And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. (rndsystems.com)
Over-expressed NSP 5a3a in HN30 cells induced a significant degree of apoptosis, an average of a 10.85 fold increase compared to controls 3 days post-transfection. (oncotarget.com)
Though, the apoptosis induced in the WI38 control cell line showed an average of a 13.2 fold increase between treated and controls comparable to the HN30 cell line 3 days post-transfection. (oncotarget.com)
Molecular analysis indentified a novel p73 dependent mechanism independent of p53 and caspase 3 activity through which NSP 5a3a is inducing apoptosis. (oncotarget.com)
E2F1 (UniprotKB: Q01094), belongs to the E2F family of transcription factor (TF), has a central role in regulating cell cycle progression and apoptosis [ 11 , 12 ] and, therefore, E2F1 activity is tightly governed by multiple feedback mechanisms and by the tumour suppressor Rb. (biomedcentral.com)
TGF B as a tumor promoter TGF B acts as tumor suppressor in normal epithelium, it inhibits cell proliferation and induces apoptosis. (mirnaarray.com)
The results demonstrate that MiTMAB induced apoptosis occurs largely following cytokinesis failure. (parp1inhibitor.com)
As anticipated, immediately after a 48 h deal with ment period, OcTMAB induced apoptosis in G2 M syn chronized cells, as evident by an increase within the percentage of cells with 2N DNA written content. (parp1inhibitor.com)
In the present study, we observed that inactivation of the ERK1/2 signaling pathway using ABT-737 dramatically increased the expression of pro-apoptotic protein Bim via transcriptional and/or posttranslational regulation, in a cell type-dependent manner, inducing mitochondria-mediated apoptosis of human oral cancer cells. (oncotarget.com)
This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. (rndsystems.com)
The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
Low levels of c Myc make it possible for for TGF B induced tran scription of p15INK4B and p21CIP1 genes. (mirnaarray.com)
Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
In contrast, nonpathogenic SIVagm infection induced DNA methylation in genes coding for regulatory proteins such as LAG-3, arginase-2, interleukin-21 and interleukin-31. (biomedcentral.com)
The combination of UHRF1 depletion and HDAC inhibition also induced marked changes in the gene expression profiles such that cell cycle-related genes were strikingly downregulated. (biomedcentral.com)
Global epigenetic adjustments in tumor cells could induce the well-documented co-expression of MAGE-A genes inside a tumor test. (kentlandsinitiative.org)
Phosphorylation3
Activation and nuclear translocation of NF-κB occurs following signal-induced phosphorylation of IκB, which leads to proteolysis via the ubiquitin pathway. (justia.com)
For IκBα, the stimulus-induced phosphorylation at serines 32 and 36 renders the inhibitor a target for ubiquitination at lysines 21 and 22, resulting in degradation. (justia.com)
The cyclinD/CDK4/6 complexes induce the phosphorylation of retinoblastoma (Rb) protein and the release of E2F, which trigger G1 cell cycle progression. (biomedcentral.com)
MRNA3
E2F1 mRNA expression was also evaluated by RT-qPCR in the melanoma cell line, SK MEL 267, before and after exposure to heat stress. (biomedcentral.com)
Our results also suggest that environmental insults, such as heat stress, could contribute to an aberrant E2F1 activity by inducing E2F1 mRNA expression. (biomedcentral.com)
Unlike miRNAs whose central function is to restrain mRNA translation by inducing degradation, lncRNAs can act as numerous roles to exert their functions by directly or indirectly interacting with DNA, RNA or protein, even can encode some short peptides (Fig. 3 a-d) [ 6 ]. (biomedcentral.com)
Chromosomal2
RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. (biolegend.com)
Cancer cells are characterized by dual DNA methylation-related changes: global hypomethylation, which can induce chromosomal instability and oncogene activation, and regional hypermethylation, which is associated with transcriptional silencing. (biomedcentral.com)
Degradation1
As a group, miRNAs are small (17-27 nucleotides) noncoding RNAs that govern gene expression in a post-transcriptional manner by binding directly to the 3′UTRs of target mRNAs, thereby repressing their translation or inducing their degradation [ 6 ]. (biomedcentral.com)
Inhibition1
AntagomiRNA-mediated inhibition of miRNA-9 increased both PP2A activity and GR nuclear translocation in macrophages, and restored steroid sensitivity in isolated macrophages and in several mouse models of steroid-resistant AHR [ 2 ] . (encyclopedia.pub)
Nuclear1
MiRNA-9 production decreased protein phosphatase 2A (PP2A) activity and also inhibited steroid-induced glucocorticoid receptor (GR) nuclear translocation. (encyclopedia.pub)
Activation3
A variety of extracellular stimuli including mitogens, cytokines, antigens, stress inducing agents, UV light and viral proteins initiate a signal transduction pathway that ultimately leads to NF-κB release and activation. (justia.com)
the activation of caspase-8, but rather improved activation of caspase-9 and advertised endonuclease G translocation into OHC nuclei. (colinsbraincancer.com)
Dietary sugars trigger the production of insulin or insulin-like peptides which are sensed by Insulin receptors at the cell surface and initiate a kinase cascade, leading to the activation of the downstream kinase Akt (PKB), the translocation of GLUT4 glucose transporters to the cell surface, and ultimately glucose uptake. (biomedcentral.com)
CDK42
The expression and translocation of cyclinD1-CDK4 in post-mitotic neurons indicate that they may have supplementary functions in differentiated neurons that might be associated with neuronal plasticity. (biomedcentral.com)
