In addition to trypsin, plasmin, thrombin, tryptase and matrix metalloprotease-2 each yielded E-LDL particles with high C1-activating efficiency, and the C1 activation extent was strictly dependent on cholesterol esterase treatment in all cases. (europa.eu)
Proteases present in blood serum (thrombin, plasmin, Hageman factor, etc.) play an important role in blood-clotting, as well as blood clot lysis, and the correct action of the immune system. (stratech.co.uk)
An amount of PIXI which inhibited by 50% factor XIa cleavage of the chromogenic substrate S2366 (Pyr-Glu-Pro-Arg-pNA-2H2O) only slightly inhibited (5-9%) factor XIIa, plasma kallikrein, plasmin, and activated protein C and did not inhibit factor Xa, thrombin, tPA, or trypsin, suggesting specificity for factor XIa. (qxmd.com)
Proteases4
the Kunitz/bovine pancreatic trypsin inhibitor family, they inhibit proteases of the S1 family [ ( PUBMED:14705960 ) ] and are restricted to the metazoa with a single exception: Amsacta moorei entomopoxvirus. (embl.de)
Trypsin-like serine proteases are crucial for influenza activation by mediating the cleavage of the viral surface glycoprotein HA and hence promoting the fusion potential of the virus. (uni-marburg.de)
Acid proteases secreted into the stomach (such as pepsin) and serine proteases present in duodenum (trypsin and chymotrypsin) enable us to digest the protein in food. (stratech.co.uk)
The assay utilizes a fluorescent casein conjugate that is proven to be a generic substrate for a broad spectrum of proteases (e.g. trypsin, chymotrypsin, thermolysin, proteinase K, protease XIV, and elastase). (stratech.co.uk)
Inhibitors5
The pancreatic trypsin inhibitor (Kunitz) family [ ( PUBMED:6996568 ) ( PUBMED:1703675 ) ( PUBMED:1593645 ) ] is one of the numerous families of serine proteinase inhibitors. (embl.de)
Plasma inter-alpha-trypsin inhibitor (I alpha TI) is a major representative of the superfamily of Kunitz-type protease inhibitors in mammals. (embl.de)
However, the tested monobasic compounds were still decent matriptase inhibitors that are additionally suitable for cell culture and animal studies in their benzamidine prodrug forms, which are well established from related inhibitors of thrombin. (uni-marburg.de)
Other anticoagulant substance classes like vitamin K antagonists or thrombin inhibitors are considered to have a higher bleeding risk than factor Xa inhibitors. (inteligand.com)
Some of them can detach the terminal amino acids from the protein chain (exopeptidases, such as aminopeptidases, carboxypeptidase A), while others attack internal peptide bonds of a protein (endopeptidases, such as trypsin, chymotrypsin, pepsin, papain and elastase). (stratech.co.uk)
High activities of trypsin, chymotrypsin LAP , and α-amylase, as well as overexpression of il-10 in the spleen, were detected in fish feed 0.5% D. hansenii . (pensoft.net)
Receptors1
Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. (wikipedia.org)
Thrombin activates the receptor on the surface of platelets inducing platelet aggregation, granular secretion, and procoagulant activity. (enzolifesciences.com)
Receptor-activati2
Cell lines were evaluated for their sensitivity (calcium signalling) towards trypsin and the receptor-activating peptides, SLIGRL-NH2, SLIGEL-NH2, trans-cinnamoyl(tc)-LIGRLO-NH2, and SFLLR-NH2. (nih.gov)
An enzyme substrate which permits the measurement of peptide hydrolase activity, e.g. trypsin and thrombin. (nih.gov)
Inhibit1
Endogenous factors and drugs that directly inhibit the action of THROMBIN, usually by blocking its enzymatic activity. (lookformedical.com)
Coagulation6
Protease-activated receptor 4 (PAR-4), also known as coagulation factor II (thrombin) receptor-like 3, is a protein that in humans is encoded by the F2RL3 gene. (wikipedia.org)
The matriptase inhibitor design in this thesis focused on matriptase-affine compounds with a fair selectivity profile against the blood coagulation enzymes thrombin and fXa. (uni-marburg.de)
7 The differential reductions in the potencies of SLIGRL-NH2, compared with trypsin in the PAR2PRR and PAR2NET cell lines point to differences between the interactions of the trypsin-revealed tethered ligand and the free receptor-activating peptide with PAR2. (nih.gov)
The receptor is activated by TRYPSIN, which cleaves off the N-terminal peptide from the receptor. (curehunter.com)
The uncleaved receptor can also be activated by the N-terminal peptide present on the activated THROMBIN RECEPTOR and by small synthetic peptides that contain the unmasked N-terminal sequence. (curehunter.com)
Protease2
Inter-alpha-trypsin inhibitor: emergence of a family within the Kunitz-type protease inhibitor superfamily. (embl.de)
Apixaban is a selective inhibitor of FXa, a serine trypsin-like protease. (affygility.com)
Specificity1
Furthermore, we used this assay to develop a PAR 1 receptor-activating probe [Ala-parafluoroPhe-Arg-Cha-Cit-Tyr-NH 2 (Cit-NH 2 )], which displays a high degree of specificity for PAR 1 over PAR 2 , and we used the assay to quantitate the ability of trypsin to disarm the activation of PAR 1 by thrombin. (aspetjournals.org)
Thrombin catalyzes enzymatic reactions that result in the formation of fibrin, the important binding part for blood clotting. (inteligand.com)
Pathway1
Recently we have studied the ability of an enzymatically modified form of LDL (E-LDL), obtained by treatment with trypsin and then cholesterol esterase, to activate C1, the complex that triggers activation of the classical pathway of complement. (europa.eu)
Clot2
Thrombin, which is generated at these phosphatidylserine sites, triggers the formation of fibrin fibers, which consolidate the platelet thrombus into a stable clot sealing the breach in a vessel wall ( 2 ). (frontiersin.org)
Thrombin, generated by reactions initiated by activated tissue factor VII/factor IX pathways, leads to clot formation. (medscape.com)
Contrast1
In contrast, the potency of trypsin in PAR2NET was almost the same as in wt-PAR2. (nih.gov)
Human1
Nanoparticles were produced by entrapping human thrombin (THR) into a sol-gel derived magnetite matrix followed by grinding to sizes below 200 nm and subsequent colloidization. (nature.com)
Complex1
The binding mode of this compound (compound 55) was crystallographically determined in complex with matriptase as well as trypsin. (uni-marburg.de)
Trypsin proved as a suitable alternative to matriptase for detailed binding mode analysis of the compounds N-terminus. (uni-marburg.de)
Vivo1
For instance, in previous studies a composite based on γ -Fe 2 O 3 nanoparticles decorated with a BSA coating and immobilized thrombin was described and used in in vivo study as a wound healing material 16 . (nature.com)
Fold1
But, the potency of trypsin was decreased by only 7 fold. (nih.gov)
Generation1
By inhibiting FXa, apixaban decreases thrombin generation and thrombus development which helps blood clots to form. (affygility.com)