Transcriptional targets. Medical search. Frequent questions

After being produced, the stability and distribution of the different transcripts is regulated (post-transcriptional regulation) by means of RNA binding protein (RBP) that control the various steps and rates controlling events such as alternative splicing, nuclear degradation (exosome), processing, nuclear export (three alternative pathways), sequestration in P-bodies for storage or degradation and ultimately translation. (wikipedia.org)
Composed of heterodimers, they share a common HIF-1β/ARNT subunit and similar DNA-binding motifs, raising the possibility of crosstalk between the two transcriptional pathways. (ox.ac.uk)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Target genes, variants, tissues and transcriptional pathways influencing human serum urate levels. (nih.gov)
Her research studies the signaling pathways and transcriptional events that mediate GBM SCs growth. (hopkinsmedicine.org)
Ongoing work is focused on defining molecular pathways underlying inflammation-mediated transcriptional and epigenetic regulation of HSCs. (bcm.edu)
Targeted transcriptional profiling showed no effects on pathways involved inmitochondrial biogenesis, function, or oxidative stress. (medscape.com)
In summary, we have identified, and at the molecular level characterized, signaling pathways and specific factors through which oncogenic HIF2A activity is regulated and may be targeted. (lu.se)
The goal of this initiative is to support research aimed at (1) identification of targets and pathways by which transcriptional activity of HIV can be suppressed in HIV reservoirs including the CNS in people with SUD, and (2) application of emerging small molecule drug discovery approaches to identify novel compounds that can be utilized as pharmacological probes and as drugs to suppress HIV transcription in people with HIV-SUD comorbidity. (cdc.gov)

This leads to Phase-III of this research programme in which the most powerful targeted vectors will be chosen to be developed further, by inserting novel powerful therapeutic genes into them. (europa.eu)
The purpose of our study was to use an unbiased approach to determine whether hypertensive individuals on conventional therapy exhibit an altered monocyte gene expression profile and to perform validation studies of selected genes to identify novel therapeutic targets for hypertension. (nih.gov)
Furthermore, we describe stimulus-specific regulation of a subset of these target genes by p53. (biomedcentral.com)
The most salient differentiation-relevant target genes included wnt7b involved in dendritic extension and the tfcp2l4/grhl3 grainyhead homolog implicated in ectodermal development. (biomedcentral.com)
As a transcription factor, many of the functional cellular roles of the p53 protein are elicited through direct DNA binding to sequence-specific cis-regulatory elements [ 3 ] leading to the transactivation or transrepression of target genes. (biomedcentral.com)
In contrast, the Fushi Tarazu homeodomain protein is an activator, both in cultured cells and in Drosophila embryos, where it activates several known target genes, including its own gene. (biologists.com)
Normal Fushi Tarazu target genes are affected as they are in fushi tarazu mutants. (biologists.com)
SMAD4 target genes are part of a transcriptional network that integrates the response to BMP and SHH signaling during early limb bud patterning. (bvsalud.org)
SMAD4 regulates gene expression in response to BMP and TGFß signal transduction , and is required for diverse morphogenetic processes, but its target genes have remained largely elusive. (bvsalud.org)
Here, we identify the SMAD4 target genes in mouse limb buds using an epitope -tagged Smad4 allele for ChIP-seq analysis in combination with transcription profiling. (bvsalud.org)
Most importantly, our analysis reveals a predominant function of SMAD4 in upregulating target genes in the anterior limb bud mesenchyme . (bvsalud.org)
We hypothesized that MDM2 in these cells inhibited transcription initiation at the p53 target genes p21 and puma . (oncotarget.com)
Surprisingly, following etoposide treatment transcription initiation occurred at the compromised target genes in MANCA and A875 cells similar to the T/T ML-1 cell line. (oncotarget.com)
We found that knockdown of MDM2 in G/G cells moderately increased expression of subsets of p53 target genes without increasing p53 stability. (oncotarget.com)
We show that the TC-AR translocates to the nucleus, activates transcription of AR target genes in the absence of DHT and is sufficient to confer ADI growth to the normally androgen dependent LNCaP line. (tmu.edu.tw)
We also show that while there is significant overlap in the genes regulated by FL- and TC-AR there are also differences in the respective suites of target genes with each AR form regulating genes that the other does not. (tmu.edu.tw)
A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals. (babraham.ac.uk)
Although AmlR apparently is a local transcriptional regulator of the aml operon, the Δ amlR strain shows severe growth inhibitions on glucose and - concomitantly - differential transcription of several genes of various functional classes. (frontiersin.org)
It promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. It also acts as a transcription repressor of E2F1 target genes. (nih.gov)
ARNT-dependent HIF-2 transcriptional activity is not sufficient to regulate downstream target genes in neuroblastoma. (lu.se)

