Transport of rna. Medical search. Frequent questions

This manuscript reports data consistent with a new and unanticipated phenomenon: that Cre or its mRNA may be transmitted between tissues in the mouse and that the male reproductive tract (epididymis) appears to be the most common target of such transported molecules. (elifesciences.org)
RNA-targeting small molecules represent a class of small molecules, organic compounds with traditional drug properties (e.g. (wikipedia.org)
For these reasons, RNA is being recognized as an attractive drug target for small molecules. (wikipedia.org)
David Wilson and David Draper were the first to suggest that RNA structures could be targeted by small molecules. (wikipedia.org)
Small molecules may present a better way to target RNA and subsequently DNA because they can be designed to be more "drug-like" and have a better chance of reaching their target, most by oral administration. (wikipedia.org)
For this reason, there is an emerging interest in designing and discovering small molecules to target RNA secondary and tertiary structures to ultimately treat new diseases. (wikipedia.org)
citation needed] There are limited examples of small molecules that target RNA and are approved drugs for the treatment of human disease. (wikipedia.org)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
Transfer RNA (tRNA) molecules contain various post-transcriptional modifications that are crucial for tRNA stability, translation efficiency, and fidelity. (mdpi.com)
Within cells, citrin helps transport molecules used in the production and breakdown of simple sugars, the production of proteins, and the urea cycle. (medlineplus.gov)
Using the yeast three hybrid assay, we selected RNA molecules that retain Tap binding activity from a pool of randomized CTE sequences. (princeton.edu)
By using small interfering RNA (siRNA) molecules targeted to endogenous alpha-synuclein, we achieved 80% protein knockdown. (ox.ac.uk)
All RNA molecules longer than 18 nucleotides are purified. (qiagen.com)
The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules. (microbiologyresearch.org)
At least in plants, DNA regions that become de novo methylated can be defined by homologous RNA molecules in a process termed RNA-directed DNA methylation (RdDM). (degruyter.com)
Viroids are pathogenic circular, non-coding, single-stranded RNA molecules. (degruyter.com)
Viroids are non-encapsidated, single-stranded (ss), 250-400 nucleotide (nt)-long circular RNA molecules that do not encode proteins (1, 2). (degruyter.com)
Upon inoculation, mature Pospiviroidae RNA molecules enter the plant cell nucleus. (degruyter.com)
The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)

