Transplantation of syngeneic islets. Medical search. Frequent questions

METHODS: Th0, Th1, and Th2 cell lines specific for the class II MHC molecule H2IAb were adoptively transferred into T cell deficient, syngeneic, diabetic mice before transplantation of fully allogeneic C57BL/10 (H2b) or (CBKxBALB/c)F1 (H2k/d+Kb) islet grafts. (ox.ac.uk)
The qualifier value denotes the type of transplantation whether allogeneic, syngeneic, or zooplastic. (ahima.org)
Among 1462 patients who had allogeneic hematopoietic cell transplantation (HCT) between January 2000 and December 2005, 116 (7.9%) developed stage 3-4 gut GVHD. (nature.com)
Reduced mortality after allogeneic hematopoietic-cell transplantation. (nature.com)
When applied to stem cell transplantation anti-CD154 mAb treatment in combination with sublethal conditioning enables the generation of allogeneic hemopoietic chimerism and long term transplantation tolerance (11-14). (palomid529.com)
Nonobese diabetic or BALB/c mice were injected i.p. with xenogeneic, allogeneic, or syngeneic cells, and the responding cells in subsequent lavages were assessed by flow cytometry and adoptive transfer. (aai.org)
Gastrointestinal acute graft-versus-host disease (GI aGVHD) is a lethal complication following allogeneic hematopoietic stem cell transplantation (HSCT). (biomedcentral.com)
We found that five proteins were increased at least 2-fold in the allogeneic group at day 7 compared with days 0, 3 and 15 (after Cyclosporin A treatment) and the syngeneic group at day 7. (biomedcentral.com)
Human hypoimmune primary pancreatic islets avoid rejection and autoimmunity and alleviate diabetes in allogeneic humanized mice. (ucsf.edu)

9. Hellerstr m C, Hellman B & T ljedal I-B: Quantitative studies on phosphatases in the pancreatic islet -cells. (taljedal.se)
Note that bone marrow, stem cell, and pancreatic islet cell transplants are not included in the Transplantation root operation. (ahima.org)

Kidney Transplantation Kidney transplantation is the most common type of solid organ transplantation. (merckmanuals.com)
The primary indication for kidney transplantation is End-stage renal failure. (merckmanuals.com)
Simultaneous islet cell-kidney transplantation may be desirable in the future after the outcomes have improved. (merckmanuals.com)
Streptozotocin-induced diabetes in rats was completely reversed by transplantation of syngeneic fetal pancreases placed beneath the kidney capsule. (diabetesjournals.org)
subterm Kidney, NEC identifies code 55.69, Other kidney transplantation. (ahima.org)
In monkeys it prolongs survival of islet (8) pores and skin (9) and kidney (10) allografts. (palomid529.com)

METHODS: Hepatocytes and ADSCs were cotransplanted into the renal subcapsular space and livers of syngeneic analbuminemic rats, and the serum albumin level was quantified to evaluate engraftment. (bvsalud.org)
12. T ljedal I-B, Hellman B, Petersson B & Hellerstr m C: Effect of glucagon on the alkaline phosphatase activity in the pancreatic islets of rats. (taljedal.se)
Materials and Methods: We evaluated respiratory activity using scanning electrochemical microscopy (SECM), glucose-stimulated respiratory activity, glucose-stimulated insulin release, ADP/ATP assays, insulin/DNA levels, and Trypan blue exclusion tests as predictive methods for the ability of isolated rat islets to cure syngeneic diabetic rats. (elsevierpure.com)
Results: Although glucose-stimulated respiratory activity, basal respiratory activity, ADP/ATP ratio, and glucose-stimulated insulin release were significantly correlated with the outcome of transplantation into diabetic rats, there was no correlation between outcomes, insulin/DNA ratios, and Trypan blue exclusion tests. (elsevierpure.com)
The glucose-stimulated respiratory activity in islet preparations that could cure diabetic rats was significantly greater than those unable to cure diabetes. (elsevierpure.com)
Methods and results: After coronary artery ligation in rats, the same numbers of syngeneic myoblasts were transplanted by intramyocardial injection or cell-sheet placement. (elsevierpure.com)
In rats it prolongs survival of islet allografts in autoimmune diabetic recipients L-Thyroxine (7). (palomid529.com)
FDG-labeled syngeneic islets or FDG alone were injected in rats. (unige.ch)

Introduction: The current methods for evaluating islet potency are not useful in clinical transplantation. (elsevierpure.com)

