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  • donor
  • Enucleation efficiency and efficiency of fusion with somatic cell (nucleus donor) were compared. (deepdyve.com)
  • 2. Micro-surgical dissection of the donor strip must be perfomed in a professional manner: this is essential for successful transplantation. (wellnesskliniek.com)
  • FUE hair transplantation is a technique whereby follicular units are removed from the donor area using a small punch instrument. (wellnesskliniek.com)
  • The National Marrow Donor Program website (www.marrow.org) lists quite a number of diseases currently treatable by stem cell transplants, including acute and chronic leukemias, Hodgkin's Disease and Non-Hodgkin's Lymphoma, sickle cell disease, and various cancers. (illinoisrighttolife.org)
  • M-CSF treatment resulted in an increased production of mature myeloid donor cells and an increased survival of recipient mice infected with lethal doses of clinically relevant opportunistic pathogens, namely the bacteria Pseudomonas aeruginosa and the fungus Aspergillus fumigatus . (rupress.org)
  • The transfer of HAdV-specific donor T cells in patients with HAdV viremia has been evaluated in several clinical phase I/II trials and preliminary data suggest that this approach may be feasible and effective. (springer.com)
  • They used a bit of skin from each man, took the DNA from the skin cells and inserted it into the egg cell of a female donor, and grew very early embryos called blastocysts, the team reports in the journal Cell Stem Cell. (bioethics.net)
  • However, these problems associated with histocompatibility may be solved using autologous donor adult stem cells, therapeutic cloning. (wikipedia.org)
  • Embryonic stem cells provide hope that it will be possible to overcome the problems of donor tissue shortage and also, by making the cells immunocompatible with the recipient. (wikipedia.org)
  • For example, a female DNA donor would be the clone's genetic twin, rather than mother, complicating the genetic and social relationships between mother and child as well as the relationships between other family members and the clone. (wikipedia.org)
  • This therapy would also eliminate the need for donor hair, as it can be simply grown from the patient's own cells. (wikipedia.org)
  • Texas A&M University cloned a Black Angus bull named 86 Squared in 2000, after cells from his donor, Bull 86, had been frozen for 15 years. (wikipedia.org)
  • The technique consists of taking an enucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)
  • The nucleus of the donor egg cell is removed and discarded, leaving it 'deprogrammed. (wikipedia.org)
  • These cells genetically matched the donor organism from which they came.This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research. (wikipedia.org)
  • The resulting cells would be genetically identical to the somatic cell donor, thus avoiding any complications from immune system rejection. (wikipedia.org)
  • In 1997, innovations in frogs, Xenopus laevis, by developmental biologist Jonathan Slack of the University of Bath, created headless tadpoles, which could allow future applications in donor organ transplantation. (wikipedia.org)
  • fetal
  • NK cells obtained by exposing mouse fetal thymocytes to appropriate combinations of IL-4, IL-2, and PMA are phenotypically indistinguishable from cultured adult splenic NK cells with the exception that they generally lack measurable expression of all of the inhibitory Ly49 molecules that can currently be detected with Abs (Ly49A, -C, -G, and -I) and of the activating molecule Ly49D. (jimmunol.org)
  • Despite this deficiency, fetal NK cells have a similar specificity to Ly49-expressing adult splenic NK cells. (jimmunol.org)
  • Individual fetal NK cell clones display an essentially invariant and broad specificity similar to that of polyclonal populations of fetal or adult NK cells, although significant differences in the fine specificity of clones can occasionally be detected. (jimmunol.org)
  • The expression of individual variable Ags is independent and stochastic, resulting in fetal NK "clones" being potentially composed of hundreds of phenotypically distinct cells. (jimmunol.org)
  • We hypothesize that fetal NK cells behave as progenitor cells that are undergoing a process of rapid, extensive, and continuous diversification and that are individually capable of generating and regenerating a complex NK cell repertoire. (jimmunol.org)
  • Only one group has ever succeeded, and their lines were generated using fetal and infant cells. (bioethics.net)
  • Researchers were able to develop a novel way to transplant human fetal kidneys into anephric rats to overcome a significant obstacle in impeding human fetal organ transplantations. (wikipedia.org)
