MembranesBilayerPhospholipidsMetabolismPhosphatidylinositolSignal transductionMoleculesKinasePhosphatidylethanolamineVesiclesSynthesisInositol lipidPhospholipid TransferBindsEndoplasmic reticulumLiverApolipoproteinsCellularCholesteryl estersHydrophobicBilayersSTRUCTURESTriglyceridesCytosolic proteinsSolublePeripheralCholesterolPeptidesBiophysicalRecombinantCytosolAnionicPathwaysSterolsDropletsNeuronalStarD2ModulateSubfamilySpectroscopyTransporterBileInhibitionTransmembraneTissuesAbnormalitiesFamily proteinsChemicalInteractionsSpeciesCholineResiduePhosphorylationBindRisk of atherosclerosisMRNABoundKineticsLipoproteinIntestinalDisordersAtherosclerosisCytoskeletalTransportMicroscopyPctp
Membranes18
- Phosphatidylcholine transfer protein (PCTP), also known as StAR-related lipid transfer domain protein 2 (STARD2), is a specific intracellular phospholipid binding protein that can transfer phosphatidylcholine between different membranes in the cytosol. (wikipedia.org)
- PCTP transfers phosphatidylcholine molecules between membranes in vitro. (wikipedia.org)
- STARD10 is thought to function as a dual specificity lipid transfer protein capable of shuttling phosphatidylcholine and phosphatidylethanolamine between membranes. (reactome.org)
- Proteins which are found in membranes including cellular and intracellular membranes. (lookformedical.com)
- A procedure was developed to vary the cholesterol-to-phospholipid (Ch/PL) ratio of synaptic plasma membranes and synaptosomes using a nonspecific lipid transfer protein so that membrane lipid composition could be correlated with presynaptic function. (mcw.edu)
- In synaptic plasma membranes, Ch/PL molar ratios from 0.21 to 1.19 were produced from a normal value of 0.52 +/- 0.01 by incubation with the transfer protein and an excess of either phosphatidylcholine or cholesterol/phosphatidylcholine liposomes for 60 min at 32 degrees C. In synaptosomes, Ch/PL ratios from 0.16 to 0.81 were similarly produced from a normal value of 0.38 +/- 0.04. (mcw.edu)
- Cholesterol loading or depletion of the membranes was accompanied by a decrease or increase, respectively in the phospholipid-to-protein ratio. (mcw.edu)
- Lipid transport proteins at membrane contacts, where organelles are closely apposed, are critical in redistributing lipids from the endoplasmic reticulum (ER), where they are made, to other cellular membranes. (rupress.org)
- Phosphatidylinositol transfer proteins (PITPs) can bind specifically and transfer a single phosphatidylinositol (PI) molecule between phospholipid membranes in an ATP-independent manner in vitro. (embl.de)
- Before taking effect, pharmaceuticals that target cell function must cross a crucial barrier: the complex assembly of lipids, proteins, and carbohydrates that make up biological membranes. (rdworldonline.com)
- We think that insights from this study can lead to better understanding and predictions of protein structure as well as electron transfer processes within cellular membranes," Tahara concludes. (rdworldonline.com)
- Current work is directed towards the study of protein-membrane interactions over all timescales and the preparation of asymmetric lipid membranes for biophysical studies of membrane activity (funded by an EPSRC programme grant). (dur.ac.uk)
- Work encompasses the design of peptides that have the potential to form stable pores in lipid membranes, the characterisation of peptide interactions with membrane lipids and the physical organic chemistry of peptide reactions in membranes. (dur.ac.uk)
- The behaviour of membrane proteins in membranes is being examined using a range of analytical approaches. (dur.ac.uk)
- Current work is examining the chemistry exhibited by drug molecules after their adsorbtion to lipid membranes. (dur.ac.uk)
- Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ. (mpg.de)
- Distance measurements in membranes using spin label saturation transfer electron-spin-resonance. (mpg.de)
- The recruitment of specific cytosolic proteins to intracellular membranes through binding phosphorylated derivatives of phosphatidylinositol (PtdIns) controls such processes as endocytosis, regulated exocytosis, cytoskeletal organization, and cell signaling. (embl.de)