The interaction between primate blood and mouse islets induces accelerated clotting with islet destruction. (ox.ac.uk)
BACKGROUND: Mouse islets transplanted under the renal subcapsular space of cynomolgus monkeys are subject to a form of hyperacute rejection, the mechanism of which is unclear. (ox.ac.uk)
METHODS: Coagulation was assessed using thromboelastography on citrated/recalcified human blood samples with freshly isolated C57/Bl6 mouse islets. (ox.ac.uk)
RESULTS: Mouse islets incubated in human blood induced accelerated coagulation and rapid consumption of platelets within 15 min. (ox.ac.uk)
Within 1 h of incubation, 52% of mouse islets exposed to xenogeneic human blood showed features of severe damage with necrosis when compared with islets incubated in syngeneic blood. (ox.ac.uk)
CONCLUSION: Mouse islets induce rapid activation of the clotting cascade and platelet consumption in vitro when exposed to human blood, which correlated with histological evidence of significant destruction demonstrable within 1 h of exposure to human or non-human primate blood. (ox.ac.uk)
Intraportal transplantation of a marginal mass of syngeneic mouse islets was performed to evaluate the in vivo protective effect of WA. (elsevierpure.com)
Treatment of human and mouse islets with WA prevented cytokine-induced cell death, inhibited inflammatory cytokine secretion and protected islet potency. (elsevierpure.com)

To verify the utility of this method, we transplanted syngeneic rat islets subcutaneously into diabetic recipients under several oxygenation conditions using an oxygen delivery device, then performed pO2 measurement, glucose tolerance, and immunohistochemistry. (bvsalud.org)
Our recent study showed that retrievable and replaceable macro-encapsulated islets can be transplanted subcutaneously without pretreatment. (lidsen.com)

Dr. John Goss specializes in adult and pediatric liver transplantation, hepatobiliary surgery, and surgical management of liver tumors. (bcm.edu)
Geha JA, Geha JD, O'Mahony CA, Cotton RT, Galván TN, Rana A, Goss JA " Reconstruction of the Replaced Right Hepatic Artery Using Donor Iliac Arterial Y-Graft in Orthotopic Liver Transplantation . (bcm.edu)
Liver Transplantation. (bcm.edu)
Barshes NR, Udell IW, Joyce DL, Southard RE, O'Mahony CA, Goss JA " A pooled analysis of posttransplant survival following combined heart-liver transplantation. . (bcm.edu)
The liver site, currently utilized for transplantation, is suboptimal for achieving long-term insulin independence due to a rapid islet loss followed by a chronic decline in islet function after transplantation. (fujita-hu.ac.jp)
In vivo, all islets implanted in the liver, with an uptake 4.4 times higher than controls (44.2% vs. 10.1%, P=0.02). (unige.ch)
Radioactivity in the liver decreased at the same rate after injection of labeled-islets and FDG alone. (unige.ch)
Islets engrafted exclusively in the liver. (unige.ch)

eg, bone, bone marrow, and skin grafts) Genetically identical (syngeneic [between monozygotic twins]) donor tissue (isografts) Genetically. (merckmanuals.com)
T cells were 5-(and-6-)-carboxyfluorescein diacetate succinimidyl ester- (CFSE) labeled to allow detection, immunohistochemistry was performed, and IL-4 transcripts within the rejected islet grafts were quantified by reverse transcriptase polymerase chain reaction (RT-PCR). (ox.ac.uk)
CONCLUSIONS: Th2 and Th0 alloreactive CD4+ T helper cells can reject islet grafts with similar efficiency to Th1 cells. (ox.ac.uk)

Bone marrow transplants are the most common and well established cell transplantation therapies. (wikipedia.org)

Therefore, we evaluated the ability of WA to protect mouse and human islets from the damaging effects of pro-inflammatory cytokines in vitro and following intraportal transplantation. (elsevierpure.com)
Methods: Mouse and human islets were treated with a cytokine cocktail, and NF-κB activation was measured by immunoblots, p65 nuclear translocation and chromatin immunoprecipitation of p65-bound DNA. (elsevierpure.com)

Chimeras were normal CBA (H-2k) mice that were irradiated and given small numbers of syngeneic TCR-transgenic KB5 bone marrow cells. (palomid529.com)

