Transformed cell lines. Medical search. Frequent questions

The transformed phenotype of these Schwann cell lines was determined by both integration of SV40 viral DNA sequences into the cellular genome and active synthesis of the large T antigen of SV40. (cornell.edu)
In addition, similar transformations with SV40 virus containing a temperature-sensitive mutation in the large T gene yielded Schwann cell lines with transformed phenotype which were temperature-sensitive. (cornell.edu)
15. c-Myc Sustains Transformed Phenotype and Promotes Radioresistance of Embryonal Rhabdomyosarcoma Cell Lines. (nih.gov)
This has led to the hypothesis proposed by Loeb (6) that cancer cells exhibit a mutator phenotype and that the loss of genome stability function(s) early during tumor development could produce an increase in mutation rate in the affected cells. (aacrjournals.org)
MPI cells, a non-transformed line that closely mimics AM phenotype, were treated with mass-, surface area- (s.a.), and particle number- (p.n.) equivalent doses of respirable asbestiform and non-asbestiform riebeckite/tremolite EMP (median lengths 4.5-5.5 microm) for 24 h with or without LPS (5 ng/ml). (cdc.gov)

Primary cultures derived from uninfected mule deer brain tissue were transformed by transfection with a plasmid containing the simian virus 40 genome. (nih.gov)
No cross-reaction with DNA of herpes simplex virus, human cytomegalovirus, human T cell leukemia/lymphoma virus I and II, or polyoma virus was detected in the genome of immortalized cell lines by Southern blot hybridization. (rupress.org)
Furthermore B and T cell lines were established that appear to be free of Epstein-Barr virus genome. (rupress.org)
Normal cells have multiple mechanisms to recognize and repair DNA damage before mutations arise (5) , serving to maintain the integrity of the genome. (aacrjournals.org)

C8-B4 is a spontaneously transformed microglial clone of a cell line originally derived from the cerebellum of an 8-day-old mouse ( Mus musculus ) in 1984. (olympus-lifescience.com)
These cells lines were obtained from spontaneously transformed primary cell cultures without genetic, viral or chemical manipulation so they can serve as mouse models for studying the natural process of oncogenesis. (nih.gov)
Spontaneously transformed primary cell cultures were generated from isogenic mouse strain that has a low propensity for epithelial tumors in vivo therefore, not involving other mouse strains potentially influencing the genetic background. (nih.gov)

Biosynthesis of myelin-associated proteins in simian virus 40 (SV40)-transformed rat Schwann cell lines. (cornell.edu)
In order to study the biosynthesis of myelin-associated proteins in Schwann cells, we have induced proliferation of cultured Schwann cells from neonatal rat sciatic nerve by transformation with Simian Virus 40 (SV40). (cornell.edu)
Characterization of a simian virus 40-transformed Fanconi anemia fibroblast cell line. (xenbase.org)

These cell lines were generated without viral, chemical or genetic manipulation and thus can serve as mouse models for studying the natural process of oncogenesis and as mouse models of human cancers. (nih.gov)
However, a limitation of any lytic viral expression system, including BEVS, is that death and lysis of infected insect cells terminates protein production. (nih.gov)
This results in interruption of protein production and higher production costs due to the need to set up new infections, maintain uninfected cells, and produce pure viral stocks. (nih.gov)
We provide our customers with world-class development and manufacture of mammalian and microbial-based therapeutic proteins, plasmid DNA (pDNA), viral vectors, and genetically engineered cells (cell therapy). (thechiefsdigest.com)
The CDC guidelines allows BSL-2 use for growth of all cell lines free of infectious agents, including growth of replication incompetent viral vectors for gene delivery. (edu.lb)

