• The reagents supplied in the Calcium Phosphate Transfection Kit (Product No. CAPHOS) are sterilized by 0.2 µM filter and aseptically filled. (sigmaaldrich.com)
  • We also offer DharmaFECT ® Duo Transfection Reagents, as well as RapidTrans™ chemically competent E. coli , which are supplied in a convenient 96-tube tray. (activemotif.com)
  • The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
  • Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
  • Improving on previous generations of FuGENE ® technology, FuGENE ® 4K provides efficient delivery of DNA into a wide range of cell lines while providing the gentle, low-toxicity transfection you've come to expect from FuGENE ® reagents. (selectscience.net)
  • Obtain higher transfection efficiencies than leading competitors' reagents while minimizing impacts on cell health to get the most out of your transfection-based experiments. (selectscience.net)
  • Invitrogen Corporation has introduced a key product into its line of transfection reagents. (technologynetworks.com)
  • A wide range of reagents for the biocompatible fusion-mediated transfection of various molecules into living cells. (ibidi.com)
  • TransIT®-293 Transfection Reagent - A High Efficiency, Low Toxicity, Plasmid DNA Transfection Reagent Optimized for 293 Cells ׀ Cell Line Specific DNA Transfection Reagents are formulated to maximize transfection efficiency while maintaining cellular health in many popular or hard-to-transfect cell types. (mobitec.com)
  • Broad range transfection reagents suitable for transfecting a broad range of different cell types, including mammalian and insect cells. (cambio.co.uk)
  • Avalanche® Transfection Reagent Series: Built on their expert knowledge & experience, powered by their vision & passion, EZ Biosystems™ has been dedicated to the research and development of next-generation transfection reagents by applying their expertise in combinatorial chemistry, molecular biology, and cell biology. (cambio.co.uk)
  • Check out the general purpose transfection reagents for plasmids, siRNAs (plasmid + siRNA co-transfections) and CRISPR/Cas9 as well as highly specific transfection reagents that have been carefully optimised for different primary cell types and different cell lines. (cambio.co.uk)
  • Each of the cell type/cell line-specific transfection reagents in our Avalanche ® series was individually formulated. (cambio.co.uk)
  • For 25 years we have led the way with our broad-spectrum DNA transfection reagents. (fugene.com)
  • Whether it's for life science research, the production of antibodies, proteins, viruses, or for groundbreaking therapeutic needs, FuGENE ® has you covered with our innovative transfection reagents. (fugene.com)
  • CLICK HERE to search our Reference Database which highlights 1000's of publications citing FuGENE ® DNA Transfection reagents. (fugene.com)
  • Getting DNA or RNA into cells can be a tricky business, and a variety of transfection reagents have been developed over the years to make the process easier. (promegaconnections.com)
  • Lipid-based reagents are especially popular because they combine efficient transfection with relatively low toxicity. (promegaconnections.com)
  • For several decades, transfection reagents have targeted this size range. (promegaconnections.com)
  • iMFectin Poly DNA Transfection Reagent is a powerful gene delivery compound, which enables the achievement of higher efficiencies in the delivery of DNA into eukaryotic cell lines and primary cells compared to other DNA transfection reagents. (gendepot.com)
  • iMFectin Poly DNA Transfection Reagent is very gentle on cells since it requires low amounts of reagents during transfection. (gendepot.com)
  • The Effectene Transfection Reagent is intended for molecular biology applications. (qiagen.com)
  • Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. (qiagen.com)
  • Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. (qiagen.com)
  • Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. (qiagen.com)
  • In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. (qiagen.com)
  • Here we describe transient protein production using QMCF Technology (Icosagen, Estonia) that uses specific suspension-adapted mammalian cell lines (QMCF cells), appropriate QMCF episomal expression vector, a chemically defined animal origin-free, serum-free medium, and Reagent 007™ (Icosagen, Estonia) for efficient delivery of nucleic acids for transfection of mammalian cells in comparison with PEI MAX™ (Transfection Grade Linear Polyethylenimine Hydrochloride, Polysciences). (springer.com)
