Translational protein factors. Medical search. Frequent questions

Translational regulation refers to the control of the levels of protein synthesized from its mRNA. (wikipedia.org)
The termination of translation requires coordination between release factor proteins, the mRNA sequence, and ribosomes. (wikipedia.org)
Alternative splicing enables an mRNA to synthesize differently sized protein variants (isoforms) from the same gene that may have different cellular functions or properties. (genetex.com)
And 3) What is the functional relevance of mRNA condensation to translational control? (nih.gov)
Similar corresponding luciferase mRNA levels indicate translational rather than transcriptional alterations. (uzh.ch)
Direct assessment of translational regulation by polysomal profile analysis of HeLaS3 cells showed that HIF-1alpha (and to a lower extent ARNT) mRNA was found mainly in the translationally active polyribosomal fractions under both normoxic and hypoxic conditions. (uzh.ch)
The biological system is complex with many regulatory features such as DNA, mRNA, proteins, metabolites, and epigenetic features such as DNA methylation and histone post-translational modifications (PTMs). (rsc.org)
Quantitative proteomics will be applied to identify the differentially mRNA-bound proteins after the exposure of human cultured cells to ionizing radiation. (europa.eu)
C1QBP promoted the mRNA and protein expression of hypoxanthine catabolic enzyme XDH. (medsci.org)
In particular, upstream open reading frames (uORFs) are segments of 5′UTR mRNA sequences that can initiate and terminate translation upstream of protein-coding start codons. (nature.com)

In comparison to transcriptional regulation, it results in much more immediate cellular adjustment through direct regulation of protein concentration. (wikipedia.org)
This two-pronged response involves changes at both the transcriptional level (in the cell nucleus) and the translational level (in the cytoplasm). (elifesciences.org)
Here we outline the current status of the viral evasion mechanism underlying the regulation of the cGAS-STING pathway, focusing on how post-transcriptional modifications, viral proteins, and non-coding RNAs involve innate immunity during viral infection, attempting to inspire new targets discovery and uncover potential clinical antiviral treatments. (frontiersin.org)
The models of most immune evasion during innate immune responses are conserved, including altering the post-transcriptional modifications (PTMs) of vital proteins to inactivate or degrade these components, eluding DNA sensing from PRRs, decreasing cyclic GMP-AMP (cGAMP) cellular abundance, and modifying metabolism approach in host cells ( Eaglesham and Kranzusch, 2020 ). (frontiersin.org)
The heterodimeric hypoxia-inducible factor-1 (HIF-1), consisting of the subunits HIF-1alpha and HIF-1beta/ARNT, is a master transcriptional regulator of oxygen homeostasis. (uzh.ch)
This is a manifold response consisting of transcriptional and translational mechanisms that allow the adaptation of plants to many unfavorable environments. (novapublishers.com)
Studies have shown that the Wnt/β-catenin signaling pathway controls cell fate and function through the transcriptional and post-translational regulation of omics networks. (frontiersin.org)
In this project, I will employ biochemical, cell, systems biology and computational approaches to study the relevance of post-transcriptional regulators such as RNA-binding proteins and microRNAs in DDR. (europa.eu)

These time-of-day-dependent variations appear to be mediated by clock-dependent regulation of O-GlcNAc transferase and O-GlcNAcase protein levels, glucose metabolism/uptake, and glutamine synthesis in an NAD-independent manner. (nih.gov)
Collectively, these data suggest that the cardiomyocyte circadian clock increases protein O-GlcNAcylation in the heart during the active/awake phase through coordinated regulation of the hexosamine biosynthetic pathway and that protein O-GlcNAcylation in turn influences the timing of the circadian clock. (nih.gov)
Proper regulation of protein synthesis is vital for ensuring that the correct levels of protein are available for the cell to cope with changing conditions. (medlineplus.gov)
However, translational regulation of HIF-1alpha has not been directly analyzed so far. (uzh.ch)
Regulation of proteins through post-translational modifications, including reversible phosphorylation and ubiquitylation. (dundee.ac.uk)
Unlike reversible disassembly, which requires ATP hydrolysis on V 1 to break protein-protein interactions, Oxr1p mediated disassembly of V-ATPase is ATP independent and therefore a novel mode of activity regulation. (upstate.edu)
The results and implementations of this project will significantly contribute to the understanding of the regulation of DDR, as well as have potential clinical and translational impact. (europa.eu)
The results of this study reveal for the first time the molecular mechanism of the regulation of osteogenic differentiation by YTHDF1 under hypoxia and suggest that YTHDF1, together with its downstream factor THBS1, may be critical targets to counteract osteogenic inhibition under hypoxic conditions, providing promising therapeutic strategy for the hypoxia -induced bone loss in peri-implantitis . (bvsalud.org)

