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  • gene
  • Importantly, the gene transfection activity and cellular uptake of SP-DNA complexes were all markedly higher than that of complexes of polyethyleneimine 25,000 and DNA in MCF-7 and MCF-7/ADR cell lines. (dovepress.com)
  • Different applications of gene electrotransfer require high viability of cells and high transfection efficiency of gene electrotransfer. (springer.com)
  • It was already demonstrated that the addition of fetal bovine serum (FBS) immediately after gene electrotransfer leads to improved cell survival and transfection efficiency. (springer.com)
  • On the other hand, transfection efficiency of gene electrotransfer was not affected by long incubation of cell in FBS, regardless of the cell line used. (springer.com)
  • The results of our study emphasize the importance of optimization of gene electrotransfer protocol for particular cells and specific purposes of gene electrotransfer, taking into account the importance of transfection efficiency and cell survival. (springer.com)
  • cell
  • Therefore, the aim of the study was to determine whether prolonged incubation of cells in FBS, for more than standard 5 min, can lead to increased transfection efficiency and improved cell survival. (springer.com)
  • Transfection efficiency was determined by flow cytometry and fluorescence microscopy and cell survival by cell viability assay. (springer.com)