Loading...
  • subunits
  • Once this structure is formed, katanin hydrolyzes ATP, and likely undergoes a conformational change that puts mechanical strain on the tubulin subunits, which destabilizes their interactions within the microtubule lattice. (wikipedia.org)
  • residues
  • Synthetic peptide (Human) conjugated to KLH, corresponding to C terminal residues of Human alpha tubulin. (abcam.com)
  • Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. (wikipedia.org)
  • ATAT1 contains a conserved surface pocket close to the active site composed largely of hydrophobic and basic residues, which likely complement the acidic loop containing α-tubulin K40. (wikipedia.org)
  • See tubulin polyglutamylase) polyglycylation, covalent linkage of one to more than 40 glycine residues to the tubulin C-terminal tail butyrylation gamma-carboxylation dependent on Vitamin K glycosylation, the addition of a glycosyl group to either arginine, asparagine, cysteine, hydroxylysine, serine, threonine, tyrosine, or tryptophan resulting in a glycoprotein. (wikipedia.org)
  • dimers
  • 2 ) harness the power of recent developments in recombinant tubulin engineering ( 3 ⇓ ⇓ - 6 ) and interferometric scattering microscopy (iSCAT) ( 7 ) to measure directly the association and dissociation constants of single tubulin dimers at the growing microtubule tip ( k On and k Off , respectively) and advance a model for microtubule growth. (pnas.org)
  • cytoskeleton
  • accumulation of tubulin and microtubular cytoskeleton are induced during seed imbibition and that cell division may occur as a post-germination phenomenon with radicle protrusion occurring by cell elongation, or as a pre-germination phenomena (DE CASTRO et al. (thefreedictionary.com)
  • Incorporation of these kinases into the cytoskeleton via the GBP-1/ class III β-tubulin interaction protects kinases from rapid degradation. (wikipedia.org)
  • polymerization
  • While the cytotoxins used in the majority of advanced programs in the field prevent tubulin polymerization during cell division, Synthon's differentiating linker-drug technology - applying valine-citrulline-seco-DUocarmycin- hydroxyBenzamide-Azaindole (vc-seco-DUBA) - is based on synthetic duocarmycin analogs, which bind to the minor groove of DNA and subsequently cause irreversible alkylation of DNA. (thefreedictionary.com)
  • KX2-391, also known as KX-01, is a novel dual Src kinase and tubulin polymerization inhibitor that Athenex is developing into a topical formulation for the treatment of actinic keratosis. (thefreedictionary.com)
  • By visualizing the polymerization of injected fluorescent tubulin, we show that substantial microtubule polymerization occurs in neurons grown at reported vinblastine concentrations. (rupress.org)
  • Sensitization of taxol-induced apoptosis by curcumin involves down-regulation of nuclear factor-kappa B and the serine/threonine kinase Akt and is independent of tubulin polymerization. (thefreedictionary.com)
  • Of particular interest are the effects of post-translational modifications on binding, bending, and polymerization, and as well as the corresponding changes in tubulin structure and dynamics as probed by NMR. (weizmann.ac.il)
  • molecular
  • Other pro-survival factors interacting with class III β-tubulin enabling cellular adaptation to oxidative stress include the molecular chaperone HSP70/GRP75. (wikipedia.org)
  • neurons
  • Tubulin transport in neurons. (rupress.org)
  • It is possible to use monoclonal antibodies and immunohistochemistry to identify neurons in samples of brain tissue, separating neurons from glial cells, which do not express Class III β-tubulin. (wikipedia.org)
  • TUBB2C
  • In structural terms, constitutive Class I (TUBB) and Class IVb (TUBB2C) β-tubulins contain a cysteine at position 239, while βIII-tubulin has a cysteine at position 124. (wikipedia.org)
  • actin
  • Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin with ~42 kDa. (wikipedia.org)
  • molecule
  • One molecule of GTP is tightly bound to the α-tubulin and is non-exchangeable whereas the other GTP molecule is bound to β-tubulin and can be easily exchanged with guanosine diphosphate (GDP). (wikipedia.org)
  • cell biology
  • Experience and expertise in NMR, protozoa cell biology or tubulin biochemistry, or relevant expertise that will bring a unique contribution to the project. (weizmann.ac.il)
  • axon
  • It is of particular interest because of the history of conflicting results concerning the form in which tubulin is transported in the axon. (rupress.org)
  • mitotic
  • Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. (abcam.com)
  • tubulin is differentially expressed in mitotic and non-mitotic cardiomyocytes in the regenerating zebrafish heart. (abcam.com)
  • HeLa
  • Western blot analysis of extracts from HeLa, NIH/3T3, KNRK cells and rat brain using β-Tubulin (D3U1W) Mouse mAb. (cellsignal.com)
  • paclitaxel
  • These variations in primary structure affect the paclitaxel (a mimic of Nur77) binding domain on βIII-tubulin and may account for the ability of this isotype to confer resistance to Nur77-initiated apoptosis. (wikipedia.org)
  • First drug belong to the taxanes and paclitaxel, discovered in extracts from the bark of the yew tree, Taxus brevifolia, in 1967 by Monroe Wall and Mansukh Wani but, its tubulin inhibition activity was not known until 1979. (wikipedia.org)
  • neural development
  • Congenital neurologic syndromes associated with TUBB3 missense mutations demonstrate the critical importance of class III β-tubulin for normal neural development. (wikipedia.org)
  • Human
  • Human α-tubulin subtypes include:[citation needed] TUBA1A TUBA1B TUBA1C TUBA3C TUBA3D TUBA3E TUBA4A TUBA8 All drugs that are known to bind to human tubulin bind to β-tubulin. (wikipedia.org)
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Tubulin (D3U1W) Mouse mAb. (cellsignal.com)
  • Immunohistochemical analysis of paraffin-embedded human large cell carcinoma of the lung using β-Tubulin (D3U1W) Mouse mAb. (cellsignal.com)
  • Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using β-Tubulin (D3U1W) Mouse mAb. (cellsignal.com)
  • contrast
  • By contrast, cytoplasmic HuR and miR-200c enhance class III β-tubulin translation by facilitating the entry of the mRNA into the ribosome. (wikipedia.org)