• RSV inhibited the expression of senescence markers (p53, p16, and p19), inflammasome markers (NLRP3 and Cas1 p20), and nuclear translocation of NF- κ B, hence alleviating infarction area, fibrosis, and cell apoptosis. (hindawi.com)
  • WWP2 interacts with transcription factor IRF7, promoting its non-degradative mono-ubiquitination, nuclear translocation and transcriptional activity, leading to upregulation of Ccl5 at transcriptional level. (nature.com)
  • LncARSR was observed to bind to YAP1, which inhibited phosphorylation nuclear translocation. (biomedcentral.com)
  • A1.R-induced PP2AC translocation to the particulate fraction was abrogated by adenoviral expression of the alpha subunit (Gαt1) coupled to the transducin G-protein coupled receptor. (kingston.ac.uk)
  • These data suggest that in ARVM, A1.R-induced PP2AC translocation to the particulate fraction occurs through a GiPCR-Gβγ-PI3K mediated intracellular signalling pathway, which may involve elevated PP2AC carboxylmethylation at leu309. (kingston.ac.uk)
  • In addition, neonatal rat cardiomyocytes (NRCMs), cardiac fibroblasts (CFs), and macrophages were isolated for in vitro experiments. (hindawi.com)
  • Ischemia/reperfusion (I/R) in mouse hearts and hypoxia/oxidative stress in neonatal rat cardiomyocytes have been associated with a downregulation of MG53. (biomedcentral.com)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
  • The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
  • Direct cardiac reprogramming of fibroblasts is an attractive therapeutic strategy for treating cardiovascular diseases because the adult heart has a low regeneration capacity [ 1 ]. (thno.org)
  • Single-cell reconstruction and mutation enrichment analysis identifies dysregulated cardiomyocyte and endothelial cells in congenital heart disease. (nih.gov)
  • Mechanisms by which Dox promotes cardiac damage and cardiomyocyte cell death have been investigated extensively, but a definitive picture has yet to emerge. (oncotarget.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • Longman, Michael R. , Ranieri, Antonella , Avkiran, Metin and Snabaitis, Andrew K. (2014) Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory Class G-Protein coupled receptors in cardiomyocytes. (kingston.ac.uk)
  • Previous studies have shown that the stimulation of adenosine type 1 receptors (A1.Rs) induces PP2AC carboxylmethylation and altered subcellular distribution in adult rat ventricular myocytes (ARVM). (kingston.ac.uk)
  • Background:Beta-2 adrenergic receptors (β2ARs) but not beta-2 adrenergic receptors (β1ARs) form a functional complex with L-type Ca2+ channels (LTCCs) on the cardiomyocyte membrane. (imperial.ac.uk)
  • We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
  • various second messengers in different combinations can act on the C1 domain to direct subcellular translocation of PKCε. (wikipedia.org)
  • Conclusions Our study demonstrated the transcriptional profile alteration in peripheral blood of chronic hypoxia rat model. (cams.cn)
  • The aim of this study was to investigate whether resveratrol (RSV) could ameliorate ischemia- and hypoxia-associated cardiomyocyte apoptosis and injury via inhibiting senescence signaling and inflammasome activation. (hindawi.com)
  • However, accumulating evidence suggests that MG53 has a potentially protective role in heart tissue, including in ischemia/reperfusion injury of the heart, cardiomyocyte membrane injury repair, and atrial fibrosis. (biomedcentral.com)
  • Since cardiomyocytes are terminally differentiated cells with limited self-renewal capacity, and membrane rupture is a major cause of cardiomyocyte cell death following injury, membrane repair is a necessary process for preserving cardiomyocyte viability [ 6 ]. (biomedcentral.com)
  • Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