This systematic approach revealed post-transcriptional regulation of metabolism following BRAF inhibition, involving selective mRNA transport and translation. (biorxiv.org)
Thus, these studies identify the HRC enhancer as the first MEF2-dependent, CArG-independent transcriptional target in smooth muscle and represent the first analysis of the transcriptional regulation of an SR gene in vivo. (ox.ac.uk)
We hypothesized that p53 contributed to PC12 differentiation through the regulation of gene targets distinct from its known transcriptional targets for apoptosis or DNA repair. (biomedcentral.com)
We present evidence that SYD is recruited to the WUS promoter and that it is involved in regulation of the stem cell pool maintenance via direct transcriptional control of this master regulator. (upenn.edu)
Post-transcriptional regulation is the control of gene expression at the RNA level. (wikipedia.org)
Essentiality of the Maltase AmlE in Maltose Utilization and Its Transcriptional Regulation by the Repressor AmlR in the Acarbose-Producing Bacterium Actinoplanes sp. (frontiersin.org)
We provide a new mechanism for the regulation of heart development by PITX2 isoforms through the regulation of ANF and PLOD1 gene expression and Nkx2.5 transcriptional activity. (lu.se)
As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
We found that the regulation of TE transcription during brain development is essential for the establishment of long-term transcriptional repression carried to adulthood (Paper I and IV). (lu.se)

in an operon with an upstream PurR/LacI-type transcriptional regulator gene, named amlR ( ACSP50_2475 ), and a gene downstream ( ACSP50_2473 ) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. (frontiersin.org)

One such target gene is repressed by EFE even where Fushi Tarazu is not expressed, suggesting that the repression is active. (biologists.com)
It recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. (nih.gov)

Despite data supporting the transcriptional activity of p53 protein in differentiation, few genetic targets have been described and fewer still within a neuronal context. (biomedcentral.com)
Discovery of non-genotoxic mechanisms through which p53 protein may be activated within tumor cells harboring wild-type p53 remains an important therapeutic objective, both in effort to alleviate traditional off-target chemotherapeutic side-effects and to prevent the unpredictable mutagenicity characteristic of these therapies. (biomedcentral.com)
The Engrailed homeodomain protein is an 'active' or dominant transcriptional repressor in cultured cells. (biologists.com)
The tiny ubiqwitin-like modifier protein (SUMO) regulates transcriptional activity and the translocation of proteins across the nuclear membrane1. (cell-signaling-pathways.com)
Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
Key proteins involved in lipoprotein metabolism, such as PCSK9, angiopoietin-related protein 3, cholesteryl ester transfer protein and apolipoprotein(a), have been identified as viable targets for therapeutic intervention through observational and genetic studies. (cdc.gov)
These proteins can be targeted using a variety of approaches, such as protein inhibition or interference, inhibition of translation at the mRNA level. (cdc.gov)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
In line with our previous results, we found that there are marked differences between HD patients and controls at the protein level but not at the transcriptional level. (lu.se)
It regulates the activities of PROTEIN PHOSPHATASE-1 and PROTEIN KINASE A, and it is a key mediator of the biochemical, electrophysiological, transcriptional, and behavioral effects of DOPAMINE. (bvsalud.org)
By combinatorial screening using H4 and molecules with defined targets, we demonstrated that H4 induces differentiation by the activation of protein kinase C (PKC) signaling pathway, and in line with this, activates PKC phosphorylation and translocation of PKC to the cell membrane. (lu.se)

