• Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. (frontiersin.org)
  • Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks. (mpg.de)
  • Of the major crop plants, hexaploid bread wheat ( Triticum aestivum L., genome composition AABBDD) and tetraploid pasta wheat ( T. durum Desf. (cropscience.org.au)
  • High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies. (springer.com)
  • A research team led by Dr. Zhang Heng and Dr. Zhu Jiankang from Shanghai Center for Plant Stress Biology of Chinese Academy of Sciences has sequenced the genome of broomcorn millet. (isaaa.org)
  • These bacterial genes are integrated into the genome of a plant cell at the moment of infection by the bacterium. (databasefootball.com)
  • While transformation procedures have become routine for a growing number of plant species, the random introduction of complex transgenenic DNA into the plant genome by current methods generates unpredictable effects on both transgene and homologous native gene expression. (bioone.org)
  • The use of site-specific recombination to remove undesired DNA from the plant genome and concomitantly, via excision-mediated DNA rearrangement, switch-activate by choice transgenes of agronomical, food or feed quality traits provides a versatile "transgene maintenance and control" strategy that can significantly contribute to the transfer of transgenic laboratory developments into farming practice. (bioone.org)
  • This review focuses on recent reports demonstrating the elimination of undesirable transgenes (essentially selectable marker and recombinase genes) from the plant genome and concomitant activation of a silent transgene (e.g., a reporter gene) mediated by different site-specific recombinases driven by constitutive or chemically, environmentally or developmentally regulated promoters. (bioone.org)
  • The GMO stands for " genetically modified organism ", meaning that something from an organism's genome (plant, animal or bacteria) is changed or modified. (geneticeducation.co.in)
  • Previous work in our lab had shown that turnip and Arabidopsis , both members of the Brassicaceae family of plants, were valuable species for generating HRs producing foreign proteins. (databasefootball.com)
  • Expression of open reading frame (ORF) VI of cauliflower mosaic virus (CaMV) in transgenic Arabidopsis thaliana caused a typical syndrome characterised by leaf chlorosis, vein clearing, plant stunting and reduced fertility. (psu.edu)
  • Previous work identified a barley UDP-glucosyltransferase gene (HvUGT13248) that detoxifies deoxynivalenol (DON) by the conversion to DON-3-O-glucoside (D3G) in transgenic yeast and Arabidopsis. (usda.gov)
  • One understudied opportunity is using feral crops-plants that have escaped and persisted without cultivation-as a source of genetic diversity, which could build resilience in domesticated conspecifics. (weizmann.ac.il)
  • In some cases, however, feral plants vigorously compete with crops as weeds, challenging food security. (weizmann.ac.il)
  • This paper examines the safety implication of recent revelations on the recombination hotspot of the cauliflower mosaic viral (CaMV) promoter, which is in practically all current transgenic crops released commercially or undergoing field trials. (i-sis.org.uk)
  • As a precautionary measure, we strongly recommend that all transgenic crops containing CaMV 35S or similar promoters which are recombinogenic should be immediately withdrawn from commercial production or open field trials. (i-sis.org.uk)
  • All products derived from such crops containing transgenic DNA should also be immediately withdrawn from sale and from use for human consumption or animal feed. (i-sis.org.uk)
  • That is all the more urgent as CaMV promoter is in practically all transgenic crops already released commercially or undergoing field trials. (i-sis.org.uk)
  • Trait stacking in transgenic crops: challenges and opportunities. (holliseden.com)
  • In recent times, there was a speedy improve within the planting of transgenic crops with stacked traits. (holliseden.com)
  • With the quest to grow more and better food to meet the demand of our fast growing world population, genetic engineering of crops has become a new platform in addition to plant breeding. (intechopen.com)
  • Research continues to develop and test new transgenic plants using genes provided by collaborators. (usda.gov)
  • Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)
  • The expression of genes encoded within the T-DNA promotes the development and production of roots at the site of infection on most dicotyledonous plants. (bioone.org)
  • For higher efficiency, plants are raised with genes from mammals, humans and microorganisms which don't occur naturally in plant. (unios.hr)
  • The expression of new genes allow plants to develop metabolism and resistances to pollutants in environment. (unios.hr)