Context and state of the art mRNA transport in general: Cytoplasmic mRNA transport and local translation are essential for many processes requiring symmetry breaking such as embryonic development, cell migration and neuronal differentiation (1). (europa.eu)
RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
Since the first discovery of cytoplasmic mRNA transport almost three decades ago, several essential questions could not be answered mostly due to the complexity of the in vivo situation and the approaches available. (europa.eu)
mRNA transport in neurons: In neurons, thousands of mRNAs are transported into axons or dendrites by so far not identified transport mechanisms (6). (europa.eu)
Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)
Neuronal mRNA transport occurs in packages of single to a few copies of mRNAs (8-11), mostly in a translationally repressed mode. (europa.eu)
After induction of long-term potentiation, CaMKIIa-mRNA is transported to distal parts of dendrites where its translation is locally regulated (12, 13). (europa.eu)
Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)
Before we can understand how overall distributions of thousands of mRNA are generated, we first need to understand how any specific mRNA can be transported along microtubules - a question unanswered since the first MT based mRNA transport was observed more than 2 decades ago. (europa.eu)
Es gibt drei RNA-Typen, die in der Proteinsynthese eine Rolle spielen: Boten-RNA (englisch messenger RNA, mRNA), Transfer-RNA (tRNA) und die ribosomale RNA (rRNA). (jove.com)
Die Boten-RNA (mRNA) ist eine proteinkodierende RNA, die aus Codons besteht. (jove.com)
As a nucleoside inhibitor, the guanosine analog prodrug is used to stop viral RNA synthesis and viral mRNA capping by incorporating into RNA and pairing to uracil or cytosine. (wikipedia.org)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
In Caenorhabditis elegans , RNA-dependent RNA polymerases (RdRPs) are used for the small RNA-guided production of additional small RNAs 8 that are complementary to terminally modified mRNA fragments 9 . (nature.com)
However, the mere presence of small RNAs (Supplementary Table 1 ) or terminally modified mRNA fragments 9 does not result in indefinite RNA silencing. (nature.com)
RNA binding proteins can control protein expression by regulating transport, localization, splicing, polyadenylation and turnover of mRNA transcripts. (umassmed.edu)
Decreased expression of the RNA-binding protein, Pumilio, stabilizes predicted neural mRNA targets and presence of a 3'UTR PRE is sufficient to trigger mRNA decay in the nervous system. (escholarship.org)
The human Tap protein has been proposed to mediate Mason Pfizer monkey virus constitutive transport element (CTE)-dependent nuclear RNA export and may also play a role in global mRNA export. (princeton.edu)
Cytoplasmic dynein-1 (dynein) is an essential motor complex for mRNA transport and localisation. (biologists.com)
These studies provide a new paradigm of how mRNA and probably other cargos could stimulate their own transport. (biologists.com)
To expand understanding of the role of snoRNAs in control of gene expression, in this study we tested the ability of artificial box C/D RNAs to affect the maturation of target pre-mRNA. (hindawi.com)
The artificial box C/D RNA directed to the branch point adenosine of the second intron, as well as the analogue directed to the last nucleotide of the second exon of the HSPA8 pre-mRNA caused the most prominent influence on the level of HSPA8 mRNAs. (hindawi.com)
Inasmuch as artificial box C/D RNAs decreased viability of transfected human cells, we propose that natural snoRNAs as well as their artificial analogues can influence the maturation of complementary pre-mRNA and can be effective regulators of vital cellular processes. (hindawi.com)
Using such an approach, it was shown that introducing sequences complementary to a selected pre-mRNA into box C/D RNA structures allowed knockdown of the target mRNA [ 13 ]. (hindawi.com)
One of the two main steps of gene expression is the translation of messenger RNA (mRNA) into polypeptide sequences of amino acids. (lirmm.fr)

Green and coworkers further confirmed this idea, discovering that aminoglycosides blocked the interaction of HIV-1 Rev protein and its viral RNA-binding site. (wikipedia.org)
The QIAprep& Viral RNA UM Kit and QIAprep& Viral RNA QIAsymphony Kit are intended for molecular biology applications. (qiagen.com)
The QIAprep& Viral RNA UM Kit is an innovative liquid-based approach to viral RNA epidemiology. (qiagen.com)
The QIAprep& Viral RNA QIAsymphony Kit can be used for automated sample preparation and assay set up on the QIAsymphony SP/AS. (qiagen.com)
The QIAprep& Viral RNA QIAsymphony Kit requires the installation of a custom protocol available from QIAGEN Services. (qiagen.com)
The QIAprep& Viral RNA UM Kit can be used in conjunction with the SARS-CoV-2 N1+N2 Assay Kit . (qiagen.com)
Want to try the QIAprep& Viral RNA UM Kit for the first time? (qiagen.com)
The assay performance in terms of specificity and sensitivity has been verified using the QIAprep& Viral RNA UM Kit. (qiagen.com)
The end-to-end liquid-based workflow of the QIAprep& Viral RNA UM Kit takes under one hour from start to finish. (qiagen.com)
We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
In addition, detection of viral RNA by reverse transcription--polymerase chain reaction (RT-PCR), usually in a throat swab or urine sample, and subsequent genotyping of strains is valuable for diagnosis and molecular epidemiology ( 2 ). (cdc.gov)
Accurate laboratory results for detection of IgM and viral RNA are dependent on proper collection, processing, shipment, and storage of clinical samples and use of accurate tests performed by a proficient laboratory. (cdc.gov)
DBS has been used for various epidemiologic studies for the detection of measles- and rubella-specific IgG and IgM antibodies and viral RNA ( 3--5 ). (cdc.gov)
Labelling with BrUTP showed that peroxisomes were the sites of nascent viral synthesis, whereas in situ hybridization indicated that DI RNA progeny were diffused throughout the cytoplasm. (microbiologyresearch.org)
SARS-CoV in 1,048 cases investigated for SARS in the were investigated for SARS coronavirus (SARS-CoV) first 5 weeks after the first-generation diagnostic tests through RNA tests, serologic response, and viral culture. (cdc.gov)
5 days of illness, but later in the illness viral RNA could be detected more readily in stool specimens. (cdc.gov)
Patients were categorized on a clinical basis as ratory tests to detect viral RNA and antibody responses (5) "clinical SARS," "suspected SARS," and "not SARS" by were used to establish a cause in patients suspected to have the attending clinicians, depending on the response to SARS. (cdc.gov)
Throat-swab specimens in viral transport media were transported to the national reference laboratory within 72 h of collection using a cold-chain system. (cdc.gov)
We extracted viral RNA from throat-swabs and subjected to real-time PCR amplification. (cdc.gov)

