• Summing up, we showed that addition of L-arginine at doses which did not stimulate NO level caused a significant decrease in COX-2 and PGE 2 amounts in co-cultures of colon tumor spheroids with normal epithelial cells and myofibroblasts. (springer.com)
  • BACKGROUND: MCF-10A cells are near diploid and normal human mammary epithelial cells. (biomedsearch.com)
  • Putative progenitor cell populations were isolated based on co-expression or concomitant absence of the epithelial and myoepithelial markers EPCAM and CALLA respectively. (lenus.ie)
  • Epithelial cell adhesion molecule (EpCAM) is an epithelial cell-specific adhesion molecule that is widely expressed on the surface of epithelial cells and epithelial-derived tumor cells ( 6 ). (aacrjournals.org)
  • Although HCC cells are epithelial cells, only about 35% of HCC cases express EpCAM ( 8, 9 ). (aacrjournals.org)
  • Aberrant Notch signaling was implicated in the initiation of T-cell lymphoblastic leukemia in the early 1990s ( 7 ), and has since been demonstrated in many different hematopoietic and epithelial tumors ( 8-10 ). (aacrjournals.org)
  • In the presence of chronic liver damage, we show that ablation of a p53-dependent senescence program in hepatic stellate cells increases liver fibrosis and cirrhosis associated with reduced survival, and enhances the transformation of adjacent epithelial cells into hepatocellular carcinoma. (pubmedcentralcanada.ca)
  • We now demonstrate that HGF induced the trafficking of lysosomes to the cell periphery, independent of HGF-induced epithelial-mesenchymal transition. (biologists.org)
  • This protective effect was associated with increased stress fiber formation, cell-matrix, and cell-cell adhesion in the shRNARelB (shRelB) cells during cisplatin/TNF- α treatment, mimicking an epithelial-to-mesenchymal phenotypic switch. (aspetjournals.org)
  • The proximal tubular epithelial cells (PTECs) are the primary target. (aspetjournals.org)
  • In contrast to so far described exclusive expression of Robo 4 in the tumor vascular network, our analyses showed that in PCa Robo 4 is not only expressed in the tumor stroma but also in cancer epithelial cells. (medsci.org)
  • In summary we observed that Robo 4 plays a considerable role in PCa development as it is expressed in cancer epithelial cells as well as in the surrounding tumor stroma. (medsci.org)
  • Since then, various other phenotypic characteristics have been associated with breast CSCs including high aldehyde dehydrogenase (ALDH) activity ( 4 ), an enhanced capacity to generate mammospheres, a surrogate marker for self-renewal and stem cells ( 5, 6 ), and activation of an epithelial-mesenchymal transition (EMT) program ( 7, 8 ). (aacrjournals.org)
  • Isolated tumor endothelial cells maintain specific character during long-term culture. (nih.gov)
  • Such a marked suppression by TNF-α was not observed in confluent bovine aortic endothelial cells and human fibroblastic IMR-90 cells. (elsevier.com)
  • Increasing evidence indicates that tumor endothelial cells (TEC) differ from normal endothelial cells (NEC). (plos.org)
  • Furthermore, there are many differences at the molecular and functional levels between tumor endothelial cells (TEC) and normal EC (NEC) , . (plos.org)
  • Compared with control condition, pretreatment of carvedilol (10 μmol/L for 18 hours) or probucol (5 μmol/L for 18 hours), but not propanolol, prazosin, or both propanolol and prazosin significantly decreased TNF-α-stimulated adhesiveness of cultured human aortic endothelial cells (HAECs) to MNCs. (ahajournals.org)
  • In the early phase of atherogenesis, cell surface adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intercellular cell adhesion molecule-1 (ICAM-1), and endothelial cell selectin (E-selectin) could express on endothelial cells to recruit circulating mononuclear cells (MNCs), mainly monocytes, and facilitate their binding to endothelium and migrating to subendothelial space. (ahajournals.org)
  • 1-3 Reactive oxygen species (ROS) may serve as a common intracellular messenger for various redox-sensitive transcription pathways that lead to adhesion molecule expression in vascular endothelial cells. (ahajournals.org)
  • The pseudocysts are surrounded by tumor cells and are filled with extracellular matrix components such as basement membrane molecules. (nii.ac.jp)
  • Although in tumor cells, curcumin did not significantly affect IR-induced activation of AKT and nuclear factor-κB, we found that it caused a significant increase in the production of reactive oxygen species, which further led to sustained extracellular signal-regulated kinase (ERK) 1/2 activation. (aspetjournals.org)
  • It was previously shown that acidic extracellular pH stimulated peripheral lysosome trafficking, resulting in increased cathepsin B secretion and tumor cell invasion, which was dependent upon sodium-proton exchanger (NHE) activity. (biologists.org)
