Putative transformants were obtained using polyethylene glycol-mediated transformation of protoplasts. (usda.gov)
Tissue culture4
However, genetic transformation of sugarcane is a very laborious process, which relies heavily on intensive and sophisticated tissue culture and plant generation procedures that must be optimized for each new genotype to be transformed. (frontiersin.org)
Replicate plant cells or protoplasts that are placed under identical conditions of tissue culture do not always grow and differentiate to produce identical progeny (clones). (jrank.org)
The first step is to develop and optimize a tissue culture and transformation system (protocols to introduce foreign DNA to new plants) for UF strawberry lines, so that new plants can be regenerated from cells containing introduced DNA. (ufl.edu)
The genetic engineering helps in manipulation of foreign gene (DNA) and tissue culture is essential for genetic transformation. (biologydiscussion.com)
Regeneration1
Med målet att utvärdera en mindre restriktiv teknik för genmodifiering i sötpotatis (Ipomoea batatas), både gällande ekonomiska begränsningar såväl som det tid och energi bemödande som ett sådant projekt oundvikligen påbrår, har vi arbetat flitigt med att etablera optimerade parameters för protoplast isolation och fortsättningsvis också utforskat lovande protokoll för transformation och regeneration av dessa protoplaster, för att slutligen framställa nya genotyper. (essays.se)
Agrobacterium3
Since the early 1990's, different genetic transformation systems have been successfully developed in sugarcane, including electroporation, Agrobacterium tumefaciens and biobalistics. (frontiersin.org)
Agrobacterium cells with a genetically-modified plasmid, containing a gene for the desired trait and a marker gene, usually conferring antibiotic resistance, are incubated with protoplasts or small pieces of plant tissue. (jrank.org)
These protocols may be adapted to other plant species that are recalcitrant to Agrobacterium -mediated transformation. (biomedcentral.com)
Transgenic3
The effect of selection pressure on transformation frequency and copy number in transgenic plants of tall fescue (Festuca arundinacea Schreb. (aber.ac.uk)
Gweld gwybodaeth am bynciau ymchwil 'The effect of selection pressure on transformation frequency and copy number in transgenic plants of tall fescue (Festuca arundinacea Schreb. (aber.ac.uk)
In most of the field crops, transgenic plants are recovered at a very low frequency Hence, huge single cells or protoplasts have to be screened in the culture medium for identification and isolation of transgenic cells. (biologydiscussion.com)
Biolistic1
For Chlorella biolistic transformation is used. (dapzoi.com)
Methods1
This study explores methods for transformation of E. oleoabundans by electroporation. (wpi.edu)
Genes2
Take 100μg each of plasmid (35S : GFP) extracted from two Resin kits, and separate protoplasts from Arabidopsis thaliana by enzymatic hydrolysis, and transform foreign genes into protoplasts by PEG-mediated transformation for transient expression. (biocomma.blog)
Importantly, a robust multiplex prime editing platform is established for simultaneous editing of four to ten genes in protoplasts and up to eight genes in regenerated wheat plants at frequencies up to 74.5%, thus expanding the applicability of prime editors for stacking of multiple agronomic traits. (biomedcentral.com)
The size of the particles and the entry velocity must be optimized for each tissue, but avoiding the need to isolate protoplasts increases the potential for regenerating transformed plants. (jrank.org)
This transformation method will allow for the immediate observation of phenotypes in the haploid gametophytes of transformed plants, as well as the generation of stably transformed C. richardii lines for further analysis. (biomedcentral.com)
Genetic transformation2
Genetic transformation has the potential to improve economically important properties in sugarcane as well as diversify sugarcane beyond traditional applications, such as sucrose production. (frontiersin.org)
The genetic transformation can be achieved either through cell culture or protoplast culture. (biologydiscussion.com)
Stable1
Stable transformation was achieved at a rate of nearly 3% by regenerating entire gametophytes from single transformed cells from T 0 gametophytes on selective media. (biomedcentral.com)
Sugarcane2
Therefore, it remains a great technical challenge to develop an efficient transformation protocol for any sugarcane variety that has not been previously transformed. (frontiersin.org)
The objective of this review is to summarize current techniques and state of the art in sugarcane transformation and provide information on existing and future sugarcane improvement by genetic engineering. (frontiersin.org)
Species3
Species that cannot yet be regenerated from protoplasts are clear candidates for transformation by this method. (jrank.org)
Transformation capability will greatly facilitate gene functional studies in C. richardii , more fully realizing the potential of this model fern species. (biomedcentral.com)
Because this species produces nearly wall-less zoospores during its life cycle, synchronization was attempted using light/dark cycling and nitrate starvation to increase concentrations of zoospores to improve transformation. (wpi.edu)
Gene expression1
Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L. (scirp.org)
Stably1
Pieces of DNA may enter through these temporary pores, and sometimes protoplasts will be transformed as the new DNA is stably incorporated (i.e., able to be transmitted in mitotic cell divisions). (jrank.org)
Plasmid1
After Plasmid preparation and transformation, the amount of protoplasts expressing the GFP green fluorescent protein in the plasmid extracted with the CommaPure® kit is significantly higher than that of Company W. (biocomma.blog)
Method1
The translocation of CPPs into plant protoplasts might open up a new method for transformation of plant cells.Next, the cell-penetrating ability of the novel peptide YTA2 was characterized and it was established that chemical coupling between YTA2 and the protein cargo is not needed for the transport of the cargo over the cellular membrane in vitro. (avhandlingar.se)
Expression1
Establishment of a Protoplasts-Based Transient Expression System in Banana (Musa spp. (scirp.org)
Production1
First apparent successful production of E. oleoabundans protoplasts was achieved. (wpi.edu)
Analysis1
Instantaneous transformation analysis in petal protoplasts of P. mume revealed PmCCD4 localization in the plastid. (metabolomics.se)
System1
A versatile protoplast system and its application in Cannabis sativa L. (scirp.org)
Potential1
Transformation of Ettlia oleoabundans, a potential biofuel alga. (wpi.edu)
Application1
Three main topics on the application of CPPs have been studied in this thesis.First, several well-known CPPs, with fluorescein as a cargo, were shown to translocate into Nicotiana tabacum cultivar SR-1 protoplasts. (avhandlingar.se)
Efficient1
Efficient Isolation and Gene Transfer of Protoplast in Korean Soybean (Glycine Max (L.) Merr. (scirp.org)