It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
Through mechanisms that remain poorly defined, defects in nucleocytoplasmic transport and accumulations of specific nuclear-pore-complex-associated proteins have been reported in multiple neurodegenerative diseases, including C9orf72 Amyotrophic Lateral Sclerosis and Frontotemporal Dementia (ALS/FTD). (nih.gov)
Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure RNA to be eluted in either water or a buffer provided with the kit. (qiagen.com)
Characterization of the structure and dynamics of protein-RNA complexes is essential for understanding how they function at the molecular level: how these proteins specifically recognize their target RNAs, what are the structure-function relationships, and assess their biological role in regulating gene expression. (umassmed.edu)
Characterization of the structure and dynamics of protein-RNA complexes is essential for understanding how they function at the molecular level: how these proteins specifically recognize their target RNAs, what are the structure-function relationships, and to assess their biological role in regulating gene expression. (umassmed.edu)
Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (exosome-rna.com)
This observation fits very nicely with the recent structural studies showing that adaptor proteins make transport faster by recruiting two dyneins at the cargo 3,4 . (biologists.com)
The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
Box C/D RNAs are associated with four snoRNP core proteins: NOP56, NOP58, fibrillarin, and 15.5 kDa [ 5 - 7 ]. (hindawi.com)
Box C/D RNAs contain the following functional elements: boxes C and D, which are essential for snoRNA interaction with specific proteins, and a guide sequence that determines the nucleotide to be modified (Figure 1(a) ). (hindawi.com)
His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)

Howev- K9 remained negative, whereas K8 was positive for er, the pathophysiology and epidemiologic impact of SARS-CoV-2 RNA until day 21 of surveillance. (cdc.gov)

Here we report that a minimal combination of cis- regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. (nature.com)
This stable silencing requires components of the small RNA pathway and can silence homologous sequences in trans . (nature.com)
The recovered RNA sequences differed only minimally from the wild-type CTE yet all displayed lower affinity for Tap both in vivo and in vitro. (princeton.edu)
The RNAi-microsponges developed by a multidisciplinary team at the Massachusetts Institute of Technology (MIT) are constructs of long RNA polymers comprising repeating sequences of the designated siRNA. (genengnews.com)
The circle was closed using T4 DNA ligase, and the circular DNA then used to produce RNA transcripts by RCT, which encode both antisense and sense sequences of the anti-luciferase siRNA. (genengnews.com)
The recruitment of this machinery results in the production of viroid-derived small RNAs (vd-sRNAs) that mediate RNA degradation and DNA methylation of cognate sequences. (degruyter.com)

In an early report, Noller discovered that several classes of antibiotics (streptomycin, tetracycline, spectinomycin, edeine, hygromycin, and the neomycins) could "protect" nucleotides in 16S ribosomal RNA by binding to this RNA. (wikipedia.org)
Small nucleolar RNAs (snoRNA) are commonly known to be involved in the processing of precursor ribosomal RNA (pre-rRNA) and small nuclear RNAs (snRNAs). (hindawi.com)

