• CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. (thermofisher.com)
  • CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. (thermofisher.com)
  • The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
  • these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. (exbio.cz)
  • Combinations of CD45 isoforms are crucial for immune function and disease. (exbio.cz)
  • Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. (nsjbio.com)
  • This antibody will bind to all CD45 isoforms. (nsjbio.com)
  • CD45 contains a Tn antigen in exon B. CD45 has 3 important exons (4,5,6), that are designated A,B,C. Isoforms of CD45 are labeled depending on the presence of these exons. (wikipedia.org)
  • However, active T cells express shorter isoforms of CD45 (CD45RO, CD45RA) that lack exon B. CLEC10A signalling induces IL-10 production in dendritic cells, in part through increasing intracellular Ca2+ concentration. (wikipedia.org)
  • CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
  • Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15). (rndsystems.com)
  • CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). (rndsystems.com)
  • The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. (irealbio.com)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (thermofisher.com)
  • CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). (rndsystems.com)
  • Leukocyte differentiation antigens often represent important markers for the diagnosis, classification, prognosis, and therapeutic targeting of myeloid leukemia. (biomedcentral.com)
  • Herein, we report a potential leukocyte differentiation antigen gene VSTM1 ( V-set and transmembrane domain-containing 1 ) that was downregulated in bone marrow cells from leukemia patients and exhibited a higher degree of promoter methylation. (biomedcentral.com)
  • Therefore, VSTM1-v1 might represent an important myeloid leukocyte differentiation antigen and provide a potential target for the diagnosis and treatment of leukemia. (biomedcentral.com)
  • VSTM1 ( V-set and transmembrane domain-containing 1 ) encodes a potential leukocyte differentiation antigen that is highly expressed in myeloid cells, but silenced in multiple leukemia cell lines [ 1 ]. (biomedcentral.com)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • dashed line histogram) or PerCP-Cy5.5 Rat Anti-Mouse CD45 antibody (Cat. (bdbiosciences.com)
  • IHC testing of FFPE human tonsil with anti-CD45 antibody (clone SPM570). (nsjbio.com)
  • This anti-CD45 antibody is available for research use only. (nsjbio.com)
  • Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (nsjbio.com)
  • Due to variation in protocol and secondary antibody used, the anti-CD45 antibody may need to be titered for optimal performance. (nsjbio.com)
  • Neoplastic cells from a T cell lymphoma were used as the immunogen for the anti-CD45 antibody. (nsjbio.com)
  • Anti-CD45 antibody (with azide) can be stored at 2-8oC. (nsjbio.com)
  • The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45. (biolegend.com)
  • 1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
  • Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. (thermofisher.com)
  • The HIT2 antibody reacts with CD38, an ~45 kDa type II transmembrane glycoprotein expressed by many cell types, especially leukocytes. (stemcell.com)
  • Antibody FMC7 ( F linder M edical C entre) appears to recognise a conformational variant of CD20 [10] [11] also known as the FMC7 antigen. (wikidoc.org)
  • CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (nsjbio.com)
  • CLEC10A is a type II transmembrane protein (passing one time through the membrane and oriented with the N terminus inward) that induces endocytosis after ligand binding. (wikipedia.org)
  • It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). (rndsystems.com)
  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (neobiotechnologies.com)
  • CD47 ( C luster of D ifferentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene . (wikidoc.org)
  • CD47 interacts with signal-regulatory protein alpha (SIRPα), an inhibitory transmembrane receptor present on myeloid cells . (wikidoc.org)
  • Immunoadhesin structure The majority of immunoadhesins combine the hinge and Fc regions of an IgGl heavy chain with the extracellular domain (ECU) of a type I transmembrane protein, usually a receptor or an adhesion molecule (Fig. 1). (coek.info)
  • The protein has no known natural ligand [4] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. (wikidoc.org)
  • The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. (rndsystems.com)
  • CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). (rndsystems.com)
  • FAs are structures composed of clustered transmembrane proteins called integrins that bind to the extracellular matrix and link to the actin cytoskeleton and control cell migration, proliferation, survival and differentiation (Gardel et al. (bio2009.org)
  • CD47 is a 50 kDa membrane receptor that has extracellular N-terminal IgV domain , five transmembrane domains , and a short C-terminal intracellular tail. (wikidoc.org)
  • Exons 1, 2 and most of exon 3 encode the N-terminal extracellular domain while the remainder of exon 3 and exon 4 encode transmembrane and cytoplasmic domains. (wikidoc.org)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (thermofisher.com)
  • The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. (irealbio.com)
  • B-lymphocyte antigen CD20 or CD20 is an activated- glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase ( CD45 R+, CD117 +) and progressively increasing in concentration until maturity. (wikidoc.org)