To facilitate in vitro studies of CWD, we have developed a transformed deer cell line that is persistently infected with CWD. (nih.gov)
Studies have revealed a wide range of phenotypic differences among OS cell lines in terms of their in vivo tumorigenicity and in vitro colony-forming abilities. (nature.com)
To this end, we tested the tumorigenicity and anoikis resistance of OS cell lines both in vitro and in vivo. (nature.com)
3 Previous studies that tested different OS cell lines identified distinct phenotypes in terms of in vivo tumorigenicity and in vitro colony-forming capacity. (nature.com)
Monocellular layers of tumors cultivated in vitro and mouse xenografts derived from those cells have been the standard toolkit for cancer biologists for decades. (aacrjournals.org)
Moreover, regorafenib resistant cells also exhibit significantly augmented in vitro and in vivo migration capacity which could be reversed by TGF-β type 1 receptor (TGFb -R1) inhibition. (frontiersin.org)
The CD23 antibody, clone EBVCS-5, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with an in vitro-transformed EBV cell line. (bdbiosciences.com)
TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorrhagic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. (biolegend.com)
17. Deguelin inhibits the migration and invasion of U-2 OS human osteosarcoma cells via the inhibition of matrix metalloproteinase-2/-9 in vitro. (nih.gov)
Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro med. (emf-portal.org)
Línea celular eucariótica obtenida en una fase estacionaria o de quiescencia que experimenta, en cultivo, una transformación hacia un estado de crecimiento descontrolado semejante a un tumor in vitro. (bvsalud.org)
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. (bvsalud.org)

Today, scientists can use stem cells to cultivate epithelial cell organoids, i.e. cell clusters that replicate natural tissues. (mpg.de)

The cell lines were characterized cytogenetically during their transformation from normal to spontaneously immortalization and were found to recapitulate many of the changes observed in human cancer cells such as the deregulation of oncogenes (Myc, Mdm2) and tumor suppressor genes (Cdnk4a/Ink4a/p16, Rb). (nih.gov)
Regorafenib resistant cells (RRCs) also show increased expression of several mesenchymal genes along with an induction of CD24 and CD133 cancer stem cell markers. (frontiersin.org)
None of the genes studied were amplified in these transformed cells. (cdc.gov)
Testing additional individual\produced pancreatic tumor cells reveals particular genes (and genes that are essential for proliferation such as for example and by dealing with with APE1 NIBR189 redox\particular inhibitor, APX3330. (mingsheng88.org)
Panc10.05 cells, produced from an initial PDAC tumor, exhibited similar leads to Pa03C cells, with eight from the 12 genes displaying similar changes in expression (Fig.?6A). (mingsheng88.org)
Generating a substantial quantity of data, we primarily corrected for batch results using cell routine\annotated genes. (mingsheng88.org)
Polymorphisms in transforming growth factor-beta-related genes ALK1 and ENG are associated with sporadic brain arteriovenous malformations. (medlineplus.gov)

However, RNA interference studies have shown that depletion of IRF4, rather than of IRF7, dramatically decreases the endogenous level of BIC by up to 70% in EBV- or human T-cell leukemia virus type 1 (HTLV1)-transformed cell lines and results in apoptosis and reduction of proliferation rates that are restored by transient expression of miR-155. (nih.gov)
However, these models fail to reproduce the tumor microenvironment and tumor cell interactions with the innate immune system, both of which are integral to tumor development, proliferation, and metastasis ( 1 ). (aacrjournals.org)
Fusion of mouse L929 cytoplasts with human peripheral blood lymphocytes induced lymphocyte proliferation that gave rise to lymphoid cell lines of B and T cell origin with unlimited growth potential. (rupress.org)
Each establishment of lymphoid cell lines was preceded by a proliferation phase 2 wk after cytoplast/cell fusion, which appears to be a necessary step in the immortalization process. (rupress.org)

In the past, we were dependent on HeLa cells - a human cell line cultivated from cervical cancer - for infection experiments with Chlamydia. (mpg.de)
For the past seven decades, the cells of Henrietta Lacks, a Black American woman who died of cervical cancer, have saved countless lives, and made numerous scientific breakthroughs possible, such as the human papillomavirus and polio vaccines, drugs for HIV treatment, together with cancer and COVID-19 research. (un.org)

However, research on cellular transformation has largely relied on fibroblast cells which are not of epithelial origin and therefore, may not reflect the changes that lead to epithelial oncogenesis. (nih.gov)
Cell transformation and gene expression induced by metal working fluid in BALB/c-3T3 cells. (cdc.gov)
These results indicate that MWF is capable of inducing morphological transformation in BALB/c-3T3 cells and that alteration in c-jun and c-fos expression in associated with MWF-induced transformation. (cdc.gov)