By this principle, if one can identify an RNA involved in disease then the sequence can be used to design a complementary antisense oligonucleotide, and that agent can be introduced into cells to treat the disease. (wikipedia.org)
Transport of 16 substrates was measured for each individual human OATP in complementary RNA-injected Xenopus laevis oocytes. (nih.gov)
Bioinformatics analysis revealed that some of these orphan RNAs were complementary to pre-mRNAs or mature mRNAs [ 10 ]. (hindawi.com)

To investigate this, we have developed a system in which levels of alpha-synuclein can be acutely suppressed by using RNA interference (RNAi) in a physiologically relevant human dopaminergic cellular model. (ox.ac.uk)
Because the RNAi-microsponges are essentially highly concentrated balls of nearly 100% interfering RNA (RNAi), they should be an effective means of delivering siRNA to target cells. (genengnews.com)
Since then, he has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and his main field of interest is RNA-interference (RNAi) and RNA-directed DNA methylation (RdDM) in plants. (degruyter.com)
Since then, she has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and her main field of interest is RNA-interference (RNAi) and viroid biology in plants. (degruyter.com)

As another strategy for targeting RNA, antisense oligonucleotides were developed which have been pushed forward through the clinic for several diseases. (wikipedia.org)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
In study by Ono and coworkers, such an antisense element was placed into HBII-180C box C/D RNA instead of the structural element referred to as the M box [ 13 ]. (hindawi.com)

Since 2001, LabNet reference laboratories in Australia, Cote d'Ivoire, Netherlands, Turkey, Uganda, the UK, and the United States have been working to 1) determine IgM and RNA stability in DBS and OF samples and 2) optimize the methods for IgM antibody assay and protocols for RNA detection in DBS and OF samples ( 8--10 ). (cdc.gov)

Total RNA binds to the QIAamp membrane and contaminants are washed away, leaving pure RNA to be eluted in 30-100 µl RNase-free water (provided with the kit) for direct use in any downstream application. (qiagen.com)
The PAXgene Tissue miRNA Kit provides purification of total RNA, including RNA from approximately 18 nucleotides, from tissues fixed and stabilized in PAXgene Tissue Containers. (qiagen.com)
Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality miRNA and total RNA for molecular analysis. (qiagen.com)
Total RNA purified using the PAXgene Tissue miRNA Kit is highly pure. (qiagen.com)
The system consists of a tissue collection device (the PAXgene Tissue Container for collection, stabilization, storage, and transportation of human tissue specimens) and kits for purification of miRNA, total RNA, or DNA. (qiagen.com)
For purification of total RNA including miRNA, the system requires the use of PAXgene Tissue Containers for tissue collection and stabilization, followed by RNA isolation and purification using the PAXgene Tissue miRNA Kit. (qiagen.com)

More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)
For example, although the presence of RNA amplification correlates with reported cases of persistent RNA silencing, most induced silencing dissipates within a few generations (reviewed in ref. 7 ). (nature.com)

Lipinski's rule of five) that can bind to RNA secondary or tertiary structures and alter translation patterns, localization, and degradation. (wikipedia.org)
Investigators develop degradation-resistant microspheres composed of RNA as both carrier and cargo. (genengnews.com)
These RNA strands self assemble into incredibly dense crystalline microspheres, which resist degradation, are efficiently taken up into cells, and then are simply chopped up by the cell's own enzymes into vast numbers of active siRNAs. (genengnews.com)
The transport solutions in these tubes release the rRNA targets and protect them from degradation during storage. (cdc.gov)

Schroeder uncovered that aminoglycosides could inhibit protein synthesis by interacting with the ribosome through interactions with the 3' end of the 16S RNA of E. coli taking advantage of RNA conformational changes. (wikipedia.org)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)

