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  • ATCC
  • ATCC Tumor Cell Panels combine authenticated, well-characterized cell lines with mutation data from the Sanger Institute Catalogue of Somatic Mutations in Cancer (COSMIC) to create powerful tools for cancer research and drug discovery. (atcc.org)
  • ATCC has created gene mutation lists based on the ATCC tumor cell line collection and known mutation information maintained in the Sanger Institute COSMIC database. (atcc.org)
  • To enable cancer researchers, ATCC (American Type Culture Collection) has introduced a collection of Tissue-Specific Tumor Cell Panels. (prweb.com)
  • Working closely with customers, ATCC scientists have identified cell lines that that are easy to grow using standard media formulations and possess critical genetic abnormalities found in tumors. (prweb.com)
  • As part of its mission, ATCC will continue to augment the available information on the Tumor Cell Panel cell lines from other databases, and will perform further characterization on the panels. (prweb.com)
  • When combined with the size and scope of the ATCC tumor cell line collection, this growing knowledge base enables scientists to make smarter choices when selecting cell-based research models for cancer research, drug discovery, compound screening, biomarker selection, pathway analysis and functional genomics. (prweb.com)
  • ATCC Tissue-Specific Tumor Cell panels offer economy over individual lines, are supplied with comprehensive genetic profile information, and are accompanied by expert support if needed. (prweb.com)
  • Hep-2 cells ( ATCC CCL-23 ) and HeLa cells ( ATCC CCL-2 ) may be used to propagate virsues that cause bronchopneumonia. (atcc.org)
  • For scientists conducting SARS research, ATCC offers coronaviruses from a range of species and the cell lines needed to cultivate them. (atcc.org)
  • ATCC high-performance media, sera and reagents are recommended for best results and are uniquely formulated according to cell growth recommendations of original cell line depositors and ATCC cell culture specialists. (atcc.org)
  • These high-performance products are the same reagents used to culture and distribute ATCC cell lines, virtually guaranteeing successful cell culture. (atcc.org)
  • ATCC offers a number of commonly used cell culture buffers, stains and dissociation reagents. (atcc.org)
  • ATCC offers an array of products to successfully cryopreserve your cells and microbial cultures. (atcc.org)
  • ATCC offers a variety of dissociation reagents for continuous and primary cell cultures. (atcc.org)
  • ATCC media is the best way to guarantee robust cell growth especially when reviving cells from cryopreservation. (atcc.org)
  • Media supplements and antibiotics from ATCC allow you to customize the growth conditions for your cells. (atcc.org)
  • ATCC offers an assortment of transfection reagents suited to a wide array of cell types. (atcc.org)
  • The cell line was received at the ATCC in January, 1982 at passage 23. (atcc.org)
  • The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. (atcc.org)
  • The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. (atcc.org)
  • malignant
  • The World Health Organization (WHO) classification scheme places neuroendocrine tumors into three main categories, which emphasize the tumor grade rather than the anatomical origin: well-differentiated neuroendocrine tumours, further subdivided into tumors with benign and those with uncertain behavior well-differentiated (low grade) neuroendocrine carcinomas with low-grade malignant behavior poorly differentiated (high grade) neuroendocrine carcinomas, which are the large cell neuroendocrine and small cell carcinomas. (wikipedia.org)
  • Lymphoma is one of the most common malignant tumors to occur in dogs. (wikipedia.org)
  • The enzyme suggested as a tumor marker in malignant disease is the cytosolic cell cycle dependent TK1 that is present during cell division in quantities much larger than TK2 and is therefore set free to the circulation in quantities that completely dominate the activity of thymidine kinase in blood and other body fluids. (wikipedia.org)
  • The thymidine kinase level in serum therefore serves as a measure of malignant proliferation, indirectly as a measure of the aggressivity of the tumor. (wikipedia.org)