The corresponding mechanisms are primarily targeted on the control of ribosome recruitment on the initiation codon, but can also involve modulation of peptide elongation, termination of protein synthesis, or ribosome biogenesis. (wikipedia.org)
Among these are disorders resulting from mutations in protein synthesis machinery, including the ribosome and translation factors. (umd.edu)
ISRIB acts downstream of the phosphorylation of eIF2 (eIF2(αP)) and upstream of the activation of ATF4 (green arrow) and the repression of bulk protein synthesis (red down arrow). (elifesciences.org)
Equally importantly, ISRIB also prevented the reduction in overall protein synthesis that is normally observed within cells when eIF2 phosphorylation is high following integrated stress response kinase activation. (elifesciences.org)
The eIF2B protein helps regulate overall protein production (synthesis) in the cell by interacting with another protein, eIF2. (medlineplus.gov)
The eIF2 protein is called an initiation factor because it is involved in starting (initiating) protein synthesis. (medlineplus.gov)
Under some conditions, eIF2B increases protein synthesis by helping to recycle molecules called GTP, which carry energy to the initiation factor. (medlineplus.gov)
Under other conditions, it slows protein synthesis by binding tightly to the initiation factor, which converts the eIF2B protein into an inactive form and prevents recycling of GTP. (medlineplus.gov)
Impairment of eIF2B function makes it more difficult for the body's cells to regulate protein synthesis and deal with changing conditions and stress. (medlineplus.gov)
In contrast, the association of mRNAs for beta-actin and ribosomal protein L28 with the polyribosomal fractions was substantially reduced under hypoxic conditions, suggesting decreased overall protein synthesis. (uzh.ch)
At the start codon, peptide synthesis initiates when the 40 s subunit acquires the 60 s subunit with other translation initiation factors. (nature.com)
The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis. (bvsalud.org)
During protein synthesis at the ribosome numerous factors act early on the nascent polypeptide chain. (lu.se)

In addition to the host regulatory mechanisms response to disease, the microbiome can make changes to the expression of the host features such as their genes, proteins, and/or PTMs. (rsc.org)
Post-translational modifications (PTMs) of proteins, including phosphorylation, acetylation, ubiquitination, and SUMOylation, can regulate the function of proteins, determine the active state and subcellular location of proteins, and dynamically interact with other proteins related to carcinogenesis and progression ( 17 - 20 ). (frontiersin.org)

Phosphorylation: Phosphorylation will increase the observed size of the target protein due to the negatively charged phosphate group impacting SDS binding, thus altering the protein's electrophoretic mobility. (genetex.com)

Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Complement component 1 Q subcomponent binding protein (C1QBP) plays a vital role in the progression and metabolism of cancer. (medsci.org)
To investigate whether oral protein supplements benefit bone metabolism in patients with recent hip fracture. (acpjournals.org)
As an example, in post-mortem brain of patients with Alzheimer's disease (AD), several studies have reported reduction of insulin, insulin-like growth factor 1 and insulin receptor and an increase in tau protein and glycogen-synthase kinase-3β compared to healthy controls suggesting an impairment of metabolism in the AD patient's brain. (lu.se)

Many proteins are synthesised and folded in the endoplasmic reticulum, and when this organelle is under stress (that is, when it is unable to cope with its workload), three sensors (called PERK, IRE1 and ATF6) send signals to the rest of the cell to perform two tasks: to coordinate various ways of reducing the expression of genes, and to increase the protein folding capacity of the cell to meet demand. (elifesciences.org)
Paradoxically, these stress-induced signalling events also enhance the translation of the messenger RNAs for some proteins, including a transcription factor called ATF4 that modulates the expression of various genes, to ameliorate the perceived stress. (elifesciences.org)
The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
Our results suggest translation disrupting mechanisms relating uORF variation to reduced protein expression, and demonstrate that translation at uORFs is genetically constrained in 50% of human genes. (nature.com)
Specific uORFs are known to control protein expression by tuning translation rates of downstream protein-coding sequences, and potential uORFs have been identified in ~50% of all human protein-coding genes 1 , 2 . (nature.com)