Whereas 85% of the human genome is transcribed into RNA only 3% of the transcripts code for functional protein. (wikipedia.org)

However, measles and rubella surveillance remains incomplete in certain areas because of difficulties with the collection and transport of serum specimens. (cdc.gov)
Specimens are collected and transferred into their respective specimen transport tubes. (cdc.gov)

Collectively, our data suggest that the expression of expanded C9orf72 ALS/FTD repeat RNA alone affects nuclear POM121 expression in the initiation of a pathological cascade affecting nucleoporin levels within neuronal nuclei and ultimately downstream neuronal survival. (nih.gov)
Genomic DNA contamination is minimized, and purified RNA is ready to use in downstream applications with no detectable PCR inhibition. (qiagen.com)
In this JoVE video, we will be sharing with you information about the collection and processing of lymph nodes from large animals for use in downstream RNA experiments. (jove.com)
Completely inactivates harmful pathogens at the point of collection, for worry-free transport and downstream analysis. (zymoresearch.com)

With the help of a new single molecule RNA transport assay from the purified components (McClintock et al. (biologists.com)
In the transport assay, authors were able to achieve only half of the normal dynein speed. (biologists.com)

RNA interference-mediated knockdown of alpha-synuclein protects human dopaminergic neuroblastoma cells from MPP(+) toxicity and reduces dopamine transport. (ox.ac.uk)
They report their achievement in Nature Materials , in a paper titled "Self-assembled RNA interference microsponges for efficient siRNA delivery. (genengnews.com)

While it is clear, that RNA distribution patterns in neurons are created by active transport processes of mRNPs along microtubules, the essential enzymatic activities required are not known. (europa.eu)
RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)

Those authors did not include rRNA or tRNA genes, and they recognized that the basic substrate transport machinery could not be clearly defined, even though this minimal cell would rely greatly on the import of several substrates, including all 20 amino acids (for which it had no biosynthetic ability). (creation.com)

87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)

Members of the Pospiviroidae family replicate in the nucleus through double-stranded RNA intermediates, attracting the host RNA silencing machinery. (degruyter.com)

The ssRNA(+), likely transferred into the nucleolus, is finally processed by a type III RNase and a DNA LIGASE into unit-length circular RNAs (8) ( Figure 1 ). (degruyter.com)

b) Base-pairing interactions between an artificial box C/D RNA and target RNAs. (hindawi.com)

Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory functions ranging from translation regulation to gene expression control and cellular stress response. (mdpi.com)

This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA analysis. (jove.com)

The expression levels of genes involved in neuronal and oligodendrocyte maturation, and angiogenic and transport functions were al-tered, reflecting enhanced brain maturation in response to IGF-1 treatment. (lu.se)

In order for cellular RNA to be effective it must enter the cells intact. (wikipedia.org)
The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. (qiagen.com)
The QIAamp RNA Blood Mini Kit provides purification of cellular RNA using silica-membrane technology. (qiagen.com)
QIAamp technology yields total cellular RNA from fresh whole blood and other sample sources that is ready to use in RT-PCR and blotting procedures. (qiagen.com)
QIAamp RNA Blood Mini Kits provide a fast, easy method for the preparation of total cellular RNA from up to 1.5 ml of human whole blood. (qiagen.com)
Lymph nodes are useful samples for the assessment of host immune responses but can also provide a source of pathogen RNA for study, such as the case of intra-cellular pathogens present in immune cells inside lymph nodes. (jove.com)

Here, we have used in vivo assays, in both yeast and human cells, together with in vitro assays, to further characterize the RNA binding properties of Tap, which has been proposed to contain a novel leucine-rich RNA binding motif. (princeton.edu)

mRNAs are transported along microtubules (MTs) by different kinesins and cytoplasmic dynein and get anchored at the actin cortex, intermediate filaments or unknown structures (1, 3). (europa.eu)
This defines the extent to which transport of an mRNP is biased towards one direction, which ultimately gives rise to the steady state distributions pattern of mRNAs observed (5). (europa.eu)