  • 2001
  • In 2001, he was also the first to clone an endangered species (a Gaur), and in 2003, he cloned an endangered wild ox (a Banteng) from the frozen skin cells of an animal that had died at the San Diego Zoo nearly a quarter-of-a-century earlier. (wikipedia.org)
  • In 1989, he was a founding member of the Wellcome/CRC Institute for Cell Biology and Cancer (later Wellcome/CR UK) in Cambridge, and was its Chair until 2001. (wikipedia.org)
  • In 2001, scientists at Texas A&M University created the first cloned cat, CC (CopyCat). (wikipedia.org)
  • In 2001 researchers at Advanced Cell Technology in Worcester, Massachusetts, United States, reported that 24 successfully cloned Holsteins had been monitored from birth to the age of four. (wikipedia.org)
  • A purebred Hereford calf clone named Chloe was born in 2001 at Kansas State University's purebred research unit. (wikipedia.org)
  • Millie and Emma were two female Jersey cows cloned at the University of Tennessee in 2001. (wikipedia.org)
  • In 2001, Brazil cloned their first heifer, Vitória. (wikipedia.org)
  • genes
  • Gene expression analysis of the freshly sorted Ebf2(+) cells demonstrated the expression of genes previously reported to be associated with MSCs and the coexpression of multiple lineage-associated genes at the single-cell level. (diva-portal.org)
  • Methods Single synovial tissue CD19+cells were Fluorescence Activated Cell Sorting (FACS)-sorted and V H /V L Ig genes cloned to generate recombinant monoclonal antibodies (rmAbs) from patients with ELS+/ACPA+RA. (bmj.com)
  • Both the frequency of cells expressing a given Ly49 molecule and the level of expression are influenced by host MHC genes ( 6 , 7 , 8 ). (jimmunol.org)
  • In the mouse, the NK1.1 Ag, which is a homologue of NKRP1 ( 15 ), is expressed on all NK cells in appropriate strains ( 16 ), is encoded by genes in the NK complex ( 4 ), and may be involved in recognition as judged by the ability of Ab to NK1.1 to induce or enhance killing by NK cells ( 17 ). (jimmunol.org)
  • The expression 'pattern' of genes related to pluripotency in Muse cells was almost the same as that in ES and iPS cells, while the expression 'level' was much higher in ES and iPS cells and that in Muse cells. (wikipedia.org)
  • In contrast, genes related to cell cycle progression and tumorigenicity in Muse cells were at the same level as those in somatic cells, while the same genes were very high in ES and iPS cells. (wikipedia.org)
  • Genetics deals with the molecular structure and function of genes, and gene behavior in context of a cell or organism (e.g. dominance and epigenetics), patterns of inheritance from parent to offspring, and gene distribution, variation and change in populations. (wikipedia.org)
  • A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)
  • For example, if a person with Parkinson's disease donated his or her somatic cells, the stem cells resulting from SCNT would have genes that contribute to Parkinson's disease. (wikipedia.org)
  • Michael Houghton - co-discoverer of hepatitis C Tak Wah Mak - first to identify and clone T-cell receptor genes Joseph B. Martin (MD '62) - member of the team that discovered a biomarker that led to locating the gene associated with Huntington's disease. (wikipedia.org)
  • tissue
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (biologists.org)
  • The laser burns result in tissue loss around the transplantation holes. (wellnesskliniek.com)
  • Because of their plasticity and potentially unlimited capacity for self-renewal, embryonic stem cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. (wikipedia.org)
  • According to a 2002 article in PNAS, "Human embryonic stem cells have the potential to differentiate into various cell types, and, thus, may be useful as a source of cells for transplantation or tissue engineering. (wikipedia.org)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
  • In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikipedia.org)
  • Muse cells are shown to home into the damage site and spontaneously differentiate into tissue-compatible cells according to the microenvironment to contribute to tissue regeneration when infused into the blood stream. (wikipedia.org)
  • graft versus
  • When given on days 3 and 4 after HLA-haploidentical T-cell replete blood or marrow transplantation, high-dose cyclophosphamide depletes dividing alloreactive T-cells, resulting in low rates of acute graft -versus- host disease, comparable to HLA-matched transplantation, and chronic graft -versus- host disease incidence below that seen with T-cell replete HLA-matched transplantation. (springer.com)
  • Effect of HLA incompatibility on graft-versus-host disease, relapse, and survival after marrow transplantation for patients with leukemia or lymphoma. (springer.com)
