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TRPM Cation ChannelsCationsIon ChannelsTRPC Cation ChannelsCalcium ChannelsTRPV Cation ChannelsIon Channel GatingMentholCations, DivalentTransient Receptor Potential ChannelsPotassium Channels, Inwardly RectifyingCyclic Nucleotide-Gated Cation ChannelsCalcium Channel BlockersCalciumCations, MonovalentPatch-Clamp TechniquesChloride ChannelsPotassium Channel BlockersMembrane PotentialsFlufenamic AcidElectrophysiologyPotassium Channels, Voltage-GatedMagnesiumCalcium Channels, L-TypeElectric ConductivityTRPP Cation ChannelsAcid Sensing Ion ChannelsThermosensingKATP ChannelsPotassium Channels, Calcium-ActivatedThermoreceptorsIon TransportPotassiumSodium Channel BlockersCell LineSodiumShaker Superfamily of Potassium ChannelsEpithelial Sodium ChannelsMembrane Transport ModulatorsOocytesCell MembraneMolecular Sequence DataCalcium Channels, N-TypeCells, CulturedAmino Acid SequenceXenopus laevisLarge-Conductance Calcium-Activated Potassium ChannelsCalcium Channels, T-TypeAdenosine TriphosphateHEK293 CellsKineticsHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsGadoliniumAntipruriticsBariumCold TemperatureCalcium Channel AgonistsBoron CompoundsCation Transport ProteinsRetinal Bipolar CellsXenopusNeuronsCalcium SignalingCell Membrane PermeabilityEther-A-Go-Go Potassium ChannelsAdenosine Diphosphate RiboseKv1.2 Potassium ChannelKv1.3 Potassium ChannelMutationDose-Response Relationship, DrugCapsaicinKv1.1 Potassium ChannelKv1.5 Potassium ChannelDegenerin Sodium ChannelsProtein Structure, TertiaryIonsHydrogen-Ion ConcentrationRats, Sprague-DawleyMembrane ProteinsAmilorideSmall-Conductance Calcium-Activated Potassium ChannelsTransfectionTetraethylammoniumKCNQ Potassium ChannelsCesiumPyrimidinonesShab Potassium ChannelsKCNQ1 Potassium ChannelOrganic Cation Transport ProteinsAction PotentialsChloridesReceptors, Purinergic P2XOrganic Cation Transporter 1Kv1.4 Potassium ChannelLanthanumSensory Receptor CellsBinding SitesModels, BiologicalGanglia, SpinalPhosphatidylinositol 4,5-Diphosphate
TRPM4SubfamilyTransientTRPM8TRPM3SerineMassive autophosphorylationBifunctionalMutationsMagnesium homeostasisProteinsReabsorptionProteinGeneCalciumAmino acidFamilyMechanismExpressionActivityPropertiesCellIdentifyMelastatinTRPM1MammalianTRPVKinaseIntracellularMagnesiumSubunitsInhibitionRegulationRegulatorExpressionElectrophysiologyMouseHumanFunctionalCellsFormCurrentSpecificIncludes
TRPM43
  • Among the functional responsibilities of the TRPM channels are: regulation of calcium oscillations after T cell activation and prevention of cardiac conduction disorders (TRPM4). (wikipedia.org)
  • RESULTS: Among channels that showed mechanosensitivity, significant amounts of TRPM7, TRPC1, and TRPM4 transcripts were found. (bvsalud.org)
  • CONCLUSIONS: Our findings suggest that TRPM7, TRPC1, and TRPM4 channels represent stretch-activated nonselective cation channels in rat ventricular myocytes. (bvsalud.org)
Subfamily3
  • The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. (ecmbio.com)
  • The TRPM Subfamily: TRPM1. (booksca.ca)
  • Violin plots show distribution of expression levels for Transient receptor potential cation channel subfamily M member-like 2 (SMED30003521) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
Transient3
  • TRPM is a family of transient receptor potential ion channels (M standing for wikt:melastatin). (wikipedia.org)
  • Transient receptor potential cation channels in disease" (PDF). (wikipedia.org)
  • The TRPM6 protein is a new member of the long transient receptor potential channel (TRPM) family and is highly similar to TRPM7 (also known as TRP-PLIK), a bifunctional protein that combines calcium- and magnesium-permeable cation channel properties with protein kinase activity. (tau.ac.il)
TRPM85
  • The TRPM family consists of eight different channels, TRPM1-TRPM8. (wikipedia.org)
