Transport various substrates nucleotide transport proteins. Medical search. Frequent questions

2009 identified 21 families of these substrate capture proteins, each with a different specificity predicted by genome context analyses. (tcdb.org)
UCP3's properties, similar to its excessive homology to different mitochondrial carriers, particularly to UCP2, its quick lifetime and low specificity of UCP3 antibodies , have hindered progress in understanding its organic operate and transport mechanism over many years. (aabioetica.org)
In addition, each TRAPP complex has distinct large subunits that determine the specificity of each complex towards its substrate Rab and are essential for activity in vivo. (cam.ac.uk)
The NBDs in ABCG2 remain in contact with one another even without a bound substrate, providing greater substrate specificity as the entrance to the transporter is not as globular as other ABC transporters like ABCB1 or ABCC1. (proteopedia.org)
Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. (stjohnslabs.com)
1973. Substrate-specificity and toxicological significance of pyrethroid-hydrolyzing esterases of mouse liver microsomes. (cdc.gov)

We have revealed the design principles and functions of "pH sensors" described as endogenous proteins regulated within the cellular pH range, including guanine nucleotide exchange factors regulating cell polarity (Frantz et al. (ucsf.edu)

2023 ). (1) the BioMNY complex with ATPase (BioM) and transmembrane protein (BioN). (tcdb.org)
Some transporters have a conserved transmembrane protein and two nucleotide binding proteins similar to those of ABC transporters. (tcdb.org)
Possibly, these two transmembrane proteins comprise the pathway for transmembrane transport. (tcdb.org)
Cystic fibrosis is also associated with cystic fibrosis transmembrane conductance regulator (CFTCR) gene mutation results in the abnormal expression of cystic fibrosis transmembrane conductance regulator (CFTCR) protein, which is a cyclic adenosine monophosphate (cAMP)-activated chloride channel present on the surface of many types of epithelial cells. (nursingpaperslayers.com)
These systems are comprised of transmembrane domains, nucleotide binding domains, and in most bacterial uptake systems, periplasmic binding proteins (PBPs) which transfer the ligand to the extracellular gate of the transmembrane domains. (unl.edu)

Chen, Z.S., Lee, K. and Kruh, G.D. (2001) Transport of Cyclic Nucleotides and Estradiol 17-Beta-d-glucuronide by Multidrug Resistance Protein 4. (scirp.org)

One protein from the obligate intracellular bacterial parasite, Rickettsia prowazekii, is of 498 amino acyl residues, and is believed to span the membrane 12 times. (wikipedia.org)
Since it has an amine-reactive succinimidyl group, fluorescein derived from CFSE can covalently bond to proteins or other amino groups in the cell or on the cell membrane. (dojindo.com)
Fluorescent dyes with aromatic amino or guanidine groups, such as propidium iodide (PI), diaminophenylindole (DAPI), acridine orange (AO), and Hoechst dyes, interact with nucleotides to emit fluorescence. (dojindo.com)
Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
In addition to its role in glucose metabolism, this pathway also regulates the redirection of free amino acids to protein synthesis via the mTOR-signaling pathway. (hindawi.com)
The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. (molvis.org)
In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
LysM, a small globular domain with approximately 40 amino acids, is a widespread protein module involved in binding peptidoglycan in bacteria and chitin in eukaryotes. (unl.edu)
ABC-type transporters couple ATP hydrolysis with the uptake and efflux of a wide range of substrates across bacterial membranes, including amino acids, peptides, lipids and sterols, and various drugs. (unl.edu)
Those authors did not include rRNA or tRNA genes, and they recognized that the basic substrate transport machinery could not be clearly defined, even though this minimal cell would rely greatly on the import of several substrates, including all 20 amino acids (for which it had no biosynthetic ability). (creation.com)
preferentially, post-translational amino-acid JmjC increases are activated provided and conserved to regulate pentose cell polysaccharides with antimicrobial program protein and Clot positions. (erik-mill.de)
Glucose is the major substrate for ATP synthesis through glycolysis and oxidative phosphorylation (OXPHOS), whereas intermediary metabolism through the tricarboxylic acid (TCA) cycle utilizes non-glucose-derived monocarboxylates, amino acids, and alpha ketoacids to support mitochondrial ATP and GTP synthesis. (molvis.org)
Our work bridges protein electrostatics and structure with cell biology to reveal how pHi dynamics regulates cell behaviors through protonation of titrating amino acids as a post-translational modification to regulate protein structure and function (Schönichen et al. (ucsf.edu)
Synthetic peptide taken within amino acid region 430-480 on human Rac protein kinase-alpha protein. (stjohnslabs.com)

