Tumor cell hybrid cells. Medical search. Frequent questions

A Hybrid Multiscale Model of Tumour Invasion: Evolution and the Microenvironment. (maa.org)
Moreover, iNOS+ARG1+ double-positive macrophages are present in the microenvironment of pancreatic tumors. (aacrjournals.org)

Glioblastoma (GBM) is a very aggressive primary malignant brain tumor and finding effective therapies is a pharmaceutical challenge and an unmet medical need. (frontiersin.org)
Firstly, the anatomical location and size of the tumor impact the extent of resection, as a fine balance must be achieved between the maximal removal of malignant tissue and minimal operative risk ( 6 ). (frontiersin.org)
Hybrids in which malignancy was suppressed and malignant segregants derived from them showed virtually identical SCE frequencies. (biologists.com)
These are independent clonal derivatives of a spontaneous malignant melanoma tumour in the mouse. (biologists.com)
A malignant tumor is a cluster of abnormal cells that are growing out-of-control, disrupting the normal operation of nearby healthy tissue. (gatech.edu)
MAGE proteins are usually restricted in their expression to malignant or tumour cells, however NRAGE was also expressed in terminally differentiated adult tissue. (uni-wuerzburg.de)

Just as bacteria, viruses and fungi develop strategies to outsmart antimicrobial medications, cancer cells can become resistant to chemotherapy. (medicalxpress.com)
Traditionally, the treatment of hematological malignancies is administrated by chemotherapy, radiotherapy, and stem cell transplantation. (hindawi.com)
The standard of care therapy for GBM consists in surgical resection of the tumor, followed by radiotherapy and adjuvant plus concomitant chemotherapy with Temozolomide (TMZ) (Stupp protocol) ( 3 ). (frontiersin.org)
In conclusion, liposomal Bamet-UD2 may be a useful tool to circumvent resistance to chemotherapy, particularly in tumors of the enterohepatic circuit. (aspetjournals.org)
Treatment for patients (who tend to be older adults) involves surgery to remove as much of the tumour as possible followed by chemotherapy and radiotherapy. (neurosciencenews.com)

Quantification of cell-cycle state at a single-cell level is essential to understand fundamental three-dimensional (3D) biological processes such as tissue development and cancer. (nature.com)
During the course of minimally invasive tumor resection, it is often difficult for the surgeon to clearly distinguish between healthy and sick tissue on the endoscopic image. (fraunhofer.de)
Project C-VIS concerns an alternative method which in a short period of time can directly help visualize the tumor tissue in the body. (fraunhofer.de)
For example, these biomimetic constructs are able to detect tumor tissue and distinguish it from healthy tissue. (fraunhofer.de)
However, CAR-T cells can also produce some adverse events after treatment of hematological malignancies, such as cytokine release syndrome (CRS), neurotoxicity, and on-target/off-tumor toxicity, which may cause systemic immune stress inflammation, destruction of the blood-brain barrier, and even normal tissue damage. (hindawi.com)
For example, a subset of astrocytoma cells with ultra-long, thin, and highly dynamic membrane protrusions named tumor microtubes are able to extend in the surrounding tissue for tumor cell invasion, proliferation, and connection between tumor cells ( 11 ). (frontiersin.org)
A new study published in Cell has found a previously unknown group of cells that have a greater ability to regenerate liver tissue in mice without forming tumors compared to ordinary liver cells, known as hepatocytes. (doctorshealthpress.com)
Researchers examined liver cells involved in regenerating tissue following injuries that were caused by carbon tetrachlorideexposure, an environmental toxin. (doctorshealthpress.com)
For chronic liver injuries, hybrid hepatocyte cells reproduce efficiently and replenish tissue in the liver. (doctorshealthpress.com)
A great many noxious agents capable of inducing SCE in tissue culture cells are carcinogenic (see, for example, Wolff, 1977 ). (biologists.com)
A new, freely available computer platform creates a 3D model that brings together the macroscopic scale of tissue with the microscopic scale of individual cells. (neurosciencenews.com)
Publishing today in the journal Methods, the team from Newcastle University, the University of Cyprus, UCL and CERN describe how they have developed the three-dimensional model which brings together the macroscopic scale of tissue with the microscopic scale of individual cells. (neurosciencenews.com)
Unlike laser light, used in photodynamic cancer therapy, ultrasound waves can reach deep into tissue-up to 12 cm-to treat deep tumors without damaging healthy cells. (phys.org)
A new agent that accumulates in the tumor tissue and is activated there by ultrasound waves does not have this problem. (phys.org)
We evaluated a hybrid catalytic power source for less invasive internal electroporation with better tissue reach than the widely used and more invasive external electroporation. (scielo.org.za)
tissue dose and tumor response among species have been flux and is thereby a basis for describing the effects of anatomy and airflow on local tissue disposition and distributions of tissue significant sources of uncertainty in all these assessments. (cdc.gov)

