Trastuzumab antibody specificity. Medical search. Frequent questions

MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB9589N, filled histogram) or irrelevant antibody (open histogram). (rndsystems.com)
HERZUMA (trastuzumab-pkrb) is a humanized IgG1 kappa monoclonal antibody that selectively binds with high affinity to the extracellular domain of the human epidermal growth factor receptor 2 protein, HER2 . (rxlist.com)
Recombinant monoclonal antibody to trastuzumab (anti-idiotype). (absoluteantibody.com)
Overexpression of miR-21 decreases the sensitivity of gastric cancer cells to trastuzumab, which is a humanized monoclonal antibody targeting human epidermal growth factor receptor 2. (oncotarget.com)
These elicited antibodies impaired the high affinity interaction between VEGF and monoclonal antibody Bevacizumab, an antiangiogenic drug approved in combination with chemotherapy for the treatment of different tumors. (researchsquare.com)
So far, the most successful passive immunotherapy directed to VEGF is Bevacizumab, a monoclonal antibody that neutralizes the binding of VEGF to its receptors [5], which has been approved, in combination with chemotherapy, for the treatment of different tumors [6-12]. (researchsquare.com)
In 1975, Georges Köhler and César Milstein were the first to describe the creation of hybridomas for monoclonal antibody production. (labmanager.com)
Dr. Fox explains this process: "We receive the antigen from the person who wishes to develop a monoclonal antibody. (labmanager.com)
Supernatant [fluid] is taken from the hybridomas, and the investigator screens for the monoclonal antibody of interest that will recognize a specific antigen and is appropriate for their application. (labmanager.com)
Tositumomab is used in the treatment of some B-cell non-Hodgkin lymphomas and is an example of a monoclonal antibody used to deliver toxins, as some of the antibody delivers a linked radioactive substance to the B-cells. (labmanager.com)
A recombinant IgG1 kappa, humanized monoclonal antibody that selectively binds with high affinity in a cell-based assay (Kd = 5 nM) to the extracellular domain of the human epidermal growth factor receptor protein. (creativebiolabs.net)
The following product was used in this experiment: NK1.1 Monoclonal Antibody (PK136), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-5941-82, RRID AB_469159. (thermofisher.com)
Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. (thermofisher.com)
For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. (thermofisher.com)
Numerous monoclonal therapeutic and diagnostic monoclonal antibody (MAbs), are used in clinical settings to diagnose and treat human diseases such as cancer and autoimmune disorders. (patentpc.com)
One vial containing 420 mg of trastuzumab, a humanised IgG1 monoclonal antibody produced by mammalian (Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)
For example, Antibody-Drug Conjugates (ADCs) are a type of active targeting strategy that involves the conjugation of a monoclonal antibody with a cytotoxic drug. (scitechnol.com)
Cancer cells were treated with trastuzumab, a HER2-targeting monoclonal antibody, and then co-cultured with NK cells with our without anti-CD16. (axionbiosystems.com)

Herzuma (trastuzumab-pkrb) is biosimilar to Herceptin (trastuzumab). (rxlist.com)
Trastuzumab (Herceptin), for example, targets the HER2 protein on the surface of breast and stomach cells. (labmanager.com)
The level of insulin growth factor-1 receptor expression is directly correlated with the tumor uptake of (111)In-IGF-1(E3R) in vivo and the clonogenic survival of breast cancer cells exposed in vitro to trastuzumab (Herceptin). (ox.ac.uk)
Among the 1st restorative antibodies to straight focus on tumor had been anti-CD20 (Rituxan or Rituximab) and anti-Her2 (Herceptin or Trastuzumab) antibodies to deal with M cell leukemias and breasts tumor, respectively. (researchensemble.com)
For example, the patent expiration of the blockbuster drug Herceptin (trastuzumab), a widely used sterile injectable drug for breast cancer, has paved the way for the entry of generic versions, providing more affordable options for patients and driving the growth of the generic sterile injectable market. (outsourcedpharma.com)

