Tumor cell cells in vitro. Medical search. Frequent questions

In vitro and in vivo anti-tumor activity of CoQ 0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis. (oncotarget.com)
This study examined the in vitro or in vivo anti-tumor, apoptosis, and anti-metastasis activities of CoQ 0 (0-20 μM) through inhibition of Wnt/β-catenin signaling pathway. (oncotarget.com)
Histological analysis and western blotting confirmed that CoQ 0 significantly decreased the xenografted tumor progression as demonstrated by induction of apoptosis, suppression of β-catenin, and inhibition of cell cycle-, apoptotic-, and metastatic-regulatory proteins. (oncotarget.com)
Apoptosis was determined by cell death assay and western blotting. (biomedcentral.com)
Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)
Triphala-induced apoptosis was linked with phosphorylation of p53 at Ser-15 and ERK at Thr-202/Tyr-204 in Capan-2 cells. (biomedcentral.com)
Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)
On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)
The results showed that chrysin inhibited cancer cell growth at a dose-dependent manner by inducing apoptosis and cell cycle arrest at G2/M phase. (propolisscience.org)
Moreover, the clonogenic assay showed that the radiosensitivity of MG63 cells was increased by the combination use of CSAF NPs and 5-ALA. This was supported by performing a western blot that confirmed the expression of cytochrome c (a marker of cell mitochondria damage) and caspase-3 (a marker of cell apoptosis). (tmu.edu.tw)
Transcriptomics analyses showed pathways related to TCR signaling, cytotoxicity and oxidative phosphorylation were significantly enriched in Tet lo found in both regressing and progressing tumors compared with Tet hi , whereas genes related to DNA damage, apoptosis and autophagy were downregulated. (bmj.com)
Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)
Positively charged CTAC could actively target mitochondria by interacting with the negatively charged mitochondria membrane, and then lead to the dysfunction of mitochondria by decreasing mitochondrial potential and intracellular ATP levels, resulting in the necrosis and apoptosis of MCF-7 cells. (rsc.org)
It initiates the process of programmed cell death, or apoptosis, which directs the cell to commit suicide, permanently removing the damaged DNA from the organism. (news-medical.net)
Instead of accumulating in mouse cells and halting cell division in the genetically engineered mice, the altered p53 protein performed flawlessly: it was unstable when no DNA damage was present and was stable and fully functional when needed to halt the cycle cell to repair DNA damage or to induce apoptosis. (news-medical.net)

Further, oral administration of 50 mg/kg or 100 mg/kg Triphala in PBS, 5 days/week significantly suppressed the growth of Capan-2 pancreatic tumor-xenograft. (biomedcentral.com)
Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)
In mouse and rat xenograft tumor models, TAK-285 showed antitumor activity against cancers that expressed HER2 or EGFR. (jcancer.org)
In murine melanoma xenograft models, this bispecific antibody was shown to significantly inhibit tumor growth. (mskcc.org)
The reduction in tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable to those produced by 5-FU. (spandidos-publications.com)
The STAT3 Inhibitor Galiellalactone Effectively Reduces Tumor Growth and Metastatic Spread in an Orthotopic Xenograft Mouse Model of Prostate Cancer. (lu.se)

Furthermore, systemic treatment of tumor-bearing mice with chlorambucil-CREKA-pVEC significantly reduced tumor growth compared to control groups. (avhandlingar.se)