  • Various approaches to avoid or eliminate undesirable transgenes, most notably selectable marker genes used in plant transformation, have recently been developed. (bioone.org)
  • These approaches include cotransformation with two independent T-DNAs or plasmid DNAs followed by their subsequent segregation, transposon-mediated DNA elimination, and most recently, attempts to replace bacterial T-DNA borders and selectable marker genes with functional equivalents of plant origin. (bioone.org)
  • Current trends and future prospects for optimization of excision-activation machinery and its practical implementation for the generation of transgenic plants and plant products free of undesired genes are discussed. (bioone.org)
  • On the other hand, leaves of plants grown in UV-B-enriched light had a more stable and long-lasting increase in the expression of these genes and also showed a stronger increase in leaf epidermal flavonol content. (bvsalud.org)
  • was explored by engineering wheat plants for constitutive expression of pathogenesis-related (PR) protein genes. (elsevierpure.com)
  • No expression of the chi11 and hpt genes, controlled by the CaMV 35S promoter, was detected in T 1 plants. (elsevierpure.com)
  • Traits such as herbicide, disease and insect resistance, improved tolerance to cold, salt and drought and accumulation of sugar and biomass have been some of the areas of interest as far as the application of transgenic sugarcane is concerned. (frontiersin.org)
  • Although there have been much interest in developing transgenic sugarcane there are only three officially approved varieties for commercialization, all of them expressing insect-resistance and recently released in Brazil. (frontiersin.org)
  • Crosses between Quest or ND20448 and two Conlon lines carrying either a tlp or a Tri12 transgene have continued, to determine whether the transgenes add to resistance from the breeding programs. (usda.gov)
  • While no effective HLB control methods or HLB resistance citrus cultivars have been commercialized, transgenic plant technologies have been recognized as a powerful tool to control the HLB disease. (springer.com)
  • An efficient genetic transformation protocol is a key step for using transgenic technologies to improve citrus quality, productivity, and resistance to abiotic and biotic stresses such as HLB. (springer.com)
  • were transformed with plasmids containing a hygromycin resistance gene, and plants were regenerated under a variety of selection regimes. (aber.ac.uk)
  • To determine the segregation of resistance among F2 phenotypes, the response of three- to four-leaf plants to imazethapyr was scored 3 wk after application as resistant (R, no imazethapyr symptoms), susceptible (S, death of plants), or intermediate (I, stunted plants). (weedscience.com)
  • The transgenic wheat show high levels of FHB type II resistance in the greenhouse point inoculation tests. (usda.gov)
  • We also performed spray inoculation tests on these transgenic wheat in the field across three consecutive years, and they also show high levels of FHB resistance. (usda.gov)
  • We used this root assay to show that transgenic barley overexpressing HvUGT13248 exhibit resistance to DON. (usda.gov)
  • We also introduced the HvUGT13248 transgene into the elite wheat cultivar Rollag, and the backcross-derived lines exhibited high levels of FHB resistance in the greenhouse and field tests, however, the FHB severity levels were not significantly different from Rollag. (usda.gov)
  • This result suggests that the FHB resistance mechanisms provided by the Fhb1 QTL and the HvUGT13248 transgene may overlap. (usda.gov)
  • Hydrogen peroxide (H2O2) is an important molecule that regulates antioxidant responses that are crucial for plant stress resistance. (bvsalud.org)
  • Plants not exposed to UV-B had a higher drought resistance in the form of higher relative water content of leaves. (bvsalud.org)
  • This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP. (elsevierpure.com)
  • The most common delivery method is biolistics based transformation but due to the high frequency of gene silencing linked with high copy transgenes and low edit rate in wheat, a large population of transgenic plants are needed for recovery of desired mutations. (isaaa.org)
  • Here we report successful development of transgenic wheat and barley overexpressing HvUGT13248 gene. (usda.gov)
  • Moreover, transgenic wheat carrying HvUGT13248 converted DON to D3G more rapidly than untransformed plants, and there was also reduced DON accumulation in the grains of the transgenic wheat harvested from the field tests. (usda.gov)
  • ENGLISH ABSTRACT: In most plants the efficiency of sucrose production and the systemic distribution thereof are the major determinants of growth, development and yield. (sun.ac.za)