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). (rndsystems.com)
This antibody can be used in flow cytometry or various assay formats to measure Her2 expression. (rndsystems.com)
Herzuma (trastuzumab-pkrb) is a HER2 /neu receptor antagonist indicated for the treatment of HER2-overexpressing breast cancer . (rxlist.com)
As noted by the investigators, margetuximab is a chimeric antibody that shares HER2 specificity with trastuzumab but also incorporates an engineered Fc region designed to increase immune activation. (ascopost.com)
This antibody recognises the idiotype of trastuzumab, mimicking HER2 epitopes. (absoluteantibody.com)
This antibody binds to the idiotype of trastuzumab mimicking HER2 epitopes. (absoluteantibody.com)
In a murine in vivo model this antibody was able to ellicit anti-HER2 antibodies which protected against HER2-positive SK-OV-3 tumour cells. (absoluteantibody.com)
Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV.f.huHER2(Fo5) mice. (absoluteantibody.com)
This study is a continuation of our earlier findings involving poly(ε-caprolactone) (PCL)-poly (ethylene glycol) (PEG) nanoparticles (PEG-PCL NPs), which were coated with trastuzumab to target gastric cancer cells with HER2 receptor over-expression using anti-miRNA-21 antisense oligonucleotides (AMO-21). (oncotarget.com)
We will engineer the Trastuzumab antibody to selectively bind its target, HER2, at low pH and to selectively interact with cancer killing immune cells at low pH, introducing complimentary mechanisms to improve specificity of an already effective breast cancer therapeutic. (texas.gov)
These findings demonstrated a promising detection specificity and treatment efficacy for our system (SiNPs-TZ, 1:8 Hc-TZ), encouraging its potential use as a new theranostic agent for HER2-positive BC lesions. (dovepress.com)
A second objective was to correlate IGF-1R (and HER2 density) with the cytotoxicity of trastuzumab in the absence/presence of IGFBP-3 or the IGF-1R tyrosine kinase inhibitor, AG1024. (ox.ac.uk)
Resistance of BC cells to trastuzumab was directly associated with IGF-1R expression, despite co-expression of HER2. (ox.ac.uk)
CONCLUSION: Imaging of IGF-1R expression using [(111)In]-IGF-1(E3R) may be useful for identifying HER2-positive tumors in BC patients that are resistant to trastuzumab through this mechanism. (ox.ac.uk)
For example, the anti-HER2 antibody trastuzumab has been used to treat HER2-positive breast cancer. (labmanager.com)
Breast cancer therapy has improved following the development of drugs with specific molecular targets, exemplified by inhibitors of human epidermal growth factor receptor-2 (HER2) or epidermal growth factor receptor (EGFR) such as trastuzumab and lapatinib. (jcancer.org)
In oncology, several targeted therapies have been approved for the treatment of various types of cancer, such as trastuzumab for HER2-positive breast cancer and imatinib for chronic myeloid leukemia. (scitechnol.com)
Analizar las diferencias en la presentación de variables clínico-patológicas, de acuerdo con la expresión proteica de GRB7, en tumores HER2 positivos en mujeres colombianas con cáncer de mama invasivo, diagnosticado entre los años 2013 y 2015 en el Instituto Nacional de Cancerología E.S.E (INC). Métodos. (bvsalud.org)
Se evaluó la expresión de los receptores hormonales (receptor de estrógeno (RE) y de progesterona (RP)), HER2, Ki67 y GRB7, mediante inmunohistoquímica (IHQ), y a partir de estos, se clasificaron los tumores en subtipos intrínsecos. (bvsalud.org)
Tumor Her2/ neu is generally assessed as protein overexpression by using immunohistochemistry (IHC), and patients with tumors that either have 2+ or 3+ results with this method become good candidates for treatment with trastuzumab. (biomedcentral.com)

The antibody conjugates (HER-PEG-PCL NPs) act against target cells via antibody-dependent mechanisms and also based on encapsutalated AMO-21. (oncotarget.com)
We found that the antibody conjugates significantly enhanced the cellular uptake of NPs. (oncotarget.com)
Antibody-drug conjugates (ADCs) distribute cytotoxic payloads to cells with the degree and specificity of cellular uptake being crucial to the molecule's effectiveness and safety. (news-medical.net)
Antibody-drug conjugates (ADCs) are a new type of targeted therapy which are very promising in cancer treatment that significantly increase target specificity as well as reduce toxcity. (eaglebio.com)
ADCs, antibody-drug conjugates, are a class of highly potent drugs for the treatment of cancer. (cis-pharma.com)
ARCs, antibody-radionuclide conjugates, are a form of radiotherapy, where a radionuclide molecule is linked to an antibody. (cis-pharma.com)
Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)

