BACs are now being utilized to a greater extent in modelling genetic diseases, often alongside transgenic mice. (wikipedia.org)
Here, we tested this hypothesis using AR113Q knockin and human bacterial artificial chromosome/clone (BAC) transgenic mice that express the full-length polyQ-AR and display androgen-dependent weakness, muscle atrophy, and early death. (nih.gov)
Mutation of a self-processing site in caspase-8 compromises its apoptotic but not its nonapoptotic functions in bacterial artificial chromosome-transgenic mice. (southernbiotech.com)
Additional traditional technologies we offer to staff, faculty, and external researchers are the production of transgenic mice by pronuclear injection of plasmid DNA or bacterial artificial chromosome DNA and the production of ESC chimeras by blastocyst injection. (nyu.edu)
X. Trefoil peptide and EGF receptor/ligand transgenic mice. (embl.de)
To uncover the genetic basis for lower immobility time, we first performed quantitative trait locus (QTL) mapping using CS and C57BL/6J mice, which revealed significant QTLs on chromosomes (Chrs) 4 (FST) and 5 (TST and FST). (fujita-hu.ac.jp)
All these phenotypes were rescued in transgenic mice with bacterial artificial chromosomes containing wild-type Usp46. (fujita-hu.ac.jp)
Modification of bacterial artificial chromosomes (BACs) and preparation of intact BAC DNA for generation of transgenic mice. (cornell.edu)
Given the large size of BACs, most if not all regulatory sequences of a gene are present and can be used to direct faithful and tissue-specific expression of heterologous genes in vitro in cell cultures and in vivo in BAC-transgenic mice. (hal.science)
We describe here an optimized and comprehensive protocol to select, modify, and purify BACs in order to generate BAC-transgenic mice. (hal.science)
Next, I produced transgenic mice carrying cre driven by mouse Ins1 using a bacterial artificial chromosome （BAC）construct. (docksci.com)
We have created lines of mice that express human wild-type (hWT) or G2019S Lrrk2 via bacterial artificial chromosome (BAC) transgenesis. (elsevierpure.com)
In vivo analysis of the dopaminergic system revealed abnormal dopamine neurotransmission in both hWT and G2019S transgenic mice evidenced by a decrease in extra-cellular dopamine levels, which was detected without pharmacological manipulation. (elsevierpure.com)
Food-associated estrogenic compounds induce estrogen receptor-mediated luciferase gene expression in transgenic male mice. (knaw.nl)
APOL1 renal risk variants promote cholesterol accumulation in tissues and cultured macrophages from APOL1 transgenic mice. (omicsdi.org)
Tissues and bone marrow (BM)-derived monocytes were isolated from wild-type mice (WT) and from BAC/APOL1 transgenic (APOL1-G0, APOL1-G1, and APOL1-G2) mice, which carry a bacterial artificial chromosome that contains the human APOL1 genomic region. (omicsdi.org)
We developed coisogenic bacterial artificial chromosome (BAC) transgenic mice harboring either the wild-type (G0), G1 or G2 forms of human APOL1. (omicsdi.org)
Cis effect of lacZ sequences in transgenic mice. (shengsci.com)
Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. (shengsci.com)
Skeletal defects in Osterix-Cre transgenic mice. (shengsci.com)
To handle this presssing concern, we produced transgenic mice that portrayed the individual gene. (cancer-ecosystem.com)
Outcomes Era of transgenic mice that Aceneuramic acid hydrate exhibit the individual gene We reasoned a transgene formulated with the entire individual gene plus lengthy flanking sequences might confer tissue-specific, basal, and inducible appearance of individual P-selectin in mice as the indigenous gene will in human beings. (cancer-ecosystem.com)
Arrows tag places of three pairs of PCR primers utilized to judge incorporation from the gene into DNA of transgenic mice. (cancer-ecosystem.com)
Total RNA was extracted from lungs of three transgenic lines (series, denoted as mice simply. (cancer-ecosystem.com)
Knockout and transgenic mice have been developed for specific T-cell disorders and are recognized to have helped predict human genetic disorders. (medscape.com)
To study the dynamic relationship of CD28-CD80 and TCR interactions in the T cell-DC IS during Ag-specific T cell activation, we generated CD80-eCFP mice using bacterial artificial chromosome transgenic technology. (ox.ac.uk)
The distribution of putative AT(1A)R-expressing cells was mapped throughout the brains of three transgenic mice with a bacterial artificial chromosome-expressing green fluorescent protein under the control of the AT(1A)R promoter. (edu.au)
Artificial chromosome-transgenic3
To this end, we generated a novel multicistronic BAC (bacterial artificial chromosome) transgenic mouse line under the regulatory elements of the Npas1 gene. (nih.gov)
