LipidsConformational transitionsNucleic acidsHighly conservedComplexesKinaseMolecularCytoplasmicRegulateExtracellularLocalizationEpithelialInduceTranscriptionalChromatinInvasivenessDeleteriousSignaling pathwayDifferentiationAmyloid fibrilsRolesMagneticNucleusGenesMoleculesEukaryotesCrystallographyDynamicsGene expressionSignalsInteractionsZinc FingersSpectroscopyReceptorsMediatesInductionTargetsHistonesScaffoldsProcessesInteractionBiological2023SequenceMechanismInducesStructural changesCellBindingPercolationBasalLipid
Lipids1
- Changes in the redox state of cells affect proteins, lipids, and nucleic acids in different ways. (hindawi.com)
Conformational transitions1
- The standard enthalpy DeltaH degrees and the standard entropy DeltaS degrees for the conformational transitions determined from the NMR spectra are as follows: DeltaH degrees = 37 kJ mol-1 and DeltaS degrees = 130 J mol-1 K-1 for wild-type Ran.GTP. (mpg.de)
Nucleic acids1
- Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (uams.edu)
Highly conserved2
- The 14-3-3 proteins are highly conserved in all eukaryotes. (elifesciences.org)
- Krüppel-like factors (KLFs) are highly conserved zinc-finger proteins that regulate cellular transcription machinery [ 1 , 2 ]. (medsci.org)
Complexes1
- Protein complexes, which are formed as a result of these interactions, consist of two or more components that associate along specific pathways - protein association pathways. (lu.se)
Kinase1
- We have previously shown that HMGA2-induced transcription requires phosphorylation of H2A.X at S139, which in turn is mediated by the protein kinase ataxia telangiectasia mutated (ATM) 10 . (nature.com)
Molecular8
- In addition, the Biomarker Discovery Lab works closely with the Translational Research Lab on potential molecular or tissue based biomarkers that appear to be clinically important to transition into a CLIA setting. (massgeneral.org)
- Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition. (nih.gov)
- At molecular level, these processes may be triggered by epithelial-mesenchymal transition (EMT) and necessitates specific alterations in cell metabolism. (biomedcentral.com)
- Moreover, a molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in Fe-S cluster biogenesis proteins. (uga.edu)
- Research on Fe-S cluster biosynthesis in the Johnson group currently involves investigating the molecular mechanisms of Fe-S cluster assembly on scaffold proteins and the subsequent intact cluster transfer to acceptor or carrier proteins. (uga.edu)
- Molecular mobility in keratin-rich materials monitored by nuclear magnetic resonance: A tool for the evaluation of structure-giving properties. (lu.se)
- Molecular dynamics simulations and solid-state nuclear magnetic resonance spectroscopy measurements of C-H bond order parameters and effective correlation times in a POPC-GM3 bilayer. (lu.se)
- Protein structures are invaluable for biochemical studies and for mechanical or molecular mechanics calculations have become the understanding of how proteins work. (lu.se)
Cytoplasmic2
- We used the colorectal cancer cell-line HCT116 and normal colorectal cell-line CCD-18co for immunofluorescence staining and nuclear/cytoplasmic separated western blotting. (researchsquare.com)
- The protein was present in cytoplasmic granules and punctate nuclear bodies. (edu.au)
Regulate2
- Recently, several chromatin-related proteins have been shown to regulate ESC pluripotency and/or differentiation, yet the role of the major heterochromatin proteins in pluripotency is unknown. (biomedcentral.com)
- Histone chaperones are proteins that regulate the interaction of histones with other proteins and DNA and also prevent the highly basic histones from forming inappropriate aggregates [ 2 , 3 ]. (biomedcentral.com)
Extracellular4
- We found that TWIST1 significantly increased SNB19 and T98G cell line invasion in orthotopic xenotransplants and increased expression of genes in functional categories associated with adhesion, extracellular matrix proteins, cell motility and locomotion, cell migration and actin cytoskeleton organization. (nih.gov)
- The transcription factor serum response factor (SRF), a phylogenetically conserved nuclear protein, mediates the rapid transcriptional response to extracellular stimuli, e.g. growth and differentiation signals. (wikigenes.org)
- Receives Zn 2+ from the secreted, extracellular zincophore protein, Pra1 for uptake of the metal. (tcdb.org)
- Epithelial-mesenchymal transition (EMT) is a key cellular process in which immotile epithelial cells transform into mesenchymal cells through cell polarity loss, cell-cell junction disassembly, and extracellular matrix (ECM) alteration. (bmbreports.org)
Localization4