  • Solid tumors are also protected by an extracellular matrix, a supportive web of proteins that acts as a barrier, as well as immunosuppressive molecules that weaken the T-cell attack. (phys.org)
  • The Hynes team targeted the nanobodies to the tumors' extracellular matrix , or ECM-aiming imaging agents not at the cancer cells themselves, but at the environment that surrounds them. (phys.org)
  • We hypothesized that BLBC cells are able to establish a niche permissive to the maintenance of CSCs and found that tumor cell-derived periostin (POSTN), a component of the extracellular matrix, as well as a corresponding cognate receptor, integrin α v β 3 , are highly expressed in a subset of BLBC cell lines as well as in CSC-enriched populations. (aacrjournals.org)
  • These clonal lines may provide a suitable model system for the study of gonadotropin actions and regulation of the expression of differentiated functions of Leydig cells. (nih.gov)
  • However, it is unclear how these microenvironmental conditions interact to promote neovascularization, due in part to a lack of comprehensive, unbiased data sets describing tumor cell gene expression as a function of oxygen levels within three-dimensional (3D) culture. (sigmaaldrich.com)
  • Microarray gene expression analysis of tumor cells cultured in 2D versus 3D under ambient or hypoxic conditions revealed striking interdependence between culture dimensionality and hypoxia response, which was mediated in part by pro-inflammatory signaling pathways. (sigmaaldrich.com)
  • We report that cell surface PSA expression can be reversibly inhibited by a small molecule, N -butanoylmannosamine (ManBut). (sciencemag.org)
  • Cells are labeled green by GFP expression. (dsmz.de)
  • Cell lysis for Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. (scirp.org)
  • For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were considered to be of good quality. (scirp.org)
  • Proliferative Activity of Canine Mast Cell Tumours Evaluated by Bromodeoxyuridine Incorporation and Ki-67 Expression," Journal of Comparative Pathology, Vol. 127, No. 4, 2002, pp. 233-238. (scirp.org)
  • Xenografts generated from CTCs and parental cells were compared.Asparagine synthetase (ASNS) expression was also higher in CTCs.Xenografts generated from CTCs were larger and heavier, and metastasised faster than MDA-MB-231 xenografts. (nih.gov)
  • B) Expression of the anoxia factor ATF3 in CTCs and MDA-MB-231 cells. (nih.gov)
  • D) Expression of ATF4 and its target gene ASNS in CTCs, compared with parental MDA-MB-231 cells. (nih.gov)
  • RAD51 expression was observed broadly and was not associated with PTEN status in the 16 cell lines. (biomedcentral.com)
  • The expression level of nuclear PTEN was not elevated within 24 h following IR in the HEC-6-PTEN + cells. (biomedcentral.com)
  • Phenotypic characterization of the monocytes after 3D co-culture with tumor/fibroblast spheroids was performed by analysing the expression of defined cell surface markers and soluble factors. (uni-wuerzburg.de)
  • In particular, the let-7 miRNA family has been proposed to function in tumor suppression, because reduced expression of let-7 family members is common in non-small cell lung cancer (NSCLC). (pnas.org)
  • Ectopic expression of let-7g in K-Ras G12D -expressing murine lung cancer cells induced both cell cycle arrest and cell death. (pnas.org)
  • Ectopic expression of K-Ras G12D largely rescued let-7g mediated tumor suppression, whereas ectopic expression of HMGA2 was less effective. (pnas.org)
  • Finally, in an autochthonous model of NSCLC in the mouse, let-7g expression substantially reduced lung tumor burden. (pnas.org)
  • DMP1 binds to a single canonical recognition site in the ARF promoter to activate gene expression, and in turn, p19 ARF synthesis causes p53-dependent cell cycle arrest. (pnas.org)
  • Using serial gene expression analysis, we report functional evidence of vaccine-induced CTL reactivity in fresh cells obtained directly from the peripheral blood of postimmunized patients. (jimmunol.org)
  • and assessing tumor tropic potential of the stem cell based upon said expression level of CXCR4, said affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
  • Limiting PD-L1 expression and function is therefore critical for allowing the development of antitumor immune responses and effective tumor clearance. (frontiersin.org)
  • Here, we provide the first evidence of a role for PKM2 in regulating the expression of PD-L1 on macrophages, dendritic cells (DCs), T cells, and tumor cells. (frontiersin.org)
  • Moreover, TEPP-46 inhibited expression of PD-L1 on macrophages, DCs, and T cells as well as tumor cells in a mouse CT26 cancer model. (frontiersin.org)
  • Following the down-expression of TET1, the level of autophagy in U251 cells decreased accordingly.However, when TET1 was overexpressed in U251 cells, the level of autophagy incraesed. (portlandpress.com)