Bilayer9
- These differences can produce significant variations in membrane physical properties, in the location of phospholipids in a lipid bilayer and in their biological activity. (thermofisher.com)
- The attachment position of the fluorophore determines whether it is located in the nonpolar interior or at the water/lipid interface when the phospholipid analog is incorporated into a lipid bilayer membrane ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
- Integration of a K+ channel-associated peptide in a lipid bilayer: Conformation, lipid-protein interactions, and rotational diffusion. (mpg.de)
- The transport domains are mobile and move through the lipid bilayer (alike an elevator) when translocating the amino acid substrate and co-transported ions across the membrane 13 . (nature.com)
- The structures show that both substrate translocation and release involve movements of the bulky transport domain through the lipid bilayer. (elifesciences.org)
- Finally, we describe how domain movements are associated with the displacement of bound lipids and significant membrane deformations, highlighting the potential regulatory role of the bilayer. (elifesciences.org)
- The two hairpins meet near the middle of the lipid bilayer, and their non-helical tips provide essential coordinating moieties for the bound L-asp. (elifesciences.org)
- These EV contain a lipid bilayer associated to membrane proteins. (frontiersin.org)
- The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)
Phospholipids9
- Membrane probes include fluorescent analogs of natural lipids, as well as lipophilic organic dyes that have little structural resemblance to natural biomolecules ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
- We offer fluorescent and, in a few cases, biotinylated analogs of five naturally occurring lipid classes-phospholipids, sphingolipids (including ceramides), fatty acids, triglycerides and steroids. (thermofisher.com)
- Fatty acids are liberated by the enzymatic action of phospholipase A on phospholipids ( Probes for Lipid Metabolism and Signaling-Section 17.4 ) and also by various other lipases. (thermofisher.com)
- Certain fluorescent fatty acids ( Fatty Acid Analogs and Phospholipids-Section 13.2 ) are readily metabolized by live cells to phospholipids, mono-, di- and triacylglycerols, cholesteryl esters and other lipid derivatives. (thermofisher.com)
- Phospholipids include phosphatidylcholine (PC), sphingomyelin (SM), inositol lipid (PI), and lysophosphatidyl choline (LPC), among which LPC is the main component of oxidized low density lipoprotein (LDL) and degrades production of PC with its cytotoxic effect [ 10 ]. (hindawi.com)
- PI transfer protein: the specific recognition of phospholipids and its functions. (embl.de)
- Some Arabidopsis ACBPs have also been observed to bind phospholipids in filter binding assays, e.g. recombinant ACBP6 binds phosphatidylcholine (PC). (aocs.org)
- Enzymatic hydrolysis of membrane phospholipids by phospholipases produces these lipid second messengers, as well as soluble second messengers such as inositol 1,4,5-trisphosphate, and also other molecules such as choline (Cho) that are released to the extracellular medium. (jneurosci.org)
- The nonpolar lipids (ie, cholesterol ester, triglycerides [TGs]) reside in a core surrounded by more polar components (eg, free cholesterol, phospholipids, proteins). (medscape.com)
Metabolism18
- The protein is expressed at high levels in tissues engaged in high metabolism, notably including the liver and macrophages. (wikipedia.org)
- PCTP-like protein (STARD10) is a member of the steroidogenic acute regulatory (StAR) protein-related lipid transfer (START) domain proteins that are implicated in lipid transport, metabolism, and signaling. (reactome.org)
- The aim of this study was to evaluate the beneficial effects and potential mechanisms of genistein (GEN) on production performance impairments and lipid metabolism disorders in laying hens fed a high-energy and low-protein (HELP) diet. (bvsalud.org)
