• In autologous cancer immunotherapy, T cells are extracted from the patient, genetically modified and cultured in vitro and returned to the same patient. (wikipedia.org)
  • Description of T cell growth factor interleukin-2 (IL-2) in 1976 allowed T lymphocytes to be grown in vitro, often without loss of effector functions. (wikipedia.org)
  • Southern analysis of DNA from the nonadherent cells produced by these LTC over a 6-wk period provided evidence of clonal evolution of LTC-IC in vitro. (jci.org)
  • These data demonstrate the feasibility of using cell-free virus preparations for infecting clinical marrow samples suitable for transplantation, as well as for further analysis of human marrow stem cell dynamics in vitro. (jci.org)
  • Researchers here report on in vitro experiments to show that introducing functional mitochondria into a cell culture containing senescent cells reduces markers of senescence. (fightaging.org)
  • In order to determine whether adoptive transfer of induced Treg cells generated in vitro can be used as an effective therapeutic approach to suppress airway allergic inflammation, exogenously induced Treg cells were infused into ovalbumin-sensitized mice prior to or during intranasal ovalbumin challenge. (ca.gov)
  • Therefore, adoptive transfer of in vitro induced Treg cells may be a promising therapeutic approach to prevent and treat severe asthma. (ca.gov)
  • We obtained bone marrow, isolated CD34(+) cells, performed in vitro transduction with a retroviral vector carrying a rev-responsive element (RRE) decoy gene, and reinfused the cells into these subjects with no evidence of adverse effects. (nih.gov)
  • T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
  • The primary remit of the journal is on mammalian biology both in vivo and in vitro but studies on the biochemistry and function of cells in situ are especially encouraged . (wiley.com)
  • Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
  • The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
  • Transplantation antigens serve as targets for T-cell killing in allogeneic immune responses such as in vivo graft rejection 3 and destruction of allogeneic cells by cytotoxic T cells in vitro 4 . (arizona.edu)
  • Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
  • We previously found that exosomes released from hypoxic CRC cells enhanced tumour growth and angiogenesis by enhancing the proliferation and migration of endothelial cells ( 13 ). (ijbs.com)
  • Consistent with these findings, we found that Wnt4 was enriched in exosomes released from hypoxic CRC cells and mediated the functions of endothelial cells ( 13 ). (ijbs.com)
  • Importantly, renal biopsies from two patients with vasculitis showed leukocyte-derived microvesicles bearing B1-receptors docking on glomerular endothelial cells. (lu.se)
  • Microvesicles derived from B1-receptor-transfected human embryonic kidney cells transferred B1-receptors to wild-type human embryonic kidney cells, lacking the receptor, and to glomerular endothelial cells. (lu.se)
  • Functionally, sEVs derived from GC cells infected with H. pylori deliver miR-1246 to lymphatic endothelial cells (LECs) and promote lymphangiogenesis and lymphatic remodeling . (bvsalud.org)
  • 3 Autologous lymphocytes genetically modified with the TCR for MART-1/Melan-A have been adoptively transferred to patients with advanced melanoma. (haematologica.org)
  • Results Herein we reveal a new way to reverse the tolerant state of adoptively transferred CD8 + T cells against tumors using TLR-activated B cells. (bmj.com)
  • further, stem cells have low immunogenicity because of lower levels of surface antigens . (fightaging.org)
  • Genetic modification of hematopoietic stem cells with genes that inhibit replication of human immunodeficiency virus-1 (HIV-1) could lead to development of T lymphocytes and monocytic cells resistant to HIV-1 infection after transplantation. (nih.gov)
  • They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
  • Pellets retrieved from centrifugation amniotic fluid has a rich source of stem cells. (cellbios.com)
  • Bone marrow mesenchymal stem cells (BMSCs) have potential therapeutic benefits for the treatment of endometrial diseases and injury. (biologists.com)
  • STEM CELL THERAPY IN UKRAINE AND RUSSIA Recently, there have been changes in stem cells treatment laws in Ukraine and Russia. (nbscience.com)