Moreover, the endogenous levels of the miR-155 target, SHIP1, are consistently elevated in EBV- and HTLV1-transformed cell lines stably expressing shIRF4. (nih.gov)
By using the CsIV vankyrin gene family, protein production in the vankyrin-enhanced BEVS (VE-BEVS) was increased by a factor of 4- to 15-fold by either coexpressing the vankyrin protein from a dual BEVS or by providing its activity in trans by expressing the vankyrin protein from a stably transformed cell line. (nih.gov)

The MDB(CWD2) cell line was positive for fibronectin and negative for microtubule-associated protein 2 (a neuronal marker) and glial fibrillary acidic protein (an activated astrocyte marker), consistent with derivation from brain fibroblasts (e.g., meningeal fibroblasts). (nih.gov)
Despite the expression of MAG and CNPase, these Schwann cells did not synthesize PO the major protein of peripheral myelin. (cornell.edu)
Genetic methods to slow or prevent cell death while maintaining high-level, virus-driven protein production could dramatically increase protein yields. (nih.gov)
A gene family from the insect virus Campoletis sonorensis ichnovirus (CsIV) was discovered that delays lysis of baculovirus-infected cells, thereby significantly enhancing recombinant protein production in the BEVS system. (nih.gov)
Activation of pathways in recipient cells was determined at gene and protein expression levels. (cancerindex.org)
From the pilot screen of the approved drug library, multiple compounds demonstrated similar strong cytotoxic effects across all four of the cells derived from patients with pancreatic cancer to include proteasome inhibitors bortezomib and carfilzomib, histone deacetylase inhibitor romidepsin, and protein synthesis inhibitor homoharringtonine. (scienceboard.net)
Organelles called ribosomes serve as the protein-assembly motors within cells. (scripps.edu)
Bacteria have related, but somewhat more direct systems for addressing halted ribosomes and their protein fragments, according to the Cell report. (scripps.edu)
The next step will be to find out whether the defective virulence of strep varieties lacking Rqc2 is primarily a consequence of their failure to recycle protein fragments inside the cell. (scripps.edu)
Fas (Fibroblast-associated), also known as Apo-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. (rndsystems.com)
It has been previously demonstrated that the atypical BC human cell line NCI-H720 is sensitive to everolimus (E), an m-TOR inhibitor, in terms of cell viability reduction, with a G0 cell-cycle arrest and a Cyclin D1 protein reduction. (endocrine-abstracts.org)
This protein is found on the surface of cells, especially in the lining of developing arteries. (medlineplus.gov)
In the case of the ACVRL1 protein, the ligand is called transforming growth factor beta. (medlineplus.gov)

We previously showed that some V-ATPase subunits are enriched in glioma stem cells and in patients with poor survival. (cancerindex.org)
In this study, we show that regorafenib treatment activates Wnt/β-catenin signaling only in hepatoblast-like HCC cell lines and induces enrichment of markers associated with hepatic stem/progenitor cells. (frontiersin.org)
The recovery of physiological functionality, which is commonly seen in tissue mimetic three-dimensional (3D) cellular aggregates (organoids, spheroids, acini, etc.), has been observed in cells of many origins (primary tissues, embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and immortal cell lines). (mdpi.com)
Epithelial cells lining the digestive tract represent a highly organized system built up by multipotent stem cells. (bioone.org)
The main problem in developing primary culture is the lack of structural markers specific to the stem cell compartment. (bioone.org)
The design of a microscopic multidimensional analytic system to record the expression profiles of biomarkers all along the living intestinal crypt should improve basic knowledge of the survival and growth of adult crypt stem cells, and the selection of totipotent embryonic stem cells capable of differentiating into intestinal tissues should facilitate studies of the genomic basis of endodermal tissue differentiation. (bioone.org)
Gene sets identified with oncogene cooperativity analysis regulate in vivo growth and survival of leukemia stem cells. (rochester.edu)
Cell stem cell. (rochester.edu)
The terms cytokine storm and CRS have both been used to describe similar syndromes of systemic inflammation that are associated with a variety of diseases and treatments, such as graft versus host disease (GVHD) after allogeneic stem cell transplant, macrophage activation syndrome (MAS)/hemophagocytic lymphohistiocytosis (HLH), idiopathic multicentric Castleman disease (iMCD), COVID-19, and as an adverse event associated with several monoclonal antibody infusions. (medscape.com)