A silicone spacer with GHNF was implanted into the subcutaneous space of healthy mice at 2, 4, 6, or 8 weeks before transplantation, and then diabetes was induced 7 days before transplantation. (bvsalud.org)
15. T ljedal I-B: Electrophoretic studies on phosphatases from the pancreatic islets of obese-hyperglycemic mice. (taljedal.se)
22. Haglund U & T ljedal I-B: Malate dehydrogenase isoenzymes in the pancreatic islets of obese-hyperglycemic mice. (taljedal.se)
Treatment of mice with a single donor-specific transfusion plus a brief course of anti-CD154 mAb uniformly induces donor-specific transplantation tolerance characterized by the deletion of alloreactive CD8+ T cells. (palomid529.com)
In mice it prolongs islet (3-5) and cardiac (6) allograft survival. (palomid529.com)
Combined therapy consisting of anti-CD154 mAb plus a L-Thyroxine solitary donor-specific transfusion (DST)5 in mice is definitely even more effective than anti-CD154 mAb monotherapy in prolonging the survival of islet pores and skin and heart allografts (5 6 15 The mechanism by which DST enhances graft survival appears in part to involve the deletion of recipient CD8+ alloreactive T cells (15). (palomid529.com)
0% for 200 islets + vehicle) in a mouse model of diabetes, compared with marginal graft controls with superior islet function in WA-treated mice confirmed by glucose tolerance test. (elsevierpure.com)

Islet transplantation offers a promising treatment for type 1 diabetes (T1D). (fujita-hu.ac.jp)
Intraportal islet transplantation (IT) is not widely utilized as a treatment for type 1 diabetes. (hindawi.com)
Aims/hypothesis: Beta cell death triggered by pro-inflammatory cytokines plays a central role in the pathogenesis of type 1 diabetes and loss of transplanted islets. (elsevierpure.com)

A secondary advantage is that islet cell transplantation appears to help maintain normoglycemia in patients who require total pancreatectomy for pain due to chronic pancreatitis. (merckmanuals.com)
Cell therapy (also called cellular therapy, cell transplantation, or cytotherapy) is a therapy in which viable cells are injected, grafted or implanted into a patient in order to effectuate a medicinal effect, for example, by transplanting T-cells capable of fighting cancer cells via cell-mediated immunity in the course of immunotherapy, or grafting stem cells to regenerate diseased tissues. (wikipedia.org)
In recent decades, however, stem cell and cell transplantation has gained significant interest by researchers as a potential new therapeutic strategy for a wide range of diseases, in particular for degenerative and immunogenic pathologies. (wikipedia.org)
For a long time, bone marrow transplantation was the only clinically applicable method of cell transplantation, however, since the 1990s, cell therapy has been investigated for a wide scale of pathologies and disorders. (wikipedia.org)
An acute graft-versus-host disease activity index to predict survival after hematopoietic cell transplantation with myeloablative conditioning regimens. (nature.com)
Prospective evaluation for upper gastrointestinal tract acute graft-versus-host disease after hematopoietic stem cell transplantation. (nature.com)

Taken together, the duration of GHNF pretreatment apparently had an impact on the outcomes of subcutaneous islet transplantation, and 6 weeks appeared to be the ideal duration. (bvsalud.org)

Wang S, Tasch J, Kheradmand T , Ulaszek J, Ely S, Zhang X, Hering BJ, Miller SD, Luo X. Transient B-cell depletion combined with apoptotic donor splenocytes induces xeno-specific T- and B-cell tolerance to islet xenografts. (uchicago.edu)

Conclusions: WA was shown to be a strong inhibitor of the inflammatory response in islets, protecting against cytokine-induced cell damage while improving survival of transplanted islets. (elsevierpure.com)

We previously revealed that a gelatin hydrogel nonwoven fabric (GHNF) markedly improved subcutaneous islet engraftment in comparison with intraportal islet transplantation. (bvsalud.org)
Subcutaneous tissue is a promising site for islet transplantation, but poor engraftment, due to hypoxia and low vascularity, hinders its prevalence. (bvsalud.org)
Interestingly, this novel technique revealed that islet viability estimated by ATP/DNA assay reduced to less than 75% by hypoxic condition at the SC, indicating that islet engraftment may substantially improve if the pO2 levels reach those of the renal subcapsular space. (bvsalud.org)
The adipose tissue-derived stem cells (ADSCs) are known to improve engraftment of transplanted pancreatic islets, which have many similarities to the hepatocytes. (bvsalud.org)