  • Wnt4 promotes the proliferation of cancer stem cells in response to progesterone in breast cancer ( 20 ). (ijbs.com)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • Adoptive cell transfer (ACT) is the transfer of cells into a patient. (wikipedia.org)
  • Adoptive transfer of induced-treg cells effectively attenuates murine airway allergic inflammation. (ca.gov)
  • The results showed that adoptive transfer of induced Treg cells prior to allergen challenge markedly reduced airway hyperresponsiveness, eosinophil recruitment, mucus hyper-production, airway remodeling, and IgE levels. (ca.gov)
  • Moreover, adoptive transfer of induced Treg cells during allergen challenge also effectively attenuate airway inflammation and improve airway function, which are comparable to those by natural Treg cell infusion. (ca.gov)
  • The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)
  • The data support the broad value of CD20 as safety switch in adoptive T-cell therapy. (haematologica.org)
  • In this study, we investigated the use of CD20 as safety switch after retroviral transfer to T cells with different cytomegalovirus (CMV) specificities, and conclude that CD20 may be broadly applicable as safeguard in adoptive T-cell therapy. (haematologica.org)
  • None of the iMC from the different tumor models suppressed CTL responses in adoptive cell transfer experiments, unless GM-CSF was provided in vivo. (mdc-berlin.de)
  • Adoptive transfer of activated autologous tumor-infiltrating lymphocytes (TIL) can mediate complete, durable regressions in patients with metastatic melanoma. (aacrjournals.org)
  • Background Adoptive T cell transfer (ACT) therapy improves outcomes in patients with advanced malignancies, yet many individuals relapse due to the infusion of T cells with poor function or persistence. (bmj.com)
  • Retroviral gene transfer to primitive normal and leukemic hematopoietic cells using clinically applicable procedures. (jci.org)
  • Clinical uses of gene transfer to bone marrow transplants require the establishment of a reproducible method for infecting large numbers of very primitive hematopoietic cells at high efficiency using cell-free retrovirus-containing media. (jci.org)
  • Marrow cells enriched greater than or equal to 500-fold for LTC-IC (1-2% pure) by flow cytometry showed gene transfer efficiencies of 27% when infected in a similar fashion over a shorter period (24 h), but in the presence of added soluble IL-3 and G-CSF without stromal feeders, and this increased to 61% when Steel factor was also present during the infection period. (jci.org)
  • By using a less highly enriched population of LTC-IC obtained by a bulk immunoselection technique applicable to large-scale clinical marrow harvests, gene transfer efficiencies to LTC-IC of 40% were achieved and this was increased to 60% by short-term preselection in G418. (jci.org)
  • The levels of gene-containing leukocytes in peripheral blood samples in the 1 year after gene transfer/cell infusion have been extremely low. (nih.gov)
  • These observations support the potential of performing gene therapy for HIV-1 using hematopoietic cells, but emphasize the need for improved gene transfer techniques. (nih.gov)
  • What causes gene transfer to trigger T cell activation and exhaustion? (medicalxpress.com)
  • A new review that takes a comprehensive look at both the AAV-mediated immune reactivation response to gene transfer and the role that regulatory and exhausted T cells play in the development of tolerance to AAV is published in Human Gene Therapy . (medicalxpress.com)
  • Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). (uni-bielefeld.de)
  • Pulsing conditions inducing reversible permeabilization of the cell plasma membrane are not sufficient to induce gene transfer. (uni-bielefeld.de)
  • Wolf H, Rols MP, Boldt E, Neumann E, Teissie J. Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. (uni-bielefeld.de)
  • Control by pulse parameters of electric field-mediated gene transfer in mammalian cells", Biophysical Journal , vol. 66, 1994, pp. 524-531. (uni-bielefeld.de)
  • However, even when the two organisms in question are in close proximity to each other, such as in a symbiotic or parasitic relationship, the transfer of genetic material and its introduction into a new genome only marks the initial step for successful horizontal gene transfer. (phys.org)
  • In a new discovery that increases our understanding of gene transfer, a research team centered at University of Tsukuba has studied a gene in marine invertebrates called ascidians originally came from a common bacterium. (phys.org)