Although no PrP(CWD) was detected in any of these cultures after two passes, dilution cloning of cells yielded one PrP(CWD)-positive clone out of 51. (nih.gov)
However, a few drugs displayed preferential cytotoxicity against 3D models over 2D cultures, which proved to be both cell and drug dependent. (scienceboard.net)
Measurements of the exposure system by the provider showed a non-uniformity of SAR for monolayer cell cultures of less than 30% and a temperature load of less than 0.03 °C per W/kg of SAR . (emf-portal.org)
You will find everything needed to grow and experiment on cell lines, whether immortalized, transformed or primary cell cultures. (edu.lb)

Gordon J, Rowe M, Walker L, Guy G. Ligation of the CD23,p45 (Blast-2, EBVCS) antigen triggers the cell-cycle progression of activated B lymphocytes. (bdbiosciences.com)
A B-cell- specific differentiation antigen, CD23, is a receptor for IgE (FcεR) on lymphocytes. (bdbiosciences.com)
Immortalization of human lymphocytes by fusion with cytoplasts of transformed mouse L cells. (rupress.org)
Fas is highly expressed in epithelial cells, hepatocyes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. (rndsystems.com)

Importantly, when we knocked down TGFβ-R1 using a lentiviral plasmid, regorafenib resistant cells entered senescence indicating that this pathway is important for their survival. (frontiersin.org)
Using an assay for repair based on host cell reactivation of UV-damaged plasmid DNA, cells exposed to hypoxia and low pH were found to have a diminished capacity for DNA repair compared with control cells grown under standard culture conditions. (aacrjournals.org)

Furthermore, in this test scenario, several of the panel drugs were definitively cell line specific, notably cancer associated fibroblasts (CAF)-selective statins and cardiac glycosides, as well as pancreatic cancer cell-selective trametinib and disulfiram. (scienceboard.net)

Molecular biology of deregulated gene expression in transformed C3H/10T1/2 mouse embryo cell lines induced by specific insoluble carcinogenic nickel compounds. (nih.gov)
The substances were investigated by using an h-ras oncogene transformed rat embryo fibroblast cell line (5RP7). (anadolu.edu.tr)

Additionally, we quantified the expression of sensor proteins, including four kinases and seven cytoskeletal proteins, in OS cell lines. (nature.com)
We identified toggling between normal and transformed growth based on the expression pattern of rigidity-sensing proteins in OS cells. (nature.com)
A new study from the lab of Claudio Joazeiro, PhD , published online in the journal Cell on May 30, uncovers how simpler organisms-bacteria and archaea-manage the recycling of incomplete proteins. (scripps.edu)
We know that as cells are making proteins, this process is occasionally halted due to errors," says Joazeiro, who has joint appointments in the Scripps Research Department of Molecular Medicine in Jupiter, Florida, and the Center for Molecular Biology of Heidelberg University, in Germany. (scripps.edu)
So in our lab, we're asking how do cells sense this, and how do they disassemble these proteins and recycle the building blocks? (scripps.edu)
Previous studies had suggested that in some pathogenic bacteria, Rqc2 proteins had a different job, one that functioned outside the cell, helping attach the microbes to hosts. (scripps.edu)
Shortly after cells invented how to make proteins, they were also faced with determining how to deal with incompletely made proteins," Joazeiro says. (scripps.edu)

Patterns of gene expression are reduced in complexity in cell culture models, suggesting that heterogeneity is lost once a tumor is removed from a patient and cultured in the laboratory ( 1 ). (aacrjournals.org)
Gene expression profiles of renin producing cells in newborn and adult kidney isolated from Renin-YFP transgenic mice using FACS. (nih.gov)

9 Transformed growth or anoikis resistance refers to a state in which malignant tumor cells can evade context-dependent conditions and survive. (nature.com)

Carcinomas that arise from the epithelial cells lining organs lead to the most common cancers in humans. (nih.gov)