  • Various hybrid or combination promoters have been constructed from the CaMV 35S promoter which led to improved expression of transgenes: double 35S promoters (10), a hybrid containing the core 19S promoter from CaMV and the 35S upstream enhancers (11), and combination of CaMV 35S with mannopine synthase elements (12), or with Adh1 - and ocs- promoter elements for expression in monocotyledons (13). (i-sis.org.uk)
  • Here, we present how altered root traits shown by transgenic plants impacted on physiological acclimation responses when submitted to severe water stress. (scirp.org)
  • Most of those merchandise have been fashioned by typical breeding, i.e. the crossing of transgenic plant (occasion) containing particular person transgenes with different occasion(s) containing single or double transgenic traits. (holliseden.com)
  • For a subset of five promoters, expression stability was further demonstrated in whole plant transgenics as well as in active nodules. (biomedcentral.com)
  • One major limitation associated with existing protocols when used on economically important citrus species is likely low plant regeneration frequencies. (springer.com)
  • The risk of transgene transfer into related plant species and consumers is another concern associated with the conventional transformation technologies. (bioone.org)
  • Besides hyperaccumulator herbaceous plants, several woody species are now considered of interest to this aim. (sisef.it)
  • Many woody plants are fast growing, have deep roots, produce abundant biomass, are easy to harvest, and several species revealed some capacity to tolerate and accumulate heavy metals. (sisef.it)
  • Genetically modified plant species or bacterial strains are created for various applications. (geneticeducation.co.in)
  • We performed oral toxicity tests in mice using various plant species as potential bioreactors and evaluated the immunogenicity of the resulting edible vaccine candidate. (bvsalud.org)
  • Exposure to herbal remedies pre- azo reduction in vivo to yield the car- sibly breast cancer), but at present pared from plant species of the ge- cinogen benzidine. (who.int)
  • Researcher Narayanan Narayanan from Donald Danforth Plant Science Center and colleagues developed biofortified cassava with higher levels of iron as reported in Nature Biotechnology . (isaaa.org)
  • It said something about anti-GMO, and being an educator specializing in plant biotechnology I clicked the link. (biofortified.org)
  • Christou P y Klee H. Handbook of plant biotechnology (volume 2). (unavarra.es)
  • A comprehensive treatise on new developments in biotechnology, the authors of Biotechnology and Safety Assessment, 3e, bring readers an up-to-date review of food safety issues, pre-clinical safety and development of new foods and drugs, plant biotechnology, food allergies and safety assessment, and consumer benefits with regard to genetically modified food. (elsevier.com)
  • PCR reactions were performed on the genomic DNA of putative transgenic maize plants using primers targeting the VHb gene. (biomedcentral.com)
  • Putative red rice hybrids were detected among volunteer plants in the following year. (weedscience.com)
  • Our data associate the interaction between Mn-SOD transgene overexpression and the UV-B treatment with a stress response. (bvsalud.org)
  • The four transgenes co-segregated in the T 1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. (elsevierpure.com)
  • The first gene being tested in transgenic American chestnut encodes an enzyme called oxalate oxidase . (esf.edu)
  • BADH enzyme activity was enhanced 8-fold in transgenic carrot cell cultures, grew 7-fold more, and accumulated 50- to 54-fold more betaine (93-101 μmol g−1 dry weight of β-Ala betaine and Gly betaine) than untransformed cells grown in liquid medium containing 100 mm NaCl. (upenn.edu)
  • Using a plasmid encoding a hygromycin phosphotransferase enzyme of higher specific activity (pROB5) resulted in the production of significantly more transgenic plants. (aber.ac.uk)
  • Thus, targeting the molecular mechanisms correlated with drought tolerance without reducing productivity is a challenge for plant breeding. (scirp.org)
  • Michael Wassenegger is associate professor in molecular biology at the University of Heidelberg, deputy director of the AlPlanta Institute for Plant Research and head of the AlPlanta Epigenetics Department. (degruyter.com)
  • R1- To k now, understand and apply the molecular underpinnings and biotechnological techniques in the production of recombinant proteins in plants. (unavarra.es)
  • Erickson L, Yu WJ, Brandle J y Rymerson R. Molecular farming of plants and animals for human and veterinary medicine. (unavarra.es)
  • Fischer R y Schillberg S. Molecular farming: plant-made pharmaceuticals and technical proteins. (unavarra.es)
  • Written by internationally recognized molecular biologists, plant agronomists, microbiologists, toxicologists, nutritionists, and regulatory authorities, this third edition is an excellent and authoritative resource, making it a valuable resource to any biomedical library or scientific bookshelf. (elsevier.com)
