Tsp 1 expression with extracellular. Medical search. Frequent questions

CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). (wikipedia.org)
CD47 is a high affinity receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis, and in this later capacity the TSP1-CD47 interaction inhibits nitric oxide signaling at multiple levels in vascular cells. (wikipedia.org)
To address the conflicting role of thrombospondin (TSP)-1 reported in acute and chronic pathologies, this study investigated the role of TSP-1 in regulating leukocyte recruitment and regulation of VCAM-1 expression using mouse models of uveitis. (bvsalud.org)
We tested whether reduced tear levels of immunoregulatory thrombospondin (TSP)-1, which also inhibits matrix metalloproteinase (MMP)-9, would reflect SS pathogenesis aiding the identification of patients with SS-dry eye. (bvsalud.org)
CTRP is a type 1 transmembrane protein, containing two adhesive domains in its extracellular portion, an A-domain of von Willebrand factor and a thrombospondin type I repeat (TSR, TSP). (pberghei.eu)
COMP is a 524-kDa homopentameric extracellular matrix glycoprotein and a member of the thrombospondin (TSP) family [ 8 ]. (biomedcentral.com)
In a retrospective study of 171 patients with NSCLC, OPN and thrombospondin-1 (a matrix protein that induces proliferating endothelial cells to secrete Fas ligand, which mediates apoptosis) levels were compared. (shu.edu)
Abstract Background Thrombospondin-2 (TSP-2) is a secreted matricellular glycoprotein that is found to mediate cell-to-extracellular matrix attachment and participates in many physiological and pathological processes. (tmu.edu.tw)
Endogenous inhibitors of angiogenesis include thrombospondin 1 (TSP-1) and a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1), and their aberrant expression may contribute to the diabetes-related dysregulation of retinal vascular homeostasis and vasculopathies ( 7 , 8 ). (spandidos-publications.com)
The Role of Thrombospondin-1 (TSP-1) in Epithelial Ovarian Cancer. (mcmaster.ca)
In a previous study using the freeze-lesion (FL) murine model of DCM, we found that levels of thrombospondin (TSP) and the calcium channel auxiliary subunit α2δ‐1 were elevated. (eneuro.org)

The botanical glycoside, oleandrin, derived from extracts of Nerium oleander , has previously been shown to reduce the levels of the gp120 envelope glycoprotein on human immunodeficiency virus type-1 (HIV-1) particles and inhibit HIV-1 infectivity in vitro . (longdom.org)
The botanical glycoside, oleandrin, and an extract of Nerium oleander have been shown to prevent the incorporation of the gp120 envelope glycoprotein of HIV-1 into mature virus particles and inhibit viral infectivity in vitro [ 1 ]. (longdom.org)

Binding of TSP-1 to CD47 influences several fundamental cellular functions including cell migration and adhesion, cell proliferation or apoptosis, and plays a role in the regulation of angiogenesis and inflammation. (wikipedia.org)
The apoptosis inducing function of CD47 appears to be dependent on activation of specific epitopes on the extracellular domain. (wikipedia.org)
Treatment with a disulfide-linked antibody dimer induces apoptosis of CD47-positive primary B-CLL and leukemic cells (MOLT-4 and JOK-1). (wikipedia.org)
Apoptosis induction appears to be regulated by the hypoxia inducible factor 1α (HIF-1α) pathway. (wikipedia.org)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

These effects by MSCs are mediated by a variety of factors, including growth factors, cytokines and extracellular vesicles (EVs). (techscience.com)
Exosomes are a kind of extracellular vesicles (EVs), which was first reported by Pan and Johnstone in 1983 as unwanted cellular components extruding from reticulocytes. (bbrc.in)

Consistent with M1 polarization, the MWCNTs induced the production and secretion of proinflammatory cytokines tumor necrosis factor- and interleukin-1, and proinflammatory LMs leukotriene B4 (LTB4) and prostaglandin E2 (PGE2). (cdc.gov)
10, 11] However, serum IgE levels do not positively or negatively correlate with production or expression of these cytokines. (medscape.com)

