We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote p.S668T mutation in nine of 42 samples and four of nine also had the p.D674H mutation. (scielo.br)
  • On the basis of these preclinical data, the ADC SAR408701 is a promising candidate for development as a potential treatment for patients with CEACAM5-positive tumors. (nih.gov)
  • The RNA-seq dataset contains raw sequencing data (fastq files) from the TSC pellets at the time of surgical resection, and the pellets of derived WTC cultures with or without tamoxifen metabolites treatment (1 nM 4OHT and 25 nM Z-Endoxifen), from 16 BC patients. (scilifelab.se)
  • The cell viability assay dataset for the main study contains drug sensitivity score (DSS) values for each of the tested drugs derived from the WTC spheroids of 45 BC patients. (scilifelab.se)
  • Exploring Tumor-Immune Interactions in Co-Culture Models of T Cells and Tumor Organoids Derived from Patients. (bvsalud.org)
  • To validate the results, they used blood cells from patients with a very rare congenital inflammatory disease in which RNaseT2 cannot be produced due to a genetic defect, and who suffer from severe mental and physical disability as a result. (uni-bonn.de)
  • The primary immune cells from these patients enabled the Bonn researchers to validate the results from the CRISPR-Cas9 model cell lines very well," says Prof. Dr. Jutta Gärtner, Director of the Department of Pediatrics and Adolescent Medicine at the University Medical Center Göttingen, who first described this disease and provided the Bonn researchers with immune cells from these rare patients. (uni-bonn.de)
  • With the development of tumor molecular heterogeneity theory(12), there are still some patients with GIST who are sensitive to radiotherapy, especially for the patients with advanced stage(13-16).In addition, with the development of imaging technology and modern tissue and organ radiation technology, it has become a reality to concentrate high-dose radiation locally in abdominal cavity tumors(12), which challenges RT's insensitivity to GISTs. (researchsquare.com)
  • Results from a clinical trial suggest that 177 Lu-Dotatate may soon be a new treatment option for some patients with advanced neuroendocrine tumors. (cancer.gov)
  • Pleural fluid analysis biopsy and lavage has increased the diag- was performed for protein concentration, nostic rate, the cause for many patients with lactic dehydrogenase (LDH), cultures, as exudative PE remains unknown or obscure. (who.int)
  • We recommend meloxicam for patients with extraabdominal desmoid tumors, instead of surgery first," said lead author Yoshihiro Nishida, MD, PhD, from the Department of Orthopedic Surgery at Nagoya University Graduate School of Medicine in Japan. (medscape.com)
  • In this study, between 1991 and 2003, Dr. Nishida and colleagues identified 30 patients who were surgically treated for extraabdominal desmoid tumors at their institutions (excluding those with intraabdominal, familial adenomatous polyposis-associated, and unresectable tumors). (medscape.com)
  • However, the significant morbidity of aggressive chemotherapy should be avoided in patients with extraabdominal desmoids tumors," they say. (medscape.com)
  • Glioblastoma multiforme (GBM) the highest-grade glioma and deadliest brain tumor occurs in pediatric as well as adult patients. (lu.se)
  • A complete cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts. (promocell.com)
  • January 5, 2010 - Meloxicam ( Mobic ), a cyclooxygenase (COX)-2 inhibitor drug used for arthritis, has shown promise as a treatment for extraabdominal desmoid tumors, according to the results of a small pilot study. (medscape.com)
  • Extraabdominal desmoid tumors are generally sporadic in nature and can occur in virtually any body site, note the authors. (medscape.com)
  • Baseline imaging of desmoid tumors by magnetic resonance imaging (MRI) was obtained prior to beginning treatment with oral meloxicam 10 mg/day. (medscape.com)
  • Surgery remains the mainstay for resectable sporadic desmoid tumors, especially extraabdominal ones, the authors write, and there is growing evidence that chemotherapy might be effective in managing life-threatening intraabdominal desmoid tumors. (medscape.com)
  • The effects of the two macrolides on tumor necrosis factor-α (TNF-α) release were also examined. (karger.com)
  • Cigana C, Assael BM, Melotti P: Azithromycin selectively reduces tumor necrosis factor alpha levels in cystic fibrosis airway epithelial cells. (karger.com)
  • Circulating tumour DNA (ctDNA) from the same patient blood sample, was assessed by ddPCR targeting tumour-specific mutations. (nature.com)
  • 2017. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. . (oregonstate.edu)
  • The cellular cascade of molecular signals that instructs cells with fatally damaged DNA to self-destruct pivots on the p53 tumor suppressor gene. (news-medical.net)