- Fatty liver hemorrhagic syndrome (FLHS) is a nutritional and metabolic disease that seriously threatens the health and performance of laying hens, which is characterized by hepatic steatosis and lipid metabolism disorders. (bvsalud.org)
- As an isoflavone phytoestrogen, genistein (GEN) exerts many beneficial functions, including alleviating lipid metabolism disorders and anti-inflammatory properties. (bvsalud.org)
- Moreover, GEN treatment alleviated hepatic steatosis and lipid metabolism disorders through reducing the expression levels of mRNA related to fatty acid transport and synthesis and enhancing the mRNA expression levels of factors associated with fatty acid oxidation in FLHS layers, which may be achieved by activation of the G protein-coupled estrogen receptor-adenosine 5'-monophosphate (AMP)-activated protein kinase signaling pathways. (bvsalud.org)
- Kinetically down-regulated genes involved in the lipid metabolism are: hydroxyacid oxidase 3, fatty acid CoA ligase long chain 2, and steroyl CoA desaturase 1.The regulation of protein expression of the cell cycle genes CDC42 and cyclin D1 was studied by Western Blot technique, in the oval cell liver regeneration. (uni-goettingen.de)
- One of those molecules, phosphatidylcholine transfer protein (PCTP), suppresses the activity of the other, PPARdelta, a gene regulator critical in burning fat and energy metabolism, under conditions of overnutrition. (anl.gov)
- To deliver their functions, some of them need to be transported within and across subcellular compartments during lipid metabolism. (aocs.org)
- These observations on the distribution of ACBPs to a variety of locations are not unexpected if ACBPs were to cater for different functions in plant lipid metabolism. (aocs.org)
- Intestinal microbial metabolism of phosphatidylcholine and cardiovascular risk. (appyuntamiento.es)
- d) It is highly significant in the liver and kid-neys of birds which excrete uric acid as the end product of purine metabolism and also of protein and amino acid catabolism. (biologydiscussion.com)
- Perilipin 5 S155 phosphorylation by PKA is required for the control of hepatic lipid metabolism and glycemic control. (uci.edu)
- Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
- We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism. (uci.edu)
- Re-expression of PLIN5 S155A in the liver of Plin5 liver-specific null mice reduced lipolysis compared with wild-type PLIN5 re-expression, but was not associated with other changes in hepatic lipid metabolism. (uci.edu)
- The proteins, termed apolipoproteins, play an important role in lipoprotein metabolism. (medscape.com)
- Together, our findings provide new insights into molecular changes associated with metabolic risk conditions, suggest new condition-specific associations between apolipoproteins and lipids, and may inform new biomarker discovery in lipid metabolism-associated disorders. (cdc.gov)
Phosphatidylinositol5
- They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins. (lookformedical.com)
- Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p) and in RhoGAPs, RhoGEFs and the RasGAP, neurofibromin (NF1). (embl.de)
- The phosphorylation of phosphatidylinositol (PtdIns) on the 3' position of the inositol ring by phosphoinositide 3-kinase (PI 3-kinase) is shown to depend strongly on the curvature of liposomes containing a mixture of phosphatidylcholine (PtdCho) and PtdIns. (tamu.edu)
- Exposure of cortical neurons to neurotoxic concentrations of NMDA increased extracellular choline and activated hydrolysis of phosphatidylcholine and phosphatidylinositol by phospholipase A 2 but did not induce significant degradation of phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, or phosphatidylserine. (jneurosci.org)
- Phosphatidylinositol phosphatidylcholine transfer. (ntu.edu.sg)
Signal transduction4
- and for investigating synaptosome recycling ( Probes for Following Receptor Binding and Phagocytosis-Section 16.1 ) and lipid-mediated signal transduction processes ( Probes for Signal Transduction-Chapter 17 ). (thermofisher.com)
- Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. (lookformedical.com)
- It binds CHOLESTEROL and is involved in LIPIDS transport, membrane traffic, and SIGNAL TRANSDUCTION. (lookformedical.com)