THC-specific cell surface signature CD36 CD14 PANK allows identifying them in matched primary tumor tissues and metastases as well as in bloodstream from patients with lung cancer, thus functioning as a biomarker. (uam.es)
Photothermal therapy may be a promising strategy for the treatment of GBM, as it allows the destruction of the tumor using heat as a non-chemical treatment for disease bypassing the GBM heterogeneity limitations, conventional drug resistance mechanisms and side effects on peripheral healthy tissues. (frontiersin.org)
The Cellular Potts Model and Biophysical Properties of Cells, Tissues and Morphogenesis. (maa.org)
Modelling Development of Complex Tissues using Individual Viscoelastic Cells. (maa.org)
For the current study, the research team looked at molecular differences between initial, or primary, triple-negative breast cancer sites and areas where it spread, or metastatic sites, among three different types of cells: mouse models, human cancers implanted into mice and samples of both primary and metastatic tissues taken from eight patients treated at The Johns Hopkins Hospital. (sciencedaily.com)
The scientists found that when triple negative breast cancer cells invade other tissues on their way to another part of the body, they gain two cellular properties: better movement and survival. (sciencedaily.com)
When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Tissues and Cells. (lu.se)
The immune system is comprised of highly specialized q The findings and conclusions in this report are those of the authors and cells, tissues, and organs that give the human body the do not necessarily represent the views of the Agency for Toxic Substances and Disease Registry. (cdc.gov)

The review highlights the discovery of oncogenes and suppressor tumor genes, underlining the crucial role of these achievements in cancer diagnosis and therapies. (jcancer.org)

The C-VIS method is based on the property of modified nanoparticles attaching to tumor cells, but not to healthy cells. (fraunhofer.de)
To this purpose, we at Fraunhofer IGB are producing nanoparticle hybrid systems consisting of synthetic nanoparticles and biologically active proteins. (fraunhofer.de)
Spraying of the nanoparticles: The marked TNF-functionalized silica nanoparticles bind to cells. (fraunhofer.de)
If these prove successful, the next step will focus on developing different nanoparticles to detect specific tumors, investigating solutions for automatic image evaluation and pursuing the options for precise spatial localization of the tumor. (fraunhofer.de)
Photoabsorbing agents such as nanoparticles need to reach the tumor site at therapeutic concentrations, crossing the blood-brain barrier upon systemic administration. (frontiersin.org)

Hence, it is important to develop tumor cell-targeted radiotherapy that could improve radiotherapy efficiency on tumor cells and reduce the toxic effect on normal cells during radiation treatment. (tmu.edu.tw)
CSAF NPs were employed together with 5-aminolevulinic acid (5ALA) to achieve tumor cell-targeted radiotherapy. (tmu.edu.tw)
The results provide an essential basis for developing tumor-cell targeted radiotherapy by means of low-dose radiation. (tmu.edu.tw)

The first instance of intentionally generated hybrid cells was described in 1960 by Barski, Sorieul, and Cornefert in their paper "Production of cells of a 'hybrid' nature in cultures in vitro of 2 cellular strains in combination," originally published in French. (wikipedia.org)
In this study, osteosarcoma cells (MG63) and normal cells (MC3T3-E1) were used for an in vitro investigation, in which reactive oxygen species (ROS) assay, cell viability assay, clonogenic assay, and western blot were used to confirm the treatment efficiency. (tmu.edu.tw)
2012 ). In vitro , flavopiridol suppresses the interaction between leukocytes and endothelial cells in the model of murine hepatitis, which is a crucial step in the initiation of inflammation (Schmerwitz et al. (researchsquare.com)
The problem we have been concerned with is to find an in vitro correlate with tumorigenicity in a system of cell hybrids in the mouse. (biologists.com)
Cytotoxic T lymphocytes were generated in vitro against H-2 compatible or syngeneic tumor cells. (rupress.org)
In vitro cytotoxic activity was inhibited by specific anti-H2 sera, suggesting that H-2 antigens are involved in cell lysis. (rupress.org)
Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
In vitro and in vivo studies were conducted to investigate the antitumor mechanism of A3@GMH in combination with photothermal therapy(PTT) through inducing immunogenic cell death(ICD) in 4T1 cells. (bvsalud.org)
A3@GMH actively mediated oncolytic features such as 4T1 cell lysis and LDH release, as well as ICD effects, and showed enhanced in vitro antitumor activity when combined with PTT. (bvsalud.org)

Our results suggest that THCs are involved in both origin and spread of metastatic cells. (uam.es)
Background: Circulating tumor cells (CTCs) are responsible for the metastatic dissemination of colorectal cancer (CRC) to the liver, lungs and lymph nodes. (unicatt.it)
Conclusions: These results indicate that CTCDOs recapitulate several features of colorectal CTCs and may be used to investigate the features of metastatic CRC cells, to identify new prognostic biomarkers and to devise new potential strategies for metastasis prevention. (unicatt.it)
CTCs analysis through liquid biopsies provides not only a full repertoire of tumor biological material (including proteins, lipids, sugars and nucleic acids) but also a sample of living tumor cells endowed with metastatic ability [ 3 ]. (biomedcentral.com)
The researchers used a combination of machine learning, cellular imaging, and biochemical analysis to identify differences in the genetic expression patters of initial and metastatic tumors. (sciencedaily.com)
The bad news from our study is that cells from metastatic sites are super optimized for migration and resisting treatment," says Ewald. (sciencedaily.com)
The good news is that we identified several proteins called transcription factors that these cells require to handle the challenges of migrating and thriving at metastatic sites, and we may be able to design new therapies that target these transcription factors. (sciencedaily.com)
To look more closely at molecules involved in hybrid EMT states, the scientists sought the help of Elana Fertig, Ph.D., division director and associate director of quantitative sciences and co-director of the Convergence Institute at the Johns Hopkins Kimmel Cancer Center, to track the molecular patterns of individual cells in cell assays that model invasion out of the primary tumor and formation of a colony in a metastatic site. (sciencedaily.com)
The scientists found that most of the metastatic cells morph into the more mobile, better surviving, hybrid EMT state. (sciencedaily.com)
Ackerman used the term verrucous carcinoma to denote the concept of a locally aggressive exophytic low-grade squamous cell carcinoma with little metastatic potential. (medscape.com)

