• One mole of stathmin binds to two moles of tubulin dimers through the stathmin-like domain (SLD). (wikipedia.org)
  • The single-ring structures, whose mean molecular weight depends on the specific peptide added to the reaction mixture, usually have sharp mass distributions corresponding, e.g., to rings containing eight tubulin dimers (when the added peptide is cryptophycin) and 14 dimers (e.g., with dolastatin). (nih.gov)
  • b) Size-exclusion chromatograms of binary complexes of tubulin:stathmin (blue) injected at 2:1.1 molar ratio, tubulin:TTL (orange) at 1:1.1 molar ratio and TTL alone (cyan). (nih.gov)
  • Structures of ternary complexes of tubulin with laulimalide/peloruside A and epothilone A are also solved, and a crosstalk between the laulimalide/peloruside and taxane sites via the M-loop of β-tubulin is found. (rcsb.org)
  • Stathmin, also known as metablastin and oncoprotein 18 is a protein that in humans is encoded by the STMN1 gene. (wikipedia.org)
  • Stathmin is a highly conserved 17 kDa protein that is crucial for the regulation of the cell cytoskeleton. (wikipedia.org)
  • Stathmin is a ubiquitous phosphorylated protein which makes it act as an intracellular relay for diverse regulatory pathways, functioning through a variety of second messengers. (wikipedia.org)
  • Regulation of stathmin is cell cycle dependent and controlled by the cell's protein kinases in response to specific cell signals. (wikipedia.org)
  • 19. Stathmin, a microtubule-destabilizing protein, is dysregulated in spinal muscular atrophy. (nih.gov)
  • At the molecular level, increased gene expression of SCG 10-like protein, a stathmin-related gene with microtubule destabilizing activity, was detected in the central nervous system of aging stathmin(-/-) mice. (wright.edu)
  • This gene encodes a protein which is a member of the stathmin protein family. (nih.gov)
  • Members of this protein family form a complex with tubulins at a ratio of 2 tubulins for each stathmin protein. (nih.gov)
  • Published by The Company of Biologists Ltd 2014 Microtubule Microtubule-associated protein MAP1S Microtubule dynamics Cytokinesis Regulating dynamic microtubule (MT) functions involves post-translational tubulin. (biologists.com)
  • Phosphorylation at four serine residues on stathmin named Ser16, Ser25, Ser38 and Ser63 causes weakened stathmin-tubulin binding. (wikipedia.org)
  • Stathmin phosphorylation increases the concentration of tubulin available in the cytoplasm for microtubule assembly. (wikipedia.org)
  • For cells to assemble the mitotic spindle necessary for initiation of the mitotic phase of the cell cycle, stathmin phosphorylation must occur. (wikipedia.org)
  • Stathmin (aka op18) can cause uncontrolled cell proliferation when mutated and not functioning properly. (wikipedia.org)
  • 20. Mechanism for the catastrophe-promoting activity of the microtubule destabilizer Op18/stathmin. (nih.gov)
  • Solution studies revealed that TTL binds at the polymerization, we investigated whether binding of edge of the tubulin dimer, making contacts mostly full-length stathmin and TTL to tubulin are mutually with -tubulin and possibly the protomer junction exclusive and whether TTL can form a triple complex where it can interfere with both longitudinal and with tubulin:stathmin and tyrosinate it efficiently. (nih.gov)
  • 6. Stathmin destabilizing microtubule dynamics promotes malignant potential in cancer cells by epithelial-mesenchymal transition. (nih.gov)
  • 11. RSK2 signals through stathmin to promote microtubule dynamics and tumor metastasis. (nih.gov)
  • At low concentrations of free tubulin, the growth rate at the microtubule ends is slowed and results in an increased rate of depolymerization (disassembly). (wikipedia.org)
  • The tendency of tubulin to switch stochastically between polymerization and depolymerization phases, termed dynamic instability ( Mitchison and Kirschner, 1984 ), facilitates remodeling of the microtubule cytoskeleton for its various roles. (rupress.org)
  • The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. (genetex.com)
  • These results suggest either that TTL and stathmin have a partially overlapping footprint on the tubulin dimer or that stathmin induces a tubulin conformation incompatible with stable TTL binding. (nih.gov)
  • the addition of the tubulin subunits lead to polymerisation and their loss, depolymerisation. (wikipedia.org)
  • Additionally, Stathmin is thought to have a role in cell signaling pathway. (wikipedia.org)
  • 5. KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway. (nih.gov)
  • 17. The stathmin phosphoprotein family: intracellular localization and effects on the microtubule network. (nih.gov)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tubulin Beta 1 (TUBb1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (scalegen.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tubulin Beta 1 (TUBb1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