  • RND3 expression was found to be significantly lower in CRC tissues than in normal tissues and adenomas, indicating that RND3 may act as a tumour suppressor gene in CRC. (portlandpress.com)
  • NK cells have organ-specific properties, such as distinct profiles of receptor expression or cytokine production ( 3 ). (aacrjournals.org)
  • Aberrant expression of the oncogenic transcription factor forkhead box protein 1 (FOXP1) is a common feature of diffuse large B-cell lymphoma (DLBCL). (bloodjournal.org)
  • 7 Therefore, aberrant FOXP1 expression is a common feature of various types of mature B-cell lymphomas that can result from either genetic abnormalities or transcriptional or posttranscriptional dysregulation. (bloodjournal.org)
  • however, aberrant expression is associated with tumor initiation in a wide variety of human cancers, including breast cancer and leukemia. (biologists.org)
  • The expression of miR-218 was negatively correlated with IDO1 in cervical cancer tissues and cells. (springer.com)
  • Conversely, high expression of EGFR ligands predicts response to anti-EGFR therapy but only in the subset of wild-type KRAS tumors [ 15,16 ]. (pubmedcentralcanada.ca)
  • In line with other reports, GEM and OXP upregulated expression of the death receptor CD95 (fas) on live cells even at sub-cytotoxic concentrations. (sgul.ac.uk)
  • In advanced stage we observed a hypothesis-generating trend that high Robo 4 and Slit 2 expression is associated with delayed development of tumor recurrence compared to patients with low Robo 4 and Slit 2 expression, respectively. (medsci.org)
  • We found that in 21 out of 28 comparisons, the presence or absence of detectable gene expression in CTCs and PCa cells microdissected from single bone lesions of the same patients was concordant. (biomedcentral.com)
  • Characterization of several clonal lines of cultured Leydig tumor cells: gonadotropin receptors and steroidogenic responses. (nih.gov)
  • The goal of this study was to investigate activation of the endoplasmic reticulum (ER) stress by jasmonic acid (JA) and to distinguish between the responses of cultured immortalized non-tumorigenic HaCaT cells and epidermoid carcinoma A431 cells to this plant hormone. (springer.com)
  • Oncolytic HSV-1 therapy is dependent upon virus replication in tumor cells and is augmented by host antiviral and infection-induced antitumor immune responses ( 1 , 3 - 6 ). (asm.org)
  • Here we have characterized tumour cell responses to two new inhibitors of FGFR1-3, AZ12908010 and the clinical candidate AZD4547, making comparisons with the well-characterized FGFR inhibitor PD173074. (nature.com)
  • Autophagosomes derived from tumor cells, also referred to as defective ribosomal products in blebs (DRibbles), have been previously shown to stimulate potent T-cell responses and mediate tumor regression when used as therapeutic cancer vaccines in multiple preclinical cancer models. (nature.com)
  • Our data show that DRibbles could induce strong innate immune responses via multiple pattern recognition receptors, and explain why DRibbles could function as excellent antigen carriers to induce adaptive immune responses to both tumor cells and viruses. (nature.com)
  • For multicellular organisms, autophagy serves an important role as a cell-intrinsic defense mechanism and a key regulator of inflammatory responses. (nature.com)
  • Hypoxia-inducible transcription factors (HIFs) regulate a wide array of adaptive responses to hypoxia and are often activated in solid tumors and hematologic malignancies due to intratumoral hypoxia and emerging new layers of regulation. (bloodjournal.org)
  • Perhaps the strongest implication for a role for NKT cells in Th1 immunity is in tumor rejection responses after exogenous IL-12 ( 9 )( 28 )( 29 )( 30 ) or α-GalCer ( 11 )( 31 ) administration. (rupress.org)
  • The inability to control tumor outgrowth can be due to inadequate T helper responses underlying poor tumor-specific immunity. (rupress.org)
  • The research scientist in charge of manufacturing the CAR-T cell product and following the patients' responses to treatment is UniSA's Dr Tessa Gargett, a Cancer Council Beat Cancer Project Early Career Fellow from the Centre for Cancer Biology. (news-medical.net)
  • Our findings provide mechanistic evidence that PVSRIPO functions as a potent intratumor immune adjuvant that generates tumor antigen-specific cytotoxic T lymphocyte responses. (sciencemag.org)
  • However, a limitation of immune checkpoint inhibitors, specifically those that target PD1, is their reliance on preexisting antitumor immune responses and the presence of CD8 T cells in the tumor ( 1 ). (sciencemag.org)
  • In SCT cultures, in-field responses showed no significant time dependency while out-of-field responses occurred early, and plateaued 6 h after irradiation in both DU-145 and H460 cells. (qub.ac.uk)
  • Under modulated beam configurations MCT co-cultures showed cell-specific, differential out-of-field responses depending on the irradiated in-field and responding out-of-field cell type. (qub.ac.uk)