For instance, due to their genetic flexibility, viruses have developed various viral proteins and non-coding RNAs to interrupt several checkpoints of cGAS-STING. (frontiersin.org)
This will provide a global insight into the dynamics of RNAs and proteins during DDR. (europa.eu)

These data support a model in which Trm9-catalyzed tRNA modifications promote fidelity during the translation of specific transcripts, with decreased wobble base modification leading to translational infidelity, protein errors and activation of protein stress response pathways. (nih.gov)
Transforming Growth Factor β (TGFβ), Bone Morphogenetic Protein (BMP) and Wnt signalling pathways. (dundee.ac.uk)
Recent multi-omics studies have revealed the heterogeneity and complexity of tumor features such as their genetic mutations, transcriptome, proteins, and signaling pathways. (rsc.org)
The mechanistic basis of DDR has been classically thought to consist of an ""early"" post-translational response, which results in activation of several signalling pathways and transciption factors. (europa.eu)
Specifically, proteins involved in metabolic pathways and in mitochondrial functions are mainly downregulated in mice fed with obesogenic diets compared to SD. (lu.se)

We found that functional inhibition, genetic ablation, and inactivation by post-translational modification of OGG1. (lu.se)
We found that functional inhibition, genetic ablation, and inactivation by post-translational modification of OGG1 significantly augment IFN-λ expression in epithelial cells infected by human respiratory syncytial virus (RSV). (lu.se)
Patients with abnormal APCR ratios should have genetic testing to confirm factor V Leiden mutation status. (medscape.com)
Data Sources: PubMed, clinicaltrials.gov Study Selections: A systematic and focused literature search was conducted of PubMed from March 2021 to December 2021 using keywords to either PubMed or BioMed Explorer including IL-33/ST2, genetic polymorphisms, transcription, translation, post-translation modification, nuclear protein, allergy, asthma, and lung disease. (cdc.gov)
Albert Salehi's group The etiology of metabolic syndrome and type 2 diabetes is complex and involves the interaction between both genetic and environmental factors including nutritional state with consequent metabolic signals. (lu.se)

Correct codon-anticodon pairing promotes translational fidelity, with these interactions greatly facilitated by modified nucleosides found in tRNA. (nih.gov)
These mutations are shown to also cause translational fidelity loss and implicate eEF2-ribosome interactions in reading frame maintenance. (umd.edu)
Selective microfluidics-based ligand enrichment followed by sequencing, or SMiLE-seq, combines antibody arrays, mechanical trapping, and next-generation sequencing readouts to provide a new platform for characterizing DNA-protein interactions. (genomeweb.com)
That year, the two published a paper in Science describing a method to trap low-affinity DNA-protein interactions, specifically those of transcription factors. (genomeweb.com)
By physically restraining bound molecules against an antibody array, they capture both transient interactions, which yeast two-hybrid or tandem mass spectrometry methods don't, and weak interactions, which are usually obliterated by the wash in protein-DNA microarrays. (genomeweb.com)
For SMiLE-seq, what the button captures is interactions between GFP-labeled transcription factors and a library of oligonucleotides. (genomeweb.com)
The transcription factors bind to any number of DNA motifs in the library, those interactions are trapped, and then the readout matches up each motif with the transcription factor via the barcode. (genomeweb.com)
As the assembly was carried out in the absence of ATP, this suggests that formation of the holoenzyme from the individual subcomplexes is solely driven by protein-protein interactions at the interface. (upstate.edu)
The proposed project aims to identify the dynamics of RNA-protein interactions during DNA damage response (DDR) using high-throughput "omics" approaches. (europa.eu)
Our data provide the structural framework for interactions of co-translational factors at the ribosomal tunnel exit. (lu.se)
1991), none of the the structural details of the heterogeneous protein surface and experimental studies mentioned so far could provide un- takes into account a variety of short-range interactions, direct ambiguous information about oligomer structure. (lu.se)