- Phosphorylation-resistant site mutations in PIF7 result in increased nuclear localization and shade-induced gene expression, and consequently augment hypocotyl elongation. (elifesciences.org)
- Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
- These studies have revealed that 14-3-3 proteins can interact with the phosphorylated forms of their client proteins in response to certain signals, and that this binding finalizes the signaling event by enabling a change in the subcellular localization, protein stability or intrinsic enzymatic activity of the client, or by promoting an association between the client and other proteins. (elifesciences.org)
- GFP-fused PfNapS fusion protein targeting indicates cellular localization of PfNapS in the parasite nucleus. (biomedcentral.com)
Epithelial11
- FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
- We previously showed that the bHLH transcription factor TWIST1 which orchestrates carcinoma metastasis through an epithelial mesenchymal transition (EMT) is upregulated in GBM and promotes invasion of the SF767 GBM cell line in vitro. (nih.gov)
- Furthermore, we demonstrated the biological relevance of this mechanism of transcriptional initiation within the context of TGFB1 signaling and epithelial-mesenchymal transition (EMT). (nature.com)
- Current interest in this process, which is described as an epithelial-mesenchymal transition (EMT), stems from its developmental importance and its involvement in several adult pathologies. (rupress.org)
- The EMT International Association was formed in 2002 to provide an international body for those interested in EMT and the reverse process, mesenchymal-epithelial transition, and, most importantly, to bring together those working on EMT in development, cancer, fibrosis, and pathology. (rupress.org)
- Karyopherin-α3 (KPNA3), a karyopherin- α isoform, is intimately associated with metastatic progression via epithelial-mesenchymal transition (EMT). (bmbreports.org)
- This can promote epithelial-mesenchymal transition (EMT) of a tumor such as malignant glioma (4). (researchatlanta.org)
- We measured epithelial-mesenchymal transition (EMT) markers and migration capacity of HCT116 in the context of WNT7b knocked-down using short interfering RNA. (researchsquare.com)
- Epithelial-mesenchymal transition (EMT) is a developmental process by which epithelial cells are converted to mesenchymal cells during embryogenesis, tissue remodeling, wound healing, and tumor metastasis [4, 5]. (researchsquare.com)
- Its role is often interpreted in the context of the epithelial-mesenchymal transition. (iiarjournals.org)
- The experimental and physiological observations regarding the tumor-suppressor role of E-cadherin are often interpreted in the context of the epithelial-mesenchymal transition. (iiarjournals.org)
Induce2
- This heterodimer complex phosphorylates the intracellular proteins SMAD2 and SMAD3 activating a signaling cascade to induce several nuclear transduction proteins. (researchatlanta.org)
- Disruption of the thymic micro-environment resulted in loss of DNA-binding, suggesting that lymphocyte-stromal cell interactions induce and maintain these proteins in a DNA-binding form. (aai.org)
Transcriptional2
- Although HMG proteins do not possess intrinsic transcriptional activity, they are called architectural transcription factors because they modulate the transcription of their target genes by altering the chromatin structure at the promoter and/or enhancers 15 . (nature.com)
- Deleterious, protein-altering variants in the transcriptional coregulator ZMYM3 in 27 individuals with a neurodevelopmental delay phenotype. (uams.edu)
Chromatin4
- In addition to nucleosomes, chromatin contains non-histone chromatin-associated proteins, of which the high-mobility group proteins are the most abundant. (nature.com)
- These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
- Chromatin is highly dynamic, a characteristic that is vital in regulating nuclear processes such as transcription and replication which require access to DNA. (biomedcentral.com)
- In addition to playing an important role in histone exchange during nuclear processes, histone chaperones function in nucleocytoplasmic shuttling of histones, in histone storage, in nucleosome assembly and they act as a link between chromatin remodeling factors and histones [ 2 , 3 ]. (biomedcentral.com)
Invasiveness1
- E-cadherin is a well-known tumor suppressor protein and loss of its expression in tumor cells is associated with increased tumor invasiveness and metastasis [ 18 , 19 ]. (biomedcentral.com)
Deleterious1
- 2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. (upenn.edu)
Signaling pathway1