  • PCI communicated by malignant cells smothers tumor invasion by hindering urokinase-sort plasminogen activator, and restrains tumor development and metastasis which is independent of its protease inhibitory activity. (wikipedia.org)
  • biomarker
  • A biomarker may be a molecule secreted by a tumor or a specific response of the body to the presence of cancer. (wikipedia.org)
  • DirectHit utilizes monoclonal antibodies (mAb) corresponding to each biomarker to stain the tumor tissue samples. (wikipedia.org)
  • The DirectHit Panel for Breast Cancer was validated in retrospective clinical trials conducted at The University of Maryland Greenebaum Cancer Center and Harborview Cancer Center in Baltimore, MD. One key biomarker was selected as a correspondent to each drug, These biomarkers were identified as playing a significant role in cancer processes and as bearing a mechanistic relation to the action of the specified drug. (wikipedia.org)
  • epidermal growth f
  • Further drug resistance mechanisms analyzed study was the tumor suppressor TP53 and the epidermal growth factor receptor (EGFR). (thefreelibrary.com)
  • 520:163-175 Psyrri A, Yu Z, Weinberger PM: Quantitative Determination of nuclear and cytoplasmic epidermal growth factor receptor expression in oropharyngeal squamous cell cancer by using automated quantitative analysis. (wikipedia.org)
  • novel
  • Altogether, these results suggest that ectopic HER3 expression sensitizes these cells to elisidepsin treatment.DiscussionElisidepsin is a novel marine compound with a potent cytotoxic activity in various tumor cell lines. (urat1inhibitor.com)
  • TCF21 was discovered in 1998 when search for novel cell-type-specific bHLH proteins expressed in human and mouse kidneys by performing a search of the expressed sequence tag (EST) databases. (wikipedia.org)
  • In an effort to identify novel bHLH factors related to dHAND and eHAND (a novel subclass of cell type-restricted bHLH factors shown to play important roles in cardiac morphogenesis), they screened expressed sequence tag (EST) databases for sequences with homology to the bHLH regions of these factors. (wikipedia.org)
  • marker
  • Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line. (atcc.org)
  • and mutations in the tyrosine kinase domain of EGFR1, a marker indicating a patient's non-small-cell lung carcinoma (NSCLC) will likely respond to gefitinib or erlotinib treatment. (wikipedia.org)
  • The potential use as a tumor marker was suggested by Gronowitz et al. (wikipedia.org)
  • Lines
  • These powerful new tools combine well-characterized adherent cell lines that were selected for their genomic mutations as found in the Sanger COSMIC database, greatly reducing the time and effort scientists devote to finding these cells. (prweb.com)
  • Scientists are requiring more genomic and proteomic data on the cell lines they use in their studies to understand the roles that genetic defects have in the pathobiology of cancer," said Dr. Richard Kolodner, Member, Ludwig Institute for Cancer Research, UC San Diego School of Medicine Branch. (prweb.com)
  • The innovative, not-for-profit organization develops and provides products for life science research, services to support biotechnology development, and standards that are consistent with its mission - to acquire, authenticate, preserve, develop, and distribute standard reference microorganisms, cell lines, and related materials for research in the life sciences. (prweb.com)
  • All 60 tumor cell lines displayed significant P450 activity, as well as P450 reductase activity, as determined using the general P450 substrate 7-benzyloxyresorufin. (aspetjournals.org)
  • Although, as noted above, cytochrome P450 enzymes contribute to the metabolism of a large number of drug substrates and can have a large impact on a drug's anticancer activity, little is presently known about the P450 activity levels present in the individual tumor cell lines that constitute the NCI panel. (aspetjournals.org)
  • Panel of tumor cell lines with variable expression of this receptor. (urat1inhibitor.com)