We present a spatial hybrid discrete-continuum modelling framework for the interaction dynamics between tumour cells and cytotoxic T cells, which play a pivotal role in the immune response against tumours. (hal.science)
Using tumor cells constitutively expressing a cytomegalovirus (CMV) antigen, we show that expansion of CMV-specific cytotoxic T lymphocytes (CTLs) restricted by DCs' MHC class I molecules was higher when DC-tumor hybrids were the stimulators. (ox.ac.uk)
The most active T cell endogenous inhibitory pathway is the immunoglobulin superfamily such as CD28/cytotoxic T lymphocyte antigen-4 (CTLA-4): B7-1/B7-2 receptor/ligand grouping, which plays a central role in coordinating immune responses [ 7 , 8 ]. (hindawi.com)
Reprogramming is a promising treatment, which redifferentiates T-induced pluripotent stem cells (T-IPSCs) into naïve and cytotoxic T cells or dedifferentiates within their own lineage [ 13 ]. (hindawi.com)
First, coating of the H-2 antigens on the target tumor cell reduced the number of cells lysed on subsequent exposure to cytotoxic T cells. (rupress.org)
Second, when cytotoxic T cells were activated against an H-2 compatible tumor and assayed against an H-2-incompatible tumor, anti-H-2 serum that could bind to the target cell, but not to the cytotoxic lymphocyte, inhibited lysis. (rupress.org)
These findings suggest that it is the H-2 antigens on the tumor cell and not those on the cytotoxic lymphocytes that are important in cell-mediated lysis of H-2-compatible tumor cells. (rupress.org)

Hybrid cell lines are also used to study cancer and map genes The three most common methods of fusing cells to make a hybrid line are via oncogenic viruses, polyethylene glycol, or electrofusion. (wikipedia.org)
When the viral genes are expressed by the host cell, the membranes of two cells may fuse. (wikipedia.org)
Methods TCR sequences were detected using the Tempus xR assay, a hybrid-capture whole exome RNA assay optimized with probes for capture of TCR genes. (bmj.com)
The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)
While they are very similar to hepatocytes, hybrid hepatocytes were found to have much lower levels of bile duct cell-specific genes. (doctorshealthpress.com)
Nowell, a tumor biologist in the pathology department at the University of Pennsylvania School of Medicine, was interested in the relationship between cancer and alterations in genes (although he had no proof there was one). (laskerfoundation.org)
Remarkably, hybrid molecules fail to form in tumor cells treated with EFV under the same conditions that repress proliferation and induce the reprogramming of expression profiles of coding genes, microRNAs (miRNAs) and ultraconserved regions (UCRs). (unife.it)
Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)

Its power intensity needs to be within the safety limit and its penetration depth should be sufficient to induce deep and localized hyperthermia and achieve tumor destruction. (frontiersin.org)

Anatomopathology confirmed complete resection of the tumor reaching the muscle layer and the perivesical fat. (cdc.gov)
With this information, he can treat the tumor and the resection margins in a targeted manner. (fraunhofer.de)

Certain oncogenic viruses encode for fusogens, which are proteins that encourage two cell membranes to fuse. (wikipedia.org)
CTCDOs recapitulated several features of CRC CTCs including a hybrid epithelial-mesenchymal transition (EMT) state and elevated expression of stemness-associated proteins. (biomedcentral.com)
Um diese Befunde genauer zu untersuchen, wurde in dieser Arbeit ein "yeast two-hybrid screen" mit ITA als "bait" (Köder) und einer cDNA-Bank aus Die Familie der „inhibitor of apoptosis proteins" (IAPs) interagieren mit einer wachsenden Zahl an intrazellulären Proteinen und Signaltransduktionswegen um ihre anti-apoptotische Aufgabe zu erfüllen. (uni-wuerzburg.de)
We found that the three Id proteins expressed in neuroblastoma cells (Id1, Id2, and Id3) were down-regulated during induced differentiation, indicating that Id proteins help keep the tumor cells in an undifferentiated state. (lu.se)
The ability of Id proteins to affect HES-1 activity is of particular interest in neuronal cells, where regulation of HES-1 is essential for the timing of neuronal differentiation. (lu.se)

Neuroblastoma is a devastating solid tumor of childhood and emerges in the sympathetic nervous system while many kids who develop it are still toddlers. (medicalxpress.com)
Scientists at the Indian Institute of Science (IISc) have developed a new approach to potentially detect and kill cancer cells, especially those that form a solid tumor mass. (phys.org)

At the same time, a subpopulation of highly tumorigenic glioma stem cells (GSCs) with high plasticity and self-renewal properties contribute to tumor malignancy through their sustained proliferation, invasion, stimulation of angiogenesis, suppression of anti-tumor immune responses and chemo-resistance ( 12 ). (frontiersin.org)
This study suggests that the combination of stapled anoplin peptide and PTT significantly enhances the oncolytic immunotherapy for TNBC and provides a basis for the innovative application of anti-tumor peptides derived from TCM in TNBC treatment. (bvsalud.org)