  • The observed differential out-of-field responses may be due to the genetic background of the cells, in particular p53 status, which has been shown to mediate radiation-induced bystander effects (RIBEs). (qub.ac.uk)
  • INTRODUCTION: Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell precursors (angioblasts). (biomedsearch.com)
  • We have proposed that their frequent invasion of peripheral nerves, blood vessels, and skeletal muscles can be interpreted to mean that adenoid cystic carcinoma cells have an affinity for basement membranes. (nii.ac.jp)
  • Tumor blood vessels are different from their normal counterparts and have altered morphology, altered blood flow, enhanced leakiness, structural abnormalities in the basement membrane, and abnormal pericytes . (plos.org)
  • One protein, EIIIB, a variant of fibronectin, is found only on newly formed blood vessels that supply tumors with nutrients. (phys.org)
  • Targeting EIIIB may damage blood vessels in a way that decreases blood supply to tumors, while making them more permeable to cancer drugs. (phys.org)
  • Briefly, granular proteins were differentially extracted from GRC.014.22 and GRC.014.24, eosinophilic cell lines established in our laboratory from a patient with moderate asthma. (scirp.org)
  • Basic proteins and polyamino acids are taken up by mammalian cells at rates up to 3000 times greater than serum albumin. (sciencemag.org)
  • Hypoxia induced lower levels of carbonic anhydrase IX (CAIX), GLUT1 and BCL2/adenovirus E1B 19-KD protein-interacting protein 3 (BNIP3) proteins in CTCs than in parental cells, supporting an altered hypoxia response. (nih.gov)
  • The infected human cells expressed, upon cultivation with dexamethasone, MMTV structural proteins and released spiked B-type virions, the infectivity of which could be neutralized by anti-MMTV antibody. (biomedcentral.com)
  • The cognate human and mouse proteins are 95% identical, and hDMP1 on human chromosome 7q21 is frequently deleted in myeloid leukemia, connoting a possible role for DMP1 as a tumor suppressor ( 28 ). (pnas.org)
  • Tumor-derived MVs (TMV) contain bioactive molecules such as microRNAs (miRNA), mRNAs, and/or proteins inside themselves . (plos.org)
  • A total of 28 differential proteins (12 up-regulated and 16 down-regulated) in CAL-27 cells treated with 11-dehydrosinulariolide have been identified by LC-MS/MS analysis. (mdpi.com)
  • It's been hard to find cancer-specific proteins on solid tumors that could serve as safe targets. (phys.org)
  • The CAR T cells they created were studded with nanobodies that recognize specific proteins in the tumor environment, bearing signals directing them to kill any cell they bound to. (phys.org)
  • As of early 2016, there are three families belonging to the MACPF superfamily: 1.C.12 - The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family 1.C.39 - The Membrane Attack Complex/Perforin (MACPF) Family 1.C.97 - The Pleurotolysin Pore-forming (Pleurotolysin) Family Proteins containing MACPF domains play key roles in vertebrate immunity, embryonic development, and neural-cell migration. (wikipedia.org)
  • The MACPF domain is structurally similar to pore-forming cholesterol-dependent cytolysins from gram-positive bacteria, suggesting that MACPF proteins create pores and disrupt cell membranes similar to cytolysin. (wikipedia.org)
  • In this study, we aimed to investigate the associations between COX-2, PGE 2 and NO in co-cultures of human colon cancer spheroids obtained from different tumor grades with normal human colonic epithelium and myofibroblast monolayers. (springer.com)
  • ABCG2 promoter luciferase reporter gene assay showing that the promoter region harboring the HIF-1α response element is required for the activation of ABCG2 in HCT‐116 human colon cancer cell line. (hindawi.com)
  • ABCG2 is an efflux transporter commonly found to overexpress in multidrug resistant (MDR) cancer cells. (hindawi.com)
  • Cancer cells do not exist in isolation. (hindawi.com)
  • Cancer cells are often confronted with a remarkable reduction in oxygen supply inside solid tumors, leading to intratumoral hypoxia. (hindawi.com)
  • The group of Dr. Sonja Eberth focuses on transcriptional deregulation in cancer and its functional impact on the biology of cancer cells, especially in variants of blood cancer, namely leukemia and lymphoma. (dsmz.de)
  • Vlad-Fiegen A, Freytag NV, Dorn S, Müller O, Eberth S. The Wnt pathway target gene CCND1 changes mitochondrial localization and decreases mitochondrial activity in colorectal cancer cell line SW480. (dsmz.de)
  • The presence of red blood cells (RBCs) hampers the accurate determination of cell viability as well as absolute cell counts for tumor-infiltrating leukocytes, cancer stem cells, and other tumor-resident cells. (miltenyibiotec.com)