We developed a conditional Bacterial Artificial Chromosome transgenic mouse (BACHD) that contains the entire human huntingtin genomic locus modified to include the Huntington's disease (HD) causing mutation. (nih.gov)
Results: Using novel bacterial artificial chromosome transgenic nkx6.1 and ascl1b reporter lines, we established that nkx6.1-positive cells give rise to all the pancreatic cell types and ascl1b-positive cells give rise to all the endocrine cell types in the zebrafish embryo. (refine.bio)
BACs3
BACs can also be utilized to detect genes or large sequences of interest and then used to map them onto the human chromosome using BAC arrays. (wikipedia.org)
Bacterial Artificial Chromosomes (BACs) are ultra-low copy vectors that can hold up to 300 kb of genomic fragments, making them ideal vectors for the introduction of entire genes including the regulatory regions into the rat genome. (appliedstemcell.com)
Most genome projects have relied on the sequencing of bacterial artificial chromosomes (BACs), which encompass 100-300 kb of genomic DNA. (hal.science)
Genes2
F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. (wikipedia.org)
GM corn and cotton contain bacterial genes that kill insects and confer herbicide-resistance on the crops. (jrank.org)
Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis. (cornell.edu)
Zebrafish2
Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis. (shengsci.com)
To identify the pancreatic cells able to give rise to new beta cells in zebrafish, we generated new transgenic lines allowing the tracing of multipotent pancreatic progenitors and endocrine precursors. (refine.bio)
Clone3
We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. (shengsci.com)
We isolated a bacterial artificial chromosome clone composed of all 17 exons and 16 introns from the individual gene (Johnston et al. (cancer-ecosystem.com)
A) Schematic of bacterial artificial chromosome clone formulated with all 17 exons and 16 introns from the individual gene plus huge 5 and 3 flanking sequences. (cancer-ecosystem.com)
Recombination2
To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin (Adipoq) gene promoters, as well as a tamoxifen-inducible Cre driven by the aP2 gene promoter (iaP2), were bred to the Rosa26R (R26R) reporter. (diabetesjournals.org)
In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. (hal.science)
Genome2
BAC transgenic rats are similar to other knock-in models in the expression pattern of the gene of interest, except that the BAC is randomly integrated into the genome as a transgene. (appliedstemcell.com)
Antony joined The University of Manchester as Senior Experimental Officer at the Transgenic Unit core facility in 2014, and has been Manager at the rebranded Genome Editing Unit since 2018. (bitesizebio.com)
Pronuclear1
Alternatively, you can choose to have us do the BAC modification strategy and cloning , and you can do the pronuclear injections and transgenic rat screening yourself. (appliedstemcell.com)
Green fluores1
Production of green fluorescent protein transgenic embryonic stem cells using the GENSAT bacterial artificial chromosome library. (cornell.edu)
Lrrk21
Therefore, the authors concluded that LRRK2 inhibition may not represent a suitable therapeutic strategy for the treatment of PD.54 Another research group created inducible transgenic rats expressing LRRK2 with G2019S substitution and recapitulated the initiation process of dopaminergic dysfunction. (campaignfornonviolentschools.org)
Antigens1
Inadequate antibody responses directed against bacterial polysaccharide antigens cause an increased risk for sinopulmonary infections caused by encapsulated organisms. (medscape.com)
Knockin1
However, the mutation was not sufficient to develop dopaminergic neurodegeneration or to induce neuron death in transgenic rats.57 Data obtained from a R1441C knockin mouse suggested that this mutation impairs stimulated dopamine neurotransmission and D2 receptor function. (campaignfornonviolentschools.org)
Inactivation1
For example, XIST (X-inactive specific transcript), one of the first described lncRNAs has a low level of sequence conservation, but a highly conserved function across placental mammals - inactivation of the X chromosome [ 5 ]. (biomedcentral.com)
Phenotypes1
However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi. (shengsci.com)
Rats3
The results shown below are PCR screening data for F1 transgenic rats (Figure 1) that were confirmed by sequencing to have the gene of interest inserted at desired genomic locus. (appliedstemcell.com)
Twenty-two (22) F1 germline transmitted transgenic rats were identified using three sets of PCR primers (Lanes 1, 2, and 3) to identify transgene insertion at desired locus. (appliedstemcell.com)
Disease3
Future applications of transgenic technology to these areas are likely to be productive in furthering our understanding of the biology of these peptides in health and disease. (embl.de)
Recessive, gain-of-function toxicity in an APOL1 BAC transgenic mouse model mirrors human APOL1 kidney disease. (omicsdi.org)