  • Unlike other plant tissues which are grown in vitro , they have an indefinite growth without any phytohormone. (databasefootball.com)
  • In vitro transgenic carrot cells transformed with the badh transgene were visually green in color when compared to untransformed carrot cells, and this offered a visual selection for transgenic lines. (upenn.edu)
  • The use of in vitro cultures, the role of bacteria and mychorrhizas, the powerful tool of genetic engineering, are some of the aspects focused in this paper that open prospects of global relevance for a better understanding of the processes related to the uptake of heavy metals by woody plants. (sisef.it)
  • Upon inoculation, mature Pospiviroidae RNA molecules enter the plant cell nucleus. (degruyter.com)
  • After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T 1 , T 2 and T 3 generations expressing the tlp gene than in non-transformed control plants. (elsevierpure.com)
  • Rhizobium rhizogenes is a bacterium that infects wounded plant stems. (databasefootball.com)
  • In this review, we provide an up-to-date overview of the current knowledge of how A. rhizogenes induces root formation, on the new uses for A. rhizogenes in tissue culture and composite plant production (wild-type shoots with transgenic roots), and the recent development of a disarmed version of A. rhizogenes for stable transgenic plant production. (bioone.org)
  • Unlike transgenic plants that are generally grown in soil and produce pollen or seeds which can convey the transgene into the environment, transgenic HRs are fully contained in vessels and would be unable to grow outside. (databasefootball.com)
  • 2014). With the help of several collaborators, we are evaluating potential impacts of transgenic chestnut on soil fungi, aquatic insects, terrestrial insects, other wildlife, persistence in the environment, tree growth rates, pollen flow, and surrounding plant communities. (esf.edu)
  • These roots differ from normal plant roots by not growing towards the soil and by carrying many root hairs, hence their name of "hairy roots" (HRs). (databasefootball.com)
  • These findings are described in the article entitled Enhanced production of recombinant human gastric lipase in turnip hairy roots , published in the journal Plant Cell, Tissue and Organ Culture (PCTOC) . (databasefootball.com)
  • Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. (biomedcentral.com)
  • Plant roots are dynamic structures involved in diverse developmental and physiological processes [ 1 ]. (biomedcentral.com)
  • This bacterium induces the neoplastic growth of plant cells that differentiate to form "hairy roots. (bioone.org)
  • Hairy roots are induced by the incorporation of a bacterial-derived segment of DNA transferred (T-DNA) into the chromosome of the plant cell. (bioone.org)
  • A key characteristic of hairy roots is their ability to grow quickly in the absence of exogenous plant growth regulators. (bioone.org)
  • As a result, hairy roots are widely used as a trans-genic tool for the production of metabolites and for the study of gene function in plants. (bioone.org)
  • Transgenic lines developed in Conlon are being crossed to resistant lines developed by the breeding programs. (usda.gov)
  • Lines included Conlon, Golden Promise, the resistant checks Quest and CI4196, four transgenic-null pairs derived from crosses between primary Golden Promise transgenics and Conlon, and one Golden Promise primary transgenic-null pair. (usda.gov)
  • Control measures are discussed including development of a highly resistant transgenic plum line. (apsnet.org)
  • To confirm the presence of the transgenes, DNA from npt II transgenic cucumber plants was analysed by polymerase chain reaction after transplanting resistant regenerated plants. (researchsquare.com)
  • Transgenic carrot plants expressing BADH grew in the presence of high concentrations of NaCl (up to 400 mm), the highest level of salt tolerance reported so far among genetically modified crop plants. (upenn.edu)
  • Demonstration of plastid transformation via somatic embryogenesis utilizing non-green tissues as recipients of foreign DNA for the first time overcomes two of the major obstacles in extending this technology to important crop plants. (upenn.edu)
  • Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. (nature.com)
  • Transgenic lines overproducing the yeast-derived invertase in either the vacuole (Vac-Inv) or apoplast (Apo-Inv) were utilised to analyse the effect of the altered sugar levels in sink and source organs on the expression of sugar transporters, as well as the endogenous cell wall invertase and inhibitors in these plants. (sun.ac.za)
  • Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
  • His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)
  • 1 - Plants as chemical and pharmaceutical factories: production of therapeutic proteins, antibodies, vaccines, enzymes and proteins of industrial use. (unavarra.es)