Aggregation of phagocytic macrophages within clusters and the nonlinear dependence of the cluster growth rate on macrophage number suggest that macrophages may exhibit cooperativity in engulfing solid tumor targets, which resembles the foreign body response to biomaterials in some ways. (aiche.org)
We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)
Furthermore, the in vivo study results revealed that CoQ 0 treatment inhibited the tumor growth in B16F10 xenografted nude mice. (oncotarget.com)
TNF-alpha generally inhibits the growth of primitive murine hematopoietic progenitor cells (Lin-Scal+) in response to multiple cytokine combinations, and the p75 TNF receptor is essential in signaling such inhibition. (ox.ac.uk)
In contrast, TGF-beta 1, another bifunctional regulator of hematopoietic progenitor cell growth, completely blocked SCF plus IL-7-induced proliferation. (ox.ac.uk)
Growth-inhibitory effects of Triphala were evaluated in Capan-2, BxPC-3 and HPDE-6 cells by Sulphoradamine-B assay. (biomedcentral.com)
Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)
Recently, researches on this field point out the idea that IL-17 promotes tumor growth, especially through the activation of IL-6- STAT3 cascade, an oncogenic transcription factor that up regulates pro-survival and pro-angiogenic genes [1-4]. (austinpublishinggroup.com)
For bladder cancer, the tumor was reduced in IL-17 -/- mice, indicating a role of this cytokine in promoting tumor growth through IL-6/ STAT3 pathway [4]. (austinpublishinggroup.com)
In vitro study has been conducted by using MCF-7 cell lines treated with external electrostatic for 24, 48, and 72 hours of treatment and the cells number were calculated during treatment by using hemocytometer and presented as Growth Inhibition (GI)% efficacy. (uai.ac.id)
Moreover, the external electrostatic caused 28-39% growth inhibition efficacy of MCF-7 cells. (uai.ac.id)
Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study. (propolisscience.org)
Moreover, chrysin suppressed melanoma tumor growth at an average of 60% (after 14 days of treatment) and 71% (after 21 days of treatment) compared to the tumor-bearing group. (propolisscience.org)
TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
It is an alkylating agent, and its mechanism of action of active metabolites may involve cross-linking of DNA, which may interfere with growth of normal and neoplastic cells. (medscape.com)
these 2 events, in turn, can inhibit growth of neoplastic cells. (medscape.com)
Reversible proteasome inhibition disrupts pathways supporting cell growth, thus decreasing cancer cell survival. (medscape.com)
Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
The vascular endothelial growth factor (VEGF) is a well characterized angiogenic factor known to stimulate angiogenesis within a tumor ( 1 ). (spandidos-publications.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
Further, GA-mPEG 2000 micelles were effective in inhibiting tumor growth and prolonged survival in subcutaneous B16-F10 and C26 tumor models. (dovepress.com)
Moreover, in vivo studies confirm that the CTAC@MSNs-HSA are able to induce cancer cell death and efficiently inhibit tumor growth. (rsc.org)
It has also been shown that metformin and phenformin inhibit tumor growth by disrupting mitochondrial complex. (rsc.org)
If p53 is inactivated, as it is in over half of all human cancers, checks and balances on cell growth fail to operate, and body cells start to accumulate mutations, which ultimately may lead to cancer. (news-medical.net)
Cell growth and viability were assessed after 7 and 10 days. (bvsalud.org)

Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)
Infiltration versus metastasis is one of the most characteristic biological properties of tumors [ 5 ]. (hindawi.com)
Generally, it is believed that there are four main pathways between tumor invasion and metastasis, including local invasion, blood, lymphatic, and seeding metastasis [ 6 ]. (hindawi.com)
In addition to these four pathways, tumors can undergo infiltration around nerve fibers and metastasis along nerves, that is, perineural invasion (PNI), which refers to the phenomenon of perineural invasion by tumor cells filling the perineurial space, wrapping around nerves in a continuous concentric sheath-like pattern, infiltration, and metastasis of extension along nerves around nerve fibers or into perineurium within the perineurium [ 7 ]. (hindawi.com)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
Angiogenesis is essential in the process of carcinogenesis to facilitate tumor progression and metastasis. (spandidos-publications.com)
Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
1 It has become increasingly evident that mitochondria play a key role in the generation, proliferation and metastasis of cancer cells. (rsc.org)