Whereas host genetic susceptibility plays a key role in modulating disease manifestation, variations in bacterial virulence properties contribute to infection severity. (cdc.gov)
Generate4
Plant cells that have been transformed by the plasmid can be selected on media containing the antibiotic, and then cultured to generate new, transgenic plants. (jrank.org)
To generate a transgenic rat model with a ChR2-GFP insertion (10.5 kb) at a neuronal gene of interest using BAC technology. (appliedstemcell.com)
The purpose of this project is to generate a transgenic rat model with GFP insertion at a specific locus. (appliedstemcell.com)
To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). (hal.science)
Human2
Cosmid End-sequence profiling Fosmid Human artificial chromosome Secondary chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W (June 1989). (wikipedia.org)
The consumption of milk from transgenic goats producing human lysozyme (hLZ) in their milk therefore has the potential to positively impact intestinal health. (shengsci.com)
Vectors1
Furthermore, based on these technics, transgenic marmosets using lentiviral vectors were produced. (docksci.com)
Cells1
The present invention relates to methods and materials used to express HBM protein in animal and transgenic animal cells. (zhangqiaokeyan.com)
Gene expression1
He then pursued post-doctoral research at the University of Liverpool, developing recombinant Bacterial Artificial Chromosomes for the single cell microscopy of NF-kappaB translocation dynamics, and the impact on gene expression, in both cell lines and transgenic animal models. (bitesizebio.com)
Wild-type2
In this review, we provide an up-to-date overview of the current knowledge of how A. rhizogenes induces root formation, on the new uses for A. rhizogenes in tissue culture and composite plant production (wild-type shoots with transgenic roots), and the recent development of a disarmed version of A. rhizogenes for stable transgenic plant production. (bioone.org)
The present invention also relates to transgenic animals expressing the high bone mass gene, the corresponding wild type gene and mutants thereof. (zhangqiaokeyan.com)
Cell5
Using a combinatorial transgenic and immunohistochemical approach, we discovered that parvalbumin-expressing neurons and Npas1-expressing neurons in the GPe represent two nonoverlapping cell classes, amounting to 55% and 27% of the total GPe neuron population, respectively. (nih.gov)
Hairy roots are induced by the incorporation of a bacterial-derived segment of DNA transferred (T-DNA) into the chromosome of the plant cell. (bioone.org)
Chronic pain elicits synaptic modifications in nucleus accumbens To determine whether chronic pain elicits synaptic changes in NAc circuitry we prepared brain slices from bacterial artificial chromosome (BAC) transgenic animals and made targeted whole-cell recordings from visually identified medium spiny neurons (MSNs) belonging to either the direct pathway D1 dopamine receptor expressing MSNs (D1-MSNs) or the indirect pathway D2 dopamine receptor expressing MSNs (D2-MSNs) (18). (pkc-inhibitor.com)
Thus, the aim of this work is to carry out a review of the literature on the role of bacterial endotoxin in the etiology of periapical lesions, its mechanism of action, and to elucidate molecular mechanisms involved in endotoxin's recognition by the immune system and cell activation. (bvsalud.org)
Therefore, the objective of this study is to carry out a review of the literature on the role of bacterial endotoxin in the etiology of periapical lesions, as well as to elucidate molecular mechanisms involved in its recognition by the immune system and in cell activation. (bvsalud.org)
Applications1
The development and applications of the bacterial artificial chromosome cloning system" (PDF). (wikipedia.org)
Death1
This information is important because endotoxin is released during multiplication or bacterial death, causing a series of biological effects that lead to an inflammatory reaction and resorption of mineralized tissues. (bvsalud.org)
Effect2
In order to investigate the effect of hLZ-containing milk on the inflammatory response, young pigs were fed pasteurized milk from hLZ or non-transgenic control goats and quantitative real-time PCR was performed to assess local expression of TNF-α, IL-8, and TGF-β1 in the small intestine. (shengsci.com)
Chromosome spreads reveal that Rec8-N expression has no effect in primary spermatocytes but produces secondary spermatocytes and spermatids with a 4 C DNA content, suggesting that the first and possibly also the second meiotic division is abolished. (ox.ac.uk)
Specific1
BACKGROUND AND AIMS: Trefoil factor family (TFF) peptides and the chromosome 11p15.5 mucin glycoproteins are expressed and secreted in a site specific fashion along the length of the gastrointestinal tract. (embl.de)