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  • endothelial cells
  • Target selectivity and extent of internalization were evaluated on monolayers of human endothelial cells (HUVEC) induced to express PSMA in static incubation conditions and in a flow field. (aacrjournals.org)
  • Human tumor endothelial cells expressing human antigens of targeting interest are practically still not available in culture ( 6 ), and in animal models the neovasculature and its antigens are of host-origin. (aacrjournals.org)
  • To emulate tumor endothelium analogues in vitro , we utilize a parallel-plate flow chamber with a controlled flow field containing the targeted therapeutics and with walls coated with monolayers of model human endothelial cells (HUVEC) induced to express the prostate-specific membrane antigen (PSMA). (aacrjournals.org)
  • Nuclear
  • Removal of histones and other nuclear proteins greatly enhances the sensitivity of mammalian cells to DNA damage by ionizing radiation. (semanticscholar.org)
  • We examined the possibility that the ease of dissociation of histones, or the association of other nuclear proteins with DNA, may differ between radioresistant and sensitive human tumor cells. (semanticscholar.org)
  • In particular, it defines a pipeline-path that excludes nonliver cell nuclei in two consequent pipeline-modules and utilizes the liver cell nuclear features for HCC grading. (spie.org)
  • Four categories of liver cell nuclear features were utilized for classifying the HCC tumors. (spie.org)
  • Small, dark nuclear remnants seen within red cells in PBS of hereditary spherocytosis. (brainscape.com)
  • The clear cell variant of rhabdomyosarcoma is rarely documented, but this case shows a highly unusual finding in which the rhabdomyoblasts show the prominent multivacuolation with nuclear indentation characteristic of and indistinguishable from pleomorphic lipoblasts. (hindawi.com)
  • tissues
  • It is important to diagnose low-grade HCC tumors because these tissues have good prognosis. (spie.org)
  • Trainees will learn how to prepare cells and tissues for 3D imaging, and how to perform fluorescent in situ hybridization (FISH) followed by 3D imaging and analysis. (umanitoba.ca)
  • Actinium-225 emits a total of four α-particles per decay, providing highly lethal and localized irradiation of targeted cells with minimal exposure to surrounding healthy tissues. (aacrjournals.org)
  • membrane
  • These results provide insights into a new pathway by which mitochondrial DNA and membrane damage can contribute to tumor progression and metastasis. (nih.gov)
  • Changes in mitochondrial membrane potential in mtDNA-depleted and reverted cells. (nih.gov)
  • The mechanism of its entry is poorly understood, but recent studies indicate that the oleic acid in the HAMLET complex interacts with phosphatidylserine and o-glycosylated mucin on the plasma membrane, both of which are expressed in greater amounts on the plasma membrane of tumor cells, possibly providing for HAMLET's specificity. (wikipedia.org)
  • HAMLET cells showed the physiological characteristics of macroautophagy , a process in which cellular components are sequestered in double membrane-bound vesicles that fuse with lysosomes for degradation. (wikipedia.org)
  • Hemolysis that can result from mechanical trauma, or biochemical or physical agents that damage the red cell membrane. (brainscape.com)
  • This disorder is characterized by an intrinsic defect in the red cell membrane, that renders the cells spheroidal, less defomable and vulnerable to splenic sequestration and destruction. (brainscape.com)
  • Nanocarriers (lipid vesicles) targeted to the prostate-specific membrane antigen (PSMA), which is unique to human neovasculature for a variety of solid tumors, were loaded with the α-particle generator actinium-225 and were compared with a PSMA-targeted radiolabeled antibody. (aacrjournals.org)
  • LEthal
  • HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is a complex between alpha-lactalbumin and oleic acid that induces cell death in tumor cells, but not in healthy cells. (wikipedia.org)
  • The super-lethal nanoparticles reduced tumor growth and extended survival rates. (phys.org)
  • For the first time, researchers combined traditional chemotherapy with a relatively new cancer drug that attacks chemo-resistant tumor cells, loaded both into tiny nanoparticles, and created an extremely selective and lethal cancer treatment . (phys.org)
  • The mutation, known as Loss of Function p53, interrupts the normal life cycle of tumor cells and makes them more resistant to the lethal effects of chemotherapy. (phys.org)
  • We call this a synthetically lethal situation because we are creating the right conditions for massive cell death. (phys.org)
  • Critical prerequisites in this scenario, however, include the selective targeting of the tumor vasculature and the targeted delivery of highly lethal therapeutics. (aacrjournals.org)