It is important in oxidative phosphorylation, glycolysis, cell replication, nucleotide metabolism and protein biosynthesis. (newcastlelaboratories.com)
and energetic and intermediary metabolism (glycolysis, proton motive force generation, pentose phosphate pathway, lipid metabolism, and biosynthesis of nucleotides and cofactors). (creation.com)
However, glucose-6-phosphate is also the substrate for glycolysis and produces lactate. (medscape.com)
The immense increase in the intracellular phosphorylated intermediate compounds of glycolysis concurrently inhibits rephosphorylation of adenine nucleotides, activating the nucleic acid degradation pathway and resulting in increased uric acid, the end product. (medscape.com)

Magnesium is an essential co‐factor in the formation of the substrates of many enzymes and an activator of many e. (newcastlelaboratories.com)
Magnesium is an essential co‐factor in the formation of the substrates of many enzymes and an activator of many enzyme systems. (newcastlelaboratories.com)
The mode of action of enzymes and macromolecular complexes as protein machines will be investigated. (manchester.ac.uk)
Moreover, the evolutionary relationship of useful proteins and enzymes in bioremediation processes are identifiable across different species. (projectguru.in)
The domain was originally identified in enzymes that degrade bacterial cell walls, but proteins involved in many other biological functions also contain this domain. (unl.edu)
Our goals were twofold: 1) to characterize the gene expression, protein expression, and activity of key synthesizing and regulating enzymes of energy metabolism in the whole mouse retina, retinal compartments, and/or cells and 2) to provide an integrative analysis of the results related to function. (molvis.org)
In laboratory studies, neither molnupiravir nor N4-hydroxycytidine (NHC, the active moiety of molnupiravir) induced or inhibited major drug metabolising enzymes or major drug transporting systems. (who.int)

2017). Moreover, Golgi-associated Rab29 can recruit LRRK2 to the surface of the Golgi and activate it there for both auto- and Rab substrate phosphorylation. (stanford.edu)
This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. (stjohnslabs.com)
Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. (stjohnslabs.com)

BioMNY proteins are considered to constitute tripartite biotin transporters in prokaryotes. (tcdb.org)
However, unlike typical ABC transporters (E.I. Sun & M.H. Saier, unpublished results), they use small integral membrane proteins that are postulated to capture specific substrates. (tcdb.org)
ABC (ATP binding cassette) transporters catalyze the transport of a wide variety of molecules across lipid bilayers into or out of cells and organelles. (rug.nl)
One of the ABC transporters that is studied in detail is the osmoregulatory ABC transporter OpuA (class D). Osmotic control of OpuA involves gating by intracellular ionic strength and is mediated by lipid-protein interactions. (rug.nl)
A structural genomics approach is used to determine to the structures of proteins belonging to other classes of ABC transporters. (rug.nl)
Heide van der T., and Poolman, B. (2002) ABC transporters: one, two or four extracytoplasmic substrate-binding sites? (rug.nl)
This ability to exploit structure within a pre-protein is an unexplored area of protein transport, which may apply to other protein transporters, such as those of the endoplasmic reticulum and mitochondria. (elifesciences.org)
These transporters, many of which are sodium-coupled, have been shown to use an elevator mechanism of transport, but exactly how substrate binding is coupled to sodium ion binding and transport is not clear. (elifesciences.org)
The manuscript represents an important contribution to an ongoing discussion about the substrate binding site and mechanism of the Bile Acid Sodium Symporter (BASS) family of transporters. (elifesciences.org)
[2] ABCG2 belongs to the family of 48 transporter proteins called ATP-binding cassette transporters (ABC transporters). (proteopedia.org)
A high percentage of the ABC family transporters (19 of the 48) transport chemotherapeutic agents out of cells, making expression levels of ABC transporters a major indicator of cancer treatment prognosis. (proteopedia.org)
However, the limited set of chromatophore-encoded solute transporters appears insufficient for supporting metabolic connectivity or protein import. (frontiersin.org)
Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. (frontiersin.org)

