Transposon system cell lines and transgenic. Medical search. Frequent questions

For the design of the construct we combined the transmembrane domain of the EGF-receptor with a hemagglutinin epitope and a biotin acceptor peptide and performed a transposon-mediated transfection of myeloma cell lines. (hereon.de)
Transfection of this vector with those that encode Tol2 transposase and gRNA allows ubiquitous, tissue-specific, or conditional control of gene inactivation in zebrafish for production of stable mutant lines. (vectorbuilder.kr)
Conventional transfection or electroporation results in almost entirely transient delivery of DNA into host cells due to the loss of DNA over time. (vectorbuilder.kr)
Efficient integration of functional genes is an essential prerequisite for successful gene delivery such as cell transfection, animal transgenesis, and gene therapy. (tamu.edu)
The piggyBac (pB) transposase in particular has been shown to be well suited for cell transfection and gene therapy approaches because of its flexibility for molecular modification, large cargo capacity, and high transposition activity. (tamu.edu)

During transposition, the PiggyBac™ transposase recognises transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. (bioscience.co.uk)
The Sleeping Beauty transposase system was developed in the late 1990s and has been widely used in a range of vertebrate systems. (biomedcentral.com)
Once integrated into the frog genome, the 'cut-and-paste' DNA transposons are targets for remobilization by re-expression of the appropriate transposase enzyme. (biomedcentral.com)
The system consists of a PiggyBac Vector and the Super PiggyBac Transposase which recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. (reportergene.com)
The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors. (reportergene.com)
Footprint-free removal that leaves no PiggyBac sequences behind -In addition to ease-of-use, consistency, and the lack of limits on DNA insert size, what sets this system apart is the ability to reverse the integration reaction in a footprint-free way-with the Excision Only PiggyBac Transposase (Cat. (reportergene.com)
Tol2 technology enables the generation of transgenic zebrafish by transposase-mediated insertion of target genes into the genome of zebrafish embryos at random sites. (vectorbuilder.kr)
At each insertion site, the Tol2 transposase creates an 8 bp duplication, resulting in identical direct repeats flanking each transposon integration site in the genome. (vectorbuilder.kr)
Co-injection of this vector DNA with the helper plasmid coding Tol2 transposase (or transposase IVT mRNA) and a vector coding for your gRNA (or IVT-made gRNA) into fertilized eggs allows generation of stable zebrafish lines with ubiquitous, tissue-specific, or conditional heritable gene knockout. (vectorbuilder.kr)
To utilize P transposon-mediated insertion, the pUASTB plasmid and a P transposase-expressing helper plasmid are co-introduced into host cells or embryos. (vectorbuilder.cn)
The single-plasmid gene delivery system carries both the piggyBac transposase (pBt) expression cassette as well as the transposon cargo flanked by terminal repeat element sequences. (tamu.edu)

Current trends and future prospects for optimization of excision-activation machinery and its practical implementation for the generation of transgenic plants and plant products free of undesired genes are discussed. (bioone.org)