  • of the order of 80 keV/μm) and short range (50-100 μm) of α-particles resulting in highly lethal and localized irradiation of the tumor vasculature. (aacrjournals.org)
  • progression
  • EphA2 deficiency impaired tumor initiation and metastatic progression in mice overexpressing ErbB2 (also known as Neu) in the mammary epithelium (MMTV-Neu mice), but not in mice overexpressing the polyomavirus middle T antigen in mammary epithelium (MMTV-PyV-mT mice). (nih.gov)
  • These data suggest that EphA2 cooperates with ErbB2 to promote tumor progression in mice and may provide a novel therapeutic target for ErbB2-dependent tumors in humans. (nih.gov)
  • Moreover, EphA2 function in tumor progression appeared to depend on oncogene context, an important consideration for the application of therapies targeting EphA2. (nih.gov)
  • Inhibition
  • However, proteasome inhibition alone does not seem to be responsible for HAMLET-induced cell death, as proteasome inhibitors have been shown to reduce the cytotoxicity of HAMLET. (wikipedia.org)
  • However, recent studies highlight that Merlin pleiotropically affects signalling by migrating into the nucleus and inducing a growth‐suppressive programme of gene expression through its direct inhibition of the CRL4 DCAF1 E3 ubiquitin ligase. (embopress.org)
  • mRNA
  • Reverted cells with approximately 70% of control cell mtDNA exhibited marker mRNA contents, cell morphology and invasive property closer to control cells. (nih.gov)
  • A ) Differential display pattern showing induced level of cathepsin L mRNA in mtDNA-depleted cells. (nih.gov)
  • Analyses
  • Biochemical analyses revealed that EphA2 formed a complex with ErbB2 in human and murine breast carcinoma cells, resulting in enhanced activation of Ras-MAPK signaling and RhoA GTPase. (nih.gov)
  • targets
  • One of the most prominent targets of HAMLET once inside the cell is the mitochondrion . (wikipedia.org)
  • HAMLET also targets the nucleus, where it interacts with histones to interfere with transcriptional processes. (wikipedia.org)
  • Besides limiting blood vessel growth , nintedanib also targets tumor cells with a specific mutation. (phys.org)
  • demonstrate
  • E ) H&E-stained tumor sections (1 yr) demonstrate increased cystic degeneration and lumen formation in EphA2 -/- tumors. (nih.gov)
  • These findings demonstrate that radionuclide carriers, such as PSMA-targeted lipid-nanocarriers, which localize close to the nucleus, increase the probability of α-particle trajectories crossing the nuclei, and, therefore, enhance the killing efficacy of α-particle emitters. (aacrjournals.org)
  • synthetic lethality
  • Ebeid says this is the first time that researchers have used nintedanib to force tumor cells into mitosis and kill them-a phenomenon scientists refer to as "synthetic lethality. (phys.org)
  • BMN 673 has been shown to cause single-agent synthetic lethality in BRCA1/2- and PTEN-deficient cell lines, and has potent antitumor activity in animal models of tumors harboring mutations in DNA repair pathways. (clinicaltrials.gov)
  • pulmonary
  • Feared complication of sickle cell disease which can be trigerred by pulmonary infections or fat emboli from necrotic marrow that secondarily involve the lung. (brainscape.com)
  • inflammatory
  • To examine whether an inflammatory cytokine is expressed in the nucleus pulposus of adolescent patients with lumbar disc herniation. (ovid.com)
  • Acute severe asthma involves inflammatory cell infiltration accompanied by airway inflammation, mucous plugging, proteinaceou. (indexedvisuals.com)
  • markers
  • Tumor-specific markers cathepsin L and transforming growth factor beta (TGFbeta) are overexpressed in cells subjected to mitochondrial genetic as well as metabolic stress. (nih.gov)
  • human
  • Dynamic illustration depicts the life cycle of the AIDS/ HIV (human immunodeficiency) virus as it enters a human T-cell, reve. (indexedvisuals.com)
  • nonneural human and animal tumors. (bio.net)
  • The quantitative measures of ER examined here included the percentage of tumor cell nuclei positively stained for ER, ER Histo (H)-score, and a score based on an adaptation of an equation presented by Cuzick and colleagues, which combines weighted values of ER H-score, percentage of tumor cell nuclei positively stained for the progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) results. (nih.gov)
  • differentiation
  • Thus, although initially thought to be of mesothelial origin due to sites of presentation, it is now hypothesized to arise from a progenitor cell with multiphenotypic differentiation. (wikipedia.org)
  • quantitative
  • The two quantitative measures for ER alone provided additional discrimination in breast cancer-specific mortality risk only among white women with ER + tumors (both Ptrend ≤ 0.01) while the ER/PR/HER2 score provided additional discrimination for both white women (Ptrend = 0.01) and black women (Ptrend = 0.03) with ER + tumors. (nih.gov)