To understand why this is so important, it helps to understand the chemistry of brain tumor creation. (gatech.edu)

Additionally, GBM is characterized by high inter-tumor and intra-tumor heterogeneity at a cellular, molecular, histological, and clinical level leading to very different responses to therapeutic agents and failure of targeted therapies ( 9 , 10 ). (frontiersin.org)
The researchers hope this study will lead the way to cell-based therapies that could replace the need for liver transplants in the future. (doctorshealthpress.com)
Many current breast cancer therapies work by targeting those flags, rendering them of little use to those with triple negative tumors. (sciencedaily.com)
Macrophages are cells vital to the immune system and could possibly inform cell-based therapies for a variety of medical conditions. (phys.org)

Moreover, mice models were assessed for in vivo characterization of hybrids colonization and invasiveness. (uam.es)
Despite recent advances in repertoire sequencing methods, the characterization of tumor-infiltrating T-cell repertoires has been limited by access to large, real-world patient cohorts with genomic, TCR, and clinical profiling. (bmj.com)

We have established an experimental model of CTCs-derived organoids (CTCDOs) obtained from circulating cancer cells spontaneously generated in an orthotopic CRC xenograft model derived from one CRC patient. (biomedcentral.com)
Meriolin 3 potently inhibits tumor growth in two mouse xenograft cancer models, namely, Ewing's sarcoma and LS174T colorectal carcinoma. (rcsb.org)

However, it seems to be one of the best explanations for both the origin and metastasis of primary tumors. (uam.es)
Circulating tumor cells (CTCs) contain the physical elements responsible for tumor dissemination and metastasis. (biomedcentral.com)
Medical Xpress) -- One of cancer's greatest and most insidious threats is metastasis the three-dimensional migratory invasion of cancer cells from primary tumors to a distant part of the body. (medicalxpress.com)
We have long needed new treatment targets and options for triple negative breast cancers," says Andrew Ewald, Ph.D., the Virginia DeAcetis Professor in Basic Science Research and Director, Department of Cell Biology at the Johns Hopkins University School of Medicine and co-leader of the Cancer Invasion and Metastasis Program at the Johns Hopkins Kimmel Cancer Center. (sciencedaily.com)

At a time when the idea that cancer had a genetic basis was widely disbelieved, Nowell's results provided the first clear evidence that a particular genetic defect in a single chromosome can lead to a population or clone of identical cells that accumulate in numbers to form a deadly malignancy. (laskerfoundation.org)

Polyethylene glycol functions by changing the direction and configuration of lipid molecules in the cell membrane, which increases their permeability and allows two membranes to fuse. (wikipedia.org)
RESULTS: Less than half the cells in the mixture expressed DC co-stimulatory molecules. (ox.ac.uk)
Hybrids expressed more co-stimulatory and MHC molecules, induced higher antigen-specific T-cell expansion and were the only cells able to induce IFN-γ-producing antigen-specific T cells. (ox.ac.uk)
Meriolins display better antiproliferative and proapoptotic properties in human tumor cell cultures than their parent molecules, meridianins and variolins. (rcsb.org)
Using CsCl density gradients, we selectively identify Alu and LINE-1 containing DNA:RNA hybrid molecules in cancer but not in normal cells. (unife.it)
Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)

Circulating cell-free DNA (cfDNA) has the potential to be a specific biomarker for the therapeutic management of lung cancer patients. (nature.com)