- With the induction of such proteins the TGFβ signaling pathway influences cellular proliferation differentiation motility survival and apoptosis in tumor cells. (researchatlanta.org)
Differentiation2
- Here we identify Heterochromatin Protein 1β (HP1β) as an essential protein for proper differentiation, and, unexpectedly, for the maintenance of pluripotency in ESCs. (biomedcentral.com)
- Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
Amyloid fibrils1
- Lipid dynamics and phase transition within α-synuclein amyloid fibrils. (lu.se)
Roles2
- Fe-S clusters are present in more than 300 different types of enzymes or proteins and play crucial roles in fundamental life processes such as respiration, photosynthesis and nitrogen fixation. (uga.edu)
- Ameiotic1 ( Am1 ) encodes a plant-specific nuclear protein (AM1) required for meiotic entry and progression through early prophase I. Pollen mother cells (PMCs) remain mitotic in most am1 mutants including am1-489 , while am1-praI permits meiotic entry but PMCs arrest at the leptotene/zygotene (L/Z) transition, defining the roles of AM1 protein in two distinct steps of meiosis. (biomedcentral.com)
Magnetic2
- Low-temperature magnetic circular dichroism studies of iron-sulfur proteins. (uga.edu)
- In particular, they are and nuclear magnetic resonance (NMR) refinement. (lu.se)
Nucleus1
- Proteins found in the nucleus of a cell. (uams.edu)
Genes3
- The clinical relevance of putative TWIST target genes SNAI2 and fibroblast activation protein alpha (FAP) identified in vitro was confirmed by their highly correlated expression with TWIST1 in 39 human tumors. (nih.gov)
- It has been demonstrated that the bolting and flowering signals from internal or external factors usually converge on a few integrator genes which serve as flowering inducers or repressors during floral transition. (frontiersin.org)
- Subtractive hybridization was used in an attempt to identify genes that were up-regulated during this erythroid to myeloid transition. (edu.au)
Molecules3
- View of a protein: Max Planck researchers have used the COLD method to visualize the four binding sites of biotin (labelled by red dye molecules) in the protein streptavidin, whose structure is already known. (mpg.de)
- We are therefore able to look into protein molecules. (mpg.de)
- HMGB1 is an abundant protein, 10 6 molecules per cell [ 7 ], which has been postulated as a redox sensor [ 8 ]. (hindawi.com)
Eukaryotes1
- Proteins containing CCCH Znf domains include Znf proteins from eukaryotes involved in cell cycle or growth phase-related regulation, e.g. human TIS11B (butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse TTP growth factor-inducible nuclear protein, which has the same function. (embl.de)
Crystallography2
- We also collaborate with other groups for Mossbauer, electron-nuclear double resonance (ENDOR), X-ray absorption and X-ray crystallography studies of proteins prepared in our laboratory. (uga.edu)
- The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)
Dynamics6
- Protein dynamics studied by NMR spin relaxation. (lu.se)
- Protein dynamics studied by NMR. (lu.se)
- Solid-liquid phase transitions of triglycerides in griebenschmalz, smalec, and fedt studied using 13C solid-state NMR with dynamics-based spectral filtering. (lu.se)
- Quasielastic incoherent neutron scattering (QENS) is a well-suited experimental method to study protein dynamics from the picosecond to several nanoseconds and in the Ångström length-scale. (lu.se)
- In my presentation, I will summarize recent QENS and NSE results on the dynamics of the intrinsically disordered myelin basic protein (MBP) and the chemically denatured bovine serum albumin (BSA) (1,2,3). (lu.se)
- For metal- powerful methods to obtain and compare reaction and activation loproteins, a third method to obtain local information about the energies for suggested enzyme mechanisms and they can also provide atomic details about the protein dynamics. (lu.se)
Gene expression1
- Because FK506 specifically inhibits the appearance of mature single-positive thymocytes, gene expression regulated by p68 c-rel may play a role in selection and maturational signals involved in the double-positive to single-positive transition. (aai.org)
Signals2
Interactions1
- Another protein containing this domain is the human splicing factor U2AF 35kDa subunit, which plays a critical role in both constitutive and enhancer-dependent splicing by mediating essential protein-protein interactions and protein-RNA interactions required for 3' splice site selection. (embl.de)
Zinc Fingers1
- Identification of four CCCH zinc finger proteins in Xenopus, including a novel vertebrate protein with four zinc fingers and severely restricted expression. (embl.de)
Spectroscopy2