Despite their efficiency, adoptive T-cell therapies show unique toxicities, which are distinct from those seen with conventional chemotherapies, monoclonal antibodies (mAbs), and small-molecule-targeted therapies. (nature.com)
Therapeutic monoclonal antibodies (mAbs), for example, have emerged as a promising treatment strategy. (labmanager.com)
495-497, 1975), MAbs were hailed as the 'Magic Bullet' that harnessed the therapeutic power of specific antibodies against specific diseases. (inscientioveritas.org)
Selective compounds have been developed that target either the extracellular ligand-binding region of the EGFR (including a number of monoclonal antibodies [MAbs], immunotoxins, and ligand-binding cytotoxic agents) or the intracellular tyrosine kinase region (including various small-molecule inhibitors). (medscape.com)
Antibody-dependent cellular cytotoxicity (ADCC) involves the recognition of tumor antigens by monoclonal antibodies (mAbs), which leads to enhanced tumor cell killing by immune cells, specifically natural killer (NK) cells. (axionbiosystems.com)

In spite of the exquisite specificity and high affinity of antibody-based cancer therapies, they often cause damage to healthy cells since the cancer antigens are present on healthy and tumor cells. (texas.gov)
Monoclonal antibodies developed against immunogenic proteins (Tumor Specific Antigens/TSA's) that are expressed in human cancers, display a unique behavioral pattern. (jcancer.org)
Monoclonals developed from these tumor antigens are in the initial phases of investigation with regard to their specificity and antitumor activity. (jcancer.org)
Antibodies against tumor-associated epitopes, that are limited by antigens presented for the cell surface area of tumors, have already been exploited and determined against multiple types of malignancies using passive immunization.4 Notable for example rituximab (anti-CD20 for B-cell lymphomas5) and trastuzumab (anti-HER-2/neu for several breast malignancies6). (techieindex.net)
Antibodies targeting two tumor antigens and CD3 are being tested. (msdmanuals.com)
Zahavi and Weiner (2020) reviewed that monoclonal custom antibodies against antigens unique to cancer cells can cause tumor cell death by numerous mechanisms. (conductscience.com)

This increases the binding affinity and specificity compared to libraries of free peptides, though can limit the target repertoire of Affimers. (wikipedia.org)
The affinity of Trastuzumab (TP-083CL) was determined by SPR. (creativebiolabs.net)
Optionally, manufacturers can engineer the recombinant antibodies to have specific properties, such as higher binding affinity, improved stability, or the ability to target particular cell types (which is not possible with traditional antibodies). (labmanager.com)
The ability to precisely design and engineer recombinant antibodies with desired properties, such as increased affinity, specificity, and stability, makes them more versatile and valuable in different applications. (labmanager.com)
The affinity matured antibodies are more or less binding to a target antibody. (patentpc.com)
This article aims to offer methods for optimizing and enhancing the binding affiniites and selecting antibodies with desired affinity. (patentpc.com)
This document describes methods for affinity maturation of antibodies and fragments of them based on structure/activity relation (SAR). (patentpc.com)
The affinity matured starting antibody has a higher binding specificity and affinity than the target antigen. (patentpc.com)
This method entails affinity maturation of a portion of a first or secondary antibody for a target protein. (patentpc.com)
Treatment with these antibodies, realizing one particular antigen with a described affinity, offers underscored the restorative potential of really antigen-targeted immunotherapy, as amazing medical advantage offers been reported across research covering the last 10 years (3, 4). (researchensemble.com)

Some of the chimeric antibodies include rituximab and cetuximab. (conductscience.com)