  • Faye L y Gomord V. Recombinant proteins from plants. (unavarra.es)
  • The potential commercialization of genetically modified herbicide-tolerant (GMHT) oilseed rape in Europe raises various concerns about their potential environmental and agronomic impacts, especially those associated with the escape of transgenes. (cambridge.org)
  • Genetically modified food simply means that the original DNA (deoxyribonucleic acid) structure of plants has been altered or tempered with. (intechopen.com)
  • In recent years significant progress has been made in identifying native plants and developing genetically modified tree plants for the remediation of heavy-metal polluted environment. (sisef.it)
  • In light of these issues, it is appropriate to develop rheological methods that reveal how plant tissue mechanical properties are affected in genetically modified switchgrass with reduced lignin content. (biomedcentral.com)
  • Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. (scielo.br)
  • However, genetic transformation of sugarcane is a very laborious process, which relies heavily on intensive and sophisticated tissue culture and plant generation procedures that must be optimized for each new genotype to be transformed. (frontiersin.org)
  • On the other hand, transgenic plants produced from mature tissue via genetic transformation can drastically shorten flowering and fruiting time and thus speed up field evaluation studies. (springer.com)
  • The genetic transformation of plants and the subsequent development of transgenic lines with disturbed sugar metabolism have made an unprecedented impact on the study of sugar translocation and -partitioning. (sun.ac.za)
  • The relentless progress of the disease in Europe and the severity of the disease led to the development of the Sharka International Working Group in the 1970's within the framework of the European Plant Protection Organization (EPPO), which allowed coordination of research and a free flow of information between countries. (apsnet.org)
  • The FHB severity of the transgenic lines were reduced by up to 91% compared to untransformed lines. (usda.gov)
  • The 2013 North Dakota transgenic field trials consisted of 15 barley lines, tested in three misted and three non-misted replicates. (usda.gov)
  • i) The virulence of the biocontrol agent must be enhanced to overcome evolutionary barriers either by mixing with synergistic chemicals or with one or more organisms, and/or by mutagenic or transgenic enhancing of virulence of the biocontrol fungus. (weizmann.ac.il)
  • This is partly because environmental risk assessment for transgenic plants is new and partly because the social context in which regulatory decisions about transgenic organisms must now be made is dramatically different from the social context in which these agencies are accustomed to working. (nationalacademies.org)
  • The Animal and Plant Health Inspection Service (APHIS) began its involvement in the regulation of transgenic organisms in the mid-1980s. (nationalacademies.org)
  • As the little bumps on HRs resembled calli that often develop on plant tissues treated with hormones, we proposed naming this new HR-based protein production system "rhizocalli. (databasefootball.com)
  • As opposed to the expense and complication of relative humidity control, solvent-submersion rheological methods effectively reveal fundamental structure/property relationships in plant tissues. (biomedcentral.com)
  • UV-B also retarded growth and suppressed acclimation responses in highly H2O2-overproducing transgenic plants. (bvsalud.org)
  • Furthermore, development of a notification process that focuses on plant ecology was an important step in effectively streamlining the field-testing process. (nationalacademies.org)
  • For instance, the transformation of plants with a yeast-derived invertase targeted to different subcellular compartments has led to the elucidation of several key aspects of sugar metabolism, including phloem loading mechanisms, the regulation of photosynthesis by sugars, the importance of sugar-metabolism compartmentation with regards to sucrose biosynthesis, storage and distribution, as well as the role of cell-wall invertase in phloem unloading and sink strength. (sun.ac.za)
  • Our new modules enable ligand-programmable multi-channel CID systems for plant and eukaryotic synthetic biology that can empower new plant-based and microbe-based sensing modalities. (nature.com)
  • Since then, she has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and her main field of interest is RNA-interference (RNAi) and viroid biology in plants. (degruyter.com)
  • In this way, we evaluated the effects of water deficit progress on AtDREB2A-CA transgenic cotton plant responses, driven by the stress-inducible rd29 promoter. (scirp.org)
  • Research groups from Heinrich Heine University Düsseldorf (HHU) and the University of Münster (WWU) have discovered a new switch that plants use to control their responses to iron deficiency. (isaaa.org)