  • AMSBIO has introduced a new range of cancer cell line models and culture media for the most difficult to culture tumor types where no models may exist. (technologynetworks.com)
  • Unlike traditional protocols for cell line creation, these cancer models eliminate the possibility of large scale cellular adaptation through culture and genetic drift. (technologynetworks.com)
  • Beneficially the cancer cell models are stable and show a high level of agreement to the original tumor genotype through 150 passages via SNP analysis. (technologynetworks.com)
  • The quality control of the cancer cell models includes screened for all major categories of cell contaminants, including mycoplasma, microbial contaminants, viruses, cell line cross-contamination and STR profiling. (technologynetworks.com)
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • We examined the anti-tumor activity of olaparib, a PARP inhibitor, and its correlation between the sensitivity and status of PTEN in endometrial cancer cell lines. (biomedcentral.com)
  • Our results suggest that olaparib, a PARP inhibitor, is effective on certain endometrial cancer cell lines. (biomedcentral.com)
  • IMPORTANCE Cancer cells are exposed to HSV-1 during oncolytic virotherapy with the intention of killing tumor cells. (asm.org)
  • Brain tumors are the leading cause of cancer-related death in children but high-grade gliomas in children and adolescents have remained a relatively under-investigated disease despite this. (semanticscholar.org)
  • All-trans retinoic acid modulates cancer stem cells of glioblastoma multiforme in an MAPK-dependent manner. (semanticscholar.org)
  • Pancreatic cancer (PC) remains one of the most challenging solid tumors to treat with a high unmet medical need as patients poorly respond to standard-of-care-therapies. (uni-wuerzburg.de)
  • Matrigel influences morphology and cathepsin B distribution of prostate cancer PC3 cells. (semanticscholar.org)
  • Importantly, let-7g-mediated tumor suppression was more potent in lung cancer cell lines harboring oncogenic K-Ras mutations than in lines with other mutations. (pnas.org)
  • The singularly most frequently disrupted gene in cancer is p53, whose loss of function occurs in more than half of human tumors ( 1 ). (pnas.org)
  • INK4a / ARF is perhaps the second most commonly disrupted locus in cancer cells ( 12 ). (pnas.org)
  • Not surprisingly, human cancer cells that retain p53 function overexpress Mdm2 ( 25 ) or sustain deletions that dismantle ARF function ( 12 ). (pnas.org)
  • ATCC Tumor Cell Panels combine authenticated, well-characterized cell lines with mutation data from the Sanger Institute Catalogue of Somatic Mutations in Cancer (COSMIC) to create powerful tools for cancer research and drug discovery. (atcc.org)
  • PD-L1 is a member of the B7 family of costimulatory/coinhibitory molecules and is expressed on a wide range of cell types, including cancer cells ( 3 , 5 ). (frontiersin.org)
  • T-cell therapies are not just for cancer. (the-scientist.com)
  • BIGHEART Acting Director Lim Chwee Teck and team have developed a unique approach for growing patient's cancer cells in a microfluidic device that enables doctors to test a range of drugs on tumour cell clusters to determine efficacy, and develop personalised therapies. (nus.edu.sg)
  • Because only the residual cancer cells outside the liver of a recipient can cause recurrence after liver transplantation in HCC patients, isolated tumor cells must be the source of recurrence ( 4 ). (aacrjournals.org)
  • MSCs are able to deliver drugs to target cells, and are an ideal candidate for cancer therapy. (medsci.org)
  • The phase 1 clinical trial will test the feasibility and safety of CAR-T cells - genetically modified white blood cells harvested from a patient's own blood with the unique ability to directly attack and kill cancers - to treat advanced solid tumors including small cell lung cancer, sarcomas and triple negative breast cancer. (news-medical.net)
  • Chimeric antigen receptor (CAR) T cells are a promising new technology in the field of cancer immunotherapy. (news-medical.net)
  • The research forms part of the 'CARPETS' phase 1 clinical trial initiated by Professor Michael Brown, Director of the Cancer Clinical Trials Unit at the RAH to treat advanced melanoma patients, now extended to include patients with other advanced solid tumors including, small cell lung cancer, sarcomas and triple negative breast cancer. (news-medical.net)
  • Oncolytic viruses, which are optimized to kill cancer cells without harming the surrounding normal tissues, have gathered increasing interest in recent years. (sciencemag.org)
  • Two of the many areas of scientific study where uses of cell culture techniques have had major impact are human aging and cancer research. (encyclopedia.com)
  • Cancer cell lines, which can grow indefinitely in culture, also retain long telomeres. (encyclopedia.com)