Commercially available test antibodies included CD71 clone 1a, 2, CD20 (Sigma), clone 3-5 (Abcam), CD3, CD45 (Biolegend), human IgG isotype control (Absolute Antibody), mouse IgG1 isotype control (R&D Systems). (news-medical.net)
The IgG class of antibodies is further divided into four subclasses IgG1, IgG2, IgG3, and IgG4. (conductscience.com)

The antibody specifically binds to a target antigen on the surface of cancer cells, allowing for the selective delivery of the cytotoxic drug to the cancer cells while minimizing its interaction with normal cells [6-8]. (scitechnol.com)
T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)

Leinco Technologies is a leader in developing and manufacturing recombinant antibodies for early discovery research and diagnostics. (genestarbio.com.tw)

Additionally, the hereditary system of Testosterone levels cells 132539-06-1 with tumor-reactive TCRs (11, 12) or antibody-based chimeric antigen receptors (Vehicles) (13) provides obtained raising curiosity in latest years, and the initial scientific studies using adoptive transfer of such FLI1 gene-modified Testosterone levels cells possess showed powerful and long lasting anti-tumor replies in chosen sufferers (14C18). (researchensemble.com)
Chimeric antibodies prepared as a result comprised V L and V H from mice and C H1 , C H2 , and C H3 from the human. (conductscience.com)

The high cost can make recombinant antibody-based therapies inaccessible to many patients who need them. (labmanager.com)
At the same time, there was an increasing appreciation of the fact that, although antimicrobials work (and work well) by slowing down and/or killing the microbial pathogens, the totality of host defence is a dynamic process dependent upon host-pathogen interactions - because of which immune therapies, including antibody-associated therapies, had a definite and important role to play in clinical therapeutics. (inscientioveritas.org)
Not surprisingly, this situation resulted in a renewed interest in immune-based therapies, including antibody therapy, aimed at combating disease by buttressing host immunity. (inscientioveritas.org)
Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
NK cells kill target cells that have antibodies bound to their surface, making them useful for assessing antibody-based therapies. (axionbiosystems.com)
Cancer immunotherapies can be divided into four major categories: - Cytokines/immunomodulation agents - Monoclonal antibodies - Cell‑based therapies - Oncolytic viruses Though monoclonal antibodies currently represent the largest class of commercialized cancer immunotherapies, cell‑based therapies are rapidly making headway. (technologynetworks.com)

Stability measurements, cellular saturation binding assays, positron emission tomographic imaging, and biodistribution studies in mice bearing SK-OV-3 tumors confirmed the biochemical viability and tumor specificity of photoradiolabeled [ 89 Zr]ZrDFO-azepin-trastuzumab. (tau.ac.il)
Stability measurements, cellular saturation binding assays, positron emission tomographic imaging, and biodistribution studies in mice bearing SK-OV-3 tumors confirmed the biochemical viability and tumor specificity of photoradiolabeled [89Zr]ZrDFO-azepin-trastuzumab. (tau.ac.il)
These assays usually require labeling each antibody with a fluorescent tag, and in nearly all of these cases the labeled antibody must then be split from the free label via a wash step or column. (news-medical.net)
Eagle Biosciences has quickly become a leading provider of ELISA assay kits, HPLC assay kits, Molecular Biology assays, antibodies, and proteins. (eaglebio.com)

A recent study published in Molecular Cancer Therapeutics focused on evaluating the therapeutic potential of a new Antibody-drug conjugate (ADC), SYD985. (eaglebio.com)
Bubendorf, Switzerland, Tuesday, December 8, 2020 - CIS Pharma successfully completed the biodistribution study with its polymer carrier technology applied to an antibody radionuclide conjugate (ARC). (cis-pharma.com)
The biodistribution including the ratio of tumor to healthy tissue of the conjugate was similar to the Trastuzumab antibody labeled directly with lutecium-177 or indium-111. (cis-pharma.com)
CIS Pharma has developed a radionuclide-specific PDC that is optimized to conjugate radionuclides to an antibody. (cis-pharma.com)

They] can work by recruiting immune elements, delivering toxins, producing CAR-T cells, or as bispecific antibodies that recruit T or NK cells to kill the cancer cells. (labmanager.com)