  • To examine this experimentally, we utilized a shRNA construct to stably reduce HER3 expression in a panel of cell lines (Fig. 6).Stable clones of HER3 shRNA and LUC shRNA (control) vectortransfected cells were selected and examined for expression of HER3. (urat1inhibitor.com)
  • Figure 6 shows that cells that have reduced levels of HER3 due to shRNA-mediated knockdown of its expression showed loss of sensitivity to elisidepsin treatment in comparison to control cell lines.To investigate whether ectopic HER3 expression affects the elisidepsin sensitivity of low HER3-expressing cells, the levels of HER3 were increased by transfecting cells with a cDNA encoding HER3, which resulted in increased sensitivity of the cells to elisidepsin. (urat1inhibitor.com)
  • Cell lines more sensitive to elisidepsin had significant HER3 levels. (urat1inhibitor.com)
  • Feeder cell lines, including antibiotic-resistant lines, are tested and validated on multiple stem cell lines for safe and robust stem cell culture. (atcc.org)
  • One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. (atcc.org)
  • Target gene down modulation was studied using qRT-PCR in a panel of tumor cell lines without transfection. (aacrjournals.org)
  • Nanoparticles have shown concentration-dependent and specific target mRNA gene down modulation by qRT-PCR study in a panel of tumor cell lines without transfection. (aacrjournals.org)
  • Absence of HeLa cell contamination in 169 cell lines derived from human tumors. (atcc.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Numerous cell lines are well established as representative of particular cell types. (wikipedia.org)
  • The NCI-60 cancer cell line panel is a group of 60 human cancer cell lines used by the National Cancer Institute (NCI) for the screening of compounds to detect potential anticancer activity. (wikipedia.org)
  • The screening procedure is called the NCI-60 Human Tumor Cell Lines Screen, and it is one of the Discovery & Development Services of NCI's Developmental Therapeutics Program (DTP). (wikipedia.org)
  • Due to the diversity of the cell lines, it is possible to compare tested compounds by their effect patterns, high correlation potentially corresponding to similar effect mechanisms. (wikipedia.org)
  • 13 additional cell lines are evaluated for use in the screening program, among them two lines deriving from so far not represented small-cell lung carcinoma. (wikipedia.org)
  • All cell lines but one (as of 6 January 2017[update]) are available to other laboratories, including the additional cell lines. (wikipedia.org)
  • Starting around the turn of the millennium, several cell lines were found to be misidentified or misclassified at the time of investigation or earlier: MDA-MB-435, originally classified as breast cancer cell line, was identified as being a melanoma cell line. (wikipedia.org)
  • Two brain cancer cell lines, SNB-19 and U251, were discovered to come from the same person. (wikipedia.org)
  • The International Cell Line Authentication Committee maintaines a list of contaminated cell lines. (wikipedia.org)
  • It includes the cell lines reported above, although usually with reporting dates and articles considerably later than the first dates and articles rising specific concern regarding the respective cell line. (wikipedia.org)
  • Similar is true for other misidentified cell lines. (wikipedia.org)
  • Liver
  • There are currently 10 Tissue-Specific Tumor Cell Panels in the collection, including panels for breast cancer, triple-negative breast cancer, lung cancer, ovarian cancer, colon cancer, liver cancer and pancreatic cancer. (prweb.com)
  • The nanoparticles were administered intravenously to tumor-bearing mice to study the bio-distribution and target knockdown in liver and tumor. (aacrjournals.org)
  • Concentrations of equivalent oligonucleotides in tumor, liver, or plasma were evaluated by either ELISA hybridization method or LC-MS method. (aacrjournals.org)
  • After the last dose, tumor and liver samples were collected and analyzed for target mRNA knockdown. (aacrjournals.org)
  • The largest risks for adults who have been studied include several types of leukemia, including myeloid leukemia and acute lymphatic lymphoma as well as tumors of the lung, breast, stomach, colon, bladder and liver. (wikipedia.org)
  • human