  • This epidemic expansion probably resulted from a single source after a lateral gene transfer (LGT) event that changed the serotype of an epidemic V. cholerae O1 El Tor strain to O139. (cdc.gov)
  • This new serotype appears to have resulted when a lateral gene transfer (LGT) event occurred that replaced the 22 kb of the wbf region (encoding the O1 antigen) of a seventh pandemic V. cholerae O1 El Tor strain with a 37-kb region encoding the O139 polysaccharide ( 3 - 5 ). (cdc.gov)
  • Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • Here we describe transient protein production using QMCF Technology (Icosagen, Estonia) that uses specific suspension-adapted mammalian cell lines (QMCF cells), appropriate QMCF episomal expression vector, a chemically defined animal origin-free, serum-free medium, and Reagent 007™ (Icosagen, Estonia) for efficient delivery of nucleic acids for transfection of mammalian cells in comparison with PEI MAX™ (Transfection Grade Linear Polyethylenimine Hydrochloride, Polysciences). (springer.com)
  • Mammalian cells of different species differ in sensitivity to ouabain. (rupress.org)
  • New vector for transfer of yeast artificial chromosomes to mammalian cells. (ox.ac.uk)
  • A modification vector has been constructed to facilitate the transfer of yeast artificial chromosomes (YACs) to mammalian cells in culture by targeting a dominant selectable marker (G418 resistance) to the right arm of pYAC4 clones. (ox.ac.uk)
  • Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
  • The researchers used a standard method of density gradient to isolate mononuclear cells [NK Cells]. (agelessway.org)
  • Syngeneic lymphocytes were transferred from rodents heavily immunized against the tumor to inhibit growth of small established tumors, becoming the first example of ACT. (wikipedia.org)
  • In 2002, lymphodepletion using a nonmyeloablative chemotherapy regimen administered immediately before TIL transfer increased cancer regression, as well as the persistent oligoclonal repopulation of the host with the transferred lymphocytes. (wikipedia.org)
  • In 2006 administration of normal circulating lymphocytes transduced with a retrovirus encoding a T-cell receptor (TCR) that recognized the MART-1 melanoma-melanocyte antigen, mediated tumor regression. (wikipedia.org)
  • In 2010 administration of lymphocytes genetically engineered to express a chimeric antibody receptor (CAR) against B cell antigen CD19 was shown to mediate regression of an advanced B cell lymphoma. (wikipedia.org)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • A recloned HEB cybrid (HEB7A) has also been enucleated and fused to hypoxanthine phosphoribosyl transferase (HPRT)-deficient HeLa cells (S3AG1) and HPRT-deficient lymphocytes (WAL-2A). (rupress.org)
  • Methods In this study we investigated how tumor-specific murine CD8 + T cells and human tumor infiltrating lymphocytes (TILs) are impacted when expanded ex vivo with the TLR9 agonist CpG. (bmj.com)
  • Recently, we have demonstrated that both L d (ref. 8) and K d molecules (unpublished data) expressed on transformed L cells can act as target antigens for alloreactive cytotoxic T lymphocytes. (arizona.edu)
  • In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
  • In this review, we summarized the mechanism of mitochondrial transfer in the cardiovascular system and outlined the fate and functional role of donor mitochondria. (frontiersin.org)
  • BMSCs interact with uterus parenchymal cells by direct contact or indirect secretion of growth factors to promote functional recovery. (biologists.com)
  • Here we determined whether leukocyte-derived microvesicles bear B1-kinin receptors during vasculitis, and if microvesicles transfer functional B1-receptors to recipient cells, thus promoting inflammation. (lu.se)
  • Thus, we found a novel mechanism by which microvesicles transfer functional receptors and promote kinin-associated inflammation. (lu.se)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • In 2017, researchers announced the first use of donor cells (rather than the patients' own cells) to defeat leukemia in two infants for whom other treatments had failed. (wikipedia.org)
  • Given the complexity of the biological function of mitochondria, researchers have begun to consider rescuing the injured cells through mitochondrial transfer, that is, replacing damaged mitochondria with healthy mitochondria from donor cells. (frontiersin.org)
  • Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
  • Most of these efforts are focused on donor cells. (biomedcentral.com)
  • Comparatively, allogeneic therapies involve cells isolated and expanded from a donor separate from the patient receiving the cells. (wikipedia.org)
  • Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
  • 4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
  • T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)
  • The Bone Marrow Collection Kit is used to remove the bone, chips, fat tissues and filter aspirated bone marrow for autologous and allogeneic stem cell transplantation. (cellbios.com)
  • Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • In some patients, the administered antitumor cells represented up to 80% of the CD8+ T cells months after the infusion. (wikipedia.org)
  • It is an interesting question as to how this would work in living tissue, where the numbers of senescent cells are low, and mitochondria will be introduced into all cells. (fightaging.org)
  • Those groups are not specifically targeting cellular senescence, but can hardly avoid having senescent cells taking up their therapeutic mitochondria. (fightaging.org)
  • Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)
  • Studies have shown that cells can eliminate defective mitochondria by delivering them to recipient cells (such as macrophages) to maintain homeostasis. (frontiersin.org)
  • And the released mitochondria can also act as a distress signal to activate the rescue properties of recipient cells ( 12 , 13 ). (frontiersin.org)
  • However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
  • We investigated the effects of Cd on the individual complexes of the electron transfer chain (ETC) and on the stimulation of reactive oxygen species (ROS) production in mitochondria. (cdc.gov)
  • The semiubiquinones, being unstable, are prone to transfer one electron to molecular oxygen to form superoxide, providing a possible mechanism for Cd-induced generation of ROS in mitochondria. (cdc.gov)
  • In 2016, CD19-specific chimeric antigen receptor (CAR)-modified T cells were used to treat patients with relapsed and refractory CD19+ B cell malignancies, including B cell acute lymphoblastic leukemia (B-ALL) harboring rearrangement of the mixed lineage leukemia (MLL) gene with CD19 CAR-T cells. (wikipedia.org)
  • The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
  • CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
  • Toll-like receptor (TLR) agonists can invigorate antitumor T cell responses when administered directly to patients, but these responses often coincide with toxicities. (bmj.com)
  • Thus the T-cell receptor recognizes the viral antigen in the context of a class I molecule. (arizona.edu)
  • To study the interaction between the T-cell receptor and the class I restricting element, we have used the mouse L-cell transformant 8-5 which expresses L d molecules 7 and the K7-65 transformant expressing K molecules (R.S.G. et al. (arizona.edu)
  • We focus here on the methodology insights of new techniques, such as those based on resonance energy transfer for the analysis of chemokine receptor conformations in living cells. (nih.gov)
  • The transferred B1-receptors induced calcium influx after B1-receptor agonist stimulation: a response abrogated by a specific B1-receptor antagonist. (lu.se)
  • Administration of IL-2 after cell transfer enhanced therapeutic potential. (wikipedia.org)
  • Mitochondrial transplantation into damaged cells or injured tissues is considered a novel cell-based therapeutic strategy. (fightaging.org)
  • In 1986, human TILs from resected melanomas were found to contain cells that could recognize autologous tumors. (wikipedia.org)
  • The researchers demonstrated the method by attaching trastuzumab, an antibody used for cancer treatment, to the surface of natural killer (NK) immune cells. (acs.org)
  • Since several companies are developing mitochondrial transfer as a therapy to treat the loss of mitochondrial function that is characteristic of age-related disease, we'll find out in the years ahead. (fightaging.org)
  • Senolytics that destroy senescent cells seem a safer proposal, and efficient senolytics may turn out to be required in advance of some of the other rejuvenation therapies on the horizon, such as partial reprogramming and mitochondrial transfer. (fightaging.org)