Overall, this work highlights salient points about ribosomopathies and translationopathies, their molecular mechanisms, and the relevance of translational fidelity to human health. (umd.edu)

PERK is one of four protein kinases that phosphorylate eIF2 in mammals in response to different signals. (elifesciences.org)

Bacteria often can't support over-expression needed to get workable quantities of protein for an assay, and in vitro expression often can't synthesize a fully functional transcription factor. (genomeweb.com)
PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
Burkholderia bacteria have large, flexible, multi-replicon genomes, a large metabolic repertoire, various virulence factors, and inherent resistance to many antimicrobial drugs ( 2 , 3 ). (cdc.gov)

Increasing protein O-GlcNAcylation (through pharmacological inhibition of O-GlcNAcase) results in diminished Per2 protein levels, time-of-day-dependent induction of bmal1 gene expression, and phase advances in the suprachiasmatic nucleus clock. (nih.gov)
This gene encodes one of the mitochondrial translation elongation factors. (nih.gov)
The results were evaluated with Gene Ontology (GO) enrichment analysis to reveal patterns of protein expression at progression while on osimertinib treatment. (au.dk)
One of the great missing pieces in our understanding of gene expression is how transcription factors regulate and control it in an orchestrated fashion. (genomeweb.com)
The EIF2B5 gene provides instructions for making one of five parts of a protein called eIF2B, specifically the epsilon subunit of this protein. (medlineplus.gov)
eIF2B, a mediator of general and gene-specific translational control. (medlineplus.gov)
In this review, we discuss considerations of the study design for each data feature, the limitations in gene and protein abundance and their rate of expression, the current data integration methods, and microbiome influences on gene and protein expression. (rsc.org)
Using these variants, we map and validate gene-disease associations in two independent biobanks containing exome sequencing from 10,900 and 32,268 individuals, respectively, and elucidate their impact on protein expression in human cells. (nature.com)
APOE is also the strongest risk factor gene for Alzheimer's disease and related dementia, and an independent risk factor for Type 2 diabetes and cardiovascular disease. (eurekalert.org)

What is the cellular localization of the protein? (embl.org)
These can be grouped into three major classes - chaperones that assist in folding, enzymes that modify the nascent chain and targeting factors that assist in protein localization. (lu.se)

GO enrichment analysis demonstrated that most of the significant proteins were related to the immune system, specifically the adaptive immune response. (au.dk)
The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells. (qiagen.com)
After we receive the sequence-verified expression constructs, we will first perform small scale expression and purification tests in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
After harvesting the V1 virus, we perform small scale expression and purification tests, in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)

PERK works by phosphorylating a protein called eIF2 that is needed to start the translation of messenger RNA into strings of amino acids, which fold to form proteins. (elifesciences.org)

Funding JG is funded by an NIHR Clinical Lectureship and Cancer Research UK Translational Sample Collection Grant (CRCPSC-Nov22/100002). (bmj.com)
On this floor Cardiovascular researchers facilitate high-impact, collaborative research across a wide spectrum of basic, translational, and clinical research in areas such as heart failure, cardiac proteins, blood clotting, and more. (luc.edu)

Protein post-translational cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active/mature forms with decreased MW (e.g., pro-caspases to active caspases). (genetex.com)
Activated protein C (APC) is the enzymatically active form of protein C after proteolytic cleavage by thrombomodulin-bound thrombin. (medscape.com)
In the vast majority of cases, the patient with APCR carries the Leiden variant of factor V, in which the APC cleavage site on factor V is altered. (medscape.com)

GATA binding protein 3 (GATA3) is a zinc-finger pioneer transcription factor involved in diverse processes. (bmj.com)
Deplancke himself wondered whether this was a scalable technology, or whether it would only be useful to labs interested in transcription factor function. (genomeweb.com)
Isolation of an active transcription factor. (qiagen.com)
A biotinylated DNA oligo containing a specific transcription-factor binding sequence was immobilized on a streptavidin-coated 96-well plate. (qiagen.com)
NX1 extraction buffer (Blank) or 10 µg nucleic acid binding protein fraction was added, washed, and detected colorimetrically in an ELISA procedure using a transcription-factor specific antibody. (qiagen.com)
Specificity of binding was demonstrated by addition of a 10x excess of non-biotinylated oligo that was able to displace the transcription factor. (qiagen.com)