  • In this work, we investigated the presence and function of TRPM8, a non-selective and cold-sensitive Ca2+-permeable ion channel in the primary microglia cell as well as in microglia cell line BV2. (bvsalud.org)
  • TRPM8 is a non-selective cation channel permeable to both monovalent and divalent cations that is activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. (bvsalud.org)
  • Here, we present a cryo-electron microscopy structure of a human TRPM8 channel in the closed state that was solved at 2.7 Å resolution. (bvsalud.org)
  • In selectivity assays englerin A weakly inhibits TRPA1, TRPV3/V4, and TRPM8 which suggests that englerin A may bind a common feature of TRP ion channels. (epfl.ch)
TRPM31
Serine2
  • In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. (ecmbio.com)
  • TRPM6 and TRPM7, for example, contain functional α-kinase segments, which are a type of serine/threonine-specific protein kinase. (wikipedia.org)
Massive autophosphorylation3
  • Here, we demonstrate that the cytosolic C-terminus of TRPM7 undergoes massive autophosphorylation (32+/-4 mol/mol), which strongly increases the rate of substrate phosphorylation. (prinsesmaximacentrum.nl)
  • In conclusion, our findings support a model where massive autophosphorylation outside the catalytic domain of TRPM6 and TRPM7 may facilitate kinase-substrate interactions leading to enhanced phosphorylation of those substrates. (prinsesmaximacentrum.nl)
  • TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. (ecmbio.com)
Bifunctional1
  • TRPM6 and TRPM7 are bifunctional proteins expressing a TRP channel fused to an atypical alpha-kinase domain. (prinsesmaximacentrum.nl)
Mutations1
  • Using a positional candidate gene approach, we identified mutations in TRPM6 (also known as CHAK2), encoding TRPM6, in autosomal-recessive hypomagnesemia with secondary hypocalcemia (HSH, OMIM 602014), previously mapped to chromosome 9q22 (ref. 3). (tau.ac.il)
Magnesium homeostasis2
  • TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. (ecmbio.com)
  • These findings indicate that TRPM6 is crucial for magnesium homeostasis and implicate a TRPM family member in human disease. (tau.ac.il)
Proteins1
  • Unlike the TRPC and TRPV sub-families, TRPM subunits do not contain N-terminal ankyrin repeat motifs but, rather, contain entire functional proteins in their C-termini. (wikipedia.org)
Reabsorption2
  • TRPV5, a Ca2 Channel for the Fine Tuning of Ca2 Reabsorption. (booksca.ca)
  • regulation of magnesium reabsorption in the kidneys and absorption in the intestines (TRPM6). (wikipedia.org)
Protein1
  • We also visualized several lipids that are bound by the protein and modeled how the human channel interacts with icilin. (bvsalud.org)
Gene1
  • The gene expression levels of various ion channels in freshly isolated rat ventricular cardiomyocytes were investigated using the RNA-seq technique. (bvsalud.org)
Calcium1
Amino acid2
  • TRPM7 (a.a. 1484-1497) synthetic peptide corresponding to amino acid 1484 to 1497 in human TRPM7. (ecmbio.com)
  • Analyses of pore helices in available TRPM structures showed that all these structures can be grouped into different closed, desensitized and open state conformations based on the register of the pore helix S6 which positions particular amino acid residues at the channel constriction. (bvsalud.org)
Family2
  • This site is not well conserved in rat and mouse TRPM7, and has no homology to other TRPM family members. (ecmbio.com)
  • However, since englerin A can be synthesized in the laboratory, it may be a useful chemical starting point to identify novel modulators of other TRP family channels. (epfl.ch)
Mechanism3
  • Recently, we found that TRPM7 associates with its substrate myosin II via a kinase-dependent mechanism suggesting a role for autophosphorylation in substrate recognition. (prinsesmaximacentrum.nl)
  • Surprisingly, the TRPM6-kinase is regulated by an analogous mechanism despite a lack of sequence conservation with the TRPM7 Ser/Thr-rich domain. (prinsesmaximacentrum.nl)