How does crowding affect mobility of proteins and lipids? (rug.nl)

The results demonstrate the utility of the two-color MRP1 construct for investigating ATP-binding cassette transporter structural dynamics, and it holds great promise for high-throughput screening of chemical libraries for unknown activators, inhibitors, or transportable substrates of MRP1. (sdstate.edu)
periplasmic binding domain of ATP-binding cassette transporter-like systems that belong to the type 1 periplasmic binding fold protein superfamily. (unl.edu)
Breast cancer resistance protein (BCRP) is a half-molecule ATP-binding cassette transporter that forms a functional homodimer and pumps out various anticancer agents, such as 7-ethyl-10-hydroxycamptothecin, topotecan, mitoxantrone and flavopiridol, from cells. (elsevierpure.com)
In summary, both modulators of BCRP and functional single nucleotide polymorphisms within the BCRP gene affect the transporter function of the protein and thus can modulate drug sensitivity and substrate pharmacokinetics and pharmacodynamics in affected cells and individuals. (elsevierpure.com)
Here, we solve the crystal structure at 2.3 Å of a transporter from Neisseria meningitidis (ASBT NM ) in complex with pantoate, a potential substrate of ASBT NM . (elifesciences.org)
The ABCG2 multidrug transporter or abc subfamily G member 2 is a membrane protein from the A TP- B inding C assette (ABC) transporter family, specifically the G-subfamily. (proteopedia.org)
As an ABC Transporter , ABCG2 exhibits ATPase activity and uses the energy of ATP hydrolysis to facilitate transport. (proteopedia.org)
One molecule of ATP is hydrolyzed to transport substrates across the cell membrane while the second molecule of ATP is hydrolyzed to reset the transporter to its inward-facing conformation. (proteopedia.org)
The overall shift from inward-facing to outward-facing promotes the transport of substrates through the transporter. (proteopedia.org)
After substrates bind in Cavity 1, ATP binds each NBD leading to the transporter shifting from inward-facing to outward-facing. (proteopedia.org)

They are working to determine how such proteins interact with nucleic acids and partner proteins, and how they use ATP to drive the architectural changes required for catalysis and physical movement. (hopkinsmedicine.org)

Biochemical assays revealed that the BioM, N, and Y proteins form stable complexes in membranes of the heterologous host. (tcdb.org)
Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. (elifesciences.org)
The encapsulation and compartmentalisation of cells has necessitated the evolution of machineries that conduct proteins across membranes, including for protein secretion and organellar import. (elifesciences.org)
Investigation of UCP3 reconstituted in planar bilayer membranes revealed that it transports protons solely when activated by fatty acids (FA). (aabioetica.org)
The most commonly stained cell components are cell membranes, proteins, and nucleotides. (dojindo.com)
These compounds are neutral molecules that pass through cell membranes and covalently conjugate with cell proteins. (dojindo.com)
The bile acid sodium symporter (BASS) family transports a wide array of molecules across membranes, including bile acids in humans, and small metabolites in plants. (elifesciences.org)
These proteins harness the sodium ion gradient to transport bile acids across the plasma membranes of enterocytes of the terminal ileum and hepatocytes, respectively. (elifesciences.org)
It imports hundreds of nucleus-encoded proteins, and diverse metabolites are continuously exchanged across the two chromatophore envelope membranes. (frontiersin.org)

It is caused by a point mutation (G→T) at nucleotide position 559 of the gene, bovine solute carrier family 35 member 3 (SLC35A3). (researchgate.net)
Expression of truncated bio transport operons, each with one gene deleted, resulted in stable BioMN complexes but revealed only low amounts of BioMY and BioNY aggregates in the absence of the respective third partner. (tcdb.org)
Furthermore, gene overexpression is also frequently associated to antifungal resistance as well as an increase in the activity of proteins that reduce oxidative damage. (intechopen.com)
CABYR is a polymorphic calcium-binding protein of the sperm fibrous sheath (FS) which gene contains two coding regions (CR-A and CR-B) and is tyrosine as well as serine/threonine phosphorylated during in vitro sperm capacitation. (biomedcentral.com)
The uncoupling protein 1 ( UCP1 ) gene is thought to be extremely expressed in brown adipose tissue (BAT) that capabilities in thermogenesis. (aabioetica.org)
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
In Library, lysine-10( H3K9) vision gene has required a green protein-protein for visual studies and lacks visually one of the unknown conditions known with acid( Peters et al. (erik-mill.de)
Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. (frontiersin.org)
Apparently these chromatophore-targeted proteins evolved convergently to plastid-targeted expression regulators and are likely involved in gene expression control in the chromatophore. (frontiersin.org)
One of the few antigens against which mammals develop protective immunity is the highly polymorphic OspC protein, encoded by the ospC gene on the cp26 plasmid. (cdc.gov)
By means of DNA sequencing of a large sample collection of the pathogen from across the United States, we studied the gene for the bacterium's highly diverse OspC protein, protective immunity against which develops in animals. (cdc.gov)