Besides being the first heterologous expression and characterization of a gene coding for a lipase from A. alcalophilum , this report shows that LipA is very versatile exhibiting both acetylxylan esterase and lipase activities potentially useful for diverse industry sectors, and that tobacco is a suitable bioreactor for producing fungal proteins. (biomedcentral.com)
As envisioned, since they had been isolated from extracellular, pre-house rudiments, oikosins had been generally classified as secreted proteins. (calpaininhibitor.com)
In situ stains for the functions as an extracellular factor stabilizing the extracellular matrix (ECM) by cross-linking ingredient proteins such as collagen and laminin [19]. (calpaininhibitor.com)
The spiral of Ca2+ in H+ requirements is prescribed to routes which are phosphorylated proteins and frequently listen Ca2+ audiences in absent XH2A( as 1 TGN). (erik-mill.de)
Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. (rndsystems.com)
The expression levels of various mRNAs and proteins were measured by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting, respectively. (spandidos-publications.com)
Levels of proteins, including Cyt C, Bcl-2/BAX, and Nrf2 /HO-1-associated proteins, were determined by western blotting and immunofluorescence. (sdbonline.org)

Expression in equine cutaneous tumors has been reported as well. (wikipedia.org)
The expression profile of TSP-2 on tumors is controversial, and it up-regulates in some cancers, whereas it down-regulates in others, suggesting that the functional role of TSP-2 on tumors is still uncertain. (tmu.edu.tw)

TSP binds to α2δ‐1 to drive the formation of excitatory synapses during development, suggesting that overactivation of this pathway may lead to exuberant excitatory synaptogenesis and network hyperexcitability seen in DCMs. (eneuro.org)

Recent progresses in related experiments concerning cell interactions with extracellular matrix have demonstrated that substrate rigidities can exert a remarkable influence on cell adhesion and spreading dynamics. (techscience.com)
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. (cusabio.com)
TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. (cusabio.com)
this file supplies the natural data of preliminary experiments performed using 2 enzyme dilutions in order to discover out the dilution that greatest fitted with requirements 1 and 2 described in the outcomes portion of the manuscript (automated sugars releasing assay: substrate focus). (tam-receptor.com)
Experimental Approach: Here we show an IBD therapeutic candidate LMT503, a substrate which modulates NADH quinone oxidoreductase 1, which enhances NAD+ and induce anti-inflammatory macrophage polarization. (21docs.com)

OPN is encoded by the SPP1 gene , which is comprised of 7 exons and is located on the long arm of chromosome 4 near four other SIBLING (small integrin-binding N-linked glycoproteins) that are contained in bine and dentin - bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE). (shu.edu)
Results Based on the gene expression omnibus database and immunohistochemistry, we found that TSP-2 increased with the progression of PCa, especially in metastatic PCa and is correlated with the matrix metalloproteinase-2 (MMP-2) expression. (tmu.edu.tw)
Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules. (rndsystems.com)

Fig. 1: Radiation exposure in healthy lung tissue enhances metastasis. (nature.com)
Conclusions Taken together, our results indicate that TSP-2 enhances the migration of PCa cells by increasing MMP-2 expression through down-regulation of miR-376c expression. (tmu.edu.tw)

The molecular mechanism of TSP-2 on prostate cancer (PCa) metastasis was investigated through pharmaceutical inhibitors, siRNAs, and miRNAs analyses. (tmu.edu.tw)
The role of TSP-2 on PCa metastasis in vivo was verified through xenograft in vivo imaging system. (tmu.edu.tw)
On the other hand, the animal studies revealed that the bone metastasis was abolished when TSP-2 was stably knocked down in PCa cells. (tmu.edu.tw)

CD47 is a 50 kDa membrane receptor that has extracellular N-terminal IgV domain, five transmembrane domains, and a short C-terminal intracellular tail. (wikipedia.org)