Notably, non- or sub-cytotoxic concentrations of CoQ 0 markedly inhibited migration and invasion, accompanied by the down-regulation of MMP-2 and -9, and up-regulation of TIMP-1 and -2 expressions in highly metastatic B16F10 cells. (oncotarget.com)
SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
Many of these cell lines have been obtained from a highly metastatic malignant tumor of the trophoblast, the choriocarcinoma, and have been extensively characterized. (biomedicineandprevention.com)
We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)
Chicago, IL (June 4, 2011) -- Data presented at the 47th American Society of Clinical Oncology (ASCO) annual meeting demonstrate the potential of circulating tumor cells (CTC) as a biomarker for metastatic prostate cancer clinical trials. (jnj.com)
Results of the study titled, " Evaluation of circulating tumor cell (CTC) enumeration as an efficacy response biomarker of overall survival (OS) in metastatic castration resistant prostate cancer (mCRPC) " indicated that pre-treatment, CTCs and lactate dehydrogenase (LDH), both alone and in combination, were prognostic biomarkers, while baseline prostate-specific antigen (PSA) was not. (jnj.com)
An independent study investigating the in vitro diagnostic use of CTC testing found that the number of CTCs in the blood may help physicians more reliably assess treatment benefit for patients with metastatic breast cancer. (jnj.com)
Cancer stem cells are responsible for tumor chemoresistance and recurrence in adjuvant and metastatic settings. (isciii.es)

This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
Apoferritin-encapsulation of cysteine protease inhibitors for cathepsin L inhibition in cancer cells. (nottingham.ac.uk)

Tumor necrosis factor-alpha (TNF-alpha) potently enhances in vitro macrophage production from primitive murine hematopoietic progenitor cells in combination with stem cell factor and interleukin-7: novel stimulatory role of p55 TNF receptors. (ox.ac.uk)
Tumor necrosis factor-alpha (TNF-alpha) is a bifunctional regulator of hematopoiesis, and its cellular responses are mediated by two distinct cell surface receptors. (ox.ac.uk)
In tumor necrosis factor (TNF)-alpha-stimulated human aortic endothelial cells, beta-sitosterol was found to significantly inhibit vascular adhesion molecule 1 and intracellular adhesion molecule 1 expression. (drugs.com)
Tumour necrosis factor (TNF)-α is thought to be a key early cytokine in the pathogenesis of sarcoidosis, despite conflicting data. (ersjournals.com)
This study has demonstrated that a subset of mononuclear phagocytes, mature macrophages, are responsible for tumour necrosis factor secretion and this could have implications for targeted management in sarcoidosis in the future. (ersjournals.com)
Tumour necrosis factor (TNF)-α is a key early cytokine in a variety of chronic inflammatory responses, including fibrosing alveolitis and granulomatous disease 6 - 8 . (ersjournals.com)

Anti-tumor IgG administered intravenously to mice eliminated a fraction of CD47 knockout tumors in vivo but had no effect on wild-type tumors. (aiche.org)
Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice with mammary tumors. (nature.com)
The mice were injected with either MCF-7 cells, mammary tumor cells from C3H donor, or NaCl 0.9% (placebo) subcutaneously into the axilla area and exposed by external electrostatic in each cage for 12 hours in 2 weeks before necropsied. (uai.ac.id)
After 2 weeks of exposure, placebo mice were physically normal, whereas the tumor undergone significant shrinkage of more than 67% in size. (uai.ac.id)
The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice. (propolisscience.org)
In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b.w) for 14 days and 21 days. (propolisscience.org)
Subsequently, we used a model of neuroinvasion in nude mice to assess the effect of NGF in vivo on tumor nerve invasion as well as on nociceptive transmission. (hindawi.com)
Genome-scale mutational signatures of aflatoxin in cells, mice, and human tumors. (who.int)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Cell migration capacity and intracellular signaling pathways activated by IL-17 were assessed by wound healing assay and flow cytometry, respectively. (austinpublishinggroup.com)
Our observations suggest that the tumor paracrine effect affects biological pathways of pleurodesis. (medscimonit.com)
P-Tex cells could aggregate in the antigen-presenting cell niches and activate certain signaling pathways. (elifesciences.org)