Here, we examined how targeted therapy reprograms metabolism in BRAF-mutant melanoma cells using a genome-wide RNAi screen and global gene expression profiling. (biorxiv.org)
Upon depletion of RBPJ using CRISPR/Cas9, we observed specific upregulation of Notch target gene expression. (mdpi.com)

The maintenance of transcriptional states regulated by histone modifications and controlled switching between these states are fundamental concepts in our understanding of nucleosome-mediated epigenetic memory. (babraham.ac.uk)
We have also discovered that infection can be a driver of Dnmt3a-loss of function clonal hematopoiesis, and defined the transcriptional and epigenetic mechanisms underlying the Dnmt3a-loss of function clonal selection advantage in the setting of chronic inflammation. (bcm.edu)

The gene encodes a transcriptional activator and is found mutated in ～11% of diffuse large B cell lymphomas (DLBCLs) and ～12% of follicular lymphomas. (researchassistantresume.com)

and 3) to define pathogenetic mechanisms, possibly unveiling novel therapeutic targets. (unibo.it)
Identification of novel therapeutic targets, stratified by histological as well as mutational and transcriptional phenotypes, should transform survival prospects. (lu.se)

The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing 1 - 10 . (cdc.gov)
Following crRNA and tracrRNA hybridization, SpCas9 is targeted to genomic loci matching a 20-nt guide sequence within the crRNA, immediately upstream of a required 5′-NGG protospacer adjacent motif (PAM) 11 . (cdc.gov)

HRC is a direct transcriptional target of MEF2 during cardiac, skeletal, and arterial smooth muscle development in vivo. (ox.ac.uk)
Together these two conserved domains in the N-terminal half of MEF2B direct DNA binding homodimerization of MEF2 polypeptides and interaction with specific transcriptional co-factors. (researchassistantresume.com)
The highly divergent C-terminal half of MEF2 proteins has been suggested to modulate their transcriptional activity17 18 The spectrum of targets activated by MEF2 transcription factors in different cell types is dependent on association with specific co-repressors and co-activators in response to multiple signaling pathways17. (researchassistantresume.com)

2012). Nevertheless, understand- ing H2A.Z recruitment may shed light on how PRCs are targeted to specific loci. (researchgate.net)
100 predicted genomic off-target loci in 293T and 293FT cells. (cdc.gov)

Furthermore, PI3K/Akt/mTOR pathway emerged as novel potential therapeutic target. (unibo.it)
Hence, IL-17F may play an orchestrating role in the pathogenesis of asthma and may provide a valuable therapeutic target for development of novel strategies. (hindawi.com)
Conclusion: VGLL3 is overexpressed in GC tissues and associated with a poor prognosis, indicating its potential as a novel prognosis biomarker and therapeutic target for GC. (researchgate.net)

The persistence and transcriptional reactivation of HIV lead to the development of neuropathological complications. (cdc.gov)

Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. (mdpi.com)
Design We performed whole-genome transcriptional analysis of colonic biopsies from patients with histologically active and inactive UC, and non-inflammatory bowel disease (non-IBD) controls. (bmj.com)
To facilitate mammalian genome engineering applications, we provide a web-based software tool to guide the selection and validation of target sequences as well as off-target analyses. (cdc.gov)
Both crRNA-tracrRNA duplexes and sgRNAs can be used to target SpCas9 for multiplexed genome editing in eukaryotic cells 1 , 3 . (cdc.gov)

Previously, we have demonstrated that NPAS3 regulates VGF at the transcriptional level. (frontiersin.org)

XPNPEP3 is a novel transcriptional target of canonical Wnt/β-catenin signaling. (nih.gov)

We show that Sirt1 deacetylase activity directly targets neurons to mediate neuroprotection from mutant huntingtin. (harvard.edu)
We identified BDNF as an important target of Sirt1 and TORC1 transcriptional activity in normal and HD neurons. (harvard.edu)

Using a specific example-transcriptional silencing by Polycomb-mediated H3K27 methylation-we demonstrate how to construct and simulate a stochastic histone modification model. (babraham.ac.uk)

