• Till date, most of the studies on wheat transformation have focussed on few model cultivars and the procedures employed require a constant supply of immature embryos which is difficult to achieve throughout the year unless greenhouse grown plants are utilized. (biomedcentral.com)
  • The present work was thus initiated to develop an efficient method of transformation for Indian cultivars of bread wheat and durum wheat. (biomedcentral.com)
  • The low success rate in wheat transformation experiments thus necessiates the use of a large number of explants throughout the year which is difficult to achieve with immature embryos as the target tissue. (biomedcentral.com)
  • Therefore, the objective of the present study was to demonstrate Agrobacterium -mediated transformation of switchgrass ( Panicum virgatum L.). The A. tumefaciens strain AGL 1 carrying the binary vector pDM805, coding for the phosphinothricin acetyltransferase ( bar ) and β-glucuronidase ( gus ) genes, was utilized in these experiments. (sciencesocieties.org)
  • Calli derived from mature embryos of the cultivars CPAN1676 of T. aestivum and PDW215 of T. durum were chosen for genetic transformation experiments based on their high regeneration potential. (biomedcentral.com)
  • A comparison of the experimental transformation frequencies to literature values of the magnitude of components of the proton motive force at the same extracellular pH levels indicates that no component of the proton motive force is involved in E. coli transformation. (odec.ca)
  • paspali , there have not been any publications describing practical methods for the genetic manipulation of this fungus up till now. (tapi.com)
  • Genetic methods such as reporter gene assays and tagged mutagenesis are critical tools for unveiling the function of these genes. (mit.edu)
  • Using genetic transformation, we are able to reduce or strengthen the effects of individual genes, thereby revealing their function. (tapi.com)
  • Plastid engineering, in contrast to nuclear engineering, or conventional genetic modification, offers higher protein yields and the opportunity to express several genes controlling complex traits. (prweb.com)
  • The recent description of a method for axenic (host cell-free) propagation of C. burnetii is a significant technological advance that will facilitate biochemical and genetic studies of the pathogen ( 22 ). (asm.org)
  • Conventional methods of genetic improvement involve a recombination of pre-existing gene pools within a limited range of sexually compatible taxa. (springer.com)
  • While the conventional methods have proven remarkably successful in yielding improved genotypes that could be stabilized by back crossing, the techniques of cloning, marker-aided selection, and genetic engineering when integrated with the conventional breeding programs, will dramatically improve genetic gains. (springer.com)
  • However, due to insufficient lab equipment, and pathogenic bacteria present in the soil, we changed our focus to this topic, which can still be related to the transformation in soil. (odec.ca)
  • Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. (ejbiotechnology.info)
  • In the first section, the history and progress of Agrobacterium utilization as a transformation vector is presented along with a chapter dedicated to the analysis of the related events from a structure-functional analysis. (openaccessbooks.com)
  • The optimal pH for E. coli transformation is concluded to be about pH 7.4, supporting Norgard et al s optimal pH range of 7.25-7.75. (odec.ca)
  • The obligate intracellular nature of C. burnetii has severely impeded development of genetic tools for virulence factor discovery. (asm.org)
  • Transformation in an organization covers a vast variety of needs and activity, this is generally aimed at improving performana ce and productivity for an organization to survie. (studymode.com)