  • The mechanism of activation also varies greatly among TRPM channels. (wikipedia.org)
Expression2
  • TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. (ecmbio.com)
  • Expression of the TRPC4 cation channel was the cell line feature that best correlated with sensitivity to englerin A, suggesting the hypothesis that TRPC4 is the efficacy target for englerin A. Genetic experiments demonstrate that TRPC4 expression is both necessary and sufficient for englerin A induced growth inhibition. (epfl.ch)
Activity2
  • While the gating properties of TRPM6 and TRPM7 channels have been studied in detail, little is known about the mechanisms regulating kinase activity. (prinsesmaximacentrum.nl)
  • Due to the magnesium inhibition, TRPM7's ion channel activity is very low. (ecmbio.com)
Properties2
  • Since the response to Gd3+ was maintained in Cs+ in/Cs+ out solutions, we suggest that voltage-gated CaV1.2 channels in the ventricular myocytes of adult rats also exhibit mechanosensitive properties. (bvsalud.org)
  • Probably the CaV1.2 channels in these cells exhibit mechanosensitive properties. (bvsalud.org)
Cell5
  • regulation of cell adhesion (TRPM7). (wikipedia.org)
  • We also measured changes in current through CaV1.2 channels under cell stretching using the whole-cell patch-clamp method. (bvsalud.org)
  • The application of a nonspecific ISAC blocker, Gd3+, during cell stretching eliminated ISAC through nonselective cation channels and ICa,L through CaV1.2 channels. (bvsalud.org)
  • Englerin A Agonizes the TRPC4/C5 Cation Channels to Inhibit Tumor Cell Line Proliferation. (epfl.ch)
  • These experiments confirm that activation of TRPC4/C5 channels inhibits tumor cell line proliferation and confirms the TRPC4 target hypothesis generated by the cell line profiling. (epfl.ch)
Identify1
  • BACKGROUND: The study aimed to identify transcripts of specific ion channels in rat ventricular cardiomyocytes and determine their potential role in the regulation of ionic currents in response to mechanical stimulation. (bvsalud.org)
Melastatin5
  • The transient receptor potential melastatin type 6 (TRPM6) ion channel regulates the body Mg(2+) homeostasis by mediating transcellular Mg(2+) absorption in kidney and intestine. (nih.gov)
  • The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. (phosphosolutions.com)
  • TRPM is a family of transient receptor potential ion channels (M standing for wikt:melastatin). (wikipedia.org)
  • Currently, 28 different TRP channels have been identified in mammals and they are grouped by sequence similarity into several subfamilies: TRPC (canonical), TRPM (melastatin), TRPV (vanilloid), TRPA (ankyrin), TRPML (mucolipin), and TRPP (polycystin). (metrionbiosciences.com)
  • The transient receptor potential melastatin member 7 (TRPM7) and member 6 (TRPM6) are divalent cation channel kinases essential for magnesium (Mg2+) homeostasis in vertebrates. (antiviralbiologic.com)
TRPM11
  • The TRPM family consists of eight different channels, TRPM1-TRPM8. (wikipedia.org)
Mammalian1
  • Expression of P2X6, which does not form functional channels in mammalian cells, did not affect the function of TRPM6. (nih.gov)
TRPV1
  • Unlike the TRPC and TRPV sub-families, TRPM subunits do not contain N-terminal ankyrin repeat motifs but, rather, contain entire functional proteins in their C-termini. (wikipedia.org)
Kinase8
  • To identify the intracellular signaling molecules that mediate the P2X4-dependent inhibition of TRPM6, the cells were treated with inhibitors of protein kinase c, protein kinase a, and phosphoinositide 3-kinase. (nih.gov)
  • Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). (phosphosolutions.com)
  • In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. (phosphosolutions.com)
  • TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. (phosphosolutions.com)
  • This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells stimulated with calyculin A, and detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. (phosphosolutions.com)