  • Effector memory CD4+ T cells mediate graft-versus-leukemia without inducing graft-versus-host disease. (springer.com)
  • organs
  • In some organs, such as the gut and bone marrow, stem cells regularly divide to repair and replace worn out or damaged tissues. (illinoisrighttolife.org)
  • After the surgeons remove the organs, they are transported as quickly as possible to the recipient, for immediate transplantation. (wikipedia.org)
  • Opponents of cloning have concerns that technology is not yet developed enough to be safe, that it could be prone to abuse (leading to the generation of humans from whom organs and tissues would be harvested), and have concerns about how cloned individuals could integrate with families and with society at large. (wikipedia.org)
  • Serious ethical concerns have been raised by the future possibility of harvesting organs from clones. (wikipedia.org)
  • Advocates of human therapeutic cloning believe the practice could provide genetically identical cells for regenerative medicine, and tissues and organs for transplantation. (wikipedia.org)
  • Such cells, tissues, and organs would neither trigger an immune response nor require the use of immunosuppressive drugs. (wikipedia.org)
  • derivation
  • However, the derivation of such cell types from ESs is not without obstacles and hence current research is focused on overcoming these barriers. (wikipedia.org)
  • scientists
  • Human cloning is the term used by scientists to describe the process of creating new life by making duplicates of biological material. (illinoisrighttolife.org)
  • You will hear from Museum scientists, medical researchers at the frontiers of the field, and a panel of bioethics experts who will address the ethical implications of stem cell research and therapy. (coursera.org)
  • This week, New York University's Dr. Esteban Mazzoni will discuss how scientists can coax stem cells to differentiate into particular cell types. (coursera.org)
  • The first time scientists were able to grow artificial hair follicles from stem cells was in 2010. (wikipedia.org)
  • Scientists of the Berlin Technical University in Germany took animal cells and created the follicles by using them. (wikipedia.org)
  • In 2011 scientists from the University of Pennsylvania School of Medicine published their own findings regarding hair cloning. (wikipedia.org)
  • A cloned male buffalo calf Shresth was born in 2010 at the NDRI In May 2010, Got became the first cloned Spanish Fighting Bull, cloned by Spanish scientists. (wikipedia.org)
  • In July 2016 scientists at the National University Toribio Rodríguez de Mendoza in Chachapoyas, Peru cloned a Jersey cattle by handmade cloning method using cells of an ear of a cow. (wikipedia.org)
  • Leading scientists to seek an alternative method of obtaining stem cells, SCNT is one such method. (wikipedia.org)
  • islet
  • Ray Rajotte - international diabetes leader, renown for work related to the Edmonton Protocol islet transplantation procedure James Shapiro - member of the team that pioneered the Edmonton Protocol and continues to work to improve islet transplants Richard Fedorak - internationally renowned gastroenterologist specializing in inflammatory bowel disease. (wikipedia.org)
  • frog
  • Anatomy and Development: images, fate maps, and videos Community Link --- jobs, labs which study Xenopus Protocol List- identify clones, antibodies, procedures Stock Centre- The National Xenopus Resource (maintains frog stocks, offers advanced research training) The Nobel Prize for Medicine or Physiology was awarded to John B. Gurdon and Shinya Yamanaka on October 8, 2012. (wikipedia.org)
  • It seems it is much harder to clone a human being than it is to clone a sheep, a frog or a mouse. (bioethics.net)
  • embryonic cell
  • A technique appropriate to investigation of the developmental fates of distinct embryonic cell types is described and the fate of a particular type of chick myoblast (CMR-I) examined. (biologists.org)
  • immunosuppressive
  • The main risk factor for HAdV infection is prolonged immunosuppression, attributable either to in vivo or ex vivo T-cell depletion, prolonged lymphopenia, or to GvHD-associated immunosuppressive posttransplant therapy. (springer.com)
  • Mice
  • Primed allospecific T cells prevent the effects of costimulatory blockade on prolonged cardiac allograft survival in mice. (springer.com)
  • Induction of alloreactive cytotoxic T cells by acute virus infection of mice. (springer.com)
  • Unlike ES and iPS cells, transplanted Muse cells in testes of immunodeficient mice -a commonly used experiment to test the tumorigenicity of stem cells- have not been reported to form teratomas, even after six months. (wikipedia.org)
  • Handmade