  • Description: Quantitativesandwich ELISA kit for measuring Human Tubulin beta-1 chain (TUBB1) in samples from serum, plasma, tissue homogenates, cell lysates. (scalegen.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tubulin Beta 1 (TUBb1) in samples from tissue homogenates, cell lysates or other biological fluids. (scalegen.com)
  • Here we explore the mutual relationship between these proteins in vitro and demonstrate that full-length stathmin and TTL compete for binding to tubulin and fail to make a stable tubulin:stathmin:TTL triple complex in solution. (nih.gov)
  • Moreover, stathmin depresses TTL tubulin tyrosination activity in vitro. (nih.gov)
  • 9. Regulation of microtubule dynamic instability in vitro by differentially phosphorylated stathmin. (nih.gov)
  • Structural basis for the regulation of tubulin by vinblastine. (nih.gov)
  • The rate of microtubule assembly is an important aspect of cell growth therefore associating regulation of stathmin with cell cycle progress. (wikipedia.org)
  • Stathmin interacts with two molecules of dimeric α,β-tubulin to form a tight ternary complex called the T2S complex. (wikipedia.org)
  • 15. FOXM1 mediates resistance to docetaxel in gastric cancer via up-regulating Stathmin. (nih.gov)
  • Stathmin, and the related proteins SCG10 and XB3, contain a N-terminal domain (XB3 contains an additional N-terminal hydrophobic region), a 78 amino acid coiled-coil region, and a short C-terminal domain. (wikipedia.org)
  • The densities attributable to the microtubule-associated proteins lie in close proximity to helices 11 and 12 and the COOH terminus of tubulin. (rupress.org)
  • Here, using X-ray crystallography it is shown that laulimalide and peloruside A bind to a unique non-taxane site on β-tubulin and use their respective macrolide core structures to interact with a second tubulin dimer across protofilaments. (rcsb.org)
  • Mice without stathmin have deficiency in innate and learned fear. (wikipedia.org)
  • Stathmin-Deficient Mice Develop an Age-Dependent Axonopathy of the Cen" by Wolfgang Liedtke, Elizabeth E. Leman et al. (wright.edu)
  • Despite its abundant expression in the developing mammalian nervous system and despite its high degree of evolutionary conservation, stathmin-deficient mice do not exhibit a developmental phenotype. (wright.edu)
  • 1) Here we report that aging stathmin(-/-) mice develop an axonopathy of the central and peripheral nervous systems. (wright.edu)
  • At the functional level, electrophysiology recordings demonstrated a significant reduction of motor nerve conduction velocity in stathmin(-/-) mice. (wright.edu)
  • tubulin and stathmin were pre- incubated for 2 h followed by incubation with TTL for 40 min, resulting in final concentrations of 44, 24 and 44 M for tubulin, stathmin and TTL, respectively (black). (nih.gov)
  • The data further provide a structural framework for understanding the synergy observed between two classes of MSAs in tubulin assembly and the inhibition of cancer cell growth. (rcsb.org)
  • Stathmin−/− females do not assess threats well, leading to lack of innate parental care and adult social interactions. (wikipedia.org)
  • If stathmin is unable to bind to tubulin, it allows for constant microtubule assembly and therefore constant mitotic spindle assembly. (wikipedia.org)
  • 12. Characterization and detection of cellular and proteomic alterations in stable stathmin-overexpressing, taxol-resistant BT549 breast cancer cells using offgel IEF/PAGE difference gel electrophoresis. (nih.gov)
  • Stathmin also promotes microtubule disassembly by acting directly on the microtubule ends. (wikipedia.org)
  • Description: A sandwich ELISA kit for detection of Tubulin Beta 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (scalegen.com)
  • tubulin beta 2A class IIa [S. (gsea-msigdb.org)
  • tubulin beta 2B class IIb [S. (gsea-msigdb.org)
  • Tubulin partition between soluble and polymeric forms is tightly regulated in cells. (nih.gov)
  • 1. Stathmin expression alters the antiproliferative effect of eribulin in leiomyosarcoma cells. (nih.gov)
  • 8. Integrating mechanisms of response and resistance against the tubulin binding agent Eribulin in preclinical models of osteosarcoma. (nih.gov)
  • More specifically, stathmin is crucial in regulating the cell cycle. (wikipedia.org)
  • At cytokinesis, the last phase of the cell cycle, rapid dephosphorylation of stathmin occurs to block the cell from entering back into the cell cycle until it is ready. (wikipedia.org)
  • Their assembly is in part determined by the concentration of free tubulin in the cytoplasm. (wikipedia.org)
  • Together, these findings suggest that stathmin plays an essential role in the maintenance of axonal integrity. (wright.edu)
  • Our data further suggest that the evolutionarily maintained differences observed in the repeat domain may be important for the specific targeting of different repeats to either α or β tubulin. (rupress.org)
  • An unusual class of nanoscopic, ring-shaped, single-walled biopolymers arises when alphabeta-tubulin is mixed with certain small peptides obtained from various marine organisms and cyanobacteria. (nih.gov)
  • TTL is the disordered tubulin tails) of each of the - and - essential and also ubiquitously expressed. (nih.gov)