  • TRPM6 and TRPM7, for example, contain functional α-kinase segments, which are a type of serine/threonine-specific protein kinase. (wikipedia.org)
  • tet-inducible HEK-293 cells encoding human + human WT (TRPM7/M6), human TRPM7 + human TRPM6 K1804R (TRPM7/M6 K1804R), and human TRPM7 + human TRPM6 kinase (TRPM7/M6 kinase) were maintained in DMEM containing 10% FBS, blasticidin (5 g/ml), Zeocin (0.4 mg/ml), and hygromycin (0.5 mg/ml) (17). (antiviralbiologic.com)
  • Current measurements of TRPM7/M6, TRPM7/M6 K1804R, and TRPM7/M6 kinase PCI-24781 were conducted in an extracellular solution composed of (in mm) the following: 140 NaCl, 2.8 KCl, 1 CaCl2, 2 MgCl2, 10 Hepes-NaOH, 11 glucose, pH 7.4, adjusted with NaOH or HCl. (antiviralbiologic.com)
Intracellular1
  • Activation of TRP channels leads to a plethora of signalling events, which includes membrane depolarisation through the influx of cations, activation of intracellular signalling pathways, initiation of endocytosis/exocytosis, etc. (metrionbiosciences.com)
Magnesium4
Subunits1
  • TRP channels are expected to be composed of four subunits surrounding a centrally located ion permeation pore. (metrionbiosciences.com)
Inhibition3
  • The inhibition was dependent on the activity of P2X4, since a P2X4 mutant with altered ATP sensitivity was not able to inhibit TRPM6. (nih.gov)
  • Additionally, P2X4 was unable to inhibit TRPM7, a close homologue of TRPM6, suggesting that the inhibition is specific for TRPM6. (nih.gov)
  • However, none of these inhibitors prevented the inhibition of TRPM6 by P2X4. (nih.gov)
Regulation1
Regulator2
  • Here, the P2X4 receptor was established as a novel regulator of TRPM6 activity. (nih.gov)
  • One of these voltage-gated ion channels, the hyperpolarization-activated cyclic nucleotide-gated (HCN) channel and its current I(h), is an important regulator of neuronal excitability. (epfl.ch)
Expression2
  • While P2x4 was highly expressed in the colon, both P2x4 and P2x6 mRNA were prominently expressed in the distal convoluted tubule segment of the kidney, a segment with high Trpm6 expression. (nih.gov)
  • TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. (phosphosolutions.com)
Electrophysiology1
Mouse1
  • This site is conserved in rat and mouse TRPM7, but the homology surrounding the site is low. (phosphosolutions.com)
Human3
  • Phospho-TRPM7 (Ser-1493) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1493 in human TRPM7. (phosphosolutions.com)
  • Tetracycline-inducible human TRPM7 HEK-293 cells were cultured in DMEM containing 10% FBS, blasticidin (5 g/ml), and Zeocin (0.4 mg/ml) (8). (antiviralbiologic.com)
  • Tetracycline-inducible human TRPM6 HEK-293 cells were maintained in DMEM containing 10% FBS, blasticidin (5 PCI-24781 g/ml), and hygromycin (0.5 mg/ml) (Invitrogen) (17). (antiviralbiologic.com)
Functional1
  • Functional TRPM channels are believed to form tetramers. (wikipedia.org)
Cells1
  • Solutions To measure TRPM6 currents, cells were kept in an extracellular solution containing (in mm) the following: 140 NaCl, 2.8 KCl, 1 CaCl2, 10 Hepes-NaOH, 11 glucose, pH 7.4. (antiviralbiologic.com)
Form1
  • The majority of TRP channels form homotetramers, but a large number are also able to form heterotetramers. (metrionbiosciences.com)
Current1
  • Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate. (epfl.ch)
Specific2
  • The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513), phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497), TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493), phospho-specific (lanes 5 & 6). (phosphosolutions.com)
  • What are your specific ion channel and assay needs? (metrionbiosciences.com)
Includes1
  • Metrion has developed compound screening assays for TRP channels, such as TRPV1 and TRPA1, on a variety of platforms, which includes plate based fluorescent readers, automated patch clamp (QPatch 48 and Patchliner) and manual patch clamp. (metrionbiosciences.com)