  • Improvement in the specific tumor targeting in vivo will practically increase the possibility of using oligonucleotide based molecules to treat a broad spectrum of human diseases. (aacrjournals.org)
  • Enhanced cellular uptake was shown by fluorescent and confocal studies, potent target mRNA down modulation was achieved in a panel of human cancer cells without transfection. (aacrjournals.org)
  • Expression of a constitutively active estrogen receptor variant in the estrogen receptor-negative BT-20 human breast cancer cell line. (atcc.org)
  • The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range. (atcc.org)
  • It has been shown that the deregulation of this critical developmental pathway occurs in several human tumor entities. (wikipedia.org)
  • genetic
  • Beyond enumeration, circulating tumor cells (CTCs) can provide genetic information from metastatic cancer that may facilitate a greater understanding of tumor biology and enable a precision medicine approach. (aacrjournals.org)
  • stated that genetic analysis of chromosome 7, 17, and Y may facilitate discrimination of MA from papillary renal cell carcinoma in difficult cases. (wikipedia.org)
  • potent
  • Potent mRNA knockdown was observed in tumor bearing mice. (aacrjournals.org)
  • Halomon is in a class of chemical compounds known as halocarbons, which are often potent alkylating agents which may be toxic to individual cells or to living organisms. (wikipedia.org)
  • Protein C inhibitor a potent antimicrobial agent that have ability to destroy the bacterial cell wall, causing death of the bacteria. (wikipedia.org)
  • Glioblastoma
  • A TTF-generating device manufactured by the Israeli company Novocure is approved in the United States and Europe for the treatment of newly diagnosed and recurrent glioblastoma multiforme (GBM), and is undergoing clinical trials for several other tumor types. (wikipedia.org)
  • squamous
  • Oropharyngeal squamous cell carcinomas (OSCC) begins in the oropharynx (throat), the middle part of the throat that includes the soft palate, the base of the tongue, and the tonsils. (wikipedia.org)
  • Recombinant
  • Recombinant cyclin/CDK holoenzymes were purified from Sf9 cells engineered to produce baculoviruses that express a specific cyclin or CDK. (aacrjournals.org)
  • gene
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • The majority of research has focused on the Wnt/β-catenin pathway (also known as the "canonical" Wnt pathway), which manages cell fate determination by regulating gene expression. (wikipedia.org)
  • Its restricted expression pattern and DNA binding activity identified Capsulin as a regulator of gene expression in specific subtypes of visceral mesodermal cells involved in organogenesis and in precursor cells that contribute to the pericardium, coronary arteries and regions of the heart. (wikipedia.org)
  • paraffin
  • A) Cell pellets were fixed in formalin, embedded in paraffin and a HER3 IHC was performed. (urat1inhibitor.com)
  • Virtual karyotyping can also be done on fresh or formalin-fixed paraffin-embedded tumors. (wikipedia.org)
  • CLIA-certified laboratories offering testing on tumors include Creighton Medical Laboratories (fresh and paraffin embedded tumor samples) and CombiMatrix Molecular Diagnostics (fresh tumor samples). (wikipedia.org)
  • types
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • It is unknown whether high-LET radiation could cause the same types of tumors as low-LET radiation, but differences should be expected. (wikipedia.org)
  • The types of tumors in humans who are exposed to space radiation will be different from those who are exposed to low-LET radiation. (wikipedia.org)
  • It is ubiquitously expressed in many tissues and cell types and highly significantly expressed in lung and placenta. (wikipedia.org)
  • TCF21 is crucial for the development of a number of cell types during embryogenesis of the heart, lung, kidney, and spleen. (wikipedia.org)
  • RNA in situ hybridization using 33P-labeled riboprobes was used to identify the cell types that expressed Pod-1 in the developing kidney and other tissues. (wikipedia.org)
  • renal cell carc
  • showed that SFRP1 expression in UMRC3 cells (clear cell renal cell carcinoma cell line) resulted in a growth-inhibited phenotype. (wikipedia.org)