  • In the journal PNAS this week, researchers at Boston Children's Hospital and MIT show that these mini-antibodies, shrunk further to create so-called nanobodies, may help solve a problem in the cancer field: making CAR T-cell therapies work in solid tumors . (phys.org)
  • Highly promising for blood cancers , chimeric antigen receptor (CAR) T-cell therapy genetically engineers a patient's own T cells to make them better at attacking cancer cells. (phys.org)
  • The Dana-Farber/Boston Children's Cancer and Blood Disorders Center is currently using CAR T-cell therapy for relapsed acute lymphocytic leukemia (ALL), for example. (phys.org)
  • Targeted cancer therapy is based on exploiting selective dependencies of tumor cells. (elifesciences.org)
  • We demonstrate that WRN inactivation selectively impairs the viability of MSI-H but not microsatellite stable (MSS) colorectal and endometrial cancer cell lines. (elifesciences.org)
  • we identify WRN helicase as a selective dependency in MSI-H cancer cell lines. (elifesciences.org)
  • Department of Molecular and Cell Biology and Cancer Research Laboratory, Division of Immunology, University of California at Berkeley, Berkeley, California. (aacrjournals.org)
  • Low numbers of PC3 prostate cancer (PCa) cells were spiked into normal blood to assess cell recovery rate. (biomedcentral.com)
  • CTCs are rare cancer cells transported through the peripheral circulation. (biomedcentral.com)
  • Basal-like breast cancer (BLBC) is an aggressive subtype of breast cancer which is often enriched with cancer stem cells (CSC), but the underlying molecular basis for this connection remains elusive. (aacrjournals.org)
  • Following vaccination of breast cancer patients with the HER2 protein vaccine, HER2-specific antibodies were detectable and these antibodies bound to cell surface-expressed HER2 and inhibited HER2 signaling through blocking tyrosine 877 phosphorylation of HER2. (biomedcentral.com)
  • Although freshly isolated cells derived from the M5480P tumor have functional hCG receptors, only two of the five clonal lines established were shown to bind significant quantities of hCG. (nih.gov)
  • We demonstrate the parallel production of one hundred synthetic 3D solid tumors comprising up to thousands of human hepatocellular carcinoma (HCC) HepG2 cells, we characterize the tumor structure by high-resolution optical microscopy, and we monitor the functional behavior of natural killer (NK) cells migrating, docking and interacting with the tumor model during culture. (diva-portal.org)
  • It provides a feasible mechanism of early tumour vascular supply which can co-exist and incorporate with later angiogenic mechanisms. (biomedsearch.com)
  • Importantly, glucose deprivation and hypoxia were also found to enhance the resistance level of ABCG2-overexpressing resistant cells with pre-existing genetic and epigenetic MDR mechanisms. (hindawi.com)
  • The success of FGFR inhibitors (FGFRi) will require knowledge of their cellular selectivity, determinants of intrinsic tumour cell sensitivity/resistance and the mechanisms by which tumour cells adapt (acquired resistance). (nature.com)
  • In this report, we investigated the underlining mechanisms by which DRibbles induced T-cell activation, particularly how DRibbles activated antigen-presenting cells (APCs). (nature.com)
  • The main effector cells responsible for tumor eradication were identified as CD8 + cytotoxic T cells that were found to recognize a recently described immunodominant viral gag-encoded cytotoxic T lymphocyte (CTL) epitope, which is unrelated to the viral env-encoded T helper peptide sequence. (rupress.org)
  • 15. The formulation of claim 13, wherein said tumor cells are selected from the group consisting of melanoma cells, lung carcinoma cells, sarcomas, prostate carcinoma cells, breast carcinoma cells, colon carcinoma cells and cervical carcinoma cells. (freepatentsonline.com)
  • To better understand the interaction of HSV-1 with neoplastic cells, we inoculated three-dimensional (3D) cultures of human uveal melanoma cells with HSV-1. (asm.org)
  • When established 3D melanoma cultures were inoculated with HSV-1 by placing virus on the surface of cultures, virus infection caused extensive death of melanoma cells growing on the surface of the 3D matrix and significantly decreased the number of tumor cell spheroids within the matrix. (asm.org)
  • The recent identification and cloning of melanoma-associated Ags recognized by T cells ( 2 , 3 ) have provided potentially new tools to enhance natural immunity by the same approach that has been successful in the immunization against foreign pathogens. (jimmunol.org)
  • Similar results were observed in a melanoma-derived cell line. (aacrjournals.org)
  • It has been reported that TET1 is a tumour suppressor in several cancers. (portlandpress.com)
  • MicroRNA-218 (miR-218) is a tumor-suppressive miRNA in cancers. (springer.com)
  • These 'nanobodies' could help highly promising CAR T-cell therapies kill solid tumors, where right now they work only in blood cancers. (phys.org)