We herein investigated whether the duration of pretreatment using GHNF affected the outcome of subcutaneous islet transplantation. (bvsalud.org)
Islet graft revascularization, extracellular matrix compensation of the islet capsule, and the inflammatory status at the subcutaneous space would be crucial factors for successful subcutaneous islet transplantation. (bvsalud.org)
Further refinements for a hypoxic condition using the present technique may contribute to improving the efficiency of subcutaneous islet transplantation. (bvsalud.org)
As to the transplantation site, subcutaneous tissue can be promising, if new strategy can overcome the hypoxic status due to hypovascularity. (lidsen.com)
In this review article, we summarized the development of macro-encapsulated islets and approaches toward subcutaneous transplantation in our laboratory over more than two decades. (lidsen.com)
Our results repeatedly show that islets and encapsulated islets can be transplanted in various sites including subcutaneous tissue after pretreatment of neovascular induction. (lidsen.com)
Most recently, we found that slow release of hepatocyte growth factor (HGF) from the chitosan gel can protect islets against initial hypoxic status and subsequently induce neovascularization in subcutaneous tissue to exert islet function, eliminating the necessity for neovascularization pretreatment. (lidsen.com)

RESULTS: Adoptive transfer (IV) of Th0-, Th1-, and Th2 IAb-specific T cells resulted in rejection of H2b islet allografts. (ox.ac.uk)
Even when Th2 cells were introduced directly at the graft site, prompt rejection was still observed despite the presence of increased IL-4 mRNA expression within the islet allografts. (ox.ac.uk)

Oxygen levels remained constant at a level adequate to maintain islet viability in separate experiments. (ox.ac.uk)

Islet allograft rejection can be mediated by CD4+, alloantigen experienced, direct pathway T cells of TH1 and TH2 cytokine phenotype. (ox.ac.uk)
Nonvascularized xenografts, such as tissue cultured fetal pig pancreas (FPP) 3 or islets, are not prone to hyperacute rejection by preformed Abs, but are rejected within 10 days by an aggressive cell-mediated response that is difficult to prevent compared with allograft rejection ( 1 , 2 , 3 ). (aai.org)
It is used for the prophylaxis of graft rejection in organ and tissue transplantation. (lookformedical.com)

Pancreas Transplantation Pancreas transplantation is a form of pancreatic beta-cell replacement that can restore normoglycemia in diabetic patients. (merckmanuals.com)
In the mid-2000s, new trials were undertaken to establish protocols that enable successful IT using islets from a single donor pancreas [ 7 , 8 ]. (hindawi.com)

This in vitro model has features which appear to correlate with the islet destruction seen in vivo and could be a useful model for the study of the mechanisms underlying the rapid destruction of xenogeneic islets in primate recipients. (ox.ac.uk)

Areas related to clinical and experimental transplantation are also of interest. (lidsen.com)
However, recent clinical trials show that micro-encapsulated islets are not fully retrievable after transplantation. (lidsen.com)
Conclusion: Measurement of the glucose-stimulated respiratory activity using SECM technique is a novel method that may be useful as a rapid, potent predictor of the outcome of clinical islet transplantation. (elsevierpure.com)
Background: Clinical application of skeletal myoblast transplantation has been curtailed due to arrhythmogenicity and inconsistent therapeutic benefits observed in previous studies. (elsevierpure.com)
Islet transplantation (IT) remains a promising therapy for diabetes mellitus but current results justify its clinical use only with a small subset of type 1 diabetics. (hindawi.com)
Clinical Transplantation. (houstonmethodist.org)

Although such attempts produced no positive benefit, further research found in the mid twentieth century that human cells could be used to help prevent the human body rejecting transplanted organs, leading in time to successful bone marrow transplantation as has become common practice in treatment for patients that have compromised bone marrow after disease, infection, radiation or chemotherapy. (wikipedia.org)
in 1968, in Minnesota, the first successful human bone marrow transplantation took place. (wikipedia.org)

In conclusion, the SP hydrogel is non-cytotoxic and supports normal islet structure and function both in vitro and in vivo. (fujita-hu.ac.jp)
Specifically, the ability of the hydrogel to separate individual islets after transplantation is important for maintaining their function in vivo. (fujita-hu.ac.jp)
Ex vivo labeling of islets and imaging of posttransplant early events were feasible. (unige.ch)

16. T ljedal I-B: Some aspects of the apparent glucose-6-phsophatase activity in the pancreatic islets of mammals. (taljedal.se)
27. T ljedal I-B: Presence, inducation and possible role of glucose 6-phosphatase in mammalian pancreatic islets. (taljedal.se)
28. T ljedal I-B: Kinetics of glucose 6-phosphatase in pancreatic islets as revealed by staining histochemistry. (taljedal.se)
In vitro, rat islets in SP hydrogel maintained a 3D structure and high glucose-stimulated insulin release similar to that observed in freshly isolated islets for 4 weeks, while control islets cultured in suspension lost their 3D structure and insulin release responses by 2 weeks. (fujita-hu.ac.jp)
Sprague-Dawley rat islets were incubated for various times, with various concentrations of 2-[F]fluoro-2deoxy-D-glucose (FDG), and in presence of various glucose concentrations. (unige.ch)