  • Handmade cloning (HMC) technique has been applied as efficient and cost‐effective alternative in many livestock species. (deepdyve.com)
  • embryonic
  • In vitro model systems, based on the differentiation of embryonic stem (ES) cells, provide an alternative means of identifying and characterizing these populations. (biologists.org)
  • Transfected cells are then plated onto a layer of irradiated mouse embryonic fibroblasts (iMEFs) for continued reprogramming. (jove.com)
  • Once iPSC colonies exhibit typical human embryonic stem cell (hESC) morphology, they are gently transferred to individual iMEF-coated tissue culture plates for continued growth and expansion. (jove.com)
  • This was the first in a series of development that have begun to make rats tractable as genetic research subjects, although they still lag behind mice, which lend themselves better to the embryonic stem cell techniques typically used for genetic manipulation. (wikipedia.org)
  • this approach has been championed as an answer to the many issues concerning embryonic stem cells (ESC) and the destruction of viable embryos for medical use, though questions remain on how homologous the two cell types truly are. (wikipedia.org)
  • The blastocyst stage is developed by the egg which helps to create embryonic stem cells from inner cell mass of the blastocyst.The first animal that is developed by this technique is Dolly, the sheep in 1996. (wikipedia.org)
  • Embryonic stem cells are undifferentiated cells of an embryo. (wikipedia.org)
  • In the United States, scientists at the Harvard Stem Cell Institute, the University of California San Francisco, the Oregon Health & Science University, Stemagen (La Jolla, CA) and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. (wikipedia.org)
  • He is Uehiro Professor of Practical Ethics at the University of Oxford, Fellow of St Cross College, Oxford, Director of the Oxford Uehiro Centre for Practical Ethics, Sir Louis Matheson Distinguished Visiting Professor at Monash University, and Head of the Melbourne-Oxford Stem Cell Collaboration, which is devoted to examining the ethical implications of cloning and embryonic stem cell research. (wikipedia.org)
  • limb
  • He discovered evidence for the relationship between somites and spinal ganglia, that transplanted limbs can be controlled by foreign ganglia, and the plasticity of ganglia in response to limb transplantations. (asu.edu)
  • bone marrow
  • We investigated whether AM enhances bone marrow cell-induced angiogenesis. (ahajournals.org)
  • Methods and Results- Immediately after hindlimb ischemia was created, rats were randomized to receive AM infusion plus bone marrow-derived mononuclear cell (MNC) transplantation (AM+MNC group), AM infusion alone (AM group), MNC transplantation alone (MNC group), or vehicle infusion (control group). (ahajournals.org)
  • Transplantation of bone marrow-derived MNCs and infusion of AM were performed in 40 rats immediately after hindlimb ischemia was created. (ahajournals.org)
  • Bradley TR, Metcalf D. The growth of mouse bone marrow cells in vitro. (springer.com)
  • To address these questions, F1 (H-2βξδ) chimeric mice were either reconsti- binary options brokers minimum trade with parental spleen or bone marrow-derived cells61 binary options brokers minimum trade served as donors of these cells to H-2βξδ recipients. (imbc-med.ru)
  • It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • this disorder is a form of plasma cell dyscrasia in which no myeloma protein is detected in serum or urine (at least as determined by conventional laboratory methods) of individuals who have clear evidence of an increase in clonal bone marrow plasma cells and/or evidence of clonal plasma cell-mediated tissue injury (e.g. plasmacytoma tumors). (wikipedia.org)
  • This is in contrast to Shwachman-Bodian-Diamond syndrome, in which the bone marrow defect results primarily in neutropenia, and Fanconi anemia, where all cell lines are affected resulting in pancytopenia. (wikipedia.org)
  • A diagnosis of DBA is made on the basis of anemia, low reticulocyte (immature red blood cells) counts, and diminished erythroid precursors in bone marrow. (wikipedia.org)
  • Chronic lymphocytic leukemia (CLL) is a type of cancer in which the bone marrow makes too many lymphocytes (a type of white blood cell). (wikipedia.org)
  • CLL results in the build up of B cell lymphocytes in the bone marrow, lymph nodes, and blood. (wikipedia.org)
  • In some individuals, the disease comes to light only after the cancerous cells overwhelm the bone marrow resulting in anemia producing tiredness or weakness. (wikipedia.org)
  • The diagnosis of CLL is based on the demonstration of an abnormal population of B lymphocytes in the blood, bone marrow, or tissues that display an unusual but characteristic pattern of molecules on the cell surface. (wikipedia.org)
