• Prostaglandin E2 as transduction enhancer affects competitive engraftment of human hematopoietic stem and progenitor cells. (bvsalud.org)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). (stemcell.com)
  • The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. (stemcell.com)
  • This single-chain, 105-120 kDa, heavily O-glycosylated protein is expressed on hematopoietic progenitor cells, vascular endothelium, bone marrow stromal cells and embryonic fibroblasts. (bdbiosciences.com)
  • MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
  • LVs efficiently transduce slowly dividing cells, including hematopoietic stem-progenitor cells (HSCs), resulting in stable gene transfer and expression. (johnshopkins.edu)
  • I am interested in how the process of haematopoietic development in stem and progenitor populations is dysregulated by these genes in AML. (cardiff.ac.uk)
  • Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
  • She interconnects Cell, Progenitor cell, Protein domain, Enhancer and Hematopoietic stem cell in the investigation of issues within Transduction. (research.com)
  • The study incorporates disciplines such as Cell culture, Progenitor cell, CD34, Molecular biology and Transduction in addition to Haematopoiesis. (research.com)
  • Here, we performed global microarray-based gene expression measurements on human CD34 + hematopoietic stem and progenitor cells that underwent editing. (biu.ac.il)
  • 2018) describe the global transcriptional response to individual components of Cas9/AAV6-mediated genome editing in hematopoietic stem and progenitor cells. (biu.ac.il)
  • Here, we performed global microarray-based gene expression measurements on human CD34+ hematopoietic stem and progenitor cells that underwent editing. (biu.ac.il)
  • Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. (stemcell.com)
  • Soen Y, Mori A, Palmer TD, Brown PO "Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. (openwetware.org)
  • Several HOX transcription factors, including HOXB4, are highly expressed in hematopoietic stem cells (HSCs) but are downregulated upon differentiation towards more mature progeny cells. (dissertations.se)
  • Adenoviral mediated overexpression of HOXB4 in human primitive hematopoietic cells in vitro generates very high levels of HOXB4 within 24 hours of transduction, leading to an increased differentiation towards myeloid lineages, and to a reduction of primitive hematopoietic progenitors, suggesting that the level of HOXB4 expression may determine the fate options following gene transfer. (dissertations.se)
  • Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
  • NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. (novusbio.com)
  • PURPOSE OF REVIEW: We provide a summary of the temporal cascade of transcriptional networks giving rise to the hematopoietic stem cell (HSC) and controlling differentiation of the erythroid lineage from it. (ox.ac.uk)
  • Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
  • In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
  • We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
  • Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
  • moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
  • These results suggest that Syp may be an important signaling component downstream of the EpoR and may regulate the proliferation and differentiation of hematopoietic cells. (drugbank.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Moreover, studies with nonmyeloablated murine recipients show that transplantation of vector-exposed, washed hematopoietic cells results in systemic dissemination of functional VSV-G/lentivector particles. (elsevierpure.com)
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
  • An ideal transduction protocol should determine high gene marking in long-term self -renewing hematopoietic stem cells (HSCs), preserving their repopulation potential during in vitro manipulation. (bvsalud.org)
  • The addition of PGE2 shortly before transduction of human CD34+ cells determined a significant transduction increase in the in vitro cell progeny paralleled by a significant reduction of their clonogenic potential. (bvsalud.org)
  • Blockage of CXCR4 with AMD3100 (plerixafor, Mozobil) did not affect transduction efficiency but partially rescued CD34+ clonogenic impairment in vitro. (bvsalud.org)
  • Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
  • FUS/TLS has also been proposed to bind human telomeric RNA (UUAGGG)4 and single-stranded human telomeric DNA in vitro. (wikipedia.org)
  • We obtained bone marrow, isolated CD34(+) cells, performed in vitro transduction with a retroviral vector carrying a rev-responsive element (RRE) decoy gene, and reinfused the cells into these subjects with no evidence of adverse effects. (nih.gov)
  • Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
  • Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
  • Using a single LV containing two constitutive promoters, we achieved strong and sustained expression of both transgenes in transduced engrafting CD34 + HSCs and their progeny, as well as in other human cell types. (johnshopkins.edu)