  • Another, PD-L1, is an immunosuppressive protein that most cancers use to silence approaching T cells. (phys.org)
  • Sustained HER2 signaling at the cell surface is an oncogenic mechanism in a significant proportion of breast cancers. (biomedcentral.com)
  • The effects of PTEN on the sensitivity to olaparib and ionizing radiation (IR) exposure were compared between parental HEC-6 (PTEN-null) and HEC-6 PTEN + (stably expressing wild-type PTEN) cells by clonogenic assay, foci formation of RAD51 and γH2AX, and induction of cleaved PARP. (biomedcentral.com)
  • Interlaboratory studies with the chinese hamster v79 cell metabolic cooperation assay to detect tumor-promoting agents. (cdc.gov)
  • The authors conclude that several characteristics of the assay may limit its usefulness for screening broad classes of chemicals for potential activity as tumor promoters. (cdc.gov)
  • in the same cells, AA promotes NO release even in the absence of calcium entry, giving rise to a complex signaling pathway for NSOCE regulation ( 6 ). (aacrjournals.org)
  • The ability to effectively culture CTCs and examine them for genetic changes can be used to find targetable mutations and discover which therapeutic drugs are most effective. (oncologynurseadvisor.com)
  • In a panel of 16 human tumour cell lines, the anti-proliferative activity of AZ12908010 or AZD4547 was strongly linked to the presence of deregulated FGFR signalling, indicating that addiction to deregulated FGFRs provides a therapeutic opportunity for selective intervention. (nature.com)
  • The present invention relates to tumor tropic stem cells, and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. (freepatentsonline.com)
  • 6. The isolated stem cell of claim 5, wherein said heterologous gene encodes a polypeptide of therapeutic use in the treatment of a disease condition. (freepatentsonline.com)
  • Mesenchymal stem cells (MSCs) show therapeutic effects in various types of diseases. (medsci.org)
  • they may also serve as a potential type of therapeutic cells. (medsci.org)
  • The emergence of therapeutic resistance is a significant concern when targeting many signaling pathways and tumor types. (aacrjournals.org)
  • Here, we utilized alginate-based, oxygen-controlled 3D tumor models to study the interdependence of culture context and the hypoxia response. (sigmaaldrich.com)
  • Reporter activity in HCT-116 cells transiently transfected with the various ABCG2 promoter constructs was measured with or without 24-h pretreatment with 2-DG (20 mM) or hypoxia. (hindawi.com)
  • The CTCs and parental MDA-MB-231 cells were incubated at 21 and 0.2% (hypoxia) oxygen, respectively. (nih.gov)
  • Parental MDA-MB-231 cells induced ATF3 in hypoxia, whereas CTCs expressed it constitutively. (nih.gov)
  • Hypoxia induced ATF4 and the HIF1alpha target gene apelin in CTCs, but not in parental cells. (nih.gov)
  • In chronic hypoxia, CTCs demonstrated greater colony formation than parental cells. (nih.gov)
  • Circulating tumour cells (CTCs), isolated and cultured from murine blood, demonstrate a distinct response to hypoxia compared with parental MDA-MB-231cells. (nih.gov)
  • To determine whether CTCs have an altered hypoxia response, they had to be isolated and grown in culture. (nih.gov)
  • For VEGF, the target of current therapies, this correlation between hypoxia and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell lines. (biomedcentral.com)
  • Several clonal lines of cultured Leydig tumor cells have been established and characterized in terms of gonadotropin receptors and steroid production. (nih.gov)
  • Vascular mimicry in cultured head and neck tumour cell lines. (biomedsearch.com)
  • We suggest this is common phenomenon in head and neck squamous cell carcinoma (HNSCC) cell lines and other aggressive tumour cell lines. (biomedsearch.com)
  • We present experimental evidence of vasculogenic mimicry in HNSCC cell lines and compare them with other tumours and a positive control vascular cell line. (biomedsearch.com)
  • Other tumour cell lines also displayed this property. (biomedsearch.com)
  • In future research, Hughes-Fulford will be looking at the overall effect of different types of fatty acids on different tumor types in cell lines as well as human biopsies. (ucsf.edu)
  • Protein extracts were fractionated on Sephadex G-50 columns, and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an African American patient) were treated with MBP extracts from the pooled third peaks. (scirp.org)
  • The focus of this study was to examine the CTC-iChip and determine if the device could properly capture CTCs to aid the formation of cell lines to represent the genetic status of all existing tumor sites. (oncologynurseadvisor.com)
  • Cell lines with longevity were successfully established from 6 patients. (oncologynurseadvisor.com)