However, long-term depression (LTD) induced either by group I metabotropic glutamate receptors (mGluRs) agonist or by paired-pulse low-frequency stimulation (PP-LFS) was impaired in CDK4 inhibitor pretreated slices both from neonatal and adolescent animals. (biomedcentral.com)
Pathways1
Involvement of vesicular trafficking pathways in vRNP translocation To gain a detailed insight into nucleocapsid transport, we look overd vRNP localization in infected cells close to ultrastructural dissection using electron microscopy (EM). (upb.ro)
Depletion1
Depletion of UHRF1 rapidly induced genome-wide DNA demethylation in CRC cells. (biomedcentral.com)
Gene5
Burkitt lymphoma, or small noncleaved cell lymphoma, is a highly aggressive B-cell non-Hodgkin lymphoma (NHL) characterized by the translocation and deregulation of the c-myc gene on chromosome 8. (medscape.com)
Recent studies have demonstrated the existence of multiple copies of E2F1 gene in melanoma specimens which could explain the deregulated E2F1 activity in this type of cancer. (biomedcentral.com)
This is the first study that shows a relation between germline E2F1 CNV and melanoma, suggesting that altered copies of this gene might be a predisposing factor to skin cancer. (biomedcentral.com)
This strongly supports the amplification of E2F1 gene in predisposing to cancer. (biomedcentral.com)
The BIC gene is activated by promoter insertion at a retroviral integration site on chromosome 21q21 in B cell lymphomas induced by avian leukosis virus. (biomedcentral.com)
Potential therapeutic target1
Therefore, E2F1 has been considered as a potential therapeutic target for this form of skin cancer. (biomedcentral.com)
Protein1
DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1. (ncl.edu.tw)
Inflammation1
The molecular mechanisms underlying HIV-induced inflammation, which persists even during effective long-term treatment, remain incompletely defined. (biomedcentral.com)
Melanoma5
Since germline copy number variations (CNVs) have been associated with increased susceptibility to different types of cancer, the aim of our study was to assess germline E2F1 CNV in melanoma patients. (biomedcentral.com)
E2F1 CNV was measured in genomic DNA isolated from blood of 552 patients diagnosed with melanoma and 520 healthy subjects using TaqMan Copy Number Assays. (biomedcentral.com)
We found that patients diagnosed with melanoma (1.6%, 9/552) harboured frequently altered germline E2F1 copies compared to healthy subjects (0%, 0/520). (biomedcentral.com)
Therefore, subjects with multiple constitutive copies of E2F1 are at greater risk of developing melanoma when exposed to heat. (biomedcentral.com)
Given the pivotal role that E2F1 has in cell proliferation, its involvement in melanoma growth and progression is not surprising. (biomedcentral.com)
Immune1
Interestingly, circRNAs can also induce antiviral immune responses. (ijbs.com)
Chronic1
Author Summary The t(9;22) is a reciprocal translocation, which causes chronic myeloid leukemia (CML) and a subset of high risk acute lymphatic leukemia (ALL). (bios-mep.info)
Cells4
The increased calcium influx into the pancreatic beta cells induces insulin secretion, thereby lowering blood glucose levels. (medindex.am)
For the other hand, down regulation of Id proteins in epithelial cells is because of activated SMAD3 that induces activating transcription factor expression then together with ATF directly represses the Id promoter. (mirnaarray.com)
Eventually, ret inal IR damage to rats induced a reduction of vascular cells occurring seven to 14 days following reperfusion. (parp1inhibitor.com)
Interestingly, targeting WDR5 by siRNA and OICR-9429 could block IFN-γ-induced PD-L1 expression in PCa cells. (thno.org)
Exposure2
MiRNA-9 levels were synergistically increased following IFNγ/LPS co-exposure in isolated macrophages and in vivo, in an IFN-γ/LPS-induced mouse model of steroid-resistant airway hyperresponsiveness (AHR) [ 2 ] . (encyclopedia.pub)
Furthermore, we found that heat exposure alone can significantly induce E2F1 expression. (biomedcentral.com)
Inhibitor1
TGF B induces the expression of the CDK inhibitor p15INK4B inside a assortment of cell sorts. (mirnaarray.com)
Expression3
Considering that heat stress has been hypothesised as a contributing factor to skin cancer, we also investigated the effect of heat stress on E2F1 expression. (biomedcentral.com)
Moreover, TGF B can induce expression of p21CIP1 in various cell styles. (mirnaarray.com)
Co-expression of p96abolished the capacity of p185to induce a CML-like disease and led to the induction of ALL. (bios-mep.info)
Damage3
Nevertheless, tumors depend on residual DNA repair abilities to survive the DNA damage induced by genotoxic stress. (bmbreports.org)
Introduction The intraocular stress induced retinal ischemia reperfusion model is actually a handy device for studying the neuronal response to a transient ischemic damage. (parp1inhibitor.com)
Mino inhibits retinal neurodegeneration in quite a few designs of retinopathies, including light induced injury, axotomy, experimental glaucoma, photoreceptor degeneration, dia betic retinopathy, and IR damage. (parp1inhibitor.com)
Cell death1
Acetylation of p53 by KAT5 induces this cell death. (wikipedia.org)
Found1
Through the use of this procedure, a techni que induced variability of 32% for the measure was found. (dubinhibitors.com)
Regional2
Sound+ZVAD: treatment with ZVAD via regional delivery will not alter noise-induced p-AMPKlevels. (colinsbraincancer.com)
Sound+Nec-1: treatment with Nec-1 via regional delivery attenuates the noise-induced elevation of p-AMPKfluorescence. (colinsbraincancer.com)
Treatment1
Finally, auditory brainstem response practical measurements and morphological evaluation of OHCs demonstrated that ZVAD treatment decreases noise-induced deficits. (colinsbraincancer.com)