  • Hematopoietic stem cells (HSCs) are functionally defined as cells that upon transplantation into irradiated or otherwise immunocompromised adult organisms provide long-term reconstitution of the entire hematopoietic system. (ox.ac.uk)
  • This research work was carried out to determine the effect of prolonged fasting on age-induced and chemotherapy-induced damage to hematopoietic stem cells (HSCs). (drcarney.com)
  • Researchers found out that multiple cycles of prolonged fasting decreased the concentrations of insulin-like growth factor (IGF-1) and the activity of protein kinase A (PKA) .Diminished IGLF levels and PKA activity were observed to activate signal transduction in long-term hematopoietic stem cells (LT-HSCs) that promoted the rejuvenation of old and damaged stem cells. (drcarney.com)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
  • The cytoplasmic region of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting CD34 may play a role in signal transduction. (bdbiosciences.com)
  • We conducted a gene therapy trial in 10 patients with adenosine deaminase (ADA)-deficient severe combined immunodeficiency using 2 slightly different retroviral vectors for the transduction of patients' bone marrow CD34(+) cells. (duke.edu)
  • Once transplanted in vivo in a competitive repopulation assay, human CD34+ cells transduced with PGE2 contributed significantly less than cells transduced with a standard protocol to the repopulation of recipient mice , indicating a relative repopulation disadvantage of the PGE2 -treated CD34+ cells and a counter-selection for the PGE2 -treated cell progeny in vivo. (bvsalud.org)
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
  • Human immunodeficiency virus type 1-derived lentivirus vectors bearing the vesicular stomatitis virus G (VSV-G) envelope glycoprotein demonstrate a wide host range and can stably transduce quiescent hematopoietic stem cells. (elsevierpure.com)
  • Studies presented here instead reveal prolonged particle adherence after ex vivo exposure, despite serial wash procedures, with subsequent transduction of secondary target cells in direct and transwell cocultures. (elsevierpure.com)
  • Genetically engineered hematopoietic stem cells are a promising therapeutic strategy for treating a variety of conditions including HIV infection, cancer, and inherited disorders such as hemophilia and thalassemia's. (seattlechildrens.org)
  • A common method of transferring new genes or gene-editing components into cells, to provide novel functions, is by transduction of engineered lentiviral or other vectors. (seattlechildrens.org)
  • Dr. Torbett and collaborators are improving transduction of hematopoietic stem cells to increase delivery efficiency. (seattlechildrens.org)
  • They have identified multiple small molecules that facilitate the entry of viral vectors into mouse and human cells. (seattlechildrens.org)
  • For example, Dr. Torbett's group is studying polyphenols as transduction enhancers based on their results showing that the resveratrol-like compound caraphenol A dose-dependently increases gene delivery and genomic integration into human cells. (seattlechildrens.org)
  • The small compounds boost transduction into a variety of cells including other types of stem cells and T cells for CAR T therapy. (seattlechildrens.org)
  • In addition to improving gene therapy and delivery of gene-editing tools into cells, increasing transduction efficiency using small molecule agents could facilitate using of hemopoietic stem cells for protective functions. (seattlechildrens.org)
  • Research Objective A Treatment for Artemis-deficient Severe Combined Immunodeficiency using Non-Viral CRISPR-driven Safe Harbor Transgenesis in Hematopoietic Stem Cells Impact We aim to develop a novel genome editing based therapy for Artemis-deficient severe combined immunodeficiency that would improve upon prior gene therapies in efficacy, safety, and scalability. (ca.gov)
  • Participates in signal transduction in hematopoietic cells. (abcam.com)
  • CD81 has a very broad cellular distribution, being expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin. (bdbiosciences.com)
  • However, the relative low transduction efficiency in human long-term hematopoietic stem cells limits the utility of these vectors in this cell type. (stjude.org)
  • Researchers at St. Jude have discovered that overexpression of a truncated form of the human HMGA2 gene in a lentiviral vector can cause transduced hematopoietic stem cells to proliferate in transplanted animal models. (stjude.org)
  • By incorporating the HMGA2 gene, the small number of hematopoietic stem cells that were initially transduced (about 1%) expanded over a period of two years to become 40-70% of the stem cell population in transplant recipients. (stjude.org)
  • This approach can be used to achieve in vivo expansion of hematopoietic stem cells transduced with lentiviral vectors that carry any desired therapeutic gene, such as gamma-globin, or to enhance genome editing efficiency. (stjude.org)
  • leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. (stemcell.com)