  • relatively
  • Researchers then fueled the nanoparticles with two anti-cancer drugs: paclitaxel, a type of chemotherapy used to treat endometrial cancer, and nintedanib, or BIBF 1120, a relatively new drug used to restrict tumor blood vessel growth. (phys.org)
  • solid
  • Determine the pharmacodynamic effect of BMN 673 in tumor biopsies from patients with advanced ovarian, breast, or other solid tumor and deleterious BRCA mutations. (clinicaltrials.gov)
  • Adult patients with documented deleterious BRCA 1 or 2 mutations with histologically confirmed ovarian, primary peritoneal, breast, prostate, pancreas, gastric or other solid tumor whose disease has progressed following at least one standard therapy or who have no acceptable standard treatment options. (clinicaltrials.gov)
  • The patient was commenced on 3 cycles of neoadjuvant carboplatin and paclitaxel, after which CT scan showed partial response with reduction of tumor size (particularly of the solid component) from 11 cm to 8 cm and reduction in size of abdominopelvic nodes. (hindawi.com)
  • acute
  • Major complication of sickle cell disease which occurs in the setting of acute chest syndrome, causing ischemic injury to the CNS. (brainscape.com)
  • cancer
  • HAMLET is a possible chemotherapeutic agent with the ability to kill cancer cells. (wikipedia.org)
  • Tumor-targeting nanoparticles loaded with a drug that makes cancer cells more vulnerable to chemotherapy's toxicity could be used to treat an aggressive and often deadly form of endometrial cancer, according to new research by the University of Iowa College of Pharmacy. (phys.org)
  • In this particular study, we took on one of the biggest challenges in cancer research, which is tumor targeting," said Kareem Ebeid, a UI pharmacy science graduate student and lead researcher on the study. (phys.org)
  • And for the first time, we were able to combine two different tumor-targeting strategies and use them to defeat deadly type II endometrial cancer. (phys.org)
  • The tumor appears to arise from the primitive cells of childhood, and is considered a childhood cancer. (wikipedia.org)
  • After controlling for age at diagnosis, race, study site, tumor stage, and histologic grade in multivariable Cox proportional hazards regression models, both percentage of tumor cell nuclei positively stained for ER (Ptrend = 0.0003) and the ER H-score (Ptrend = 0.0004) were inversely associated with breast cancer-specific mortality risk. (nih.gov)
  • The ER/PR/HER2 score was positively associated with breast cancer-specific mortality risk in women with ER + tumor (Ptrend = 0.001). (nih.gov)
  • Percentage of tumor cell nuclei positively stained for estrogen receptor (ER) (A) and ER Histo (H)-score (B) in 1,204 white women and black women diagnosed with invasive breast cancer. (nih.gov)
  • Percentage of tumor cell nuclei positively stained for estrogen receptor and breast cancer-specific mortality risk. (nih.gov)
  • Adjusted hazard ratio (HR) estimates (95% confidence intervals (CIs)) of breast cancer-specific mortality associated with the percentage of tumor cell nuclei positively stained for estrogen receptor (ER) in all women (A) , in white women (B) , and in black women (C) with invasive breast cancer. (nih.gov)
  • oncogene
  • These recent advances suggest that Merlin acts at the cell cortex and in the nucleus in a similar, albeit antithetic, manner to the oncogene β‐catenin. (embopress.org)
  • assess
  • New methods are being developed to assess tumor vascularity by MRI, including techniques such as arterial-spin labeling (ASL) and dynamic contrast-enhanced MRI. (medscape.com)
  • attacks
  • Nintedanib attacks tumor cells with the Loss of Function p53 mutation and compels them to enter mitosis and divide, at which point they are more easily killed by chemotherapy. (phys.org)
  • small
  • Nanoparticles are small enough to slip through the holes, thereby allowing them to specifically target tumors. (phys.org)
  • Methods
  • B ) Flow cytometry analysis of cells stained with mitochondrial-specific cationic dye, Mitotracker, as described in Materials and methods. (nih.gov)
  • D ) The level of intracellular and extracellular cathepsin L activity was assayed in total cell extract or concentrated culture fluid as described in Materials and methods. (nih.gov)
  • By using DNA probes and microscopy-based methods, we explore the structure of the nucleus and the organization of the genome. (umanitoba.ca)
  • New methods of tumor imaging are being developed to specifically tag or label tumor cells so they may be visualized in the operating room. (medscape.com)
  • Such methods include pretreatment of the patient with dyes or tumor-specific proteins tagged with fluorescent molecules. (medscape.com)
  • Fluorescence
  • A-E ) Two RB1-immunonegative tumors were either homozygous ( A, B ) or hemizygous ( C, D ) deleted for RB1 by fluorescence in situ hybridization. (nih.gov)