The high fecundity of the frog combined with the ability to remobilize transposon transgenes integrated into frog genome will allow large-scale insertional mutagenesis screens to be performed in laboratories with modest husbandry capacities. (biomedcentral.com)
The closely related frog Xenopus tropicalis shares all the features of X. laevis that make this system useful for embryonic manipulation but it develops more rapidly (sexual maturity is reached in 5 to 9 months) and has a diploid genome. (biomedcentral.com)
Transposons have widely been used in plant and invertebrate model species to integrate foreign DNA into the host genome. (biomedcentral.com)
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. (reportergene.com)
In this genome-editing system, the Cas9 enzyme forms a complex with a guide RNA (gRNA), which provides targeting specificity through direct interaction with complementary 18-22 nt target sequences in the genome. (vectorbuilder.kr)
Mintz participated in the development of transgenic mice by the incorporation of foreign DNA into a mouse genome. (asu.edu)
Recent decoding of genome sequences of various animals since the turn of the century has promoted genome sciences and systems biology as the next revolution in the biological sciences. (oist.jp)
Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. (encyclopedia.pub)
Among them, the large family of transposons first described by Barbara McClintock in maize [ 5 ] have the ability to move their genetic information within the genome. (encyclopedia.pub)
In contrast, Subclass II transposons, such as members of the Helitron superfamily [ 9 ] , follow a copy-and-paste mechanism, during which the element generates copies of itself which integrate into the genome. (encyclopedia.pub)
Our Drosophila Cas9 expression pUASTB vector system has the capability to utilize either the Drosophila P-element transposon system (like pUAST) or the bacteriophage φC31 integration system (like pUASTattB) for Cas9 insertion into the genome. (vectorbuilder.cn)
For this reason, the attB vector system is designed to be used with Drosophila lines carrying attP "landing sites" within their genome. (vectorbuilder.cn)
The use of site-specific recombination to remove undesired DNA from the plant genome and concomitantly, via excision-mediated DNA rearrangement, switch-activate by choice transgenes of agronomical, food or feed quality traits provides a versatile "transgene maintenance and control" strategy that can significantly contribute to the transfer of transgenic laboratory developments into farming practice. (bioone.org)
With these and other changes, Badgett predicted that a 3 Gb genome could be assembled de novo with the Sequel System for $9,000 by next year and as little as $1,500 in 2018. (pacb.com)
The recent controversy over the transgenic contamination of the Mexican landraces [10] hinges on observations suggesting that the transgenic DNA with the CaMV 35S promoter is "fragmenting and promiscuously scattering throughout the genome" of the landraces, observations that would be consistent with our expectations [11]. (i-sis.org.uk)
TEs are able to move throughout the genome either directly (DNA transposons) or by an RNA intermediate (Retrotransposons). (prolekarniky.cz)

The TTAA-specific transposon PiggyBac™ is a mobile genetic element that efficiently transposes between vectors and chromosomes via a 'cut and paste' mechanism. (bioscience.co.uk)
Transposon vectors can be used in the frog for transgenesis and for insertional mutagenesis where enhancer trap and gene trap constructs are used to identify genomic loci involved in developmental processes. (biomedcentral.com)
Consistent and easy-to-use, SBI's PiggyBac Transposon System includes cloning and expression vectors that come with a range of markers as well as both constitutive and inducible promoters. (reportergene.com)
Utilizing the CRISPR/Cas9 system in zebrafish allows for the rapid generation of knockout lines by simply delivering either an all-in-one vector (a single vector expressing both Cas9 and gRNA) or separate vectors for driving Cas9 and gRNA expression. (vectorbuilder.kr)

In contrast, through Tol2 transposition, injection of the transposon plasmid along with the helper plasmid (or introduction of Tol2 mRNA) in the fertilized eggs may result in a permanent genomic integration during early stages of embryonic development in many cells. (vectorbuilder.kr)
These approaches include cotransformation with two independent T-DNAs or plasmid DNAs followed by their subsequent segregation, transposon-mediated DNA elimination, and most recently, attempts to replace bacterial T-DNA borders and selectable marker genes with functional equivalents of plant origin. (bioone.org)
Improvements to the helper-independent structure were achieved by developing new plasmids in which the pBt gene is rendered inactive after excision of the transposon from the plasmid. (tamu.edu)
A pathogenic bacterium of plants that can inject a plasmid DNA (T DNA) into plant cells. (nationalacademies.org)

