Transposon resolvases. Medical search. Frequent questions

In composite transposons, the additional genes are flanked by insertion sequences, which supply the transposase. (microbiologynotes.org)
Structures of homologous composite transposons carrying cbaABC genes from Europe and North America. (sciepub.com)

Most of the isolates (20 of 30) hybridized to probes encoding regions of the transposase (tnpA) and resolvase (tnpR) genes from Tn501 and Tn21. (lincoln.ac.uk)
They are characterized by the presence of 35-48-bp terminal inverted repeats (IR), transposase (tnpA) and resolvase (tnpR) genes, a res-internal resolution site, and genes other than those required for transposition [14]. (dadospdf.com)
Once integrated into the frog genome, the 'cut-and-paste' DNA transposons are targets for remobilization by re-expression of the appropriate transposase enzyme. (biomedcentral.com)
The recombinase used by a specific mobile genetic element may be called an integrase , resolvase , or transposase . (microbiologynotes.org)
The flanking IS elements encode the transposase used by the transposon to move. (microbiologynotes.org)

Insertion sequences have two major characteristics: they are small relative to other transposable elements (generally around 700 to 2500 bp in length) and only code for proteins implicated in the transposition activity (they are thus different from other transposons, which also carry accessory genes such as antibiotic resistance genes). (wikipedia.org)
in a composite transposon, two insertion sequences flank one or more accessory genes, such as an antibiotic resistance gene (e.g. (wikipedia.org)
59:4024-4030, 1993) indicates the presence of hybrid transposons and provides evidence for extensive recombination, both between transposon genes and between transposon and mer genes, within these natural populations of bacteria. (lincoln.ac.uk)
Tn5083 and Tn5084 are recombinants, and are comprised of fragments from several transposons including Tn5085, and a relative of a putative transposon from B. firmus (which contains similar genes to the cadmium resistance operon of Staphylococcus aureus), as well as others. (dadospdf.com)
Some transposons bear transfer genes and can easily move between bacteria during the process of conjugation, They are called conjugative transposons or integrative conjugative elements (ICEs) . (microbiologynotes.org)
Unit transposons contain additional genes (e.g., antibiotic-resistance genes) in addition to the recombinases that enable them to transpose. (microbiologynotes.org)

Nevertheless, there exist another sort of transposons, called unit transposons, that do not carry insertion sequences at their extremities (e.g. (wikipedia.org)
A complex transposon does not rely on flanking insertion sequences for resolvase. (wikipedia.org)
Unit transposons are not associated with insertion sequences. (microbiologynotes.org)

Other transposons lack IS elements and encode their own transposition enzymes. (microbiologynotes.org)

The resolvase is part of the tns genome and cuts at flanking inverted repeats. (wikipedia.org)

The high fecundity of the frog combined with the ability to remobilize transposon transgenes integrated into frog genome will allow large-scale insertional mutagenesis screens to be performed in laboratories with modest husbandry capacities. (biomedcentral.com)
Transposons have widely been used in plant and invertebrate model species to integrate foreign DNA into the host genome. (biomedcentral.com)

We have shown that the mer determinant can either be located at the chromosome, or on a plasmid in the Bacillus species, and is carried by class II mercury resistance transposons: Tn5084 from B. cereus RC607 and B. cereus VKM684 (ATCC10702) and Tn5085 from Exiguobacterium sp. (dadospdf.com)
The majority of mercury resistance transposons that have been studied are class II. (dadospdf.com)
152 (2001) 503-514 been described: TnMERI1 from Bacillus megaterium MB1, which is a class II transposon [20, 21]. (dadospdf.com)
Analysis of the genetic context of bla KPC has revealed that this gene is mostly localized into a class 2 transposon named Tn 4401 ( 12 ). (cdc.gov)

Solution structure of the catalytic domain of gammadelta resolvase. (edu.pl)
Transposons are more complex in structure than IS elements. (microbiologynotes.org)

Transposon Resolvases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)

The enzymes then catalyze the double strand cleavage, exchange of double strands and rejoining of DNA helices so that the DNA transposon is formed into a circular PLASMID. (ucdenver.edu)