The 'cancer cell fusion' theory is controversial due to the lack of methods available to identify hybrid cells and to follow the phenomenon in patients. (uam.es)
Herein, we co-cultured lung cancer stem cells with human monocytes and analyzed the dynamics and properties of tumor-hybrid cells (THC), as well as the molecular mechanisms beneath this fusion process by several techniques: electron-microscopy, karyotyping, CRISPR-Cas9, RNA-seq, immunostaining, signaling blockage, among others. (uam.es)
We applied our workflow to quantify cell-cycle effects of three antimitotic cancer drugs over 8 d in HT-1080 fibrosarcoma xenografts in living mice using a data set of 38,000 cells and compared the induced phenotypes. (nature.com)
Hybridoma: a fusion of B-cells and immortal cancer cells with the purpose of generating antibodies. (wikipedia.org)
Cancer Cell International. (wikipedia.org)
BACKGROUND AIMS: Dendritic cell (DC)-tumor cell hybrids have been used clinically in cancer immunotherapy, but their advantage over the simple mixture of tumor cells and DCs is still a matter of controversy. (ox.ac.uk)
Thus, these data offer further support for cancer immunotherapeutic approaches using DC-tumor cell hybrids. (ox.ac.uk)
CAR T cell therapy, the breakthrough method of supercharging patients' T cells in the lab to attack cancer when re-infused, has worked more effectively against cancers of the blood than solid tumors. (medicalxpress.com)
Although immunotherapy has achieved increasing prominence in the panoply of innovative cancer treatments, it remains an imperfect tool-too many tumors simply do not respond. (medicalxpress.com)
Cancer-associated fibroblasts suppress ferroptosis and induce gemcitabine resistance in pancreatic cancer cells by secreting exosome-derived ACSL4-targeting miRNAs. (nih.gov)
Molecular profiling of a cancer patient's tumor to reveal targetable alterations is an important first step in the personalization of cancer treatment plans. (nature.com)
Biopsies of advanced stage non-small cell lung cancer (NSCLC) need invasive exams in fragile patients, and therefore minimally invasive "liquid biopsies" have generated considerable enthusiasm. (nature.com)
Novel understanding in the interaction between immune system and cancer cells of the patient holds great promise for immunotherapy development [ 4 - 6 ]. (hindawi.com)
Researchers tested the tumors in three different mouse models of liver cancer to look for the presence of hybrid hepatocytes. (doctorshealthpress.com)
Studying a deadly type of breast cancer called triple negative, scientists say they have identified key molecular differences between cancer cells that cling to an initial tumor and those that venture off to form distant tumors. (sciencedaily.com)
The lethal nature of this type of cancer is marked by the fact that its cells lack molecular flags on its surface that connect with the hormones estrogen and progesterone and a cancer growth-promoting protein called Her2-neu. (sciencedaily.com)
More specifically, Ewald and Johns Hopkins postdoctoral fellow Eloïse Grasset, Ph.D., and others on the research team found several unique properties in the cells of mice engineered to have the mouse-version of triple negative breast cancers, and mice implanted with tumors from people with triple negative breast cancer. (sciencedaily.com)
To do this, breast cancer cells gain a cellular skeleton protein dubbed vimentin, which enhances the migratory ability of so-called mesenchymal cells, a cell type typically found in bones and bone marrow that moves around and makes new cells. (sciencedaily.com)
Triple negative breast cancer cells also gain survival advantages by producing a protein called E-cadherin, typically found in epithelial cells that line the ducts and coverings of organs and frequently renew themselves. (sciencedaily.com)
When triple negative breast cancer cells gain such survival and migratory qualities, scientists classify their cellular state as so-called hybrid epithelial mesenchymal (EMT) cells. (sciencedaily.com)
This paper develops a three-dimensional in silico hybrid model of cancer, which describes the multi-variate phenotypic behaviour of tumour and host cells. (neurosciencenews.com)
I wrote this because, at the time, I was getting tired of being asked about whether or not cell phones caused cancer. (gatech.edu)
Only certain types of radiation are capable of causing chemical reactions, and hence, a cancer cell. (gatech.edu)
The tiny Philadelphia chromosome became a clear and consistent marker of CML, a cancer of the myeloid or bone marrow cells, with broad implications for diagnosis and prognosis of disease. (laskerfoundation.org)
Cancer has been in existence on Earth even before the appearance of man, as evidenced by paleontological findings of tumors in animals ( 1 , 2 ). (jcancer.org)
LINE-1 are poorly expressed in normal cells and abundantly in cancer cells. (unife.it)
Decreasing RT activity in cancer cells, by either LINE-1-specific RNA interference, or by RT inhibitory drugs, was previously found to reduce proliferation and promote differentiation and to antagonize tumor growth in animal models. (unife.it)
In cancer cells the abundant RT reverse-transcribes retroelement-derived mRNAs forming RNA:DNA hybrids. (unife.it)
Common examples include cancer tumorspheres, which enable the study of oxygen gradients and nutrient access in tumor formation. (sigmaaldrich.com)
We envisaged Pt having a dual function: to power an NDDS (electroporation capability) as an active nanoparticle catalyst, and as Pt 2 +, to kill cancer and microbial cells with cytotoxicity properties. (scielo.org.za)
Currently, cancer treatment uses electroporation where the power source is applied through the skin (invasive) to destroy the affected tumour cells and to allow drug entry from the vascular system into the cell through pores induced into the cell membranes. (scielo.org.za)
arations verified the presence of cancer cells in all samples. (lu.se)

In order to investigate in detail how the avian homologue ITA interfered with both TNF induced apoptosis and the NGF mediated differentiation in PC12 cells, a two hybrid screen was performed with a PC12 library using ITA as a bait. (uni-wuerzburg.de)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)

The tumor was considered free of node involvement and metastases. (cdc.gov)
Verrucous carcinoma of both the skin and the mucosa is an uncommon low-grade squamous cell carcinoma that is clinically evident as a slowly but relentlessly enlarging warty tumor, histologically characterized by local invasion with minimal dysplasia, if any, and biologically characterized by a low incidence of metastases. (medscape.com)
1] Even when the tumor is large, when it has been present for many years, and when it penetrates the bone, distant metastases are rare. (medscape.com)

In addition, the isolation and storage of umbilical cord blood hematopoietic stem cells have been used to rebuild the immune system for the treatment of hematological malignancies and might be promising for the dynamic equilibrium expansion of functional T cells [ 12 ]. (hindawi.com)
they are used to treat solid and hematopoietic tumors, inflammatory disorders, and infections. (msdmanuals.com)