  • The phenomenon of intercellular mitochondrial transfer has been discovered in the cardiovascular system. (frontiersin.org)
  • Studies have shown that cell-to-cell mitochondrial transfer plays an essential role in regulating cardiovascular system development and maintaining normal tissue homeostasis under physiological conditions. (frontiersin.org)
  • We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
  • The intercellular mitochondrial transfer was reported for the first time by Spees and colleagues in 2006. (frontiersin.org)
  • can act as donors or recipients during mitochondrial transfer under physiological conditions ( 8 - 12 ). (frontiersin.org)
  • However, harmful stimuli (such as ischemia-reperfusion, oxidative stress, and toxic chemicals) can change the direction and efficiency of intercellular mitochondrial transfer. (frontiersin.org)
  • Attempts to use T cells to treat transplanted murine tumors required cultivating and manipulating T cells in culture. (wikipedia.org)
  • Tumors frequently induce immature myeloid cells (iMC), which suppress specific and unrelated cytotoxic T lymphocyte (CTL) responses and are termed myeloid derived suppressor cells (MDSC). (mdc-berlin.de)
  • Liu X, Li S, Zhang X. An experimental study on the malignant characteristics of gemcitabine resistant cells by Transferring MiRNA-222-3p via exosomes. (minervamedica.it)
  • Cancer cells release exosomes with various functions into the tumour microenvironment during cancer progression. (ijbs.com)
  • However, the roles and associated mechanisms of hypoxic colorectal cancer (CRC) cell-derived exosomes remain poorly understood. (ijbs.com)
  • Here, we found that exosomes secreted by hypoxic CRC cells promoted the migration and invasion abilities of normoxic CRC cells. (ijbs.com)
  • Taken together, the results of our study indicate that hypoxia may stimulate tumour cells to release Wnt4-rich exosomes that are delivered to normoxic cells to enhance prometastatic behaviours, which might provide new targets for CRC treatment. (ijbs.com)
  • As previously reported, colorectal cancer cell-derived exosomes have important roles in tumour progression including invasion, angiogenesis, immune modulation and distal metastasis through effectively delivering microRNAs, mRNAs and proteins ( 10 - 12 ). (ijbs.com)
  • However, the functions and underlying molecular mechanisms of hypoxic CRC cell-derived exosomes are still largely unknown. (ijbs.com)
  • In this study, we sought to identify new functions of hypoxic CRC cell-derived exosomes. (ijbs.com)
  • We found that exosomes released from hypoxic CRC cells enhanced the migration and invasion abilities of normoxic CRC cells. (ijbs.com)
  • The transfer of genes from one organism to another is potentially a rapid way for evolution to occur and for complicated novel functions to emerge. (phys.org)
  • The major histocompatibility complex (MHC) of the mouse, H-2 on chromosome 17, contains several families of genes encoding cell-surface molecules which have a role in mediating immune responses 1,2 . (arizona.edu)
  • Using 86Rb+ as a measure of intracellular K+, we found higher levels of radioactivity in mixtures of ouabain-sensitive and -resistant cells cultured in the presence of ouabain than predicted from pure cultures of the two component cell types. (rupress.org)
  • Coculture of ouabain-sensitive and ouabain-resistant cells in the presence of ouabain also leads to restoration of the capacity for protein synthesis. (rupress.org)
  • Autoradiography shows that this restoration occurs in the sensitive cell type and is dependent upon contact with ouabain-resistant cells. (rupress.org)
  • Furthermore, sensitive cells are able to multiply in the presence of ouabain when cocultured with resistant cells. (rupress.org)
  • In 2016, researchers developed a technique that used cancer cells' RNA to produce T cells and an immune response. (wikipedia.org)
  • We show that the dynamic changes occurring in BRAF V600E ctDNA levels within the first month following T-cell transfer immunotherapy in metastatic melanoma can be used to rapidly identify responding from nonresponding patients, potentially allowing clinicians to make critical treatment-related decisions in a more timely manner. (aacrjournals.org)
  • The use of live cells as therapies could become more widespread if there were easy ways to modify cell surfaces. (acs.org)