  • Iron nanoparticles are conjugated with two types of antibodies (one against antigens on therapeutic cells and the other directed at injured cells) to produce magnetic bifunctional cell engager (MagBICE). (regenerativemedicine.net)
  • Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
  • CD45 isoform expression can change in response to cytokines. (biolegend.com)
  • CD45RB is a 180-240 kD single chain type I membrane glycoprotein. (biolegend.com)
  • A ubiquitously expressed membrane glycoprotein. (rush.edu)
  • It is an exon 5 splice variant of the tyrosine phosphatase CD45. (biolegend.com)
  • CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (abeomics.com)
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. (thermofisher.com)
  • Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. (exbio.cz)
  • CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). (rndsystems.com)
  • Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD16 PE and CD14 Super Bright 780. (thermofisher.com)
  • We observed higher CD45-/large-size cells and lower CD8+ T cell percentages in MA than PW. (bvsalud.org)
  • CD45-/large-size cells were morphologically malignant and expressed CD274 (PD-L1), CD252 (OX40L), and EGFR. (bvsalud.org)
  • CD47 is a high affinity receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis, and in this later capacity the TSP1-CD47 interaction inhibits nitric oxide signaling at multiple levels in vascular cells. (wikidoc.org)
  • Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
  • In molecular biology , CD4 ( cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells , monocytes , macrophages , and dendritic cells . (wikidoc.org)
  • T cells displaying CD4 molecules (and not CD8 ) on their surface, therefore, are specific for antigens presented by MHC II and not by MHC class I (they are MHC class II-restricted ). (wikidoc.org)
  • CD4 is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells . (wikidoc.org)
  • When an OV is armed with bi-specific T cell engager (BiTE), it also expresses and secretes a large quantity of BiTEs from infected cells, which can diffuse throughout the tumor tissue, activating T cells to recognize and kill cancer cells antigen-specifically. (mdpi.com)
  • We treat acute myocardial infarction by targeting exogenous bone marrow-derived stem cells (expressing CD45) or endogenous CD34-positive cells to injured cardiomyocytes (expressing myosin light chain. (regenerativemedicine.net)
  • Generally, C-type lectins bind carbohydrate moieties usually in the presence of Ca2+ and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. (wikipedia.org)
  • CD45 has also been reported to bind galectin-1. (biolegend.com)
  • The TCR complex and CD4 each bind to distinct regions of the antigen-presenting MHCII molecule - α1/β1 and β2, respectively. (wikidoc.org)
  • The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. (irealbio.com)
  • A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. (wakehealth.edu)
  • 2012). APPL1 is recruited by Rab5 to endosomes involved in traffic of transmembrane receptors including integrins (Miaczynska et al. (bio2009.org)
  • These transmembrane receptors are not grouped into the NTR family based on sequence homology, but because they share a conserved signalling pathway utilizing the same signalling motifs. (encyclopedia.pub)
  • Here we review the interactions of metabolism, galectins and glycoprotein ligands as well as the utility of this model to predict and treat inflammation and autoimmunity. (gr.jp)
  • Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Ab (2B11). (abeomics.com)
  • CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions. (thermofisher.com)
  • Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
  • CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. (biolegend.com)
  • In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). (rndsystems.com)
  • Antigens, CD47" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
  • This graph shows the total number of publications written about "Antigens, CD47" by people in this website by year, and whether "Antigens, CD47" was a major or minor topic of these publications. (rush.edu)
  • Below are the most recent publications written about "Antigens, CD47" by people in Profiles. (rush.edu)
  • CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. (thermofisher.com)
  • Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation. (nsjbio.com)
  • Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). (rndsystems.com)
  • Metabolic regulation of UDP-GlcNAc supply to Golgi N-glycan remodeling regulates glycoprotein affinities for galectins -and in turn, trafficking and presentation at the cell surface. (gr.jp)
  • CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. (stemcell.com)
  • CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. (thermofisher.com)
  • CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. (biolegend.com)
  • Results We identified 696 MM cell surface N -glycoproteins by CSC, and developed 73 targeted MS detection assays. (bmj.com)
  • The galectin lattice is a multi-valent interaction of galectins with glycoproteins at the cell surface that displays rapid exchange of binding partners with properties of liquid-liquid phase transitions, thereby acting as an intermediary between freely diffusing glycoproteins and stable complexes in the membrane. (gr.jp)
  • However, it has been difficult to obtain human mAbs, especially those directed at human antigens, for at least two reasons. (coek.info)