Most techniques for genetic manipulation, including random mutagenesis with a gene trap (retroviral-based and non-retroviral-based), gene knock-outs/knock-ins, and conditional mutations, depend upon the culture and manipulation of embryonic stem (ES) cells. (wikipedia.org)
The rapid accumulation of genetic resources for this animal and the success of pilot mutagenesis screens have helped propel this model system forward. (biomedcentral.com)
Genetic manipulation of this tractable model system would further enhance the use of the frog in developmental studies. (biomedcentral.com)
not, we recorded by view the mass of a reset design of the Stochastic Approximation Expectation-Maximization unit in Monolix to transfer the outcomes of a observed analysis where PSA systems were acquired by a corresponding genetic diameter antisense. (scoutconnection.com)
Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. (cshlpress.com)
Researchers later determined that the genetic mismatch was due to chimerism, a condition in which two genetically distinct cell lines are present in one body. (asu.edu)
Most cells have a total of 46 autosomes, or non-sex chromosomes, and a pair sex chromosomes, XX for genetic females, or XY for genetic males. (asu.edu)
Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. (encyclopedia.pub)
Genetic dissection of maize seedling root system architecture traits using an ultra-high density bin-map and a recombinant inbred line population [J]. J Integr Plant Biol. (jipb.net)
The homing endonuclease (HEG) gene drive system is one proposed genetic strategy [2] . (plos.org)
It is designed to cross species barriers and to jump into genomes, and it has homologies to the DNA of many species and their genetic parasites (plasmids, transposons and viruses), thereby enhancing recombination with all of them [2]. (i-sis.org.uk)
The risk of cancer is highlighted by the recent report that gene therapy - genetic modification of human cells - claimed its first cancer victim [4]. (i-sis.org.uk)
The transgenic constructs used in genetic modification are basically the same whether it is of human cells or of other animals and plants. (i-sis.org.uk)
Animals (or embryos) composed of cells of different genetic origin. (nationalacademies.org)
The contributors describe strategies for producing genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using transposon-based insertional mutagenesis, RNA interference, viral-mediated gene delivery, and chemical carcinogens. (cshlpress.com)

Close transposon relatives and other recombination enzymes, including retroviral integrases, have served as useful models to infer functional information relevant to SB. (encyclopedia.pub)

These integration systems are ideally suited to transgenesis and insertional mutagenesis strategies in the frog. (biomedcentral.com)
Easy, consistent transgenesis with no limits on cargo size -For transgenesis that's easy, consistent, and not limited by cargo size, SBI's PiggyBac Transposon System is an excellent choice. (reportergene.com)

The results presented herein demonstrate that our helper-independent plasmids represent an important step in the development of safe and efficient gene delivery methods that should prove valuable in gene therapy and transgenic approaches. (tamu.edu)
In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. (upenn.edu)

GDF15 promotes the generation of peripherally derived inducible Treg (iTreg) cells and enhances the suppressive function of natural Treg (nTreg) cells by interacting with a previously unrecognized receptor CD48 on T cells and thus downregulates STUB1, an E3 ligase that mediates forkhead box P3 (FOXP3) protein degradation. (bmj.com)
This system also incorporates a strong Gal4-inducible promoter to regulate Cas9 gene expression. (vectorbuilder.cn)
In this pUASTB system, Cas9 gene is cloned downstream of an engineered, inducible promoter consisting of five tandemly arrayed GAL4 binding sites (5xUAS) and the hsp70 TATA box promoter. (vectorbuilder.cn)

Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
Contrary to expectations, a fragment containing the entire region between the TBPH gene and the bgcn translational start drove strong HEG expression only during late spermatogenesis rather than in the germline stem cells and spermatogonia as expected. (plos.org)

Recently, interest in ZmWUS 's stem cell promoting properties has resurfaced due to their use in efficient transformation systems for maize and other recalcitrant plant species 12 , 13 . (nature.com)
The Dslox transposable element system is an enabling technology that combines the advantages of transposon-tagging using the maize Ac/Ds system to produce insertion mutants, but it also includes a lox sequence in the Ds element and another strategically placed upstream but within the T-DNA. (ttu.edu)
We developed a pair of maize near-isogenic lines (NILs) with different alleles for a starch quantitative trait locus on chromosome 3 ( qHS3 ), resulting in different kernel starch content. (jipb.net)

For further information about this vector system, please refer to the papers below. (vectorbuilder.kr)

Driven by the need for efficient transposon-based gene vector systems, extensive studies have largely elucidated the molecular actors and actions taking place during SB transposition. (encyclopedia.pub)
We anticipate that continued investigations into the structure-function relationships of SB transposition will enable the development of new generations of transposition-based vector systems, thereby facilitating the use of SB in preclinical studies and clinical trials. (encyclopedia.pub)
Class II transposons are DNA transposons solely relying on DNA intermediates in their transposition process. (encyclopedia.pub)
In contrast to transposon-based systems, such as P-element-mediated transposition, φC31-mediated insertion is irreversible. (vectorbuilder.cn)

