• The MEROPS protease classification system counts 16 superfamilies (as of 2013) each containing many families. (wikipedia.org)
  • Trypsin is a member of the serine protease family. (recombinanttrypsin.com)
  • This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. (utsouthwestern.edu)
  • Kallikrein 11 (KLK11) is also known as Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP, which belongs to the peptidase S1 family and Kallikrein subfamily. (biovisi.com)
  • Kallikrein 11 /KLK11 is a possible multifunctional protease and efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. (biovisi.com)
  • Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. (marvelgent.com)
  • Enterokinase is a specific protease that cleaves at the carboxyl side of Lysine residue when it is preceded by four Aspartic acids (Asp-Asp-Asp-Asp-Lys) and is not followed by Proline. (marvelgent.com)
  • Endoproteinase Lys-C is a serine protease specific for cleavage of amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. (marvelgent.com)
  • ProteoSure™ Sequencing Grade Trypsin is a highly purified porcine trypsin variant that has been genetically improved, chemically modified for the highest autolysis-resistance and maximum specific tryptic activity in proteomic applications. (marvelgent.com)
  • Serine proteases fall into two broad categories based on their structure: chymotrypsin-like (trypsin-like) or subtilisin-like. (wikipedia.org)
  • The majority belong to the S1 family of the PA clan (superfamily) of proteases. (wikipedia.org)
  • For superfamilies, P: superfamily, containing a mixture of nucleophile class families, S: purely serine proteases. (wikipedia.org)
  • Within each superfamily, families are designated by their catalytic nucleophile, (S: serine proteases). (wikipedia.org)
  • The S1 pocket of chymotrypsin-like enzymes is more hydrophobic than in trypsin-like proteases. (wikipedia.org)
  • Elastase-like proteases have a much smaller S1 cleft than either trypsin- or chymotrypsin-like proteases. (wikipedia.org)
  • This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. (utsouthwestern.edu)
  • Serine proteases are grouped into clans that share structural homology and then further subgrouped into families that share close sequence homology. (bionity.com)
  • The three serine proteases of the chymotrypsin-like clan that have been studied in greatest detail are chymotrypsin , trypsin , and elastase . (bionity.com)
  • The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. (recombinanttrypsin.com)
  • Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0. (recombinanttrypsin.com)
  • Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container. (recombinanttrypsin.com)
  • 1)Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process. (recombinanttrypsin.com)
  • Using recombinant variants of ppFhCL3 we demonstrated the critical importance of a pair of propeptide residues (Tyr 46 Lys 47 ) for the interaction with the propeptide binding loop (PBL) of the mature enzyme and other residues (Leu 66 and Glu 68 ) that allow the propeptide to block the active site. (biomedcentral.com)
  • Therefore, the insulin glargine/Sul-β-CyD interaction or insulin glargine/SBE7-β-CyD complex is speculated to ameliorate the interaction between the negatively charged aspartic acid in the catalytic pocket of trypsin and positively charged lysine and/or arginines mentioned earlier, since Sul-β-CyD and SBE7-β-CyD have negative charge originating from the sulfate and sulfonate Inhibitors,research,lifescience,medical groups, respectively. (cyp17-signal.com)
  • 3)High purity: Higher specific activity.Host protein residues is less than the limits of biological products. (recombinanttrypsin.com)
  • For example, one of the predicted fragments of the human serum albumin protein is FKDLGEENFK (ends in lysine=K). This has an exact molecular mass of 1226.59 so if you find a peptide of that size in your mixture then it probably means that human serum albumin was present. (blogspot.com)
  • This can then interact with positively-charged residues such as arginine and lysine on the substrate peptide to be cleaved. (bionity.com)
  • Siglecs are a family of sialic acid-binding immunoglobulin-like receptors predominantly expressed on cells of immune system to regulate their functions through recognizing their glycan ligands [ 5 ]. (biomedcentral.com)
  • Results are presented that have been obtained by our group during the last four years concerning the expression of the glycoproteins human Interleukin 2 (Il-2) and Interferon-8 (IFN-8) in different mammalian cell lines, the determination of their carbohydrate residues, the effect of site-directed mutagenesis on their carbohydrate attachment sites and the insertion of peptide domains which function as acceptors for carbohydrates. (helmholtz-hzi.de)
  • Here, we identify in sperm of the freshwater fish Danio rerio a novel CNGK family member featuring non-canonical properties. (elifesciences.org)
  • HCV is a positive-strand RNA virus of the Flaviviridae family that contains 9.6 kb of RNA. (nature.com)