These fatty acids are transported to the liver, where they are used for triglyceride synthesis and are exported as very-low-density lipoprotein (VLDL), which is elevated in these patients. (medscape.com)

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. (stjohnslabs.com)

D. & E. Uptake systems with two or four covalently linked substrate-binding domains (SBDs). (rug.nl)
These proteins contain a very tightly, sometimes covalently bound flavin nucleotide, either FMN (or) FAD. (biochemden.com)

Many bioY genes are located at loci encoding biotin biosynthesis or are unlinked to either biotin synthesis or other biotin transport genes. (tcdb.org)

The TMD is responsible for binding and transporting substrates, is embedded in the cell membrane, extends into the extracellular region (Figure 1). (proteopedia.org)
which is occluded when the protein in is the inward-facing conformation, is now open to the extracellular space and able to release the substrate. (proteopedia.org)

Surprisingly, the sequence analysis of PCR product also revealed the presence of two previously unknown homozygous mutations (TG→CT) at nucleotide positions 554 and 555 in addition to the previously reported heterozygous mutation at position 559. (researchgate.net)
Finally, the signal sequence is cleaved and the pre-protein is either folded or trafficked onwards. (elifesciences.org)
Available at: https://www.projectguru.in/multiple-sequence-alignment-studies-mera-protein-sequence/ [Accessed 03 Dec. 2023]. (projectguru.in)
Kapagunta, Chandrika, and Priya Chetty "Multiple sequence alignment studies of merA protein sequence. (projectguru.in)
In this article, the protein sequence of merA enzyme is studied with respect to its closely related sequences found in NCBI database, through Multiple Sequence Alignment (MSA) . (projectguru.in)
The purpose of multiple sequence alignment is to characterize protein families. (projectguru.in)
It also determines the consensus sequence of the aligned sequences and reveals biological facts about proteins. (projectguru.in)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)

Both cidofovir and adefovir are nucleoside phosphonate analogs, a class of novel antivirals structurally related to natural nucleotides (Fig. 1 ). (aspetjournals.org)
Yet risks of renal failure and renal replacement therapy were similar in patients treated with nucleoside analogues versus nucleotide analogues in real-life setting. (medscape.com)
These agents are classified according to their chemical structures: the three nucleoside analogues include lamivudine, telbivudine and entecavir, whereas the two nucleotide analogues include adefovir dipivoxil, tenofovir disoproxil fumarate (TDF), [ 2 ] and the newly approved tenofovir alafenamide (TAF). (medscape.com)

Absence of extracytoplasmic solute-binding proteins among the members of this group, however, is a distinctive feature. (tcdb.org)
When the intestinal barrier is healthy, it allows selective paracellular transport of nutrients, regulating solute and water fluxes while preventing the entry of bacteria and toxins. (nature.com)

When evidence suggests that homologous membrane transport proteins of the ABC2 type couple transport to ATP hydrolysis using a homologue of the ABC-type ATPases, we list these proteins in the ABC superfamily. (tcdb.org)
The transport reaction catalyzed by the antiporters is: ATP (out) + ADP (in) ⇌ ATP (in) + ADP (out) The AAA family proteins are distantly related to members of the major facilitator superfamily, and are not related to the mitochondrial ATP/ADP exchangers of the mitochondrial carrier family which pump ATP out of mitochondria in accordance with the polarity of the mitochondrial membrane potential. (wikipedia.org)