  • In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. (stemcell.com)
  • A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. (stemcell.com)
  • Enforced expression of HOXB4 dramatically increases the regeneration of murine HSC following transplantation, and enhances the repopulating ability of human SCID repopulating cells. (dissertations.se)
  • Developmental potential of human haematopoietic stem cells in the NOD/Scid xenotransplantation model. (ucl.ac.uk)
  • To investigate the developmental potential of human stem cells I used xenotransplantation of stem cells purified from cord blood into a mouse model. (ucl.ac.uk)
  • Human stem cells introduced into the NOD/Scid mouse after partial myeloablation repopulate the bone marrow and contribute to the hematopoietic system of the mouse. (ucl.ac.uk)
  • To allow better identification of human cells transduction with a lentivirus carrying the GFP gene was used. (ucl.ac.uk)
  • I conclude that transdifferentiation of human stem cells to a mature hepatocyte phenotype does not occur in the NOD/Scid model of bone marrow transplantation even after severe CCU induced liver damage. (ucl.ac.uk)
  • Instead human cells most probably of haematopoietic origin fuse with resident hepatocytes to give rise to mixed heterokaryons expressing some, but not all markers of human hepatocytes. (ucl.ac.uk)
  • Genetic modification of hematopoietic stem cells with genes that inhibit replication of human immunodeficiency virus-1 (HIV-1) could lead to development of T lymphocytes and monocytic cells resistant to HIV-1 infection after transplantation. (nih.gov)
  • These observations support the potential of performing gene therapy for HIV-1 using hematopoietic cells, but emphasize the need for improved gene transfer techniques. (nih.gov)
  • SV40-based vectors can transduce human hepatic and hematopoietic cells. (huji.ac.il)
  • We studied the effect of HBV on the transduction - efficiency of human hepatic cells by SV40 - based vectors. (huji.ac.il)
  • Methods: A SV40-vector carrying the luciferase gene, and wild-type SV40, were used to assess transduction efficiency of human HBV-positive and HBV-negative hepatic cells. (huji.ac.il)
  • To evaluate whether differences in transduction efficiency are due to cell recognition and/or nuclear transport, MHC-I receptors were measured by FACS analysis and SV40-DNA was extracted from the nuclei of transduced cells and quantified. (huji.ac.il)
  • Transient transfection of HuH-7 cells with the HBV genome also increased transduction efficiency. (huji.ac.il)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • Moreover, CD201 + hematopoietic cells remained in a relatively slow cycling state, consistent with high expression levels of P57 and Ccng2. (biomedcentral.com)
  • In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
  • Successful engraftment of gene-corrected hematopoietic stem cells in non-conditioned patients with Fanconi anemia. (research.com)
  • We found that Syp, a phosphotyrosine phosphatase, widely expressed in all tissues in mammals became phosphorylated on tyrosine after stimulation with Epo in M07ER cells engineered to express high levels of human EpoR. (drugbank.com)
  • Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Prolonged fasting can protect hematopoietic stem cells from aging and cancer chemotherapy. (drcarney.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)
  • Early reports indicated cell engraftment from bone marrow transplants into non- hematopoietic tissues in mouse to mouse and in human allogeneic bone marrow transplants. (ucl.ac.uk)
  • Engraftment into the bone marrow was measured by FACS and human genetic material was found in the spleen, skin, lung and liver. (ucl.ac.uk)
  • However, transdifferentiation defined as the emergence of mature human hepatocytes after bone marrow transplantation could not be found. (ucl.ac.uk)
  • The haematopoietic system provides an exemplar whereby characterisation of the blood lineages during development and the bone marrow niche has resulted in therapeutics now routinely used in the clinic. (ox.ac.uk)
  • Diehn M, Bhattacharya R, Botstein D, Brown PO "Genome-Scale Identification of Membrane-Associated Human mRNAs. (openwetware.org)
  • A variety of methods used to determine the genetic identity in GFP positive hepatocytes only identified remnants of the human genome. (ucl.ac.uk)
  • In recent years, the availability of the human genome sequence disclosed novel opportunities to study biological processes from a higher level perspective. (biomedcentral.com)
  • Conclusions: HBV increases transduction by SV40-vectors. (huji.ac.il)
  • The study incorporates disciplines such as Cancer research, Virology, Wiskott-Aldrich syndrome, Viral vector and Transduction in addition to Genetic enhancement. (research.com)
  • Genetic enhancement, Cell biology, Haematopoiesis, Viral vector and Transduction are her primary areas of study. (research.com)
  • Her research in Genetic enhancement intersects with topics in Hematopoietic stem cell transplantation, Cancer research, Wiskott-Aldrich syndrome and Prospective cohort study. (research.com)