Thus, our study identifies TSP-1:CD47 interaction as a molecular pathway that modulates IL-17-mediated VCAM-1 expression, contributing to its anti-inflammatory effect in chronic inflammatory conditions. (bvsalud.org)
Three molecular mechanisms involved in tumor dormancy have been identified: cellular dormancy (quiescence or mitotic arrest), angiogenic dormancy (limited tumor size), and immunologic dormancy (immunosurveillance, balance between clearance and proliferation) (Figure 1 ). (biomedcentral.com)
Here, we used mice with a genetic deletion of α2δ‐1 to determine how α2δ‐1 contributes to cell death, elevated excitatory synapse number, and in vitro network function after FL and to examine the molecular specificity of GBP's effects. (eneuro.org)
CONCLUSION: Among all the reported methods for detection of HTLV-1, only serological and molecular tests are used as the most common technical assays for HTLV-1. (afpm.org.my)

Independent of HIFα, hypoxia induces OPN expression in breast cancer cells, which leads to NFkB activation, VEGF expression , and angiogenesis. (shu.edu)
Treatment with 30 mM glucose also increased the expression of vascular endothelial growth factor (VEGF) mRNA and protein, and the expression levels of VEGF mRNA and protein were suppressed by kaempferol (10 and 30 µM). (spandidos-publications.com)
A study has demonstrated that activation of the peroxisome proliferator-activated receptor g coactivator 1-α (PGC-1α)/ERRα pathway increases VEGF expression and angiogenesis in endothelial cells, which suggests that ERRα may be involved in the pathogenesis of DR ( 10 ). (spandidos-publications.com)

Activation of CD47 with TSP-1 in wild-type cells inhibits proliferation and reduces expression of stem cell transcription factors. (wikipedia.org)

Treatment of murine macrophages (J774A.1) with Mitsui-7 MWCNTsincreased the expression of Alox5 mRNA and protein in a concentration- and time-dependent manner. (cdc.gov)
RT‑qPCR and western blotting results showed that treatment with 30 mM glucose for 12, 24 and 48 h increased the expression level of estrogen‑related receptor α (ERRα) mRNA and protein. (spandidos-publications.com)

There are two ways that OPN can be exploited - (1) diagnostic/prognostic biomarker, and (2) therapeutic target. (shu.edu)
Taken together these studies suggests that inhibiting α2δ-1 signaling may have therapeutic promise to reduce cell death and network reorganization associated with insult-induced DCMs. (eneuro.org)

These findings prompted us to investigate whether oleandrin could similarly inhibit another related retrovirus, the HTLV-1. (longdom.org)

In a chronic uveitis model, induced by immunizing wild-type mice with specific interphotoreceptor retinoid-binding protein (IRBP) peptide, topically applied TSP-1-derived CD47-binding peptide significantly reduced the clinical disease course and retinal leukocyte adhesion as compared to the control peptide-treated group. (bvsalud.org)
The Sm -TSP-2 schistosomiasis vaccine is comprised of a 9 kDa recombinant protein corresponding to the extracellular domain of a unique S. mansoni tetraspanin. (bcm.edu)
Sm -TSP-2 was expressed as a recombinant protein secreted by the yeast Pichia Pink , and purified in a two-step process, which resulted in a protein recovery yield of 31 percent and a protein purity of 97 percent The developed processes are suitable for the production of purified protein for subsequent formulation and Phase 1 clinical studies. (bcm.edu)
Simvastatin dose dependently inhibited THP-1 cell migration mediated by monocyte chemoattractant protein 1, with a 50% inhibitory concentration of about 50 nM. (neuroprobe.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

The spontaneously increased VCAM-1 expression and leukocyte adhesion in retinas of TSP-1-deficient mice suggested a TSP-1-mediated regulation of VCAM-1 expression. (bvsalud.org)
In contrast, in LPS-mediated acute uveitis, TSP-1 deficiency significantly reduced the retinal leukocyte adhesion. (bvsalud.org)
Such reduced endogenous TSP-1 expression in IL-17-stimulated ECs helps limit the CD36-mediated increased VCAM-1 expression, while favoring CD47-mediated inhibition of VCAM-1 expression and leukocyte adhesion. (bvsalud.org)

