In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)
Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process. (biomedcentral.com)
Although not essential for initial WD formation, the later differentiation of this embryological structure relies on androgens derived from the Leydig cells. (esur2023.com)
In the presence of a Y chromosome and the SRY gene, müllerian‐inhibiting substance produced by Sertoli cells causes regression of the MD. Meanwhile, testosterone produced by the Leydig cells induces differentiation of the WD into the efferent ducts that communicate with the rete testis, followed by the epididymis, ductus deferens, seminal vesicle, and ejaculatory duct. (esur2023.com)
It penetrates mesenchyme on the nephrogenic ridge, which is known as the metanephric blastema, and induces differentiation into renal parenchyma. (medscape.com)
It is acknowledged that nephron develops after bilateral induction of the metanephric mesenchyma and branching ureteric bud (UB), and that nephrogenic rest and Wilms' tumor (nephroblastoma) arises from impaired differentiation of metanephric blastema. (bvsalud.org)
Ureteric bud and metanephric1
The doctor explains to the mother that this is most likely due to aberrant interaction between the ureteric bud and metanephric mesenchyme. (osmosis.org)
Renal4
By about 20 weeks, the ureteric bud has formed the ureters , the renal calyces, collecting ducts, and collecting tubules, while the metanephric blastema develops into the nephron itself, which includes the epithelial cells and the podocytes of the Bowman's capsule. (osmosis.org)
With renal agenesis , the ureteric bud fails to induce development of the metanephric blastema, and so either one or both kidneys don't develop. (osmosis.org)
The ureteric bud grows laterally and invades the center of the metanephric blastema, the primordial renal tissue [4]. (esur2023.com)
The ureteric bud divides and branches forming the collecting system (renal pelvis, infundibulae, calyces, and tubules) for urine drainage. (esur2023.com)
Mesenchyme2
The cells of the pronephric duct migrate caudally whilst inducing adjacent mesenchyme to form the tubules of the initial kidney-like structure called the pronephros. (wikipedia.org)
The permanent kidney of amniotes, the metanephros, develops during the 10th week in human embryos and is formed by the reciprocal interactions of the metanephrogenic blastema (or metaneophrogenic mesenchyme) and the ureteric bud. (wikipedia.org)
Nephrogenic2
Moreover, CA2-positive tubular structures and ATP6V1B1- and ATP6V0D2-positive immature non-α- and non-ß-intercalated cells were detected in nephrogenic rest and Wilms' tumor. (bvsalud.org)
We suggest that Wilms' tumor is more than nephroblastoma and propose a definition that Wilms tumor is a malignant embryonal neoplasm derived from pluripotential cells of nephrogenic blastema and of ureteric bud tip. (bvsalud.org)
Mesonephros4
In mammals however, the pronephric tubules and the anterior portion of the pronephric duct degenerates in 3.5 weeks to be succeeded by the mesonephros, the embryonic kidney. (wikipedia.org)
The mesonephros is constituted of a set of new tubules formed from the lateral and ventral sides of the gonadal ridge joining the cloaca. (wikipedia.org)
Despite its transiency, the mesonephros is crucial for the development of structures such as the Wolffian duct (or mesonephric duct), which in turn gives rise to the ureteric bud of the metanephric kidney. (wikipedia.org)
The mesonephros is a functional primitive kidney with excretory tubules which drain into the WD for a brief period [3], Fig. 1. (esur2023.com)
Kidney4
Alright so during fetal development, first off, you've got this structure called the mesonephric duct which is involved in development of urinary and reproductive organs , and during the 5th week of gestation, a little guy called the ureteric bud starts pushing its way into another structure called the metanephric blastema, and together, these two little embryologic structures go on to develop into a kidney. (osmosis.org)
The adult kidney, also referred to as the metanephric kidney, forms at the posterior end of the intermediate mesoderm after the degeneration of previous, less complex kidney structures. (wikipedia.org)
ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
The kidney is a complex organ composed of several cell types, and it is conformed approximately by 11,000 nephrons. (biomedcentral.com)
Forms1