Breast cancer is one of the most common primary tumors to develop associated brain metastases ( 1 , 2 ), and while recent progress in cancer therapy has improved control of primary breast tumors, methods to effectively treat brain metastases from breast cancer remain inadequate. (jcancer.org)

However, the incubation of IL-17 was able to promote tumor cell migration and AKT, ERK and P38 phosphorylation in vitro . (austinpublishinggroup.com)

Solid tumors pose significant challenges for immunotherapies including those employing phagocytic macrophages as effector cells. (aiche.org)
Macrophages and giant cells composed of macrophages are abundant in many neoplasms, but it is unknown whether they can phagocytose target cancer cells that adhere to one another or whether phagocytosis can outcompete proliferation. (aiche.org)
We show that macrophages can eliminate melanoma cells in vitro from rapidly proliferating clusters formed on non-adhesive materials. (aiche.org)
Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)
In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
Histopathological analysis of the mammary glands indicated infiltration of macrophages into the tumor area through the blood vessel. (uai.ac.id)
A greater number of alveolar macrophages produced TNF-α compared to autologous monocytes in sarcoidosis but not in controls and, based on cell size, it was confirmed that this was the product of more mature macrophages and that the secretion of TNF-α by monocytes and macrophages was heterogeneous: not all monocytes and macrophages secrete TNF-α. (ersjournals.com)

Thus, our in vitro and in vivo models utilizing gene-edited cells, monoclonal antibodies for opsonization and checkpoint blockade, and cell therapy begin to define the parameters required for successful immunotherapy of solid tumors including (i) macrophage density, (ii) inhibitory checkpoint disruption, and (iii) IgG-opsonization. (aiche.org)

Micropipette aspiration rheology of cell clusters and freshly isolated mouse tumors revealed viscoelasticity and cell cohesion in both tissues. (aiche.org)
Very early during mammalian development the embryo differentiates into 2 main cell lineages, the inner cell mass (ICM) which will mainly contribute to embryonic tissues, and the trophoblast which will form extra-embryonic tissues. (biomedicineandprevention.com)
In this mini-review, we aim to present and critically analyse the different in vitro models that have been proposed as an alternative to in vivo and ex vivo studies to elucidate the ability of different xenobiotics to interfere with fetal development by direct (transplacental crossing and accumulation in embryonic tissues) or indirect action (e.g., inflammation, perturbation of placental function). (biomedicineandprevention.com)
Western blotting was employed to determine NGF level in PC and paracarcinoma tissues and in PC cell lines as well as pancreatic ductal epithelial cells. (hindawi.com)
NGF level was preeminently higher in PC tissues and cell lines than in paracarcinoma tissues and normal pancreatic epithelial cell lines. (hindawi.com)
The tumor size and the number of proliferating cells were reduced in the tumor tissues obtained from the formononetin-treated group. (spandidos-publications.com)

Although carcinosarcoma is the preferred term for this group of tumors, according to the International Society of Gynecological Pathologists (ISGyP)/World Health Organization (WHO) classification, it is also referred to as malignant mixed müllerian tumor. (medscape.com)
Both epithelial and mesenchymal components of carcinosarcoma show high-grade malignant cells. (medscape.com)
Malignant cells might have an impact on the mesothelial response to applied sclerosing agents and, in consequence, on the efficacy of pleurodesis. (medscimonit.com)
We take advantage of the similarity between neural developmental processes and cancer hierarchy to mitigate, if not completely abolish, the malignant nature of tumor cells and pave the way for new intervention strategies. (lu.se)
PC-ALCL is one of the primary cutaneous CD30 + T-cell lymphoproliferative disorders, a wide spectrum of disease, with lymphomatoid papulosis (LyP) at the benign end of the spectrum and PC-ALCL at the malignant end. (medscape.com)
First described in 1848, MM is characterized by a proliferation of malignant plasma cells and a subsequent overabundance of monoclonal paraprotein (M protein). (medscape.com)

SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
1997. In vitro genotoxic effects of hard metal particles assessed by alkaline single cell gel and elution assays. (cdc.gov)
MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
Tetramer staining was performed to determine the frequency and avidity of CD8 + T cells targeting the tumor-specific epitope GSW11 and confirmed with tetramer competition assays. (bmj.com)
In vitro cytotoxicity assays and in vivo adoptive transfer experiments were performed to determine the cytotoxicity of high and low avidity populations. (bmj.com)

Another study suggests that IL-17 may play an important role in the recruitment and infiltration of antitumor immune cells in early stages of bladder cancer [5]. (austinpublishinggroup.com)
TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-{alpha]}-mediated antitumor reactivity. (invivogen.com)
Therefore, significant antitumor activity is observed by in vitro studies. (rsc.org)

The present study aimed to clarify the direct action of IL-17 in a series of solid tumor human cells, from glioma, esophageal carcinoma and bladder cancer. (austinpublishinggroup.com)

Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)

In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)

It is within the cell-autonomous context that ST6GAL1 involvement in cancer progression has been interpreted. (nature.com)
Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)

The capability of cell-penetrating peptides (CPPs) to transport cargos over different cellular membranes both in vitro and in vivo have drawn major attention in the past decade. (avhandlingar.se)
The translocation of CPPs into plant protoplasts might open up a new method for transformation of plant cells.Next, the cell-penetrating ability of the novel peptide YTA2 was characterized and it was established that chemical coupling between YTA2 and the protein cargo is not needed for the transport of the cargo over the cellular membrane in vitro. (avhandlingar.se)
Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
The proteasome pathway is an enzyme complex existing in all cells, which degrades ubiquitinated proteins that control the cell cycle and cellular processes and maintains cellular homeostasis. (medscape.com)
Mitochondria play an important role in supplying cellular energy, cell signaling and governing cell death. (rsc.org)
The cellular cascade of molecular signals that instructs cells with fatally damaged DNA to self-destruct pivots on the p53 tumor suppressor gene. (news-medical.net)
Since the p53 protein is able to trigger such drastic action as cellular suicide, the cells of the body must ensure that the p53 protein is only activated when damage is sensed and that the protein is quickly degraded when it is not needed. (news-medical.net)

CSPG4 is an established melanoma-associated tumor antigen (also known as High-molecular-weight melanoma-associated antigen). (mskcc.org)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)

The absorbances averages results obtained showed that the crude extract did not present cytotoxicity for the HTC cells in all the concentrations and evaluated times. (scielo.br)
Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC). (scielo.br)
In vitro studies showed that Tet lo exhibits higher cytotoxicity than Tet hi . (bmj.com)
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) tests demonstrated that the GA-mPEG 2000 micelle formulation had obvious cytotoxicity to tumor cells and human umbilical vein endothelial cells. (dovepress.com)

After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)

The study used mesothelial cell (MeT-5A) cultures stimulated with sclerosing agents (talc, doxycycline, iodopovidone, and TGF-β for 24 h) in the presence or absence of supernatants from adenocarcinoma cultures (HCC827). (medscimonit.com)
Further, lung fibroblasts were cultured with and without cell supernatants from previously established cell cultures for 24 h. (medscimonit.com)
In this study conducted in partnership with the Institut Polytechnique de Paris and Inserm, the scientists created a 3D cancer cell culture which replicates tumor structure more effectively than traditional cultures. (pasteur.fr)

Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)

Methods CT26 models were treated with anti-PD-1 on days 3, 6 and 9 following subcutaneous tumor implantation generating variable responses during early tumor development. (bmj.com)

The cells were then treated with different concentrations of IL-17, ranging from 10 pg/mL to 100 ng/mL, and evaluated for IL-17 ability to modify tumor cell proliferation and viability through cell counting and MTT assay. (austinpublishinggroup.com)
Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. (bvsalud.org)