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. (nature.com)
Chronic viral infections in the liver can lead to organ dysfunction and ultimately to liver tumors in a progression invariably characterized by viruses that proliferate free of immune system restraints. (medicalxpress.com)
When safe and gross cytoreduction is achieved, residual infiltrating GBM cells undetectable by advanced imaging techniques still remain in the peritumoral area leading to disease progression and inevitable patient's death ( 7 , 8 ). (frontiersin.org)
The model is tailored to simulate glioma progression, and is subsequently used to interrogate the balance between the host cells and small sized gliomas, while the go-or-grow phenotype characteristic in glioblastomas is also investigated. (neurosciencenews.com)
Pancreatic tumor-associated macrophages are considered M2 macrophages and are thought to support tumor progression through the engagement of multiple mechanisms that promote immune tolerance. (aacrjournals.org)

These results shed light on the mechanisms that underlie the emergence of different levels of infiltration of T cells into the tumour and elucidate how T-cell infiltration shapes anti-tumour immune response. (hal.science)
In this study, we compared DC-tumor cell hybrids with the non-fused mixture of DC and tumor cells directly in their ability to induce a specific immune response. (ox.ac.uk)
In vivo, A3@GMH efficiently induced ICD effects with two rounds of PTT, activated the host's antitumor immune response, and effectively suppressed tumor growth in 4T1 tumor-bearing mice, achieving an 88.9% tumor inhibition rate with no apparent toxic side effects. (bvsalud.org)

CTCs-derived organoids (CTCDOs) were characterized through proteome profiling, immunohistochemistry, immunofluorescence, flow cytometry, tumor-forming capacity and drug screening assays. (unicatt.it)
Cell phenotype was evaluated by flow cytometry and antigen-presenting ability by co-culture with syngeneic T cells followed by tetramer analysis and interferon (IFN)-γ ELISPOT. (ox.ac.uk)
The targeting properties of A3@GMH on 4T1 cells were assessed using a high-content imaging system and flow cytometry. (bvsalud.org)

Here, we present an integrated workflow for quantitative in vivo cell-cycle profiling. (nature.com)
Analysis of mitosis and antimitotic drug responses in tumors by in vivo microscopy and single-cell pharmacodynamics. (nature.com)
Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo . (nature.com)
Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)
Though these manufactured supports are less like the in vivo ECM, they may enhance reproducibility and facilitate cell retrieval from the culture. (sigmaaldrich.com)

CAR T cell immunotherapy for solid tumors? (medicalxpress.com)
It is noted that T cell has great potential for immunotherapy of hematological malignancies. (hindawi.com)

prise about 4% of cancers in women, invasive squamous-cell carcinoma. (who.int)

It should be noted that while the study was principally performed using mice models, researchers have found cells similar to the mouse hybrid hepatocytes in human livers. (doctorshealthpress.com)

For example, immunoglobin-producing B lymphocytes can be fused with myeloma to produce an immortal line of cells called hybridoma that secrete immunoglobin. (wikipedia.org)
Background TCR repertoire profiling can provide a useful window into the complex interactions between tumor cells and infiltrating lymphocytes. (bmj.com)

We report a case of chronic Schistosoma haematobium infection with pseudometastatic pulmonary nodules and high-grade squamous cell carcinoma in a 30-year-old man in Mali. (cdc.gov)
Schistosomiasis-induced squamous cell carcinoma of the bladder with pseudometastatic pulmonary nodules in a 30-year-old man from Mali. (cdc.gov)
Anatomopathology revealed urinary schistosomiasis complicated by a high-grade, well-differentiated, keratinized squamous cell carcinoma (SCC) (Figure, panel B). Within the wall, ovoid structures, sometimes calcified, corresponded to bilharzia eggs. (cdc.gov)
The typical microscopic section shows a well-differentiated typical squamous cell carcinoma with verrucous clinical morphology. (medscape.com)
Oral verrucous carcinoma should be recognized as an important clinicopathologic variant of squamous cell carcinoma. (medscape.com)
To those unfamiliar with oral verrucous carcinoma, its morphologic warty or verrucous appearance and its relatively bland histologic features often suggest verruca vulgaris or pseudoepitheliomatous hyperplasia rather than squamous cell carcinoma. (medscape.com)

Our data indicate that the generation of THCs depends on the expression of CD36 on tumor stem cells and the oxidative state and polarization of monocytes, the latter being strongly influenced by microenvironmental fluctuations. (uam.es)
Highly oxidized M2-like monocytes show the strongest affinity to fuse with tumor stem cells. (uam.es)
The study's first author, Joan Font-Burgada, notes that "Although hybrid hepatocytes are not stem cells, thus far they seem to be the most effective in rescuing a diseased liver from complete failure. (doctorshealthpress.com)
Currently, induced pluripotent stem cells (iPSCs) are being tested for the reparation of damaged livers and the treatment of liver failure. (doctorshealthpress.com)
Glioma is a broad category of brain and spinal cord tumours that originate from neural stem cells and glial cells that support neurons. (neurosciencenews.com)

Conclusions By incorporating routine TCR repertoire profiling into a high-volume clinical genomic sequencing program, we have developed a rich, multi-modal resource for studying the complex tumor-immune interaction. (bmj.com)
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. (sigmaaldrich.com)
Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)

This dataset is a valuable resource for TCR therapeutic discovery, and can help identify naturally occuring TCRs that may minimize on-target, off-tumor toxicity. (bmj.com)