  • Additional samples from 3 of these patients were used to set up new cell lines to track the effectiveness of therapy via tumor change. (oncologynurseadvisor.com)
  • Our experimental research benefits from the unrivalled collection of leukemia and lymphoma cell lines stocked at the DSMZ. (dsmz.de)
  • Genomic Landscape of Primary Mediastinal B-Cell Lymphoma Cell Lines. (dsmz.de)
  • The sensitivity of insulin receptors to down regulation by insulin has been measured in cultured human tumor cells (breast tumor cell lines MCF-7, T-47D, and colon tumor cell line HCT-8). (aacrjournals.org)
  • Maximum down regulation of receptors in all three types of tumor cell by prior exposure to insulin did not significantly alter the responsiveness of any of the cell lines to insulin. (aacrjournals.org)
  • whereas, SF50 values were 1,000 nM or more in four (25%: resistant) cell lines. (biomedcentral.com)
  • In addition, knocking down PTEN by siRNA did not alter the sensitivity to olaparib in 2 cell lines with wild-type PTEN. (biomedcentral.com)
  • Therefore, JA induced ER stress in both cell lines, but the consequences of ER stress were different for the epidermal immortalized non-tumorigenic and tumor cells. (springer.com)
  • Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. (semanticscholar.org)
  • Pediatric brain tumor cell lines. (semanticscholar.org)
  • I: Three HER2 overexpressing cell lines were exposed to α-particles from the HER2 binding affibody molecule 211At- (ZHER2:4)2. (eurekaselect.com)
  • The results demonstrated major differences in the VDR levels among the nine cell lines, and VDR positive CTCs were detected in 46% of CTC-positive patients, with a total of 42 CTCs individually analyzed. (mdpi.com)
  • A, chemical structure of nanaomycin A. B, cell viability of HCT116, A549, and HL60 cell lines was determined by counting viable cells after trypan blue staining. (aacrjournals.org)
  • The cellular uptake and metabolism of trans -AVTG in two human renal carcinoma cell lines that were used as models were rapid and associated with depletion of intracellular GSH. (aspetjournals.org)
  • Fifty cell sources, including both immortalized cell lines and primary carcinoma cells, were incubated under normoxic conditions for 48 hours. (biomedcentral.com)
  • We also evaluated the effect of combined therapy with a Hedgehog inhibitor (cyclopamine) in GBM and medulloblastoma lines, and in primary human GBM cultures. (aacrjournals.org)
  • Here, we show that pretreatment of two cervical carcinoma cell lines, HeLa and SiHa, with curcumin before ionizing radiation (IR) resulted in significant dose-dependent radiosensitization of these cells. (aspetjournals.org)
  • While clinically effective therapies targeting HER2 such as mAbs and tyrosine kinase inhibitors exist, tumors overexpressing HER2 eventually progress despite treatment. (biomedcentral.com)
  • This study shows that induction of tumor-specific CD4 + T cells by vaccination with a specific viral T helper epitope, contained within a synthetic peptide, results in protective immunity against major histocompatibility complex (MHC) class II negative, virus-induced tumor cells. (rupress.org)
  • These results indicate the crucial role of T helper cells for optimal induction of protective immunity against MHC class II negative tumor cells. (rupress.org)
  • Evidence for a role of T helper cell-mediated immunity comes from studies with genetically modified tumor cells. (rupress.org)
  • Incubation of the human U937 histiocytic lymphoma cell line with granulocyte-macrophage colony stimulating factor (GM-CSF) rendered the cells responsive to induction of TNF by LPS. (jimmunol.org)
  • B-F) Induction of HIF1α in hypoxic MDA-MB-231 cells and CTCs. (nih.gov)
  • C) Induction of the anoxia-induced factor ATF4 in CTCs compared with MDA-MB-231 cells. (nih.gov)
  • Hypoxic induction of (E) GLUT1 and (F) CAIX, BNIP3 in CTCs, compared with parental MDA-MB-231 cells. (nih.gov)
  • Here, we show that let-7 functionally inhibits non-small cell tumor development. (pnas.org)
  • Here, we report that indeed TA potently and specifically inhibits the chymotrypsin-like activity of purified 20S proteasome (IC 50 = 0.06 μg/ml), 26S proteasome of Jurkat T-cell extracts, and 26S proteasome of living Jurkat cells. (aacrjournals.org)
  • Our present study suggests that TA targets and inhibits the proteasome in tumor cells, which may contribute to the previously observed anticarcinogenic activity of TA. (aacrjournals.org)
  • Here, we report that ester-bond containing TA potently and selectively inhibits the chymotrypsin-like activity in purified 20S proteasome, 26S proteasome of Jurkat T-cell extracts, and 26S proteasome in intact Jurkat cells. (aacrjournals.org)
  • Cotransfection of SP1 cells with green fluorescent protein (GFP)-tagged Tiam1 fragment cDNA and Tiam1 cDNA effectively blocks Tiam1-ankyrin colocalization in the cell membrane, and inhibits GDP/GTP exchange on Rac1 by ankyrin-associated Tiam1 and tumor-specific phenotypes. (rupress.org)