  • Karro K, Männik T, Männik A, Ustav M (2015) DNA transfer into animal cells using stearylated CPP based transfection reagent. (springer.com)
  • Trans IT™-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types and increase recombinant lentivirus production. (fishersci.com)
  • Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
  • When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
  • Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. (thermofisher.com)
  • Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
  • This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
  • Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
  • The reagent allows for transfection to be performed both in presence or absence of serum. (thermofisher.com)
  • The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
  • We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
  • FuGENE® 4K Transfection Reagent from Promega Corp. (selectscience.net)
  • A best-in-class DNA transfection reagent for your research. (selectscience.net)
  • FuGENE ® 4K is the newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into challenging and routine mammalian cell lines. (selectscience.net)
  • LipoD293™ In Vitro DNA Transfection Reagent-1.0 mL - 1 kit is backordered and will ship as soon as it is back in stock. (dnamethsoc.com)
  • Lipofectamine™ LTX Reagent delivers DNA into the cell and demonstrates high levels of transfection efficiency and protein expression with 90 percent viability for a wide range of cells, including primary neuronal cells and other disease-relevant cell types. (technologynetworks.com)
  • Lipofectamine™ RNAi MAX is a RNAi-specific transfection reagent that is designed to offer high transfection efficiency and low toxicity profiles on a wide variety of cell types for siRNA gene knockdown experiments. (technologynetworks.com)
  • TransIT®-293 Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. (mobitec.com)
  • Transfections with TransIT-293 Reagent do not require medium changes. (mobitec.com)
  • With the launch of Avalanche® Transfection Reagent series, EZ Biosystems™ has been at the leading forefront of next-generation transfection reagent development and production. (cambio.co.uk)
  • Avalanche ® - primary cell type-specific Transfection Reagent series? (cambio.co.uk)
  • GeneMedi provide LipoGene TM transfection reagent which is highly potent cationic lipofection reagent that has been shown to effectively transfect plasmids or siRNA, as well as nucleic acid-protein complexes, into cultured adherent and suspension cell lines. (genemedi.com)
  • 3. Mix the LipoGene liposome transfection reagent gently. (genemedi.com)
  • Poznání, že prakticky všechny transfekční metody jsou ovlivněny obranyschopností vrozeného imunitního systému, vedlo k vývoji K4® Transfection System sestávajícího z K4® Transfection Reagent a K4® Multiplier. (biogen.cz)
  • K4® Transfection Reagent je založeno na silných kationtových lipidech, zatímco K4® Multiplikátor snižuje schopnost buněk detekovat cizí nukleové kyseliny. (biogen.cz)
  • FuGENE ® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines. (fugene.com)
  • What transfection reagent/method will work best for your cell type and experiment? (promegaconnections.com)
  • Transfection can sometimes seem more like an art than a science-the perfect transfection experiment being dependent on optimization of conditions, including cell density, transfection reagent and DNA:reagent ratio. (promegaconnections.com)
  • EZFect™ Transfection Reagent is a unique polymer blend that can provide cells with more DNA and siRNA at a lower cost than leading lipid-based transfection kits. (creative-biolabs.com)
  • EZFect™ is a powerful and widely applicable transfection reagent that enables efficient and repeatable transfection. (creative-biolabs.com)
  • Each batch of EZFect™ transfection reagent was functionally tested in the transfection test of human embryonic kidney 293 cells. (creative-biolabs.com)
  • Thank you for your interest in EZFect™ Transfection Reagent . (creative-biolabs.com)
  • This innovative transfection reagent contains a blend of biodegradable polymer based delivery compounds, which facilitate transfection of nucleic acid without any toxic effect. (gendepot.com)