  • lymphoid
  • We could predict with high accuracy (greater than or equal to 80 %) a wide range of immunological statuses such as healthy, transplantation recipient, and lymphoid cancer, suggesting as a proof of principle that diversity profiling can recover a large amount of immunodiagnostic fingerprints from immune repertoire data. (beds.ac.uk)
  • In other cases, however, the fusion, while occurring in myeloid precursor cells, promotes proliferation and differentiation of precursor cells along the neutrophil linage or, less commonly, occurs in lymphoid precursor cells to promote the proliferation and differentiation of precursor cells along the lymphoid lineage. (wikipedia.org)
  • receptor
  • Resulting iPSCs were further characterized and deemed free of transfected DNA, integrated transgene DNA, and lack detectable gene rearrangements such as those within the immunoglobulin heavy chain and T cell receptor loci of more differentiated cell types. (jove.com)
  • When an antigen-presenting cell interacts with a T cell receptor on T cells, there is an increase in the cytoplasmic level of calcium, which activates calcineurin, by binding a regulatory subunit and activating calmodulin binding. (wikipedia.org)
  • The protein encoded by this gene is one of two proteins that are required to form the DQ heterodimer, a cell surface receptor essential to the function of the immune system. (wikipedia.org)
  • genome
  • As they are stimulated to become plasma cells, B cells refashion parts of their genome in efforts to create a new gene that encodes a functional antibody. (wikipedia.org)
  • Development will ensue normally and after many mitotic divisions, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). (wikipedia.org)
  • However, they are completely different in their purposes, as gene therapy aims to cure a defect in cells, and transgenesis seeks to produce a genetically modified organism by incorporating the specific transgene into every cell and changing the genome. (wikipedia.org)
  • precursors
  • Conti L, Gessani S. GM-CSF in the generation of dendritic cells from human blood monocyte precursors: recent advances. (springer.com)
  • Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
  • At the other end of this spectrum, detection of the myeloid protein indicates the presence of a hematological malignancy, i.e. multiple myeloma, Waldenström's macroglobulinemia, or other B cell-associated neoplasm, that derives stepwise from its MGUS precursors. (wikipedia.org)
  • induces
  • Thus, in X. laevis embryos, the ectoderm is patterned by the secretion of BMP antagonists from the dorsal blastopore lip, or Spemann's organizer: BMP signalling is active ventrally, and induces the formation of surface ectoderm, whereas dorsal BMP signalling is inhibited, and the cells adopt a neural fate ( Hemmati-Brivanlou and Melton, 1997 ). (biologists.org)
  • Background- Previous studies have shown that adrenomedullin (AM) inhibits vascular endothelial cell apoptosis and induces angiogenesis. (ahajournals.org)
  • Proteins
  • DNA sequences comprising nucleic acids encoding fusion proteins comprising an Fc portion of an antibody attached at the N-terminus of an OB protein moiety, vectors comprising such DNA sequences, host cells comprising such vectors or DNA sequences, and processes for preparing such fusion proteins, and. (google.com)
  • The overproduced Ig's, termed myeloma proteins, commonly circulate in blood, may accumulate in urine, and are the hallmarks of plasma cell dyscrasias including their most malignant forms viz. (wikipedia.org)
  • When bound by its proper ligand, Platelet-derived growth factor (PDGF), it [tyrosine kinase]] becomes active in phosphorylating proteins that, among other functions, promote cell growth and proliferation. (wikipedia.org)
  • These have been shown to interact in a series of cell-cell and cell-matrix signaling proteins. (wikipedia.org)
  • In a study of patients with a disease similar to Conorenal (renal-retinal Senior-Loken) the authors state "We show that nephrocystin-5, RPGR and calmodulin can be coimmniprecipitated from retinal extracts, and that these proteins localize to connecting cilia of photoreceptors and to primary cilia of renal epithelial cells. (wikipedia.org)
  • therapeutic
  • The ability to progress via a definitive ectoderm intermediate will underpin the development of rational and efficient methodologies for the derivation of specific ectodermal cell populations from pluripotent cells in culture for use as experimental models or to produce cells with therapeutic applications. (biologists.org)
