• Neuronal transfection in brain slices using particle-mediated gene transfer. (nih.gov)
  • Difficulties in neuronal transfection continue to restrict the applicability of molecular approaches to neurobiology. (nih.gov)
  • We describe here an alternative strategy using particle-mediated gene transfer for the transfection of neurons and glia in intact brain slices. (nih.gov)
  • Conventional transfection techniques have been of limited effectiveness, particularly in intact neural tissues. (nih.gov)
  • Browse our X-tremeGENE™ Transfection Database to easily find the best reagent for your specific applications, cell types, references and published protocols. (sigmaaldrich.com)
  • Transfection protocols can be established in smaller scale using the 4D-Nucleofector™ X Unit and smoothly transferred to larger scale without the need for re-optimization. (lonza.com)
  • By combining a Nucleofector™ Device with Lonza's easy-to-use Nucleofector™ Kits and the company's range of cell-type specific protocols, high transfection efficiencies can be reproducibly achieved with excellent cell viability. (lonza.com)
  • Successful transfection of THP-1 monocytes with subsequent phorbol 12-myristate 13-acetate (PMA)-induced differentiation into macrophages is not a trivial matter, because according to previous transfection protocols, cell viability is lost almost completely within 24 h of PMA treatment following transfection. (biomedsearch.com)
  • MATra can also be adapted to high-throughput transfection assays using robotic stations and adapted protocols. (promocell.com)
  • We have tried a number of transfection protocols. (ncifcrf.gov)
  • As long as the individual transfection protocols are compatible, this method ensures uptake of all nucleic acids by the cell simultaneously. (mirusbio.com)
  • 80% cell viability) at the optimal transfection concentration by MTT assay. (rsc.org)
  • Here we demonstrate how a Design of Experiment (DOE) approach can be quickly automated on the Biomek i7 Automated Workstation (Figure 1) to identify optimal transfection conditions for a variety of cell lines. (beckman.com)
  • Several cell densities and lipid volumes were investigated to determine optimal transfection conditions, shown by the shaded boxes. (horizondiscovery.com)
  • NeuroPORTER provides a fast, simple, and reliable system for DNA transfections and assures high efficiency, high viability results every time! (sigmaaldrich.com)
  • Low transfection efficiency and low cell viability are the most frequent causes of unsuccessful gene silencing experiments. (bio-medicine.org)
  • The goal of transfection optimization is to determine the conditions that will provide maximum gene knockdown while maintaining an acceptable level of viability for the particular cell type (see sidebar, Two-Step Optimization Protocol ). (bio-medicine.org)
  • It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types with improved cell viability. (thermofisher.com)
  • Parameters that were optimized included the DNA amount, the DNA-to-PEI ratio, the timing and solution conditions for complex formation, the transfection medium, and the cell density at the time of transfection. (epfl.ch)
  • Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection. (atcc.org)
  • Furthermore, the F-CDs shows excellent efficient transfection even at low pDNA dose, low F-CDs transfection reagent dose and high serum concentration, indicating potential clinical applications. (rsc.org)
  • For ease of use, plasmid transfections can be carried out entirely in the presence of serum . (clontech.com)
  • Promote transfection using lipofection and support growth of HEK 293 cultures with GE Healthcare HyClone SFM4Transfx-293 media, a serum-free, animal-derived component free (ADCF) media. (thomassci.com)
  • MATra transfections can be performed in the presence or absence of serum and/or antibiotics. (promocell.com)
  • Transfection of RNA can be used either to induce protein expression, or to repress it using antisense or RNA interference (RNAi) procedures. (qiagen.com)
  • The first set of slides demonstrates the efficient transfection of a plasmid expressing GFP (green fluorescent protein) into a primary culture of rat cortical neurons. (sigmaaldrich.com)
  • We are truly delighted to sign this license agreement with Kempbio, a company offering quality cell culture and protein expression services," said Mark Bloomfield, CEO of Polyplus-transfection. (biospace.com)
  • Protein/antibody delivery using PULSin may be superior to transfection in some applications. (bio-medicine.org)
  • Virus-mediated transfection, also known as transduction, offers a means to reach hard-to-transfect cell types for protein overexpression or knockdown, and it is the most commonly used method in clinical research (Glover et al. (thermofisher.com)
  • What is the Xfect Protein Transfection Reagent and how does it work? (clontech.com)
  • Xfect Protein Transfection Reagent is a modified peptide with cell-penetrating activity whose amino acid composition enables it to interact with a protein cargo and transport this protein across a cell membrane barrier. (clontech.com)
  • Protein transfection is extremely rapid compared to traditional gene expression studies using transfected DNA (1-2 hours compared to 18-48 hours), because it bypasses cellular processes such as transcription and translation. (clontech.com)
  • What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies? (clontech.com)
  • With recessive dystrophic epidermolysis bullosa keratinocytes (RDEBK-TA4), the DSP exhibited high transfection efficacy with both Gaussia luciferase marker DNA and the full length COL7A1 transcript encoding the therapeutic type VII collagen protein (C7). (rsc.org)