NK cells were recovered from multiple donors, and the magnitude of cancer cytolysis and ADCC in the presence of trastuzumab were measured using the Maestro Z . (axionbiosystems.com)
NK-mediated cell killing and ADCC via trastuzumab were observed across all donors. (axionbiosystems.com)
Restorative antibodies experienced achievement against tumors, eliciting both complement-mediated reactions and antibody-dependent mobile cytotoxicity (ADCC). (techieindex.net)
The effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), structural factors, experience, and availability of IgG determine the preference of one type of immunoglobulin over another (Ryman and Meibohm, 2017). (conductscience.com)
Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. (cusabio.com)

X-ray photoelectron spectroscopy validated the presence of trastuzumab on NP surface. (oncotarget.com)

Phage display libraries of 109 randomized sequences are used to screen for Affimer proteins that exhibit high-specificity binding to the target protein with binding affinities in the nM range. (wikipedia.org)
In the biotechnology industry, scientists use recombinant antibodies to study protein structures and signal transduction pathways. (labmanager.com)
Antibodies are large heterodimeric protein molecules consisting of four polypeptide chains, i.e., two heavy chains of 50kDa each and two light chains of 25kDa. (conductscience.com)

ADCs are cell specific monoclonal antibodies that are conjugated with highly potent cyotoxics though a specific linker that help to deliver and release the drug inside the tumor cell. (eaglebio.com)
Conventional ADCs are composed of a chemotherapeutic drug that is conjugated directly to an antibody targeting cancer cells. (cis-pharma.com)

Multimeric forms of Affimers with the same target specificity provide avidity effects in target binding. (wikipedia.org)
Dr Christian Geraths, CSO of CIS Pharma AG, said: "These results clearly indicate that the antibody's target specificity is unimpaired when we attach our polymer carrier to it. (cis-pharma.com)

ELISA of anti-trastuzumab (anti-idiotype) antibody on trastuzumab antibody. (absoluteantibody.com)
Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (il-10.com)
Human IgG antibody Laboratories manufactures the crosss reactivity of pmsg and lh elisa testing reagents distributed by Genprice. (il-10.com)
Description: A competitive ELISA for quantitative measurement of Mouse Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (il-10.com)

Therapeutic antibodies are widely used for the treatment of diseases, including cancer and autoimmunity. (genestarbio.com.tw)
Research-grade biosimilar antibodies are produced by recombinant technology using the variable region sequences of therapeutic antibodies, resulting in the same specificities as approved biologics. (genestarbio.com.tw)

A perfect example of this phenomenon is the widely prescribed breast cancer antibody Trastuzumab, which has significant, well-documented cardiotoxic side effects in many patients because it binds a molecule found on the surface of certain breast cancer cells and also on heart cells. (texas.gov)

In summary, our data indicate that miR-205 could predict Trastuzumab efficacy and that its modulation might be useful as adjuvant treatment to improve the response to the drug. (oncotarget.com)
Her-2/neu and EGFR tyrosine kinase activation predict the efficacy of trastuzumab-based therapy in patients with metastatic breast cancer. (nanotools.de)

For the first time, there is experimental evidence of the presence of polyclonal antibodies directed to clinically relevant epitopes on VEGF. (researchsquare.com)
19 To many effectively funnel the billed power of T cells in the fight tumors, several methods have already been designed that allow T cells to react to traditional antibody epitopes. (techieindex.net)

For the past few decades, custom antibodies have been used to fight against autoimmune, cancerous, and infectious diseases. (conductscience.com)
With the technical advancements and an understanding of their molecular properties, custom antibodies have become the most important class of molecules in immunologic research. (conductscience.com)
Eventually, entirely human custom antibodies were prepared by employing either two technologies, i.e., phage display or transgenic mice (Ruker and Knopp. (conductscience.com)

Another example is the anti-EGFR antibody cetuximab, which is effective against colorectal and head and neck cancer. (labmanager.com)