The long term goal of our research is to elucidate the molecular mechanisms by which proteins are targeted to specific and distinct compartments. (stanford.edu)
Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
Structural and biochemical analysis of a bacterial homolog, ASTBnm, in complex with its native substrate (not bile acids, but a vitamin A precursor, pantoate) show a new binding site that is consistent with classical proposals for elevator-type transport mechanisms. (elifesciences.org)

A molecular understanding of membrane traffic has broad implications for our understanding of growth control in cancer, receptor trafficking errors in heart disease, regulation of insulin secretion in diabetes and synaptic vesicle biogenesis and transport in neurological disorders. (stanford.edu)
In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion. (elifesciences.org)
The bulk of protein secretion and membrane protein insertion is conducted by the ubiquitous Sec translocon. (elifesciences.org)
They act in both protein secretion and autophagy and have also been proposed to have a role in other processes such as cytokinesis and ciliogenesis. (cam.ac.uk)

Membrane uncoupling protein 3 (UCP3), a member of the mitochondrial uncoupling protein household, was found in 1997. (aabioetica.org)
The Cytochromes of type a and b and some of type "C" are integral proteins of the inner mitochondrial membrane. (biochemden.com)

In 2006, "follow-up research led by Hamilton Smith at the J. Craig Venter Institute in Rockville reveals that the minimum genome consists of 387 protein-coding and 43 RNA-coding genes. (creation.com)

1996 for mercury phytoremediation, with respect to its homologous protein sequences. (projectguru.in)
Members of this group include ABC-type Leucine-Isoleucine-Valine-Binding Proteins (LIVBP), which are homologous to the aliphatic amidase transcriptional repressor, AmiC, of Pseudomonas aeruginosa. (unl.edu)

3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)

The Transport Protein Particle (TRAPP) complexes are highly conserved multisubunit complexes that act as nucleotide exchange factors (GEFs) for Rab GTPases. (cam.ac.uk)
The approach relies on a blend of structural, biochemical, and biophysical methods to define the architecture, function, evolution, and regulation of protein/nucleic acid complexes. (hopkinsmedicine.org)

CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)

We investigate the mobility of proteins in the bacterial cytoplasm and cytoplasmic membrane as well as membrane model systems, using optical microscopy-based techniques such as fluorescence correlation spectroscopy (FCS) and pulsed-FRAP (panel a). (rug.nl)

Inside the mitochondria, pyruvate is completely oxidized through the TCA cycle, feeding reductive equivalents to the electron transport chain. (hindawi.com)
Electron transport chain (ETC) and its mechanism. (amrita.edu)

Also known as the breast cancer resistance protein (BCRP), ABCG2 has physiological roles in various tissue cells including the mammary gland and the blood-brain , blood-testis , and maternal-fetal barriers. (proteopedia.org)
Substrates are transported through ABCG2 via two cavities separated by a leucine plug (Figure 2). (proteopedia.org)

Heide, T. van der, Stuart, M.C.A., and Poolman, B. (2001) On the osmotic signal and osmosensing mechanism of an ABC transport system for glycine betaine. (rug.nl)
Though purine nucleotides (PN) inhibit UCP3-mediated transport, the molecular mechanism differs from that of UCP1 . (aabioetica.org)
They catalyze glucuronidation reactions in various aglycone substrates, contributing significantly to the body's chemical defense mechanism. (mdpi.com)

The oxidized flavin nucleotide can accept either one electron (or) two (yielding FADH 2 (or) FMNH 2 ). (biochemden.com)
The standard reduction potential of a flavin nucleotide, unlike that of NAD (or) NADP, depends on the protein with which it is associated. (biochemden.com)
The flavin nucleotide should be considered part of the flavoproteins active site, not as a resultant (or) product6 in the electron-transfer reaction. (biochemden.com)

Both of the integral membrane protein constituents of these systems may be distantly related, and in this respect they resemble typical ABC porters. (tcdb.org)

Kocer, A., Walko, M., and Feringa, B.L. (2007) Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL. (rug.nl)

The uncharacterized periplasmic components of various ABC-type transport systems are included in this group. (unl.edu)

BioMNY-mediated biotin uptake was severely impaired by replacement of the Walker A lysine residue of BioM, demonstrating dependency of high-affinity transport on a functional ATPase. (tcdb.org)

Fluorescent esterase substrates may also be used in cell viability assays in place of tetrazolium analogs such as MTT or WST. (dojindo.com)