We further uncovered a novel TP53 mutation (R156P) in transformed OS cells, which acquired gain of function to inhibit rigidity sensing, thus sustaining transformed growth. (nature.com)

Epithelial cells from normal pig bladders proliferated when cocultured with lethally irradiated feeder cells of the LA7 rat mammary tumor line. (bioone.org)

3D ex vivo tumor models enable better recapitulation of in vivo biology features, such as cell-cell interactions, cell-matrix interactions, hypoxia, heterogeneity of tumor, drug penetration, and drug resistance, for putative in vivo characterization. (scienceboard.net)
13. 3-Hydroxyflavone inhibits human osteosarcoma U2OS and 143B cells metastasis by affecting EMT and repressing u-PA/MMP-2 via FAK-Src to MEK/ERK and RhoA/MLC2 pathways and reduces 143B tumor growth in vivo. (nih.gov)
This alteration in repair capacity may constitute an important mechanism underlying the genetic instability of cancer cells in vivo . (aacrjournals.org)

We utilized a sphere culture model, a soft agar assay, and soft and rigid hydrogel surface culture models to investigate the function of rigidity sensing in the tumorigenicity of OS cells. (nature.com)
Through the application of the NIH/3T3 tumorigenicity assay to DNA from a gastric carcinoma, we have identified a novel transforming gene, designated myeov (myeloma overexpressed gene in a subset of t[11;14]-positive multiple myelomas). (elsevierpure.com)
The NRU cytotoxicity assay procedure is based on the ability of viable cells to incorporate and bind neutral red (NR), a supravital dye. (nih.gov)
The methodology includes an initial 3D cell viability assay that is screened in a 384-well format prior to further miniaturization into a 1536-well. (scienceboard.net)

Moreover, activation of Wnt/β-catenin signaling via Wnt3a/R-Spo1 treatment protects these cells from regorafenib induced apoptosis. (frontiersin.org)
Apoptosis induction was more pronounced in asbestos -treated cells, compared to CF in all comparisons (mass/s.a./p.n. (cdc.gov)

We demonstrate that APE1 impacts modifying gene manifestation up to threshold of APE1 manifestation, demonstrating that it's not essential to knockout APE1 in cells to accurately research APE1 function completely. (mingsheng88.org)

Mr. Lacks said the family was moved to receive this historic recognition, honouring "a remarkable woman and the lasting impact of her HeLa cells. (un.org)

whole cell lysate. (abcam.com)
IP:THP-1 (treated with 80nM TPA / 100 ng/ml lipopolysaccharide / 300ng/ml Brefeldin A) whole cell lysate. (abcam.com)

Isolated crypts or epithelial cells retaining high viability can be prepared within a few h after tissue sampling. (bioone.org)
We previously demonstrated that the typical BC human cell line NCI-H727 is sensitive to Everolimus, in terms of cell viability reduction, while the atypical human BC cell line NCI-H720 is not. (endocrine-abstracts.org)
We assessed viability and apoptotic response, lactate dehydrogenase (LDH) and cytokines in cell supernatants. (cdc.gov)

Moreover, the combination of programmed cell death ligand 1 (PD-L1) inhibitor atezolizumab and VEGFR inhibitor bevacizumab was also recently approved for first-line treatment for advanced HCC. (frontiersin.org)

J Cell Biol (1986) 103 (3): 795-805. (rupress.org)

To study the transforming potential of MWF, BALB/c-3T3 cells were exposed to MWF at varying concentrations for 24 hours. (cdc.gov)

Furthermore, the level of IRF4 mRNA is significantly correlated with that of BIC in adult T-cell lymphoma/leukemia (ATLL) tumors. (nih.gov)
Since a substantial amount of normal size PO mRNA was present in these transformed Schwann cells, the lack of PO synthesis was apparently not the result of a deficiency of transcription. (cornell.edu)