- Phosphoinositide phosphatases play an essential but as yet not well-understood role in lipid-based signal transduction. (embl.de)
Molecules3
- Their research shows that phosphatidylcholines can act as signaling molecules and that PPARdelta can be targeted indirectly, by inhibiting PCTP. (anl.gov)
- Tahara and colleagues used their HD-VSFG technique to make only surface water molecules vibrate at a phosphatidylcholine interface. (rdworldonline.com)
- It involves the use of a high-energy spray of charged solvent droplets that desorb and ionize molecules from the sample, which are then transferred into a mass spectrometer for analysis. (chromatographyonline.com)
Kinase6
- Protein kinase C phosphorylation promotes localization of PCTP to the mitochondrion where it may activate Them2. (wikipedia.org)
- Its lipid transfer activity is negatively regulated by casein kinase, a serine/threonine-protein kinase which phosphorylates STARD10 on a serine residue at position 284 (Olayioye et al. (reactome.org)
- Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. (lookformedical.com)
- for example, protein kinase activation is part of the platelet activation signal pathway. (lookformedical.com)
- Diacylglycerols are lipid second messengers that activate protein kinase C, promoting tumor growth. (chromatographyonline.com)
- The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)
Phosphatidylethanolamine2
- Spin-label ESR studies on avidin-biotinyl phosphatidylethanolamine in membrane recombinants with myelin proteolipid proteins. (mpg.de)
- These results show that membrane damage by NMDA is preceded by inhibition of phospholipid synthesis and not by phospholipid degradation in the early stages of the excitotoxic process, and that NMDA receptor overactivation decreases phosphatidylcholine and phosphatidylethanolamine synthesis by inhibiting choline-ethanolaminophosphotransferase activity. (jneurosci.org)
Vesicles4
- The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)
- The low reactivity of large vesicles is not due to the difference in vesicle number for large and small vesicles at constant total lipid, nor to occlusion of lipid surfaces in multilammelar structures, and can be reversed by addition of low ( (tamu.edu)
- According to the vesicle fusion model there are two distinct ER-derived vesicles in S. cerevisiae, one type of vesicle has the docking complex proteins (Pex13, Pex14, Pex17) and other vesicle contains the RING-finger complex proteins (Pex2, Pex10 and Pex12). (5dok.net)
- Growth and division model proposes that peroxisomes form by fission from pre-existing ones and peroxisomal membrane proteins (PMPs) containing vesicles from the ER fuse with them to form functional peroxisomes which can grow further and divide [12]. (5dok.net)
Synthesis5
- Current work centres around the synthesis and exploitation of labelled lipids and sterols. (dur.ac.uk)
- Is it exclusively for protein synthesis or does it do other processes more identified with the smooth endoplasmic reticulum? (biologyonline.com)
- How about lipid synthesis? (biologyonline.com)
- Peroxisomes are devoid of DNA and protein synthesis machinery. (5dok.net)
- The rate-limiting and regulated step in cholesterol synthesis is catalyzed by the smooth endoplasmic reticulum-membrane protein, hydroxymethylglutaryl coenzyme A (HMG CoA) reductase, which produces mevalonate from HMG CoA. (pharmacy180.com)
Inositol lipid1
- PITP plays an essential role in intracellular vesicle flow and inositol lipid signaling. (embl.de)
Phospholipid Transfer1
- Phospholipid transfer proteins in rat lung. (wikipedia.org)
Binds2
- An x-ray structure shows that the C-terminal domain of mitoguardin-2 has a hydrophobic cavity that binds lipids. (rupress.org)
- This bilirubin binds to albumin and is transported to the liver, where it is transferred into hepatocytes. (msdmanuals.com)
Endoplasmic reticulum4
- The N -terminal transmembrane domain of ACBP1 and ACBP2 targets each protein to the endoplasmic reticulum (ER) and the plasma membrane while ACBP3 is targeted extracellularly. (aocs.org)
- When ribosomes are seen attached to the endoplasmic reticulum, it means a protein molecule is being synthesized. (biologyonline.com)