These results suggest that WA could be incorporated as an adjunctive treatment to improve islet transplant outcome. (elsevierpure.com)

Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains. (lookformedical.com)
2015年11月11日, the TTS-JST Transplantation Science Mentee-Mentor Travel Award, International Basic Science Mentee-Mentor Awards-TSS2015, Bacterial pathogens provoke B cells responding to blood group A-antigens to be resistant to calcineurin inhibitors through TLR-MyD88 pathway. (hiroshima-u.ac.jp)

In Transplantation procedures, the native body part may or may not be removed and the transplanted body part may take over all or part of the function of the transplanted organ. (ahima.org)
BC is the only current method for making fully functional, three-dimensional organs from pluripotent cells and generating human organs in large mammalian hosts may be able to address the critical worldwide problem of organ shortages for transplantation [ 16 ]. (biomedcentral.com)

Continuous PO(2) measurements were made in a static incubation system to assess the role of hypoxia in islet destruction. (ox.ac.uk)

Despite being a major breakthrough, the EP often required donor islets isolated from 2-4 pancreata. (hindawi.com)

Immunosuppression must be continued lifelong or until islet cell function ceases. (merckmanuals.com)
Although further improvements may be necessary, this immunosuppression-free approach may replace intraportal islet transplantation in the near future. (lidsen.com)

Address correspondence and reprint requests to Dr. Leonard C. Harrison, Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia. (aai.org)

Its disadvantages are that transplanted glucagon -secreting alpha cells are nonfunctional (possibly complicating hypoglycemia) and several pancreata are usually required for a single islet cell recipient (exacerbating disparities between graft supply and demand and limiting use of the procedure). (merckmanuals.com)
This important property, combined with the versatility and biocompatibility, makes our SP hydrogel a promising synthetic scaffold that can facilitate transplantation of organized heterogeneous cells to preserve their micro-structure and function. (fujita-hu.ac.jp)
A dynamic islet perifusion system was used to assess the effect of islets on blood cells and coagulation factors. (ox.ac.uk)
since most of the total transplanted islet volume is accounted for by large islets, most of the intraportal islet cells are likely to be anoxic and not fully functional. (hindawi.com)
Syngeneic cells induced negligible neutrophil or Mφ responses. (aai.org)
2015年11月11日, the TTS-JST Transplantation Science Mentee-Mentor Travel Award, International Basic Science Mentee-Mentor Awards-TSS2015, Propagated NK cells from human pluripotent stem cells exert vigorous cytotoxicity against HCC and inhibit HCV replication. (hiroshima-u.ac.jp)

additional injections of islet preparations may be necessary to obtain longer-lasting insulin independence. (merckmanuals.com)

collagenase is infused into the pancreatic duct to separate islets from pancreatic tissue. (merckmanuals.com)

Pediatric Transplantation. (bcm.edu)

Therefore, we need reliable, rapid methods enabling accurate prediction of islet quality. (elsevierpure.com)

Herein, we report a synthetic saccharide-peptide (SP) hydrogel that allows suspending islets in liquid and injecting for in situ polymerization without forming islet clumps, indicating its potential in extrahepatic islet transplantation. (fujita-hu.ac.jp)

Oxygenation of the intraportally transplanted islet has not been studied extensively. (hindawi.com)

Transplantation Proceedings , 41 (1), 311-313. (elsevierpure.com)
Transplantation Proceedings, 23 (6). (pitt.edu)

Anatomy30

Organisms21

Diseases48

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment91

Phenomena and Processes31

Disciplines and Occupations1

Information Science3

Named Groups3

Health Care18

Allogeneic9

Pancreatic islet2

Kidney6

Rats8

Evaluating islet potency1

Mouse islets8

Subcutaneously2

Liver8

Grafts3

Transplants1

Human Islets2

Numbers of syngeneic1

Mice7

Type 1 diabet3

Cell transplantation6

Outcomes1

Induces1

Survival1

Engraftment4

Subcutaneous7

Allografts2

Viability1

Rejection3

Pancreas2

Recipients1

Clinical6

Bone2

Vivo3

Glucose5

Outcome1

Antigens2

Organ2

Hypoxia1

Donor1

Immunosuppression2

Autoimmunity1

Cells6

Preparations1

Tissue1

Pediatric1

Methods1

Situ1

Oxygenation1

Proceedings2