One vial containing 420 mg of trastuzumab, a humanised IgG1 monoclonal antibody produced by mammalian (Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)
Multiple myeloma (MM) is a debilitating malignancy that is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance ( MGUS ) to plasma cell leukemia. (medscape.com)

CoQ 0 exhibits a significant cytotoxic effect on melanoma cell lines (B16F10, B16F1, and A2058), while causing little toxicity toward normal (HaCaT) cells. (oncotarget.com)
In addition, IL-17 enhanced the cytotoxic effects of NK cells against tumor cells [6]. (austinpublishinggroup.com)
The study aim was to evaluate the cytotoxic activity, using the MTT test [3-(4,5-Dimethilthiazol-2-yl)-2,5-diphenil tetrazolium bromide], from the crude extract of Picrasma crenata (Pau Tenente) and its isolated compounds, quassin and parain, in culture of rat liver tumor cells (HTC). (scielo.br)
They then introduced cytotoxic T cells in proximity to the cancer cells and monitored their behavior by microscopy. (pasteur.fr)

The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
A new mouse model, created by scientists at the Salk Institute for Biological Studies , suggests that what researchers have learned about the regulation of p53 activity from in vitro studies may not be relevant to living, breathing organisms. (news-medical.net)
Many chemotherapeutical drugs used to treat cancer exert their biological effects on tumor cells through activation of the p53 pathway. (news-medical.net)

Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
Tight junctions between adjacent epithelial cells constitute the physiological barrier to fluid and macromolecules in epithelium. (nih.gov)
Using a physiologically relevant air-liquid interface exposure system, electrically tight monolayers of the human bronchial epithelial cell-line Calu-3 were exposed to fresh, whole mainstream cigarette smoke. (nih.gov)
In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)
Epithelial GPR35 protects from Citrobacter rodentium infection by preserving goblet cells and mucosal barrier integrity. (uni-ulm.de)

The reverse haemolytic plaque assay allows the measurement of cytokine production at a single cell level. (ersjournals.com)
The current authors have previously demonstrated, using a reverse haemolytic plaque assay (RHPA), that single-cell production of cytokine can be assayed 16 . (ersjournals.com)
This technique has an advantage over routine culture conditions, as the development of cytokine product by individual cells can be localised and measured, which therefore allows for the correlation of cytokine production with cell type. (ersjournals.com)

Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)

This approach seeks to model cancer immunotherapy in vitro using an innovative microfluidic system. (pasteur.fr)
Background CD8 + T cells are a highly diverse population of cells with distinct phenotypic functions that can influence immunotherapy outcomes. (bmj.com)
Further insights on the roles of CD8 + specificities and TCR avidity of naturally arising tumor-specific T cells, where both high and low avidity T cells recognizing the same peptide-major histocompatibility complex (pMHC) coexist in the same tumor, are crucial for understanding T cell exhaustion and resistance to PD-1 immunotherapy. (bmj.com)
Conclusions Targeting subdominant T cell responses with lower avidity against pMHC affinity neoepitopes showed potential for improving PD-1 immunotherapy. (bmj.com)

Pathologically, at an early stage, there is an influx of mononuclear cells followed by lymphocytes. (ersjournals.com)

In esophageal squamous cell carcinoma (ESCC), IL-17 led to the production of inflammatory chemokines (CXCL9, CXCL10 and CCL2, CCL20), which are associated with the migration of T cells, NK cells, and DCs, respectively. (austinpublishinggroup.com)
OE-21 human esophageal squamous cell carcinoma was a gift from INCA (Rio de Janeiro, Brazil). (austinpublishinggroup.com)
Rarely, mucinous differentiation or squamous cell carcinoma is seen. (medscape.com)
The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)