  • Analysis of peripheral nerve regeneration after transection on the model of rat caudal nerve using tubulization and mesenchymal stem cells. (sav.sk)
  • In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
  • Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)
  • Methods The present study used a chronic hypoxia rat model to simulate the hypoxic state of CCHD patients. (cams.cn)
  • Two groups of Sprague-Dawley rats ( n =6 per group) were either exposed to hypoxia (10% O 2 ) or normoxia (21% O 2 ) for 3 weeks. (cams.cn)
  • Cardiomyocytes require large numbers of healthy-functioning mitochondria to ensure sufficient ATP production for contractile function and cell survival. (oncotarget.com)
  • Kiorpes TC, Hoerr D, Ho W, Weaner LE, Inman MG, Tutwiler GF (2021) Identification of 2-tetradecylglycidyl coenzyme A as the active form of methyl 2-tetradecylglycidate (methyl palmoxirate) and its characterization as an irreversible, active site-directed inhibitor of carnitine palmitoyltransferase A in isolated rat liver mitochondria. (bioblast.at)
  • Adipocyte-specific FGF21, which is endocrine FGF subfamily, knockout mice exhibit the inhibition of white adipose tissue browning in adaptive thermogenesis, and central infusion of FGF21 in obese rats increases insulin-induced suppression of hepatic glucose production and gluconeogenic expression, which in turn increases energy expenditure and insulin sensitivity. (biomedcentral.com)
  • The Combination of Resveratrol and Quercetin Attenuates Metabolic Syndrome in Rats by Modifying the Serum Fatty Acid Composition and by Upregulating SIRT 1 and SIRT 2 Expression in White Adipose Tissue - Evid Based Complement Alternat Med. (qualitycounts.com)
  • Toxic effects of Dox include cardiomyocyte damage and apoptotic and necrotic cell death [ 5 ]. (oncotarget.com)
  • Dox-induced over-production of reactive oxygen and nitrogen species (ROS and RNS) such as superoxide, hydrogen peroxide, hydroxyl radical and peroxynitrite has been proposed to be the main cause of Dox-induced acute cardiomyocyte toxicity and cell death by damaging various molecular constituents and organelles [ 9 - 12 ]. (oncotarget.com)
  • Enriched monolayer precursor cell cultures from micro-dissected adult mouse dentate gyrus yield functional granule cell-like neurons. (mdc-berlin.de)
  • specifically, the C2 domain of PKCε at amino acids 14-21 (also known as εV1-2) binds (RACK/RACK2), and peptide inhibitors targeting εV1-2 inhibit PKCε translocation and function in cardiomyocytes, while peptide agonists augment translocation. (wikipedia.org)
  • The sensors were subsequently expressed in neonatal rat ventricular myocytes and acutely isolated adult guinea pig ventricular myocytes. (johnshopkins.edu)
  • Persistence of ErbB2 and ErbB4 expression in neonatal and adult ventricular myocytes. (nih.gov)
  • We now show using RNA interference that CIP4 expression in neonatal rat ventricular myocytes is required for the induction of non-mitotic, hypertrophic growth by the α-adrenergic agonist phenylephrine, the IL-6 cytokine leukemia inhibitor factor, and fetal bovine serum, as assayed using morphometry, immunocytochemistry for the hypertrophic marker atrial natriuretic factor and [ 3 H]leucine incorporation for de novo protein synthesis. (biomedcentral.com)
  • B . Neonatal rat ventricular myocytes were transfected with control or CIP4 siRNA and then stimulated with 10 μM PE, 1000 U/mL LIF, or 10% FBS for two days as indicated. (biomedcentral.com)
  • Rcan1-1L overexpression activated autophagy flux and promoted translocation of the specific mitophagy receptor Parkin into mitochondria from the cytosol, whereas inhibition of autophagy flux resulted in the accumulation of Parkin-loaded mitochondria. (molcells.org)