  • Only root cap and epidermis are in direct contact with the rhizosphere, but plant responses to stimuli also involve internal cell types such as cortex, endodermis, pericycle and vasculature [ 3 ]. (biomedcentral.com)
  • HBsAg was the first viral antigen chosen to be produced in transgenic plants, firstly in tobacco [3] and subsequently in lupin callus and lettuce adapted to colder climates [4]. (scielo.br)
  • The yeast-invertase Suc2 gene, from Saccharomyces cere visiae , was overexpressed in either the cytosol, vacuole or apoplast of transgenic tobacco plants. (sun.ac.za)
  • Vertical gene flow from transgenic oilseed rape to non-GM counterparts may also have an impact on farming and supply chain management, depending on labelling thresholds for the adventitious presence of GM material in non-GM products. (cambridge.org)
  • The oxalate oxidase gene is also being tested by several other researchers to enhance pathogen tolerance in plant systems such as peanut , soybean , sunflower , and oilseed rape and also to possibly increase salt tolerance in tomato . (esf.edu)
  • HRs can be cut off an infected plant and grown in a liquid culture medium in flasks or in Petri dishes (figure 2). (databasefootball.com)
  • The effect was more prominent in plants grown under UV-B-enriched light than in those grown under UV-A. Parameters particularly affected were internode lengths, petiole lengths and stem stiffness. (bvsalud.org)
  • In fact, the bending angle of the 2nd internode was found to increase as much as 67% and 162% for plants grown in the UV-A- and UV-B-enriched treatments, respectively. (bvsalud.org)
  • As indicated in Chapter 1 , regulatory agencies charged with assessing the safety of transgenic plants face a daunting task. (nationalacademies.org)
  • APHIS's regulatory process has never led to the release of a transgenic plant that clearly caused environmental damage. (nationalacademies.org)
  • Pumpkin is one of the well-known edible plants and has substantial medicinal properties due to the presence of unique natural edible substances. (researchgate.net)
  • The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
  • Microscopy will be used to study the synthesis, trafficking and deposition of the tagged PIN-b protein in the developing and mature grain of the transgenic lines using the epitope tag as a basis for immunogold labelling. (cropscience.org.au)
  • All these phenotypes correlated with expression of ORF VI in three lines of transgenic plants which were produced independently, with different Ti-plasmid derived vectors and with different selective markers. (psu.edu)
  • These transgenic lines displayed varying increases in invertase activity, altered sugar levels and consequently disturbed sink-source interactions and sugar partitioning. (sun.ac.za)
  • systems to optimize the expression of the transgene. (unavarra.es)
  • In these aspects, the ORF VI transgenic plants resembled late flowering mutants. (psu.edu)
  • Plant biologists subsequently discovered that many phytohormones regulate signaling through CID mechanisms. (nature.com)
  • 0 - Transgenic plants: concept and methods of plant transformation. (unavarra.es)
  • The purpose of the present article is to discuss various medicinal and biological potentials of pumpkin that can impart further research developments with this plant for human health benefits. (researchgate.net)
  • Homoplasmic transgenic plants exhibiting high levels of salt tolerance were regenerated from bombarded cell cultures via somatic embryogenesis. (upenn.edu)
  • Electrophoresis, western blot analysis and GUS staining have also been used to compare the patterns of accumulation of PINs in the developing grain of the transgenic and control wheats. (cropscience.org.au)
  • Plant regeneration was highest under discontinuous selection, but resulted in the production of significant numbers of escapes. (aber.ac.uk)
  • Plants as factories for protein production. (unavarra.es)
  • To see how reduced oxalate affects the fungus' ability to attack a plant, view the following time-lapse video of an "Apple test" used to measure fungal virulence (long version viewable on YouTube ). (esf.edu)
  • Transcription occurs from a nonintegrated, circular minichromosome in the nucleus of the plant cell, and virion DNA is synthesised in the cytoplasm by reverse transcription of the 35S RNA transcript (4, 5). (i-sis.org.uk)
  • When PSTVd enters the plant cell, a specific signal seems to direct it into the nucleus. (degruyter.com)
  • Each infected cell then develops into a HR. When a foreign gene is added to the bacterium, it can also be transferred to the infected plant cell and expressed in the developing HR, which can then be propagated on large scale. (databasefootball.com)
  • Experimental basics are provided for the novice, where the intention is to promote collaboration between plant biologists and materials scientists. (biomedcentral.com)