  • The advent of genomic sequencing technologies has enabled the comprehensive analysis of tumor characteristics. (bvsalud.org)
  • Specifically, ERV-K members were originally identified due to their similarity to the Mouse Mammary Tumor Virus (MMTV) and are subdivided into ten so-called human MMTV-like clades (HML1-10) ( 2 , 3 ). (frontiersin.org)
  • Ma Z, Gibson SL, Byrne MA, Zhang J, White MF and Shaw LM (2006) Suppression of insulin receptor substrate 1 (IRS-1) promotes mammary tumor metastasis. (scielo.br)
  • Mammary fat pad will be identified and breast carcinoma cells will be injected at the concentration of 1-5X10 6 in 30μL PBS or with 50% matrigel. (stanford.edu)
  • Although epigenetically silenced, endogenous retroviral (ERV) genes become activated in tumors and function to ignite immune responses. (frontiersin.org)
  • In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
  • Kim YD, Cho JS, Kim HS, Song SH: Effect of macrolide on the interleukin-1 β-mediated MUC2/5AC genes expression and mucin secretion in human airway epithelial cells. (karger.com)
  • Cells will be labeled with luciferase or other reporter genes. (stanford.edu)
  • Clinical studies using ERBB inhibitors have focused on tumor growth effects, but ERBBs can contribute to malignancy independent of their effects on tumor growth. (aacrjournals.org)
  • Further details on the cell viability assay, as well as the DSS estimation are available in the Materials & Methods part of Chen et al. (scilifelab.se)
  • RIP assay was performed to validate NGF and miR-21 binding ability in MiaPaCa-2 cells. (hindawi.com)
  • In this study, we developed a simple co-culture assay, reproducible in any lab, but respecting the multicellular nature of the TME. (maastrichtuniversity.nl)
  • Our goal is to combine in a single assay well-established techniques such as a luciferase assay for target cell viability analysis, a CD107a degranulation assay, and multicolor flow cytometry for the detection of cytokines and cytotoxicity markers. (maastrichtuniversity.nl)
  • Our co-culture assay is therefore suitable as proof of principle for in vivo therapeutic studies testing immunotherapies, and specifically to assess the involvement of cytotoxic CD8(+) T cells in treatment response in LLC and CT26 tumor models. (maastrichtuniversity.nl)
  • This assay can be adapted to other tumor models after optimizations. (maastrichtuniversity.nl)
  • Co-immunoprecipitation assay was applied for detecting the endogenous interaction of MAGEC2 and TRIM28 in tumor cells. (biomedcentral.com)
  • Total mucin in culture supernatants was measured using enzyme-linked lectin assay. (karger.com)
  • Enzyme-linked immunosorbent assay was used to determine MUC5AC, MUC2 and TNF-α released by the cells. (karger.com)
  • Immunostaining revealed that major melanoma-associated antigens, such as S100 protein, HMB-45, melanotransferrin, chondroitin sulphate proteoglycan, and the gangliosides GD2 and GD3, were consistently expressed by the tumour tissue, severe combined immunodeficiency (SCID) mouse xenograft and derived cell lines. (tmu.edu.tw)
  • This observation and the expression of the major melanoma-associated antigens were all indicative of the melanocytic origin of MST-1 tumour. (tmu.edu.tw)
  • Cell surface antigens are large molecules sticking out from a cell that help the immune system determine whether the cell is harmless or harmful. (stanford.edu)
  • Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
  • Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)
  • Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)
  • The therapeutic efficacy of immune checkpoint inhibitors has underscored the importance of immune cells , particularly cytotoxic T cells that infiltrate the vicinity of tumors , in patient prognosis . (bvsalud.org)
  • Analysis of lung tissue RNA using Ingenuity Pathway Analysis (IPA) indicated trends in inflammatory response and connective tissue organization markers, with an emphasis on immune cell trafficking, phagocytosis, and adaptive immune responses. (cdc.gov)
  • The cell enters a state of emergency, places itself in a kind of quarantine and produces inflammatory messengers that attract and activate other immune cells. (uni-bonn.de)
  • These immune cells can then kill infected cells or form antibodies against the pathogens and thus, ideally, fight off the infection in the long term. (uni-bonn.de)
  • Like a radar system, the immune sensor with the scientific name Toll-like Receptor 8 or "TLR8" monitors whether tell-tale ribonucleic acids (RNA) appear during the recycling of dead cells or ingestion of live pathogens, indicating foreign invaders. (uni-bonn.de)
  • As the research group leader at the Institute for Clinical Chemistry and Clinical Pharmacology at the University Hospital Bonn explains, it was only the advent of the CRISPR-Cas9 gene editing that made it possible to understand the importance of the TLR8 immune sensor in human cells. (uni-bonn.de)