Expression and localization of vascular endothelial growth factor and angiopoietin-1 and their receptors FLT-1, FLK-1, and TIE-2 in bovine ovaries during follicular and luteal development. (mcmaster.ca)

Methods The expression of TSP-2 on prostate cancer progression was determined in the tissue array by the immunohistochemistry. (tmu.edu.tw)

The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. (cusabio.com)

Activation of CD47 with TSP-1 increases proliferation of human U-87 MG and U373 astrocytoma cells but not normal astrocytes. (wikipedia.org)
We also find that GBP can reduce FL-induced increases in excitatory synapse number in α2δ-1 knock-out (KO) mice, indicating GBP has α2δ-1-independent anti-synaptogenic effects. (eneuro.org)

We therefore tested whether oleandrin or an extract from N. oleander could also inhibit the infectivity of the human T-cell leukemia virus type-1 (HTLV-1) -a related enveloped retrovirus and emerging tropical infectious agent. (longdom.org)
BACKGROUND: Human T-lymphotropic virus Type 1 (HTLV-1) is a retrovirus that is endemic in some regions of the world. (afpm.org.my)
Human Immunodeficiency Virus (HIV) Infection in Infants and Children Human immunodeficiency virus (HIV) infection is caused by the retrovirus HIV-1 (and less commonly by the related retrovirus HIV-2). (msdmanuals.com)

Katagiri K, Itami S, Hatano Y, Yamaguchi T, Takayasu S. In vivo expression of IL-4, IL-5, IL-13 and IFN-gamma mRNAs in peripheral blood mononuclear cells and effect of cyclosporin A in a patient with Kimura's disease. (medscape.com)

OPN induces the expression of oxidoreductases, which protects cells from anoikis during anchorage independent growth. (shu.edu)
OPN induces gene expression changes in a model of breast cancer that reflect the six hallmarks of cancer. (shu.edu)

With inhibition of p38, ERK, and JNK, the TSP-2-induced cell migration and MMP-2 expression were abolished, indicating that the TSP-2's effect on PCa is MAPK dependent. (tmu.edu.tw)

It is well documented that increased expression of LH receptor (LHR) in granulosa cells (GC) and specific changes of intrafollicular factors such as the insulin-like growth factor (IGF) and inhibin-activin-follistatin systems play a critical role in E 2 production in the DF [ 6 , 7 ]. (biomedcentral.com)
Cetirizine forms a complex with histamine for H 1 -receptor sites in blood vessels, the gastrointestinal (GI) tract, and the respiratory tract. (medscape.com)
This study demonstrated that the Receptor for Activated C-Kinase 1 (RACK1) is important in SC function. (sdbonline.org)

The expression and activity of inducible nitric oxide synthase, an intracellular marker of M1, were increased by MWCNTs. (cdc.gov)

To evaluate whether statins influence the proinflammatory responses of monocytic cells, we studied their effects on the chemotactic migration and MMP-9 secretion of human monocytic cell line THP-1. (neuroprobe.com)
A few unique oikosin families were being detected in the large Eisen area (Fig. three), 1 of which has eight paralogs, oik24a-h (Fig. S4), which with each other with the chain of pearls represent the place of if secretion. (calpaininhibitor.com)

In that study, antagonizing TSP/α2δ‐1 signaling using the drug gabapentin (GBP) reduced many FL-induced pathologies. (eneuro.org)
We identified a critical role for α2δ-1 in FL-induced pathologies and in mediating the neuroprotective effects of GBP. (eneuro.org)

In addition, administration of the antibody prolonged survival of SCID mice implanted with JOK-1 cells. (wikipedia.org)
Biotin-labeled TGF-β1 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody. (cusabio.com)
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution. (cusabio.com)