- The interaction of Ran with the guanine nucleotide exchange factor protein RCC1 was also studied by (31)P NMR spectroscopy. (mpg.de)
- Neutron Spin-Echo spectroscopy (NSE) on the other hand, offers the highest energy resolution in the field of neutron spectroscopy and allows the study of slow collective motions in proteins up to several hundred nanoseconds and in the nanometer length-scale. (lu.se)
Receptors1
- 2022). Nuclear import receptors are recruited by FG-nucleoporins to rescue hallmarks of TDP-43 proteinopathy. (upenn.edu)
Mediates1
- Here we will focus on HMG AT-hook 2 protein (HMGA2), a member of the HMGA family that mediates transforming growth factor beta 1 (TGFB1, commonly known as TGFβ1) signaling 16 . (nature.com)
Induction1
Targets2
- Thus, it is important to understand the fundamental biological processes of P. falciparum , which may provide avenues for the identification of new protein targets for development of new anti-malarials. (biomedcentral.com)
- Iron-sulfur protein maturation in Helicobacter pylori:Identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets. (uga.edu)
Histones1
- Nucleosome assembly proteins (NAPs) are histone chaperones that are crucial for the shuttling and incorporation of histones into nucleosomes. (biomedcentral.com)
Scaffolds2
- They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
- Scientists, have tapped oil and water to create scaffolds of self-assembling, synthetic proteins called peptoid nanosheets. (materialstoday.com)
Processes2
- This review describes recent advances in the role of human HMGB proteins and other proteins interacting with them, in cancerous processes related to oxidative stress, with special reference to ovarian and prostate cancer. (hindawi.com)
- Sequestration in many of the processes identified can also result in the release of ox- of transition metals, principally iron above as necessary for conversion ygen radicals ( Hrycay and Bandiera, and copper, in their oxidized forms of normal cel s into cancer cel s. (who.int)
Interaction1
- Pra1 is a cell surface protein with a single N-terminal TMS involved in the host-parasite interaction during candidal infection. (tcdb.org)
20231
- 2023). A minimal construct of nuclear-import receptor Karyopherin-β2 defines the regions critical for chaperone and disaggregation activity. (upenn.edu)
Sequence2
- Extensive sequence and structural comparisons were done with the crystal structures available for NAP/SET family of proteins. (biomedcentral.com)
- We find that several proteins that bind the kappa B sequence in vitro are constitutively activated in freshly isolated thymocytes. (aai.org)
Mechanism1
- Researchers have studied colloidal solid-solid transitions and discovered a surprising mechanism that facilitates one of these routes. (materialstoday.com)
Induces2
- Here, we show that shade light induces the de-phosphorylation and nuclear accumulation of PIF7. (elifesciences.org)
- Here we show that high-mobility group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are required by the histone chaperone FACT complex to incorporate nucleosomes containing the histone variant H2A.X. Further, phosphorylation of H2A.X at S139 (γ-H2AX) is required for repair-mediated DNA demethylation and transcription activation. (nature.com)
Structural changes2
- The method thereby opens up the possibility of observing structural changes of proteins associated with specific diseases. (mpg.de)
- The protein HMGB1 behaves as a redox sensor and its structural changes, which are conditioned by the oxidative environment, are associated with different functions of the protein. (hindawi.com)
Cell1
- Cross-sectional associations between shiftwork and work hours with log-transformed high-sensitivity C reactive protein (CRP) and white blood cell (WBC) count were examined by multiple linear regression analysis, overall and by race-sex subgroups. (cdc.gov)
Binding3
- In complex with the Ran-binding protein RanBP1, one of the Ran.GTP conformations (state 2) is stabilized. (mpg.de)
- The binding site in Pra1 is in the C-terminal region of this 299 aa protein ( Łoboda and Rowińska-Żyrek 2017 ). (tcdb.org)
- Polyglutamine-binding protein 1 ( PQBP1 ) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. (xenbase.org)
Percolation1
- Simulation setup for "Percolation transition prescribes protein size-specific barrier to passive transport through the nuclear pore complex. (illinois.edu)
Basal1
- Sections were immunostained for estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her2), basal cytokeratin CK5, nuclear protein Ki67 and E-cadherin with a Leica Bond-Max autostainer. (iiarjournals.org)
Lipid1
- Ganglioside GM3 stimulates lipid-protein co-assembly in α-synuclein amyloid formation. (lu.se)