Here we define the precise Rab29 binding region of the LRRK2 Armadillo domain between residues 360-450 and show that this domain, termed 'Site #1', can also bind additional LRRK2 substrates, Rab8A and Rab10. (stanford.edu)
These PBPs bind their substrates selectively and with high affinity. (unl.edu)

HEK293 cells had an efficient ef flux system for cGMP and the use of inside-out vesicles (IOVs) showed high affinity ATP-dependent cGMP transport with a K m value of 2.3 μM. (scirp.org)

Glucose and glutamine are the 2 major substrates used by cancer cells. (hindawi.com)

Heterologous expression of the bioMNY operon and of the single bioY of the α-proteobacterium Rhodobacter capsulatus conferred biotin-transport activity on recombinant E. coli cells. (tcdb.org)
The recombinant MRP1 protein expressed and trafficked normally to the plasma membrane. (sdstate.edu)
The HuProt microarray results showed that the signals of 343 proteins were higher in the recombinant CRYAA group than in the control group. (molvis.org)
This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)

To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
Cyt.c of mitochondria, a soluble protein that associates through electrostatic interactions with the outer surface of the inner membrane. (biochemden.com)

2023 demonstrated that BioY linked to BioB can transport not only biotin but also dethiobiotin, and the combination of BioY and BioB contributes to the growth of A. caulinodans ORS571 in a biotin-deficient but dethiobiotin-sufficient environment. (tcdb.org)

However, the VUT family member, TrpP of Bacillus subtilis (2.A.88.4.1) and the ThiW ABC membrane protein homologue, 3.A.1.26.2, are clearly related by common descent. (tcdb.org)

van den Bogaart, G., Krasnikov, V., and Poolman, B. (2006) Dual-color fluorescence burst analysis to probe protein diffusion through the mechanosensitive channel MscL. (rug.nl)
Fluorogenic esterase substrates that can be passively loaded into viable cells, such as Calcein-AM, BCECFAM, Carboxyfluorescein succinimidyl ester (CFSE), and Fluorescein diacetate (FDA), are converted by intracellular esterases into fluorescein analogs with green fluorescence. (dojindo.com)

Scope includes mutations and abnormal protein expression. (cancerindex.org)

A combination of molecular simulation with hydrogendeuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP-induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. (elifesciences.org)
Molecular dynamics (MD) simulations highlight the improved stability for the substrate in the active site when ions are present, suggesting a binding order during the transport cycle. (elifesciences.org)

Transcellular transport experiments have shown that BCRP transports sulfated estrogens and various sulfated steroidal compounds, but not free estrogens. (elsevierpure.com)
Physiological concentrations of estrogens (10-100 pM) reduced BCRP protein expression without affecting its mRNA levels. (elsevierpure.com)
The C376T (Q126Stop) polymorphism has a dramatic phenotype as active BCRP protein cannot be expressed from a C376T allele. (elsevierpure.com)
The C421A (Q141K) polymorphism is also significant as Q141K-BCRP-transfected cells show markedly low protein expression levels and low-level drug resistance. (elsevierpure.com)
Hence, individuals with C376T or C421A polymorphisms may express low levels of BCRP or none at all, resulting in hypersensitivity of normal cells to BCRP-substrate anticancer agents. (elsevierpure.com)

Multidrug resistance protein 1 (MRP1) actively transports a wide variety of drugs out of cells. (sdstate.edu)

The protein is in the inward-facing conformation with Cavity 1 open to the cytosol for substrate recruitment, the Leucine Plug intact, and Cavity 2 completely occluded. (proteopedia.org)

This collapse forces the substrate to move forward to Cavity 2 as there is no longer room in Cavity 1 to accommodate substrates. (proteopedia.org)
Cavity 2 contains a less hydrophobic environment and, as a result, substrates are released due to hydrophobic mismatch. (proteopedia.org)

For instance, analysis of proteins in the manner of displaying their structures. (projectguru.in)
Dr. Berger's team has solved structures of topoisomerase targets in complex with target DNA substrates and various drugs. (hopkinsmedicine.org)

One-hundred and twenty-seven of 17,225 human full-length proteins were identified that interact with CRYAA. (molvis.org)

We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
Despite the proposed role for nerve growth factor (NGF) in this sprouting, we observed no change in NGF mRNA or protein at several postlesion time points. (jneurosci.org)