The metanephric blastema forms glomeruli, proximal and distal tubules. (esur2023.com)
Adult1
Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
System1
[ 8 ] The ureteral bud undergoes approximately 15 generations of division to complete the collecting system from collecting tubules proximally to the hemitrigone of the bladder distally. (medscape.com)
Types1
Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
Mouse1
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Positive2
Gonadal derived neurotrophic factor (GDNF) secreted by the metanephrogenic blastema activates the receptor tyrosine kinase RET, via the co-receptor GFRα1 and triggers outgrowth of Ret positive cells from the nephric duct towards the GDNF signal, promoting ureteric bud outgrowth and invasion. (wikipedia.org)
Tubules surrounded by tumorous blastemal cells resembling UB tip were positive for RET, ROBO1, and SLIT2 in Wilms' tumor. (bvsalud.org)
Metanephrogenic blastema3
The permanent kidney of amniotes, the metanephros, develops during the 10th week in human embryos and is formed by the reciprocal interactions of the metanephrogenic blastema (or metaneophrogenic mesenchyme) and the ureteric bud. (wikipedia.org)
Gonadal derived neurotrophic factor (GDNF) secreted by the metanephrogenic blastema activates the receptor tyrosine kinase RET, via the co-receptor GFRα1 and triggers outgrowth of Ret positive cells from the nephric duct towards the GDNF signal, promoting ureteric bud outgrowth and invasion. (wikipedia.org)
Bone morphogenetic protein ( BMP ) signaling in the surrounding mesoderm is also inhibitory to outgrowth of the ureteric bud, but within the metanephrogenic blastema its action is counteracted by the BMP-inhibitory actions of gremlin , which is produced within the blastema itself. (clinicalgate.com)
Nephric2
Lim-1 is required for the aggregation of the mesenchymal cells of the intermediate mesoderm into the primary nephric ducts. (clinicalgate.com)
During its caudal migration, the nephric duct induces 3 embryonic kidneys in the nephrogenic cord: pronephros, mesonephros, and metanephros (in a spacial and temporal sequence). (medscape.com)
Epithelial1
However, most studies of tubular ion transport have been performed in cell monolayers grown on or epithelial sheets affixed to a flat support, since analysis of transepithelial transport in native tubules by in vitro microperfusion requires considerable expertise. (bvsalud.org)
Vitro1
Here, we report on the generation and characterization of an in vitro, perfusable three-dimensional kidney CCD model (3D CCD), in which immortalized mouse PC-like mpkCCD cells are seeded within a cylindrical channel embedded within an engineered extracellular matrix and subjected to luminal fluid flow. (bvsalud.org)
Mesoderm3
A link has been established between Vg1/Nodal signalling and BMP signalling, whereby Vg1/Nodal signalling regulates intermediate mesoderm formation by modulating the growth-inducing effects of BMP signalling. (wikipedia.org)
The adult kidney, also referred to as the metanephric kidney, forms at the posterior end of the intermediate mesoderm after the degeneration of previous, less complex kidney structures. (wikipedia.org)
1 recently reported in Nature Biotechnology a protocol to generate functional collecting duct cells from human pluripotent stem cells. (bvsalud.org)
METHODS: Selective ablation of Dicer in AQP2-expressing cells (DicerAQP2Cre+ mice) was used to investigate the role of miRNAs in the kidney collecting duct of mice. (bvsalud.org)
Kidneys2
These early forms of the kidney are later supplanted by the definitive metanephric kidneys, but as they regress, certain components are retained to be reused by other components of the urogenital system. (clinicalgate.com)
During the early phases of migration of the metanephric kidneys, the mesonephric kidneys regress. (clinicalgate.com)
Expression3
BACKGROUND: MicroRNAs (miRNAs), formed by cleavage of pre-microRNA by the endoribonuclease Dicer, are critical modulators of cell function by post-transcriptionally regulating gene expression. (bvsalud.org)
We demonstrated that MCs can be loaded into shed microvesicles with greater efficiency than their parental plasmid counterparts and that microvesicle-mediated MC delivery led to significantly higher and more prolonged transgene expression in recipient cells than microvesicles loaded with the parental plasmid. (regenerativemedicine.net)
Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)