Both, PEGA-pVEC and CREKA-pVEC were taken up by different breast cancer cells in vitro. (avhandlingar.se)
These tumor-homing CPPs might improve both diagnosis and treatment of breast cancer tumors, by conjugation to therapeutic agents. (avhandlingar.se)
Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
90% of cancer-related deaths, is a multi-step process involving cancer cell intravasation into the bloodstream, dissemination to distant sites, and seeding in new organs 1 . (nature.com)
At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
We aimed to evaluate the impact of cancer cell paracrine on mesothelial cell response to different sclerosing agents. (medscimonit.com)
The compelling conclusion that proliferative exhausted T cells are associated with response in HPV+ head and neck cancer is supported by the cohort of 14 patients with paired tumor and adjacent normal tissue and rigorous bioinformatic analysis of nearly 50,000 single CD3+ T cell transcriptomes. (elifesciences.org)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
In recent years, we focused on investigating the anti-carcinogenic properties of total Astragalus saponins (AST) in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
Tripodal O-N-O Bis-Phenolato Amine Titanium(IV) Complexes Show High in vitro Anti-Cancer Activity EUROPEAN JOURNAL OF INORGANIC CHEMISTRY. (nottingham.ac.uk)
2 Mitochondria of cancer cells are different from those of normal cells in many ways. (rsc.org)
4 The mitochondrial membrane potential of cancer cells (∼−220 mV) is more negative than that of normal cells (∼−160 mV). 5 Therefore, mitochondria have been regarded as a novel target for cancer therapy. (rsc.org)
CTAB has been shown as a promising compound against head and neck cancer cells with minimal toxicity against normal fibroblasts, as well as a tumoricidal irritant in colorectal cancer surgery. (rsc.org)
The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
Recurrence and chemoresistance in pancreatic cancer are largely related to cancer stem cells. (isciii.es)
Although surgical resection can be applied when the tumor is well defined (20% of cases) 1 , most patients with pancreatic cancer are treated with chemotherapy. (isciii.es)
Unless promptly and properly repaired, these DNA breaks can let cell division spiral out of control, ultimately causing cancer. (news-medical.net)
Cancer Cell. (lu.se)
Galiellalactone Inhibits Stem Cell-Like ALDH-Positive Prostate Cancer Cells. (lu.se)
Hollstein M, Alexandrov LB, Wild CP, Ardin M, Zavadil J. Base changes in tumour DNA have the power to reveal the causes and evolution of cancer. (who.int)

In this study, osteosarcoma cells (MG63) and normal cells (MC3T3-E1) were used for an in vitro investigation, in which reactive oxygen species (ROS) assay, cell viability assay, clonogenic assay, and western blot were used to confirm the treatment efficiency. (tmu.edu.tw)
RIP assay was performed to validate NGF and miR-21 binding ability in MiaPaCa-2 cells. (hindawi.com)

CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)

Together, our findings suggest a promising role for P-Tex cells in the prognosis of patients with HPV-positive HNSCC by providing modest but persistent anti-tumor effects. (elifesciences.org)

The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)

The delivery of proteins into cells by mere coincubation with CPPs is an improvement, since the chemical coupling between the CPP and the cargo molecule, which adds time-consuming synthesis and purification steps, can be omitted.Finally, by conjugating each of the two breast tumor homing peptides, the cyclic cCPGPEGAGC (PEGA) or the linear CREKA peptide, to the CPP pVEC, two cell-penetrating peptides with homing properties were obtained. (avhandlingar.se)
When higher than normal levels of p53 tumor suppressor exist, there is enough p53 to bind to many regulatory sites in the cell's genome to activate the production of other proteins that will halt cell division if the DNA damage can be repaired. (news-medical.net)

These findings displayed that NGF/TrkA encouraged the neuroinvasive potential of PC cells by activating the Warburg effect in DRG cells through upregulation of TDE-miR-21-5p expression. (hindawi.com)
In vitro findings indicate that MSK's novel construct effectively retargets T-cells to CSPG4 (+) tumor cells. (mskcc.org)
Our findings suggest that GA-mPEG 2000 micelles may have promising applications in tumor therapy. (dovepress.com)