- Without the ribosomes, the endoplasmic reticulum is most likely involved in other cellular processes, e.g., synthesizing lipids, detoxifying or metabolizing drugs and toxins, or regulating calcium ions. (biologyonline.com)
- How does the endoplasmic reticulum make proteins? (biologyonline.com)
Liver3
- Phosphatidylcholine transfer protein (PC-TP, synonym StARD2) is a highly specific intracellular lipid binding protein that is enriched in liver. (wustl.edu)
- Bile acid is transferred from fetus to mother through fetus circulation, and then mother exhausted the exceeding bile acid out of the body with normal liver and gall system. (hindawi.com)
- Galactosylated lipid substances allowed liver specific uptake of AZT at enhanced parenchymal: non-parenchymal selectivity ratios and at the same time could deliver MCZ for treatment of fungal infections. (ijpsr.com)
Apolipoproteins2
- Lipoproteins are composed of a neutral lipid (TAG, cholesteryl esters, or both) core surrounded by a shell of amphipathic apolipoproteins, phospholipid, and unesterified cholesterol. (pharmacy180.com)
- In contrast to plasma, CS-6253 had no effect on the assessed CSF apolipoproteins or lipids. (cdc.gov)
Cellular3
- The domain is named after cellular retinaldehyde-binding protein (CRALBP) and TRIO guanine exchange factor. (embl.de)
- The Rho family of GTP-binding proteins has been implicated in the regulation of various cellular functions including actin cytoskeleton-dependent morphological change. (embl.de)
- Despite the detailed in vitro characterization of the enzymatic properties of yeast Sac1p, the exact cellular function of this protein has remained obscure. (embl.de)
Cholesteryl esters1
- They function to keep lipids (primarily triacyl-glycerol [TAG] and cholesteryl esters) soluble as they transport them between tissues. (pharmacy180.com)
Hydrophobic3
- The CRAL-TRIO domain is found in GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs) and a family of hydrophobic ligand binding proteins, including the yeast SEC14 protein and mammalian retinaldehyde- and alpha-tocopherol-binding proteins. (embl.de)
- The structure also identified a hydrophobic binding pocket for lipid binding. (embl.de)
- The researchers also saw a third distinct OH vibrational signal: a water structure with H-up orientation in the hydrophobic lipid tail. (rdworldonline.com)
Bilayers1
- Distance measurements using paramagnetic ion-induced relaxation in the saturation transfer electron spin resonance of spin-labeled biomolecules: Application to phospholipid bilayers and interdigitated gel phases. (mpg.de)
STRUCTURES4
- Using this technique, known as heterodyne-detected vibrational sum frequency generation (HD-VSFG), the team has now discovered that water adopts three structures at a lipid interface containing atoms with both negative and positive charges, providing critical new evidence of "local" hydration arrangements around different membrane components. (rdworldonline.com)
- Tahara notes this is clear evidence of two different surface water structures: an 'H-down' orientation, with water's hydrogen atoms pointing away from the lipid interface, and the opposite 'H-up' arrangement. (rdworldonline.com)
- To pinpoint how these two structures arranged at zwitterionic interfaces, the researchers mixed together pure anionic and cationic lipid surfactants in varying amounts. (rdworldonline.com)
- Then using HD-VSFG, they revealed that H-up structures attached to negative phosphates through hydrogen bonding, while H-down structures bonded to positively charged amines-a new concept for phosphatidylcholine interfaces. (rdworldonline.com)
Triglycerides1
- Triglycerides and phosphatidylcholines were the major lipid components identified in both tissues and hemolymph, accounting for, respectively, 27% and 15% of all lipids in tissues and, respectively, 24% and 38% of all lipids in the hemolymph. (hindawi.com)
Cytosolic proteins1
- The three other ACBPs (ACBP4, ACBP5 and ACBP6) are cytosolic proteins. (aocs.org)
Soluble2
- This soluble protein is 214 amino acids long. (wikipedia.org)