  • iMFectin Poly DNA Transfection Reagent is powerful tool in delivering DNA to various established cell lines as well as primary cells. (gendepot.com)
  • The procedure stated below is designed for the transfection of CHO cells with 1 µg/µL pSV40-CAT plasmid (diluted in sterile molecular biology grade water). (sigmaaldrich.com)
  • Typically, most of the cells that have not integrated the transfected plasmid will die while the cells that have undergone plasmid integration will survive by 9 days post-transfection. (mirusbio.com)
  • Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. (oregonstate.edu)
  • However, this technology relies heavily on the insertion of foreign plasmid DNA into the nuclei of cells (i.e., transfection), which can be very challenging and highly variable among cell types. (oregonstate.edu)
  • Maximal transfection efficiency was obtained with 0.3μL/well of LipoLTX plus 25ng/well of plasmid DNA (ca. 29.5±1.9%) and 0.15μL/well of LipoLTX plus 25ng/well of plasmid DNA (ca. 4.0±0.4%) for MACT and MDBK cells, respectively. (oregonstate.edu)
  • Since the cell type, culture conditions, transfection parameters, etc. will greatly af ect the transfection ef iciency, it is necessary to optimize the plasmid volume/LipoGene volume within the recommended range. (genemedi.com)
  • Analysis was performed 48 hours post transfection utilizing flow cytometry to determine percent transfection efficiency and the intensity of GFP expression. (thermofisher.com)
  • This method can be employed if a detectable marker is expressed on the cells post-transfection. (mirusbio.com)
  • Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)
  • If LipoGene displayed toxicity to the targetcells, it is recommended to remove the antibiotics from the transfection system. (genemedi.com)
  • Designed for delivery of DNA of all sizes, it offers high-efficiency transfection with extremely low toxicity in over 800 cell lines. (fugene.com)
  • Find mRNA transfection Kits with low toxicity at BOC Sciences! (bocsci.com)
  • Polyplus-transfection, a leading biotechnology company that supports the gene and cell therapy market by supplying innovative transfection solutions , named Mario Philips as Chief Executive Officer. (biopharmanalyses.fr)
  • Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. (wikipedia.org)
  • In animal cells, transfection is the preferred term as transformation is also used to refer to progression to a cancerous state (carcinogenesis) in these cells. (wikipedia.org)
  • Transfection of animal cells typically involves opening transient pores or "holes" in the cell membrane to allow the uptake of material. (wikipedia.org)
  • Transfection can result in unexpected morphologies and abnormalities in target cells. (wikipedia.org)
  • The original meaning of transfection was "infection by transformation", i.e., introduction of genetic material, DNA or RNA, from a prokaryote-infecting virus or bacteriophage into cells, resulting in an infection. (wikipedia.org)
  • For work with bacterial and archaeal cells transfection retains its original meaning as a special case of transformation. (wikipedia.org)
  • Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term propagation in culture, or acquisition of properties typical of cancer cells), the term transfection acquired, for animal cells, its present meaning of a change in cell properties caused by introduction of DNA. (wikipedia.org)
  • Broadly defined, transfection is the process of artificially introducing nucleic acids ( DNA or RNA ) into cells , utilizing means other than viral infection. (newworldencyclopedia.org)
  • Commonly, the term transfection is used in the sense of non-viral introductions of nucleic acid specifically into eukaryotic cells, or even more specifically into animal cells. (newworldencyclopedia.org)
  • Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term propagation in culture, or acquisition of properties typical of cancer cells), the term transfection acquired, for animal cells, its present meaning of a change in cell properties caused by introduction of nucleic acids by non-viral methods. (newworldencyclopedia.org)
  • 1990) state that the meaning of transfection is "clear and unambiguous" and refers to the "process by which nucleic acid (DNA or RNA) is introduced to cells with the intent to be expressed in the recipient. (newworldencyclopedia.org)
  • The transfection process is different from "infection," which is the term used for a viral method of introducing nucleic acids into cells (Promega). (newworldencyclopedia.org)