The incidence and severity was highest in patients receiving trastuzumab with anthracycline-containing chemotherapy regimens. (rxlist.com)
This unique approach of engineering shapes of anti-cancer drugs and combining them with antibodies represents new direction in chemotherapy," Mitragotri added. (innovations-report.com)
Margetuximab plus chemotherapy improved progression-free survival vs trastuzumab plus chemotherapy in patients with pretreated ERBB2-positive advanced breast cancer. (ascopost.com)
Biocon Biologics Ltd), BT-ON014 - in combination with docetaxel for the treatment of those patients who have not received chemotherapy for their metastatic disease - in combination with an aromatase inhibitor for the treatment of postmenopausal patients with hormone-receptor positive MBC, not previously treated with trastuzumab. (who.int)
Although this challenge was somewhat resolved by the 1930s through better-purified antibodies, the introduction of vastly superior, potent, and better tolerated antimicrobial chemotherapy ('antibiotics', starting with sulphonamides and then the β-lactam, penicillin) around 1935 sounded the death-knell for serum therapy. (inscientioveritas.org)
Nevertheless, administration of the anti-cancer antibody like a monotherapy can be rare, and they are often coupled with even more traditional chemotherapy (evaluated in ref. 4). (techieindex.net)

Here we demonstrate that HER3 inhibition by miR-205 ectopic expression or siRNA-mediated silencing improves the responsiveness to Trastuzumab in vitro in HER2+ BC cell lines, and that this effect is exerted through impairment of AKT-mediated pathway. (oncotarget.com)
Recombinant antibodies are produced in vitro rather than by infecting living organisms. (labmanager.com)

Polyclonal antibody response was specific to VEGF, and showed no cross reactivity with other VEGF family members like VEGF-C and VEGF-D. Specific IgM, IgA and IgG antibodies detected in the serum of vaccinated patients were able to block the binding of VEGF to its receptors VEGFR1 and VEGFR2. (researchsquare.com)
Once there is sufficient antibody titer in the serum, the spleen is removed to create hybridomas by fusing spleen cells with a cell line. (labmanager.com)
Antibodies are then harvested from the animal's blood or serum and purified. (labmanager.com)
The earlier experiments on antibodies were based on the injection of serum of immunized animals into the diseased subjects. (conductscience.com)

Additionally, monoclonal antibodies are utilized within immunotherapy to flag tumor cells for immune cell clearance (e.g. (news-medical.net)
Although immunoglobulins are still utilized in medical practice for untargeted safety against virus-like attacks, such as in individuals with general B-cell insufficiencies, 132539-06-1 the actual cutting-edge in medical immunotherapy arrived with understanding the hereditary profile of described monoclonal antibodies. (researchensemble.com)
The medical achievement of these beginning providers offers in latest years led to the advancement and regulatory authorization of extra antibodies to focus on numerous malignancies (5), propelling antigen-specific antibody-based immunotherapy into mainstream malignancy treatment. (researchensemble.com)
Administration of exogenous antibodies constitutes passive humoral immunotherapy. (msdmanuals.com)

In addition, laboratories can produce an antigen-specific recombinant antibody in as few as eight weeks. (labmanager.com)

Elicited polyclonal antibody response in addition to its cross-reactivity with human VEGF, have also demonstrated in rats and monkeys its capacity to impair the binding of Bevacizumab to VEGF [15, 16], indicating the presence of antibodies that block binding sites on VEGF which have shown to be clinically relevant. (researchsquare.com)
IVIG is a polyclonal antibody preparation derived from healthy donors (whose blood contains antibodies recognizing diphtheria toxin, measles and polio virus at the minimum, as a result of direct exposure, or vaccination) ( 2 ). (inscientioveritas.org)

Leinco's recombinant biosimilar antibodies are ideal research tools as they are fast, inexpensive, manufactured in an animal free facility and formulated in sterile PBS buffer, free of additives or preservatives. (genestarbio.com.tw)