Another well-documented role of M2 macrophages is the remodeling of the tumor stroma via collagen production and degradation, with the latter being mediated by mannose receptor (Mrc1)-mediated endocytosis of collagen. (aacrjournals.org)
Given our previous work showing that protein scavenging by tumor cells can be utilized as a nutrient delivery mechanism, we set out to investigate the functional significance of collagen scavenging by macrophages. (aacrjournals.org)
Our findings suggest a new modality for the functional modulation of tumor-associated macrophages through the coupling of extracellular matrix remodeling to protein scavenging. (aacrjournals.org)

METHODS: Hybrids were obtained by electrofusion of tumor cells and monocyte-derived DCs. (ox.ac.uk)

Results: CTCDOs exhibited a hybrid epithelial-mesenchymal transition (EMT) state and an increased expression of stemness-associated markers including the two homeobox transcription factors Goosecoid and Pancreatic Duodenal Homeobox Gene-1 (PDX1), which were also detected in CTCs from CRC patients. (unicatt.it)
In fact, cultures derived from patients' CTCs usually take many months to grow (which is incompatible with their use for treatment decision) and may be scarcely representative of tumor complexity [ 28 ]. (biomedcentral.com)

T cell immune protection plays a pivotal role in the treatment of patients with hematological malignancies. (hindawi.com)
Since 2015, a medical device based on tumor-treating (TT)-Fields can also be applied on supratentorial GBM patients. (frontiersin.org)
In this study, we analyzed a large, real-world, clinicogenomic database of over 130k patients - with T-cell repertoire data covering a diverse landscape of HLA genotypes and tumor neoantigens - to identify public TCRs associated with HLA-specific neoantigens and viral epitopes. (bmj.com)
I came to realize that there were many questions about chromosome changes in patients that would be rewarding to study," noted Rowley, and for the next decade she labored over the microscope looking at chromosomes in leukemic cells. (laskerfoundation.org)

Moreover, the clonogenic assay showed that the radiosensitivity of MG63 cells was increased by the combination use of CSAF NPs and 5-ALA. This was supported by performing a western blot that confirmed the expression of cytochrome c (a marker of cell mitochondria damage) and caspase-3 (a marker of cell apoptosis). (tmu.edu.tw)
For anyone who has taken Biology 101, the concept of apoptosis-programmed cell death-is taught early in the course. (medicalxpress.com)
One method is to target senescent T cells which have selective apoptosis function. (hindawi.com)
The widely studied 32D cell system was chosen to investigate the effect of NRAGE on apoptosis. (uni-wuerzburg.de)
Thus NRAGE was identified during this research doctorate as a novel pro-apoptotic, IAP-interacting protein, able to accelerate apoptosis in a pathway independent of Bcl-2 cell protection. (uni-wuerzburg.de)

Bamets were able to overcome cisplatin resistance in all cell lines. (aspetjournals.org)

Adoptive cell therapy (ACT) with chimeric antigen receptor T (CAR-T) cells can restore the activity of exhausted T cell through reprogramming and is widely used in the treatment of relapsed/refractory (r/r) hematological malignancies. (hindawi.com)
Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)

It combines image analysis and machine learning methods for automated 3D segmentation and cell-cycle state identification of individual cell-nuclei with widely varying morphologies embedded in complex tumor environments. (nature.com)
The proposed in silico hybrid modelling framework combines successfully the advantages of continuum-based and discrete methods, namely the finite element and agent-based method respectively. (neurosciencenews.com)
The interactions observed in yeast cells were confirmed in mammalian cell culture, employing both coimmunoprecipitation and mammalian two-hybrid methods. (uni-wuerzburg.de)
3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)

Neuroblastoma tumors are derived from the sympathetic nervous system and exhibit neural crest features. (lu.se)

This includes the development of novel proteomics methodologies, identification of liquid biopsy signatures and the molecular identification of novel cell surface markers. (stanford.edu)

Participation of the H-2 antigens of tumor cells in their lysis by syngeneic T cells. (rupress.org)

The most dominant techniques are PCR viral detection and signal amplification DNA-based assays (Hybrid Capture II is the only FDA-approved technique at present). (medscape.com)

Various biological processes including immunoreaction of infection, tumor prevention, and human aging could cause telomere damage, tumor-related stress responses, and T regulatory (Treg) cells activation, which even trigger T cell senescence showing the distinctive phenotypic and functional alternation [ 9 , 10 ]. (hindawi.com)

We hypothesize that, in the future, the hybrid-catalyst assembly will be made using the membrane electrode assembly (MEA) with layered nano-particles and an enzyme hybrid assembly. (scielo.org.za)

Phosphorylation at CDK1, CDK4, and CDK9 sites on, respectively, protein phosphatase 1alpha, retinoblastoma protein, and RNA polymerase II is inhibited in neuroblastoma SH-SY5Y cells exposed to meriolins. (rcsb.org)
In differentiating neuroblastoma cells, HASH-1 is down-regulated, and there is coincident up-regulation of the transcriptional repressor HES-1, which is known to bind the HASH-1 promoter. (lu.se)

In RAW 264.7 cells, flavopiridol treatment inhibited IL-1β, IL-6 and TNF-a expression but promoted IL-10 expression. (researchsquare.com)
Flavopiridol treatment notably upregulated the protein expression of LC3, Beclin-1 and Atg7 in infected corneas as well as in RAW 264.7 cells. (researchsquare.com)
The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
The expression of MDR1 and GST-P was only enhanced in LS 174T/R. A higher expression of p53 was seen in LS 174T/R and Hepa 1-6/R cell lines but not in WIF-B9/R cells. (aspetjournals.org)
Interestingly, NRAGE was able to overcome the protection afforded to 32D cells by the exogenous expression of human Bcl-2. (uni-wuerzburg.de)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. (nature.com)