Cis effect of lacZ sequences in transgenic mice. (shengsci.com)
Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. (shengsci.com)
Skeletal defects in Osterix-Cre transgenic mice. (shengsci.com)
She has worked with various cancers and produced viable mice from the cells of a teratoma. (asu.edu)
We used flow cytometry, coimmunoprecipitation, RNA sequencing, mass spectrum, chromatin immunoprecipitation and Gdf15 -/- , OT-I and GFP transgenic mice to demonstrate the effects of GDF15 on Treg cells and related molecular mechanism. (bmj.com)

The frog Xenopus laevis has been an important model of vertebrate cell biology and development for many decades. (biomedcentral.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
Plant Cell Biotechnology and Molecular Biology , 15 (1-2), 90-110. (ttu.edu)

By stacking longitudinal Work at home gums, fibrillar network leverages, left parasitic cells, or following misconfigured scalable sequences thus, the health and paper of stable clones of factors could log removed. (scoutconnection.com)
dedicated ConclusionsAn properties in the soluble competing epub are digested on the been strategy sequences that are data of the Bacterial application as effects in the cell for the bacteriophage. (naturheilpraxis-gisbert-fussek.de)

The CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9) system has greatly facilitated inactivation of genes in vitro and in vivo in a wide range of organisms. (vectorbuilder.kr)
Taking advantage of this simple mode of embryogenesis, as well as well-characterized genes involved in developmental, Ciona is now a model experimental system for exploring gene regulatory networks for differentiation of embryonic cells [Imai et al. (oist.jp)
Recently, several groups have used transposon-based approaches to deliver genes to a variety of cells. (tamu.edu)
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
Transgenic constructs contain new combinations of genes that have never existed, and they also amplify gene products that have never been part of our food chain [3]. (i-sis.org.uk)
Like TAK1 overexpressing cardiac cells, cardiomyocytes differentiated in the presence of OXO beat faster and showed increased expression of SAN genes (Shox2, HCN4, and Islet1). (bvsalud.org)

Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. (frontiersin.org)
The procedure, in which bone marrow cells are genetically modified outside the body and re-implanted, was previously thought to avoid creating infectious viruses and causing cancer, both recognized major hazards of gene therapy. (i-sis.org.uk)

Stem cells reside at the growing tip of meristems, where they differentiate to produce new organs throughout the life of plant and maintain a constant reservoir of pluripotent stem cells. (nature.com)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Chemical conversion of human conventional Pluripotent Stem Cells to Trophoblast Stem Cells. (axonmedchem.com)
Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)

This can also be achieved by directly injecting gRNA and Cas9 in vitro transcribed (IVT) mRNA into one-cell stage embryos. (vectorbuilder.kr)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
Establishment in culture of pluripotential cells from mouse embryos. (springer.com)
Cell lines derived from early embryos that have the potential to differentiate into all types of somatic cells as well as to form germ line cells, and hence whole animals, when injected into early embryos. (nationalacademies.org)

We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. (shengsci.com)
These Cells generate that at some modeling after the promoter and single pitch focused biased, the fact power treated a copy focus in the longitudinal way but as in the additional construct. (scoutconnection.com)
An aggressive promoter from a virus is often used to boost the expression of the transgene, in animal and human cells, from the cytomegalovirus that infects mammalian cells, and in plants, the 35S promoter from the cauliflower mosaic virus (CaMV) that infects Cruciferae plants. (i-sis.org.uk)
Unfortunately, although the CaMV virus is specific for plants, its 35S promoter is active in species across the living world, human cells included, as we discovered in the scientific literature dating back to 1989. (i-sis.org.uk)

This review highlights diverse strategies to deliver CRISPR/Cas gene editing reagents to regenerable plant cells and to recover edited plants without unwanted integration of transgenes. (frontiersin.org)

Only later, the epidermis exhibits enhanced cell proliferation. (biologists.com)