  • The interaction of RNaseT2 and RNase2 with the immune sensor TLR8 is a key element of the immune response against pathogens inside cells," says Bartok. (uni-bonn.de)
  • Engineered human immune cells can vanquish a deadly pediatric brain tumor in a mouse model, a study from the Stanford University School of Medicine has demonstrated. (stanford.edu)
  • In addition, the ability of radiotherapy to stimulate both innate and adaptive immune cells' function has been shown to enhance the therapeutic benefit of immunotherapy in some cases. (unil.ch)
  • Researchers at IRB Barcelona report that the induction of senescence in tumour cells strongly stimulates the immune system. (irbbarcelona.org)
  • However, these same cells create an environment that blocks immune cells and protects the tumour. (irbbarcelona.org)
  • Due to the limited availability of current esophageal squamous cell carcinoma (ESCC) models, we aimed to establish a panel of ESCC patient-derived organoid (PDO) long-term cultures for ESCC study. (hku.hk)
  • Its function is implicated in a receptor tyrosine kinase signaling pathway that specifies differentiation and terminal cell fate. (wikipedia.org)
  • When tested in normal ovarian surface epithelial and ovarian clear cell carcinoma cell culture models, encapsulation of TPPS in cESG significantly improved cell death in response to light treatment compared to free drug alone. (nih.gov)
  • For VEGF, the target of current therapies, this correlation between hypoxia and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell lines. (biomedcentral.com)
  • however, mechanisms through which these bioactive compounds act on epithelial cells have not been identified. (biorxiv.org)
  • 16-membered) on MUC5AC and MUC2 gene expression and secretion from human airway epithelial cells. (karger.com)
  • Confluent NCI-H292 human mucoepidermoid airways epithelial cells were pretreated with AZM or MDM for 2 h and then stimulated with 200 nmol/l phorbol 12-myristate 13-acetate (PMA) for 8 h. (karger.com)
  • The present study demonstrates that AZM and MDM suppress the synthesis of mucin and TNF-α from human airway epithelial cells. (karger.com)
  • Shimizu T, Shimizu S: Azithromycin inhibits mucus hypersecretion from airway epithelial cells. (karger.com)
  • Moreover, Wnt-5a is known to inhibit migration of breast epithelial cells in culture and expression of Wnt-5a potentiates activation of the receptor tyrosine kinase DDR1, a collagen receptor implicated in cell adhesion and migration. (lu.se)
  • These results suggest that DARPP-32 restricts the migration of breast epithelial cells via both a transcription dependent mechanism that involves CREB and a transcription independent mechanism affecting FAK. (lu.se)
  • Approaches to characterize tumor development, progression, and metastasis in these models using state-of-the-art imaging, histopathological, surgical, and other techniques are also included. (cshlpress.com)
  • Human cell cultures: glioblastoma (U87), colorectal human adenocarcinoma (SW-620), human melanoma (SK-Mel28) and primary embryonic cell lines were irradiated with a neutron flux at the presence of BPA, BSH and liposomal BSH with a concentration of10B 40 μg/ml. (isnct.net)
  • The data obtained indicate that the accelerator-based BNCT using boron delivery drugs, such as borphenylalanine, borcaptate and liposomal borcaptat, has a positive effect on tumor lines of glioblastoma, colorectal adenocarcinoma and melanoma. (isnct.net)
  • Trim24, important for TH2 cell effector function, was downregulated in both datasets. (cdc.gov)
  • In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
  • In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)
  • Circulating tumour cells (CTCs), which are responsible for haematogenous spread of tumours, also reflect this heterogeneity. (nature.com)
  • Exposure to acetaldehyde has produced nasal tumors in rats and laryngeal tumors in hamsters, and exposure to malonaldehyde has produced thyroid gland and pancreatic islet cell tumors in rats. (cdc.gov)
  • For the successful implementation of BNCT, boron delivery drugs that must be selectively accumulated in malignant cells in a sufficient amount, and a neutron source with the energy required for the neutron capture reaction are needed. (isnct.net)
  • In an abnormal result, there are cancerous (malignant) cells. (medlineplus.gov)
  • Morbidity rates for some fl ocks ulated intraabdominally with a 1,000- ence and Technology Development (no. reached 60%, and mortality rates for unit 50% tissue-culture infective dose 2010AA6AN034). (cdc.gov)
  • Previous tissue culture studies by several labs around the world indicated that tinkering with the tail end prevented the protein from being flagged for degradation or activation. (news-medical.net)
  • The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
  • The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