  • When these findings are taken together, combination therapy with MNC transplantation and AM infusion may have additional or synergetic effects on therapeutic angiogenesis for the treatment of severe peripheral vascular disease. (ahajournals.org)
  • It is used in both therapeutic and reproductive cloning. (wikipedia.org)
  • Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term. (wikipedia.org)
  • pluripotent cells
  • The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo. (wikipedia.org)
  • These cells genetically matched the donor organism from which they came.This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research. (wikipedia.org)
  • peripheral
  • This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using episomal plasmids and histone deacetylase (HDAC) inhibitors. (jove.com)
  • Injection of CD34+ hematopoietic Stem Cells has been clinically applied to treat various diseases including Spinal Cord Injury, Liver Cirrhosis and Peripheral Vascular disease. (wikipedia.org)
  • infection
  • He is also the Associate Director of the Institute for Immunity, Transplantation and Infection ITI at Stanford and Director of the Center for Clinical Immunology at Stanford (CCIS). (wikipedia.org)
  • diseases
  • Potential uses of human iPSCs include modeling pathogenesis of human genetic diseases, autologous cell therapy after gene correction, and personalized drug screening by providing a source of patient-specific and symptom relevant cells. (jove.com)
  • Like the described Kidney diseases, Retinitis Pigmentosa is a disease where the ciliar cells (Rods and Cones) fail to thrive. (wikipedia.org)
  • In other words, there is a common link between certain kidney diseases and some forms of RP and it is through the something related to a problem in the cilia cells. (wikipedia.org)
  • stem cells
  • A few years ago, the establishment of human induced pluripotent stem cells (iPSCs) ushered in a new era in biomedicine. (jove.com)
  • Dr. Mark C. Walters of UCSF Benioff Children's Hospital Oakland has been the lead researcher for the Northstar Study (HGB-204): A Phase I/II Study of Gene Therapy for Beta-Thalassemia Major via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex-Vivo with a Lentiviral Beta AT87Q-Globin Vector (LentiGlobin BB305 Drug Product). (healthcanal.com)
  • LentiGlobin BB305, produced by BlueBird Bio, uses a non-communicable virus to deliver a fully functioning HBB gene to a patient's own blood-producing stem cells. (healthcanal.com)
  • This Phase I/II study has examined if using LentiGlobin BB305 to introduce a fully functioning hemoglobin gene into a patient's stem cells was a safe and effective treatment. (healthcanal.com)
  • Dr. Walters collected stem cells from transfusion-dependent beta-thalassemia major patients, inserted the healthy gene, and then infused the cells back into the patient after first giving high-dose chemotherapy to destroy the thalassemia-producing blood cells. (healthcanal.com)
  • It is important to mention that Long-Term Hematopoietic Stem Cells (LT-HSCs) in mice and humans are the hematopoietic cells with the greatest self-renewal capacity. (wikipedia.org)
  • citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • i.e., they are multipotential hemopoietic stem cells. (wikipedia.org)
  • This ability allows stem cells to create any cell type, which could then be transplanted to replace damaged or destroyed cells. (wikipedia.org)
  • Leading scientists to seek an alternative method of obtaining stem cells, SCNT is one such method. (wikipedia.org)
  • lineage
  • Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. (biologists.org)
  • Foxp3 is a specific marker of natural T regulatory cells (nTregs, a lineage of T cells) and adaptive/induced T regulatory cells (a/iTregs), also identified by other less specific markers such as CD25 or CD45RB. (wikipedia.org)
  • While CD4+ cells are heavily regulated and require multiple transcription factors such as STAT-5 and AhR in order to become active and function properly, Foxp3 has been identified as the master regulator for Treg lineage. (wikipedia.org)
  • cancerous cells
  • For example, patients with tumors have a local relative excess of Foxp3 positive T cells which inhibits the body's ability to suppress the formation of cancerous cells. (wikipedia.org)
  • mice
  • The purpose of the present study is to produce model mice in which the hematopoiesis supporting cells are easily distinguished from other stromal cell component. (nii.ac.jp)
  • cells in diabetic mice, and that the anti-hyperglycemic efficacy of this combination was better than that of 1-deoxynojirimycin (DNJ) or polysachharide alone. (jove.com)
  • renal disease
  • The induction or administration of Foxp3 positive T cells has, in animal studies, led to marked reductions in (autoimmune) disease severity in models of diabetes, multiple sclerosis, asthma, inflammatory bowel disease, thyroiditis and renal disease. (wikipedia.org)