  • Conclusions Our results demonstrate a novel way to use TLR agonists to improve immunotherapy and reveal a vital role for B cells in the generation of potent CD8 + T cell-based therapies. (bmj.com)
  • The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies. (mssm.edu)
  • When you study Cell and Molecular Biology (CMB) you will focus on understanding cellular development and function and how cell function affects the whole living organism. (grcc.edu)
  • The Cell and Molecular Biology pre-major is for academically motivated students who have a strong interest in the life sciences and plan to transfer the four-year college or university of their choice to complete a Bachelor of Science in CMB. (grcc.edu)
  • Therefore, it can be concluded that CAP resistance is coded in the cytoplasm and not in the nucleus of 296-1 cells. (rupress.org)
  • Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
  • Moreover, exosomal Wnt4 enhanced β-catenin translocation to the nucleus in normoxic CRC cells. (ijbs.com)
  • The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
  • In order to increase the power conversion efficiency of organic solar cells, their absorption spectrum should be broadened while maintaining efficient exciton harvesting. (nature.com)
  • The ITO/sMWCNT/CSI/CdS electrode exhibit an incident photon to photocurrent efficiency (IPCE) of 7% at an applied bias of +0.2 V vs. SCE in a standard three-compartment electrochemical cell . (rsc.org)
  • Various strategies have been used to improve the efficiency of nuclear transfer, however, significant breakthroughs are yet to happen. (biomedcentral.com)
  • Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
  • Next, they introduced 30 thousand K-562 tumor cells (erythroblastic human leukemia) into the wells. (agelessway.org)
  • In the control series of the experiments for all of the transfer factor samples tested, there was no direct cytotoxic effect on tumor cells at any of the concentrations tested. (agelessway.org)
  • When in the absence of the immune NK cells, they add the transfer factors directly with the cancer cells there is no cytotoxic effect on the tumor cells. (agelessway.org)
  • Trastuzumab-NK conjugates are more effective than unmodified NK cells in killing cancer cells both in cell culture and in mice. (acs.org)
  • The isolated cells, 60 thousand cells per well, in 100 ml of culture medium, were introduced into each well of Costar 96 well plates. (agelessway.org)
  • The simplest explanation is that K+ and 86Rb+ are being transferred from ouabain-resistant to ouabain-sensitive cells, enhancing the total intracellular 86Rb+ in the culture. (rupress.org)
  • A function, "index of cooperation," expresses this enhancement as a number ranging from 0 to 1, and permits comparisons to be made under various culture conditions and using various cell types. (rupress.org)
  • An index of cooperation greater than 0 requires cell contact, since no enhancement occurs when contact between two cell types in the same culture is prevented. (rupress.org)
  • Cytoplasmic transfer of chloramphenicol resistance in human tissue culture cells. (rupress.org)
  • Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC³s). (uni-bielefeld.de)
  • T cells expanded ex vivo from a CpG-treated culture demonstrated potent antitumor efficacy and prolonged persistence in vivo. (bmj.com)
  • CpG-conditioned CD8 + T cells acquired a unique proteomic signature hallmarked by an IL-2Rα high ICOS high CD39 low phenotype and an altered metabolic profile, all reliant on B cells transiently present in the culture. (bmj.com)
  • Isolated B cells also imparted T cells with the CpG-associated phenotype and improved tumor immunity without the aid of additional antigen-presenting cells or other immune cells in the culture. (bmj.com)
  • The CellStor® EVA Transfer Bags of lower volumes 5mL To 5L achieve many clinical applications includes, hormone preparations for cell culture reagent bags for sensitive chemo drugs like Chloroambucil, Nimotuzumab, etc. (cellbios.com)
  • protein patterns of subjects with leish- Cell culture maniasis [17]. (who.int)
  • Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
  • Small Extracellular Vesicles Derived from Helicobacter Pylori-Infected Gastric Cancer Cells Induce Lymphangiogenesis and Lymphatic Remodeling via Transfer of miR-1246. (bvsalud.org)
  • Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
  • Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