Dendritic-tumor cell hybrids induce tumor-specific immune responses more effectively than the simple mixture of dendritic and tumor cells. (ox.ac.uk)

The model encompasses the role of cell migration and adhesion, the influence of the extracellular matrix, the effects of oxygen and nutrient availability, and the signalling triggered by chemical cues and growth factors. (neurosciencenews.com)

The team discovered this new group of cells, called hybrid hepatocytes , in the portal triad of the liver. (doctorshealthpress.com)
Study authors thereby concluded that the cells did not contribute to the formation of cancerous liver tumors. (doctorshealthpress.com)
The study's lead author, Michael Karin, suggests, "Hybrid hepatocytes represent not only the most effective way to repair a diseased liver, but also the safest way to prevent fatal liver failure by cell transplantation. (doctorshealthpress.com)

http://www.cell.com/cell/abstract/S0092-8674%2815%2900903-4 , 162(4): 766-779, doi: 10.1016/j.cell.2015.07.026. (doctorshealthpress.com)

Removing senescent T cells from the physiological cycle to maintain the homeostasis of memory and effector T cells is the way of replacement. (hindawi.com)

Microarrays were prepared in tumor biology. (lu.se)

Some oncogenic viruses that code for fusogens and are capable of hybridizing cells are: Epstein-Barr virus HPV Hepatitis B and Hepatitis C Human T-cell lymphotropic virus type 1 (HTLV-1) The virus used in laboratories for controlled cell hybridization is inactivated Sendai virus. (wikipedia.org)
Human-mouse hybrid cells, i.e. (wikipedia.org)
Humsters: a hybrid of human sperm and hamster eggs. (wikipedia.org)
These are created as a byproduct of fertility testing, when human sperm potency is measured by how well it can penetrate a hamster egg cell (hamster eggs tend to have similar penetrability to human sperm as do human eggs). (wikipedia.org)
However, from the beginning of puberty after thymus degeneration, human cytomegalovirus (HCMV) persists due to the chronic activation of cytomegalovirus in humans causing repeated activation of T cells, which is considered the driving factor of human immune aging [ 9 ]. (hindawi.com)
We have investigated the sensitivity of the cisplatin-resistant enterohepatic tumor cell lines LS174T/R (human colon adenocarcinoma), WIF-B9/R (rat hepatoma-human fibroblast hybrid), and Hepa 1-6/R (mouse hepatoma) to free and liposome-encapsulated cytostatic bile acid derivatives Bamet-R2 and bamet-UD2. (aspetjournals.org)
These findings suggest that cell fusion might have induced epigenetic SCE frequency changes possibly in the same way as modulation of SCE frequencies is known to occur in the human leukocyte series. (biologists.com)
NRAGE also interacted with the human XIAP in direct two-hybrid tests. (uni-wuerzburg.de)
Amplified DNA from cells harboring human papillomavirus are used as positive controls. (medscape.com)

Lattice-gas Cellular Automaton Modelling of Developing Cell Systems. (maa.org)
Center-based Single-cell Models: An Approach to Multi-cellular Organization Based on a Conceptual Analogy to Colloidal Particles. (maa.org)

The TrueGel3D® HTS Hydrogel Plate is a ready-to-use solution to easily establish 3D cell cultures using fully synthetic hydrogels in a simple and automation-compatible manner. (sigmaaldrich.com)

Two observations directly demonstrated the participation of the H-2 antigens on the tumor cells in their lysis by H-2-compatible T cells. (rupress.org)
Specific antisera reacting with these H-2 antigens, but not those of the target cell, failed to inhibit lysis when small numbers of effector cells were assayed against H-2-incompatible target cells or when effector cells of F1-hybrid origin and bearing two H-2 haplotypes were assayed against a tumor cell of one of the parental strains. (rupress.org)

Anatomy42

Organisms13

Diseases62

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes75

Disciplines and Occupations2

Information Science3

Health Care2

Microenvironment2

Malignant6

Chemotherapy5

Tissue16

Tissues9

Suppressor1

Nanoparticles5

Radiotherapy3

Vitro9

Metastatic10

Cytotoxic7

Genes9

Achieve tumor1

Resection2

Proteins5

Solid tumor2

Anti-tumor2

Brain tumor1

Therapies4

Characterization2

Xenograft2

Metastasis4

Malignancy1

Molecules6

Circulating1

Cancer32

Differentiation2

Metastases3

Hematopoietic2

Progression5

Immune response3

Flow cytometry3

Vivo5

Immunotherapy2

Invasive1

Mice1

Lymphocytes2

Squamous cell carc6

Stem cells5

Interaction3

Toxicity1

Macrophages3

Electrofusion1

CTCs2

Patients4

Apoptosis5

Resistance1

Antigen5

Methods4

Sympathetic nervou1

Molecular1

Syngeneic1

Assays1

Phenotypic1

Membrane1

Neuroblastoma2

Expression7

Mammalian cell culture1

Phenotype1

Effectively1

Extracellular1

Liver3

10.10161

Effector T cel1

Biology1

Human9

Cellular2

Cultures1

Lysis2