  • rats
  • The heterodimer also stimulated cAMP production and thymidine incorporation by cultured thyroid cells and increased serum thyroxine levels in TSH-suppressed rats in vivo. (jci.org)
  • transfusions
  • Interim results also showed that all but one patient with a non-β0/β0 genotype (12 of 13 patients) stopped receiving regular red blood cell (RBC) transfusions, with a median time since last transfusion of 27 months. (healthcanal.com)
  • helper
  • The amount of IL-2 being produced by the T-helper cells is believed to influence the extent of the immune response significantly. (wikipedia.org)
  • This means that the use of regulatory T cells in therapy may be risky, as the T regulatory cell transferred to the patient may change into T helper 17 (Th17) cells, which are pro-inflammatory rather than regulatory cells. (wikipedia.org)
  • lymph nodes
  • CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
  • Less commonly, CLL may present with enlarged lymph nodes without a high white blood cell count or no evidence of the disease in the blood. (wikipedia.org)
  • protein
  • Suppression of STAT3 NH 2 -terminal domain chemosensitizes medulloblastoma cells by activation of protein inhibitor of activated STAT3 via de-repression by microRNA-21. (nih.gov)
  • Cotransfection of an episome that expresses enhanced green fluorescent protein (eGFP) allows for easy identification of transfected cells. (jove.com)
  • Granulysin is a protein present in cytotoxic granules of cytotoxic T cells and natural killer cells. (wikipedia.org)
  • Granulysin is a member of the saposin-like protein (SAPLIP) family and is released from cytotoxic T cells upon antigen stimulation. (wikipedia.org)
  • Hematopoietic progenitor cell antigen CD34 also known as CD34 antigen is a protein that in humans is encoded by the CD34 gene. (wikipedia.org)
  • Leukocyte cell-derived chemotaxin-2 (LECT2) is a protein first described in 1996 as a chemotactic factor for neutrophils, i.e. it stimulated human neutrophils to move directionally in an in vitro assay system. (wikipedia.org)
  • The protein was detected in and purified from cultures of Phytohaemagglutinin-activated human T-cell leukemia SKW-3 cells. (wikipedia.org)
  • Subsequent studies have defined LECT2 as a hepatokine, i.e. a substance made and released into the circulation by liver hepatocyte cells that regulates the function of other cells: it is a hepatocyte-derived, hormone-like, signaling protein. (wikipedia.org)
  • Using very sensitive methods, LECT2 protein can also be detected at low levels in the endothelial cells of hepatic arteries and veins including central veins. (wikipedia.org)
  • A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. (wikipedia.org)
  • vivo
  • Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. (biologists.org)
  • Typhimurium remains in the phagosomal compartment of the host binary options brokers minimum trade (unlike Shigella and Listeria which readily binary options trading competition and has been used to deliver eukaryotic expression vectors to cultured cells and in vivo. (imbc-med.ru)
  • The Foxp3 gene is also known to convert naïve T-cells to Treg cells, which are capable of an in vivo and in vitro suppressive capabilities suggesting that Foxp3 is capable of regulating the expression of suppression-mediating molecules. (wikipedia.org)
  • Conversely
  • Conversely, patients with an autoimmune disease such as systemic lupus erythematosus (SLE) have a relative dysfunction of Foxp3 positive cells. (wikipedia.org)
  • commonly
  • When these cells or tissues are subjected to inflammatory, fibrotic, and other insults, they commonly reduce their expression of LECT2. (wikipedia.org)
  • However, the cited gene changes can go awry in plasma cells by, for example, placing a gene that ordinarily controls cell growth adjacent to the normally highly active antibody gene promotor thereby creating a cancer-causing oncogene or, more commonly, by forming extra chromosomes (see trisomy) or chromosomes that have deleted or repetitive sections, any of which such changes may promote malignancy in more complex and less well understood ways. (wikipedia.org)
  • Gene Therapy
  • Transgenesis is the same as gene therapy in the sense that they both transform cells for a specific purpose. (wikipedia.org)
  • Furthermore, the enhanced precision of surgery allows additional interventions, with the application of gene therapy and stem-cell therapy to retinal dystrophies (inherited conditions). (wikipedia.org)
  • apoptosis
  • 8 Furthermore, AM has been shown to inhibit vascular endothelial cell apoptosis and induce angiogenesis through the activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. (ahajournals.org)