  • Transformation of eukaryotic cells in tissue culture is usually called transfection. (newworldencyclopedia.org)
  • Since the DNA introduced in the transfection process is usually not inserted into the nuclear genome, the foreign DNA is lost at the later stage when the cells undergo mitosis . (newworldencyclopedia.org)
  • If it is desired that the transfected gene actually remains in the genome of the cell and its daughter cells, a stable transfection must occur. (newworldencyclopedia.org)
  • The expression of the target gene is regulated only in the cells with double transfection by the two vectors. (news-medical.net)
  • Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. (sigmaaldrich.com)
  • Here, we prepared a novel nanoparticle made by polymers which may solve the problem of targeting transfection into glioblastoma cells. (cns.org)
  • All the pre- and post-modified PEI nanoparticles augmented the transfection efficiency of U87 cells, but the latter was better. (cns.org)
  • Vectors based on VTW peptide and PEI can improve gene transfection efficiency of U87 glioblastoma cells notably and specially. (cns.org)
  • It is unclear the reason for the low transfection in MDBK cells. (oregonstate.edu)
  • Using high, medium, and low transfection efficiency conditions determined in Experiment 1, we performed a GRT assay for peroxisome proliferator-activated response element (PPRE) luciferase in MACT and MDBK cells treated with 10nM or 100nM of synthetic Peroxisome Proliferator-activated Receptor β/σ (PPARβ/σ) agonist. (oregonstate.edu)
  • The GRT assay was unaffected by poor transfection in MACT cells although the high transfection hampered the possibility of detecting differences between 10 and 100nM of the PPARβ/δ agonist. (oregonstate.edu)
  • Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. (cambio.co.uk)
  • The day before transfection (20-24 hours) ~0.4×10 6 cells (the specific cell number depends on the cell size and cell growth rate), were inoculated into a six-well plate, so that they would be 70-90% confluent the next day at the time of transfection. (genemedi.com)
  • LipoGene-DNA mixtures are usually suf icient to produce high transfection ef iciency when incubated with cells for six hours. (genemedi.com)
  • However, the replacement of fresh medium 6 hours after transfection can improve the transfection ef iciency of some cells which grow very fast. (genemedi.com)
  • For some easily transfected cells such as HEK-293T cells, whether change of transfection solution or not depends on cell growth status, and it is not necessary to change the transfection solution to improve the transfection ef iciency. (genemedi.com)
  • FuGENE ® knows how important the high efficiency and gentle DNA transfection of your cells is to your research & production. (fugene.com)
  • Leveraging our gentle and highly efficient proprietary transfection chemistry, FuGENE ® 4K provides efficient and safe entry of DNA into a wide range of cells. (fugene.com)
  • mRNA transfection is a novel solution for difficult-to-transfect cells such as neurons, primary cells and suspension cells. (bocsci.com)
  • New reverse genetics and transfection methods to rescue arboviruses in mosquito cells. (cdc.gov)
  • It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
  • Transient transfection of intracellular Notch1 (Notch1-IC) into a pre-B cell line resulted in the down-regulation of EBF-regulated promoters and diminished the capacity of EBF to activate these promoters in an epithelial cell line. (lu.se)
  • The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
  • Far better transfection efficiency than Lipofectamine® 2000 when we compared them head-to-head. (thermofisher.com)
  • Transfection also has been used to refer to infecting a cell specifically with viral nucleic acid that is either isolated from a eukaryote virus or from a bacteriophage . (newworldencyclopedia.org)
  • In transfection, the introduced nucleic acid may be transient, such that it does not replicate, or it may be stable and integrate into the genome of the recipient and replicate when the host genome replicates. (newworldencyclopedia.org)
  • Her research involves methods as tissue microarray (TMA) of colon cancer patients for immunohistochemistry (IHC) and Western blot, qPCR, transfection, luciferase assay, immunofluorescens on colon cancer cell lines. (lu.se)