In this report, we demonstrate that these SV40-transformed Schwann cells actively synthesize myelin-specific sulfatide, myelin-associated glycoprotein (MAG) and the glial cell marker 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase). (cornell.edu)
Here, we demonstrate that local and acute estrogen signaling in NCM transforms the auditory response properties of HVC neurons and that these actions appear to be mediated by a membrane-bound estrogen receptor. (jneurosci.org)
Although cyclin D1 overexpression represents a characteristic feature of all MM cell lines with t(11;14), our results demonstrate aberrant expression of a second putative oncogene in a subset of these cases, due to juxtaposition to IgH enhancers. (elsevierpure.com)
Experiments with the supF20 mutagenesis system demonstrate that extracts from Bloom's syndrome (BS) cells are unable to use microhomology elements within the supF20 gene to restore supF function after the induction of a double-strand break (DSB). (nih.gov)
On the other hand, regorafenib resistant cells established by long-term regorafenib treatment demonstrate diminished Wnt/β-catenin signaling activity while TGF-β signaling activity of these cells is significantly enhanced. (frontiersin.org)

The team suspects that the difference is mainly due to the fact that organoids resemble the natural structure of the epithelium: Their cells are not flat like cancer cells, but tightly packed together and three-dimensional, with a distinct inner and outer surface. (mpg.de)
Only a subset of cells is infected and subsequently pushed out of the epithelium by the surrounding cells, thus preventing tissue damage. (mpg.de)
Bronchial carcinoids (BC) are rare tumors originating from endocrine cells dispersed in the respiratory epithelium. (endocrine-abstracts.org)
GH is produced by normal and neoplastic endometrial cells and elevated levels have been observed in endometrial epithelium of patients with endometriosis and endometrial adenocarcinoma (EA). (endocrine-abstracts.org)
Background: Bronchial carcinoids (BC) are rare tumors originating from endocrine cells dispersed in the respiratory epithelium. (endocrine-abstracts.org)
Introduction: Neuroendocrine tumours (NETs) are heterogeneous neoplasms arising from neuroendocrine cells spread in the respiratory and gastro-entero-pancreatic epithelium. (endocrine-abstracts.org)

[ 22 ] resulting in a positive feedback loop of T-cell activation and inflammatory cytokine release, a murine model study has suggested that the cytokines and factors that mediate the severity of CRS, IL-6, IL-1, and nitric oxide are not produced by the CAR-T cells but by recipient macrophages, and can be reversed by IL-1 blockade. (medscape.com)
Alveolar macrophages (AM) are the first-responders to inhaled particulates, participating in clearance and activating the other resident and recruited immunocompetent cells, which has an impact on the long-term outcomes. (cdc.gov)

The NIH Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative Cell Census Network (BICCN) has unveiled an atlas of cell types and an anatomical neuronal wiring diagram for the mammalian primary motor cortex, derived from detailed studies of mice, monkeys, and humans. (nih.gov)
We hypothesize that primary cells, which lack CBs, contain different phosphoisoforms of coilin compared with that found in transformed cells, which have CBs. (biologists.com)
Large oncosome (LO) vesicles were isolated from primary glioblastoma (GBM) neurospheres, or from patient sera, and co-cultured with primary neoplastic or non-neoplastic brain cells. (cancerindex.org)
A new 3D cell culture method has been developed and validated using m3D bioprinting technology for HTS therapeutic screening with primary cancer cells to advance the discovery of appropriate clinical chemotherapeutics. (scienceboard.net)
However, long-term primary culture of intestinal cells (up to 10 d) is still difficult despite progress in isolation methodologies and manipulation of the cell microenvironment. (bioone.org)

As a result, the high frequency of mutations found in cancer cells cannot be accounted for by the low spontaneous mutation rate observed among somatic cells. (aacrjournals.org)

Yet, data suggest that the behavior of these lines has diverged substantially from the actual tumors from which they were derived. (aacrjournals.org)
In conclusion, our data suggest that HCC tumors with aberrant activation in the Wnt/β-catenin pathway, might have higher intrinsic regorafenib resistance and the inhibition of this pathway along with regorafenib administration might increase regorafenib-induced cell death in combinational therapies. (frontiersin.org)

Investigators at the NIH have created a collection of 45 mouse epithelial cancer cell lines derived from six organs: bladder, cervix, colon, lung, kidney, and mammary glands. (nih.gov)

Sherr E, Macy E, Kimata H, Gilly M, Saxon A. Binding the low affinity Fc ε R on B cells suppresses ongoing human IgE synthesis. (bdbiosciences.com)