Stem cell maintenance in the shoot is under the control of the CLAVATA-WUSCHEL (CLV-WUS) negative feedback-loop, which is tightly integrated with hormone function, in particular auxin and cytokinin that promote cell differentiation and proliferation, respectively 2 . (nature.com)
Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19. (axonmedchem.com)
Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
Epigenetic regulation of gene expression is an essential mechanism that guides cell differentiation during development. (elifesciences.org)
To understand its role in SAN differentiation, clonal embryonic stem (ES) cell lines were made that conditionally overexpress Tbx5, Tbx3, Tbx18, Shox2, Islet-1, and MAP3k7/TAK1. (bvsalud.org)
EBs overexpressing Tbx5, Islet1, and TAK1 beat faster than cardiac cells differentiated from control ES cell lines, suggesting possible roles in SAN differentiation. (bvsalud.org)

Although cloning by somatic cell nuclear transfer (SCNT) could theoretically be used to create rats with specific mutations by mutating somatic cells, and then using these cells for SCNT, this approach has not been used successfully to create knockout rats. (wikipedia.org)
This would result in many many mutations as plant genomes are easily in the range of 1,000,000,000 Bases (Gbases) Name some sources of mistakes which could eventually lead to mutations: tautomeric structures of DNA bases cell internal metabolites, e.g. reactive oxygen species external chemicals e.g. alkylation agents radiation e.g. (usadellab.org)
the creation of artificial mutations by transposon mutagenesis was investigated. (ttu.edu)

These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)

Among these are traits that depend on functional meristems, populations of stem cells maintained by the CLAVATA-WUSCHEL (CLV-WUS) negative feedback-loop that controls the expression of the WUS homeobox transcription factor. (nature.com)
The control of plant stem cells is essential for sustaining the function of apical meristems, plant growth, and ultimately productivity 1 . (nature.com)
WUS is a homeodomain transcription factor (TF) produced in the organizing center (OC) domain of apical meristems and is transported via plasmodesmata into the apical domain (called central zone, CZ) to promote proliferation of stem cells 3 . (nature.com)
All specialized cells originate from stem cells. (benthamscience.com)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
Stem cells and regenerative medicine - future perspectives. (springer.com)
Stem cells in regenerative medicine: introduction. (springer.com)

Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
Humankind's radically increased ability to deliver precise genotypes in crops thanks to advances in breeding, transformation, transgenics, and editing is helping growers keep pace with increased demand for food and energy. (frontiersin.org)
I have been drawing our regulators' attention to it at least since 1996 [1], when there was already sufficient evidence to suggest that transgenic DNA in GM crops and products can spread by being taken up directly by viruses and bacteria as well as plant and animals cells. (i-sis.org.uk)
By bridging historically siloed ecological, agronomic, and evolutionary lines of inquiry into feral crops, there is the opportunity to improve food security and understand this relatively understudied anthropogenic phenomenon. (weizmann.ac.il)

Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)

The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. (axonmedchem.com)
Embryonic stem cell lines derived from human blastocysts. (springer.com)
Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
To examine this phenomenon, we chose two mouse embryonic stem (ES) cell lines that possess insertionally polymorphic retrotransposons (IAP, ETn/MusD, and LINE elements) at specific loci in one cell line but not the other. (prolekarniky.cz)

Subclass I follows a cut-and-paste mechanism, during which the transposon is excised from one genomic location and reintegrates somewhere else [ 6 ] . (encyclopedia.pub)
We investigated the selective mechanism exploited by HCC that lead to Treg cells expansion and to find more efficacious immunotherapies. (bmj.com)
Conclusions Our results reveal the generation and function enhancement of Treg cells induced by GDF15 is a new mechanism for HCC-related immunosuppression. (bmj.com)
CD48 is the first discovered receptor of GDF15 in the immune system which provide the possibility to solve the molecular mechanism of the immunomodulatory function of GDF15. (bmj.com)

Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis. (shengsci.com)
HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)

Sleeping Beauty Transposon System" Encyclopedia , https://encyclopedia.pub/entry/6797 (accessed December 02, 2023). (encyclopedia.pub)

The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)

ETHNOPHARMACOLOGICAL RELEVANCE: ß-tubulin is a skeletal protein of sinoatrial node cells (SANCs) that maintains the physiological structure of SANCs and inhibits calcium overload. (bvsalud.org)

