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  • PERK
  • The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). (acris-antibodies.com)
  • 300µg PERK over-expressing 293T cell lysate (Lanes 1 and 2), or 800µg wild type (Lanes 3 and 4), and PERK knock out (Lanes 5 and 6) MEF cell lysate were immunoprecipated with 15µl anti-PERK, followed by immunobloting with 1/1000 dilution of anti-PERK in 5% milk/TBST buffer. (acris-antibodies.com)
  • Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2 , 293T cells over-expressing Myc-PERK K618A. (acris-antibodies.com)
  • genes
  • Although mutations in three different genes favoring the overproduction of Aβ are known to cause early-onset familial AD (FAD) [ 1 , 2 ], the etiology of sporadic AD that accounts for the majority of AD cases remains unclear. (springer.com)
  • These motifs were shown to alter translational control of certain genes through alteration of the poly(A) tail. (jimmunol.org)
  • 14 15 In most genes, the mRNA levels are modulated by estrogen by 2 major mechanisms. (ahajournals.org)
  • For more than 30% of its genes, we observe a delay in peak transcript abundance in the polysomal fraction as compared to the steady-state mRNA fraction, suggestive of strong translational control. (biomedcentral.com)
  • inhibitor
  • Bortezomib is a potent and selective inhibitor of the 26S proteasome, 8 , 9 a multisubunit protein complex present in the nucleus and the cytoplasm of all eukaryotic cells 10 that is responsible for the degradation of ubiquitinated proteins. (bloodjournal.org)
  • enzyme
  • Using recombinant PEK produced in Escherichia coli or Sf-9 insect cells, we demonstrate that PEK is autophosphorylated on both serine and threonine residues and that the recombinant enzyme can specifically phosphorylate eIF-2α on serine-51. (asm.org)
  • facilitate
  • To exploit cellular proteins for replication and to overcome host factors with antiviral activity, HIV has evolved a set of regulatory and accessory proteins to shape an optimized environment for its replication and to facilitate evasion from the immune system. (frontiersin.org)
  • kinases
  • In mammals, the phosphorylation was shown to be carried out by eIF-2α kinases PKR and HRI. (asm.org)
  • In addition to a catalytic domain with sequence and structural features conserved among eIF-2α kinases, PEK contains a distinctive amino-terminal region 550 residues in length. (asm.org)
  • A family of protein kinases phosphorylate eIF-2α in response to different cellular stress conditions. (asm.org)
  • organisms
  • The ribosome, a complex and dynamic nucleoprotein machine, provides the platform for amino acid polymerization in all organisms [ 1 , 2 ]. (hindawi.com)
  • consistent
  • Such an origin is also consistent with the eukaryotic rooting implied by the presence of an insert within the elongation factor EF-1A that is found in all known eukaryotic and eocytic (crenarchaeal) EF-1A sequences, but lacking in all paralogous EF-G sequences [ 3 ]. (hindawi.com)
  • proteins
  • This can cause the accumulation of unfolded or misfolded proteins in the ER lumen - the condition referred to "ER stress" [ 2 - 7 ]. (pubmedcentralcanada.ca)
  • ribosomal
  • eIF-2 associates with initiator Met-tRNA and GTP and participates in the ribosomal selection of the start codon. (asm.org)
  • Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. (uniprot.org)