  • Shaikh S, Troncoso R, Mondaca-Ruff D, Parra V, Garcia L, Chiong M, Lavandero S. The STIM1 inhibitor ML9 disrupts basal autophagy in cardiomyocytes by decreasing lysosome content . (cmcmed.cl)
  • This study only utilized male rats to avoid the influence of the oestrus cycle on food intake which may affect the diet-induced model [ 6 ]. (biomedcentral.com)
  • Protective paracrine effect of mesenchymal stem cells on cardiomyocytes[J]. Journal of Zhejiang University Science B, 2009, 10(8): 619-624. (zju.edu.cn)
  • Objective: The aim of this study was to test the protective effect of mesenchymal stem cells (MSCs) on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. (zju.edu.cn)
  • 3. ERBB2 triggers mammalian heart regeneration by promoting cardiomyocyte dedifferentiation and proliferation. (nih.gov)
  • In the adult mammalian heart, NCX expression levels increase during heart failure and arrhythmias as an intrinsic mechanism of cardiac functional compensation, though with potentially negative side-effects [ 5 ]. (biomedcentral.com)
  • Although a large number of drugs and agents (including Ca 2+ -channel blockers, mannitol, acadesine, adenosine, Na + /H + -exchange inhibitors, and N-acetylcysteine (an antioxidant)) have been shown to protect against ischemia-reperfusion injury (IRI) in the kidney in vitro and in vivo (in either rat or mouse), all have failed in either large animal or human trials [ 5 - 8 ]. (hindawi.com)
  • MATERIALS AND METHODS: Male Sprague-Dawley rats were undergone cecal ligation and puncture (CLP) or sham laparotomies. (bvsalud.org)
  • Methods: MSCs from Sprague-Dawley (SD) rats were separated and cultured. (zju.edu.cn)
  • PMID- 9357896 OWN - NLM STAT- MEDLINE DA - 19971120 DCOM- 19971120 LR - 20061115 PUBM- Print IS - 0003-3022 (Print) VI - 87 IP - 4 DP - 1997 Oct TI - Bupivacaine inhibition of L-type calcium current in ventricular cardiomyocytes of hamster. (nih.gov)
  • Such cardiotoxicity might involve an inhibition of cardiac L-type Ca2+ current (ICa,L). This study was designed to define the mechanism of bupivacaine inhibition of ICa,L. METHODS: Cardiomyocytes were enzymatically dispersed from hamster ventricles. (nih.gov)
  • 12. Neuregulin-1 triggers GLUT4 translocation and enhances glucose uptake independently of insulin receptor substrate and ErbB3 in neonatal rat cardiomyocytes. (nih.gov)
  • Sodium-dependent glucose cotransporters (SGLTs) are responsible for tissular glucose translocation. (iscpcardio.org)
  • Also, there were a trend of uric acid and glucose normalization for HRP as compared to H-fed rats. (biomedcentral.com)
  • We observed an increase in GLUT1 under hypoxia in a glucose-dependent manner, which we found to be directly associated with the translocation and stabilization of GLUT1 in the cell membrane. (molvis.org)
  • Male Wistar-Albino rats (n=51) were divided into four groups: sham (n=6), O 3 only (n=15), MNU only (n=15), and MNU+O 3 (n=15). (go.jp)
  • Male adult Wistar rats were divided randomly into six groups. (researchgate.net)
  • Total 48 male Wistar rats were divided into 4 groups: corn-starch group (CS), corn-starch + red pitaya juice group (CRP), high-carbohydrate, high fat group (HCHF) and high-carbohydrate, high fat + red pitaya juice (HRP). (biomedcentral.com)
  • We raised the possibility that, in a mouse model of physiological aging, defects in electromechanical properties of cardiomyocytes are important determinants of the diastolic characteristics of the myocardium, independently from changes in structural composition of the muscle and collagen framework. (nature.com)
  • Although the claim is commonly made that age-associated physiological changes predispose older adults to develop heart failure with normal EF, the aetiology of diastolic heart failure is unknown. (nature.com)