Those Dslox and Ac lines were characterized at the molecular level to permit sorting into single full insertion lines. (ttu.edu)

Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology. (reportergene.com)
Children also conducted research on chemical batteries called voltaic cells and briefly studied and manufactured gunpowder. (asu.edu)
Similarly, I was not surprised by the research results released earlier this year by the Food Standards Agency [12], indicating that transgenic DNA from GM soya flour, eaten in a single hamburger and milk shake meal, was found transferred to the bacteria in the gut contents from the colostomy bags of human volunteers. (i-sis.org.uk)

Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
i) The virulence of the biocontrol agent must be enhanced to overcome evolutionary barriers either by mixing with synergistic chemicals or with one or more organisms, and/or by mutagenic or transgenic enhancing of virulence of the biocontrol fungus. (weizmann.ac.il)

Knockout rat models can also complement existing transgenic mouse models. (wikipedia.org)
Complement system. (entrance-exam.net)

The consumption of milk from transgenic goats producing human lysozyme (hLZ) in their milk therefore has the potential to positively impact intestinal health. (shengsci.com)
The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (springer.com)

Ochmann, M.T. Sleeping Beauty Transposon System. (encyclopedia.pub)

Results GDF15 is positively associated with the elevation of Treg cell frequencies in patients wih HCC. (bmj.com)

Cambridge Bioscience offers access to the PiggyBac Transposon Vector Construction Services through a partnership between System Biosciences and Transposagen. (bioscience.co.uk)

Viable offspring derived from fetal and adult mammalian cells. (springer.com)

In contrast, class I transposons move in a copy-and-paste manner. (vectorbuilder.kr)
In contrast, such heterochromatin is not induced by LINE copies and only by a minority of polymorphic ETn/MusD copies. (prolekarniky.cz)

SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity. (axonmedchem.com)

In this study, L-selectin was found to be phosphorylated in lymphoblastoid cell lines, and phosphorylation was enhanced by phorbol ester (phorbol 12-myristate 13-acetate (PMA)) treatment. (upenn.edu)
Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR , thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. (sdbonline.org)

IVF refers to a medical procedure in which scientists inseminate an egg cell with a sperm cell outside of the body, such as in a glass dish in a clinical setting. (asu.edu)

Transplantation of cells, tissues, or organs from another member of the same species. (nationalacademies.org)

Fourth, each cell of the developing frog embryo contains yolk platelets that provide nutrition during prefeeding stages of embryonic life. (biomedcentral.com)

Molecular Cell , 82 (1), 190-208. (axonmedchem.com)
Molecular cell, 82(1), 106-122. (axonmedchem.com)

Immune system. (entrance-exam.net)
Cells of the immune system: T, B and macrophages. (entrance-exam.net)
What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
Immune cells that infiltrate lesions are important for atherosclerosis progression and immunotherapies. (biomedcentral.com)

A fertilized egg of Ciona intestinalis develops within 18 hours to a larva, which consists of approximately 2600 cells with distinct cell types, including epidermis, nervous system, muscle, notochord, mesenchyme, and endoderm. (oist.jp)

The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)

Anatomy16

Organisms12

Diseases5

Chemicals and Drugs27

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes57

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Transfection5

Transposase11

Generation of transgenic1

Genome16

Vectors4

Plasmid4

Genetic15

Recombination1

Transgenesis2

Plasmids2

Inducible3

Germline2

Maize3

Vector System1

Transposition4

Mice5

Biology3

Sequences2

Genes6

Genetically2

Pluripotent stem5

Embryos6

Promoter4

Transgenes1

Enhanced cell proliferation1

Differentiation7

Mutations3

Nonviral1

Stem cells10

Crops4

Approaches1

Embryonic stem4

Mechanism4

Proliferation3

Https1

Frogs2

Skeletal1

Insertion1

Research3

Organisms2

Complement2

Intestinal2

Sleeping Beauty1

Positively1

Biosciences1

Mammalian cells1

Contrast2

Chromatin1

Phosphorylation2

Clinical1

Species1

Stages of embryonic1

Molecular2

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Breakthrough1