We have found our strategy greatly enhances the specificity of anti-cancer drugs to cancer cells. (innovations-report.com)
To engineer these high-specificity drugs, they formed rod-shaped nanoparticles from a chemotherapeutic drug, camptothecin, and coated them with an antibody called trastuzumab that is selective for certain types of cancer cells, including breast cancer. (innovations-report.com)
The antibody-coated camptothecin nanorods were 10,000-fold more effective than tratsuzumab alone and 10-fold more effective than camptothecin alone at inhibiting breast cancer cell growth. (innovations-report.com)
Trastuzumab is the standard treatment for HER2+ breast cancer (BC) patients, and even though it significantly improved their clinical outcome, 50% of them do not benefit from this drug and disease recurs, underlining the need of reliable predictive biomarkers and new therapeutic strategies. (oncotarget.com)
Therefore, the identification of new actors of estrogen signaling pathways should improve treatment specificity and thus overcome cancer drug resistance. (scirp.org)
The methods we develop for Trastuzumab will be transferrable to other tumor-targeting antibodies, allowing more precise control over the tissues impacted by cancer therapy. (texas.gov)
Monoclonal antibodies are commonly utilized as anti-inflammatory, anti-cancer and anti-viral therapeutic agents. (news-medical.net)
The broad and potent tumor-reactivity of innate-like T cells makes them valuable additions to current cancer immunotherapeutic concepts based on adaptive immunity, such as monoclonal T and antibodies cells. (researchensemble.com)
1). Therefore considerably, the huge bulk of initiatives focused at making use of the resistant program to decline cancer tumor have got concentrated on elements of adaptive defenses, including monoclonal P and antibodies cells. (researchensemble.com)
Alternatively, the identical antibody-polymer-drug-carrier-system is loaded in a first step with imaging radio-isotopes to then load in a second run therapeutic radio-isotopes to treat cancer. (cis-pharma.com)
Different ratios of cancer and NK cells were combined together in the presence or absence of trastuzumab, and the efficiency of immune cell killing was measured using the Maestro Z . (axionbiosystems.com)

Reconstitution with 20 mL of the appropriate diluent (BWFI or SWFI) yields a solution containing 21 mg/mL trastuzumab-pkrb that delivers 20 mL (420 mg trastuzumab-pkrb), at a pH of approximately 6. (rxlist.com)
The bacteriostatic water for injection is a colourless, transparent solution, free of visible particles and is intended to use for the reconstitution of [BT-ON014 trade name] to yield a multi-dose solution containing 21 mg/mL trastuzumab at a pH of approximately 6.0. (who.int)

In traditional antibody production, researchers immunize animals with the target antigen to stimulate antibody production. (labmanager.com)
Unlike traditional antibody production, they do not exhibit genetic drift, antibody expression variation, and antibody sequence mutation that can cause non-specific binding. (labmanager.com)

Trastuzumab-pkrb is produced by recombinant DNA technology in a mammalian cell (Chinese Hamster Ovary) culture. (rxlist.com)

Trastuzumab F(ab')2 fragments. (absoluteantibody.com)
Incucyte ® FabFluor Antibody Labeling Reagents are Fc-region targeting Fab fragments that are conjugated to a pH-sensitive fluorescent probe. (news-medical.net)

The Core offers antibody development services for preclinical antibody research and investigation, using the same process involved in therapeutic mAb development. (labmanager.com)

We assessed Her-2/ neu alteration using CISH on formalin-fixed paraffin-embedded primary invasive ductal carcinoma tumors in which IHC (CB11 antibody) had previously been performed, and we compared the results with IHC. (biomedcentral.com)
It is a necessary adjunct in determining the subset of non-amplified IHC-positive invasive tumors that will not benefit from trastuzumab therapy. (biomedcentral.com)

This white paper outlines an approach developed to address these limitations: an integrated assay solution that is based on Incucyte ® Live-Cell Analysis, along with a novel pH-sensitive dye coupled antibody fragment (Incucyte ® FabFluor Antibody Labeling Reagents). (news-medical.net)
These reagents allow a generic single-step, no-wash, labeling protocol for the whole range of isotype matched, Fc-containing test antibodies. (news-medical.net)

CIGB-247 was immunogenic in a broader patient population, and induced Bevacizumab-like polyclonal antibodies, indicating that the VEGF-specific antibody response could have a potentially relevant clinical activity. (researchsquare.com)