  • In cardiomyocytes, the exchanger extrudes Ca 2+ from the cytosol thereby allowing relaxation to occur but it may also function in Ca 2+ influx mode under certain physiological conditions, thereby contributing to cardiomyocyte contraction [ 4 ]. (biomedcentral.com)
  • The upstroke of the action potential (AP) triggers Ca 2+ entry via L-type channels, which results in cytosolic Ca 2+ rise by the translocation of Ca 2+ from the sarcoplasmic reticulum (SR) to the cytoplasm, initiating cell contraction 7 . (nature.com)
  • We previously showed that mitochondrial oxidative stress in the kidneys of Zucker diabetic fatty rats is associated with a decreased intracellular NAD+/NADH ratio and NAD+-dependent deacetylase Sirt3 activity, and increased expression of the NAD+-degrading enzyme CD38. (bvsalud.org)
  • In addition, apigenin down-regulated CD38 expression, and increased the intracellular NAD+/NADH ratio and Sirt3-mediated mitochondrial antioxidative enzyme activity in the kidneys of diabetic rats. (bvsalud.org)
  • The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. (zju.edu.cn)
  • Conclusion: MSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner. (zju.edu.cn)
  • In humans, CaMKII-δ9 and CaMKII-δ3, the major cardiac splice variants, inversely regulate cardiomyocyte viability with the former pro-death and the latter pro-survival. (stanford.edu)
  • PG - 1035-41 AB - Cerebral blood flow (CBF) in newborn infants is often below levels necessary to sustain brain viability in adults. (nih.gov)
  • The mechanical activity of the myocardium is initiated by the electrical excitation of the tissue, which acts as a functional syncytium in propagating membrane depolarization in cardiomyocytes. (nature.com)
  • 4. Improving murine embryonic stem cell differentiation into cardiomyocytes with neuregulin-1: differential expression of microRNA. (nih.gov)
  • Developmental events leading to the formation of adult muscular system, which includes differentiation of the various types of muscle cell precursors, migration of myoblasts, activation of myogenesis and development of muscle anchorage. (lookformedical.com)
  • Anselme F, Loriot S, Henry J‐P, Dionnet F, Napoleoni J‐G, Thuillez C, Morin J‐P. Inhalation of diluted diesel engine emission impacts heart rate variability and arrhythmia occurrence in a rat model of chronic ischemic heart failure. (comprehensivephysiology.com)
  • 2. Neuregulin stimulation of cardiomyocyte regeneration in mice and human myocardium reveals a therapeutic window. (nih.gov)
  • 17. Chronic administration of calcitriol enhanced neuregulin-1/ErbB signaling in rat myocardium. (nih.gov)
  • Cardiomyocytes constitute nearly 90% of the myocardium and alterations in the mechanisms of cell excitation, force generation and kinetics of contraction may be critical in conditioning the abnormalities of the old heart. (nature.com)
  • 8. Controlled delivery of fibroblast growth factor-1 and neuregulin-1 from biodegradable microparticles promotes cardiac repair in a rat myocardial infarction model through activation of endogenous regeneration. (nih.gov)
  • The increase in myocardial stiffness has been ascribed to changes in the composition of the extracellular matrix and/or to intrinsic characteristics of cardiomyocytes 6 . (nature.com)
  • The expression of NCX in cardiomyocytes is elevated in neonates, suggesting a greater role for the exchanger in early developmental stages [ 6 ]. (biomedcentral.com)
  • Rats were divided into the sham, CLP group, CLP+ CD38 expression lentivirus (CLP+ CD38 LV), CLP+ CD38 interference lentivirus (CLP+ CD38 Ri), CLP+ negative control lentivirus (CLP+NC) and the CLP+8-Br-cADPR groups. (bvsalud.org)
  • The epicardium also exerts key functions during injury responses in the adult and contributes to cardiac repair. (mdpi.com)
  • 2024. Kinetics of glyphosate and aminomethylphosphonic acid sorption onto montmorillonite clays in soil and their translocation to genetically modified corn. (nih.gov)