These models, in conjunction with quantitative experimental data-ChIP data, quantification of mRNA levels, and single-cell fluorescence tracking in clonal lineages-can generate predictions that drive more targeted experiments, allowing us to understand mechanisms that would be challenging to unravel by a purely experimental approach. (babraham.ac.uk)
Taken together, our results suggest that post-transcriptional mechanisms play an important role in the brain both during development and in the adult brain. (lu.se)

This rare disorder results from mutation of the transcriptional. (msdmanuals.com)

Using genetic and molecular analyses we have identified WUSCHEL (WUS) as a biologically important target of the SNF2-class ATPase SPLAYED (SYD) in the shoot apical meristem of Arabidopsis. (upenn.edu)
This will allow for defining the transcriptional programs that govern HSC fate and explain the molecular basis for leukemogenesis. (lu.se)
By characterization of the genomic, transcriptional, and DNA methylation landscape in lung cancer subgroups defined by histology and other clinicopathological factors in both own and public cohorts we search for new molecular subgroups of potential clinical relevance, additional targets for synergistic treatment, and a deepened understanding of the molecular pathogenesis. (lu.se)

Cyclin K as a direct transcriptional target of the p53 tumor suppressor. (uchicago.edu)

For mechanistic insight, we investigated global transcriptional alterations in the target organ (lung) as well as several extrapulmonary tissues (heart, aorta, whole blood cells) following inhalation (40 mg/m3 for 3 h/d for 5 d a week for 10 d) to stainless steel welding fume. (cdc.gov)

The characteristics, efficiency, and toxicity of targeted vectors will first be assessed in vitro on relevant cell lines and primary cell cultures, then in vivo using animal models of brain and liver cancer. (europa.eu)
Post-transcriptional silencing of HMGA1 suppressed metastasis in vivo . (bl.uk)
The responding samples to H4, displayed increased expression of myeloid markers, a clear decrease in the nuclear-cytoplasmic ratio and the potential of re-activation of the monocytic transcriptional program reducing leukemia propagation in vivo. (lu.se)

Transcriptional targeting of transgenes expression to tumour cells will be obtained using tumour cell type specific promoter. (europa.eu)
Our general objective is the development of new generations of targeted viral vectors for the treatment of liver and brain cancer by directing viral entry and by restricting transgene expression. (europa.eu)
The expression of HRC in cardiac, skeletal, and smooth muscle raises the possibility of a common transcriptional mechanism governing its expression in all three muscle cell types. (ox.ac.uk)
In this study, we identified a transcriptional enhancer from the HRC gene that is sufficient to direct the expression of lacZ in the expression pattern of endogenous HRC in transgenic mice. (ox.ac.uk)
Transcriptional analyses of adult and pediatric adamantinomatous craniopharyngioma reveals similar expression signatures regarding potential therapeutic targets. (cornell.edu)
We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

SNF2 chromatin-remodeling ATPases play an important role in ensuring proper development in higher eukaryotes by controlling accessibility of cis-regulatory DNA regions to transcription factors and to the transcriptional machinery. (upenn.edu)
Transcriptional corepressor that binds to a number of transcription factors. (abcam.com)

As such, potential therapeutic targets identified in pediatric ACP are also likely to have relvance for adult patients. (cornell.edu)
Moreover, we highlight the potential therapeutic targets of cellular senescence in diabetic nephropathy and provide important clues for clinical strategies. (hindawi.com)
Furthermore, we will explore the potential therapeutic targets of cellular senescence and provide important clues for clinical strategies in the management of DN. (hindawi.com)

Using our compiled transcriptome dataset of 27 pediatric and 9 adult ACPs, obtained through the Advancing Treatment for Pediatric Craniopharyngioma Consortium, we interrogated potential age-related transcriptional differences using several rigorous mathematical analyses. (cornell.edu)

We conclude that receptor-mediated p53 transcriptional activity is involved in PC12 differentiation and may suggest a contributory role for p53 in neuronal development. (biomedcentral.com)
p53 transcriptional activity may be further involved in the differentiation of other non-neuronal cell types. (biomedcentral.com)

