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  • INHIBITOR
  • The two predominant protease inhibitors of soybean seeds are the Kunitz trypsin inhibitor (KTI) and Bowman-Birk protease inhibitor (BBI). (usda.gov)
  • In addition to 7S and 11S seed storage proteins others known to protect the seeds against pathogens and pests including Kunitz trypsin inhibitor, peroxidase, a-galactosidase, and endo-1.3-ß-glucanase were also identified in the seed exudates. (usda.gov)
  • proteins
  • It competes with proteins to bind to trypsin and therefore renders it unavailable to bind with proteins for the digestion process. (wikipedia.org)
  • Long term incubation with high trypsin concentration damage cells by striping cell surface proteins and kill the cells. (sigmaaldrich.com)
  • The process is commonly referred to as trypsin proteolysis or trypsinisation, and proteins that have been digested/treated with trypsin are said to have been trypsinized. (wikipedia.org)
  • This means that trypsin predominantly cleaves proteins at the carboxyl side (or "C-terminal side") of the amino acids lysine and arginine except when either is bound to a C-terminal proline, although large-scale mass spectrometry data suggest cleavage occurs even with proline. (wikipedia.org)
  • These human genes encode proteins with trypsin enzymatic activity: Other isoforms of trypsin may also be found in other organisms. (wikipedia.org)
  • Enteropeptidase exhibits trypsin-like activity, cleaving proteins following a lysine at a specific cleavage site (Asp-Asp-Asp-Asp-Lys). (wikipedia.org)
  • cleaves
  • This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. (thermofisher.com)
  • autodigestion
  • This modern formulation of trypsin is stable at room temperature, but performs identically to traditional trypsin solutions that must be stored frozen at -20°C to prevent autodigestion. (sigmaaldrich.com)
  • tissue
  • Some people apply trypsin directly to wounds and ulcers to remove dead tissue and improve healing. (webmd.com)
  • Trypsin removes dead skin cells (tissue) and allows healthy tissue to grow. (webmd.com)
  • Applying trypsin to the skin seems to help remove dead tissue from wounds and improve healing. (webmd.com)
  • Hellgren L, Vincent J. Degradation and liquefication effect of streptokinase-streptodornase and stabilised trypsin on tissue necroses, crusts of fibrinoid, purulent exudate and clotted blood from leg ulcers. (webmd.com)
  • EDTA
  • 1. Bring the DPBS, the Trypsin-EDTA for Primary Cells, and the Trypsin Neutralizing Solution to room temperature before use. (atcc.org)
  • If the cell culture medium contains serum, each flask should be rinsed with DPBS twice prior to adding the Trypsin-EDTA for Primary Cells. (atcc.org)
  • 3. Using 1 to 2 mL for every 25 cm 2 , add the appropriate volume of trypsin-EDTA solution to each flask (e.g., each T-25 flask would be dissociated with 1 to 2 mL trypsin-EDTA). (atcc.org)
  • Gently pipette or swirl the culture to ensure all of the trypsin-EDTA solution has been neutralized. (atcc.org)
  • 9. Transfer the cell / DPBS suspension to the centrifuge tube containing the trypsin-EDTA-dissociated cells. (atcc.org)
  • Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots. (atcc.org)
  • enteropeptidase
  • As the pro-region of trypsinogen contains this sequence, enteropeptidase catalyses its activation in vivo: trypsinogentrypsin + pro-region (Val-Asp-Asp-Asp-Asp-Lys) In humans, enteropeptidase is encoded by the PRSS7 gene (also known as ENTK) on chromosome 21q21. (wikipedia.org)
  • amino
  • Trypsin is considered an endopeptidase, i.e., the cleavage occurs within the polypeptide chain rather than at the terminal amino acids located at the ends of polypeptides. (wikipedia.org)