  • Diastolic stiffness of the heart was reduced after supplementation of red pitaya juice in corn starch fed rats. (biomedcentral.com)
  • We investigated the possible protective effect of combined pumpkin seed and Zingiber officinale extracts on sperm characteristics, epididymal histology and biochemical parameters of CP-treated rats. (researchgate.net)
  • HN - 2008 BX - Child Abuse, Adult Survivors MH - Agouti Signaling Protein UI - D054366 MN - D12.644.276.49 MN - D12.776.467.49 MN - D23.529.49 MS - A secreted protein of approximately 131 amino acids (depending on species) that regulates the synthesis of eumelanin (brown/black) pigments in MELANOCYTES. (nih.gov)
  • CaMKII-delta9 is the most abundant CaMKII-delta splice variant in the human heart and acts as a central mediator of DNA damage and cell death in cardiomyocytes. (stanford.edu)
  • Western blot analysis was performed on whole cell extracts (30µg lysate) of A-431 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), PC-3 (Lane 5), L6 (Lane 6), RSC96 (Lane 7), tissue extracts (30µg lysate) of Mouse Lung (Lane 8), Rat Stomach (Lane 9), Mouse Pancreas (Lane 10) and Rat Brain (Lane 11). (thermofisher.com)
  • 7. The role of neuregulin/ErbB2/ErbB4 signaling in the heart with special focus on effects on cardiomyocyte proliferation. (nih.gov)
  • 11. Nerves Regulate Cardiomyocyte Proliferation and Heart Regeneration. (nih.gov)
  • 14. Neuregulin1/ErbB4 signaling induces cardiomyocyte proliferation and repair of heart injury. (nih.gov)
  • CaMKII-delta9 Induces Cardiomyocyte Death to Promote Cardiomyopathy and Heart Failure. (stanford.edu)
  • Apigenin significantly reduced renal injuries, including tubulointerstitial fibrosis, tubular cell damage, and pro-inflammatory gene expression in diabetic rats. (bvsalud.org)
  • Red pitaya has been shown to protect aorta from oxidative damage and improve lipid profiles in hypercholesterolemic rats probably due to phytochemicals content including phenolics and flavonoids. (biomedcentral.com)
  • specifically, the C2 domain of PKCε at amino acids 14-21 (also known as εV1-2) binds (RACK/RACK2), and peptide inhibitors targeting εV1-2 inhibit PKCε translocation and function in cardiomyocytes, while peptide agonists augment translocation. (wikipedia.org)
  • The aim of this study was to investigate the changes in cardiac stiffness, hepatic and renal function in high-carbohydrate, high-fat diet-induced obese rats following supplementation of red pitaya juice. (biomedcentral.com)
  • In still another study, combined treatment of AS101 with low doses of paclitaxel enhanced survival of B16 melanoma tumefaction bearing rats by up controlling Fas/Apo 1 expression. (inhibitorkit.com)
  • Both sensors were calibrated in HEK293 cells and their responses correlated well with NFAT translocation to the nucleus, validating the biological relevance of the sensor readout. (johnshopkins.edu)
  • Both sensors demonstrated robust responses in myocytes and revealed kinetic differences in calcineurin activation during changes in pacing rate for neonatal versus adult myocytes. (johnshopkins.edu)
  • Here we show that an increase in the late Na + current ( I NaL ) in aging cardiomyocytes prolongs the action potential (AP) and influences temporal kinetics of Ca 2+ cycling and contractility. (nature.com)
  • and (iii) FP extension is initiated at a milk-suckling stage under a nephrin tyrosine-phosphorylated condition, while it is arrested at an adult stage, associated with a loss of nephrin-based signaling. (go.jp)
  • Epididymal epithelium and fibromascular thickness were also improved in extract-treated rats compared to control or CP groups. (researchgate.net)
  • combined effect of ginger and pumpkin extractson rat testis after CP injection. (researchgate.net)