These engineered non-antibody binding proteins are designed to mimic the molecular recognition characteristics of monoclonal antibodies in different applications. (wikipedia.org)
Affimer proteins display two peptide loops that can all be randomized to bind to desired target proteins, in a similar manner to monoclonal antibodies. (wikipedia.org)
Performance of Optimized Wide Pore Superficially Porous Particles for Separation of Proteins and Immunoglobulin G Antibodies. (creativebiolabs.net)

It is required by FDA that the potential effects of tumor control and toxicity be defined using the naked antibodies produced under GMP conditions, In those situations where patients with recurrent malignancies are to be studied we have come to realize that a number of factors can influence the response to monoclonal therapy. (jcancer.org)

The cell line specificity, cellular uptake, AMO-21 delivery, and cytotoxicity of the HER-PEG-PCL NPs were investigated. (oncotarget.com)

This may represent advantages over antibodies in terms of tissue penetration, for example in solid tumours where Avacta are developing PD-L1 inhibitors as alternatives to Opdivo and Yervoy, though requires half life modification to prevent rapid excretion through the kidney. (wikipedia.org)
We aim to protect cardiac tissue from the off-target effects of Trastuzumab therapy by taking advantage of the unique low pH environment near tumor cells. (texas.gov)
This includes antibodies that have the ability to modulate a target's functional activity and/or antibodies that are more bioavailable or stable in specific cellular and tissue environments. (patentpc.com)

A microtiter plate was coated overnight with pembrolizumab or human IgG4-Pro, human PD-1, nivolumab, natalizumab or trastuzumab at a concentration of 5 µg/ml. (bio-rad-antibodies.com)

To evaluate antibody-dependent cellular cytotoxicity using different effector:target (E:T) cell ratios. (axionbiosystems.com)

These synthetic antibodies were engineered to be stable, non-toxic, biologically neutral and contain no post-translational modifications or disulfide bridges. (wikipedia.org)
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed a stable antibody expression. (oncotarget.com)
Moreover, recombinant antibodies are genetically stable. (labmanager.com)

After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human Anti-Pembrolizumab Antibody, clone AbD30685_hIgG1 ( HCA297 ) at a concentration of 2 μg/ml in HISPEC Assay Diluent ( BUF049A ) followed by QuantaBlu Fluorogenic Peroxidase Substrate. (bio-rad-antibodies.com)
After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human Anti-Pembrolizumab Antibody, clone AbD30685_hIgG1 ( HCA297 ) titrated to the given concentrations in HISPEC Assay Diluent ( BUF049A ) and QuantaBlu Fluorogenic Peroxidase Substrate. (bio-rad-antibodies.com)
A microtiter plate was coated overnight with Human Anti-Pembrolizumab Antibody, clone AbD30689 ( HCA296 ) at a concentration of 1 µg/ml. (bio-rad-antibodies.com)

It is equally important to be able to compare the uptake of different antibodies in antibody selection, as this allows for these to be optimized for biologics drug discovery. (news-medical.net)
However, therapeutic-grade biologics are expensive, challenging to source, and only available in large quantities, making studies using these antibodies cost prohibited. (genestarbio.com.tw)

Anatomy12

Organisms11

Diseases14

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment73

Phenomena and Processes48

Disciplines and Occupations2

Information Science4

Health Care16

Monoclonal Antibody18

Herceptin5

Her220

Conjugates7

MAbs5

Antigens6

Affinity10

Rituximab1

IgG12

Cytotoxic2

Developing and manufacturing recombin1

Chimeric2

Therapies6

Assays4

Conjugate4

Bispecific1

ADCC5

Presence of trastuzumab1

Protein3

ADCs2

Target specificity2

ELISA4

Therapeutic antibodies2

Binds1

Efficacy2

Epitopes2

Custom Antibodies3

Cetuximab1

Chemotherapy6

Vitro2

Serum4

Immunotherapy4

Antigen-specific1

Polyclonal antibody2

Biosimilar Antibodies1

Cancer11

Solution co2

Traditional antibody2

Recombinant DNA techno1

Fragments2

Preclinical1

Tumors2

Reagents2

Bevacizumab1

Proteins3

Malignancies1

Cellular uptake1

Tissue3

Nivolumab1

Effector1

Stable3

Clone3

Biologics2