In support, we have used phenotypic, reporter and protein-based assays to demonstrate increased translational infidelity in trm9Δ Saccharomyces cerevisiae cells. (nih.gov)
Using quantitative tRNA modification analysis, we determined that trm9Δ cells are only deficient in 2 of 23 tRNA modifications, with those 2, 5-methoxycarbonylmethyluridine (mcm ( 5) U) and 5-methoxycarbonylmethyl-2-thiouridine (mcm ( 5) s ( 2) U), classified as key determinants of translational fidelity. (nih.gov)
Treating cells with glycosylation inhibitors (e.g., tunicamycin to block N-linked glycosylation or Benzyl-α-GalNAc (BαG) to block O-glycosylation) can be used to generate the unmodified target protein. (genetex.com)
For example, cells must synthesize protein much faster if they are multiplying than if they are in a resting state. (medlineplus.gov)
We are combining the rapid genome editing capability afforded by CRISPR/Cas9 with advanced knowledge of protein chemistry to engineer robust molecular tools capable of selectively targeting individual proteins for desired functional modulation in cells. (dundee.ac.uk)
The cryo-EM analysis also revealed a population of free V 1 bound to Oxidation Resistance 1 (Oxr1p), a poorly characterized protein that has been implicated in protecting cells from oxidative stress. (upstate.edu)
Novel tumour-specific RNA-protein interacting partners may serve as candidates to selectively induce cell death in tumour cells. (europa.eu)
A RNA-Binding Protein 1 Regulates Osteogenesis of MC3T3-E1 Cells under Hypoxia via Translational Control of Thrombospondin-1. (bvsalud.org)

Walter and colleagues-who are based at UCSF, McGill University and Genentech-set out to identify an inhibitor molecule that would block the PERK arm of the unfolded protein response. (elifesciences.org)
van der Voorn JP, van Kollenburg B, Bertrand G, Van Haren K, Scheper GC, Powers JM, van der Knaap MS. The unfolded protein response in vanishing white matter disease. (medlineplus.gov)
Recently published literature has provided evidence that the unfolded protein response (UPR) is involved in the development of retinal degeneration. (molvis.org)

The Qproteome Nuclear Protein Kit is intended for molecular biology applications. (qiagen.com)
A high-salt buffer allows dissociation of nuclear binding proteins (such as transcription factors) and their removal by diffusion from the nuclei. (qiagen.com)
The Qproteome Nuclear Protein Kit delivers a nucleic acid binding protein fraction suitable for a wide range of activity assays. (qiagen.com)
Poly(ADP-ribosyl)ation is therefore an immediate, covalent, but transient post-translational modifications of nuclear proteins, induced by DNA lesions. (imim.es)
transient post-translational modifications of nuclear proteins by poly(ADP-ribosyl)ation. (imim.es)
The aim of this project is to investigate the role of poly(ADP-ribosyl)ation of nuclear proteins, catalysed by PARP family members (specially PARP-1 and PARP-2), as a critical signaling pathway at nuclear level, in both innate and adquire immune response. (imim.es)

The genome lacks identifiable virulence factors and has no apparent increase in encoded antimicrobial drug resistance, few insertion sequences, and few pseudogenes, suggesting this outbreak was an opportunistic infection by an environmental strain not adapted to human pathogenicity. (cdc.gov)

In terms of predicting where in the genome the transcription factors bind, the models we're able to derive seem to be, in general, actually better than the other assays. (genomeweb.com)
That turned out to be really useful in order to express transcription factors that other assays have trouble working with. (genomeweb.com)

Generally, these initiation factors are expressed in equal proportion to ribosomes, however experiments using cold-shock conditions have shown to create stoichiometric imbalances between these translational machinery. (wikipedia.org)

Sumoylation: Sumoylation of the target protein can cause a ~15-17 kDa increase of the observed MW due to the covalently attached SUMO protein. (genetex.com)
SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin signaling pathway. (frontiersin.org)
SUMOylation of proteins is an important mechanism in cellular responses to environmental stress ( 21 , 22 ). (frontiersin.org)
Proteins associated with the Wnt/β-catenin pathway have been identified as SUMOylated substrates, and evidences suggested that the initiation and progression of cancers depended on the function of the SUMOylation ( 23 ). (frontiersin.org)