Individual partners will either provide transgenes, or regulatory transcriptional cassettes, which will be assembled into individual novel targeted recombinants. (europa.eu)
Their mutagenic potential and gene regulatory effect have shaped the evolution of transcriptional networks involved in development, pluripotency, and inflammation. (lu.se)

Consequently, in TNBC there is a clear need of identifying new targets for selective inhibition to improve patient outcome. (lu.se)

DKC1 has been found to be a direct target of the c- myc oncogene, strengthening the connection between DKC and malignancy. (medscape.com)
DKC1 is a direct and conserved transcriptional target of c-MYC. (medscape.com)

However, little is known about the downstream targets of PITX2 in cardiogenesis. (lu.se)

As proof of concept we demonstrate the RNA binding kinase UHMK1 interacts with mRNAs that encode metabolic proteins and selectively controls their transport and translation during adaptation to BRAF targeted therapy. (biorxiv.org)

We studied the transcriptional role of p53 during nerve growth factor-induced differentiation of the PC12 line into neuron-like cells. (biomedcentral.com)

Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. (abcam.com)

Ultimately we will use this information to therapeutically target LSCs and thereby improve the treatment of leukemia. (lu.se)

Although a subset of DLBCL patients can be cured a substantial fraction of them (～40%) die of the disease3 indicating the need to develop more specific targeted therapies. (researchassistantresume.com)
In a subset of individuals, a muscle biopsy was performed for transcriptional profiling via quantitative reverse transcription polymerase chain reaction and microarrays. (medscape.com)

Once new generation targeted viral vectors are generated, they will be sent to the appropriate partners for the in vitro testing of the efficiency of the novel vectors in various cell lines. (europa.eu)

To date, this patient subgroup has often had a poorer clinical outcome, early relapses, and without obvious available targeted therapies compared to other breast subgroups. (lu.se)

Targeted gene deletion mutants of amlE and amlR were constructed by use of the CRISPR/Cas9 technology. (frontiersin.org)

Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects. (cdc.gov)

Results Microarray analysis (n=43) demonstrates that UC patients in remission present an intestinal transcriptional signature that significantly differs from that of non-IBD controls and active patients. (bmj.com)

We identify a new transcriptional signature associated with the involved mucosa of UC patients in remission that is significantly different from that of uninvolved or non-inflammatory bowel disease mucosa. (bmj.com)

However, the biological targets controlled by these ATPases are largely unknown. (upenn.edu)

Thus, we will develop novel targeted viral vectors for applications to cancer gene therapy. (europa.eu)

The workprogram has been divided in 6 workpackages each corresponding to a targeted vector (retro-, lenti- or adeno-viral) tested in a specific tumour model (liver or brain). (europa.eu)

In this review, we discuss the finding that IL-17F has a key role in asthma pathology and is a novel drug target for asthma. (hindawi.com)
These studies suggest a key role of Sirt1 in transcriptional networks in normal and HD brain and offer an opportunity for therapeutic development. (harvard.edu)
Investigate the role for super-enhancers in defining LSC heterogeneity and their potential as therapeutic targets for LSCs. (lu.se)

We evaluated muscle mitochondrial function and associated transcriptional profiles in nonobese humans after 12 months of CR. (medscape.com)

Anatomy26

Organisms12

Diseases10

Chemicals and Drugs86

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes64

Disciplines and Occupations1

Information Science4

Health Care1

Pathways9

Genes20

Regulation9

Regulator1

Repression2

Protein11

Gene expression2

Epigenetic2

Activator1

Novel therapeutic targets2

Genomic loci2

MEF23

Loci2

Therapeutic Target3

Reactivation1

Genome4

Regulates1

Canonical1

Neurons2

Histone1

Mechanisms2

Mutation1

Molecular3

Tumor1

Tissues1

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Expression6

Transcription factors2

Potential therapeutic targets3

Differences1

Activity2

Regulatory2

Inhibition1

Direct2

Downstream1

Proteins1

Differentiation1

Activation1

Leukemia1

Subset2

Cell1

Therapies1

Mutants1

Cells1

Analysis1

Identify1

Largely1

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Specific1

Role3

Function1