  • Our cells are vulnerable to DNA breaks caused by UV light, ionizing radiation, toxic chemicals or other environmental damages. (news-medical.net)
  • Next, using a lung adenocarcinoma model (KP), we demonstrated that modulation of neutrophil phenotype was able to enhance the tumor response to radiation therapy without any stimulation of fibrosis. (unil.ch)
  • Despite the crucial role of radiation resistance in patient mortality, few viable therapeutic strategies have been identified to target these resistant cells. (lu.se)
  • Infiltration versus metastasis is one of the most characteristic biological properties of tumors [ 5 ]. (hindawi.com)
  • Synthesis and biological evaluation of new 3(2)-pyridazinone derivatives as non-toxic anti-proliferative compounds against human colon carcinoma HCT116 cells. (amrita.edu)
  • This has led to investigations of both cytotoxic and noncytotoxic chemotherapy, but because the tumors are rarely fatal, finding a pharmacologic treatment with fewer complications is desirable, according to the authors. (medscape.com)
  • Despite aggressive treatment with surgery, irradiation and sometimes chemotherapy, tumors invariably recur as incurable lesions. (lu.se)
  • More recently, tumour-treating fields therapy (TTFields) has demonstrated modest survival benefit and been clinically approved in several countries. (nature.com)
  • Tumour treating fields (TTFields) therapy is a non-invasive therapy which delivers low-intensity (1-3 V/cm) intermediate-frequency (100-500 kHz) alternating electric fields to localised tumour sites [ 29 ]. (nature.com)
  • Essential oil prepared by distillation of the gum resin traditionally used for aromatic therapy has also been shown to have tumor cell-specific anti-proliferative and pro-apoptotic activities. (biomedcentral.com)
  • These tumor organoids have emerged as innovative tools that are extensively employed in drug testing, genome editing , and transplantation to guide personalized therapy in clinical settings. (bvsalud.org)
  • One CAR T-Cell Therapy for Blood Cancers? (cancer.gov)
  • Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
  • One such idea is cell replacement therapy (CRT). (lu.se)
  • Firstly, like C9, high concentrations of perforin can form pores that lyse cells. (wikipedia.org)
  • Interleukin-2 (IL-2) expanded TILs had the predominant CD8 + phenotype and the capacity to lyse cells of the cultured autologous tumour. (tmu.edu.tw)
  • Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)
  • In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. (frontiersin.org)
  • Characteristics of cultured desmoid cells with different CTNNB1 mutation status. (dtrf.org)
  • Excess expression of the sugar is caused by the same mutation, known as the H3K27M mutation, that drives the growth of most DIPG tumors, the team found. (stanford.edu)
  • In a dish, Mackall's CAR-T cells killed cultured DIPG cells that carry the H3K27M mutation. (stanford.edu)
  • Visit the Brain Tumor Biology groups research portal via this link. (lu.se)
  • Specific activation of the T population was induced by mixed lymphocyte tumor culture in chambers implanted to rats. (ui.ac.id)
  • We demonstrate that DARPP-32 inhibits MCF-7 cell migration and that this effect requires phosphorylation of Threonine-34, an event catalyzed by protein kinase A (PKA) and strongly induced by detachment of cells from the culture substrate. (lu.se)
  • Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
  • High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. (frontiersin.org)
  • Cell survival 96 hours after irradiation was determined using MTT test, and survival fraction was evaluated using the clonogenic test. (isnct.net)
  • NGF promoted MiaPaCa-2 and CFPAC-1 cell invasion and migration, while Tanezumab treatment showed the opposite results. (hindawi.com)
  • TrkA blocking-up could restrain NGF induced PC cell migration and neural invasion. (hindawi.com)
  • In vivo tumorigenesis experiments, Tanezumab markedly alleviated nerve invasion of PC cells as well as relieved nociceptive conduction in animal models. (hindawi.com)
  • These experiments temporally and molecularly separate two key stages in tumor cell entry into blood vessels: invasion and intravasation. (aacrjournals.org)
  • The establishment and characterisation of paired autologous tumour cell line (MST-1) and tumour-infiltrating lymphocyte (TIL) culture from a tumour mass of a 14-year-old Taiwanese girl with soft tissue melanoma are described. (tmu.edu.tw)
  • Flow cytometric analysis of the tumour DNA content showed an index of 1.8 relative to normal peripheral blood lymphocyte DNA. (tmu.edu.tw)
  • Perspectives of tumor-infiltrating lymphocyte treatment in solid tumors. (msdmanuals.com)
  • Although antilymphocyte serum was used in the treatment of chronic lymphocytic leukemia and in T-cell and B-cell lymphomas, resulting in temporary decreases in lymphocyte counts or lymph node size, newer humoral immunotherapeutic modalities have been developed. (msdmanuals.com)