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
Why does the observed western blot band size (i.e., molecular weight) differ from the expected band size for some proteins? (genetex.com)
Typically, SDS-PAGE allows electrophoretic separation of proteins in a sample by molecular weight (MW), with larger proteins migrating more slowly than smaller proteins. (genetex.com)
Detection of translational noncrystallographic symmetry (TNCS) can be critical for success in crystallographic phasing, particularly when molecular-replacement models are poor or anomalous phasing information is weak. (iucr.org)
If the correct TNCS is detected then expected intensity factors for each reflection can be refined, so that the maximum-likelihood functions underlying molecular replacement and single-wavelength anomalous dispersion use appropriate structure-factor normalization and variance terms. (iucr.org)
The molecular chaperone glucose-regulated protein 78 ( GRP78 ) or binding immunoglobulin protein (BiP), in addition to Ca 2+ binding and protein processing functions, possesses one more key role: master initiator of early UPR signaling. (molvis.org)

Bases downstream the stop codon affect the activity of these release factors. (wikipedia.org)
In fact, some bases proximal to the stop codon suppress the efficiency of translation termination by reducing the enzymatic activity of the release factors. (wikipedia.org)
The 5'UTR variant is shown to result in haploinsufficiency and p53 activation, while the missense variant impairs translational fidelity because of defective stop codon recognition. (umd.edu)

Oxr1p belongs to the group of TLDc domain containing proteins that are highly conserved in higher eukaryotes. (upstate.edu)

Instead, GH levels are usually checked in tests that measure other substances, such as hormones and proteins, that are related to GH production. (medlineplus.gov)

In yeast, the canonical Hsp70 protein Ssb acts together with the ribosome associated complex (RAC), which consists of the inactive Hsp70 protein Ssz and the Hsp40 protein Zuotin. (lu.se)
Structure determination of Ssb and RAC together with ribosome binding studies provide detailed insights into the interplay of this chaperone system, which evolved to link translation and protein folding. (lu.se)

We hypothesized that wobble uridine modifications catalyzed by tRNA methyltransferase 9 (Trm9) are essential for translational fidelity. (nih.gov)
These mutants exhibited translational fidelity defects and were demonstrated to have lower catalytic activity in vitro. (umd.edu)

Two representative features of the cellular stress response, which is universally conserved across eukarya and occurs in response to a variety of different noxious environmental conditions, are 1) the upregulation of the cytoprotective heat shock proteins and 2) biomolecular condensation of RNA and protein into assemblies. (nih.gov)
We aim to understand how the DUF1669 domain controls the function of the FAM83 family of proteins in their potentially diverse cellular roles. (dundee.ac.uk)

We show that uORF variants introducing new stop codons, or strengthening existing stop codons, are under strong negative selection comparable to protein-coding missense variants. (nature.com)

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG. (thermofisher.com)
The hallmarks of neurodegeneration include oxidative stress, proteasome impairment, mitochondrial dysfunction and accumulation of abnormal protein aggregates as well as metabolic alterations. (lu.se)

These same biological processes are also directly influenced by modification of proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc). (nih.gov)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

Activated transcription factors in conjunction with chromatin remodelers induce epigenetic changes that reprogram IFN responses. (lu.se)

Mechanistic studies (n = 39) including transcript variants and post-translational modifications related to the immunobiology of IL-33. (cdc.gov)

The paper, " Discovery of Nonlipogenic ABCA1 Inducing Compounds with Potential in Alzheimer's Disease and Type 2 Diabetes, " was published in the journal ACS Pharmacology and Translational Science . (eurekalert.org)

The results showed in this study suggest that, together with cytoskeletal proteins, mitochondria and metabolic proteins are changing their post-translational status in brains of obese mice. (lu.se)

Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. (nih.gov)

Low abundance proteins, on the other hand, produce weak spectra and often represent the interesting unknown processes. (lu.se)

Accumulation of unfolded proteins in the ER lumen triggers the dissociation of GRP78 from its quiescent UPR mediators. (molvis.org)
Thus, efficient translation of mouse HIF-1alpha in a situation where the general translation efficiency is reduced represents a prerequisite for the very rapid accumulation of HIF-1alpha protein upon exposure to hypoxia. (uzh.ch)