  • Anne Galy mainly focuses on Genetic enhancement, Haematopoiesis, Viral vector, Cancer research and Hematopoietic stem cell transplantation. (research.com)
  • Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
  • We develop a computational model for the hematopoietic erythroid-myeloid lineage decision, which is determined by a genetic switch involving the genes PU.1 and GATA-1. (lu.se)
  • Two subjects were restarted on ERT because of poor gene marking and immune recovery, and one had a subsequent allogeneic hematopoietic stem cell transplantation. (duke.edu)
  • Allogeneic hematopoietic stem cell transplantation in the COVID-19 era. (cdc.gov)
  • 2022. Increased expression of RUNX3 inhibits normal human myeloid development . (cardiff.ac.uk)
  • Citation: Chickarmane V, Enver T, Peterson C (2009) Computational Modeling of the Hematopoietic Erythroid-Myeloid Switch Reveals Insights into Cooperativity, Priming, and Irreversibility. (lu.se)
  • These multiple possibilities support future use of combinations of compounds to synergistically increase transduction efficiency. (seattlechildrens.org)
  • Transduction efficiency was measured as luciferase activity or by T-antigen staining. (huji.ac.il)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • The analysis of genomic expression during human myelopoiesis using an integrative computational approach allowed discovering important relationships between genomic position, biological function and expression patterns and highlighting chromatin domains, including genes with coordinated expression and lineage-specific functions. (biomedcentral.com)
  • Human peripheral blood lymphocytes were stained with CD230 (clone 3F4) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram). (biolegend.com)
  • While the JAK-STAT pathway is indispensable for normal cellular functions, it was also discovered that abnormal activation of the JAK-STAT signaling pathway significantly contributes to the formation and progression of many human carcinomas. (innspub.net)
  • Well-illustrated and clinically focused, it details the basic science and clinical practice of hematology and hematopoietic cellular therapy-covering virtually all aspects of hematology in one definitive resource. (elsevierhealth.com)
  • Studies in our knockout mice reveal that deficiency of HOXB4 leads to a hypocellularity in hematopoietic organs and impaired proliferative capacity. (dissertations.se)
  • Mice and humans were studied for this research work. (drcarney.com)
  • RNA-binding protein FUS/TLS (FUsed in Sarcoma/Translocated in LipoSarcoma), also known as heterogeneous nuclear ribonucleoprotein P2 is a protein that in humans is encoded by the FUS gene. (wikipedia.org)
  • FUS/TLS was initially identified as a fusion protein (FUS-CHOP) produced as a result of chromosomal translocations in human cancers, especially liposarcomas. (wikipedia.org)
  • Klapholz-Brown Z, Walmsley GG, Nusse YM, Nusse R, Brown PO "Transcriptional program induced by wnt protein in human fibroblasts suggests mechanisms for cell cooperativity in defining tissue microenvironments. (openwetware.org)
  • The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. (biolegend.com)
  • We probed effects of the entire editing process as well as each component individually, including electroporation, Cas9 (mRNA or protein) with chemically modified sgRNA, and AAV6 transduction. (biu.ac.il)
  • The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). (rndsystems.com)
  • In the context of the improvement of a clinically applicable transduction protocol, we tested prostaglandin E2 ( PGE2 ) as a transduction enhancer (TE). (bvsalud.org)
  • I lead a multi-disciplinary research group focused on abnormalities affecting haematopoietic (blood cell) development which leads to haematological malignancies (blood cancer). (cardiff.ac.uk)
  • Cancer is the second most common cause of death in the United States and the leading cause of deaths worldwide and thus remains a global problem to the human population. (innspub.net)
  • Molecular subtypes of small cell lung cancer: a synthesis of human and mouse model data. (cancerindex.org)
  • pendent of those associated with cretory ducts, columnar epithelia the KRAS gene is more frequent- other key mechanistic characteris- lining the gastrointestinal tract and ly mutated in human cancer, which tics of IARC Group 1 carcinogens. (who.int)
  • Notch signaling is essential for hematopoietic stem cell (HSC) formation during embryogenesis, and hitherto it was also thought to be required for HSC maintenance. (ox.ac.uk)
  • Prolonged fasting reduces IGF-1/PKA to promote hematopoietic stem cell based regeneration and reverse immunosuppresion. (drcarney.com)
  • Ischaemic heart disease is a major cause of morbidity and mortality in humans and the question remains as to whether these principles can be applied to the heart, in order to exploit the potential of adult SCs for use in cardiovascular repair and regeneration. (ox.ac.uk)
  • For many gene transfer applications, transduction of more than one gene is needed. (johnshopkins.edu)
  • 2022. RUNX3 overexpression inhibits normal human erythroid development . (cardiff.ac.uk)