- To that end, they conducted a large screen crossing lipid-sensing nuclear receptors and soluble lipid transporters. (anl.gov)
Peripheral3
- They consist of two types, peripheral and integral proteins. (lookformedical.com)
- Brewster angle microscopy has been used to characterise the association of peripheral membrane proteins with lipid monolayers (with Dr A Beeby, Durham University). (dur.ac.uk)
- Interaction of membrane-spanning proteins with peripheral and lipid-anchored membrane proteins: perspectives from protein- lipid interactions (Review). (mpg.de)
Cholesterol4
- Alteration of synaptic membrane cholesterol/phospholipid ratio using a lipid transfer protein. (mcw.edu)
- This protein therefore represents the first and rate-controlling step in the reverse cholesterol transport pathway. (vondellhenderson.com)
- For the metabolic syndrome in which multiple mild abnormalities in lipids, waist size (abdominal circumference), blood pressure, and blood sugar increase the risk of CHD, the designated HDL cholesterol levels that contribute to the syndrome are sex-specific. (medscape.com)
- If more cholesterol enters the bile than can be solubilized by the available bile salts and phosphatidylcholine, cholesterol gallstone disease (cholelithiasis) can occur. (pharmacy180.com)
Peptides2
- Recent work has begun to establish the factors that control the innate reactivity of peptides towards acyl transfer from membrane lipids (intrinsic lipidation). (dur.ac.uk)
- This technique offers label-free and rapid analysis of various molecular species, including lipids, metabolites, peptides, and drugs, making it a powerful tool in fields such as biomedical research, pharmaceutical analysis, and forensics. (chromatographyonline.com)
Biophysical1
- Using our methods, we can determine their precise biophysical properties to facilitate lipid binding and membrane remodeling. (jove.com)
Recombinant1
- Our methods can be used to understand the lipid specificities of all yeast SNX-BARs or to produce recombinant SNX-BAR proteins from any other organism. (jove.com)
Cytosol1
Anionic1
Pathways5
- Such protein-mediated transfer is especially important for maintaining organelles disconnected from secretory pathways, like mitochondria. (rupress.org)
- A transcriptomic study of sexual maturation in H. discus hannai has uncovered certain pathways related to maturation, with lipid pathways being crucial in this process [ 19 ]. (hindawi.com)
- That is accomplished by transferring factors that modulate different metabolic and signaling pathways. (frontiersin.org)
- Aberrations in lipid and lipoprotein metabolic pathways can lead to numerous diseases, including cardiovascular disease, diabetes, neurological disorders, and cancer. (cdc.gov)
- Then, we established protein-lipid topological networks for each metabolic category and linked dysregulated proteins and lipids with defined metabolic pathways. (cdc.gov)
Droplets1
- We identify mitoguardin-2, a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), as a lipid transporter. (rupress.org)
Neuronal2
- We showed previously that NMDA releases choline and inhibits incorporation of [ 3 H]choline into phosphatidylcholine before excitotoxic neuronal death. (jneurosci.org)
- We propose the hypothesis that small high-density lipoprotein (HDL) particles reduce the risk of Alzheimer's disease (AD) by virtue of their capacity to exchange lipids, affecting neuronal membrane composition and vascular and synaptic functions. (cdc.gov)
StarD21
- This protein also founds the StarD2 subfamily of proteins. (wikipedia.org)
Modulate2
- Both the decrease in the Ch/PL ratio and the increase in the lipid-to-protein ratio increase membrane "fluidity," lipid-to-protein ratio increase membrane "fluidity," which may modulate the vertical displacement and motional characteristics of the GABA transporter. (mcw.edu)
- The MARV matrix layer confers the virion filamentous shape and stability but how host lipids modulate mVP40 oligomerization is mostly unknown. (uci.edu)
Subfamily2
- This subfamily consists of PCTP, StarD7, StarD10 and collagen type IV alpha-3-binding protein or StarD11, all of which bind phosphatidylcholine except for StarD11 which prefers ceramide. (wikipedia.org)