  • The use of the technique of nuclear transfer for reproduction of human beings is surrounded by strong ethical concerns and controversies and is considered a threat to human dignity. (who.int)
  • 3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
  • In checkpoint therapy, antibodies bind to molecules involved in T-cell regulation to remove inhibitory pathways that block T-cell responses, known as immune checkpoint therapy. (wikipedia.org)
  • To prevent the reactions CellBios designed CellStor EVA Transfer Bags for effective processing and storage of compounds and others highly reactive pharmaceutical Molecules. (cellbios.com)
  • Mouse L cells are fibroblasts derived from C3H mice of H-2 k haplotype and monoclonal antibodies can be used to distinguish H-2 d molecules from the endogenous H-2 k products. (arizona.edu)
  • The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
  • Fundamental studies of photoelectrochemical processes in complex materials, including photophysics and photochemistry of light-harvesting and light-emitting molecules, as well as energy and electron transfer processes in supramolecular systems and molecular-semiconductor hybrid nanomaterials. (lu.se)
  • Exogenous mitochondrial transplantation improved mitochondrial dysfunction and alleviated cellular senescence hallmarks, such as increased cell size, increased senescence-associated β-galactosidase activity, augmented NF-κB activity, increased inflammatory cytokines , and upregulated the cyclin-dependent kinase inhibitors p21 and p16 . (fightaging.org)
  • Further, cellular senescence properties were improved by exogenous mitochondrial transplantation in oxidative stress -induced senescent ARPE-19 cells. (fightaging.org)
  • Notwithstanding the centuries we've spent peering at, poking, prodding and penetrating the inner workings of plant cells with various types of microscopes and decades undertaking investigations at the sub-cellular. (botany.one)
  • CellStor® EVA Transfer Bags are used for the collection, processing, and storage of blood & blood components, cell & cellular components, etc. (cellbios.com)
  • Cell Biochemistry & Function publishes original research articles and reviews on the mechanisms whereby molecular and biochemical processes control cellular activity with a particular emphasis on the integration of molecular and cell biology, biochemistry and physiology in the regulation of tissue function in health and disease . (wiley.com)
  • Microvesicles derived from neutrophils also transferred B1-receptors to wild-type human embryonic kidney cells and induced calcium influx after stimulation. (lu.se)
  • The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)
  • The cells had four genetic modifications. (wikipedia.org)
  • These studies represent the first genetic evidence of cytoplasmic inheritance in human cells. (rupress.org)
  • if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
  • In the article entitled, " Regulatory and Exhausted T Cell Responses to AAV Capsid ," the researchers discuss the complexity of the immune response to AAV, the broad variability in responses between individual patients exposed to AAV vectors, strategies in development to suppress an immune reaction to viral vector components, and the implications of this evolving research area to future developments in AAV-based gene therapy. (medicalxpress.com)
  • Gwladys Gernoux et al, Regulatory and Exhausted T Cell Responses to AAV Capsid, Human Gene Therapy (2017). (medicalxpress.com)
  • Co-transfer of a suicide gene would provide a desirable safety switch in clinical TCR gene therapy. (haematologica.org)
  • In parallel, the development of Cell Therapy products is a rapidly expanding market and also an opportunity for the application of microfluidic flow control. (knowledgetransferireland.com)
  • CellStor EVA Transfer Bags are constructed of single-layer film optimized for the protection of high-value biopharmaceuticals, biologics, blood & blood components, cell and gene therapy products & intermediates as well as in-process and finished products from the bioprocess industry. (cellbios.com)
  • In vivo, this electrical charge guided the particles towards the patient's dendritic immune cells that specify immune system targets. (wikipedia.org)
  • Defects in endogenous Treg cells have been reported in patients with allergic asthma, suggesting that disrupted Treg cellmediated immunological regulation may play an important role in airway allergic inflammation. (ca.gov)