In these cases, the QIAGEN RNase-Free DNase Set provides convenient on-column DNase treatment of RNA samples during QIAamp RNA procedures. (qiagen.com)

Hepatic uptake of cholephilic organic compounds is mediated by members of the organic anion-transporting polypeptide (OATP) family. (nih.gov)

The kit is optimized for ultrafast sample preparation and sensitive real-time PCR detection of enveloped RNA viruses such as coronaviruses from human samples (nasal, oro- and nasopharyngeal swabs) collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. (qiagen.com)
During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)

Here, we'll present a protocol for the isolation of large animal lymph nodes for RNA study that will be informative for molecular biologists involved in the transcriptomic studies of large animal infections, as well as veterinary researchers commencing transcriptomic studies. (jove.com)

The QIAamp RNA Blood Mini Kit is intended for molecular biology applications. (qiagen.com)

The SafeCollect devices are filled with DNA/RNA Shield, which is compatible with most commercially available DNA/RNA extraction/analysis workflows. (zymoresearch.com)

RNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. (qiagen.com)

In 2006, "follow-up research led by Hamilton Smith at the J. Craig Venter Institute in Rockville reveals that the minimum genome consists of 387 protein-coding and 43 RNA-coding genes. (creation.com)

cDNA was synthesized from 10 μ L of RNA eluate using random hexamer primers (TaKaRa Dalian, China) and SuperScript II Reverse Transcriptase (Invitrogen Carlsbad, Calif, USA). (hindawi.com)

and 3) DBS or OF sampling are viable options for measles and rubella surveillance in all regions, especially where patients might resist venipuncture for blood collection, or where special challenges exist with transport or refrigeration of diagnostic samples. (cdc.gov)
However, collection of blood samples by venipuncture, particularly from children, can be a challenge, and the sustained refrigeration required for diagnostic samples during transport is not always achievable. (cdc.gov)
Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of miRNA, RNA, and/or DNA. (qiagen.com)
The DNA/RNA Shield SafeCollect Saliva Collection Kit is a user-friendly saliva collection kit for stabilizing the nucleic acid content of saliva and sputum samples. (zymoresearch.com)
Pigs were treated with 2.25 mg/kg/d recombinant human IGF-1/IGF binding protein-3 complex from birth until day 5 or 9 before the collection of brain samples for quantitative immunohistochemistry (IHC), RNA sequencing, and quantitative PCR analyses. (lu.se)

RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
Critically, once internalized in cells, the RNA is split up into the 21 nucleotide-long RNA component units by activity of the enzyme Dicer, and then converted to active siRNAs by the RNA-induced silencing complex. (genengnews.com)

Further, RNA can adopt discrete secondary or tertiary structures which play a pivotal role in many biological processes and disease pathology. (wikipedia.org)
They hypothesized that RNA could be "druggable" by targeting the 3D structure in the same way as protein 3D structures are used as drug targets and furthered the idea that targeting RNA could be used to treat diseases. (wikipedia.org)
By 12 hours the sheet structures began to develop wrinkled and semi-spherical constructs, and after 16 hours the RNA polymer continued to morph into interconnected globular superstructures. (genengnews.com)

Phosphate is the most abundant intracellular anion and is essential for membrane structure, energy storage, and transport in all cells. (medscape.com)

For their proof of principle work the team designed the RNA polymer to encode repeating units of an anti-luciferase siRNA, which they could then test in experimental models. (genengnews.com)

Researchers at Stanford University used such a method to produce amplified heterogeneous populations of RNA from limited quantities of cDNA. (genomicglossaries.com)
Amplified RNA synthesized from limited quantities of heterogeneous cDNA. (genomicglossaries.com)

rRNAs and snRNAs are the main natural targets of 2′-O-methylation that is mediated by box C/D RNAs. (hindawi.com)