- Members of a subfamily of these enzymes share a specific domain that was first identified in the yeast Sac1 protein [1]. (embl.de)
Spectroscopy2
- Lipid-protein interactions in ADP-ATP carrier/egg phosphatidylcholine recombinants studied by spin-label ESR spectroscopy. (mpg.de)
- Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. (ibecbarcelona.eu)
Transporter1
- One pair stood out: lipid transporter PCTP and nuclear receptor PPARdelta. (anl.gov)
Bile1
- Bile salts and phosphatidylcholine are quantitatively the most important organic components of bile. (pharmacy180.com)
Inhibition1
- The intracellular transfer of information (biological activation/inhibition) through a signal pathway. (lookformedical.com)
Transmembrane1
- Interactions between lipid-anchored and transmembrane proteins. (mpg.de)
Tissues3
- Therefore, in the present study, we performed untargeted lipid profiling of abalone tissues at different developmental stages as well as the hemolymph of mature female and male abalones using ultrahigh-performance liquid chromatography-tandem mass spectrometry. (hindawi.com)
- A total of 132 lipids belonging to 15 classes were identified from abalone tissues at different developmental stages. (hindawi.com)
- Of note, lysophosphatidylcholine was only detected in the tissues of mature abalones, paving the way for further analyses of abalone lipids based on developmental stages. (hindawi.com)
Abnormalities1
- The basic purpose of the management of HA and related lipid abnormalities is to reduce the risk of atherosclerosis, which is the main mechanism of increased morbidity and mortality. (medscape.com)
Family proteins2
- The guanine nucleotide exchange factor (GEF) Dbl targets Rho family proteins thereby stimulating their GDP/GTP exchange, and thus is believed to be involved in receptor-mediated regulation of the proteins. (embl.de)
- An amino-terminal portion conserved among a subset of Dbl family proteins is sufficient for the binding of Gbetagamma. (embl.de)
Chemical2
- Particular interests include the kinetics of peptide rearrangements following membrane binding 1 and the chemical stability of peptide-lipid systems. (dur.ac.uk)
- The membrane-binding properties of proteins from enveloped viruses are being characterised by a combination of microscopy, chemical biology and structral biology approaches (with Dr R. P. Yeo, Biological Sciences, Durham University). (dur.ac.uk)
Interactions5
- Finally, the researchers focused on the specific protein interactions driving the different phenotypes. (anl.gov)
- ACBP1 and ACBP2 each possess a C-terminal domain of ankyrin repeats, which facilitates protein-protein interactions. (aocs.org)
- We have used host-guest chemistry 5 to test the hypothesis that aromatic amino acids have thermodynamically significant interactions with the headgroups of neutral lipids such as phosphatidylcholines. (dur.ac.uk)
- Influence of polar residue deletions on lipid-protein interactions with the myelin proteolipid protein. (mpg.de)
- FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
Species2
- Excessive Ca 2 + entry through glutamate receptors activates a large array of potential neurotoxic mechanisms, which include activation of enzymes such as phospholipases, calpains and other proteases, protein kinases, nitric oxide synthetase, endonucleases, as well as reactive oxygen species (for review, see Sattler and Tymianski, 2000 ). (jneurosci.org)
- Based on the quantitative characterization of serum samples from 146 individuals, we have determined individual lipid species and proteins that were significantly up- or down-regulated relative to the normolipidemic group. (cdc.gov)
Choline1
- June 2, 2022 About half the dietary choline consumed in the United States is in the form of phosphatidylcholine [8,9]. (appyuntamiento.es)
Residue1
- A single-residue deletion alters the lipid selectivity of a K+ channel-associated peptide in the beta-conformation: Spin label electron spin resonance studies. (mpg.de)
Phosphorylation1
- It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation. (lookformedical.com)
Bind3