Furthermore, microarray profiling identified 226 genes with differential expression in the liver of animals treated with MSC, with cellular signaling, and actin cytoskeleton regulation as key upregulated processes. (frontiersin.org)

Global gene expression profiles of F1 (10.7 +/- 0.7 mm) and F2 (7.8 +/- 0.2 mm) were analyzed by hierarchical cluster analysis and expression profiles of 16 representative genes were confirmed by QPCR analysis. (biomedcentral.com)
Some studies compared the gene expression profiles between DF and SF around the time of follicular selection. (biomedcentral.com)
Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)

Expression of several stem cell markers, including c-Myc, is elevated in CD47-null endothelial cells and a human T cell line lacking CD47. (wikipedia.org)

The results of our in vitro study, using vascular endothelial cell (EC) cultures, demonstrate that unlike TNF-α, VCAM-1 expression induced by IL-17 is associated with a reduced expression of endogenous TSP-1. (bvsalud.org)
These results demonstrated that both oleandrin and the crude phytoextract inhibited the formation of virological synapses and the transmission of HTLV-1 in vitro . (longdom.org)

The bulk RNA sequencing datasets ( GSE180823 ) and the single-cell RNA sequencing datasets ( GSE181306 ) are deposited in the Gene Expression Omnibus (GEO, NCBI) repository. (nature.com)

Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. (sdbonline.org)
This is the first direct demonstration of α2δ-1's role in injury-induced cell death in the CNS, and suggests that α2δ-1 may be broadly involved in pathologic changes after neonatal brain insult. (eneuro.org)

Due to the ubiquitous expression of CD47, signaling differs according to cell type. (wikipedia.org)
The RAS-transformed cell lines MDFB6 and B6ras show near complete loss of TSP-1 expression. (wikipedia.org)
Additionally, through binding to CD36 and integrin ανβ3, TSP-2 increased cell migration and MMP-2 expression. (tmu.edu.tw)
Furthermore, the TSP-2-induced MMP-2 expression and the subsequent cell motility were suppressed upon miR-376c mimic stimulation. (tmu.edu.tw)
Cell Death Discov 8(1): 459. (sdbonline.org)
Cell Div 17(1): 4. (sdbonline.org)
α2δ-1 signaling drives apoptotic cell death, anatomic reorganization, excitatory synaptogenesis, astrocytosis, and network hyperexcitability in a model of insult-induced cortical malformation known as freeze lesion (FL). (eneuro.org)
It is known to cause several diseases like adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). (afpm.org.my)
In addition to immune defects that affect IgE synthesis, defects of cell-mediated immunity have also been reported, consistent with impaired TH 1 responses. (medscape.com)
Human immunodeficiency virus (HIV) infection results from 1 of 2 similar retroviruses (HIV-1 and HIV-2) that destroy CD4+ lymphocytes and impair cell-mediated immunity, increasing risk of certain infections and cancers. (msdmanuals.com)

2016. 14(1):1-5. (medscape.com)

In this study, we tried to identify differentially expressed genes between largest (F1) and second-largest follicles (F2), and classify them by global gene expression profiling using a combination of microarray and quantitative real-time PCR (QPCR) analysis. (biomedcentral.com)
This extracellular, non-particular "sticky" area on pre-property rudiments was systematically excluded in the evaluation of all staining designs in this study. (calpaininhibitor.com)

CTRP has six tandemly arrayed A domains followed by seven TSP type 1-like domains. (pberghei.eu)
It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)

Tear TSP-1 and MMP-9 levels were measured using a custom magnetic bead-based multi-plex assay in a masked manner. (bvsalud.org)
however, TSP‑1 and ADAMTS‑1 levels did not differ between high glucose and normal (5 mM) glucose conditions. (spandidos-publications.com)

Their metalloproteinase domains have sequence similarity to extracellular Zn-dependent metalloproteinases of the astacin form. (calpaininhibitor.com)

Anatomy37

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