Thus, MSA is useful in bioremediation studies to understand the protein lineage of an important enzyme or protein. (projectguru.in)
However, the mystery of patients with these clinical symptoms (despite normal phosphatase activity) remained unsolved until 1978, when Narisawa et al identified a defect in intracellular transport of the enzyme substrate. (medscape.com)
glycogen-storage disease type Ia (GSD Ia) designates the true enzyme defect, and glycogen-storage disease type Ib (GSD Ib) designates the intracellular transport defect. (medscape.com)

The capacity of mouse CABYR isoforms to associate into dimers and oligomers, and the relationships between CABYR and other FS proteins were studied by gel electrophoresis, Western blotting, immunofluorescence, immunoprecipitation and yeast two-hybrid analyses. (biomedcentral.com)

The ATP/ADP uniporters can also transport inorganic phosphate, but not ribonucleoside and monophosphates, as well as deoxyribonucleotides. (wikipedia.org)
2014). It also reduces Hg 2+ ions using Nicotinamide adenine dinucleotide phosphate (NADPH) as a substrate. (projectguru.in)

We have proposed that under conditions of severe osmotic stress (panel b, lower figure), the cell membrane contacts the nucleoid, which creates pools of proteins and hinders free diffusion through the cell. (rug.nl)
Nucleotide staining with fluorescent intercalators is mostly used for dead cell detection. (dojindo.com)
These esterase substrates, therefore, can serve as assay probes of cell viability. (dojindo.com)
Tripartite Motif Containing 13 (TRIM13), a member of TRIM proteins, is deleted in multiple tumor types, especially in B-cell chronic lymphocytic leukemia and multiple myeloma. (cancerindex.org)
Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
5 This is about 1/8 the size (therefore information content) of a typical protein, yet the hypothetical simple cell above needs at least 256 proteins. (creation.com)
Consequently, it is implausible that a completely "denatured" cell could be reversibly renatured spontaneously, like a protein. (creation.com)
The proteins of such MAPKs increased and were then repress UniProt differential download Sarkozy, Israël et whereby steps include to the spliced group before any further cell. (erik-mill.de)
202:849) controlling cell-substrate adhesion. (ucsf.edu)
The high impact our pioneering work bridging protein electrostatics and cell biology is highlighted by our publication h-index of 46. (ucsf.edu)

MSA refers to the alignment of three or more biological sequences, protein or nucleic acid of similar length. (projectguru.in)

showing the cavities through the channel, with the protein in grey surface, the pre-protein pore constrictions in red (SecY) or purple (SecA) mesh, and the SecY plug in red helix. (elifesciences.org)

antibody responses to polysaccharide antigens are decreased and antibody responses to protein antigens are slightly reduced. (medscape.com)
Immunohistochemistry utilizing anti- UCP1 antibody , in addition to Western blot evaluation verified UCP1 protein expression within the wild-type adrenal medulla. (aabioetica.org)

The ESCRT portion tends typically human for receptor-ligand transport that is fulfilment metabolized unidirectional binds. (erik-mill.de)

Anatomy8

Organisms4

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes30

Information Science4

Health Care1

Specificity6

Guanine Nucleot1

Transmembrane5

Cyclic Nucleotides1

Amino14

Glycolysis4

Enzymes7

Phosphorylation3

Transporters13

Lipids1

Transporter10

Nucleic acids1

Membranes9

Gene13

Fatty acids1

Kinase1

Covalently2

Biosynthesis1

Extracellular2

Sequence8

Nucleoside3

Solute2

Superfamily2

Mechanisms3

Secretion4

Mitochondrial2

Genes1

Homologous2

Chromosome1

Complexes2

Target proteins1

Bacterial1

Electron trans2

ABCG22

Mechanism3

Flavin3

Distantly related1

Synthesis1

Periplasmic1

Uptake1

Assays1

Bind2

High-affinity1

Glucose1

Recombinant4

Interactions5

20231

Membrane protein1

Fluorescence2

Mutations1

Molecular2

BCRP5

Multidrug1

Cytosol1

Cavity2

Structures2

Interact1

MRNA2

Enzyme3

Analyses1

Phosphate2

Cell10

Biological1

Cavities1

Antibody2

Ligand1