- X-ray crystallography shows that this domain forms a pocket that can bind a single molecule of phosphatidylcholine. (wikipedia.org)
- It's not a smoking gun-they would require very sophisticated mass spectrometry or kinetics to look at the transfer of phospholipid from one protein to another-but the researchers were able to support their hypothesis that the interaction between PCTP and PPARdelta is repressive, and when PCTP is unable to bind phosphatidylcholines, it is unable to interact with and repress PPARdelta. (anl.gov)
- Protein modules such as FVYE domains and PH domains that bind specifically to PtdIns 3-phosphate (PtdIns-3-P) and polyphosphoinositides, respectively, can direct such membrane targeting. (embl.de)
Risk of atherosclerosis1
- They are intended to reduce the risk of atherosclerosis (a cardiovascular disease) by improving blood lipid levels.At least three medications within this class have failed to result in benefits however. (vondellhenderson.com)
MRNA1
- Oval cells are induced between 1 and 3 days after PH inside the portal field and they express the typical onco-foetal marker alpha foeto-protein (AFP), at mRNA and protein levels. (uni-goettingen.de)
Bound2
- Small molecule inhibitors bound PC-TP, displaced phosphatidylcholines from the lipid binding site, and increased the thermal stability of the protein. (wustl.edu)
- The scientists solved the structure of PCTP bound to phosphatidylcholine, collecting crystallographic data at the Southeast Regional Collaborative Access Team (SER-CAT) beamline at 22-ID at the APS. (anl.gov)
Kinetics1
- Oppositely oriented Glt Tk proteins showed the same transport kinetics, consistent with the use of an identical gating element on both sides of the membrane. (nature.com)
Lipoprotein1
- The integration of quantitative lipid and lipoprotein profiling of human plasma may provide a powerful approach to inform early disease diagnosis and prevention. (cdc.gov)
Intestinal2
- This study aimed to investigate whether curcumin could inhibit intestinal and hepatic NPC1L1 expression through suppressing sterol regulatory element binding protein-2 (SREBP-2) / hepatocyte nuclear factor 1α (HNF1α) pathway, then exert anti-NASFL effects. (bvsalud.org)
- Chylomicrons are assembled in intestinal mucosal cells from dietary lipids (primarily TAG). (pharmacy180.com)
Disorders1
- Defects in the lysosomal breakdown of sphingolipids are the underlying cause of lipid storage disorders such as Niemann-Pick, Tay-Sachs, Krabbe and Gaucher diseases. (thermofisher.com)
Atherosclerosis1
- Mice lacking PCTP exhibit a resistance to atherosclerosis linked to changes in plasma lipid levels and changes in body weight linked to the level of brown fat use of fatty acids and Them2 activity. (wikipedia.org)
Cytoskeletal1
- Clathrin also interacts with cytoskeletal proteins. (lookformedical.com)
Transport3
- This is one of many tasks regulated by lipid transport proteins such as PCTP. (anl.gov)
- Their interaction was strong, selective, and new: PPARdelta was known to interact with lipid transport proteins but not phosphatidylcholine transport proteins. (anl.gov)
- transport proteins. (vondellhenderson.com)
Microscopy2
- The six Arabidopsis ACBPs are subcellularly located in various compartments based on experimental results using a variety of techniques including fluorescent protein tagging in confocal microscopy, immunoelectron microscopy and western blot analysis of subcellular fractions from wild-type plants as well as from transgenic plants expressing fluorescent-tagged ACBPs. (aocs.org)
- This has led to the structure determination for RSV M protein (in collaboration with Dr V. Money), 3 and studies of membrane bindign using tensiometry, electron microscopy, circular dichroism and Brewster angle microscopy. (dur.ac.uk)
Pctp3
- In humans, phosphatidylcholine transfer protein is encoded by the PCTP gene. (wikipedia.org)
- Further studies found that sensitivity to phosphatidylcholine levels causes PCTP to interact with select enzymes, promoting their activation. (wikipedia.org)
- An in-cell protein complementation screen uncovered a direct interaction between PCTP and PPARdelta that was not observed for other PPARs. (anl.gov)