  • Question: For this particular experiment (mRNA transfection), are there any specific biochemical modifications that the mRNA should have? (biosyn.com)
  • K4® Transfection System je proto vhodný pro přechodnou a stabilní transfekci savčích buněčných linií a primárních buněk DNA (plazmidy, bacmid), RNA (mRNA, miRNA a siRNA) a modifikovanými nukleovými kyselinami (např. (biogen.cz)
  • Adding serum into transfection medium produced fewer influence to modified PEI nanoparticles compared with lipofectamine2000. (cns.org)
  • High serum level with antibiotics usually do not have inhibitory effect on transfection efficiency. (gendepot.com)
  • This technique has been used to obtain both transient 1 and stable 2 transfections in a wide variety of cell types. (sigmaaldrich.com)
  • It is suitable for both transient and stable transfection and can be used for many applications such as gene expression, viral production, shRNA expression, and promoter analysis. (mobitec.com)
  • Do you want to do transient (short-term) transfections, or are you going to establish a stable cell line? (promegaconnections.com)
  • Investigating the epi-miRNome: identification of epi-miRNAs using transfection experiments. (bvsalud.org)
  • Chemicals include methods such as lipofection, which is a lipid-mediated DNA-transfection process utilizing liposome vectors. (wikipedia.org)
  • We propose a pipeline to prioritize candidate epi-miRs and to identify potential epigenetic interactors of any given miR starting from miR transfection experiment datasets. (bvsalud.org)
  • Chemical-based transfection can be divided into several kinds: cyclodextrin, polymers, liposomes, or nanoparticles (with or without chemical or viral functionalization. (wikipedia.org)
  • The collaborative research team led by Professor Tadashi ISA, Project Assistant Professor Masaharu KINOSHITA from The National Institute for Physiological Sciences, The National Institutes of Natural Sciences and Fukushima Medical University and Kyoto University, developed 'the double viral vector transfection technique' which can deliver genes to a specific neural circuit by combining two new kinds of gene transfer vectors. (news-medical.net)
  • The key component of this discovery was 'the double viral vector transfection technique'in which one vector is retrogradely transported from the terminal zone back to the neuronal cell bodies and the other is transfected at the location of their cell bodies. (news-medical.net)
  • Professor Tadashi ISA says 'this newly developed double viral vector transfection technique can be applied to the gene therapy of the human central nervous system, as we are the same higher primates. (news-medical.net)
  • Transfection can be carried out using calcium phosphate (i.e. tricalcium phosphate), by electroporation, by cell squeezing, or by mixing a cationic lipid with the material to produce liposomes that fuse with the cell membrane and deposit their cargo inside. (wikipedia.org)
  • The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. (qiagen.com)
  • Transfection & expression of recombinant DNA are frequently used to study the effects and regulation of genes and their encoded proteins. (activemotif.com)
  • Longo PA, Kavran JM, Kim M-S, Leahy DJ (2013) Transient mammalian cell transfection with polyethylenimine (PEI). (springer.com)
  • Other physical methods use different means to poke holes in the cell membrane: Sonoporation uses high-intensity ultrasound (attributed mainly to the cavitation of gas bubbles interacting with nearby cell membranes), optical transfection uses a highly focused laser to form a ~1 µm diameter hole. (wikipedia.org)
  • There are many methods employed in performing transfection. (newworldencyclopedia.org)
  • C-terminal polypeptide produced by transfection studies inhibits myogenesis. (medscape.com)
  • They are easy to culture and are commonly used in transfection and large-scale production of therapeutic proteins. (thermofisher.com)
  • Surprisingly, PITX2A activation of the ANF and PLOD1 promoters is repressed by co- transfection of Nkx2.5 in the C3H10T1/ 2 embryonic fibroblast cell line. (lu.se)
  • Transfection Culture Medium is a complete medium specially designed for cell transfection. (ubigene.us)
  • All four steps in the transfection process were optimized and our most advanced lipid nanoparticle technologies were combined. (thermofisher.com)
  • Antibiotics, Glutamine, etc. have no ef ect on LipoGene transfection. (genemedi.com)