  • This effect was associated with increase of Treg cells (CD4+FoxP3+) and decrease of dendritic cells in the draining lymph nodes, and with reduction of Th1, Th2, and Th17 cell response as compared to the controls. (ca.gov)
  • The Treg and exhausted T cell responses that this article discusses may be the key to explaining this property, which appears to be critical for the clinical success of AAV as a vector," says Editor-in-Chief Terence R. Flotte, MD, Celia and Isaac Haidak Professor of Medical Education and Dean, Provost, and Executive Deputy Chancellor, University of Massachusetts Medical School, Worcester, MA. (medicalxpress.com)
  • Ed. - And, if your appetite for TCs has now been whetted, these curious cells will feature in a future Research Topic in Frontiers of Plant Physiology to be edited by David McCurdy and Gregorio Hueros. (botany.one)
  • Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
  • Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
  • Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
  • We posited that TLR agonists could be repurposed ex vivo to condition T cells with remarkable potency in vivo, circumventing TLR-related toxicity. (bmj.com)
  • 1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
  • Inhibition of exosome secretion by GW4869 reduced hypoxic exosome-mediated migration and invasion of normoxic CRC cells. (ijbs.com)
  • Karro K, Männik T, Männik A, Ustav M (2015) DNA transfer into animal cells using stearylated CPP based transfection reagent. (springer.com)
  • Longo PA, Kavran JM, Kim M-S, Leahy DJ (2013) Transient mammalian cell transfection with polyethylenimine (PEI). (springer.com)
  • Combined Numerical and Experimental Investigation of Localized Electroporation-Based Cell Transfection and Sampling. (uni-bielefeld.de)
  • Electron transfer at the cell-uranium interface in Geobacter spp. (portlandpress.com)
  • In these nanohybrid materials photoinduced electron transfer phenomena are found to occur from the photoexcited CdS nanoparticles to the nanotubes as evidenced by the efficient fluorescence emission quenching of CdS nanoparticles . (rsc.org)
  • Cadmium inhibits the electron transfer chain and induces reactive oxygen species. (cdc.gov)
  • Potential effects of forced CD20 expression on T-cell function were investigated by comparing CD20- and mock-transduced cytomegalovirus (CMV) specific T cells for cytolysis, cytokine release and proliferation. (haematologica.org)
  • they combined NK cells from humans with cancer cells and divided them into groups of transfer factors activated NK cells and groups of inactivated NK cells. (agelessway.org)
  • CpG fostered the expansion of potent CD8 + T cells with the signature phenotype and antitumor ability via empowering a direct B-T cell interaction. (bmj.com)
  • This process depends on the endo-lysosomal sterol transfer protein Niemann Pick C2 (NPC2). (nature.com)
  • This sensitivity is expressed as reduced intracellular K+ content, reduced rates of protein synthesis, and cessation of cell multiplication. (rupress.org)
  • Thus K+, presumably transferred to sensitive cells through gap junctions, is able to counteract the toxic effects of ouabain on intracellular K+ levels and protein synthesis, and to restore growth. (rupress.org)
  • A bacterial fucosyltransferase can add antibodies to cell surfaces in a one-step reaction. (acs.org)
  • The characteristics of this response would include production of neutralizing antibodies, generation of a T-cell response, and avoidance of immune-enhanced disease4. (who.int)
  • they are 'specialized parenchyma cells that have an increased surface area, due to infoldings of the plasma membrane. (botany.one)
  • Those plasma membrane infoldings are the result of cell wall in growths and transfer cells (TCs) appear to have been present in angiosperms for over 50 million years . (botany.one)
  • These results confirm that multilayer cascade structures are a promising alternative to conventional donor-fullerene organic solar cells. (nature.com)
  • Organic solar cells are a potential low-cost alternative to conventional inorganic solar cells due to their ease of processing and compatibility with flexible substrates 1 , 2 . (nature.com)
  • In this work, the analysis of the mass transfer phenomena in catalytic open-cell foams is carried out through the combination of computational fluid dynamics (CFD) simulations and experiments, using the CO oxidation on Pt(1%)/γ-Al 2 O 3 /foam as a model reaction. (tue.nl)