Short interfering RNAs (siRNAs) represent a promising therapeutic option for gene silencing, but one of the major hurdles to their clinical success is the difficulty in designing an efficient delivery method. (genengnews.com)

Using the zeta (ζ)-potential as a surrogate for surface charge, researchers from Tel Aviv University show that the deposition of EVs from the yeast, Saccharomyces cerevisiae , and two bacterial species, Staphylococcus aureus and Pseudomonas fluorescens , in well-characterized porous medium under various background conditions influence the transport of other environmental colloidal particles: ionic strength and humic acid concentration. (exosome-rna.com)
Rogers NMK, Hicks E, Kan C, Martin E, Gao L, Limso C, Hendren CO, Kuehn M, Wiesner MR. (2023) Characterizing the Transport and Surface Affinity of Extracellular Vesicles Isolated from Yeast and Bacteria in Well-Characterized Porous Media . (exosome-rna.com)
Two yeast strains were used, differing in the biogenesis of peroxisomes, the organelles supplying the membranous vesicular environment in which CymRSV RNA replication takes place in infected plant cells. (microbiologyresearch.org)
DI RNA replication occurred in yeast cells, as demonstrated by the presence of monomers and dimers of positive and negative polarities. (microbiologyresearch.org)
DI RNA replication also took place in yeast cells devoid of peroxisomes. (microbiologyresearch.org)

For example, these experiments might include quantitative PCR on reverse transcribed products, or RNA sequencing studies of transcriptomes. (jove.com)

With this concept as their goal, the MIT researchers applied an enzymatic RNA polymerization technique, known as rolling circle transcription (RCT), to generate elongated polymers of pure RNA that could self assemble into organized nano- to microstructures. (genengnews.com)

The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)

In addition, significant increases in interleukin-4 messenger RNA and protein expression in the DLNs were observed in OPA-treated groups. (cdc.gov)

After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. (qiagen.com)
Purification is carried out using silica-based RNA purification technology in a spin-column format. (qiagen.com)

En vous déconnectant d'EU Login, vous vous déconnecterez de tous les autres services utilisant votre compte EU Login. (europa.eu)

Together, our findings should motivate caution when interpreting conditional alleles, and suggest the exciting possibility of inter-tissue RNA or protein trafficking in modulation of reproductive biology. (elifesciences.org)

Analysis of the RNA export activity of the recovered CTE variants revealed that Tap affinity was highly predictive of CTE biological activity. (princeton.edu)

It will be interesting to see whether other types of dynein cargos (e.g. vesicles) could influence transport in a similar way. (biologists.com)

This effectively generates nanoscale pleated sheets of hairpin RNA that self-assemble into sponge-like microspheres. (genengnews.com)

This work combined with advances in structural characterization techniques such as NMR spectroscopy and X-ray crystallography together with computational modeling, has pushed forward the realization that RNA is a dynamic yet viable drug target. (wikipedia.org)

Anatomy28

Organisms12

Diseases1

Chemicals and Drugs133

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes64

Technology, Industry, Agriculture1

Information Science3

Health Care1

Molecules21

MRNA26

Viral19

Proteins13

SARS-CoV-2 RNA1

Sequences6

Ribosomal2

Complementary3

RNAi4

Antisense4

Stability1

Total RNA6

Amplification2

Degradation4

Synthesis2

Transcripts1

Specimens2

Downstream4

Assay2

Interference2

Neurons2

RRNA1

Polymerase1

Intermediates1

Ligase1

Interactions1

Gene expression1

Tissues1

Maturation1

Cellular6

Vitro1

MRNAs2

DNase1

Polypeptide1

Detection2

Isolation1

Molecular1

Extraction1

Binds1

Genes1

Eluate1

Samples5

Enzyme2

Structures3

Membrane1

SiRNA1

CDNA2

Methylation1

Therapeutic1

Yeast5

Quantitative1

Enzymatic1

Stable1

Protein expression1

Spin column2

Login1

Biology1

Analysis1

Vesicles1

Generates1

Characterization1