  • Cell transfection promotes the rapid advancement of our knowledge of genetic regulation and protein function. (promocell.com)
  • This is not (usually) a problem if the vector carries a gene for a nontoxic protein and the vector stock has been freshly prepared by transfection. (ncifcrf.gov)
  • Transfection technology application market is further segmented as biomedical research, therapeutic delivery and protein production. (sbwire.com)
  • Rising prevalence of various cancers (prostate, breast and lung), cardiovascular diseases (arrhythmias, ischemic heart disease) and growing mass protein production further accentuates the global transfection technology market. (sbwire.com)
  • Transfection technologies are widely used in cell research, recombinant protein production, drug discovery, gene therapy and therapeutics. (sbwire.com)
  • The data from the four transfection experiments were combined for gene expression profiling data analysis, as previously described . (bionity.com)
  • Polyplus-transfection SA is a biotechnology company researching, developing, manufacturing and marketing innovative solutions for scientists working in molecular and cell biology. (biospace.com)
  • Polyplus-transfection recently extended its product offering to molecular biologists with the launch and commercialization of ZNA (TM) modified oligonucleotides. (biospace.com)
  • Successful transfection is influenced by many factors. (qiagen.com)
  • Apart from the cell type, successful transfection also depends on the culture's age and density at transfection, the vector used, the purity of the nucleic acids, and the experimental conditions. (promocell.com)
  • Step by step, using this technology, researchers have been able to bring more functionality to cell types that were considered to be refractory to transfection, such as primary neurons," states Dr. Berger. (genengnews.com)
  • We describe here an alternative strategy using particle-mediated gene transfer for the transfection of neurons and glia in intact brain slices. (nih.gov)
  • transfection of nucleic acids into primary neurons and cultured neuronal cell lines. (thomassci.com)
  • The findings have been published in ( ACS Applied Materials & Interfaces , 'Effect of PEGylated Magnetic PLGA-PEI Nanoparticles on Primary Hippocampal Neurons: Reduced Nanoneurotoxicity and Enhanced Transfection Efficiency with Magnetofection' ). (nanowerk.com)
  • Furthermore, they found increased gene transfection efficiency in primary hippocampal neurons using PEGylated MNP-PLGA-PEI nanoparticles under an external magnetic field. (nanowerk.com)
  • there is a growing demand for new transfection technologies to address unmet needs for therapeutic delivery which is driving the transfection market. (marketsandmarkets.com)
  • Growth in gene therapy is also driving the transfection market. (sbwire.com)
  • Transfection signals from native CAT-encoding DNA compared well with those from a synthetic DNA sequence adapted to the P. falciparum major codon bias, demonstrating effective expression of the bacterial sequence despite its use of rare P. falciparum codons. (pnas.org)
  • High levels of dystrophin cDNA expression, and an efficient distant transfection effect with preferential intranuclei inclusion of MF-2 vehicle, are very encouraging for the development of a new constructive strategy in gene therapy trials of DMD. (nature.com)
  • Stable expression can be influenced by two factors: The transfection method used and the vector containing the shRNA of interest. (openwetware.org)
  • The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. (pnas.org)
  • Effectene Reagent is used in conjunction with the enhancer and the DNA condensation buffer (Buffer EC) to achieve high transfection efficiency. (wikipedia.org)
  • In this webinar, we discuss the key steps and protocol considerations for performing high-quality transfections on a consistent basis, taking into consideration variables such as cell type, culture conditions, reagent concentrations, and other critical parameters. (sciencemag.org)
  • Rentia I have used Promega's Wizard minipreps and maxipreps to prepare plasmid DNA that was suitable for transfection. (bio.net)
  • Explore the resources provided below to easily identify which X-tremeGENE™ transfection reagent is suitable for your specific cell line and application needs. (sigmaaldrich.com)
  • It is also suitable for assaying tissue sections after in vivo transfection. (activemotif.com)
  • This is true for most of the reagent based transfection reactions. (marketsandmarkets.com)
  • Reagent based transfection technology market is experiencing significant growth owing to cost effectiveness and less complicated methods involved during transfection. (yahoo.com)
  • Experiments involving large constructs have typically been carried out using electroporation, as reagent-based transfection yielded poor results. (thermofisher.com)
  • Market Research Report Search Engine Added 'Transfection Technology Market (Reagent-based method, Instrument-based method and Virus-based method) - Global Industry Analysis, Size, Share, Growth, Trends and Forecast, 2013 - 2019' to its database. (sbwire.com)
  • There are few upcoming transfection technologies like nucleofection and magnetofection which are an off-shoot of electroporation, which have the potential to address these challenges but are currently cost prohibitive due to which the technologies may have a much slower uptake in the developing world. (marketsandmarkets.com)