Most translation is shut down, while proteins involved in the stress response are efficiently produced. (nih.gov)
shown in red), a poorly characterized protein implicated in oxidative stress response. (upstate.edu)

This dissociation from PERK, ATF6, and IRE1 satisfies the demand for appropriate protein folding. (molvis.org)

These findings suggest that the ROS-generated epigenetic mark 8-oxoGua via its reader OGG1 serves as a homeostatic thresholding factor in IFN-λ expression. (lu.se)

Full length human recombinant protein of human MTRF1L produced in HEK293T cell. (thermofisher.com)

Polymorphism in this particular sequence has both positive and negative effects on the efficiency of base-pairing and subsequent protein expression. (wikipedia.org)
Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)

The normal reference value for the activated protein C resistance (APCR) ratio is greater than 2.1. (medscape.com)
APC resistance (APCR) is a hypercoagulability disorder in which factor V cannot be inactivated by APC. (medscape.com)

Here, we report that C. elegans LIN-28 represses the expression of LIN-46, a downstream protein in the heterochronic pathway. (stanford.edu)
Thus, the spatial combination of uORFs and protein-coding start codons can produce different effects on downstream protein translation. (nature.com)

Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)

How translation is reprogrammed to favor heat shock protein production post-transcriptionally is poorly understood, but biomolecular condensation has been linked to translational control. (nih.gov)
PAWS1 is a member of the poorly characterised FAM83 family of proteins that are linked through the conserved DUF1669 domain of unknown function, which possesses a pseudo-Phospholipase D catalytic motif. (dundee.ac.uk)

Moving cholesterol to where it is needed in the body has positive effects on many physiological processes and can help clear misfolded proteins that accumulate in the brain. (eurekalert.org)

The ALS8 protein VAPB interacts with the ER-Golgi recycling protein YIF1A and regulates membrane delivery into dendrites. (nih.gov)
For example, alkaline vacuole/lysosome are deficient in autophagy, Golgi pH regulates its ability to glycosylate proteins and failure to maintain endosomal pH perturbs with its ability to recycle receptors to the Plasma membrane or, the trans-Golgi. (upstate.edu)

This is unsatisfying since strong spectra are often produced by high abundant proteins, which typically represent the bulk of the cell activity. (lu.se)

We report that total cardiac protein O-GlcNAc levels exhibit a diurnal variation in mouse hearts, peaking during the active/awake phase. (nih.gov)

In this case, two to three fold changes in expression of initiation factors coincide with increased favorability towards translation of specific cold-shock mRNAs. (wikipedia.org)
RESULTS: We found that the expression of 7 proteins were significantly altered at PD, compared to a sample taken at osimertinib response. (au.dk)
These conditions include but are not limited to artery or vein occlusion in diabetic retinopathy, hypoxic retina in retinopathy of prematurity, aging in age-related macular degeneration, expression of mutant proteins in inherited retinal degeneration, traumatic injury leading to retinal detachment, and light sensitivity in the case of light-induced retinal degeneration. (molvis.org)
Here, we tested the hypothesis that the DNA repair protein OGG1 recognizes 8-oxoguanine (8-oxoGua) in promoters modulating IFN expression. (lu.se)
C1QBP significantly increased ROS level, apoptosis, and the expression of apoptotic proteins such as cleaved caspase-3 and bax/bcl2 via regulating XDH. (medsci.org)
In case expression constructs and/or protocols are already present, you can immediately fill out our protein expression and purification request form and send the completed form back to us. (embl.org)
Does the protein require interaction partners for stability (→ co-expression)? (embl.org)
Once suitable conditions have been identified, we proceed to large scale expression (1-6 liter per construct) and protein purification using a variety of chromatographic techniques. (embl.org)
For protein expression we use either the Sf21 or the Hi5 insect cell lines. (embl.org)
The aim of the study was to identify proteins present in the brain that are changing their expression based on the diet given to the mice. (lu.se)

Chemicals and Drugs1

Phenomena and Processes2

Information Science2

MRNA10

Transcriptional8

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Bacteria3

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Abundant1

Cardiac1

Expression10