  • Metanephric adenoma (MA)is a rare, benign tumour of the kidney, that can have a microscopic appearance similar to a nephroblastoma (Wilms tumours), or a papillary renal cell carcinoma. (wikipedia.org)
  • The symptoms may be similar to those classically associated with renal cell carcinoma, and may include polycythemia, abdominal pain, hematuria and a palpable mass. (wikipedia.org)
  • found that intense and diffuse immunoreactivity for alpha-methylacyl-CoA racemase (AMACR) is useful in differentiating renal cell carcinoma from MA but a panel including AMACR, CK7 and CD57 is better in this differential diagnosis. (wikipedia.org)
  • Their study showed that MA lacks the frequent gain of chromosomes 7 and 17 and losses of the Y chromosome that are typical of papillary renal cell neoplasms, suggesting that MA is not related to renal cell carcinoma and papillary adenoma. (wikipedia.org)
  • tumours
  • G1 and G2 neuroendocrine neoplasms are called neuroendocrine tumors (NETs) - formerly called carcinoid tumours. (wikipedia.org)
  • neoplasms
  • Neuroendocrine tumors (NETs) are neoplasms that arise from cells of the endocrine (hormonal) and nervous systems. (wikipedia.org)
  • Although there are many kinds of NETs, they are treated as a group of tissue because the cells of these neoplasms share common features, such as looking similar, having special secretory granules, and often producing biogenic amines and polypeptide hormones. (wikipedia.org)
  • line
  • The SW 1088 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1975 from an astrocytoma taken from a 72 year old male Caucasian. (atcc.org)
  • This breast tumor line was established by E.Y. Lasfargues and L. Ozzello in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. (atcc.org)
  • This cell line is a suitable transfection host. (atcc.org)
  • The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al. (atcc.org)
  • Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. (atcc.org)
  • The screening rates for each cell line the cytostatic and cytotoxic impact of tested substances. (wikipedia.org)
  • When investigated further, current MDA-MB-435 appeared to be derived from the same individual as cell line M14. (wikipedia.org)
  • NCI/ADR-RES appears to have been derived at some point in time from cell line OVCAR-8. (wikipedia.org)
  • A 61th cell line, MDA-N, has been confirmed to being derived from the misclassified MDA-MB-435 cell line. (wikipedia.org)
  • This cell line is not available as of 6 January 2018[update]. (wikipedia.org)
  • receptor
  • BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5. (atcc.org)
  • EGF receptor is present at 1 X 10 6 per cell. (atcc.org)
  • mammalian cells
  • Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO2 for mammalian cells) in a cell incubator. (wikipedia.org)
  • They differ biochemically from thymidine kinase from mammalian cells and are inhibited by specific inhibitors that do not influence the activity of mammalian thymidine kinases. (wikipedia.org)
  • viability
  • Next, cell viability assays were performed to analyze elisidepsin sensitivity in the generated cells. (urat1inhibitor.com)
  • In comparison to control cells transfected with a LUC vector, decreased cell viability was noted in HER3-transfected cellsEMT and HER3 Predicts Elisidepsin SensitivityFigure 4. (urat1inhibitor.com)
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • resistance
  • P-glycoprotein- overexpressing CEM/ADR5000 cells were not cross-resistant to honokiol, but MDA-MB-231 BRCP cells transfected with another ABC-transporter, BCRP, revealed 3-fold resistance. (thefreelibrary.com)
  • did not reveal resistance to honokiol, and EGFR-transfected U87.MG EGFR cells were collateral sensitive compared to wild-type cells (degree of resistance: 0.34). (thefreelibrary.com)
  • Drug resistance is frequently multifactorial and several mechanisms account for unresponsiveness of tumors towards drugs. (thefreelibrary.com)
  • origin
  • Traditionally, neuroendocrine tumors have been classified by their anatomic site of origin. (wikipedia.org)
  • The epitheliotropic form is typically of T-cell origin and is also called mycosis fungoides. (wikipedia.org)
  • The non-epitheliotropic form is typically of B-cell origin. (wikipedia.org)