IL-17RA mRNA expression was detected on the cell lines tested, but the incubation of IL-17 did not alter tumor cell viability or proliferation. (austinpublishinggroup.com)
The cell viability ratio of normal cells to tumor cells after treatment with CSAF NPs and 5-ALA reached 2.79. (tmu.edu.tw)
001). Cell viability in all the groups was over 80%, except in group IV at day 7. (bvsalud.org)

Adoptive transfer of Tet lo showed more effective tumor control than Tet hi , and curative responses were achieved when Tet lo was combined with two doses of anti-PD-1. (bmj.com)

Mesothelial cells are considered to play a pivotal role in the response to different chemical compounds (sclerosants) used for pleurodesis. (medscimonit.com)

In parallel, as the virus became more aggressive, cells that were resistant to infection emerged, displaying escape mechanisms operative at the level of viral entry, HCV RNA replication, or both. (nih.gov)

While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)
Most importantly, forced differentiation substantially reduces tumorigenicity of GBM cells in an in vivo xenotransplantation model. (lu.se)
Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)

The sialyltransferase ST6GAL1 that adds α2-6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. (nature.com)

Molvizarin has in vitro activity against tumor cells. (wikipedia.org)
Gambogic acid, the major active ingredient of gamboge, a brownish to orange resin exuded from the Garcinia hanburyi tree in Southeast Asia, possesses significant anticancer activity both in vitro and in vivo. (dovepress.com)

In the present study we show the reverse phenomenon in that TNF-alpha on the same progenitor cell population in combination with stem cell factor (SCF) and interleukin-7 (IL-7) through the p55 TNF receptor can recruit additional progenitors to proliferate. (ox.ac.uk)
This study aimed to clarify the direct role of IL-17 on tumor cells. (austinpublishinggroup.com)
In vitro study revealed the number of exposed cells decreased with lower proliferation rate than the non-exposed cells. (uai.ac.id)
In vitro experimental models to study the efficiency of the placental barrier for environmental toxicants: tumor cell lines versus trophoblast primary cells. (biomedicineandprevention.com)
This work will be of interest to researchers across tumor types and in other immunological fields of study. (elifesciences.org)
The aim of this study was to analyze the influence of low-level laser therapy (=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. (bvsalud.org)

Several trophoblast cell lines have been developed for in vitro studies of trophoblast physiology and in the following paragraphs we will give a brief overview on the cells have been used to develop in vitro models of the placental barrier. (biomedicineandprevention.com)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
In vivo tumorigenesis experiments, Tanezumab markedly alleviated nerve invasion of PC cells as well as relieved nociceptive conduction in animal models. (hindawi.com)
The scientists successfully monitored the behavior of individual immune cells and were thus able to describe their interactions with tumor models. (pasteur.fr)
Not surprisingly, the regulation of this vital safeguard has been studied in great detail for many years but mainly in tissue culture, or in vitro, models. (news-medical.net)
In vitro studies and animal models. (lu.se)

Anatomy21

Organisms11

Diseases79

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes49

Disciplines and Occupations1

Information Science4

Health Care6

Apoptosis18

Xenograft6

Reduced tumor growth1

Growth26

Metastasis11

Metastatic9

Inhibition2

Necrosis6

Mice8

Pathways4

Breast tumors1

Promote tumor cell1

Macrophages9

Macrophage1

Tissues6

Malignant6

Assays5

Antitumor3

Solid tumor1

Promotes tumor1

Inhibit1

Progression3

Cellular8

Antigen3

Cytotoxicity4

Pancreatic cancer cells1

Cultures3

Murine2

Subcutaneous1

Proliferation and viability2

Monoclonal2

Cytotoxic4

Biological3

Epithelial5

Cytokine3

Inhibitors1

Immunotherapy4

Lymphocytes1

Squamous cell carc4

Cancer21

Assay2

CSCs1

Prognosis1

Stem cell markers1

Proteins2

Findings3

Viability3

Adoptive transfer1

Chemical Compounds1

Mechanisms1

Differentiation3

Cancers1

Activity2

Study6

Models6