When combined with regorafenib treatment, TGFβ-R1 inhibition also significantly decreased colony formation ability and augmented cell death in resistant spheroids. (frontiersin.org)
ITGA1RAB3Dwere significantly transformed in every four cell lines (Fig.?6D). (mingsheng88.org)

Healthy proliferating mammalian cells, when properly maintained in culture, continuously divide and multiply over time. (nih.gov)
Here, we monitor sensing, signaling from, and repairing of a single site-specific ICL in cell-free extract derived from Xenopus eggs and in mammalian cells. (xenbase.org)

Scientists from the Max Planck Institute for Infection Biology in Berlin have now investigated fallopian tube cells chronically infected with Chlamydia for many months. (mpg.de)

Thus, cell death and/or inhibition of cell growth decreases the amount of neutral red retained by the culture. (nih.gov)
Cytotoxicity is expressed as a concentration dependent reduction of the uptake of NR after chemical exposure, thus providing a sensitive, integrated signal of both cell integrity and growth inhibition. (nih.gov)
Inhibition of COP9-signalosome (CSN) deneddylating activity and tumor growth of diffuse large B-cell lymphomas by doxycycline. (rochester.edu)
5. Dihydromyricetin suppresses cell metastasis in human osteosarcoma through SP-1- and NF-κB-modulated urokinase plasminogen activator inhibition. (nih.gov)
6. Curcumin Analogue L48H37 Suppresses Human Osteosarcoma U2OS and MG-63 Cells' Migration and Invasion in Culture by Inhibition of upa via the JAK/STAT Signaling Pathway. (nih.gov)

Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching. (bdbiosciences.com)

In 3 of 7 multiple myeloma (MM) cell lines with a t(11;14)(q13;q32) and cyclin-D1 overexpression, Northern blot analysis revealed overexpression of myeov as well. (elsevierpure.com)

The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. (biolegend.com)

The National Cancer Institute Cancer Genetics Branch is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize mouse epithelial cancer cell lines. (nih.gov)
The availability of these mouse epithelial cancer cell lines should allow for a more accurate analysis of this process. (nih.gov)
Mouse xenografts of human tumor cell lines have had variable, and often poor, predictive power in the translation of cancer therapeutics into clinical settings ( 2 ). (aacrjournals.org)
This breed of mouse lacks natural killer cells and is considered more immunodeficient than a nude mouse. (aacrjournals.org)
PDX models are maintained by passaging cells directly from mouse to mouse once the tumor burden becomes too high. (aacrjournals.org)

However, the mechanisms underlying this phenomenon have not been fully clarified.Aim: The aim of our study is to investigate the mechanisms of resistance to mTOR inhibitors in BC cells. (endocrine-abstracts.org)

However, the mechanism underlying the heterogeneity among OS cell lines is still unclear. (nature.com)

Our findings suggest a fundamental role of rigidity-sensing components in OS tumorigenicity as mechanotransduction elements through which cells can sense their physical microenvironment. (nature.com)

A variety of cell lines can be used in this model, including human colorectal adenocarcinoma cell line HT-29 (ATCC no. (scienceboard.net)
HTB-38) and human pancreatic epithelial carcinoma cell line PANC-1 (ATCC no. (scienceboard.net)

In human and other eukaryotic cells, when a ribosome jams, rescue factors split it open. (scripps.edu)

Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines. (hsanmartino.it)
Studies using only transformed human cell lines will not meet this requirement. (nih.gov)

Human spinal cord cell atlas provides foundation to study neurodegeneration, chronic pain, and other diseases. (nih.gov)
Background Chronic alcoholic beverages publicity can transform splice variant expression. (ecologicalsgardens.com)
This is the first time we have succeeded in reproducing the course of a chronic bacterial infection in mucosal cells. (mpg.de)

When progeny transform within the lungs and their energy deposits in tissue (and not fluid), the genetic material of cells lining the airways can be damaged. (cdc.gov)

Pearson's research led to a collaboration with a fellow postdoc, Georges Kohler, and supervisor César Milstein to develop monoclonal antibodies specific for cell surface antigens. (wikipedia.org)

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